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In March 2021, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by four families (Aliusviridae, Crepuscuviridae, Myriaviridae, and Natareviridae), three subfamilies (Alpharhabdovirinae, Betarhabdovirinae, and Gammarhabdovirinae), 42 genera, and 200 species. Thirty-nine species were renamed and/or moved and seven species were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
Jens H. Kuhn; Scott Adkins; Bernard R. Agwanda; Rim Al Kubrusli; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Christopher F. Basler; Sina Bavari; Martin Beer; Nicolas Bejerman; Andrew J. Bennett; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol D. Blair; Kim R. Blasdell; Dag-Ragnar Blystad; Jamie Bojko; Wayne B. Borth; Steven Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Judith K. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Carmen Büttner; Charles H. Calisher; Mengji Cao; Inmaculada Casas; Kartik Chandran; Rémi N. Charrel; Qi Cheng; Yuya Chiaki; Marco Chiapello; Il-Ryong Choi; Marina Ciuffo; J. Christopher S. Clegg; Ian Crozier; Elena Dal Bó; Juan Carlos de la Torre; Xavier de Lamballerie; Rik L. de Swart; Humberto Debat; Nolwenn M. Dheilly; Emiliano Di Cicco; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Olga Dolnik; Michael A. Drebot; J. Felix Drexler; William G. Dundon; W. Paul Duprex; Ralf Dürrwald; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Hugh W. Ferguson; Anthony R. Fooks; Marco Forgia; Pierre B. H. Formenty; Jana Fránová; Juliana Freitas-Astúa; Jingjing Fu; Stephanie Fürl; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Thomas Gaskin; Jean-Paul J. Gonzalez; Anthony Griffiths; Tony L. Goldberg; Martin H. Groschup; Stephan Günther; Roy A. Hall; John Hammond; Tong Han; Jussi Hepojoki; Roger Hewson; Jiang Hong; Ni Hong; Seiji Hongo; Masayuki Horie; John S. Hu; Tao Hu; Holly R. Hughes; Florian Hüttner; Timothy H. Hyndman; M. Ilyas; Risto Jalkanen; Dàohóng Jiāng; Gilda B. Jonson; Sandra Junglen; Fujio Kadono; Karia H. Kaukinen; Michael Kawate; Boris Klempa; Jonas Klingström; Gary Kobinger; Igor Koloniuk; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Kenji Kubota; Gael Kurath; Lies Laenen; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elliot J. Lefkowitz; Eric M. Leroy; Shaorong Li; Longhui Li; Jiànróng Lǐ; Huazhen Liu; Igor S. Lukashevich; Piet Maes; William Marciel de Souza; Marco Marklewitz; Sergio H. Marshall; Shin-Yi L. Marzano; Sebastien Massart; John W. McCauley; Michael Melzer; Nicole Mielke-Ehret; Kristina M. Miller; Tobi J. Ming; Ali Mirazimi; Gideon J. Mordecai; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Alejandro Olmedo-Velarde; Gustavo Palacios; Vicente Pallás; Bernadett Pályi; Anna Papa; Sofia Paraskevopoulou; Adam C. Park; Colin R. Parrish; David A. Patterson; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Carlotta Peracchio; Daniel R. Pérez; Thomas S. Postler; Liying Qi; Sheli R. Radoshitzky; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Gabriel Robles Luna; Víctor Romanowski; Paul Rota; Dennis Rubbenstroth; Luisa Rubino; Jonathan A. Runstadler; Sead Sabanadzovic; Amadou Alpha Sall; Maria S. Salvato; Rosemary Sang; Takahide Sasaya; Angela D. Schulze; Martin Schwemmle; Mang Shi; Xiǎohóng Shí; Zhènglì Shí; Yoshifumi Shimomoto; Yukio Shirako; Stuart G. Siddell; Peter Simmonds; Manuela Sironi; Guy Smagghe; Sophie Smither; Jin-Won Song; Kirsten Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Jari Sugano; Curtis A. Suttle; Amy Tabata; Ayato Takada; Shigeharu Takeuchi; David P. Tchouassi; Amy Teffer; Robert B. Tesh; Natalie J. Thornburg; Yasuhiro Tomitaka; Keizō Tomonaga; Noël Tordo; Baldwyn Torto; Jonathan S. Towner; Shinya Tsuda; Changchun Tu; Massimo Turina; Ioannis E. Tzanetakis; Janice Uchida; Tomio Usugi; Anna Maria Vaira; Marta Vallino; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Susanne von Bargen; Jiro Wada; Victoria Wahl; Peter J. Walker; Lin-Fa Wang; Guoping Wang; Yanxiang Wang; Yaqin Wang; Muhammad Waqas; Tàiyún Wèi; Shaohua Wen; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Jiangxiang Wu; Lei Xu; Hironobu Yanagisawa; Caixia Yang; Zuokun Yang; F. Murilo Zerbini; Lifeng Zhai; Yong-Zhen Zhang; Song Zhang; Jinguo Zhang; Zhe Zhang; Xueping Zhou. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology 2021, 1 -54.
AMA StyleJens H. Kuhn, Scott Adkins, Bernard R. Agwanda, Rim Al Kubrusli, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Tatjana Avšič-Županc, María A. Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Christopher F. Basler, Sina Bavari, Martin Beer, Nicolas Bejerman, Andrew J. Bennett, Dennis A. Bente, Éric Bergeron, Brian H. Bird, Carol D. Blair, Kim R. Blasdell, Dag-Ragnar Blystad, Jamie Bojko, Wayne B. Borth, Steven Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Judith K. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Carmen Büttner, Charles H. Calisher, Mengji Cao, Inmaculada Casas, Kartik Chandran, Rémi N. Charrel, Qi Cheng, Yuya Chiaki, Marco Chiapello, Il-Ryong Choi, Marina Ciuffo, J. Christopher S. Clegg, Ian Crozier, Elena Dal Bó, Juan Carlos de la Torre, Xavier de Lamballerie, Rik L. de Swart, Humberto Debat, Nolwenn M. Dheilly, Emiliano Di Cicco, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Olga Dolnik, Michael A. Drebot, J. Felix Drexler, William G. Dundon, W. Paul Duprex, Ralf Dürrwald, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Hugh W. Ferguson, Anthony R. Fooks, Marco Forgia, Pierre B. H. Formenty, Jana Fránová, Juliana Freitas-Astúa, Jingjing Fu, Stephanie Fürl, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R. Garrison, Thomas Gaskin, Jean-Paul J. Gonzalez, Anthony Griffiths, Tony L. Goldberg, Martin H. Groschup, Stephan Günther, Roy A. Hall, John Hammond, Tong Han, Jussi Hepojoki, Roger Hewson, Jiang Hong, Ni Hong, Seiji Hongo, Masayuki Horie, John S. Hu, Tao Hu, Holly R. Hughes, Florian Hüttner, Timothy H. Hyndman, M. Ilyas, Risto Jalkanen, Dàohóng Jiāng, Gilda B. Jonson, Sandra Junglen, Fujio Kadono, Karia H. Kaukinen, Michael Kawate, Boris Klempa, Jonas Klingström, Gary Kobinger, Igor Koloniuk, Hideki Kondō, Eugene V. Koonin, Mart Krupovic, Kenji Kubota, Gael Kurath, Lies Laenen, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elliot J. Lefkowitz, Eric M. Leroy, Shaorong Li, Longhui Li, Jiànróng Lǐ, Huazhen Liu, Igor S. Lukashevich, Piet Maes, William Marciel de Souza, Marco Marklewitz, Sergio H. Marshall, Shin-Yi L. Marzano, Sebastien Massart, John W. McCauley, Michael Melzer, Nicole Mielke-Ehret, Kristina M. Miller, Tobi J. Ming, Ali Mirazimi, Gideon J. Mordecai, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, José A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R. T. Nunes, Alejandro Olmedo-Velarde, Gustavo Palacios, Vicente Pallás, Bernadett Pályi, Anna Papa, Sofia Paraskevopoulou, Adam C. Park, Colin R. Parrish, David A. Patterson, Alex Pauvolid-Corrêa, Janusz T. Pawęska, Susan Payne, Carlotta Peracchio, Daniel R. Pérez, Thomas S. Postler, Liying Qi, Sheli R. Radoshitzky, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Gabriel Robles Luna, Víctor Romanowski, Paul Rota, Dennis Rubbenstroth, Luisa Rubino, Jonathan A. Runstadler, Sead Sabanadzovic, Amadou Alpha Sall, Maria S. Salvato, Rosemary Sang, Takahide Sasaya, Angela D. Schulze, Martin Schwemmle, Mang Shi, Xiǎohóng Shí, Zhènglì Shí, Yoshifumi Shimomoto, Yukio Shirako, Stuart G. Siddell, Peter Simmonds, Manuela Sironi, Guy Smagghe, Sophie Smither, Jin-Won Song, Kirsten Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Jari Sugano, Curtis A. Suttle, Amy Tabata, Ayato Takada, Shigeharu Takeuchi, David P. Tchouassi, Amy Teffer, Robert B. Tesh, Natalie J. Thornburg, Yasuhiro Tomitaka, Keizō Tomonaga, Noël Tordo, Baldwyn Torto, Jonathan S. Towner, Shinya Tsuda, Changchun Tu, Massimo Turina, Ioannis E. Tzanetakis, Janice Uchida, Tomio Usugi, Anna Maria Vaira, Marta Vallino, Bernadette Van Den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Susanne von Bargen, Jiro Wada, Victoria Wahl, Peter J. Walker, Lin-Fa Wang, Guoping Wang, Yanxiang Wang, Yaqin Wang, Muhammad Waqas, Tàiyún Wèi, Shaohua Wen, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Jiangxiang Wu, Lei Xu, Hironobu Yanagisawa, Caixia Yang, Zuokun Yang, F. Murilo Zerbini, Lifeng Zhai, Yong-Zhen Zhang, Song Zhang, Jinguo Zhang, Zhe Zhang, Xueping Zhou. 2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology. 2021; ():1-54.
