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Excess hepatic triglyceride (TG) accumulation (steatosis) commonly observed in obesity, may lead to non-alcoholic fatty liver disease (NAFLD). Altered regulation of intracellular lipid droplets (LD) and TG metabolism, as well as activation of JNK-mediated proinflammatory pathways may trigger liver steatosis-related disorders. Drosophila melanogaster is an animal model used for studying obesity and its associated disorders. In Drosophila, lipids and glycogen are stored in the fat body (FB), which resembles mammalian adipose tissue and liver. Dietary oversupply leads to obesity-related disorders, which are characterized by FB dysfunction. Infusions of Lampaya medicinalis Phil. (Verbenaceae) are used in folk medicine of Chile to counteract inflammatory diseases. Hydroethanolic extract of lampaya (HEL) contains considerable amounts of flavonoids that may explain its anti-inflammatory effect. We studied whether HEL affects palmitic acid (PA, C16:0) and oleic acid (OA; C18:1)-induced TG accumulation and proinflammatory marker content in HepG2 hepatocytes as well as impaired lipid storage and proinflammatory molecule expression in Drosophila melanogaster fed a high-fat diet (HFD). In HepG2 hepatocytes, exposure to OA/PA elevated TG content, FABP4, ATGL and DGAT2 expression, and the JNK proinflammatory pathway, as well as TNF-α and IL-6 production, while diminished FAS expression. These effects were prevented by HEL co-treatment. In Drosophila larvae fed a HFD, HEL prevented TG accumulation and downregulated proinflammatory JNK pathway activation. HEL effect counteracting OA/PA- and HFD-induced lipid accumulation and proinflammatory marker expression in HepG2 hepatocytes and Drosophila larvae may represent a preventive approach against hepatic steatosis and inflammation, associated to obesity and NAFLD.
Sofía Sanhueza; Nicolás Tobar; Mariana Cifuentes; Daniela Quenti; Rosaria Varì; Beatrice Scazzocchio; Roberta Masella; Karin Herrera; Adrián Paredes; Glauco Morales; Paulina Ormazabal. Lampaya Medicinalis Phil. decreases lipid-induced triglyceride accumulation and proinflammatory markers in human hepatocytes and fat body of Drosophila melanogaster. International Journal of Obesity 2021, 45, 1464 -1475.
AMA StyleSofía Sanhueza, Nicolás Tobar, Mariana Cifuentes, Daniela Quenti, Rosaria Varì, Beatrice Scazzocchio, Roberta Masella, Karin Herrera, Adrián Paredes, Glauco Morales, Paulina Ormazabal. Lampaya Medicinalis Phil. decreases lipid-induced triglyceride accumulation and proinflammatory markers in human hepatocytes and fat body of Drosophila melanogaster. International Journal of Obesity. 2021; 45 (7):1464-1475.
Chicago/Turabian StyleSofía Sanhueza; Nicolás Tobar; Mariana Cifuentes; Daniela Quenti; Rosaria Varì; Beatrice Scazzocchio; Roberta Masella; Karin Herrera; Adrián Paredes; Glauco Morales; Paulina Ormazabal. 2021. "Lampaya Medicinalis Phil. decreases lipid-induced triglyceride accumulation and proinflammatory markers in human hepatocytes and fat body of Drosophila melanogaster." International Journal of Obesity 45, no. 7: 1464-1475.
Cryptocarya alba (Peumo; CA) and Laurelia sempervirens (Laurel; LS) are herbs native to the Chilean highlands and have historically been used for medicinal purposes by the Huilliches people. In this work, the essential oils were extracted using hydrodistillation in Clevenger apparatus and analyzed by GC-MS to determine their composition. The antioxidant capacity (AC) was evaluated in vitro. The cytotoxicity was determined using cell line cultures both non tumoral and tumoral. The toxicity was determined using the nematode Caenorhabditis elegans. The antimicrobial activity was evaluated against 52 bacteria using the agar disc diffusion method and the minimum inhibitory concentrations (MICs) were determined. The principal compounds found in C. alba essential oil (CA_EO) were α-terpineol (24.96%) and eucalyptol (21.63%) and were isazafrol (91.9%) in L. sempervirens essential oil (LS_EO). Both EOs showed antioxidant capacity in vitro. Both EO showed antibacterial activity against bacteria using. LS_EO showed more inhibitory effect on these cell lines respect to CA_EO. Both EOs showed toxicity against the nematode C.elegans at 3.12–50 mg/mL. The essential oils of CA and LS have an important bioactive potential in their antioxidant, antibacterial and cytotoxicity activity. Both essential oils could possibly be used in the field of natural medicine, natural food preservation, cosmetics, sanitation and plaguicides among others.
Jorge Touma; Myriam Navarro; Betsabet Sepúlveda; Alequis Pavon; Gino Corsini; Katia Fernández; Claudia Quezada; Angelo Torres; María José Larrazabal-Fuentes; Adrian Paredes; Ivan Neira; Matías Ferrando; Flavia Bruna; Alejandro Venegas; Jessica Bravo. The Chemical Compositions of Essential Oils Derived from Cryptocarya alba and Laurelia sempervirens Possess Antioxidant, Antibacterial and Antitumoral Activity Potential. Molecules 2020, 25, 5600 .
AMA StyleJorge Touma, Myriam Navarro, Betsabet Sepúlveda, Alequis Pavon, Gino Corsini, Katia Fernández, Claudia Quezada, Angelo Torres, María José Larrazabal-Fuentes, Adrian Paredes, Ivan Neira, Matías Ferrando, Flavia Bruna, Alejandro Venegas, Jessica Bravo. The Chemical Compositions of Essential Oils Derived from Cryptocarya alba and Laurelia sempervirens Possess Antioxidant, Antibacterial and Antitumoral Activity Potential. Molecules. 2020; 25 (23):5600.
