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Dr. Silvia Arribas
Department of Physiology, Faculty of Medicine, Universidad Autonoma de Madrid, C/ Arzobispo Morcillo 2 28029-Madrid, Spain

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0 Confocal Microscopy
0 Hypertension
0 Nutrition
0 Fetal Programming
0 Oxidative Stress Biomarkers

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Hypertension
Confocal Microscopy
Fetal Programming
Nutrition
Oxidative Stress Biomarkers

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Journal article
Published: 12 August 2021 in International Journal of Environmental Research and Public Health
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Adolescent pregnancy remains a health issue worldwide also in developed countries, since it has been associated with adverse maternal and neonatal outcomes. Some data suggest that very young adolescents have higher risk, likely due to immaturity. Therefore, we aimed to assess the influence of maternal age on complications during gestation and labor in pregnant women between 13 and 19 years of age. In particular, we evaluated the possible association between maternal age and obstetric, fetal and labor complications. This is a retrospective, observational and exploratory study conducted at Hospital Universitario La Paz (HULP, Madrid, Spain). The clinical history of 279 women who delivered between 2013 and 2018 was analyzed. Maternal age and the presence of maternal, fetal and labor complications, as well as risk of postpartum depression and breastfeeding intention, were recorded. General regression models were used to analyze the contribution of maternal age on each complication. The percentage of adolescent pregnancies at HULP between 2013 and 2018 was 0.9%. The risk of all the maternal complications analyzed decreased significantly with every year of age of the mother (hyperemesis, lower back pain, anemia, gestational diabetes mellitus, and threat of premature labor and premature rupture of membranes). Every year of maternal age decreased 0.8-fold [0.8; 0.9] the prevalence of fetal complications and also reduced the risk of C-section, postpartum hemorrhage and obstetrical hysterectomy. Furthermore, higher maternal age increased 1.1-fold [1.0; 1.2] the breastfeeding intention. In conclusion, young adolescents are at higher risk of complications during pregnancy and labor.

ACS Style

Maria de la Calle; Jose L. Bartha; Cristina M. Lopez; Miriam Turiel; Nuria Martinez; Silvia M. Arribas; David Ramiro-Cortijo. Younger Age in Adolescent Pregnancies Is Associated with Higher Risk of Adverse Outcomes. International Journal of Environmental Research and Public Health 2021, 18, 8514 .

AMA Style

Maria de la Calle, Jose L. Bartha, Cristina M. Lopez, Miriam Turiel, Nuria Martinez, Silvia M. Arribas, David Ramiro-Cortijo. Younger Age in Adolescent Pregnancies Is Associated with Higher Risk of Adverse Outcomes. International Journal of Environmental Research and Public Health. 2021; 18 (16):8514.

Chicago/Turabian Style

Maria de la Calle; Jose L. Bartha; Cristina M. Lopez; Miriam Turiel; Nuria Martinez; Silvia M. Arribas; David Ramiro-Cortijo. 2021. "Younger Age in Adolescent Pregnancies Is Associated with Higher Risk of Adverse Outcomes." International Journal of Environmental Research and Public Health 18, no. 16: 8514.

Journal article
Published: 11 July 2021 in Nutrients
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There are numerous dietary recommendations during pregnancy. However, there are limited recommendations during the lactation period, a nutritionally vulnerable period for women. The Mediterranean Diet and adherence to the Healthy Food Pyramid (HFP) is considered as the standard for healthy eating. In this study, we investigated the differences in adherence to the HFP in pregnant, lactating, and non-pregnant/non-lactating (NPNL) women concerning sociodemographic factors. A sociodemographic and nutritional and lifestyle questionnaire (AP-Q) were used to assess adherence to the HFP, including lifestyle. The AP-Q score ranges from 0 to 10 meaning the higher the score, the greater the adherence to the HFP. Lactating women had the lowest AP-Q score (6.13 [5.31; 6.82]) compared to the pregnant (6.39 [5.56; 7.05]) and NPNL women (6.27 [5.43; 6.88]), while pregnant women had the highest scores. Maternal age was positively correlated with AP-Q score in pregnant (rho = 0.22; p-Value < 0.001) and lactating women (rho = 0.18; p-Value < 0.001), but not in NPNL women. Educational level and monthly income had a positive influence on the degree of adherence to the HFP. In conclusion, breastfeeding mothers of young age and low socioeconomic and educational level would be the target population to carry out nutritional interventions that improve their adherence to the HFP. The knowledge gained from this study can help to design recommendation guidelines and nutritional educational interventions for a given population.