Chicago/Turabian StyleJens H. Kuhn; Scott Adkins; Bernard R. Agwanda; Rim Al Kubrusli; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Christopher F. Basler; Sina Bavari; Martin Beer; Nicolas Bejerman; Andrew J. Bennett; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol D. Blair; Kim R. Blasdell; Dag-Ragnar Blystad; Jamie Bojko; Wayne B. Borth; Steven Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Judith K. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Carmen Büttner; Charles H. Calisher; Mengji Cao; Inmaculada Casas; Kartik Chandran; Rémi N. Charrel; Qi Cheng; Yuya Chiaki; Marco Chiapello; Il-Ryong Choi; Marina Ciuffo; J. Christopher S. Clegg; Ian Crozier; Elena Dal Bó; Juan Carlos de la Torre; Xavier de Lamballerie; Rik L. de Swart; Humberto Debat; Nolwenn M. Dheilly; Emiliano Di Cicco; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Olga Dolnik; Michael A. Drebot; J. Felix Drexler; William G. Dundon; W. Paul Duprex; Ralf Dürrwald; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Hugh W. Ferguson; Anthony R. Fooks; Marco Forgia; Pierre B. H. Formenty; Jana Fránová; Juliana Freitas-Astúa; Jingjing Fu; Stephanie Fürl; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Thomas Gaskin; Jean-Paul J. Gonzalez; Anthony Griffiths; Tony L. Goldberg; Martin H. Groschup; Stephan Günther; Roy A. Hall; John Hammond; Tong Han; Jussi Hepojoki; Roger Hewson; Jiang Hong; Ni Hong; Seiji Hongo; Masayuki Horie; John S. Hu; Tao Hu; Holly R. Hughes; Florian Hüttner; Timothy H. Hyndman; M. Ilyas; Risto Jalkanen; Dàohóng Jiāng; Gilda B. Jonson; Sandra Junglen; Fujio Kadono; Karia H. Kaukinen; Michael Kawate; Boris Klempa; Jonas Klingström; Gary Kobinger; Igor Koloniuk; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Kenji Kubota; Gael Kurath; Lies Laenen; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elliot J. Lefkowitz; Eric M. Leroy; Shaorong Li; Longhui Li; Jiànróng Lǐ; Huazhen Liu; Igor S. Lukashevich; Piet Maes; William Marciel de Souza; Marco Marklewitz; Sergio H. Marshall; Shin-Yi L. Marzano; Sebastien Massart; John W. McCauley; Michael Melzer; Nicole Mielke-Ehret; Kristina M. Miller; Tobi J. Ming; Ali Mirazimi; Gideon J. Mordecai; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Alejandro Olmedo-Velarde; Gustavo Palacios; Vicente Pallás; Bernadett Pályi; Anna Papa; Sofia Paraskevopoulou; Adam C. Park; Colin R. Parrish; David A. Patterson; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Carlotta Peracchio; Daniel R. Pérez; Thomas S. Postler; Liying Qi; Sheli R. Radoshitzky; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Gabriel Robles Luna; Víctor Romanowski; Paul Rota; Dennis Rubbenstroth; Luisa Rubino; Jonathan A. Runstadler; Sead Sabanadzovic; Amadou Alpha Sall; Maria S. Salvato; Rosemary Sang; Takahide Sasaya; Angela D. Schulze; Martin Schwemmle; Mang Shi; Xiǎohóng Shí; Zhènglì Shí; Yoshifumi Shimomoto; Yukio Shirako; Stuart G. Siddell; Peter Simmonds; Manuela Sironi; Guy Smagghe; Sophie Smither; Jin-Won Song; Kirsten Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Jari Sugano; Curtis A. Suttle; Amy Tabata; Ayato Takada; Shigeharu Takeuchi; David P. Tchouassi; Amy Teffer; Robert B. Tesh; Natalie J. Thornburg; Yasuhiro Tomitaka; Keizō Tomonaga; Noël Tordo; Baldwyn Torto; Jonathan S. Towner; Shinya Tsuda; Changchun Tu; Massimo Turina; Ioannis E. Tzanetakis; Janice Uchida; Tomio Usugi; Anna Maria Vaira; Marta Vallino; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Susanne von Bargen; Jiro Wada; Victoria Wahl; Peter J. Walker; Lin-Fa Wang; Guoping Wang; Yanxiang Wang; Yaqin Wang; Muhammad Waqas; Tàiyún Wèi; Shaohua Wen; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Jiangxiang Wu; Lei Xu; Hironobu Yanagisawa; Caixia Yang; Zuokun Yang; F. Murilo Zerbini; Lifeng Zhai; Yong-Zhen Zhang; Song Zhang; Jinguo Zhang; Zhe Zhang; Xueping Zhou. 2021. "2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales." Archives of Virology , no. : 1-54.
Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.
Yingyun Cai; Shuiqing Yu; Ying Fang; Laura Bollinger; Yanhua Li; Michael Lauck; Elena Postnikova; Steven Mazur; Reed Johnson; Courtney Finch; Sheli Radoshitzky; Gustavo Palacios; Thomas Friedrich; Tony Goldberg; David O’Connor; Peter Jahrling; Jens Kuhn. Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication. Viruses 2021, 13, 632 .
AMA StyleYingyun Cai, Shuiqing Yu, Ying Fang, Laura Bollinger, Yanhua Li, Michael Lauck, Elena Postnikova, Steven Mazur, Reed Johnson, Courtney Finch, Sheli Radoshitzky, Gustavo Palacios, Thomas Friedrich, Tony Goldberg, David O’Connor, Peter Jahrling, Jens Kuhn. Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication. Viruses. 2021; 13 (4):632.
Chicago/Turabian StyleYingyun Cai; Shuiqing Yu; Ying Fang; Laura Bollinger; Yanhua Li; Michael Lauck; Elena Postnikova; Steven Mazur; Reed Johnson; Courtney Finch; Sheli Radoshitzky; Gustavo Palacios; Thomas Friedrich; Tony Goldberg; David O’Connor; Peter Jahrling; Jens Kuhn. 2021. "Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication." Viruses 13, no. 4: 632.
In March 2020, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. At the genus rank, 20 new genera were added, two were deleted, one was moved, and three were renamed. At the species rank, 160 species were added, four were deleted, ten were moved and renamed, and 30 species were renamed. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.
Jens H. Kuhn; Scott Adkins; Daniela Alioto; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Simon J. Anthony; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Tomáš Bartonička; Christopher Basler; Sina Bavari; Martin Beer; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol Blair; Kim R. Blasdell; Steven B. Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Nihal Buzkan; Charles H. Calisher; Mengji Cao; Inmaculada Casas; John Chamberlain; Kartik Chandran; Rémi N. Charrel; Biao Chen; Michela Chiumenti; Il-Ryong Choi; J. Christopher S. Clegg; Ian Crozier; John V. Da Graça; Elena Dal Bó; Alberto M. R. Dávila; Juan Carlos De La Torre; Xavier De Lamballerie; Rik L. De Swart; Patrick L. Di Bello; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Valerian V. Dolja; Olga Dolnik; Michael A. Drebot; Jan Felix Drexler; Ralf Dürrwald; Lucie Dufkova; William G. Dundon; W. Paul Duprex; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Jorlan Fernandes; Anthony R. Fooks; Pierre B. H. Formenty; Leonie F. Forth; Ron A. M. Fouchier; Juliana Freitas-Astúa; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Aiah Gbakima; Tracey Goldstein; Jean-Paul J. Gonzalez; Anthony Griffiths; Martin H. Groschup; Stephan Günther; Alexandro Guterres; Roy A. Hall; John Hammond; Mohamed Hassan; Jussi Hepojoki; Satu Hepojoki; Udo Hetzel; Roger Hewson; Bernd Hoffmann; Seiji Hongo; Dirk Höper; Masayuki Horie; Holly R. Hughes; Timothy H. Hyndman; Amara Jambai; Rodrigo Jardim; Dàohóng Jiāng; Qi Jin; Gilda B. Jonson; Sandra Junglen; Serpil Karadağ; Karen E. Keller; Boris Klempa; Jonas Klingström; Gary Kobinger; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Gael Kurath; Ivan V. Kuzmin; Lies Laenen; Robert A. Lamb; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elba R. S. Lemos; Eric M. Leroy; Dexin Li; Jiànróng Lǐ; Mifang Liang; Wénwén Liú; Yàn Liú; Igor S. Lukashevich; Piet Maes; William Marciel De Souza; Marco Marklewitz; Sergio H. Marshall; Giovanni P. Martelli; Robert R. Martin; Shin-Yi L. Marzano; Sébastien Massart; John W. McCauley; Nicole Mielke-Ehret; Angelantonio Minafra; Maria Minutolo; Ali Mirazimi; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; Beatriz Navarro; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Are Nylund; Arnfinn L. Økland; Renata C. Oliveira; Gustavo Palacios; Vicente Pallas; Bernadett Pályi; Anna Papa; Colin R. Parrish; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Daniel R. Pérez; Florian Pfaff; Sheli R. Radoshitzky; Aziz-Ul Rahman; Pedro L. Ramos-González; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Víctor Romanowski; Gabriel Robles Luna; Paul Rota; Dennis Rubbenstroth; Jonathan A. Runstadler; Daniel Ruzek; Sead Sabanadzovic; Jiří Salát; Amadou Alpha Sall; Maria S. Salvato; Kamil Sarpkaya; Takahide Sasaya; Martin Schwemmle; Muhammad Z. Shabbir; Xiǎohóng Shí; Zhènglì Shí; Yukio Shirako; Peter Simmonds; Jana Širmarová; Manuela Sironi; Sophie Smither; Teemu Smura; Jin-Won Song; Kirsten M. Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Petra Straková; Ayato Takada; Robert B. Tesh; Natalie J. Thornburg; Keizō Tomonaga; Noël Tordo; Jonathan S. Towner; Massimo Turina; Ioannis Tzanetakis; Rainer G. Ulrich; Anna Maria Vaira; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Victoria Wahl; Peter J. Walker; Hui Wang; Jianwei Wang; Xifeng Wang; Lin-Fa Wang; Tàiyún Wèi; Heather Wells; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Zhìqiáng Wú; Xin Yang; Xīnglóu Yáng; Xuejie Yu; Natalya Yutin; F. Murilo Zerbini; Tong Zhang; Yong-Zhen Zhang; Guohui Zhou; Xueping Zhou. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology 2020, 165, 3023 -3072.