Chicago/Turabian StyleJorge Touma; Myriam Navarro; Betsabet Sepúlveda; Alequis Pavon; Gino Corsini; Katia Fernández; Claudia Quezada; Angelo Torres; María José Larrazabal-Fuentes; Adrian Paredes; Ivan Neira; Matías Ferrando; Flavia Bruna; Alejandro Venegas; Jessica Bravo. 2020. "The Chemical Compositions of Essential Oils Derived from Cryptocarya alba and Laurelia sempervirens Possess Antioxidant, Antibacterial and Antitumoral Activity Potential." Molecules 25, no. 23: 5600.
Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.
Roberto Ybañez-Julca; Daniel Asunción-Alvarez; Ivan Quispe-Díaz; Javier Palacios; Jorge Bórquez; Mario Simirgiotis; Shagufta Perveen; Chukwuemeka Nwokocha; Fredi Cifuentes; Adrián Paredes. Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models. Molecules 2020, 25, 5149 .
AMA StyleRoberto Ybañez-Julca, Daniel Asunción-Alvarez, Ivan Quispe-Díaz, Javier Palacios, Jorge Bórquez, Mario Simirgiotis, Shagufta Perveen, Chukwuemeka Nwokocha, Fredi Cifuentes, Adrián Paredes. Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models. Molecules. 2020; 25 (21):5149.
Chicago/Turabian StyleRoberto Ybañez-Julca; Daniel Asunción-Alvarez; Ivan Quispe-Díaz; Javier Palacios; Jorge Bórquez; Mario Simirgiotis; Shagufta Perveen; Chukwuemeka Nwokocha; Fredi Cifuentes; Adrián Paredes. 2020. "Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models." Molecules 25, no. 21: 5149.
Vasomotion is defined as rhythmic oscillations in arterial diameter which regulate the blood flow and blood pressure. Since antitumor treatment may impair vascular functions and increase the blood pressure, we sought to evaluate whether a new naphthoquinone derivative, postulated as antitumor agent, manifests adverse effects on vascular function. Herein, we evaluated the toxicity of 2-(4-hydroxyphenyl) amino-1,4-naphthoquinone (Q7) and its effects on vascular vasomotion in three models of vascular structure; endothelial cells, aortic ring and smooth muscle cells. While showing notoxic effects, Q7 inhibited the formation of capillary-like structures of the EA.hy926 endothelial cell line grown on Matrigel. In ex-vivo experiments with aortic rings pre-contracted with phenylephrine (PE, 10-6 M), Q7 (10-5 M) significantly (p <0.05) reduced vascular rhythmic contractions induced by the acetylcholine (ACh; 10-7-10-5 M), while sodium nitroprusside (a nitric oxide donor; 10-8 M) recovered the vasomotion. Furthermore, Q7 (10-5 M) did not decrease KCl-induced vascular rhythmic contractions in aortic rings pre-contracted with BaCl2 (a non-selective blocker K+ channels; 10-3 M). Vascular smooth muscle cells (A7r5) pre-incubated with Q7 (10-5 M) for 3 h also demonstrated reduced glucose uptake. However, ATP content was unaffected, suggesting that the rapid reduction in vasomotion observed in vascular reactivity experiments did not involve cellular metabolism, but may be due to faster mechanisms involving endothelial nitric oxide (NO) and K+ channels leading to oscillations in intracellular Ca2+. In summary, the naphthoquinone derivative Q7 presents low cytotoxicity yet may alter endothelial cell response and vasomotion in the absence of changes in smooth muscle cell metabolism.
Javier Palacios; Julio Benites; Gareth I. Owen; Pablo Morales; Mario Chiong; Chukwuemeka R. Nwokocha; Adrián Paredes; Fredi Cifuentes. Impact of the Potential Antitumor Agent 2-(4-Hydroxyphenyl) Amino-1,4-Naphthoquinone (Q7) on Vasomotion Is Mediated by the Vascular Endothelium, But Not Vascular Smooth Muscle Cell Metabolism. Journal of Cardiovascular Pharmacology 2020, 77, 245 -252.
AMA StyleJavier Palacios, Julio Benites, Gareth I. Owen, Pablo Morales, Mario Chiong, Chukwuemeka R. Nwokocha, Adrián Paredes, Fredi Cifuentes. Impact of the Potential Antitumor Agent 2-(4-Hydroxyphenyl) Amino-1,4-Naphthoquinone (Q7) on Vasomotion Is Mediated by the Vascular Endothelium, But Not Vascular Smooth Muscle Cell Metabolism. Journal of Cardiovascular Pharmacology. 2020; 77 (2):245-252.
Chicago/Turabian StyleJavier Palacios; Julio Benites; Gareth I. Owen; Pablo Morales; Mario Chiong; Chukwuemeka R. Nwokocha; Adrián Paredes; Fredi Cifuentes. 2020. "Impact of the Potential Antitumor Agent 2-(4-Hydroxyphenyl) Amino-1,4-Naphthoquinone (Q7) on Vasomotion Is Mediated by the Vascular Endothelium, But Not Vascular Smooth Muscle Cell Metabolism." Journal of Cardiovascular Pharmacology 77, no. 2: 245-252.
