Brassica carinata, a nonfood oilseed crop, is used to produce renewable fuels because of its high oil content and favorable fatty acid profile. Production in the southeastern United States is relatively new, and information on agronomic management practices to optimize growth and yield is limited. Since optimal seeding rate may depend on the land preparation method for this small‐seeded crop, a study was conducted to evaluate the effect of tillage system (conventional, no‐till, broadcast‐disc, and ripper‐roller) and seeding rate (1.12, 5.60, 10.09, and 14.57 kg seed ha⁻¹) on the performance of B. carinata. A randomized complete block design with a strip‐plot restriction on randomization and four replications was implemented in Headland, AL, Jay, FL, and Quincy, FL, over five site‐years during the 2017–2018 and 2018–2019 growing seasons. Data were collected on soil residue cover; plant population; soil penetrometer resistance and moisture; biomass (including carbon and nitrogen); stalk diameter; yield and yield components; seed oil, protein, and glucosinolates concentration; and oil composition. Soil penetrometer resistance was significantly affected by tillage system, with the ripper‐roller consistently having the lowest penetration resistance values across all site‐years. Ripper‐roller tillage had the highest oil content and lowest protein and glucosinolate contents. Yield response to tillage system was variable. Among seeding rate treatments, yield was lowest at 1.12 kg seed ha⁻¹ and similar among 5.60, 10.09, and 14.57 kg seed ha⁻¹ at all site‐years. There was no tillage by seeding rate interaction for yield. Results indicate that among seeding rate treatments used, 5.6 kg seed ha⁻¹ rate was optimal at all site‐years regardless of land preparation method and is thus the recommended seeding rate for commercial carinata production in the Southeastern United States.

The identification of alternate hosts that can act as virus inoculum sources and vector reservoirs in the landscape is critical to understanding virus epidemics. Cotton leafroll dwarf virus (CLRDV) is a serious pathogen in cotton production and is transmitted by the cotton/melon aphid, Aphis gossypii, in a persistent, circulative, and non-propagative manner. CLRDV was first reported in the United States in Alabama in 2017, and thereafter in several cotton-producing states. CLRDV has since established itself in the southeastern United States. The role of alternate hosts in CLRDV establishment is not clear. Fourteen common plant species in the landscape, including crops, weeds, and ornamentals (cotton, hollyhock, marshmallow, country mallow, abutilon, arrowleaf sida, okra, hibiscus, squash, chickpea, evening primrose, henbit, Palmer amaranth, and prickly sida) were tested as potential alternate hosts of CLRDV along with an experimental host (Nicotiana benthamiana) via aphid-mediated transmission assays. CLRDV was detected following inoculation in hibiscus, okra, N. benthamiana, Palmer amaranth, and prickly sida by RT-PCR, but not in the others. CLRDV accumulation determined by RT-qPCR was the highest in N. benthamiana compared with cotton and other hosts. However, aphids feeding on CLRDV-infected prickly sida, hibiscus, and okra alone were able to acquire CLRDV and back-transmit it to non-infected cotton seedlings. Additionally, some of the alternate CLRDV hosts supported aphid development on par with cotton. However, in a few instances, aphid fitness was reduced when compared with cotton. Overall, this study demonstrated that plant hosts in the agricultural landscape can serve as CLRDV inoculum sources and as aphid reservoirs and could possibly play a role in the reoccurring epidemics of CLRDV in the southeastern United States.