AMA StyleJens H. Kuhn, Scott Adkins, Daniela Alioto, Sergey V. Alkhovsky, Gaya K. Amarasinghe, Simon J. Anthony, Tatjana Avšič-Županc, María A. Ayllón, Justin Bahl, Anne Balkema-Buschmann, Matthew J. Ballinger, Tomáš Bartonička, Christopher Basler, Sina Bavari, Martin Beer, Dennis A. Bente, Éric Bergeron, Brian H. Bird, Carol Blair, Kim R. Blasdell, Steven B. Bradfute, Rachel Breyta, Thomas Briese, Paul A. Brown, Ursula J. Buchholz, Michael J. Buchmeier, Alexander Bukreyev, Felicity Burt, Nihal Buzkan, Charles H. Calisher, Mengji Cao, Inmaculada Casas, John Chamberlain, Kartik Chandran, Rémi N. Charrel, Biao Chen, Michela Chiumenti, Il-Ryong Choi, J. Christopher S. Clegg, Ian Crozier, John V. Da Graça, Elena Dal Bó, Alberto M. R. Dávila, Juan Carlos De La Torre, Xavier De Lamballerie, Rik L. De Swart, Patrick L. Di Bello, Nicholas Di Paola, Francesco Di Serio, Ralf G. Dietzgen, Michele Digiaro, Valerian V. Dolja, Olga Dolnik, Michael A. Drebot, Jan Felix Drexler, Ralf Dürrwald, Lucie Dufkova, William G. Dundon, W. Paul Duprex, John M. Dye, Andrew J. Easton, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Jorlan Fernandes, Anthony R. Fooks, Pierre B. H. Formenty, Leonie F. Forth, Ron A. M. Fouchier, Juliana Freitas-Astúa, Selma Gago-Zachert, George Fú Gāo, María Laura García, Adolfo García-Sastre, Aura R. Garrison, Aiah Gbakima, Tracey Goldstein, Jean-Paul J. Gonzalez, Anthony Griffiths, Martin H. Groschup, Stephan Günther, Alexandro Guterres, Roy A. Hall, John Hammond, Mohamed Hassan, Jussi Hepojoki, Satu Hepojoki, Udo Hetzel, Roger Hewson, Bernd Hoffmann, Seiji Hongo, Dirk Höper, Masayuki Horie, Holly R. Hughes, Timothy H. Hyndman, Amara Jambai, Rodrigo Jardim, Dàohóng Jiāng, Qi Jin, Gilda B. Jonson, Sandra Junglen, Serpil Karadağ, Karen E. Keller, Boris Klempa, Jonas Klingström, Gary Kobinger, Hideki Kondō, Eugene V. Koonin, Mart Krupovic, Gael Kurath, Ivan V. Kuzmin, Lies Laenen, Robert A. Lamb, Amy J. Lambert, Stanley L. Langevin, Benhur Lee, Elba R. S. Lemos, Eric M. Leroy, Dexin Li, Jiànróng Lǐ, Mifang Liang, Wénwén Liú, Yàn Liú, Igor S. Lukashevich, Piet Maes, William Marciel De Souza, Marco Marklewitz, Sergio H. Marshall, Giovanni P. Martelli, Robert R. Martin, Shin-Yi L. Marzano, Sébastien Massart, John W. McCauley, Nicole Mielke-Ehret, Angelantonio Minafra, Maria Minutolo, Ali Mirazimi, Hans-Peter Mühlbach, Elke Mühlberger, Rayapati Naidu, Tomohide Natsuaki, Beatriz Navarro, José A. Navarro, Sergey V. Netesov, Gabriele Neumann, Norbert Nowotny, Márcio R. T. Nunes, Are Nylund, Arnfinn L. Økland, Renata C. Oliveira, Gustavo Palacios, Vicente Pallas, Bernadett Pályi, Anna Papa, Colin R. Parrish, Alex Pauvolid-Corrêa, Janusz T. Pawęska, Susan Payne, Daniel R. Pérez, Florian Pfaff, Sheli R. Radoshitzky, Aziz-Ul Rahman, Pedro L. Ramos-González, Renato O. Resende, Carina A. Reyes, Bertus K. Rima, Víctor Romanowski, Gabriel Robles Luna, Paul Rota, Dennis Rubbenstroth, Jonathan A. Runstadler, Daniel Ruzek, Sead Sabanadzovic, Jiří Salát, Amadou Alpha Sall, Maria S. Salvato, Kamil Sarpkaya, Takahide Sasaya, Martin Schwemmle, Muhammad Z. Shabbir, Xiǎohóng Shí, Zhènglì Shí, Yukio Shirako, Peter Simmonds, Jana Širmarová, Manuela Sironi, Sophie Smither, Teemu Smura, Jin-Won Song, Kirsten M. Spann, Jessica R. Spengler, Mark D. Stenglein, David M. Stone, Petra Straková, Ayato Takada, Robert B. Tesh, Natalie J. Thornburg, Keizō Tomonaga, Noël Tordo, Jonathan S. Towner, Massimo Turina, Ioannis Tzanetakis, Rainer G. Ulrich, Anna Maria Vaira, Bernadette Van Den Hoogen, Arvind Varsani, Nikos Vasilakis, Martin Verbeek, Victoria Wahl, Peter J. Walker, Hui Wang, Jianwei Wang, Xifeng Wang, Lin-Fa Wang, Tàiyún Wèi, Heather Wells, Anna E. Whitfield, John V. Williams, Yuri I. Wolf, Zhìqiáng Wú, Xin Yang, Xīnglóu Yáng, Xuejie Yu, Natalya Yutin, F. Murilo Zerbini, Tong Zhang, Yong-Zhen Zhang, Guohui Zhou, Xueping Zhou. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Archives of Virology. 2020; 165 (12):3023-3072.
Chicago/Turabian StyleJens H. Kuhn; Scott Adkins; Daniela Alioto; Sergey V. Alkhovsky; Gaya K. Amarasinghe; Simon J. Anthony; Tatjana Avšič-Županc; María A. Ayllón; Justin Bahl; Anne Balkema-Buschmann; Matthew J. Ballinger; Tomáš Bartonička; Christopher Basler; Sina Bavari; Martin Beer; Dennis A. Bente; Éric Bergeron; Brian H. Bird; Carol Blair; Kim R. Blasdell; Steven B. Bradfute; Rachel Breyta; Thomas Briese; Paul A. Brown; Ursula J. Buchholz; Michael J. Buchmeier; Alexander Bukreyev; Felicity Burt; Nihal Buzkan; Charles H. Calisher; Mengji Cao; Inmaculada Casas; John Chamberlain; Kartik Chandran; Rémi N. Charrel; Biao Chen; Michela Chiumenti; Il-Ryong Choi; J. Christopher S. Clegg; Ian Crozier; John V. Da Graça; Elena Dal Bó; Alberto M. R. Dávila; Juan Carlos De La Torre; Xavier De Lamballerie; Rik L. De Swart; Patrick L. Di Bello; Nicholas Di Paola; Francesco Di Serio; Ralf G. Dietzgen; Michele Digiaro; Valerian V. Dolja; Olga Dolnik; Michael A. Drebot; Jan Felix Drexler; Ralf Dürrwald; Lucie Dufkova; William G. Dundon; W. Paul Duprex; John M. Dye; Andrew J. Easton; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Jorlan Fernandes; Anthony R. Fooks; Pierre B. H. Formenty; Leonie F. Forth; Ron A. M. Fouchier; Juliana Freitas-Astúa; Selma Gago-Zachert; George Fú Gāo; María Laura García; Adolfo García-Sastre; Aura R. Garrison; Aiah Gbakima; Tracey Goldstein; Jean-Paul J. Gonzalez; Anthony Griffiths; Martin H. Groschup; Stephan Günther; Alexandro Guterres; Roy A. Hall; John Hammond; Mohamed Hassan; Jussi Hepojoki; Satu Hepojoki; Udo Hetzel; Roger Hewson; Bernd Hoffmann; Seiji Hongo; Dirk Höper; Masayuki Horie; Holly R. Hughes; Timothy H. Hyndman; Amara Jambai; Rodrigo Jardim; Dàohóng Jiāng; Qi Jin; Gilda B. Jonson; Sandra Junglen; Serpil Karadağ; Karen E. Keller; Boris Klempa; Jonas Klingström; Gary Kobinger; Hideki Kondō; Eugene V. Koonin; Mart Krupovic; Gael Kurath; Ivan V. Kuzmin; Lies Laenen; Robert A. Lamb; Amy J. Lambert; Stanley L. Langevin; Benhur Lee; Elba R. S. Lemos; Eric M. Leroy; Dexin Li; Jiànróng Lǐ; Mifang Liang; Wénwén Liú; Yàn Liú; Igor S. Lukashevich; Piet Maes; William Marciel De Souza; Marco Marklewitz; Sergio H. Marshall; Giovanni P. Martelli; Robert R. Martin; Shin-Yi L. Marzano; Sébastien Massart; John W. McCauley; Nicole Mielke-Ehret; Angelantonio Minafra; Maria Minutolo; Ali Mirazimi; Hans-Peter Mühlbach; Elke Mühlberger; Rayapati Naidu; Tomohide Natsuaki; Beatriz Navarro; José A. Navarro; Sergey V. Netesov; Gabriele Neumann; Norbert Nowotny; Márcio R. T. Nunes; Are Nylund; Arnfinn L. Økland; Renata C. Oliveira; Gustavo Palacios; Vicente Pallas; Bernadett Pályi; Anna Papa; Colin R. Parrish; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Susan Payne; Daniel R. Pérez; Florian Pfaff; Sheli R. Radoshitzky; Aziz-Ul Rahman; Pedro L. Ramos-González; Renato O. Resende; Carina A. Reyes; Bertus K. Rima; Víctor Romanowski; Gabriel Robles Luna; Paul Rota; Dennis Rubbenstroth; Jonathan A. Runstadler; Daniel Ruzek; Sead Sabanadzovic; Jiří Salát; Amadou Alpha Sall; Maria S. Salvato; Kamil Sarpkaya; Takahide Sasaya; Martin Schwemmle; Muhammad Z. Shabbir; Xiǎohóng Shí; Zhènglì Shí; Yukio Shirako; Peter Simmonds; Jana Širmarová; Manuela Sironi; Sophie Smither; Teemu Smura; Jin-Won Song; Kirsten M. Spann; Jessica R. Spengler; Mark D. Stenglein; David M. Stone; Petra Straková; Ayato Takada; Robert B. Tesh; Natalie J. Thornburg; Keizō Tomonaga; Noël Tordo; Jonathan S. Towner; Massimo Turina; Ioannis Tzanetakis; Rainer G. Ulrich; Anna Maria Vaira; Bernadette Van Den Hoogen; Arvind Varsani; Nikos Vasilakis; Martin Verbeek; Victoria Wahl; Peter J. Walker; Hui Wang; Jianwei Wang; Xifeng Wang; Lin-Fa Wang; Tàiyún Wèi; Heather Wells; Anna E. Whitfield; John V. Williams; Yuri I. Wolf; Zhìqiáng Wú; Xin Yang; Xīnglóu Yáng; Xuejie Yu; Natalya Yutin; F. Murilo Zerbini; Tong Zhang; Yong-Zhen Zhang; Guohui Zhou; Xueping Zhou. 2020. "2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales." Archives of Virology 165, no. 12: 3023-3072.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of human coronavirus disease 2019 (COVID-19), emerged in Wuhan, China, in December 2019. The virus rapidly spread globally, resulting in a public health crisis including almost 5 million cases and 323,256 deaths as of May 21, 2020. Here, we describe the identification and evaluation of commercially available reagents and assays for the molecular detection of SARS-CoV-2 in infected FFPE cell pellets. We identified a suitable rabbit polyclonal anti–SARS-CoV spike protein antibody and a mouse monoclonal anti–SARS-CoV nucleocapsid protein (NP) antibody for cross-detection of the respective SARS-CoV-2 proteins by IHC and immunofluorescence assay (IFA). Next, we established RNAscope in situ hybridization (ISH) to detect SARS-CoV-2 RNA. Furthermore, we established a multiplex FISH (mFISH) to detect positive-sense SARS-CoV-2 RNA and negative-sense SARS-CoV-2 RNA (a replicative intermediate indicating viral replication). Finally, we developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 antigen and RNA in the same FFPE section. It is hoped that these reagents and assays will accelerate COVID-19 pathogenesis studies in humans and in COVID-19 animal models.
Jun Liu; April M. Babka; Brian J. Kearney; Sheli R. Radoshitzky; Jens H. Kuhn; Xiankun Zeng. Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens. JCI Insight 2020, 5, 1 .
AMA StyleJun Liu, April M. Babka, Brian J. Kearney, Sheli R. Radoshitzky, Jens H. Kuhn, Xiankun Zeng. Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens. JCI Insight. 2020; 5 (12):1.
Chicago/Turabian StyleJun Liu; April M. Babka; Brian J. Kearney; Sheli R. Radoshitzky; Jens H. Kuhn; Xiankun Zeng. 2020. "Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens." JCI Insight 5, no. 12: 1.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of human coronavirus disease 2019 (COVID-19), emerged in Wuhan, China in December 2019. The virus rapidly spread globally, resulting in a public-health crisis including more than one million cases and tens of thousands of deaths. Here, we describe the identification and evaluation of commercially available reagents and assays for the molecular detection of SARS-CoV-2 in infected formalin fixed paraffin embedded (FFPE) cell pellets. We identified a suitable rabbit polyclonal anti-SARS-CoV spike protein antibody and a mouse monoclonal anti-SARS-CoV nucleocapsid protein (NP) antibody for cross detection of the respective SARS-CoV-2 proteins by immunohistochemistry (IHC) and immunofluorescence assay (IFA). Next, we established RNAscopein situhybridization (ISH) to detect SARS-CoV-2 RNA. Furthermore, we established a multiplex fluorescence ISH (mFISH) to detect positive-sense SARS-CoV-2 RNA and negative-sense SARS-CoV-2 RNA (a replicative intermediate indicating viral replication). Finally, we developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 antigen and RNA in the same FFPE section. These reagents and assays will accelerate COVID-19 pathogenesis studies in humans and in COVID-19 animal models.