Background: Obesity is a major health problem associated with increased comorbidities, which are partially triggered by inflammation. Proinflammatory macrophage infiltration in adipose tissue of individuals with obesity increases chronic inflammation. Obesity is associated with elevated plasma levels of saturated fatty acids, such as palmitic acid (PA), which promotes inflammation in vivo and in vitro. Infusions of Lampaya medicinalis Phil. (Verbenaceae) are used in the folk medicine of Northern Chile to counteract inflammation of rheumatic diseases. Hydroethanolic extract of lampaya (HEL) contains spectrophotometrically defined compounds that may contribute to the observed effect on inflammation. Methods: We evaluated the phytochemical composition of HEL by high-performance liquid chromatography coupled to diode array detection (HPLC-DAD) and liquid chromatography-electrospray ionization- tandem mass spectrometry (LC-ESI-MS/MS). We assessed whether the exposure to HEL affects PA-induced expression of proinflammatory factors in THP-1 macrophages. Results: HPLC-DAD and LC-ESI-MS/MS analyses showed the presence of considerable amounts of flavonoids in HEL. The PA-induced phosphorylation of the inflammatory pathway mediators IKK and NF-κB, as well as the elevated expression and secretion of proinflammatory cytokines (IL-6, TNF-α), were reduced in cells pre-exposed to HEL. Conclusion: These findings give new insights about the effect of HEL reducing IKK/NF-κB proinflammatory pathway, likely explained by the number of flavonoids contained in the extract. More studies would be needed to define the possible role of Lampaya as a preventive approach in subjects with obesity whose circulating PA might contribute to chronic inflammation.
Paulina Ormazabal; Mariana Cifuentes; Rosaria Varì; Beatrice Scazzocchio; Roberta Masella; Igor Pacheco; Wladimir Vega; Adrián Paredes; Glauco Morales. Hydroethanolic Extract of Lampaya Medicinalis Phil. (Verbenaceae) Decreases Proinflammatory Marker Expression in Palmitic Acid-exposed Macrophages. Endocrine, Metabolic & Immune Disorders - Drug Targets 2020, 20, 1309 -1320.
AMA StylePaulina Ormazabal, Mariana Cifuentes, Rosaria Varì, Beatrice Scazzocchio, Roberta Masella, Igor Pacheco, Wladimir Vega, Adrián Paredes, Glauco Morales. Hydroethanolic Extract of Lampaya Medicinalis Phil. (Verbenaceae) Decreases Proinflammatory Marker Expression in Palmitic Acid-exposed Macrophages. Endocrine, Metabolic & Immune Disorders - Drug Targets. 2020; 20 (8):1309-1320.
Chicago/Turabian StylePaulina Ormazabal; Mariana Cifuentes; Rosaria Varì; Beatrice Scazzocchio; Roberta Masella; Igor Pacheco; Wladimir Vega; Adrián Paredes; Glauco Morales. 2020. "Hydroethanolic Extract of Lampaya Medicinalis Phil. (Verbenaceae) Decreases Proinflammatory Marker Expression in Palmitic Acid-exposed Macrophages." Endocrine, Metabolic & Immune Disorders - Drug Targets 20, no. 8: 1309-1320.
Acantholippia deserticola (Rica-Rica) is a native shrub of the Chilean highlands used as a medicinal plant and food dressing. The objective of this study was to compare the physical, antioxidant and antimicrobial characteristics of its essential oil (EO), based on the process parameters and extraction methods using hydrodistillation (HD) and microwave-assisted hydrodistillation (MAHD), and assessing presence of fatty acids. The process performance as well as the colour parameters, refractive index, fatty acid profile, antioxidant activity and antimicrobial activity were evaluated. The best process conditions for HD were 90 min, 420 W and 150 g 1000 mL–1; for MAHD, these were 90 min, 700 W and 200 g 1000 mL–1, with yields of 0.45 and 0.49 mL 100 g-1 and antioxidant capacities of 2.38 and 3.92 µmol Trolox g-1 respectively. The collection season, extraction method and its parameters affected the yield and fatty acid profile, influencing EOs’ activity. In terms of mass of herbs, process time as well as energy efficiency and environmental impact, the extraction of MAHD was 8% more efficient. EOs extracted by both methods show a slight inhibitory effect on Streptococcus sp. and Bacillus cereus, and MAHD EO showed a highly inhibitory effect on Streptococcus Group A. The type of extraction method and the process parameters could be set to obtain suitable EOs according to its potential industrial application.
Jenifer Palma; Ana Mercado; Adrian Paredes; Catherine Lizama; Gissel Pohl; Maria Larrazabal. Assessing properties of acantholippia deserticola (Phil.) moldenke essential oil: Comparison between hydrodistillation and microwave-assisted hydrodistillation extraction methods. Quality Assurance and Safety of Crops & Foods 2020, 12, 36 -49.
AMA StyleJenifer Palma, Ana Mercado, Adrian Paredes, Catherine Lizama, Gissel Pohl, Maria Larrazabal. Assessing properties of acantholippia deserticola (Phil.) moldenke essential oil: Comparison between hydrodistillation and microwave-assisted hydrodistillation extraction methods. Quality Assurance and Safety of Crops & Foods. 2020; 12 (4):36-49.
Chicago/Turabian StyleJenifer Palma; Ana Mercado; Adrian Paredes; Catherine Lizama; Gissel Pohl; Maria Larrazabal. 2020. "Assessing properties of acantholippia deserticola (Phil.) moldenke essential oil: Comparison between hydrodistillation and microwave-assisted hydrodistillation extraction methods." Quality Assurance and Safety of Crops & Foods 12, no. 4: 36-49.
