In industrial and logistics settings, the use of soft and rigid spinal exoskeletons has been increasing. However, under a unified assistance level and comparable work scenarios, systematic comparisons of their effects on users’ thermophysiological responses and subjective thermal perceptions remain limited. Twenty male participants performed manual handling tasks under three load conditions (5, 10, and 15 kg) in three experimental conditions: without the exoskeleton (WEXO), a rigid exoskeleton (REXO), and a soft exoskeleton (SEXO). Metabolic rate, mean skin temperature (MST), thermal comfort vote (TCV), and thermal sensation vote (TSV) were measured. The key findings are as follows: Compared with WEXO, both exoskeletons significantly reduced metabolic rate. Across all loads, SEXO yielded a lower metabolic rate than REXO and showed a more gradual linear increase as the load increased, whereas REXO exhibited a larger rise at 15 kg. Overall, MST was higher in REXO than in SEXO. Wearing an exoskeleton was often associated with increased skin temperature at 5–10 kg, yet MST decreased for both exoskeletons at 15 kg. Subjective ratings further indicated better TCV and TSV with SEXO than with REXO, with the difference more pronounced under higher loads. Taken together, under the conditions of this study, the soft exoskeleton appears to better balance assistive benefits and thermal comfort. Nevertheless, its heat transfer and heat dissipation performance should be further optimized in future designs.