Jun Liu; April M. Babka; Brian J. Kearney; Sheli R. Radoshitzky; Jens H. Kuhn; Xiankun Zeng. Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens. 2020, 1 .
AMA StyleJun Liu, April M. Babka, Brian J. Kearney, Sheli R. Radoshitzky, Jens H. Kuhn, Xiankun Zeng. Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens. . 2020; ():1.
Chicago/Turabian StyleJun Liu; April M. Babka; Brian J. Kearney; Sheli R. Radoshitzky; Jens H. Kuhn; Xiankun Zeng. 2020. "Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens." , no. : 1.
Summary All viruses balance interactions between cellular machinery co-opted to support replication and host factors deployed to halt the infection. We use gene correlation analysis to perform an unbiased screen for host factors involved in influenza A virus (FLUAV) infection. Our screen identifies the cellular factor epidermal growth factor receptor pathway substrate 8 (EPS8) as the highest confidence pro-viral candidate. Knockout and overexpression of EPS8 confirm its importance in enhancing FLUAV infection and titers. Loss of EPS8 does not affect virion attachment, uptake, or fusion. Rather, our data show that EPS8 specifically functions during virion uncoating. EPS8 physically associates with incoming virion components, and subsequent nuclear import of released ribonucleoprotein complexes is significantly delayed in the absence of EPS8. Our study identifies EPS8 as a host factor important for uncoating, a crucial step of FLUAV infection during which the interface between the virus and host is still being discovered.
Gloria P. Larson; Vy Tran; Shuǐqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle. EPS8 Facilitates Uncoating of Influenza A Virus. Cell Reports 2019, 29, 2175 -2183.e4.
AMA StyleGloria P. Larson, Vy Tran, Shuǐqìng Yú, Yíngyún Caì, Christina A. Higgins, Danielle M. Smith, Steven F. Baker, Sheli R. Radoshitzky, Jens H. Kuhn, Andrew Mehle. EPS8 Facilitates Uncoating of Influenza A Virus. Cell Reports. 2019; 29 (8):2175-2183.e4.
Chicago/Turabian StyleGloria P. Larson; Vy Tran; Shuǐqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle. 2019. "EPS8 Facilitates Uncoating of Influenza A Virus." Cell Reports 29, no. 8: 2175-2183.e4.
In February 2019, following the annual taxon ratification vote, the order Bunyavirales was amended by creation of two new families, four new subfamilies, 11 new genera and 77 new species, merging of two species, and deletion of one species. This article presents the updated taxonomy of the order Bunyavirales now accepted by the International Committee on Taxonomy of Viruses (ICTV).
Abulikemu Abudurexiti; Scott Adkins; Daniela Alioto; Sergey V. Alkhovsky; Tatjana Avšič-Županc; Matthew J. Ballinger; Dennis A. Bente; Martin Beer; Éric Bergeron; Carol D. Blair; Thomas Briese; Michael J. Buchmeier; Felicity J. Burt; Charles H. Calisher; Chénchén Cháng; Rémi N. Charrel; Il Ryong Choi; J. Christopher S. Clegg; Juan Carlos De La Torre; Xavier De Lamballerie; Fēi Dèng; Francesco Di Serio; Michele Digiaro; Michael A. Drebot; Xiǎoméi Duàn; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Charles F. Fulhorst; Aura R. Garrison; George Fú Gāo; Jean-Paul J. Gonzalez; Martin H. Groschup; Stephan Günther; Anne-Lise Haenni; Roy A. Hall; Jussi Hepojoki; Roger Hewson; Zhìhóng Hú; Holly R. Hughes; Miranda Gilda Jonson; Sandra Junglen; Boris Klempa; Jonas Klingström; Chūn Kòu; Lies Laenen; Amy J. Lambert; Stanley A. Langevin; Dan Liu; Igor S. Lukashevich; Tāo Luò; Chuánwèi Lǚ; Piet Maes; William Marciel De Souza; Marco Marklewitz; Giovanni P. Martelli; Keita Matsuno; Nicole Mielke-Ehret; Maria Minutolo; Ali Mirazimi; Abulimiti Moming; Hans-Peter Mühlbach; Rayapati Naidu; Beatriz Navarro; Márcio Roberto Teixeira Nunes; Gustavo Palacios; Anna Papa; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Jié Qiáo; Sheli R. Radoshitzky; Renato O. Resende; Víctor Romanowski; Amadou Alpha Sall; Maria S. Salvato; Takahide Sasaya; Shū Shěn; Xiǎohóng Shí; Yukio Shirako; Peter Simmonds; Manuela Sironi; Jin-Won Song; Jessica R. Spengler; Mark D. Stenglein; Zhèngyuán Sū; Sùróng Sūn; Shuāng Táng; Massimo Turina; Bó Wáng; Chéng Wáng; Huálín Wáng; Jūn Wáng; Tàiyún Wèi; Anna E. Whitfield; F. Murilo Zerbini; Jìngyuàn Zhāng; Lěi Zhāng; Yànfāng Zhāng; Yong-Zhen Zhang; Yújiāng Zhāng; Xueping Zhou; Lìyǐng Zhū; Jens H. Kuhn. Taxonomy of the order Bunyavirales: update 2019. Archives of Virology 2019, 164, 1949 -1965.
AMA StyleAbulikemu Abudurexiti, Scott Adkins, Daniela Alioto, Sergey V. Alkhovsky, Tatjana Avšič-Županc, Matthew J. Ballinger, Dennis A. Bente, Martin Beer, Éric Bergeron, Carol D. Blair, Thomas Briese, Michael J. Buchmeier, Felicity J. Burt, Charles H. Calisher, Chénchén Cháng, Rémi N. Charrel, Il Ryong Choi, J. Christopher S. Clegg, Juan Carlos De La Torre, Xavier De Lamballerie, Fēi Dèng, Francesco Di Serio, Michele Digiaro, Michael A. Drebot, Xiǎoméi Duàn, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Charles F. Fulhorst, Aura R. Garrison, George Fú Gāo, Jean-Paul J. Gonzalez, Martin H. Groschup, Stephan Günther, Anne-Lise Haenni, Roy A. Hall, Jussi Hepojoki, Roger Hewson, Zhìhóng Hú, Holly R. Hughes, Miranda Gilda Jonson, Sandra Junglen, Boris Klempa, Jonas Klingström, Chūn Kòu, Lies Laenen, Amy J. Lambert, Stanley A. Langevin, Dan Liu, Igor S. Lukashevich, Tāo Luò, Chuánwèi Lǚ, Piet Maes, William Marciel De Souza, Marco Marklewitz, Giovanni P. Martelli, Keita Matsuno, Nicole Mielke-Ehret, Maria Minutolo, Ali Mirazimi, Abulimiti Moming, Hans-Peter Mühlbach, Rayapati Naidu, Beatriz Navarro, Márcio Roberto Teixeira Nunes, Gustavo Palacios, Anna Papa, Alex Pauvolid-Corrêa, Janusz T. Pawęska, Jié Qiáo, Sheli R. Radoshitzky, Renato O. Resende, Víctor Romanowski, Amadou Alpha Sall, Maria S. Salvato, Takahide Sasaya, Shū Shěn, Xiǎohóng Shí, Yukio Shirako, Peter Simmonds, Manuela Sironi, Jin-Won Song, Jessica R. Spengler, Mark D. Stenglein, Zhèngyuán Sū, Sùróng Sūn, Shuāng Táng, Massimo Turina, Bó Wáng, Chéng Wáng, Huálín Wáng, Jūn Wáng, Tàiyún Wèi, Anna E. Whitfield, F. Murilo Zerbini, Jìngyuàn Zhāng, Lěi Zhāng, Yànfāng Zhāng, Yong-Zhen Zhang, Yújiāng Zhāng, Xueping Zhou, Lìyǐng Zhū, Jens H. Kuhn. Taxonomy of the order Bunyavirales: update 2019. Archives of Virology. 2019; 164 (7):1949-1965.
Chicago/Turabian StyleAbulikemu Abudurexiti; Scott Adkins; Daniela Alioto; Sergey V. Alkhovsky; Tatjana Avšič-Županc; Matthew J. Ballinger; Dennis A. Bente; Martin Beer; Éric Bergeron; Carol D. Blair; Thomas Briese; Michael J. Buchmeier; Felicity J. Burt; Charles H. Calisher; Chénchén Cháng; Rémi N. Charrel; Il Ryong Choi; J. Christopher S. Clegg; Juan Carlos De La Torre; Xavier De Lamballerie; Fēi Dèng; Francesco Di Serio; Michele Digiaro; Michael A. Drebot; Xiǎoméi Duàn; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Charles F. Fulhorst; Aura R. Garrison; George Fú Gāo; Jean-Paul J. Gonzalez; Martin H. Groschup; Stephan Günther; Anne-Lise Haenni; Roy A. Hall; Jussi Hepojoki; Roger Hewson; Zhìhóng Hú; Holly R. Hughes; Miranda Gilda Jonson; Sandra Junglen; Boris Klempa; Jonas Klingström; Chūn Kòu; Lies Laenen; Amy J. Lambert; Stanley A. Langevin; Dan Liu; Igor S. Lukashevich; Tāo Luò; Chuánwèi Lǚ; Piet Maes; William Marciel De Souza; Marco Marklewitz; Giovanni P. Martelli; Keita Matsuno; Nicole Mielke-Ehret; Maria Minutolo; Ali Mirazimi; Abulimiti Moming; Hans-Peter Mühlbach; Rayapati Naidu; Beatriz Navarro; Márcio Roberto Teixeira Nunes; Gustavo Palacios; Anna Papa; Alex Pauvolid-Corrêa; Janusz T. Pawęska; Jié Qiáo; Sheli R. Radoshitzky; Renato O. Resende; Víctor Romanowski; Amadou Alpha Sall; Maria S. Salvato; Takahide Sasaya; Shū Shěn; Xiǎohóng Shí; Yukio Shirako; Peter Simmonds; Manuela Sironi; Jin-Won Song; Jessica R. Spengler; Mark D. Stenglein; Zhèngyuán Sū; Sùróng Sūn; Shuāng Táng; Massimo Turina; Bó Wáng; Chéng Wáng; Huálín Wáng; Jūn Wáng; Tàiyún Wèi; Anna E. Whitfield; F. Murilo Zerbini; Jìngyuàn Zhāng; Lěi Zhāng; Yànfāng Zhāng; Yong-Zhen Zhang; Yújiāng Zhāng; Xueping Zhou; Lìyǐng Zhū; Jens H. Kuhn. 2019. "Taxonomy of the order Bunyavirales: update 2019." Archives of Virology 164, no. 7: 1949-1965.