Heliotropium taltalense is an endemic species of the northern coast of Chile and is used as folk medicine. The polyphenolic composition of the methanolic and aqueous extract of the endemic Chilean species was investigated using Ultrahigh-Performance Liquid Chromatography, Heated Electrospray Ionization and Mass Spectrometry (UHPLC-Orbitrap-HESI-MS). Fifty-three compounds were detected, mainly derivatives of benzoic acid, flavonoids, and some phenolic acids. Furthermore, five major compounds were isolated by column chromatography from the extract, including four flavonoids and one geranyl benzoic acid derivative, which showed vascular relaxation and were in part responsible for the activity of the extracts. Since aqueous extract of H. taltalense (83% ± 9%, 100 μg/mL) produced vascular relaxation through an endothelium-dependent mechanism in rat aorta, and the compounds rhamnocitrin (89% ± 7%; 10−4 M) and sakuranetin (80% ± 6%; 10−4 M) also caused vascular relaxation similar to the extracts of H. taltalense, these pure compounds are, to some extent, responsible for the vascular relaxation.
Ruth E. Barrientos; Mario J. Simirgiotis; Javier Palacios; Adrián Paredes; Jorge Bórquez; Alejandra Bravo; Fredi Cifuentes. Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta. Molecules 2020, 25, 3105 .
AMA StyleRuth E. Barrientos, Mario J. Simirgiotis, Javier Palacios, Adrián Paredes, Jorge Bórquez, Alejandra Bravo, Fredi Cifuentes. Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta. Molecules. 2020; 25 (14):3105.
Chicago/Turabian StyleRuth E. Barrientos; Mario J. Simirgiotis; Javier Palacios; Adrián Paredes; Jorge Bórquez; Alejandra Bravo; Fredi Cifuentes. 2020. "Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta." Molecules 25, no. 14: 3105.
The infusion of the desertic plant Nolana ramosissima I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated from a methanolic extract using high-performance counter-current chromatography (HPCCC). The N. ramosissima extract showed endothelium-independent relaxation effect in rat aorta. Sixty-one compounds were detected in the infusion, mainly glycosylated flavonoids, flavanones and several oxylipins, suggesting that a synergistic effect between the compounds in the extracts could be responsible for the relaxation activity. Vascular activity experiments were done in isolated organ bath. In rat aorta, a nitric oxide inhibitor did not prevent the relaxation effects of the extract; however, a selective guanylyl cyclase inhibitor partially blunted this effect. The compound 5,3′-dihydroxy-4′7-dimethoxyflavone presented higher relaxation effect than 100 μg/mL of N. ramosissima extract. The extract and the isolated metabolites from N. ramosissima can show relaxation effects on rat aorta by a mechanism that is independent of the endothelium.
Fredi Cifuentes; Javier Palacios; Jorge Bórquez; Adrián Paredes; Claudio Parra; Alejandra Bravo; Mario J. Simirgiotis. Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta. Molecules 2020, 25, 520 .
AMA StyleFredi Cifuentes, Javier Palacios, Jorge Bórquez, Adrián Paredes, Claudio Parra, Alejandra Bravo, Mario J. Simirgiotis. Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta. Molecules. 2020; 25 (3):520.
Chicago/Turabian StyleFredi Cifuentes; Javier Palacios; Jorge Bórquez; Adrián Paredes; Claudio Parra; Alejandra Bravo; Mario J. Simirgiotis. 2020. "Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta." Molecules 25, no. 3: 520.
Parastrephia quadrangularis (Pq), commonly called “Tola”, is widely used in folk medicine in the Andes, including for altitude sickness. In this study, polyphenolic composition was determined, and hypotensive effects were measured; the ethnopharmacological use as hypotensive was related to the presence of phenolic compounds. For this purpose, male Sprague-Dawley rats (6 to 8 weeks of age, 160 to 190 g) were fed Pq extract (10 to 40 mg/kg) for 10 days through gavage. Blood pressures and heart rate were significantly (p < 0.01) reduced in normotensive rats receiving Pq extract (40 mg/kg body weight). Pq extract induced a negative inotropic effect, and endothelium-dependent vasodilation mediated by nitric oxide (NO). Furthermore, preincubation with Pq extract significantly decreased the cytosolic calcium on vascular smooth muscle cells A7r5 in response to L-phenylephrine (PE). Seven metabolites were isolated from the Pq extract, but three flavonoids (10−4 M) showed similar vasodilation to the extract in intact rat aorta as follows: 5,3′,4′-trihydroxy-7-methoxyflavanone (2); 3,5,4′-trihydroxy-7,8,3′-trimethoxyflavone (6); and 5,4′-dihydroxy-3,7,8,3′-tetramethoxyflavone (7). The Pq extract and compounds 2 and 7 significantly (p < 0.05) reduced the contraction to Bay K8644 (10 nM, an agonist of CaV1.2 channels). Administration of Pq decreased cardiac contractility and increased endothelium-dependent and -independent vasodilation.
Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha; Jorge Bórquez; Mario J. Simirgiotis; Ignacio Norambuena; Mario Chiong; Adrián Paredes. Polyphenolic Composition and Hypotensive Effects of Parastrephia quadrangularis (Meyen) Cabrera in Rat. Antioxidants 2019, 8, 591 .
AMA StyleFredi Cifuentes, Javier Palacios, Chukwuemeka R. Nwokocha, Jorge Bórquez, Mario J. Simirgiotis, Ignacio Norambuena, Mario Chiong, Adrián Paredes. Polyphenolic Composition and Hypotensive Effects of Parastrephia quadrangularis (Meyen) Cabrera in Rat. Antioxidants. 2019; 8 (12):591.
Chicago/Turabian StyleFredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha; Jorge Bórquez; Mario J. Simirgiotis; Ignacio Norambuena; Mario Chiong; Adrián Paredes. 2019. "Polyphenolic Composition and Hypotensive Effects of Parastrephia quadrangularis (Meyen) Cabrera in Rat." Antioxidants 8, no. 12: 591.
