This page has only limited features, please log in for full access.

Dr. Javier Palacios
Universidad Arturo Prat

Basic Info


Research Keywords & Expertise

0 Cardiovascular
0 Hypertension
0 Vascular
0 antioxidant activity
0 medicinal plants

Fingerprints

Vascular
Cardiovascular
Hypertension
antioxidant activity

Honors and Awards

The user has no records in this section


Career Timeline

Facultad Ciencias de la Salud, Universidad Arturo Prat

University Educator/Researcher

01 March 2013 - 01 September 2021




Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 02 April 2021 in Separations
Reads 0
Downloads 0

Periploca aphylla (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use in folk medicine, two different extracts from the aerial parts of the plant: chloroform P-1, and n-butanol P-2 were subjected to biological assays to screen peroxisome proliferator-activated receptors (PPARα and PPARγ) agnostic, anti-inflammatory, antioxidant, cytotoxic, and estrogenic activities. In addition, five bioactive secondary metabolites were isolated from the aerial parts of the plant: rutin, chlorogenic acid, caffeic acid, ursolic acid, and stigmasterol. P-1 and P-2 decreased cellular oxidative stress by 47.0% and 62.0%, respectively, compared to the standard drug quercetin, while one of the compounds rutin PA-1 isolated from P-1 extract and significantly decreased cellular oxidative stress by 67.0% compared to quercetin (75.0%). P-1 and P-2 also significantly activated PPARγ agnostic. P-1 and P-2 did not inhibit nuclear factor kappa B and inducible nitric oxide synthase activity and showed no cytotoxic or estergenic effects on four human cancer cell lines. In this study, both extracts were standardized using high-performance thin-layer chromatography (HPTLC). RP-HPTLC showed sharp and compact bands of rutin (R f = 0.09), caffeic acid (R f = 0.25), and chlorogenic acid (R f = 0.39) scanned at λ max = 340 nm using the water: methanol (60:40 v/v) mobile phase. At λ max = 539 nm ursolic acid (R f = 0.20) and stigmasterol (R f = 0.48) were scanned using the chloroform: methanol (98:2 v/v) as NP-HPTLC mobile phase. Therefore, the developed RP- and NP-HPTLC systems are a precise, sensitive, and specific analytical tool for the quantification of compounds isolated from PA, which can be used as phytomarkers for taxonomical identification and assessment of PA.

ACS Style

Raha Orfali; Shagufta Perveen; Hanan Aati; Perwez Alam; Omar Noman; Javier Palacios; Bayan Al-Kurbi; Areej Al-Taweel; Afsar Khan; Rashad Mehmood; Shabana Khan. High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in Periploca aphylla Extracts. Separations 2021, 8, 44 .

AMA Style

Raha Orfali, Shagufta Perveen, Hanan Aati, Perwez Alam, Omar Noman, Javier Palacios, Bayan Al-Kurbi, Areej Al-Taweel, Afsar Khan, Rashad Mehmood, Shabana Khan. High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in Periploca aphylla Extracts. Separations. 2021; 8 (4):44.

Chicago/Turabian Style

Raha Orfali; Shagufta Perveen; Hanan Aati; Perwez Alam; Omar Noman; Javier Palacios; Bayan Al-Kurbi; Areej Al-Taweel; Afsar Khan; Rashad Mehmood; Shabana Khan. 2021. "High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in Periploca aphylla Extracts." Separations 8, no. 4: 44.

Original article
Published: 11 November 2020 in Reproductive Medicine and Biology
Reads 0
Downloads 0

Purpose Hormone Replacement Therapy (HRT) and herbal remedies are often used to alleviate menopausal symptoms, but their effects and efficacy at high altitudes presents with several uncertainties. The purpose of this study was to evaluate whether pre‐treatment with maca (Lepidium meyenii Walp) improved the tolerance to high altitude on an ovariectomized (OVX) rat model at sea level. Method The animals were treated with 17β‐estradiol (200 µg/kg; E2), red and black maca (1.5 g/kg) for 28 days and exposed at high altitude or sea level. Result Our findings showed that red and black maca extracts significantly (P < .001) reduced the MDA level in OVX rat serum under hypoxia in a similar way to E2. Red and black maca extracts had similar effects with E2, by significantly (P < .001) reversing and increasing the ovariectomized induced decrease in cornified endometrial cell number. Under hypoxic conditions, the black maca (P < .05) and E2 (P < .01) increased the uterine weight in OVX rats. Finally, E2 alone significantly recovered the frequency of the uterine contractile response. Conclusion Aqueous extract of L. meyenii partially protects the reproductive function in hypobaric hypoxic environment, through the recovery of the cornified endometrial cells and uterine weight in a menopausal model of OVX rats.

ACS Style

Roberto O. Ybañez‐Julca; Daniel Asunción‐Alvarez; Javier Palacios; Chukwuemeka R. Nwokocha. Maca extracts and estrogen replacement therapy in ovariectomized rats exposed at high altitude. Reproductive Medicine and Biology 2020, 20, 88 -95.

AMA Style

Roberto O. Ybañez‐Julca, Daniel Asunción‐Alvarez, Javier Palacios, Chukwuemeka R. Nwokocha. Maca extracts and estrogen replacement therapy in ovariectomized rats exposed at high altitude. Reproductive Medicine and Biology. 2020; 20 (1):88-95.

Chicago/Turabian Style

Roberto O. Ybañez‐Julca; Daniel Asunción‐Alvarez; Javier Palacios; Chukwuemeka R. Nwokocha. 2020. "Maca extracts and estrogen replacement therapy in ovariectomized rats exposed at high altitude." Reproductive Medicine and Biology 20, no. 1: 88-95.

