Black crust and spalling are common deterioration phenomena affecting marble relics, yet their correlation remains inadequately understood. Hyperspectral imaging, reflectance spectroscopy, portable X-ray Fluorescence (p-XRF), infrared thermography, Scanning Electron Microscopy coupled with Energy-Dispersive Spectroscopy (SEM-EDS), and microbiological analysis was employed to connect these two types of deterioration on the Danbi stone carving of the Confucian Temple in Beijing. Spectral and thermal analyses reveal that black crust significantly reduces reflectance and increase solar absorption by 27%, resulting in thermal stress. p-XRF and SEM-EDS analyses indicated that black crust is enriched in Fe, Ti, Zn, Pb, As and clay minerals, while spalling areas display increase Ca, reflecting substrate exposure. Microscopy reveals microcracks at the layer–substrate interface. Microbiological analyses identify Cladosporium anthropophilum and Alternaria alternata as contributors to surface-darkening. These multi-scale datasets collectively demonstrate that alterations in surface chemistry and bio-mediated darkening promoting the formation of black crusts, which subsequently induce marble spalling due to solar absorption and thermal stress. These findings clarify the coupled physical–chemical–biological pathways through which black crust accelerates stone spalling.

The Qianlong Stone Classics are the largest and best-preserved ensemble of officially commissioned stone inscriptions of Confucian classics extant, yet their stele bases are currently threatened by salt efflorescence. Fluctuations in ambient temperature and humidity contribute significantly to this deterioration. Taking Stele 17 as a representative case, this study assesses the risks of surface condensation and moisture-induced salt phase transitions through integrated temperature–humidity monitoring, infrared thermography, and soluble salt analysis. The risk of condensation remains low under typical conditions, as the stele base surface temperature exceeds the dew point by at least 0.5 °C. However, risks of salt deliquescence and hydration are substantial. The stone surface contains elevated levels of soluble salts, including four highly soluble species (sodium sulfate, calcium nitrate, sodium nitrate, and sodium chloride) and one moderately soluble species (calcium sulfate). Deliquescence phase transition humidities are approximately 50.5% for calcium nitrate, 74.3% for sodium nitrate, and 75.4% for sodium chloride, while sodium sulfate exhibits a hydration phase transition near 81%. Exhibition Hall humidity fluctuates around these critical thresholds, driving repeated dissolution–crystallization and hydration–dehydration cycles that progressively erode the stone microstructure. These hygrothermal cycles exhibit pronounced seasonal patterns, with frequent air-conditioning operation in summer amplifying thermal and humidity impacts. This study elucidates an air-moisture-driven salt deterioration mechanism distinct from classical capillary rise, clarifies the persistent progression of efflorescence in transitional seasons, and provides a scientific basis for optimizing environmental control strategies.

Stone relics exposed to outdoor environments frequently experience surface deterioration, with red stains being a common and persistent issue. The stains often observed on marble and limestone surfaces arise from complex interactions involving chemical reaction, pollutant deposition, and microbiological process. Although microbial colonization has been associated with biodeterioration, the specific mechanisms remain poorly understood. This study focuses on the red stains found on the Danbi marble carvings at Beijing Confucian Temple and the Imperial College. Combining microbial cultivation, molecular identification (ITS sequencing), SEM-EDS (Scanning Electron Microscopy), Raman spectroscopy, and HPLC-MS (high-performance liquid chromatography with mass spectrometry), we identified the pigment-producing fungus Lizonia empirigonia as the dominant agent, with no evidence of inorganic contributors such as iron/lead oxides. Metabolite profiling revealed flavonoids and polyketides as key coloring material, while controlled infection experiments demonstrated the fungus’s reliance on exogenous organic matter rather than direct stone degradation. Our findings highlight microbial activity as a primary driver of red stains in marble relics and underscore the importance of organic contaminant control in conservation.