Haematococcus pluvialis is known to be a natural source of antioxidants for numerous applications. In this study, an oleoresin rich in carotenoids extracted by supercritical CO2 treatment of H. pluvialis was extensively characterized for its antioxidant capacity. Carotenoid content, fatty acid profile, total phenol content, antioxidant capacity, and viscosity of the oleoresin were determined with the aim of ascertaining the potential of the oleoresin in terms of its antioxidant content for food applications. The oleoresin contained 96.22 mg/g of total astaxanthin (which includes free astaxanthin and astaxanthin esters) and mostly included unsaturated fatty acids (~78% of total fatty acids). High total phenol content and ferric reducing antioxidant potential indicated high antioxidant capacity, but oxygen radical absorbance capacity was lower compared to the oleoresin samples obtained from other species. The oleoresin was a non-Newtonian fluid since it had shear-thinning (pseudoplastic) and shear-thickening (dilatant) flow. Therefore, the H. pluvialis oleoresin is a potential alternative in developing functional ingredients for designing healthy food products. To the best of our knowledge, this is the first study that has reported an extensive characterization of the antioxidant properties of a microalgal oleoresin obtained by means of supercritical CO2 fluid extraction.
Mari Carmen Ruiz-Domínguez; Carolina Espinosa; Adrián Paredes; Jenifer Palma; Carolina Jaime; Carlos Vílchez; Pedro Cerezal. Determining the Potential of Haematococcus pluvialis Oleoresin as a Rich Source of Antioxidants. Molecules 2019, 24, 4073 .
AMA StyleMari Carmen Ruiz-Domínguez, Carolina Espinosa, Adrián Paredes, Jenifer Palma, Carolina Jaime, Carlos Vílchez, Pedro Cerezal. Determining the Potential of Haematococcus pluvialis Oleoresin as a Rich Source of Antioxidants. Molecules. 2019; 24 (22):4073.
Chicago/Turabian StyleMari Carmen Ruiz-Domínguez; Carolina Espinosa; Adrián Paredes; Jenifer Palma; Carolina Jaime; Carlos Vílchez; Pedro Cerezal. 2019. "Determining the Potential of Haematococcus pluvialis Oleoresin as a Rich Source of Antioxidants." Molecules 24, no. 22: 4073.
Acantholippia deserticola (Rica rica; RR) and Artemisia copa (Copa copa; CC) are herbs native to the Chilean highlands and have historically been used for medicinal purposes by the Atacameños people. The essential oils were analized by GC/MS, the principal compounds found in RR essential oil (RR_EO) were β-thujone (45%), β-linalool (16%), and eucalyptol (9%) and were butyric acid (38%), γ-terpinene (16%), and cis-2-menthenol (12%) in CC essential oil (CC_EO). The total polyphenol content (TPC) and the antioxidant capacity (AC) were evaluated. The TPC was 25 and 20 mg/mL for CC_EO and RR_EO, respectively, and the respective AC values were 740 and 320 μg eq Trolox/mL by the ferric reducing antioxidant power (FRAP) method. The IC50 (1,1-diphenyl-2-picrylhydrazyl; DPPH) values were 316.9 and 485.8 μg/mL for CC_EO and RR_EO, respectively. Antimicrobial activity was evaluated against 32 bacteria using the agar disc diffusion method. CC_EO exerted a significant inhibitory effect (>20 mm) against 9 bacteria, including Listeria monocytogenes and Staphylococcus aureus, and RR_EO significantly inhibited Escherichia coli. The CC_EO MIC values were 0.39 μg/mL for Corynebacterium. The RR_EO MIC value was 6.25 μg/mL for S. viridans. Neither EO showed toxicity against the nematode C. elegans at 2.5 mg/mL. The essential oils of RR and CC, extracted by MAHD have important bioactive potential and low toxicity, which would allow their use of active ingredients that could potentially be used in areas such as the clinic, food, cosmetics, sanitizers, and detergents, among others.
María Larrazabal-Fuentes; Jenifer Palma; Adrián Paredes; Ana Mercado; Iván Neira; Catherine Lizama; Bestabet Sepulveda; Jessica Bravo. Chemical composition, antioxidant capacity, toxicity and antibacterial activity of the essential oils from Acantholippia deserticola (Phil.) Moldenke (Rica rica) and Artemisia copa Phil. (Copa copa) extracted by microwave-assisted hydrodistillation. Industrial Crops and Products 2019, 142, 111830 .
AMA StyleMaría Larrazabal-Fuentes, Jenifer Palma, Adrián Paredes, Ana Mercado, Iván Neira, Catherine Lizama, Bestabet Sepulveda, Jessica Bravo. Chemical composition, antioxidant capacity, toxicity and antibacterial activity of the essential oils from Acantholippia deserticola (Phil.) Moldenke (Rica rica) and Artemisia copa Phil. (Copa copa) extracted by microwave-assisted hydrodistillation. Industrial Crops and Products. 2019; 142 ():111830.
Chicago/Turabian StyleMaría Larrazabal-Fuentes; Jenifer Palma; Adrián Paredes; Ana Mercado; Iván Neira; Catherine Lizama; Bestabet Sepulveda; Jessica Bravo. 2019. "Chemical composition, antioxidant capacity, toxicity and antibacterial activity of the essential oils from Acantholippia deserticola (Phil.) Moldenke (Rica rica) and Artemisia copa Phil. (Copa copa) extracted by microwave-assisted hydrodistillation." Industrial Crops and Products 142, no. : 111830.