Journal article
Published: 05 November 2020 in Molecules
Reads 0
Downloads 0

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

ACS Style

Roberto Ybañez-Julca; Daniel Asunción-Alvarez; Ivan Quispe-Díaz; Javier Palacios; Jorge Bórquez; Mario Simirgiotis; Shagufta Perveen; Chukwuemeka Nwokocha; Fredi Cifuentes; Adrián Paredes. Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models. Molecules 2020, 25, 5149 .

AMA Style

Roberto Ybañez-Julca, Daniel Asunción-Alvarez, Ivan Quispe-Díaz, Javier Palacios, Jorge Bórquez, Mario Simirgiotis, Shagufta Perveen, Chukwuemeka Nwokocha, Fredi Cifuentes, Adrián Paredes. Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models. Molecules. 2020; 25 (21):5149.

Chicago/Turabian Style

Roberto Ybañez-Julca; Daniel Asunción-Alvarez; Ivan Quispe-Díaz; Javier Palacios; Jorge Bórquez; Mario Simirgiotis; Shagufta Perveen; Chukwuemeka Nwokocha; Fredi Cifuentes; Adrián Paredes. 2020. "Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models." Molecules 25, no. 21: 5149.

Journal article
Published: 29 October 2020 in Scientific Reports
Reads 0
Downloads 0

Melicoccus bijugatusJacq (Mb) has been reported to have cardiovascular modulatory effects. In this study, we evaluated the antihypertensive effects and mechanism of action of Mb on NG-Nitro-l-arginine Methyl Ester (l-NAME) and Deoxycorticosterone Acetate (DOCA) rat models. Aqueous extract of Mb fruit (100 mg/kg) was administered for 6 weeks to rats by gavage and blood pressure was recorded. Effects of the extract on vascular reactivity was evaluated using isolated organ baths, and tissues were collected for biochemical and histological analysis. The systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) were significantly (P < 0.05) reduced with extract (100 mg/kg) administration and treatment compared to the hypertensive models. Mb (100 µg/mL) reduced the vascular contractility induced by phenylephrine (PE), and caused a dose-dependent relaxation of PE-induced contraction of aortic vascular rings. The vasorelaxation properties seemed to be endothelium dependent, as well as nitric oxide (NO) and guanylyl cyclase, but not prostaglandin dependent. Histomicrograph of transverse sections of the ventricles from the Mb group did not show abnormalities. The extract significantly (P < 0.05) reduced anl-NAME induced elevation of cardiac output and Creatine Kinase Muscle-Brain (CKMB), but had no significant impact on the activities of arylamineN-acetyltransferase. In conclusion, Mb significantly decreased blood pressure in hypertensive models. The extract possesses the ability to induce endothelium dependent vasodilation, which is dependent on guanylyl cyclase but not prostaglandins.

ACS Style

Chukwuemeka R. Nwokocha; Alexia Gordon; Javier Palacios; Adrian Paredes; Fredi Cifuentes; Sheena Francis; Jeann Watson; Rupika Delgoda; Magdalene Nwokocha; Ruby Alexander-Lindo; Rory Thompson; Donna Minott-Kates; Momoh A. Yakubu. Hypotensive and antihypertensive effects of an aqueous extract from Guinep fruit (Melicoccus bijugatus Jacq) in rats. Scientific Reports 2020, 10, 1 -11.

AMA Style

Chukwuemeka R. Nwokocha, Alexia Gordon, Javier Palacios, Adrian Paredes, Fredi Cifuentes, Sheena Francis, Jeann Watson, Rupika Delgoda, Magdalene Nwokocha, Ruby Alexander-Lindo, Rory Thompson, Donna Minott-Kates, Momoh A. Yakubu. Hypotensive and antihypertensive effects of an aqueous extract from Guinep fruit (Melicoccus bijugatus Jacq) in rats. Scientific Reports. 2020; 10 (1):1-11.

Chicago/Turabian Style

Chukwuemeka R. Nwokocha; Alexia Gordon; Javier Palacios; Adrian Paredes; Fredi Cifuentes; Sheena Francis; Jeann Watson; Rupika Delgoda; Magdalene Nwokocha; Ruby Alexander-Lindo; Rory Thompson; Donna Minott-Kates; Momoh A. Yakubu. 2020. "Hypotensive and antihypertensive effects of an aqueous extract from Guinep fruit (Melicoccus bijugatus Jacq) in rats." Scientific Reports 10, no. 1: 1-11.

Journal article
Published: 08 July 2020 in Molecules
Reads 0
Downloads 0

Heliotropium taltalense is an endemic species of the northern coast of Chile and is used as folk medicine. The polyphenolic composition of the methanolic and aqueous extract of the endemic Chilean species was investigated using Ultrahigh-Performance Liquid Chromatography, Heated Electrospray Ionization and Mass Spectrometry (UHPLC-Orbitrap-HESI-MS). Fifty-three compounds were detected, mainly derivatives of benzoic acid, flavonoids, and some phenolic acids. Furthermore, five major compounds were isolated by column chromatography from the extract, including four flavonoids and one geranyl benzoic acid derivative, which showed vascular relaxation and were in part responsible for the activity of the extracts. Since aqueous extract of H. taltalense (83% ± 9%, 100 μg/mL) produced vascular relaxation through an endothelium-dependent mechanism in rat aorta, and the compounds rhamnocitrin (89% ± 7%; 10−4 M) and sakuranetin (80% ± 6%; 10−4 M) also caused vascular relaxation similar to the extracts of H. taltalense, these pure compounds are, to some extent, responsible for the vascular relaxation.

ACS Style

Ruth E. Barrientos; Mario J. Simirgiotis; Javier Palacios; Adrián Paredes; Jorge Bórquez; Alejandra Bravo; Fredi Cifuentes. Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta. Molecules 2020, 25, 3105 .

AMA Style

Ruth E. Barrientos, Mario J. Simirgiotis, Javier Palacios, Adrián Paredes, Jorge Bórquez, Alejandra Bravo, Fredi Cifuentes. Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta. Molecules. 2020; 25 (14):3105.

