Background: This study aimed to compare psychomotor performance between regular League of Legends players and control participants. Methods: This cross-sectional study included 71 adults aged 18 to 30 years, divided into a player group, n = 35, and a control group, n = 36. Eleven outcomes assessing visual reaction time, target acquisition, response speed, accuracy, synchronization, and bilateral hand–eye coordination were evaluated using browser-based tasks and CogniFit assessments. Between-group differences were examined using Welch’s t-test with Holm adjustment for multiple comparisons. Mann–Whitney tests were conducted as sensitivity analyses. Results: After making the adjustments, nine outcomes remained significant. Players showed faster visual reaction times using their right hand (mean difference = −45.33 ms, 95% CI [−60.55, −30.12], d = −1.41) and left hand (−47.67 ms, [−65.09, −30.25], d = −1.29), quicker target acquisition with the right hand (−116.68 ms per target, [−153.69, −79.67], d = −1.49) and left hand (−156.01 ms per target, [−227.35, −84.67], d = −1.03), and shorter response times on the Simon Task (−0.107 s, [−0.153, −0.062], d = −1.12). They also obtained higher scores on HECOOR (1.11%, [0.28, 1.94], d = 0.63), UPDA-SHIF (0.44%, [0.13, 0.76], d = 0.66), and bilateral hand–eye coordination. Conclusions: These results indicate that League of Legends players exhibit better psychomotor skills on tasks requiring quick visual responses and good hand–eye coordination. Still, because this study used a cross-sectional design, we cannot draw causal conclusions. More research is needed to confirm these specific effects.