Alkaloids derived from plants have shown great medicinal benefits, and are often reported for their use in cardiovascular disease management. Aristotelia chilensis (Molina) Stuntz (Maqui) has shown important medicinal properties in traditional useage. In this study, we evaluated the effect of the indole-alkaloid aristoteline (ARI), isolated from leaves of Maqui, on vascular reactivity of isolated aortic rings from normotensive rats. ARI induced relaxation (100%) in a concentration-dependent manner in intact or denuded-endothelium aortic rings pre-contracted with phenylephrine (PE; 1 μM). However, a specific soluble guanylyl cyclase inhibitor (ODQ; 1 μM) significantly reduced the relaxation to ARI in aortic rings pre-contracted with PE. In the presence of ARI, the contraction induced by KCl or PE was significantly (p < 0.05) decreased. Interestingly, the potassium channel blockade with 10 μM BaCl2 (Kir), 10 μM glibenclamide (KATP), 1 mM tetraethylammonium (TEA; KCa1.1), or 1 mM 4-aminopyridine (4-AP; Kv) significantly (p < 0.05) reduced the ARI-induced relaxation. ARI significantly (p < 0.05) reduced the contractile response to agonist of CaV1.2 channels (Bay K8644; 10 nM), likely reducing the influx of extracellular calcium through plasma membrane. The mechanisms associated with this process suggest an activation of the potassium channels, a calcium-induced antagonism and endothelium independent vasodilation that possibly involves the nitric oxide-independent soluble guanylate cyclase pathway.
Fernando Romero; Javier Palacios; Ignacio Jofré; Cristian Paz; Chukwuemeka R. Nwokocha; Adrián Paredes; Fredi Cifuentes. Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta. Molecules 2019, 24, 2748 .
AMA StyleFernando Romero, Javier Palacios, Ignacio Jofré, Cristian Paz, Chukwuemeka R. Nwokocha, Adrián Paredes, Fredi Cifuentes. Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta. Molecules. 2019; 24 (15):2748.
Chicago/Turabian StyleFernando Romero; Javier Palacios; Ignacio Jofré; Cristian Paz; Chukwuemeka R. Nwokocha; Adrián Paredes; Fredi Cifuentes. 2019. "Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta." Molecules 24, no. 15: 2748.
Guinep is traditionally used in the management of cardiovascular ailments. This study aims to evaluate its medicinal constituents and effects in the management of myocardial injury in an experimental isoproterenol (ISO) rat model. Sprague-Dawley rats were randomly assigned to four groups: Group 1 was the control group; Group 2 received M. bijugatus extract (100 mg/Kg; MB) for six weeks; Group 3 was given ISO (85 mg/Kg) i.p. twice during a 24-hour period; and Group 4 was given ISO (85 mg/Kg) i.p. and MB extract (100 mg/Kg) for six weeks. The MB was administered orally by gavage, daily. The blood pressure of conscious animals was measured, while ECG was performed under anesthesia. Blood and serum were collected for biochemical and hematological analysis. The ISO group treated with MB showed a significant decrease (p < 0.001) in (SBP), diastolic (DBP), mean arterial (MAP) and heart rate (HR) compared to the ISO only group. Conversely, MB treated rats that were not induced with ISO displayed a significant decreases (p < 0.001) in SBP, DBP, MAP, and HR. ISO significantly elevated the ST segment (p < 0.001) and shortened the QTc interval (p < 0.05), which were recovered after treatment with 100 mg/Kg of MB. In addition, the results showed a significant decrease (p < 0.001) in the heart to body weight ratio of the ISO group treated with MB compared to the ISO only group. Furthermore, the extract normalized the hematological values depressed by the ISO while significantly elevating the platelet count. UHPLC high-resolution orbitrap mass spectrometry analysis results revealed the presence of several antioxidants like vitamin C and related compounds, phenolic acids, flavonoid, fatty acids (oxylipins), and terpene derivatives. The results of this study indicated that Melicoccus bijugatus did display some cardio-protective effects in relation to myocardial injury.
Chukwuemeka R. Nwokocha; Isheba Warren; Javier Palacios; Mario Simirgiotis; Magdalene Nwokocha; Sharon Harrison; Rory Thompson; Adrian Paredes; Jorge Bórquez; Astrid Lavado; Fredi Cifuentes. Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry. Molecules 2019, 24, 235 .
AMA StyleChukwuemeka R. Nwokocha, Isheba Warren, Javier Palacios, Mario Simirgiotis, Magdalene Nwokocha, Sharon Harrison, Rory Thompson, Adrian Paredes, Jorge Bórquez, Astrid Lavado, Fredi Cifuentes. Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry. Molecules. 2019; 24 (2):235.
Chicago/Turabian StyleChukwuemeka R. Nwokocha; Isheba Warren; Javier Palacios; Mario Simirgiotis; Magdalene Nwokocha; Sharon Harrison; Rory Thompson; Adrian Paredes; Jorge Bórquez; Astrid Lavado; Fredi Cifuentes. 2019. "Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry." Molecules 24, no. 2: 235.
8-Oxo-9-dihydromakomakine is a tetracyclic indole alkaloid extracted from leaves of the Chilean tree Aristotelia chilensis. The present study investigated the effects of this alkaloid on vascular response in tissues isolated from aortic segments obtained from normotensive rats. Our results showed that 8-oxo-9-dihydromakomakine induced a dose-dependent relaxation of aortic rings pre-contracted with phenylephrine (PE; 10−6 M). The vasorelaxation induced by 8-oxo-9-dihydromakomakine in rat aortic rings is independent of endothelium. The pre-incubation of aortic rings with 8-oxo-9-dehydromakomakine (10−4 M) significantly reduced the contractile response to KCl (p < 0.001) more than PE (p < 0.05). The highest dose of 8-oxo-9-dehydromakomakine (10−4 M) drastically reduced the contraction to KCl (6·10−2 M), but after that, PE (10−6 M) caused contraction (p < 0.05) in the same aortic rings. The addition of 8-oxo-9-dihydromakomakine (10−5 M) decreased the contractile response to tetraethylammonium (a voltage-dependent potassium channels blocker; TEA; 5 × 10−3 M; p < 0.01) and BaCl2 (a non-selective inward rectifier potassium channel blocker; 5 × 10−3 M; p < 0.001) in rat aorta. 8-oxo-9-dihydromakomakine (10−5 M) decreased the contractile response to PE in rat aorta in the presence or absence of ouabain (an inhibitor of Na,K-ATPase; 10−3 M; p < 0.05). These results could indicate that 8-oxo-9-dihydromakomakine partially reduces plasma membrane depolarization-induced contraction. In aortic rings depolarized by PE, 8-oxo-9-dihydromakomakine inhibited the contraction induced by the influx of extracellular Ca2+ in a Ca2+ free solution (p < 0.01). 8-oxo-9-dihydromakomakine reduced the contractile response to agonists of voltage-dependent calcium channels type L (Bay K6844; 10−8 M; p < 0.01), likely decreasing the influx of extracellular Ca2+ through the voltage-dependent calcium channels. This study provides the first qualitative analysis indicating that traditional folk medicine Aristotelia chilensis may be protective in the treatment of cardiovascular pathologies.