Chicago/Turabian Style

Ruth E. Barrientos; Mario J. Simirgiotis; Javier Palacios; Adrián Paredes; Jorge Bórquez; Alejandra Bravo; Fredi Cifuentes. 2020. "Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta." Molecules 25, no. 14: 3105.

Journal article
Published: 24 January 2020 in Molecules
Reads 0
Downloads 0

The infusion of the desertic plant Nolana ramosissima I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated from a methanolic extract using high-performance counter-current chromatography (HPCCC). The N. ramosissima extract showed endothelium-independent relaxation effect in rat aorta. Sixty-one compounds were detected in the infusion, mainly glycosylated flavonoids, flavanones and several oxylipins, suggesting that a synergistic effect between the compounds in the extracts could be responsible for the relaxation activity. Vascular activity experiments were done in isolated organ bath. In rat aorta, a nitric oxide inhibitor did not prevent the relaxation effects of the extract; however, a selective guanylyl cyclase inhibitor partially blunted this effect. The compound 5,3′-dihydroxy-4′7-dimethoxyflavone presented higher relaxation effect than 100 μg/mL of N. ramosissima extract. The extract and the isolated metabolites from N. ramosissima can show relaxation effects on rat aorta by a mechanism that is independent of the endothelium.

ACS Style

Fredi Cifuentes; Javier Palacios; Jorge Bórquez; Adrián Paredes; Claudio Parra; Alejandra Bravo; Mario J. Simirgiotis. Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta. Molecules 2020, 25, 520 .

AMA Style

Fredi Cifuentes, Javier Palacios, Jorge Bórquez, Adrián Paredes, Claudio Parra, Alejandra Bravo, Mario J. Simirgiotis. Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta. Molecules. 2020; 25 (3):520.

Chicago/Turabian Style

Fredi Cifuentes; Javier Palacios; Jorge Bórquez; Adrián Paredes; Claudio Parra; Alejandra Bravo; Mario J. Simirgiotis. 2020. "Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta." Molecules 25, no. 3: 520.

Journal article
Published: 27 November 2019 in Antioxidants
Reads 0
Downloads 0

Parastrephia quadrangularis (Pq), commonly called “Tola”, is widely used in folk medicine in the Andes, including for altitude sickness. In this study, polyphenolic composition was determined, and hypotensive effects were measured; the ethnopharmacological use as hypotensive was related to the presence of phenolic compounds. For this purpose, male Sprague-Dawley rats (6 to 8 weeks of age, 160 to 190 g) were fed Pq extract (10 to 40 mg/kg) for 10 days through gavage. Blood pressures and heart rate were significantly (p < 0.01) reduced in normotensive rats receiving Pq extract (40 mg/kg body weight). Pq extract induced a negative inotropic effect, and endothelium-dependent vasodilation mediated by nitric oxide (NO). Furthermore, preincubation with Pq extract significantly decreased the cytosolic calcium on vascular smooth muscle cells A7r5 in response to L-phenylephrine (PE). Seven metabolites were isolated from the Pq extract, but three flavonoids (10−4 M) showed similar vasodilation to the extract in intact rat aorta as follows: 5,3′,4′-trihydroxy-7-methoxyflavanone (2); 3,5,4′-trihydroxy-7,8,3′-trimethoxyflavone (6); and 5,4′-dihydroxy-3,7,8,3′-tetramethoxyflavone (7). The Pq extract and compounds 2 and 7 significantly (p < 0.05) reduced the contraction to Bay K8644 (10 nM, an agonist of CaV1.2 channels). Administration of Pq decreased cardiac contractility and increased endothelium-dependent and -independent vasodilation.

ACS Style

Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha; Jorge Bórquez; Mario J. Simirgiotis; Ignacio Norambuena; Mario Chiong; Adrián Paredes. Polyphenolic Composition and Hypotensive Effects of Parastrephia quadrangularis (Meyen) Cabrera in Rat. Antioxidants 2019, 8, 591 .

AMA Style

Fredi Cifuentes, Javier Palacios, Chukwuemeka R. Nwokocha, Jorge Bórquez, Mario J. Simirgiotis, Ignacio Norambuena, Mario Chiong, Adrián Paredes. Polyphenolic Composition and Hypotensive Effects of Parastrephia quadrangularis (Meyen) Cabrera in Rat. Antioxidants. 2019; 8 (12):591.

Chicago/Turabian Style

Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha; Jorge Bórquez; Mario J. Simirgiotis; Ignacio Norambuena; Mario Chiong; Adrián Paredes. 2019. "Polyphenolic Composition and Hypotensive Effects of Parastrephia quadrangularis (Meyen) Cabrera in Rat." Antioxidants 8, no. 12: 591.

Journal article
Published: 14 August 2019 in Journal of Basic and Clinical Physiology and Pharmacology
Reads 0
Downloads 0

Background Gongronema latifolium Benth. (family Apocynaceae) leaves (GL) has interesting medicinal properties. The effects of extracts from G. latifolium on blood pressure (BP) and the possible mechanisms of action were also investigated. Methods The ultrahigh resolution liquid chromatography orbitrap MS analysis was used to identify the phytochemicals present. Normotensive Wistar rats were anesthetized with sodium pentobarbitone (40 mg/kg) intraperitoneally, and the jugular vein was cannulated for infusion of drugs while the carotid artery was cannulated for direct BP measurement. GL extract (5–20 mg) alone or with nifedipine (10 mg/kg), atropine (2 mg/kg), L-NAME (5 mg/kg), methyl blue (3 mg/kg) and propranolol (1 mg/kg) were administered intravenously to Wistar rats and direct BP measurements were carried out. Results Systolic and diastolic BP levels (128/90 mm Hg; MAP 103 ± 3 mm Hg) and heart rates were all significantly (p < 0.01) decreased after GL administration. Raised mean arterial pressure (MAP) and heart rate by atropine, L-NAME and methyl blue were significantly (p < 0.01) reduced after GL administration, while propranolol significantly (p < 0.01) inhibited hypotension caused by GL. Infusion of GL reduced MAP (95 ± 3 mm Hg) comparable with nifedipine (93 ± 2 mm Hg), a calcium channel blocker. The phytochemicals identified were 34 compounds, including oleanolic acid derivatives, flavonoids, antioxidant fatty acids, 2 coumarins and 2 iridoids. Conclusions These results suggest that G. latifolium has hypotensive properties mediated by the synergistic activity of the compounds, probably via the β-adrenergic blockade mechanism.