ACS Style

Andrea Gila-Díaz; Ariadna Witte Castro; Gloria Herranz Carrillo; Pratibha Singh; William Yakah; Silvia Arribas; David Ramiro-Cortijo. Assessment of Adherence to the Healthy Food Pyramid in Pregnant and Lactating Women. Nutrients 2021, 13, 2372 .

AMA Style

Andrea Gila-Díaz, Ariadna Witte Castro, Gloria Herranz Carrillo, Pratibha Singh, William Yakah, Silvia Arribas, David Ramiro-Cortijo. Assessment of Adherence to the Healthy Food Pyramid in Pregnant and Lactating Women. Nutrients. 2021; 13 (7):2372.

Chicago/Turabian Style

Andrea Gila-Díaz; Ariadna Witte Castro; Gloria Herranz Carrillo; Pratibha Singh; William Yakah; Silvia Arribas; David Ramiro-Cortijo. 2021. "Assessment of Adherence to the Healthy Food Pyramid in Pregnant and Lactating Women." Nutrients 13, no. 7: 2372.

Journal article
Published: 05 June 2021 in Pathophysiology
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Fetal undernutrition is a risk factor for cardiovascular diseases. Male offspring from rats exposed to undernutrition during gestation (MUN) exhibit oxidative stress during perinatal life and develop cardiac dysfunction in ageing. Angiotensin-II is implicated in oxidative stress-mediated cardiovascular fibrosis and remodeling, and lactation is a key developmental window. We aimed to assess if alterations in RAS during lactation participate in cardiac dysfunction associated with fetal undernutrition. Control dams received food ad libitum, and MUN had 50% nutrient restriction during the second half of gestation. Both dams were fed ad libitum during lactation, and male offspring were studied at weaning. We assessed: ventricular structure and function (echocardiography); blood pressure (intra-arterially, anesthetized rats); collagen content and intramyocardial artery structure (Sirius red, Masson Trichromic); myocardial and intramyocardial artery RAS receptors (immunohistochemistry); plasma angiotensin-II (ELISA) and TGF-β1 protein expression (Western Blot). Compared to Control, MUN offspring exhibited significantly higher plasma Angiotensin-II and a larger left ventricular mass, as well as larger intramyocardial artery media/lumen, interstitial collagen and perivascular collagen. In MUN hearts, TGF-β1 tended to be higher, and the end-diastolic diameter and E/A ratio were significantly lower with no differences in ejection fraction or blood pressure. In the myocardium, no differences between groups were detected in AT1, AT2 or Mas receptors, with MrgD being significantly lower in the MUN group. In intramyocardial arteries from MUN rats, AT1 and Mas receptors were significantly elevated, while AT2 and MrgD were lower compared to Control. Conclusions. In rats exposed to fetal undernutrition, RAS disbalance and associated cardiac remodeling during lactation may set the basis for later heart dysfunction.

ACS Style

Pilar Rodríguez-Rodríguez; Maria Vieira-Rocha; Begoña Quintana-Villamandos; Ignacio Monedero-Cobeta; Parichat Prachaney; Angel López de Pablo; Maria González; Manuela Morato; Carmen Diniz; Silvia Arribas. Implication of RAS in Postnatal Cardiac Remodeling, Fibrosis and Dysfunction Induced by Fetal Undernutrition. Pathophysiology 2021, 28, 273 -290.

AMA Style

Pilar Rodríguez-Rodríguez, Maria Vieira-Rocha, Begoña Quintana-Villamandos, Ignacio Monedero-Cobeta, Parichat Prachaney, Angel López de Pablo, Maria González, Manuela Morato, Carmen Diniz, Silvia Arribas. Implication of RAS in Postnatal Cardiac Remodeling, Fibrosis and Dysfunction Induced by Fetal Undernutrition. Pathophysiology. 2021; 28 (2):273-290.