Fredi Cifuentes; Javier Palacios; Adrián Paredes; Chukwuemeka R. Nwokocha; Cristian Paz. 8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx. Molecules 2018, 23, 3050 .
AMA StyleFredi Cifuentes, Javier Palacios, Adrián Paredes, Chukwuemeka R. Nwokocha, Cristian Paz. 8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx. Molecules. 2018; 23 (11):3050.
Chicago/Turabian StyleFredi Cifuentes; Javier Palacios; Adrián Paredes; Chukwuemeka R. Nwokocha; Cristian Paz. 2018. "8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx." Molecules 23, no. 11: 3050.
Xenophyllum poposum is an endemic species of the Andes Cordillera, popularly known as Popusa. Popusa is widely used by mountain communities as a folk medicine to treat altitude sickness and hypertension. The aim of this study is to evaluate the hypotensive effects and vascular reactivity of Popusa extracts and its pure isolated compounds. Hydroalcoholic extract of Xenophyllum poposum (HAE X. poposum; 40 mg/kg dose) were administered to rats by gavage and mean arterial pressures were recorded. Organ bath studies were conducted in endothelium-intact and denuded rings, and the vascular reactivity of the HAE X. poposum extract and its isolated compounds were compared and analysed. Cytosolic Ca2+ was measured in vascular smooth muscle cell line A7r5 using Fura2-AM. HAE X. poposum significantly reduced the mean arterial blood pressure and heart rate in normotensive rats chronically treated with the extract, as well as mice acutely treated with the extract. A negative chronotropic effect was observed in the isolated rat heart. HAE X. poposum induced endothelial vasodilation mediated by nitric oxide (NO), reduced the contractile response to PE, and decreased PE-induced intracellular Ca2+ influx in vascular smooth muscle cells. Pure compounds isolated from HAE X. poposum such as 4-hydroxy-3-(3-methyl-2-butenyl) acetophenone, 5-acetyl-6-hydroxy-2-isopropenyl-2, and 3-dihydrobenzofurane (dihydroeuparin) also triggered endothelium-dependent vasodilation. HAE X. poposum decreases blood pressure, heart rate and vascular response. The vasodilation properties of HAE X. poposum extract and its isolated compounds may act through the endothelial nitric oxide synthase, as well as calcium channel blocker mechanisms. The results of the present study provide the first qualitative analysis that supports the use of X. poposum in traditional folk medicine for the treatment of altitude sickness and hypertension.
Fredi Cifuentes; Javier Palacios; Jovan Kuzmicic; Lorena Carvajal; Fernanda Muñoz; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Ignacio Norambuena-Soto; Mario Chiong; Adrian Paredes. Vasodilator and hypotensive effects of pure compounds and hydroalcoholic extract of Xenophyllum poposum (Phil) V.A Funk (Compositae) on rats. Phytomedicine 2018, 50, 99 -108.
AMA StyleFredi Cifuentes, Javier Palacios, Jovan Kuzmicic, Lorena Carvajal, Fernanda Muñoz, Cristina Quispe, Chukwuemeka R. Nwokocha, Glauco Morales, Ignacio Norambuena-Soto, Mario Chiong, Adrian Paredes. Vasodilator and hypotensive effects of pure compounds and hydroalcoholic extract of Xenophyllum poposum (Phil) V.A Funk (Compositae) on rats. Phytomedicine. 2018; 50 ():99-108.
Chicago/Turabian StyleFredi Cifuentes; Javier Palacios; Jovan Kuzmicic; Lorena Carvajal; Fernanda Muñoz; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Ignacio Norambuena-Soto; Mario Chiong; Adrian Paredes. 2018. "Vasodilator and hypotensive effects of pure compounds and hydroalcoholic extract of Xenophyllum poposum (Phil) V.A Funk (Compositae) on rats." Phytomedicine 50, no. : 99-108.
Paulina Ormazabal; Mariana Cifuentes; Cecilia Fuentes; Adrián Paredes; Glauco Morales. Effect of the Andean plant Lampaya on NF-κB and JNK phosphorylation in human macrophages treated with palmitic acid. Endocrine Abstracts 2018, 1 .
AMA StylePaulina Ormazabal, Mariana Cifuentes, Cecilia Fuentes, Adrián Paredes, Glauco Morales. Effect of the Andean plant Lampaya on NF-κB and JNK phosphorylation in human macrophages treated with palmitic acid. Endocrine Abstracts. 2018; ():1.
Chicago/Turabian StylePaulina Ormazabal; Mariana Cifuentes; Cecilia Fuentes; Adrián Paredes; Glauco Morales. 2018. "Effect of the Andean plant Lampaya on NF-κB and JNK phosphorylation in human macrophages treated with palmitic acid." Endocrine Abstracts , no. : 1.