ACS Style

Justin Atiang Beshel; Javier Palacios; Favour Nyoh Beshel; Clement Oshie Nku; Daniel U. Owu; Magdalene Nwokocha; Jorge Bórquez; Mario J. Simirgiotis; Chukwuemeka Nwokocha. Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry. Journal of Basic and Clinical Physiology and Pharmacology 2019, 31, 1 .

AMA Style

Justin Atiang Beshel, Javier Palacios, Favour Nyoh Beshel, Clement Oshie Nku, Daniel U. Owu, Magdalene Nwokocha, Jorge Bórquez, Mario J. Simirgiotis, Chukwuemeka Nwokocha. Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry. Journal of Basic and Clinical Physiology and Pharmacology. 2019; 31 (1):1.

Chicago/Turabian Style

Justin Atiang Beshel; Javier Palacios; Favour Nyoh Beshel; Clement Oshie Nku; Daniel U. Owu; Magdalene Nwokocha; Jorge Bórquez; Mario J. Simirgiotis; Chukwuemeka Nwokocha. 2019. "Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry." Journal of Basic and Clinical Physiology and Pharmacology 31, no. 1: 1.

Journal article
Published: 29 July 2019 in Molecules
Reads 0
Downloads 0

Alkaloids derived from plants have shown great medicinal benefits, and are often reported for their use in cardiovascular disease management. Aristotelia chilensis (Molina) Stuntz (Maqui) has shown important medicinal properties in traditional useage. In this study, we evaluated the effect of the indole-alkaloid aristoteline (ARI), isolated from leaves of Maqui, on vascular reactivity of isolated aortic rings from normotensive rats. ARI induced relaxation (100%) in a concentration-dependent manner in intact or denuded-endothelium aortic rings pre-contracted with phenylephrine (PE; 1 μM). However, a specific soluble guanylyl cyclase inhibitor (ODQ; 1 μM) significantly reduced the relaxation to ARI in aortic rings pre-contracted with PE. In the presence of ARI, the contraction induced by KCl or PE was significantly (p < 0.05) decreased. Interestingly, the potassium channel blockade with 10 μM BaCl2 (Kir), 10 μM glibenclamide (KATP), 1 mM tetraethylammonium (TEA; KCa1.1), or 1 mM 4-aminopyridine (4-AP; Kv) significantly (p < 0.05) reduced the ARI-induced relaxation. ARI significantly (p < 0.05) reduced the contractile response to agonist of CaV1.2 channels (Bay K8644; 10 nM), likely reducing the influx of extracellular calcium through plasma membrane. The mechanisms associated with this process suggest an activation of the potassium channels, a calcium-induced antagonism and endothelium independent vasodilation that possibly involves the nitric oxide-independent soluble guanylate cyclase pathway.

ACS Style

Fernando Romero; Javier Palacios; Ignacio Jofré; Cristian Paz; Chukwuemeka R. Nwokocha; Adrián Paredes; Fredi Cifuentes. Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta. Molecules 2019, 24, 2748 .

AMA Style

Fernando Romero, Javier Palacios, Ignacio Jofré, Cristian Paz, Chukwuemeka R. Nwokocha, Adrián Paredes, Fredi Cifuentes. Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta. Molecules. 2019; 24 (15):2748.

Chicago/Turabian Style

Fernando Romero; Javier Palacios; Ignacio Jofré; Cristian Paz; Chukwuemeka R. Nwokocha; Adrián Paredes; Fredi Cifuentes. 2019. "Aristoteline, an Indole-Alkaloid, Induces Relaxation by Activating Potassium Channels and Blocking Calcium Channels in Isolated Rat Aorta." Molecules 24, no. 15: 2748.

Journal article
Published: 10 January 2019 in Molecules
Reads 0
Downloads 0

Guinep is traditionally used in the management of cardiovascular ailments. This study aims to evaluate its medicinal constituents and effects in the management of myocardial injury in an experimental isoproterenol (ISO) rat model. Sprague-Dawley rats were randomly assigned to four groups: Group 1 was the control group; Group 2 received M. bijugatus extract (100 mg/Kg; MB) for six weeks; Group 3 was given ISO (85 mg/Kg) i.p. twice during a 24-hour period; and Group 4 was given ISO (85 mg/Kg) i.p. and MB extract (100 mg/Kg) for six weeks. The MB was administered orally by gavage, daily. The blood pressure of conscious animals was measured, while ECG was performed under anesthesia. Blood and serum were collected for biochemical and hematological analysis. The ISO group treated with MB showed a significant decrease (p < 0.001) in (SBP), diastolic (DBP), mean arterial (MAP) and heart rate (HR) compared to the ISO only group. Conversely, MB treated rats that were not induced with ISO displayed a significant decreases (p < 0.001) in SBP, DBP, MAP, and HR. ISO significantly elevated the ST segment (p < 0.001) and shortened the QTc interval (p < 0.05), which were recovered after treatment with 100 mg/Kg of MB. In addition, the results showed a significant decrease (p < 0.001) in the heart to body weight ratio of the ISO group treated with MB compared to the ISO only group. Furthermore, the extract normalized the hematological values depressed by the ISO while significantly elevating the platelet count. UHPLC high-resolution orbitrap mass spectrometry analysis results revealed the presence of several antioxidants like vitamin C and related compounds, phenolic acids, flavonoid, fatty acids (oxylipins), and terpene derivatives. The results of this study indicated that Melicoccus bijugatus did display some cardio-protective effects in relation to myocardial injury.

