This study investigates the role of soil moisture on the threshold runoff response in a small headwater catchment in the Italian Alps that is characterised by steep hillslopes and a distinct riparian zone. This study focuses on: (i) the threshold soil moisture-runoff relationship and the influence of catchment topography on this relation; (ii) the temporal dynamics of soil moisture, streamflow and groundwater that characterize the catchment's response to rainfall during dry and wet periods; and (iii) the combined effect of antecedent wetness conditions and rainfall amount on hillslope and riparian runoff. Our results highlight the strong control exerted by soil moisture on runoff in this catchment: a sharp threshold exists in the relationship between soil water content and runoff coefficient, streamflow, and hillslope-averaged depth to water table. Low runoff ratios were likely related to the response of the riparian zone, which was almost always close to saturation. High runoff ratios occurred during wet antecedent conditions, when the soil moisture threshold was exceeded. In these cases, subsurface flow was activated on hillslopes, which became a major contributor to runoff. Antecedent wetness conditions also controlled the catchment's response time: during dry periods, streamflow reacted and peaked prior to hillslope soil moisture whereas during wet conditions the opposite occurred. This difference resulted in a hysteretic behaviour in the soil moisture-streamflow relationship. Finally, the influence of antecedent moisture conditions on runoff was also evident in the relation between cumulative rainfall and total stormflow. Small storms during dry conditions produced low stormflow amounts, likely mainly from overland flow from the near saturated riparian zone. Conversely, for rainfall events during wet conditions, higher stormflow values were observed and hillslopes must have contributed to streamflow.

The time that water takes to travel through the terrestrial hydrological cycle and the critical zone is of great interest in Earth system sciences with broad implications for water quality and quantity. Most water age studies to date have focused on individual compartments (or subdisciplines) of the hydrological cycle such as the unsaturated or saturated zone, vegetation, atmosphere, or rivers. However, recent studies have shown that processes at the interfaces between the hydrological compartments (e.g., soil‐atmosphere or soil‐groundwater) govern the age distribution of the water fluxes between these compartments and thus can greatly affect water travel times. The broad variation from complete to nearly absent mixing of water at these interfaces affects the water ages in the compartments. This is especially the case for the highly heterogeneous critical zone between the top of the vegetation and the bottom of the groundwater storage. Here, we review a wide variety of studies about water ages in the critical zone and provide (1) an overview of new prospects and challenges in the use of hydrological tracers to study water ages, (2) a discussion of the limiting assumptions linked to our lack of process understanding and methodological transfer of water age estimations to individual disciplines or compartments, and (3) a vision for how to improve future interdisciplinary efforts to better understand the feedbacks between the atmosphere, vegetation, soil, groundwater, and surface water that control water ages in the critical zone.

Stable water isotopes are widely used in ecohydrology to trace the transport, storage, and mixing of water on its journey through landscapes and ecosystems. Evaporation leaves a characteristic signature on the isotopic composition of the water that is left behind, such that in dual-isotope space, evaporated waters plot below the local meteoric water line (LMWL) that characterizes precipitation. Soil and xylem water samples can often plot below the LMWL as well, suggesting that they have also been influenced by evaporation. These soil and xylem water samples frequently plot along linear trends in dual-isotope space. These trend lines are often termed “evaporation lines” and their intersection with the LMWL is often interpreted as the isotopic composition of the precipitation source water. Here we use numerical experiments based on established isotope fractionation theory to show that these trend lines are often by-products of the seasonality in evaporative fractionation and in the isotopic composition of precipitation. Thus, they are often not true evaporation lines, and, if interpreted as such, can yield highly biased estimates of the isotopic composition of the source water.

In this commentary, we summarize and build upon discussions that emerged during the workshop “Isotope-based studies of water partitioning and plant–soil interactions in forested and agricultural environments” held in San Casciano in Val di Pesa, Italy, in September 2017. Quantifying and understanding how water cycles through the Earth's critical zone is important to provide society and policymakers with the scientific background to manage water resources sustainably, especially considering the ever-increasing worldwide concern about water scarcity. Stable isotopes of hydrogen and oxygen in water have proven to be a powerful tool for tracking water fluxes in the critical zone. However, both mechanistic complexities (e.g. mixing and fractionation processes, heterogeneity of natural systems) and methodological issues (e.g. lack of standard protocols to sample specific compartments, such as soil water and xylem water) limit the application of stable water isotopes in critical-zone science. In this commentary, we examine some of the opportunities and critical challenges of isotope-based ecohydrological applications and outline new perspectives focused on interdisciplinary research opportunities for this important tool in water and environmental science.