In 2012, the genome of a novel rhabdovirus, Bas-Congo virus (BASV), was discovered in the acute-phase serum of a Congolese patient with presumed viral hemorrhagic fever. In the absence of a replicating virus isolate, fulfilling Koch’s postulates to determine whether BASV is indeed a human virus and/or pathogen has been impossible. However, experiments with vesiculoviral particles pseudotyped with Bas-Congo glycoprotein suggested that BASV particles can enter cells from multiple animals, including humans. In 2015, genomes of two related viruses, Ekpoma virus 1 (EKV-1) and Ekpoma virus 2 (EKV-2), were detected in human sera in Nigeria. Isolates could not be obtained. Phylogenetic analyses led to the classification of BASV, EKV-1, and EKV-2 in the same genus, Tibrovirus, together with five biting midge-borne rhabdoviruses [i.e., Beatrice Hill virus (BHV), Bivens Arm virus (BAV), Coastal Plains virus (CPV), Sweetwater Branch virus (SWBV), and Tibrogargan virus (TIBV)] not known to infect humans. Using individual recombinant vesiculoviruses expressing the glycoproteins of all eight known tibroviruses and more than 75 cell lines representing different animal species, we demonstrate that the glycoproteins of all tibroviruses can mediate vesiculovirus particle entry into human, bat, nonhuman primate, cotton rat, boa constrictor, and Asian tiger mosquito cells. Using four of five isolated authentic tibroviruses (i.e., BAV, CPV, SWBV, and TIBV), our experiments indicate that many cell types may be partially resistant to tibrovirus replication after virion cell entry. Consequently, experimental data solely obtained from experiments using tibrovirus surrogate systems (e.g., vesiculoviral pseudotypes, recombinant vesiculoviruses) cannot be used to predict whether BASV, or any other tibrovirus, infects humans.
Yíngyún Caì; Shuǐqìng Yú; Rohit K. Jangra; Elena N. Postnikova; Jiro Wada; Robert B. Tesh; Sean P. J. Whelan; Michael Lauck; Michael R. Wiley; Courtney L. Finch; Sheli R. Radoshitzky; David H. O’Connor; Gustavo Palacios; Kartik Chandran; Charles Y. Chiu; Jens H. Kuhn. Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry. Frontiers in Microbiology 2019, 10, 856 .
AMA StyleYíngyún Caì, Shuǐqìng Yú, Rohit K. Jangra, Elena N. Postnikova, Jiro Wada, Robert B. Tesh, Sean P. J. Whelan, Michael Lauck, Michael R. Wiley, Courtney L. Finch, Sheli R. Radoshitzky, David H. O’Connor, Gustavo Palacios, Kartik Chandran, Charles Y. Chiu, Jens H. Kuhn. Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry. Frontiers in Microbiology. 2019; 10 ():856.
Chicago/Turabian StyleYíngyún Caì; Shuǐqìng Yú; Rohit K. Jangra; Elena N. Postnikova; Jiro Wada; Robert B. Tesh; Sean P. J. Whelan; Michael Lauck; Michael R. Wiley; Courtney L. Finch; Sheli R. Radoshitzky; David H. O’Connor; Gustavo Palacios; Kartik Chandran; Charles Y. Chiu; Jens H. Kuhn. 2019. "Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry." Frontiers in Microbiology 10, no. : 856.
SUMMARYAll viruses balance interactions between cellular machinery co-opted to support replication and host factors deployed to halt the infection. We used gene correlation analysis to perform an unbiased screen for host factors involved in influenza A virus (FLUAV) infection. Our screen identified the cellular factor epidermal growth factor receptor pathway substrate 8 (EPS8) as the highest confidence pro-viral candidate. Knockout and overexpression of EPS8 confirmed its importance in enhancing FLUAV infection and titers. Loss of EPS8 did not affect virion attachment, uptake, or fusion. Rather, our data show that EPS8 specifically functions during virion uncoating. EPS8 physically associated with incoming virion components, and subsequent nuclear import of released ribonucleoprotein complexes was significantly delayed in the absence of EPS8. Our study identified EPS8 as a host factor important for uncoating, a crucial step of FLUAV infection during which the interface between the virus and host is still being discovered.
Gloria P. Larson; Vy Tran; Shuǐqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle; Shuiqìng Yú. EPS8 facilitates uncoating of influenza A virus. 2019, 592485 .
AMA StyleGloria P. Larson, Vy Tran, Shuǐqìng Yú, Yíngyún Caì, Christina A. Higgins, Danielle M. Smith, Steven F. Baker, Sheli R. Radoshitzky, Jens H. Kuhn, Andrew Mehle, Shuiqìng Yú. EPS8 facilitates uncoating of influenza A virus. . 2019; ():592485.
Chicago/Turabian StyleGloria P. Larson; Vy Tran; Shuǐqìng Yú; Yíngyún Caì; Christina A. Higgins; Danielle M. Smith; Steven F. Baker; Sheli R. Radoshitzky; Jens H. Kuhn; Andrew Mehle; Shuiqìng Yú. 2019. "EPS8 facilitates uncoating of influenza A virus." , no. : 592485.
The International Committee on Taxonomy of Viruses (ICTV) is tasked with classifying viruses into taxa (phyla to species) and devising taxon names. Virus names and virus name abbreviations are currently not within the ICTV’s official remit and are not regulated by an official entity. Many scientists, medical/veterinary professionals, and regulatory agencies do not address evolutionary questions nor are they concerned with the hierarchical organization of the viral world, and therefore, have limited use for ICTV-devised taxa. Instead, these professionals look to the ICTV as an expert point source that provides the most current taxonomic affiliations of viruses of interests to facilitate document writing. These needs are currently unmet as an ICTV-supported, easily searchable database that includes all published virus names and abbreviations linked to their taxa is not available. In addition, in stark contrast to other biological taxonomic frameworks, virus taxonomy currently permits individual species to have several members. Consequently, confusion emerges among those who are not aware of the difference between taxa and viruses, and because certain well-known viruses cannot be located in ICTV publications or be linked to their species. In addition, the number of duplicate names and abbreviations has increased dramatically in the literature. To solve this conundrum, the ICTV could mandate listing all viruses of established species and all reported unclassified viruses in forthcoming online ICTV Reports and create a searchable webpage using this information. The International Union of Microbiology Societies could also consider changing the mandate of the ICTV to include the nomenclature of all viruses in addition to taxon considerations. With such a mandate expansion, official virus names and virus name abbreviations could be catalogued and virus nomenclature could be standardized. As a result, the ICTV would become an even more useful resource for all stakeholders in virology.
Charles H Calisher; Thomas Briese; J Rodney Brister; Rémi N Charrel; Ralf Dürrwald; Hideki Ebihara; Charles F Fulhorst; George Fú Gāo; Martin H Groschup; Andrew D Haddow; Timothy H Hyndman; Sandra Junglen; Boris Klempa; Jonas Klingstrom; Andrew M Kropinski; Mart Krupovic; A Desiree LaBeaud; Piet Maes; Norbert Nowotny; Márcio Roberto Teixeira Nunes; Susan L Payne; Sheli Radoshitzky; Dennis Rubbenstroth; Sead Sabanadzovic; Takahide Sasaya; Mark D Stenglein; Arvind Varsani; Victoria Wahl; Scott C Weaver; Francisco Murilo Zerbini; Nikos Vasilakis; Jens H Kuhn. Strengthening the Interaction of the Virology Community with the International Committee on Taxonomy of Viruses (ICTV) by Linking Virus Names and Their Abbreviations to Virus Species. Systematic Biology 2019, 68, 828 -839.
AMA StyleCharles H Calisher, Thomas Briese, J Rodney Brister, Rémi N Charrel, Ralf Dürrwald, Hideki Ebihara, Charles F Fulhorst, George Fú Gāo, Martin H Groschup, Andrew D Haddow, Timothy H Hyndman, Sandra Junglen, Boris Klempa, Jonas Klingstrom, Andrew M Kropinski, Mart Krupovic, A Desiree LaBeaud, Piet Maes, Norbert Nowotny, Márcio Roberto Teixeira Nunes, Susan L Payne, Sheli Radoshitzky, Dennis Rubbenstroth, Sead Sabanadzovic, Takahide Sasaya, Mark D Stenglein, Arvind Varsani, Victoria Wahl, Scott C Weaver, Francisco Murilo Zerbini, Nikos Vasilakis, Jens H Kuhn. Strengthening the Interaction of the Virology Community with the International Committee on Taxonomy of Viruses (ICTV) by Linking Virus Names and Their Abbreviations to Virus Species. Systematic Biology. 2019; 68 (5):828-839.
Chicago/Turabian StyleCharles H Calisher; Thomas Briese; J Rodney Brister; Rémi N Charrel; Ralf Dürrwald; Hideki Ebihara; Charles F Fulhorst; George Fú Gāo; Martin H Groschup; Andrew D Haddow; Timothy H Hyndman; Sandra Junglen; Boris Klempa; Jonas Klingstrom; Andrew M Kropinski; Mart Krupovic; A Desiree LaBeaud; Piet Maes; Norbert Nowotny; Márcio Roberto Teixeira Nunes; Susan L Payne; Sheli Radoshitzky; Dennis Rubbenstroth; Sead Sabanadzovic; Takahide Sasaya; Mark D Stenglein; Arvind Varsani; Victoria Wahl; Scott C Weaver; Francisco Murilo Zerbini; Nikos Vasilakis; Jens H Kuhn. 2019. "Strengthening the Interaction of the Virology Community with the International Committee on Taxonomy of Viruses (ICTV) by Linking Virus Names and Their Abbreviations to Virus Species." Systematic Biology 68, no. 5: 828-839.
In October 2018, the order Bunyavirales was amended by inclusion of the family Arenaviridae, abolishment of three families, creation of three new families, 19 new genera, and 14 new species, and renaming of three genera and 22 species. This article presents the updated taxonomy of the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV).
Piet Maes; Scott Adkins; Sergey V. Alkhovsky; Tatjana Avšič Županc; Matthew J. Ballinger; Dennis A. Bente; Martin Beer; Éric Bergeron; Carol D. Blair; Thomas Briese; Michael J. Buchmeier; Felicity J. Burt; Charles H. Calisher; Rémi N. Charrel; Il Ryong Choi; J. Christopher S. Clegg; Juan Carlos De La Torre; Xavier De Lamballerie; Joseph L. DeRisi; Michele Digiaro; Mike Drebot; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Charles F. Fulhorst; Aura R. Garrison; George Fú Gāo; Jean-Paul J. Gonzalez; Martin H. Groschup; Stephan Günther; Anne-Lise Haenni; Roy A. Hall; Roger Hewson; Holly R. Hughes; Rakesh K. Jain; Miranda Gilda Jonson; Sandra Junglen; Boris Klempa; Jonas Klingström; Richard Kormelink; Amy J. Lambert; Stanley A. Langevin; Igor S. Lukashevich; Marco Marklewitz; Giovanni P. Martelli; Nicole Mielke-Ehret; Ali Mirazimi; Hans-Peter Mühlbach; Rayapati Naidu; Márcio Roberto Teixeira Nunes; Gustavo Palacios; Anna Papa; Janusz T. Pawęska; Clarence J. Peters; Alexander Plyusnin; Sheli R. Radoshitzky; Renato O. Resende; Victor Romanowski; Amadou Alpha Sall; Maria S. Salvato; Takahide Sasaya; Connie Schmaljohn; Xiǎohóng Shí; Yukio Shirako; Peter Simmonds; Manuela Sironi; Jin-Won Song; Jessica R. Spengler; Mark D. Stenglein; Robert B. Tesh; Massimo Turina; Tàiyún Wèi; Anna E. Whitfield; Shyi-Dong Yeh; F. Murilo Zerbini; Yong-Zhen Zhang; Xueping Zhou; Jens H. Kuhn. Taxonomy of the order Bunyavirales: second update 2018. Archives of Virology 2019, 164, 927 -941.