Chicago/Turabian Style

Pilar Rodríguez-Rodríguez; Maria Vieira-Rocha; Begoña Quintana-Villamandos; Ignacio Monedero-Cobeta; Parichat Prachaney; Angel López de Pablo; Maria González; Manuela Morato; Carmen Diniz; Silvia Arribas. 2021. "Implication of RAS in Postnatal Cardiac Remodeling, Fibrosis and Dysfunction Induced by Fetal Undernutrition." Pathophysiology 28, no. 2: 273-290.

Journal article
Published: 22 May 2021 in Nutrients
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The first month of lactation is a vulnerable nutritional period for the mother. Our aims were (1) to evaluate the nutritional status of breastfeeding women in the first month of lactation, and (2) to explore different aspects of nutrition and lifestyle through a multidimensional approach. A total of 30 healthy breastfeeding women were enrolled in this study. Dietary pattern was assessed through a 72-hour dietary recall questionnaire (days 7 and 28 postpartum) and data were compared with Dietary Recommendation Values (DRV), and through the Adherence to Healthy Food Pyramid (HFP) questionnaire (day 28). Anthropometric parameters were evaluated by bioimpedance. Using factor analysis, nutritional dimensions were extracted, and linear regression models were used to analyze the association between anthropometric parameters and dimensions. Compared to the DRV, women showed insufficient energy, water, vitamin D, and potassium intake and excessive proteins, vitamins B1, B2, B3, B6, B12, and iron intake. We observed a moderate adherence to the HFP, with women being high in the fruits, vegetables, and oil categories, and low adherence to the physical activity, dairy products, and hydration categories. The nutritional dimension, including the HFP categories of physical activity, hydration, and animal protein intake together, was negatively associated with body weight (β = −3.7 ± 1.7; p-value = 0.047). In conclusion, during the first month postpartum, breastfeeding women exhibited several nutritional imbalances and poor physical activity negatively influencing anthropometric parameters. We propose a multidimensional approach to assess the nutritional status of breastfeeding women as a tool to detect specific deficiencies, allowing for personalized counseling.

ACS Style

Andrea Gila-Díaz; Nuria Díaz-Rullo Alcántara; Gloria Herranz Carrillo; Pratibha Singh; Silvia Arribas; David Ramiro-Cortijo. Multidimensional Approach to Assess Nutrition and Lifestyle in Breastfeeding Women during the First Month of Lactation. Nutrients 2021, 13, 1766 .

AMA Style

Andrea Gila-Díaz, Nuria Díaz-Rullo Alcántara, Gloria Herranz Carrillo, Pratibha Singh, Silvia Arribas, David Ramiro-Cortijo. Multidimensional Approach to Assess Nutrition and Lifestyle in Breastfeeding Women during the First Month of Lactation. Nutrients. 2021; 13 (6):1766.

Chicago/Turabian Style

Andrea Gila-Díaz; Nuria Díaz-Rullo Alcántara; Gloria Herranz Carrillo; Pratibha Singh; Silvia Arribas; David Ramiro-Cortijo. 2021. "Multidimensional Approach to Assess Nutrition and Lifestyle in Breastfeeding Women during the First Month of Lactation." Nutrients 13, no. 6: 1766.

Journal article
Published: 05 March 2021 in Journal of Personalized Medicine
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Early detection of gestational complications is a priority in obstetrics. In our social context, this is linked to maternity age. Most studies are focused on biological factors. However, pregnancy is also influenced by social and psychological factors, which have not been deeply explored. We aimed to identify biopsychosocial risk and protective factors associated with the development of maternal and fetal complications. We enrolled 182 healthy pregnant women, and plasma melatonin and cortisol levels were measured in the first trimester by chemiluminescent immunoassays. At different time points along gestation, women answered several questionnaires (positive and negative affect schedule, hospital anxiety and depression scale, pregnancy concerns scale, life orientation test, resilience scale, life satisfaction scale and life–work conflicts scale). They were followed up until delivery and categorized as normal pregnancy, maternal or fetal complications. Maternal complications were associated with low melatonin (OR = 0.99 [0.98; 1.00]; p-value = 0.08) and life satisfaction (OR = 0.64 [0.41; 0.93]; p-value = 0.03) and fetal complications were associated with high cortisol (OR = 1.06 [1.02; 1.13]; p-value = 0.04), anxiety (OR = 2.21 [1.10; 4.55]; p-value = 0.03) and life–work conflicts (OR = 1.92 [1.04; 3.75]; p-value = 0.05). We conclude that psychological factors influence pregnancy outcomes in association with melatonin and cortisol alterations. High maternal melatonin and life satisfaction levels could be potential protective factors against the development of maternal complications during pregnancy. Low anxiety and cortisol levels and reduced work–life conflicts could prevent fetal complications.