ACS Style

Chukwuemeka R. Nwokocha; Isheba Warren; Javier Palacios; Mario Simirgiotis; Magdalene Nwokocha; Sharon Harrison; Rory Thompson; Adrian Paredes; Jorge Bórquez; Astrid Lavado; Fredi Cifuentes. Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry. Molecules 2019, 24, 235 .

AMA Style

Chukwuemeka R. Nwokocha, Isheba Warren, Javier Palacios, Mario Simirgiotis, Magdalene Nwokocha, Sharon Harrison, Rory Thompson, Adrian Paredes, Jorge Bórquez, Astrid Lavado, Fredi Cifuentes. Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry. Molecules. 2019; 24 (2):235.

Chicago/Turabian Style

Chukwuemeka R. Nwokocha; Isheba Warren; Javier Palacios; Mario Simirgiotis; Magdalene Nwokocha; Sharon Harrison; Rory Thompson; Adrian Paredes; Jorge Bórquez; Astrid Lavado; Fredi Cifuentes. 2019. "Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry." Molecules 24, no. 2: 235.

Journal article
Published: 21 November 2018 in Molecules
Reads 0
Downloads 0

8-Oxo-9-dihydromakomakine is a tetracyclic indole alkaloid extracted from leaves of the Chilean tree Aristotelia chilensis. The present study investigated the effects of this alkaloid on vascular response in tissues isolated from aortic segments obtained from normotensive rats. Our results showed that 8-oxo-9-dihydromakomakine induced a dose-dependent relaxation of aortic rings pre-contracted with phenylephrine (PE; 10−6 M). The vasorelaxation induced by 8-oxo-9-dihydromakomakine in rat aortic rings is independent of endothelium. The pre-incubation of aortic rings with 8-oxo-9-dehydromakomakine (10−4 M) significantly reduced the contractile response to KCl (p < 0.001) more than PE (p < 0.05). The highest dose of 8-oxo-9-dehydromakomakine (10−4 M) drastically reduced the contraction to KCl (6·10−2 M), but after that, PE (10−6 M) caused contraction (p < 0.05) in the same aortic rings. The addition of 8-oxo-9-dihydromakomakine (10−5 M) decreased the contractile response to tetraethylammonium (a voltage-dependent potassium channels blocker; TEA; 5 × 10−3 M; p < 0.01) and BaCl2 (a non-selective inward rectifier potassium channel blocker; 5 × 10−3 M; p < 0.001) in rat aorta. 8-oxo-9-dihydromakomakine (10−5 M) decreased the contractile response to PE in rat aorta in the presence or absence of ouabain (an inhibitor of Na,K-ATPase; 10−3 M; p < 0.05). These results could indicate that 8-oxo-9-dihydromakomakine partially reduces plasma membrane depolarization-induced contraction. In aortic rings depolarized by PE, 8-oxo-9-dihydromakomakine inhibited the contraction induced by the influx of extracellular Ca2+ in a Ca2+ free solution (p < 0.01). 8-oxo-9-dihydromakomakine reduced the contractile response to agonists of voltage-dependent calcium channels type L (Bay K6844; 10−8 M; p < 0.01), likely decreasing the influx of extracellular Ca2+ through the voltage-dependent calcium channels. This study provides the first qualitative analysis indicating that traditional folk medicine Aristotelia chilensis may be protective in the treatment of cardiovascular pathologies.

ACS Style

Fredi Cifuentes; Javier Palacios; Adrián Paredes; Chukwuemeka R. Nwokocha; Cristian Paz. 8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx. Molecules 2018, 23, 3050 .

AMA Style

Fredi Cifuentes, Javier Palacios, Adrián Paredes, Chukwuemeka R. Nwokocha, Cristian Paz. 8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx. Molecules. 2018; 23 (11):3050.

Chicago/Turabian Style

Fredi Cifuentes; Javier Palacios; Adrián Paredes; Chukwuemeka R. Nwokocha; Cristian Paz. 2018. "8-Oxo-9-Dihydromakomakine Isolated from Aristotelia chilensis Induces Vasodilation in Rat Aorta: Role of the Extracellular Calcium Influx." Molecules 23, no. 11: 3050.

Journal article
Published: 26 September 2018 in Phytomedicine
Reads 0
Downloads 0

Xenophyllum poposum is an endemic species of the Andes Cordillera, popularly known as Popusa. Popusa is widely used by mountain communities as a folk medicine to treat altitude sickness and hypertension. The aim of this study is to evaluate the hypotensive effects and vascular reactivity of Popusa extracts and its pure isolated compounds. Hydroalcoholic extract of Xenophyllum poposum (HAE X. poposum; 40 mg/kg dose) were administered to rats by gavage and mean arterial pressures were recorded. Organ bath studies were conducted in endothelium-intact and denuded rings, and the vascular reactivity of the HAE X. poposum extract and its isolated compounds were compared and analysed. Cytosolic Ca2+ was measured in vascular smooth muscle cell line A7r5 using Fura2-AM. HAE X. poposum significantly reduced the mean arterial blood pressure and heart rate in normotensive rats chronically treated with the extract, as well as mice acutely treated with the extract. A negative chronotropic effect was observed in the isolated rat heart. HAE X. poposum induced endothelial vasodilation mediated by nitric oxide (NO), reduced the contractile response to PE, and decreased PE-induced intracellular Ca2+ influx in vascular smooth muscle cells. Pure compounds isolated from HAE X. poposum such as 4-hydroxy-3-(3-methyl-2-butenyl) acetophenone, 5-acetyl-6-hydroxy-2-isopropenyl-2, and 3-dihydrobenzofurane (dihydroeuparin) also triggered endothelium-dependent vasodilation. HAE X. poposum decreases blood pressure, heart rate and vascular response. The vasodilation properties of HAE X. poposum extract and its isolated compounds may act through the endothelial nitric oxide synthase, as well as calcium channel blocker mechanisms. The results of the present study provide the first qualitative analysis that supports the use of X. poposum in traditional folk medicine for the treatment of altitude sickness and hypertension.

