Quantitative MRI methods that estimate multiple physical parameters simultaneously often require the fitting of a computational complex signal model defined through the Bloch equations. Repeated Bloch simulations can be avoided by matching the measured signal with a precomputed signal dictionary on a discrete parameter grid (i.e. lookup table) as used in MR Fingerprinting. However, accurate estimation requires discretizing each parameter with a high resolution and consequently high computational and memory costs for dictionary generation, storage, and matching. Here, we reduce the required parameter resolution by approximating the signal between grid points through B-spline interpolation. The interpolant and its gradient are evaluated efficiently which enables a least-squares fitting method for parameter mapping. The resolution of each parameter was minimized while obtaining a user-specified interpolation accuracy. The method was evaluated by phantom and in-vivo experiments using fully-sampled and undersampled unbalanced (FISP) MR fingerprinting acquisitions. Bloch simulations incorporated relaxation effects (T1,T2), proton density (PD), receiver phase (φ0), transmit field inhomogeneity (B1+), and slice profile. Parameter maps were compared with those obtained from dictionary matching, where the parameter resolution was.
Precision greenhouse agriculture enhances plant health and crop yields by continuously monitoring key plant parameters. Stem diameter is such a parameter and is monitored to support decisions on plant care. However, traditional contact-based methods induce thigmomorphogenic effects that impact plant growth. Here, we introduce the Optical Caliper (OC), a novel contactless device for precise, non-invasive stem diameter measurement. The OC operates by projecting a collimated light beam to cast a shadow of the stem onto a high-resolution image sensor. The shadow size is a measure for the stem diameter. Controlled laboratory tests show the OC offers an accuracy comparable to that of a Digital Caliper (DC). Field trials on irregular tomato and cucumber stems demonstrate a repeatability of 0.1–0.2 mm. The OC’s non-invasive design and high repeatability exceed the performance of a DC, making it particularly suited for accurately monitoring soft, variable plant structures. Bringing the advantage of avoiding thigmomophogenic effects and thus optimizing crop yield, the OC is a promising tool for high-throughput plant phenotyping and precision agriculture applications.