ACS Style

David Ramiro-Cortijo; Maria de la Calle; Vanesa Benitez; Andrea Gila-Diaz; Bernardo Moreno-Jiménez; Silvia Arribas; Eva Garrosa. Maternal Psychological and Biological Factors Associated to Gestational Complications. Journal of Personalized Medicine 2021, 11, 183 .

AMA Style

David Ramiro-Cortijo, Maria de la Calle, Vanesa Benitez, Andrea Gila-Diaz, Bernardo Moreno-Jiménez, Silvia Arribas, Eva Garrosa. Maternal Psychological and Biological Factors Associated to Gestational Complications. Journal of Personalized Medicine. 2021; 11 (3):183.

Chicago/Turabian Style

David Ramiro-Cortijo; Maria de la Calle; Vanesa Benitez; Andrea Gila-Diaz; Bernardo Moreno-Jiménez; Silvia Arribas; Eva Garrosa. 2021. "Maternal Psychological and Biological Factors Associated to Gestational Complications." Journal of Personalized Medicine 11, no. 3: 183.

Journal article
Published: 10 February 2021 in Journal of Clinical Medicine
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Cognitive maternal adaptation during pregnancy may influence biological variables, maternal psychological, and neonatal health. We hypothesized that pregnant women with numerous general resources and less negative emotions would have a better coping with a positive influence on neonatal birth weight and maternal psychological health. The study included 131 healthy pregnant women. A blood sample was obtained in the first trimester to assess biological variables (polyphenols, hematological and biochemical parameters). Psychological variables (negative affect, anxiety, optimism, resilience, family–work conflicts, pregnancy concerns, general resources, and life satisfaction) were evaluated at several time points along gestation, and birth weight was recorded. Hierarchical linear regression models were used to associate the above parameters with maternal psychological outcome at the end of gestation (depression, resilience, and optimism) and neonatal outcome (birth weight). Maternal depression was associated with leukocytes (β = 0.08 ± 0.03, p-value = 0.003), cholesterol (β = 0.01 ± 0.002, p-value = 0.026), and pregnancy concerns (β = 0.31 ± 0.09, p-value = 0.001). Maternal resilience was associated with leukocytes (β = −0.14 ± 0.09, p-value = 0.010) and life satisfaction (β = 0.82 ± 0.08, p-value = 0.001), and maternal optimism was associated with polyphenol levels (β = 0.002 ± 0.001, p-value = 0.018) and life satisfaction (β = 0.49 ± 0.04, p-value = 0.001). Birth weight was associated with maternal resilience (β = 370.2 ± 97.0, p-value = 0.001), red blood cells (β = 480.3 ± 144.4, p-value = 0.001), and life satisfaction (β = 423.3 ± 32.6, p-value = 0.001). We found associations between maternal psychological, blood variables, and birth weight and maternal depression. This study reveals the relevance of psychological health during pregnancy for maternal and neonatal outcome, and it emphasizes the need to consider it in preventive policies in the obstetric field.

ACS Style

David Ramiro-Cortijo; María de la Calle; Andrea Gila-Díaz; Bernardo Moreno-Jiménez; Maria Martin-Cabrejas; Silvia Arribas; Eva Garrosa. Maternal Resources, Pregnancy Concerns, and Biological Factors Associated to Birth Weight and Psychological Health. Journal of Clinical Medicine 2021, 10, 695 .