ACS Style

Fredi Cifuentes; Javier Palacios; Jovan Kuzmicic; Lorena Carvajal; Fernanda Muñoz; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Ignacio Norambuena-Soto; Mario Chiong; Adrian Paredes. Vasodilator and hypotensive effects of pure compounds and hydroalcoholic extract of Xenophyllum poposum (Phil) V.A Funk (Compositae) on rats. Phytomedicine 2018, 50, 99 -108.

AMA Style

Fredi Cifuentes, Javier Palacios, Jovan Kuzmicic, Lorena Carvajal, Fernanda Muñoz, Cristina Quispe, Chukwuemeka R. Nwokocha, Glauco Morales, Ignacio Norambuena-Soto, Mario Chiong, Adrian Paredes. Vasodilator and hypotensive effects of pure compounds and hydroalcoholic extract of Xenophyllum poposum (Phil) V.A Funk (Compositae) on rats. Phytomedicine. 2018; 50 ():99-108.

Chicago/Turabian Style

Fredi Cifuentes; Javier Palacios; Jovan Kuzmicic; Lorena Carvajal; Fernanda Muñoz; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Ignacio Norambuena-Soto; Mario Chiong; Adrian Paredes. 2018. "Vasodilator and hypotensive effects of pure compounds and hydroalcoholic extract of Xenophyllum poposum (Phil) V.A Funk (Compositae) on rats." Phytomedicine 50, no. : 99-108.

Book chapter
Published: 14 August 2018 in Structure and Chemistry (Part E)
Reads 0
Downloads 0

The vascular tissues are one of the richest sources of NADP(H) oxidase enzymes that are predominantly responsible for the production of oxygen-derived free radicals. NADP(H) oxidase-derived reactive oxygen species (ROS) (as well as other enzyme-derived ROS) play important physiological and pathological roles in the vascular system. Under normal physiological conditions, the ROS species generated are crucial in proinflammatory mechanisms, regulating cell proliferation and maintaining vascular tone. The reactive species produced are kept within rigorously regulated thresholds via either enzymatic or nonenzymatic antioxidant mechanisms. However, various factors (drugs, stress, diseases, etc.) can trigger an imbalance in the redox state in vascular tissues potentiating a wide range of pathologies (hypertension, atherosclerosis, and stroke) directly or indirectly associated with ROS and by extension of oxidative stress. Strong evidence points to NADP(H) oxidase induction, the subsequent high levels of ROS liberated under reduced antioxidant capacity, and their positive correlation in the development of various cardiovascular diseases such as hypertension and atherosclerosis. Mechanisms involved via ROS interacting with endothelial nitric oxide synthase (eNOS), nitric oxide (NO) as well as other enzyme systems (lipooxygenases, xanthine, and cytochrome P450 oxygenases) are necessary for normal vascular function. Pharmacological therapies in the management of diseases with oxidative stress etiology usually target free radicals but not their source of production. This chapter highlights the possible sources of ROS in the vasculature and provides insights on possible targets, evidence-based suggestions for the usefulness of some natural plant products as novel therapeutic agents in the management and approach to mitigate, treat, or manage cardiovascular diseases associated with oxidative stress and profound levels of ROS.

ACS Style

Melissa Reid; Jason Spence; Magdalene Nwokocha; Javier Palacios; Chukwuemeka Nwokocha. The Role of NADP(H) Oxidase Inhibition and Its Implications in Cardiovascular Disease Management Using Natural Plant Products. Structure and Chemistry (Part E) 2018, 58, 43 -59.

AMA Style

Melissa Reid, Jason Spence, Magdalene Nwokocha, Javier Palacios, Chukwuemeka Nwokocha. The Role of NADP(H) Oxidase Inhibition and Its Implications in Cardiovascular Disease Management Using Natural Plant Products. Structure and Chemistry (Part E). 2018; 58 ():43-59.

Chicago/Turabian Style

Melissa Reid; Jason Spence; Magdalene Nwokocha; Javier Palacios; Chukwuemeka Nwokocha. 2018. "The Role of NADP(H) Oxidase Inhibition and Its Implications in Cardiovascular Disease Management Using Natural Plant Products." Structure and Chemistry (Part E) 58, no. : 43-59.

Research article
Published: 26 July 2018 in Oxidative Medicine and Cellular Longevity
Reads 0
Downloads 0

Quinone derivatives like 2-(4-hydroxyphenyl) amino-1,4-naphthoquinone (Q7) are used as antitumor agents usually associated with adverse effects on the cardiovascular system. The objective of this study was to evaluate the cardioprotective effect of ascorbate on Q7-induced cardiovascular response in Wistar rats. In this study, blood pressure, vascular reactivity, and intracellular calcium fluxes were evaluated in cardiomyocytes and the rat aorta. We also measured oxidative stress through lipid peroxidation (TBARS), superoxide dismutase- (SOD-) like activity, and H2O2 generation. Oral treatment of rats with ascorbate (500 mg/kg) for 20 days significantly (p<0.05) reduced the Q7-induced increase (10 mg/kg) in blood pressure and heart rate. The preincubation with ascorbate (2 mM) significantly (p<0.05) attenuated the irregular beating of the atrium induced by Q7 (10−5 M). In addition, ascorbate induced endothelial vasodilation in the presence of Q7 in the intact aortic rings of a rat and reduced the cytosolic calcium levels in vascular smooth muscle cells. Ascorbate also reduced the Q7-induced oxidative stress in vivo. Ascorbate also attenuated Q7-induced SOD-like activity and increased TBARS levels. These results suggest a cardioprotective effect in vivo of ascorbate in animals treated orally with a naphthoquinone derivative by a mechanism involving oxidative stress.