AMA StylePiet Maes, Scott Adkins, Sergey V. Alkhovsky, Tatjana Avšič Županc, Matthew J. Ballinger, Dennis A. Bente, Martin Beer, Éric Bergeron, Carol D. Blair, Thomas Briese, Michael J. Buchmeier, Felicity J. Burt, Charles H. Calisher, Rémi N. Charrel, Il Ryong Choi, J. Christopher S. Clegg, Juan Carlos De La Torre, Xavier De Lamballerie, Joseph L. DeRisi, Michele Digiaro, Mike Drebot, Hideki Ebihara, Toufic Elbeaino, Koray Ergünay, Charles F. Fulhorst, Aura R. Garrison, George Fú Gāo, Jean-Paul J. Gonzalez, Martin H. Groschup, Stephan Günther, Anne-Lise Haenni, Roy A. Hall, Roger Hewson, Holly R. Hughes, Rakesh K. Jain, Miranda Gilda Jonson, Sandra Junglen, Boris Klempa, Jonas Klingström, Richard Kormelink, Amy J. Lambert, Stanley A. Langevin, Igor S. Lukashevich, Marco Marklewitz, Giovanni P. Martelli, Nicole Mielke-Ehret, Ali Mirazimi, Hans-Peter Mühlbach, Rayapati Naidu, Márcio Roberto Teixeira Nunes, Gustavo Palacios, Anna Papa, Janusz T. Pawęska, Clarence J. Peters, Alexander Plyusnin, Sheli R. Radoshitzky, Renato O. Resende, Victor Romanowski, Amadou Alpha Sall, Maria S. Salvato, Takahide Sasaya, Connie Schmaljohn, Xiǎohóng Shí, Yukio Shirako, Peter Simmonds, Manuela Sironi, Jin-Won Song, Jessica R. Spengler, Mark D. Stenglein, Robert B. Tesh, Massimo Turina, Tàiyún Wèi, Anna E. Whitfield, Shyi-Dong Yeh, F. Murilo Zerbini, Yong-Zhen Zhang, Xueping Zhou, Jens H. Kuhn. Taxonomy of the order Bunyavirales: second update 2018. Archives of Virology. 2019; 164 (3):927-941.
Chicago/Turabian StylePiet Maes; Scott Adkins; Sergey V. Alkhovsky; Tatjana Avšič Županc; Matthew J. Ballinger; Dennis A. Bente; Martin Beer; Éric Bergeron; Carol D. Blair; Thomas Briese; Michael J. Buchmeier; Felicity J. Burt; Charles H. Calisher; Rémi N. Charrel; Il Ryong Choi; J. Christopher S. Clegg; Juan Carlos De La Torre; Xavier De Lamballerie; Joseph L. DeRisi; Michele Digiaro; Mike Drebot; Hideki Ebihara; Toufic Elbeaino; Koray Ergünay; Charles F. Fulhorst; Aura R. Garrison; George Fú Gāo; Jean-Paul J. Gonzalez; Martin H. Groschup; Stephan Günther; Anne-Lise Haenni; Roy A. Hall; Roger Hewson; Holly R. Hughes; Rakesh K. Jain; Miranda Gilda Jonson; Sandra Junglen; Boris Klempa; Jonas Klingström; Richard Kormelink; Amy J. Lambert; Stanley A. Langevin; Igor S. Lukashevich; Marco Marklewitz; Giovanni P. Martelli; Nicole Mielke-Ehret; Ali Mirazimi; Hans-Peter Mühlbach; Rayapati Naidu; Márcio Roberto Teixeira Nunes; Gustavo Palacios; Anna Papa; Janusz T. Pawęska; Clarence J. Peters; Alexander Plyusnin; Sheli R. Radoshitzky; Renato O. Resende; Victor Romanowski; Amadou Alpha Sall; Maria S. Salvato; Takahide Sasaya; Connie Schmaljohn; Xiǎohóng Shí; Yukio Shirako; Peter Simmonds; Manuela Sironi; Jin-Won Song; Jessica R. Spengler; Mark D. Stenglein; Robert B. Tesh; Massimo Turina; Tàiyún Wèi; Anna E. Whitfield; Shyi-Dong Yeh; F. Murilo Zerbini; Yong-Zhen Zhang; Xueping Zhou; Jens H. Kuhn. 2019. "Taxonomy of the order Bunyavirales: second update 2018." Archives of Virology 164, no. 3: 927-941.
In 2012, the genome of a novel rhabdovirus, Bas-Congo virus, was discovered in the acute-phase serum of a Congolese patient with presumed viral hemorrhagic fever. In the absence of a replicating virus isolate, fulfilling Koch's postulates to determine whether Bas-Congo virus is indeed a human virus and/or pathogen has been impossible. However, experiments with vesiculoviral particles pseudotyped with Bas-Congo glycoprotein suggested that Bas-Congo virus particles can enter cells from multiple animals, including humans. In 2015, genomes of two related viruses, Ekpoma virus 1 and Ekpoma virus 2, were detected in human sera in Nigeria. Isolates could not be obtained. Phylogenetic analyses led to the classification of Bas-Congo virus, Ekpoma virus 1, and Ekpoma virus 2 in the same genus, Tibrovirus, together with five biting midge-borne rhabdoviruses (i.e., Beatrice Hill virus, Bivens Arm virus, Coastal Plains virus, Sweetwater Branch virus, and Tibrogargan virus) not known to infect humans. Using individual recombinant vesiculoviruses expressing the glycoproteins of all eight known tibroviruses and more than 75 cell lines representing different animal species, we demonstrate that the glycoproteins of all tibroviruses can mediate vesiculovirus particle entry into human, bat, nonhuman primate, cotton rat, boa constrictor, and Asian tiger mosquito cells. Using four of five isolated authentic tibroviruses (i.e., Bivens Arm virus, Coastal Plains virus, Sweetwater Branch virus, and Tibrogargan virus), our experiments indicate that many cell types may be partially resistant to tibrovirus replication after virion cell entry. Consequently, experimental data solely obtained from experiments using tibrovirus surrogate systems (e.g., vesiculoviral pseudotypes, recombinant vesiculoviruses) cannot be used to predict whether Bas-Congo virus, or any other tibrovirus, infects humans.
Yingyun Cai; Shuiqing Yu; Rohit Jangra; Elena Postnikova; Jiro Wada; Robert Tesh; Sean Whelan; Michael Lauck; Michael Wiley; Courtney Finch; Sheli Radoshitzky; David O'connor; Gustavo Palacios; Kartik Chandran; Charles Chiu; Jens Kuhn. Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry. 2018, 507350 .
AMA StyleYingyun Cai, Shuiqing Yu, Rohit Jangra, Elena Postnikova, Jiro Wada, Robert Tesh, Sean Whelan, Michael Lauck, Michael Wiley, Courtney Finch, Sheli Radoshitzky, David O'connor, Gustavo Palacios, Kartik Chandran, Charles Chiu, Jens Kuhn. Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry. . 2018; ():507350.
Chicago/Turabian StyleYingyun Cai; Shuiqing Yu; Rohit Jangra; Elena Postnikova; Jiro Wada; Robert Tesh; Sean Whelan; Michael Lauck; Michael Wiley; Courtney Finch; Sheli Radoshitzky; David O'connor; Gustavo Palacios; Kartik Chandran; Charles Chiu; Jens Kuhn. 2018. "Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry." , no. : 507350.
Lassa virus (LASV), a mammarenavirus, infects an estimated 100,000–300,000 individuals yearly in western Africa and frequently causes lethal disease. Currently, no LASV-specific antivirals or vaccines are commercially available for prevention or treatment of Lassa fever, the disease caused by LASV. The development of medical countermeasure screening platforms is a crucial step to yield licensable products. Using reverse genetics, we generated a recombinant wild-type LASV (rLASV-WT) and a modified version thereof encoding a cleavable green fluorescent protein (GFP) as a reporter for rapid and quantitative detection of infection (rLASV-GFP). Both rLASV-WT and wild-type LASV exhibited similar growth kinetics in cultured cells, whereas growth of rLASV-GFP was slightly impaired. GFP reporter expression by rLASV-GFP remained stable over several serial passages in Vero cells. Using two well-characterized broad-spectrum antivirals known to inhibit LASV infection, favipiravir and ribavirin, we demonstrate that rLASV-GFP is a suitable screening tool for the identification of LASV infection inhibitors. Building on these findings, we established a rLASV-GFP-based high-throughput drug discovery screen and an rLASV-GFP-based antibody neutralization assay. Both platforms, now available as a standard tool at the IRF-Frederick (an international resource), will accelerate anti-LASV medical countermeasure discovery and reduce costs of antiviral screens in maximum containment laboratories.
Yíngyún Caì; Masaharu Iwasaki; Brett F. Beitzel; Shuīqìng Yú; Elena N. Postnikova; Beatrice Cubitt; Lisa Evans Dewald; Sheli R. Radoshitzky; Laura Bollinger; Peter B. Jahrling; Gustavo F. Palacios; Juan C. De La Torre; Jens H. Kuhn. Recombinant Lassa Virus Expressing Green Fluorescent Protein as a Tool for High-Throughput Drug Screens and Neutralizing Antibody Assays. Viruses 2018, 10, 655 .
AMA StyleYíngyún Caì, Masaharu Iwasaki, Brett F. Beitzel, Shuīqìng Yú, Elena N. Postnikova, Beatrice Cubitt, Lisa Evans Dewald, Sheli R. Radoshitzky, Laura Bollinger, Peter B. Jahrling, Gustavo F. Palacios, Juan C. De La Torre, Jens H. Kuhn. Recombinant Lassa Virus Expressing Green Fluorescent Protein as a Tool for High-Throughput Drug Screens and Neutralizing Antibody Assays. Viruses. 2018; 10 (11):655.
Chicago/Turabian StyleYíngyún Caì; Masaharu Iwasaki; Brett F. Beitzel; Shuīqìng Yú; Elena N. Postnikova; Beatrice Cubitt; Lisa Evans Dewald; Sheli R. Radoshitzky; Laura Bollinger; Peter B. Jahrling; Gustavo F. Palacios; Juan C. De La Torre; Jens H. Kuhn. 2018. "Recombinant Lassa Virus Expressing Green Fluorescent Protein as a Tool for High-Throughput Drug Screens and Neutralizing Antibody Assays." Viruses 10, no. 11: 655.
Summary Sexual transmission of filoviruses was first reported in 1968 after an outbreak of Marburg virus (MARV) disease and recently caused flare-ups of Ebola virus disease in the 2013–2016 outbreak. How filoviruses establish testicular persistence and are shed in semen remain unknown. We discovered that persistent MARV infection of seminiferous tubules, an immune-privileged site that harbors sperm production, is a relatively common event in crab-eating macaques that survived infection after antiviral treatment. Persistence triggers severe testicular damage, including spermatogenic cell depletion and inflammatory cell invasion. MARV mainly persists in Sertoli cells, leading to breakdown of the blood-testis barrier formed by inter-Sertoli cell tight junctions. This disruption is accompanied by local infiltration of immunosuppressive CD4+Foxp3+ regulatory T cells. Our study elucidates cellular events associated with testicular persistence that may promote sexual transmission of filoviruses and suggests that targeting immunosuppression may be warranted to clear filovirus persistence in damaged immune-privileged sites.
Kayla M. Coffin; Jun Liu; Travis K. Warren; Candace D. Blancett; Kathleen A. Kuehl; Donald K. Nichols; Jeremy J. Bearss; Christopher W. Schellhase; Cary J. Retterer; Jessica M. Weidner; Sheli Radoshitzky; Jennifer M. Brannan; Anthony P. Cardile; John M. Dye; Gustavo Palacios; Mei G. Sun; Jens H. Kuhn; Sina Bavari; Xiankun Zeng. Persistent Marburg Virus Infection in the Testes of Nonhuman Primate Survivors. Cell Host & Microbe 2018, 24, 405 -416.e3.
