With the continuous expansion of maritime trade scale, ports have put forward increasingly higher requirements for transshipment efficiency. Container twist lock disassembly is a key link in the loading and unloading process, and its automation level has a significant impact on the ship’s berthing time at the port. Aiming at the demand of automated disassembly for high-precision 3D vision, this paper proposes a multi-view point cloud local registration method for twist lock recognition. First, Hierarchical Density-Based Spatial Clustering of Applications with Noise (HDBSCAN) is used to extract the keyhole region with the highest overlap in multi-view point clouds, reducing the interference from non-overlapping structures. Then, a two-stage strategy of “coarse registration + fine registration” is adopted: initial alignment is achieved through Random Sample Consensus (RANSAC), and the Iterative Closest Point (ICP) algorithm is improved by combining adaptive distance threshold and normal consistency constraint to complete fine registration. Experimental results show that the proposed method outperforms the global registration scheme in both accuracy and efficiency: the Root Mean Square Error (RMSE) is reduced to 2.15 mm, the Relative Mean Distance (RMD) is reduced to 1.81 mm, and the registration time is approximately 2.41 s. Compared with global registration, the efficiency is improved by 44.2%, which can meet the real-time requirements of continuous operation at automated terminals for the perception link and the time constraints for subsequent manipulator control. The research results preliminarily verify the application potential of this method in the scenario of automated twist lock disassembly.