ACS Style

Javier Palacios; José Miguel Fonseca; Fernando Ayavire; Felipe Salas; Mirko Ortiz; Juan Marcelo Sandoval; Julio Benites; Chukwuemeka R. Nwokocha; Ewaldo Zavala; Adrián Paredes; Iván Barría; José Luis Vega; Fredi Cifuentes. Ascorbate Attenuates Oxidative Stress and Increased Blood Pressure Induced by 2-(4-Hydroxyphenyl) Amino-1,4-naphthoquinone in Rats. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -11.

AMA Style

Javier Palacios, José Miguel Fonseca, Fernando Ayavire, Felipe Salas, Mirko Ortiz, Juan Marcelo Sandoval, Julio Benites, Chukwuemeka R. Nwokocha, Ewaldo Zavala, Adrián Paredes, Iván Barría, José Luis Vega, Fredi Cifuentes. Ascorbate Attenuates Oxidative Stress and Increased Blood Pressure Induced by 2-(4-Hydroxyphenyl) Amino-1,4-naphthoquinone in Rats. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-11.

Chicago/Turabian Style

Javier Palacios; José Miguel Fonseca; Fernando Ayavire; Felipe Salas; Mirko Ortiz; Juan Marcelo Sandoval; Julio Benites; Chukwuemeka R. Nwokocha; Ewaldo Zavala; Adrián Paredes; Iván Barría; José Luis Vega; Fredi Cifuentes. 2018. "Ascorbate Attenuates Oxidative Stress and Increased Blood Pressure Induced by 2-(4-Hydroxyphenyl) Amino-1,4-naphthoquinone in Rats." Oxidative Medicine and Cellular Longevity 2018, no. : 1-11.

Journal article
Published: 01 May 2017 in Journal of Ethnopharmacology
Reads 0
Downloads 0

Oral administration of aqueous extract of Artocarpus artilis has cardio-protective functions in myocardial injury, in part, by decreasing the HR, reduced contractility and infarct size. These findings may explain the cardio-protective use of A. altilis in traditional medicine.

ACS Style

Chukwuemeka Nwokocha; Javier Palacios; Mario J. Simirgiotis; Jemesha Thomas; Magdalene Nwokocha; Lauriann Young; Rory Thompson; Fredi Cifuentes; Adrian Paredes; Rupika Delgoda. Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Negative chronotropic and inotropic effects. Journal of Ethnopharmacology 2017, 203, 163 -170.

AMA Style

Chukwuemeka Nwokocha, Javier Palacios, Mario J. Simirgiotis, Jemesha Thomas, Magdalene Nwokocha, Lauriann Young, Rory Thompson, Fredi Cifuentes, Adrian Paredes, Rupika Delgoda. Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Negative chronotropic and inotropic effects. Journal of Ethnopharmacology. 2017; 203 ():163-170.

Chicago/Turabian Style

Chukwuemeka Nwokocha; Javier Palacios; Mario J. Simirgiotis; Jemesha Thomas; Magdalene Nwokocha; Lauriann Young; Rory Thompson; Fredi Cifuentes; Adrian Paredes; Rupika Delgoda. 2017. "Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: Negative chronotropic and inotropic effects." Journal of Ethnopharmacology 203, no. : 163-170.

Journal article
Published: 01 November 2016 in Journal of Ethnopharmacology
Reads 0
Downloads 0

HAE S. nutans and its isolated compounds caused vasodilation in rat aorta in absence of endothelium, suggesting its vasodilator properties is endothelium-dependent (NO) and or independent, and may involve a modulation of the calcium channels. This result is of clinical interest as potential therapy control of blood pressure.

ACS Style

Adrian Paredes; Javier Palacios; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Jovan Kuzmicic; Fredi Cifuentes. Hydroalcoholic extract and pure compounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms. Journal of Ethnopharmacology 2016, 192, 99 -107.

AMA Style

Adrian Paredes, Javier Palacios, Cristina Quispe, Chukwuemeka R. Nwokocha, Glauco Morales, Jovan Kuzmicic, Fredi Cifuentes. Hydroalcoholic extract and pure compounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms. Journal of Ethnopharmacology. 2016; 192 ():99-107.

Chicago/Turabian Style

Adrian Paredes; Javier Palacios; Cristina Quispe; Chukwuemeka R. Nwokocha; Glauco Morales; Jovan Kuzmicic; Fredi Cifuentes. 2016. "Hydroalcoholic extract and pure compounds from Senecio nutans Sch. Bip (Compositae) induce vasodilation in rat aorta through endothelium-dependent and independent mechanisms." Journal of Ethnopharmacology 192, no. : 99-107.

Research article
Published: 08 September 2016 in Oxidative Medicine and Cellular Longevity
Reads 0
Downloads 0

The vascular endothelium plays an essential role in the control of the blood flow. Pharmacological agents like quinone (menadione) at various doses modulate this process in a variety of ways. In this study,Q7, a 2-phenylamino-1,4-naphthoquinone derivative, significantly increased oxidative stress and induced vascular dysfunction at concentrations that were not cytotoxic to endothelial or vascular smooth muscle cells.Q7reduced nitric oxide (NO) levels and endothelial vasodilation to acetylcholine in rat aorta. It also blunted the calcium release from intracellular stores by increasing the phenylephrine-induced vasoconstriction when CaCl2was added to a calcium-free medium but did not affect the influx of calcium from extracellular space.Q7increased the vasoconstriction to BaCl2(10−3 M), an inward rectifying K+channels blocker, and blocked the vasodilation to KCl (10−2 M) in aortic rings precontracted with BaCl2. This was recovered with sodium nitroprusside (10−8 M), a NO donor. In conclusion,Q7induced vasoconstriction was through a modulation of cellular mechanisms involving calcium fluxes through K+channels, and oxidative stress induced endothelium damage. These findings contribute to the characterization of new quinone derivatives with low cytotoxicity able to pharmacologically modulate vasodilation.