AMA Style

David Ramiro-Cortijo, María de la Calle, Andrea Gila-Díaz, Bernardo Moreno-Jiménez, Maria Martin-Cabrejas, Silvia Arribas, Eva Garrosa. Maternal Resources, Pregnancy Concerns, and Biological Factors Associated to Birth Weight and Psychological Health. Journal of Clinical Medicine. 2021; 10 (4):695.

Chicago/Turabian Style

David Ramiro-Cortijo; María de la Calle; Andrea Gila-Díaz; Bernardo Moreno-Jiménez; Maria Martin-Cabrejas; Silvia Arribas; Eva Garrosa. 2021. "Maternal Resources, Pregnancy Concerns, and Biological Factors Associated to Birth Weight and Psychological Health." Journal of Clinical Medicine 10, no. 4: 695.

Journal article
Published: 28 December 2020 in International Journal of Molecular Sciences
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Fetal undernutrition programs cardiometabolic diseases, with higher susceptibility in males. The mechanisms implicated are not fully understood and may be related to sex differences in placental adaptation. To evaluate this hypothesis, we investigated placental oxidative balance, vascularization, glucocorticoid barrier, and fetal growth in rats exposed to 50% global nutrient restriction from gestation day 11 (MUN, n = 8) and controls (n = 8). At gestation day 20 (G20), we analyzed maternal, placental, and fetal weights; oxidative damage, antioxidants, corticosterone, and PlGF (placental growth factor, spectrophotometry); and VEGF (vascular endothelial growth factor), 11β-HSD2, p22phox, XO, SOD1, SOD2, SOD3, catalase, and UCP2 expression (Western blot). Compared with controls, MUN dams exhibited lower weight and plasma proteins and higher corticosterone and catalase without oxidative damage. Control male fetuses were larger than female fetuses. MUN males had higher plasma corticosterone and were smaller than control males, but had similar weight than MUN females. MUN male placenta showed higher XO and lower 11β-HSD2, VEGF, SOD2, catalase, UCP2, and feto-placental ratio than controls. MUN females had similar feto-placental ratio and plasma corticosterone than controls. Female placenta expressed lower XO, 11β-HSD2, and SOD3; similar VEGF, SOD1, SOD2, and UCP2; and higher catalase than controls, being 11β-HSD2 and VEGF higher compared to MUN males. Male placenta has worse adaptation to undernutrition with lower efficiency, associated with oxidative disbalance and reduced vascularization and glucocorticoid barrier. Glucocorticoids and low nutrients may both contribute to programming in MUN males.

ACS Style

Sophida Phuthong; Cynthia Guadalupe Reyes-Hernández; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Raquel González-Blázquez; M. Carmen González; Angel Luis López De Pablo; Silvia M. Arribas. Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition. International Journal of Molecular Sciences 2020, 22, 237 .

AMA Style

Sophida Phuthong, Cynthia Guadalupe Reyes-Hernández, Pilar Rodríguez-Rodríguez, David Ramiro-Cortijo, Marta Gil-Ortega, Raquel González-Blázquez, M. Carmen González, Angel Luis López De Pablo, Silvia M. Arribas. Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition. International Journal of Molecular Sciences. 2020; 22 (1):237.

Chicago/Turabian Style

Sophida Phuthong; Cynthia Guadalupe Reyes-Hernández; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Raquel González-Blázquez; M. Carmen González; Angel Luis López De Pablo; Silvia M. Arribas. 2020. "Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition." International Journal of Molecular Sciences 22, no. 1: 237.

