Vernonia glabra is used by Malawian traditional healers to treat a myriad of ailments including shingles, diabetes, dysentery, diarrhoea, cough, wounds, gonorrhoea, and gastrointestinal problems. This study evaluated phytoconstituents and the in vitro antimycobacterial activities of the crude extracts and fractions from roots of Vernonia glabra. Agar disk diffusion and modified broth microdilution assays were used to determine the antimycobacterial activities of dichloromethane (DCM) and ethyl acetate (EtOAc) crude extracts and fractions from the roots. Gas chromatography-mass spectrometry (GC-MS) and Fourier-transform infrared spectroscopy (FTIR) analyses were used to characterise the major compounds in the crude extracts and fractions. The EtOAc fraction exhibited the highest antimycobacterial activity against Mycobacterium tuberculosis H37Rv. The EtOAc fraction achieved a minimum inhibitory concentration (MIC) of 4.88μg/mL and a half-maximal inhibitory concentration (IC₅₀) value of 38.37μg/mL, whereas gentamycin, a standard drug, achieved MIC and IC₅₀ values of 3.13μg/mL and 3.88μg/mL, respectively. GC-MS and FTIR analyses of the EtOAc fractions revealed the presence of hexadecenoic acid, methyl ester (0.39), diisooctyl phthalate (24.56), oxalic acid, allyl undecyl ester (4.66), bis(2-ethylhexylphthalate) (5.41), octacosane (51.12), tetradecane (12.63), phenol, 3,5-bis(1,1-dimethylethyl) (0.07), and 2-hydroxy-4-methylbenzaldehyde (6.47). The identified compounds are reported in the literature to possess antimicrobial and anticancer activities. It was evident from the comparison of the hill slope (HS) values of the dose-response curves of the fraction (1.005) and its crude extract (1.322) that repeated fractionation improved the activity. Therefore, this study showed that the roots of Vernonia glabra present a potential source of new leads for antimycobacterial drugs.

This study presents an assessment of ambient air quality in Chichiri and Malawi University of Business and Applied Sciences (MUBAS) locations, Blantyre City, Southern Malawi. The study aimed at assessing temporal trends, identifying exceedance of thresholds, investigating relationships between pollutants and meteorological factors, and exploring the predictability of air quality index (AQI). Five pollutants: ${\mathrm{P}\mathrm{M}}_{2.5}$, ${\mathrm{P}\mathrm{M}}_{10}$, ${\mathrm{N}\mathrm{O}}_{\mathrm{x}}$, ${\mathrm{C}\mathrm{O}}_2$ and TVOC were assessed over a two-month period using fixed low-cost sensors. Daily and hourly temporal analysis showed that pollutants peak during morning and evening hours. A significant number of exceedances for ${\mathrm{P}\mathrm{M}}_{2.5}$ and ${\mathrm{P}\mathrm{M}}_{10}$ were observed when compared to indicative thresholds. Chichiri exhibited more frequent AQI classifications in the “unhealthy” range. A strong positive relationship between ${\mathrm{P}\mathrm{M}}_{2.5}$ and ${\mathrm{P}\mathrm{M}}_{10}$ (r $=$ 0.84) and positive correlations between ${\mathrm{N}\mathrm{O}}_{\mathrm{x}}$ and ${\mathrm{C}\mathrm{O}}_2$ were observed. A multiple linear regression model achieved a high coefficient of determination (${\mathrm{R}}^2$ $=$ 0.938), identifying ${\mathrm{P}\mathrm{M}}_{10}$ and ${\mathrm{N}\mathrm{O}}_{\mathrm{x}}$ as dominant predictors of AQI variability. Temperature and humidity showed modest inverse relationship with AQI, suggesting dispersion effects. A comparison with African cities showed that the study areas’ pollution levels were within regional norms, but that there is a need for targeted mitigation. These findings underscore the importance of continuous monitoring, data-driven policy making and regional collaboration to address urban air quality challenges.