AMA StyleKayla M. Coffin, Jun Liu, Travis K. Warren, Candace D. Blancett, Kathleen A. Kuehl, Donald K. Nichols, Jeremy J. Bearss, Christopher W. Schellhase, Cary J. Retterer, Jessica M. Weidner, Sheli Radoshitzky, Jennifer M. Brannan, Anthony P. Cardile, John M. Dye, Gustavo Palacios, Mei G. Sun, Jens H. Kuhn, Sina Bavari, Xiankun Zeng. Persistent Marburg Virus Infection in the Testes of Nonhuman Primate Survivors. Cell Host & Microbe. 2018; 24 (3):405-416.e3.
Chicago/Turabian StyleKayla M. Coffin; Jun Liu; Travis K. Warren; Candace D. Blancett; Kathleen A. Kuehl; Donald K. Nichols; Jeremy J. Bearss; Christopher W. Schellhase; Cary J. Retterer; Jessica M. Weidner; Sheli Radoshitzky; Jennifer M. Brannan; Anthony P. Cardile; John M. Dye; Gustavo Palacios; Mei G. Sun; Jens H. Kuhn; Sina Bavari; Xiankun Zeng. 2018. "Persistent Marburg Virus Infection in the Testes of Nonhuman Primate Survivors." Cell Host & Microbe 24, no. 3: 405-416.e3.
Several arenaviruses cause hemorrhagic fever (HF) diseases that are associated with high morbidity and mortality in humans. Accordingly, HF arenaviruses have been listed as top-priority emerging diseases for which countermeasures are urgently needed. Because arenavirus nucleoprotein (NP) plays critical roles in both virus multiplication and immune-evasion, we used an unbiased proteomic approach to identify NP-interacting proteins in human cells. DDX3, a DEAD-box ATP-dependent-RNA-helicase, interacted with NP in both NP-transfected and virus-infected cells. Importantly, DDX3 deficiency compromised the propagation of both Old and New World arenaviruses, including the HF arenaviruses Lassa and Junin viruses. The DDX3 role in promoting arenavirus multiplication associated with both a previously un-recognized DDX3 inhibitory role in type I interferon production in arenavirus infected cells and a positive DDX3 effect on arenavirus RNA synthesis that was dependent on its ATPase and Helicase activities. Our results uncover novel mechanisms used by arenaviruses to exploit the host machinery and subvert immunity, singling out DDX3 as a potential host target for developing new therapies against highly pathogenic arenaviruses. Arenaviruses include severe pathogens causing hemorrhagic fevers and have been recently incorporated by the World Health Organization in a list of critical emerging diseases for which additional research and identification of clinical targets is urgently required. A better understanding of how viral proteins interact with host cellular factors to favor arenavirus multiplication can illuminate novel pipelines on therapeutic strategies. Here we demonstrated that the ATP-dependent RNA helicase DDX3 interacted with the arenavirus nucleoprotein, which displays fundamental functions in different steps of the viral-cycle. Our work also revealed an unexpected new biology on the role that DDX3 might play during viral infections. In sharp contrast to previous studies showing DDX3 enhancement of IFN-I induction, we demonstrated that DDX3 suppressed IFN-I production, contributing to a DDX3 pro-viral effect late after arenavirus infection. We also showed that early after infection, DDX3 pro-viral role was IFN-I independent and was mediated by facilitation of viral RNA synthesis via DDX3 ATPase and Helicase activities. Altogether, our study established DDX3 as a critical host interacting partner of the arenavirus nucleoprotein and demonstrated two previously unrecognized DDX3-dependent strategies by which these deadly viruses exploit the host cellular machinery and suppress immunity.
María Eugenia Loureiro; Andre Luiz Zorzetto-Fernandes; Sheli Radoshitzky; Xiaoli Chi; Simone Dallari; Nuha Marooki; Psylvia Lèger; Sabrina Foscaldi; Vince Harjono; Sonia Sharma; Brian M. Zid; Nora López; Juan Carlos De La Torre; Sina Bavari; Elina Zúñiga. DDX3 suppresses type I interferons and favors viral replication during Arenavirus infection. PLOS Pathogens 2018, 14, e1007125 .
AMA StyleMaría Eugenia Loureiro, Andre Luiz Zorzetto-Fernandes, Sheli Radoshitzky, Xiaoli Chi, Simone Dallari, Nuha Marooki, Psylvia Lèger, Sabrina Foscaldi, Vince Harjono, Sonia Sharma, Brian M. Zid, Nora López, Juan Carlos De La Torre, Sina Bavari, Elina Zúñiga. DDX3 suppresses type I interferons and favors viral replication during Arenavirus infection. PLOS Pathogens. 2018; 14 (7):e1007125.
Chicago/Turabian StyleMaría Eugenia Loureiro; Andre Luiz Zorzetto-Fernandes; Sheli Radoshitzky; Xiaoli Chi; Simone Dallari; Nuha Marooki; Psylvia Lèger; Sabrina Foscaldi; Vince Harjono; Sonia Sharma; Brian M. Zid; Nora López; Juan Carlos De La Torre; Sina Bavari; Elina Zúñiga. 2018. "DDX3 suppresses type I interferons and favors viral replication during Arenavirus infection." PLOS Pathogens 14, no. 7: e1007125.
Ebola virus (EBOV) disease is a viral hemorrhagic fever with a high case-fatality rate in humans. This disease is caused by four members of the filoviral genus Ebolavirus, including EBOV. The natural hosts reservoirs of ebolaviruses remain to be identified. Glycoprotein 2 of reptarenaviruses, known to infect only boa constrictors and pythons, is similar in sequence and structure to ebolaviral glycoprotein 2, suggesting that EBOV may be able to infect reptilian cells. Therefore, we serially passaged EBOV and a distantly related filovirus, Marburg virus (MARV), in boa constrictor JK cells and characterized viral infection/replication and mutational frequency by confocal imaging and sequencing. We observed that EBOV efficiently infected and replicated in JK cells, but MARV did not. In contrast to most cell lines, EBOV-infected JK cells did not result in an obvious cytopathic effect. Surprisingly, genomic characterization of serial-passaged EBOV in JK cells revealed that genomic adaptation was not required for infection. Deep sequencing coverage (>10,000×) demonstrated the existence of only a single nonsynonymous variant (EBOV glycoprotein precursor pre-GP T544I) of unknown significance within the viral population that exhibited a shift in frequency of at least 10 per cent over six serial passages. In summary, we present the first reptilian cell line that replicates a filovirus at high titers, and for the first time demonstrate a filovirus genus-specific restriction to MARV in a cell line. Our data suggest the possibility that there may be differences between the natural host spectra of ebolaviruses and marburgviruses.
Greg Fedewa; Sheli R Radoshitzky; Xiǎolì Chī; Lián Dǒng; Xiankun Zeng; Melissa Spear; Nicolas Strauli; Melinda Ng; Kartik Chandran; Mark D Stenglein; Ryan D Hernandez; Peter B Jahrling; Jens H Kuhn; Joseph L DeRisi. Ebola virus, but not Marburg virus, replicates efficiently and without required adaptation in snake cells. Virus Evolution 2018, 4, vey034 .
AMA StyleGreg Fedewa, Sheli R Radoshitzky, Xiǎolì Chī, Lián Dǒng, Xiankun Zeng, Melissa Spear, Nicolas Strauli, Melinda Ng, Kartik Chandran, Mark D Stenglein, Ryan D Hernandez, Peter B Jahrling, Jens H Kuhn, Joseph L DeRisi. Ebola virus, but not Marburg virus, replicates efficiently and without required adaptation in snake cells. Virus Evolution. 2018; 4 (2):vey034.
Chicago/Turabian StyleGreg Fedewa; Sheli R Radoshitzky; Xiǎolì Chī; Lián Dǒng; Xiankun Zeng; Melissa Spear; Nicolas Strauli; Melinda Ng; Kartik Chandran; Mark D Stenglein; Ryan D Hernandez; Peter B Jahrling; Jens H Kuhn; Joseph L DeRisi. 2018. "Ebola virus, but not Marburg virus, replicates efficiently and without required adaptation in snake cells." Virus Evolution 4, no. 2: vey034.
In 2018, the family Arenaviridae was expanded by inclusion of 1 new genus and 5 novel species. At the same time, the recently established order Bunyavirales was expanded by 3 species. This article presents the updated taxonomy of the family Arenaviridae and the order Bunyavirales as now accepted by the International Committee on Taxonomy of Viruses (ICTV) and summarizes additional taxonomic proposals that may affect the order in the near future.
Piet Maes; Sergey V. Alkhovsky; Yīmíng Bào; Martin Beer; Monica Birkhead; Thomas Briese; Michael J. Buchmeier; Charles H. Calisher; Rémi N. Charrel; Il Ryong Choi; Christopher S. Clegg; Juan Carlos De La Torre; Eric Delwart; Joseph L. DeRisi; Patrick L. Di Bello; Francesco Di Serio; Michele Digiaro; Valerian V. Dolja; Christian Drosten; Tobiasz Z. Druciarek; Jiang Du; Hideki Ebihara; Toufic Elbeaino; Rose C. Gergerich; Amethyst N. Gillis; Jean-Paul J. Gonzalez; Anne-Lise Haenni; Jussi Hepojoki; Udo Hetzel; Thiện Hồ; Ní Hóng; Rakesh K. Jain; Petrus Jansen Van Vuren; Qi Jin; Miranda Gilda Jonson; Sandra Junglen; Karen E. Keller; Alan Kemp; Anja Kipar; Nikola O. Kondov; Eugene V. Koonin; Richard Kormelink; Yegor Korzyukov; Mart Krupovic; Amy J. Lambert; Alma G. Laney; Matthew LeBreton; Igor S. Lukashevich; Marco Marklewitz; Wanda Markotter; Giovanni P. Martelli; Robert R. Martin; Nicole Mielke-Ehret; Hans-Peter Mühlbach; Beatriz Navarro; Terry Fei Fan Ng; Márcio Roberto Teixeira Nunes; Gustavo Palacios; Janusz T. Pawęska; Clarence J. Peters; Alexander Plyusnin; Sheli R. Radoshitzky; Victor Romanowski; Pertteli Salmenperä; Maria S. Salvato; Hélène Sanfaçon; Takahide Sasaya; Connie Schmaljohn; Bradley S. Schneider; Yukio Shirako; Stuart Siddell; Tarja A. Sironen; Mark D. Stenglein; Nadia Storm; Harikishan Sudini; Robert B. Tesh; Ioannis E. Tzanetakis; Mangala Uppala; Olli Vapalahti; Nikos Vasilakis; Peter J. Walker; Guópíng Wáng; Lìpíng Wáng; Yànxiăng Wáng; Tàiyún Wèi; Michael R. Wiley; Yuri I. Wolf; Nathan D. Wolfe; Zhìqiáng Wú; Wénxìng Xú; Li Yang; Zuòkūn Yāng; Shyi-Dong Yeh; Yǒng-Zhèn Zhāng; Yàzhōu Zhèng; Xueping Zhou; Chénxī Zhū; Florian Zirkel; Jens H. Kuhn. Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018. Archives of Virology 2018, 163, 2295 -2310.