Proceedings
Published: 30 November 2020 in Medical Sciences Forum
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The cocoa shell is a by-product generated by the cocoa processing industry that could be used as a nutraceutical owing to the significant amounts of bioactive compounds it contains. This work aimed to study the bioaccessibility of phenolic compounds present in the flour (CSF) and an aqueous extract (CSE) from cocoa shells through an in vitro simulated digestion and to assess their antioxidant capacity in vitro by using intestinal and hepatic cell culture models (IEC-6 and HepG2 cells). The bioaccessibility of phenolic compounds was determined using a simulated in vitro digestion model (INFOGEST). Total phenolic compounds (TPC) and antioxidant activity were measured using in vitro techniques. Reactive oxygen species (ROS) were evaluated in IEC-6 and HepG2 cells after t-BOOH stimulation. TPC present in CSE were more bioaccessible than phenolic compounds present in CSF. During digestion, the bioaccessibility of phenolic compounds from CSF fluctuated in the gastric (2.8 mg/g), intestinal (7.6 mg/g), and colonic (5.7 mg/g) phases. Similarly, for the phenolics of CSE, the bioaccessibility increased from 50.6 mg/g in the gastric phase to 53.4 mg/g in the intestinal phase and decreased in the colonic phase to 37.2 mg/g. The in vitro antioxidant capacity followed a similar behavior, increasing throughout the digestion in CSF (8.8- to 10.6-fold) and CSE (6.0- to 7.4-fold). Digested CSF and CSE were not cytotoxic for IEC-6 and HepG2 cells and protected their viability under oxidative stress conditions (93–100%). t-BOOH-induced ROS were prevented by CSF (72–88%) and CSE (81–94%) bioaccessible fractions in both intestinal and hepatic cells. In conclusion, cocoa shells are a source of potentially bioavailable antioxidant phenolic compounds that may protect cells from oxidative stress.

ACS Style

Silvia Cañas; Miguel Rebollo-Hernanz; Yolanda Aguilera; Vanesa Benítez; Cheyenne Braojos; Silvia Arribas; María Martín-Cabrejas. Bioaccessibility of Phenolic Compounds from Cocoa Shell Subjected to In Vitro Digestion and Its Antioxidant Activity in Intestinal and Hepatic Cells. Medical Sciences Forum 2020, 2, 5 .

AMA Style

Silvia Cañas, Miguel Rebollo-Hernanz, Yolanda Aguilera, Vanesa Benítez, Cheyenne Braojos, Silvia Arribas, María Martín-Cabrejas. Bioaccessibility of Phenolic Compounds from Cocoa Shell Subjected to In Vitro Digestion and Its Antioxidant Activity in Intestinal and Hepatic Cells. Medical Sciences Forum. 2020; 2 (1):5.

Chicago/Turabian Style

Silvia Cañas; Miguel Rebollo-Hernanz; Yolanda Aguilera; Vanesa Benítez; Cheyenne Braojos; Silvia Arribas; María Martín-Cabrejas. 2020. "Bioaccessibility of Phenolic Compounds from Cocoa Shell Subjected to In Vitro Digestion and Its Antioxidant Activity in Intestinal and Hepatic Cells." Medical Sciences Forum 2, no. 1: 5.

Journal article
Published: 13 November 2020 in International Journal of Molecular Sciences
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One of the consequences of high altitude (hypobaric hypoxia) exposure is the development of right ventricular hypertrophy (RVH). One particular type of exposure is long-term chronic intermittent hypobaric hypoxia (CIH); the molecular alterations in RVH in this particular condition are less known. Studies show an important role of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex-induced oxidative stress and protein kinase activation in different models of cardiac hypertrophy. The aim was to determine the oxidative level, NADPH oxidase expression and MAPK activation in rats with RVH induced by CIH. Male Wistar rats were randomly subjected to CIH (2 days hypoxia/2 days normoxia; n = 10) and normoxia (NX; n = 10) for 30 days. Hypoxia was simulated with a hypobaric chamber. Measurements in the RV included the following: hypertrophy, Nox2, Nox4, p22phox, LOX-1 and HIF-1α expression, lipid peroxidation and H2O2 concentration, and p38α and Akt activation. All CIH rats developed RVH and showed an upregulation of LOX-1, Nox2 and p22phox and an increase in lipid peroxidation, HIF-1α stabilization and p38α activation. Rats with long-term CIH-induced RVH clearly showed Nox2, p22phox and LOX-1 upregulation and increased lipid peroxidation, HIF-1α stabilization and p38α activation. Therefore, these molecules may be considered new targets in CIH-induced RVH.

ACS Style

Eduardo Pena; Patricia Siques; Julio Brito; Silvia M. Arribas; Rainer Böger; Juliane Hannemann; Fabiola León-Velarde; M. Carmen González; M. Rosario López; Ángel Luis López De Pablo. Nox2 Upregulation and p38α MAPK Activation in Right Ventricular Hypertrophy of Rats Exposed to Long-Term Chronic Intermittent Hypobaric Hypoxia. International Journal of Molecular Sciences 2020, 21, 8576 .