Brewing conditions influence the composition and properties of herbal infusions. Acantholippia deserticola (Phil.) (RR) is a native and medicinal, Chilean Altiplano herb. This study assessed the effect of the section plant used, mass:water ratio and brewing time, on the infusions RR characteristics. RR leaves had higher total polyphenols (TPP) and antioxidant capacity (A.C.) than the stem. Polyphenol, chlorophyll and pheophytin extraction was maximized after 5 min brewing. Infusion prepared with 1 g has lower extraction ratio, per herb mass used, than those with 0.5 g. The infusion contained 10.1 µmol/L TPP, 5.5 µg/mL chlorophyll and 4.3 µg/mL pheophytins. Its A.C. was 69.2 µmol Trolox eq./L (FRAP), its IC50 was µg/mL 337 (DPPH) and 438 µg/ml (ABTS), and a dose of 300 mg/kg had an anti-inflammatory effect. The section of the plant, the herb : water ratio and the brewing time affect the characteristics of the RR infusions.
Maria Jose Larrazabal; Jenifer Palma; Adrian Paredes; Glauco Morales; Ana Mercado. Effect of brewing conditions on pigments and total polyphenols content and biological activities of theAcantholippia deserticola (Phil.)infusion. CyTA - Journal of Food 2018, 16, 588 -595.
AMA StyleMaria Jose Larrazabal, Jenifer Palma, Adrian Paredes, Glauco Morales, Ana Mercado. Effect of brewing conditions on pigments and total polyphenols content and biological activities of theAcantholippia deserticola (Phil.)infusion. CyTA - Journal of Food. 2018; 16 (1):588-595.
Chicago/Turabian StyleMaria Jose Larrazabal; Jenifer Palma; Adrian Paredes; Glauco Morales; Ana Mercado. 2018. "Effect of brewing conditions on pigments and total polyphenols content and biological activities of theAcantholippia deserticola (Phil.)infusion." CyTA - Journal of Food 16, no. 1: 588-595.
C15H24O3, orthorhombic, P212121 (no. 19), a = 7.5352(2) Å, b = 10.6639(3) Å, c = 17.5406(5) Å, V = 1409.47(7) Å3, Z = 4, Rgt(F) = 0.0315, wRref(F2) = 0.0838, T = 293 K.
Glauco Morales; Adrián Paredes; Iván Brito; Alejandro Cárdenas. Crystal structure of 1α,11-dihydroxyeremophil-9-en-8-one, C15H24O3. Zeitschrift für Kristallographie - New Crystal Structures 2017, 232, 379 -381.
AMA StyleGlauco Morales, Adrián Paredes, Iván Brito, Alejandro Cárdenas. Crystal structure of 1α,11-dihydroxyeremophil-9-en-8-one, C15H24O3. Zeitschrift für Kristallographie - New Crystal Structures. 2017; 232 (3):379-381.
Chicago/Turabian StyleGlauco Morales; Adrián Paredes; Iván Brito; Alejandro Cárdenas. 2017. "Crystal structure of 1α,11-dihydroxyeremophil-9-en-8-one, C15H24O3." Zeitschrift für Kristallographie - New Crystal Structures 232, no. 3: 379-381.
Oral administration of aqueous extract of Artocarpus artilis has cardio-protective functions in myocardial injury, in part, by decreasing the HR, reduced contractility and infarct size. These findings may explain the cardio-protective use of A. altilis in traditional medicine.
Chukwuemeka Nwokocha; Javier Palacios; Mario J. Simirgiotis; Jemesha Thomas; Magdalene Nwokocha; Lauriann Young; Rory Thompson; Fredi Cifuentes; Adrian Paredes; Rupika Delgoda. Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Negative chronotropic and inotropic effects. Journal of Ethnopharmacology 2017, 203, 163 -170.
AMA StyleChukwuemeka Nwokocha, Javier Palacios, Mario J. Simirgiotis, Jemesha Thomas, Magdalene Nwokocha, Lauriann Young, Rory Thompson, Fredi Cifuentes, Adrian Paredes, Rupika Delgoda. Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Negative chronotropic and inotropic effects. Journal of Ethnopharmacology. 2017; 203 ():163-170.
Chicago/Turabian StyleChukwuemeka Nwokocha; Javier Palacios; Mario J. Simirgiotis; Jemesha Thomas; Magdalene Nwokocha; Lauriann Young; Rory Thompson; Fredi Cifuentes; Adrian Paredes; Rupika Delgoda. 2017. "Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Negative chronotropic and inotropic effects." Journal of Ethnopharmacology 203, no. : 163-170.
HAE S. nutans and its isolated compounds caused vasodilation in rat aorta in absence of endothelium, suggesting its vasodilator properties is endothelium-dependent (NO) and or independent, and may involve a modulation of the calcium channels. This result is of clinical interest as potential therapy control of blood pressure.
Adrian Paredes; Javier Palacios; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Jovan Kuzmicic; Fredi Cifuentes. Hydroalcoholic extract and pure compounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms. Journal of Ethnopharmacology 2016, 192, 99 -107.
AMA StyleAdrian Paredes, Javier Palacios, Cristina Quispe, Chukwuemeka R. Nwokocha, Glauco Morales, Jovan Kuzmicic, Fredi Cifuentes. Hydroalcoholic extract and pure compounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms. Journal of Ethnopharmacology. 2016; 192 ():99-107.
Chicago/Turabian StyleAdrian Paredes; Javier Palacios; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Jovan Kuzmicic; Fredi Cifuentes. 2016. "Hydroalcoholic extract and pure compounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms." Journal of Ethnopharmacology 192, no. : 99-107.