AMA StylePiet Maes, Sergey V. Alkhovsky, Yīmíng Bào, Martin Beer, Monica Birkhead, Thomas Briese, Michael J. Buchmeier, Charles H. Calisher, Rémi N. Charrel, Il Ryong Choi, Christopher S. Clegg, Juan Carlos De La Torre, Eric Delwart, Joseph L. DeRisi, Patrick L. Di Bello, Francesco Di Serio, Michele Digiaro, Valerian V. Dolja, Christian Drosten, Tobiasz Z. Druciarek, Jiang Du, Hideki Ebihara, Toufic Elbeaino, Rose C. Gergerich, Amethyst N. Gillis, Jean-Paul J. Gonzalez, Anne-Lise Haenni, Jussi Hepojoki, Udo Hetzel, Thiện Hồ, Ní Hóng, Rakesh K. Jain, Petrus Jansen Van Vuren, Qi Jin, Miranda Gilda Jonson, Sandra Junglen, Karen E. Keller, Alan Kemp, Anja Kipar, Nikola O. Kondov, Eugene V. Koonin, Richard Kormelink, Yegor Korzyukov, Mart Krupovic, Amy J. Lambert, Alma G. Laney, Matthew LeBreton, Igor S. Lukashevich, Marco Marklewitz, Wanda Markotter, Giovanni P. Martelli, Robert R. Martin, Nicole Mielke-Ehret, Hans-Peter Mühlbach, Beatriz Navarro, Terry Fei Fan Ng, Márcio Roberto Teixeira Nunes, Gustavo Palacios, Janusz T. Pawęska, Clarence J. Peters, Alexander Plyusnin, Sheli R. Radoshitzky, Victor Romanowski, Pertteli Salmenperä, Maria S. Salvato, Hélène Sanfaçon, Takahide Sasaya, Connie Schmaljohn, Bradley S. Schneider, Yukio Shirako, Stuart Siddell, Tarja A. Sironen, Mark D. Stenglein, Nadia Storm, Harikishan Sudini, Robert B. Tesh, Ioannis E. Tzanetakis, Mangala Uppala, Olli Vapalahti, Nikos Vasilakis, Peter J. Walker, Guópíng Wáng, Lìpíng Wáng, Yànxiăng Wáng, Tàiyún Wèi, Michael R. Wiley, Yuri I. Wolf, Nathan D. Wolfe, Zhìqiáng Wú, Wénxìng Xú, Li Yang, Zuòkūn Yāng, Shyi-Dong Yeh, Yǒng-Zhèn Zhāng, Yàzhōu Zhèng, Xueping Zhou, Chénxī Zhū, Florian Zirkel, Jens H. Kuhn. Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018. Archives of Virology. 2018; 163 (8):2295-2310.
Chicago/Turabian StylePiet Maes; Sergey V. Alkhovsky; Yīmíng Bào; Martin Beer; Monica Birkhead; Thomas Briese; Michael J. Buchmeier; Charles H. Calisher; Rémi N. Charrel; Il Ryong Choi; Christopher S. Clegg; Juan Carlos De La Torre; Eric Delwart; Joseph L. DeRisi; Patrick L. Di Bello; Francesco Di Serio; Michele Digiaro; Valerian V. Dolja; Christian Drosten; Tobiasz Z. Druciarek; Jiang Du; Hideki Ebihara; Toufic Elbeaino; Rose C. Gergerich; Amethyst N. Gillis; Jean-Paul J. Gonzalez; Anne-Lise Haenni; Jussi Hepojoki; Udo Hetzel; Thiện Hồ; Ní Hóng; Rakesh K. Jain; Petrus Jansen Van Vuren; Qi Jin; Miranda Gilda Jonson; Sandra Junglen; Karen E. Keller; Alan Kemp; Anja Kipar; Nikola O. Kondov; Eugene V. Koonin; Richard Kormelink; Yegor Korzyukov; Mart Krupovic; Amy J. Lambert; Alma G. Laney; Matthew LeBreton; Igor S. Lukashevich; Marco Marklewitz; Wanda Markotter; Giovanni P. Martelli; Robert R. Martin; Nicole Mielke-Ehret; Hans-Peter Mühlbach; Beatriz Navarro; Terry Fei Fan Ng; Márcio Roberto Teixeira Nunes; Gustavo Palacios; Janusz T. Pawęska; Clarence J. Peters; Alexander Plyusnin; Sheli R. Radoshitzky; Victor Romanowski; Pertteli Salmenperä; Maria S. Salvato; Hélène Sanfaçon; Takahide Sasaya; Connie Schmaljohn; Bradley S. Schneider; Yukio Shirako; Stuart Siddell; Tarja A. Sironen; Mark D. Stenglein; Nadia Storm; Harikishan Sudini; Robert B. Tesh; Ioannis E. Tzanetakis; Mangala Uppala; Olli Vapalahti; Nikos Vasilakis; Peter J. Walker; Guópíng Wáng; Lìpíng Wáng; Yànxiăng Wáng; Tàiyún Wèi; Michael R. Wiley; Yuri I. Wolf; Nathan D. Wolfe; Zhìqiáng Wú; Wénxìng Xú; Li Yang; Zuòkūn Yāng; Shyi-Dong Yeh; Yǒng-Zhèn Zhāng; Yàzhōu Zhèng; Xueping Zhou; Chénxī Zhū; Florian Zirkel; Jens H. Kuhn. 2018. "Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018." Archives of Virology 163, no. 8: 2295-2310.
The majority of viruses causing hemorrhagic fever in humans are Risk Group 3 or 4 pathogens and, therefore, can only be handled in biosafety level 3 or 4 (BSL-3/4) containment laboratories. The restricted number of such laboratories, the substantial financial requirements to maintain them, and safety concerns for the laboratory workers pose formidable challenges for rapid medical countermeasure discovery and evaluation. BSL-2 surrogate systems are a less challenging, cheap, and fast alternative to the use of live high-consequence viruses for dissecting and targeting individual steps of viral lifecycles with a diminished threat to the laboratory worker. Typical surrogate systems are virion-like particles (VLPs), transcriptionally active (“infectious”) VLPs, minigenome systems, recombinant heterotypic viruses encoding proteins of target viruses, and vesiculoviral or retroviral pseudotype systems. Here, we outline the use of retroviral pseudotypes for identification of antivirals against BSL-4 pathogens.
Sheli R. Radoshitzky; Veronica Soloveva; Dima Gharaibeh; Jens H. Kuhn; Sina Bavari. Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses. Advanced Structural Safety Studies 2017, 1604, 393 -403.
AMA StyleSheli R. Radoshitzky, Veronica Soloveva, Dima Gharaibeh, Jens H. Kuhn, Sina Bavari. Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses. Advanced Structural Safety Studies. 2017; 1604 ():393-403.
Chicago/Turabian StyleSheli R. Radoshitzky; Veronica Soloveva; Dima Gharaibeh; Jens H. Kuhn; Sina Bavari. 2017. "Retrovirus-Based Surrogate Systems for BSL-2 High-Throughput Screening of Antivirals Targeting BSL-3/4 Hemorrhagic Fever-Causing Viruses." Advanced Structural Safety Studies 1604, no. : 393-403.
Ebola virus (EBOV) persistence in asymptomatic humans and Ebola virus disease (EVD) sequelae have emerged as significant public health concerns since the 2013–2016 EVD outbreak in Western Africa. Until now, studying how EBOV disseminates into and persists in immune-privileged sites was impossible due to the absence of a suitable animal model. Here, we detect persistent EBOV replication coinciding with systematic inflammatory responses in otherwise asymptomatic rhesus monkeys that had survived infection in the absence of or after treatment with candidate medical countermeasures. We document progressive EBOV dissemination into the eyes, brain and testes through vascular structures, similar to observations in humans. We identify CD68+ cells (macrophages/monocytes) as the cryptic EBOV reservoir cells in the vitreous humour and its immediately adjacent tissue, in the tubular lumina of the epididymides, and in foci of histiocytic inflammation in the brain, but not in organs typically affected during acute infection. In conclusion, our data suggest that persistent EBOV infection in rhesus monkeys could serve as a model for persistent EBOV infection in humans, and we demonstrate that promising candidate medical countermeasures may not completely clear EBOV infection. A rhesus monkey model may lay the foundation to study EVD sequelae and to develop therapies to abolish EBOV persistence.
Xiankun Zeng; Candace D. Blancett; Keith A. Koistinen; Christopher W. Schellhase; Jeremy J. Bearss; Sheli Radoshitzky; Shelley P. Honnold; Taylor B. Chance; Travis K. Warren; Jeffrey W. Froude; Kathleen A. Cashman; John M. Dye; Sina Bavari; Gustavo Palacios; Jens H. Kuhn; Mei G. Sun. Identification and pathological characterization of persistent asymptomatic Ebola virus infection in rhesus monkeys. Nature Microbiology 2017, 2, 17113 .
AMA StyleXiankun Zeng, Candace D. Blancett, Keith A. Koistinen, Christopher W. Schellhase, Jeremy J. Bearss, Sheli Radoshitzky, Shelley P. Honnold, Taylor B. Chance, Travis K. Warren, Jeffrey W. Froude, Kathleen A. Cashman, John M. Dye, Sina Bavari, Gustavo Palacios, Jens H. Kuhn, Mei G. Sun. Identification and pathological characterization of persistent asymptomatic Ebola virus infection in rhesus monkeys. Nature Microbiology. 2017; 2 (9):17113.
Chicago/Turabian StyleXiankun Zeng; Candace D. Blancett; Keith A. Koistinen; Christopher W. Schellhase; Jeremy J. Bearss; Sheli Radoshitzky; Shelley P. Honnold; Taylor B. Chance; Travis K. Warren; Jeffrey W. Froude; Kathleen A. Cashman; John M. Dye; Sina Bavari; Gustavo Palacios; Jens H. Kuhn; Mei G. Sun. 2017. "Identification and pathological characterization of persistent asymptomatic Ebola virus infection in rhesus monkeys." Nature Microbiology 2, no. 9: 17113.
Medical countermeasures (MCMs) against virus infections ideally prevent the adsorption or entry of virions into target cells, thereby circumventing infection. Recent significant advances in elucidating the mechanism of Ebola virus (EBOV) host-cell penetration include the involvement of two-pore channels at the early stage of entry, and identification of cellular proteases for EBOV spike glycoprotein maturation and the intracellular EBOV receptor, Niemann–Pick type C1. This improved understanding of the initial steps of EBOV infection is now increasingly applied to rapid development of candidate MCMs, some of which have already entered the clinic. Candidate MCMs discussed include antibodies, small molecules and peptides that target various stages of the described EBOV cell-entry pathway. In this review, we summarize the currently known spectrum of EBOV cell-entry inhibitors, describe their mechanism of action and evaluate their potential for future development.
Janie Liang; Rohit K Jangra; Laura Bollinger; Jiro Wada; Sheli Radoshitzky; Kartik Chandran; Peter B Jahrling; Jens H Kuhn; Kenneth S Jensen. Candidate medical countermeasures targeting Ebola virus cell entry. Future Virology 2017, 12, 119 -140.
AMA StyleJanie Liang, Rohit K Jangra, Laura Bollinger, Jiro Wada, Sheli Radoshitzky, Kartik Chandran, Peter B Jahrling, Jens H Kuhn, Kenneth S Jensen. Candidate medical countermeasures targeting Ebola virus cell entry. Future Virology. 2017; 12 (3):119-140.
Chicago/Turabian StyleJanie Liang; Rohit K Jangra; Laura Bollinger; Jiro Wada; Sheli Radoshitzky; Kartik Chandran; Peter B Jahrling; Jens H Kuhn; Kenneth S Jensen. 2017. "Candidate medical countermeasures targeting Ebola virus cell entry." Future Virology 12, no. 3: 119-140.