AMA Style

Eduardo Pena, Patricia Siques, Julio Brito, Silvia M. Arribas, Rainer Böger, Juliane Hannemann, Fabiola León-Velarde, M. Carmen González, M. Rosario López, Ángel Luis López De Pablo. Nox2 Upregulation and p38α MAPK Activation in Right Ventricular Hypertrophy of Rats Exposed to Long-Term Chronic Intermittent Hypobaric Hypoxia. International Journal of Molecular Sciences. 2020; 21 (22):8576.

Chicago/Turabian Style

Eduardo Pena; Patricia Siques; Julio Brito; Silvia M. Arribas; Rainer Böger; Juliane Hannemann; Fabiola León-Velarde; M. Carmen González; M. Rosario López; Ángel Luis López De Pablo. 2020. "Nox2 Upregulation and p38α MAPK Activation in Right Ventricular Hypertrophy of Rats Exposed to Long-Term Chronic Intermittent Hypobaric Hypoxia." International Journal of Molecular Sciences 21, no. 22: 8576.

Proceedings
Published: 09 November 2020 in Proceedings
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Coffee pulp is a by-product generated during coffee processing, producing environmental pollution when discarded in rivers. The revalorization of coffee by-products is currently being studied due to their high potential as new value-added food ingredients. This work aims to explore the chemical composition and functional properties of coffee pulp and validate its safety for use as a novel food ingredient. Coffee pulp composition was assessed following the standard methods (AOAC). Physicochemical properties, total phenolic compounds (TPC), antioxidant capacity, hypoglycemic and hypolipidemic properties were determined using in vitro techniques. Acute and sub-chronic oral toxicity experiments were carried out following OECD Test Guidelines 452 and 408. Coffee pulp showed a high content of dietary fiber (51.2%), highlighting the insoluble fraction. Proteins (9.2%) and lipids (2.6%) were considered a min fraction. Coffee pulp presented high content of TPC (40.6 mg g−1), of which 29% were linked to DF, giving coffee pulp its antioxidant potential (102.3 mg Trolox eq g−1). The by-product showed good physicochemical properties. Coffee pulp reduced the absorption of cholesterol (84%) and bile salts (85%), inhibited pancreatic lipase (64%), and presented the capacity to diminish the diffusion of glucose (34%). The intake of coffee pulp did not cause significant lesions in vital organs. In conclusion, the coffee pulp could be used as a potential ingredient with beneficial health properties.

ACS Style

Silvia Cañas; Miguel Rebollo-Hernanz; Paz Cano-Muñoz; Yolanda Aguilera; Vanesa Benítez; Cheyenne Braojos; Andrea Gila-Díaz; Pilar Rodríguez-Rodríguez; Ignacio Monedo Cobeta; Ángel L. López de Pablo; Maria Del Carmen González; Silvia M. Arribas; Maria A. Martin-Cabrejas. Critical Evaluation of Coffee Pulp as an Innovative Antioxidant Dietary Fiber Ingredient: Nutritional Value, Functional Properties, and Acute and Sub-Chronic Toxicity. Proceedings 2020, 70, 65 .

AMA Style

Silvia Cañas, Miguel Rebollo-Hernanz, Paz Cano-Muñoz, Yolanda Aguilera, Vanesa Benítez, Cheyenne Braojos, Andrea Gila-Díaz, Pilar Rodríguez-Rodríguez, Ignacio Monedo Cobeta, Ángel L. López de Pablo, Maria Del Carmen González, Silvia M. Arribas, Maria A. Martin-Cabrejas. Critical Evaluation of Coffee Pulp as an Innovative Antioxidant Dietary Fiber Ingredient: Nutritional Value, Functional Properties, and Acute and Sub-Chronic Toxicity. Proceedings. 2020; 70 (1):65.