ACS Style

Javier Palacios; Fredi Cifuentes; Jaime A. Valderrama; Julio Benites; David Ríos; Constanza González; Mario Chiong; Benjam&#xed Cartes-Saavedra; Carlos Lafourcade; Ursula Wyneken; Pamela González; Gareth I. Owen; Fabián Pardo; Luis Sobrevia; Pedro Buc Calderon. Modulatory Effect of 2-(4-Hydroxyphenyl)amino-1,4-naphthoquinone on Endothelial Vasodilation in Rat Aorta. Oxidative Medicine and Cellular Longevity 2016, 2016, 1 -12.

AMA Style

Javier Palacios, Fredi Cifuentes, Jaime A. Valderrama, Julio Benites, David Ríos, Constanza González, Mario Chiong, Benjam&#xed Cartes-Saavedra, Carlos Lafourcade, Ursula Wyneken, Pamela González, Gareth I. Owen, Fabián Pardo, Luis Sobrevia, Pedro Buc Calderon. Modulatory Effect of 2-(4-Hydroxyphenyl)amino-1,4-naphthoquinone on Endothelial Vasodilation in Rat Aorta. Oxidative Medicine and Cellular Longevity. 2016; 2016 ():1-12.

Chicago/Turabian Style

Javier Palacios; Fredi Cifuentes; Jaime A. Valderrama; Julio Benites; David Ríos; Constanza González; Mario Chiong; Benjam&#xed Cartes-Saavedra; Carlos Lafourcade; Ursula Wyneken; Pamela González; Gareth I. Owen; Fabián Pardo; Luis Sobrevia; Pedro Buc Calderon. 2016. "Modulatory Effect of 2-(4-Hydroxyphenyl)amino-1,4-naphthoquinone on Endothelial Vasodilation in Rat Aorta." Oxidative Medicine and Cellular Longevity 2016, no. : 1-12.

Journal article
Published: 01 February 2016 in Journal of Ethnopharmacology
Reads 0
Downloads 0
ACS Style

Fredi Cifuentes; Adrián Paredes; Javier Palacios; Fernanda Muñoz; Lorena Carvajal; Chukwuemeka R. Nwokocha; Glauco Morales. Hypotensive and antihypertensive effects of a hydroalcoholic extract from Senecio nutans Sch. Bip. (Compositae) in mice: Chronotropic and negative inotropic effect, a nifedipine-like action. Journal of Ethnopharmacology 2016, 179, 367 -374.

AMA Style

Fredi Cifuentes, Adrián Paredes, Javier Palacios, Fernanda Muñoz, Lorena Carvajal, Chukwuemeka R. Nwokocha, Glauco Morales. Hypotensive and antihypertensive effects of a hydroalcoholic extract from Senecio nutans Sch. Bip. (Compositae) in mice: Chronotropic and negative inotropic effect, a nifedipine-like action. Journal of Ethnopharmacology. 2016; 179 ():367-374.

Chicago/Turabian Style

Fredi Cifuentes; Adrián Paredes; Javier Palacios; Fernanda Muñoz; Lorena Carvajal; Chukwuemeka R. Nwokocha; Glauco Morales. 2016. "Hypotensive and antihypertensive effects of a hydroalcoholic extract from Senecio nutans Sch. Bip. (Compositae) in mice: Chronotropic and negative inotropic effect, a nifedipine-like action." Journal of Ethnopharmacology 179, no. : 367-374.

Journal article
Published: 05 October 2015 in Cardiovascular Toxicology
Reads 0
Downloads 0
ACS Style

Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha; Fabian Pardo; Luis Sobrevia. Erratum to: Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide. Cardiovascular Toxicology 2015, 16, 89 -89.

AMA Style

Fredi Cifuentes, Javier Palacios, Chukwuemeka R. Nwokocha, Fabian Pardo, Luis Sobrevia. Erratum to: Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide. Cardiovascular Toxicology. 2015; 16 (1):89-89.

Chicago/Turabian Style

Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha; Fabian Pardo; Luis Sobrevia. 2015. "Erratum to: Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide." Cardiovascular Toxicology 16, no. 1: 89-89.

Journal article
Published: 10 February 2015 in Cardiovascular Toxicology
Reads 0
Downloads 0

Arsenic trioxide (As2O3) is used clinically in the management of acute promyelocytic leukemia, and the use of electrocardiogram (ECG) in this management is important as arsenic use may cause distortion of the electrical properties with its attendant sequel. We studied the effect of As2O3 on vasomotion in rat aortic rings using isometric tension recordings and ECG in anesthetized rats. The results showed that As2O3 (10−5 M) significantly (p −5 M ACh)- and KCl (10 mM)-induced vasomotion, and it also increased the relaxation time (R t) of vasomotion. This effect was restored by 10−8 M sodium nitroprusside (nitric oxide donor). ACh-induced NO release in the aorta was blunted in the presence of As2O3. The corrected QT interval (QTc) of the ECG, and time dilation (T d) of the pulse wave in the tail artery of the anesthetized rat were significantly (p

ACS Style

Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha. Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide. Cardiovascular Toxicology 2015, 16, 79 -88.

AMA Style

Fredi Cifuentes, Javier Palacios, Chukwuemeka R. Nwokocha. Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide. Cardiovascular Toxicology. 2015; 16 (1):79-88.

Chicago/Turabian Style

Fredi Cifuentes; Javier Palacios; Chukwuemeka R. Nwokocha. 2015. "Synchronization in the Heart Rate and the Vasomotion in Rat Aorta: Effect of Arsenic Trioxide." Cardiovascular Toxicology 16, no. 1: 79-88.