Chicago/Turabian Style

Silvia Cañas; Miguel Rebollo-Hernanz; Paz Cano-Muñoz; Yolanda Aguilera; Vanesa Benítez; Cheyenne Braojos; Andrea Gila-Díaz; Pilar Rodríguez-Rodríguez; Ignacio Monedo Cobeta; Ángel L. López de Pablo; Maria Del Carmen González; Silvia M. Arribas; Maria A. Martin-Cabrejas. 2020. "Critical Evaluation of Coffee Pulp as an Innovative Antioxidant Dietary Fiber Ingredient: Nutritional Value, Functional Properties, and Acute and Sub-Chronic Toxicity." Proceedings 70, no. 1: 65.

Proceedings
Published: 09 November 2020 in Proceedings
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Obesity is closely associated with the increasing prevalence of non-alcoholic fatty liver disease (NAFLD). Due to the lack of proper pharmacological treatments for NAFLD, finding novel ingredients is necessary to reduce its incidence. Cocoa shell is a cocoa byproduct verified as a safe ingredient and a potential source of health-promoting compounds. Hence, this study’s main objective was to evaluate, after an in vitro simulated digestion, the hypolipidemic properties of the residual fraction of cocoa shell flour and the biological activity of the digested fractions of cocoa shell flour and extract in HepG2 cells. An in vitro static digestion (INFOGEST) of cocoa shell flour was used to establish the residual fraction’s capacity to bind cholesterol and bile salts and inhibit lipase. The results showed that digestion promoted the ability to bind cholesterol and bile salts of a residual fraction from a cocoa shell up to 65.2% and 90.5%. Moreover, digestion improved (1.6-fold, p < 0.05) the ability to inhibit lipase activity. The digested fractions of the flour and extract from the cocoa shell (50–250 µg/mL) significantly (p < 0.05) reduced the accumulation of fat (17–42%), triglycerides (9–38%), and cholesterol (11–54%) in HepG2 cells after NAFLD induction with palmitic acid (500 µM). In conclusion, digestion positively impacted the hypolipidemic properties of cocoa shells in vitro and enhanced their biological activity in cell culture models. Since cocoa shells might be used as a safe, novel ingredient to prevent hyperlipidemia and regulate lipid metabolism, future animal and clinical investigations will be necessary to confirm the effects observed.

ACS Style

Cheyenne Braojos; Vanesa Benitez; Miguel Rebollo-Hernanz; Silvia Cañas; Yolanda Aguilera; Silvia M. Arribas; Maria A. Martin-Cabrejas. Evaluation of the Hypolipidemic Properties of Cocoa Shell after Simulated Digestion Using In Vitro Techniques and a Cell Culture Model of Non-Alcoholic Fatty Liver Disease. Proceedings 2020, 70, 58 .

AMA Style

Cheyenne Braojos, Vanesa Benitez, Miguel Rebollo-Hernanz, Silvia Cañas, Yolanda Aguilera, Silvia M. Arribas, Maria A. Martin-Cabrejas. Evaluation of the Hypolipidemic Properties of Cocoa Shell after Simulated Digestion Using In Vitro Techniques and a Cell Culture Model of Non-Alcoholic Fatty Liver Disease. Proceedings. 2020; 70 (1):58.

Chicago/Turabian Style

Cheyenne Braojos; Vanesa Benitez; Miguel Rebollo-Hernanz; Silvia Cañas; Yolanda Aguilera; Silvia M. Arribas; Maria A. Martin-Cabrejas. 2020. "Evaluation of the Hypolipidemic Properties of Cocoa Shell after Simulated Digestion Using In Vitro Techniques and a Cell Culture Model of Non-Alcoholic Fatty Liver Disease." Proceedings 70, no. 1: 58.

Conference paper
Published: 30 October 2020 in Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease
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Approximately 90% of the coffee cherry is discarded as waste during coffee bean processing. Coffee pulp has been validated as a potential safe ingredient and is a potential source of nutrients and health-promoting compounds that could be used as nutraceuticals to manage some chronic diseases. Metabolic disorders associated with dysregulated energy and cellular processes, such as obesity and hyperlipidemia, contribute to non-alcoholic fatty liver disease (NAFLD). In this sense, the main objective of this study was to evaluate the impact of an in vitro simulated digestion on the hypolipidemic properties of coffee pulp flour and the biological activity of the digested fractions of its flour and extract in HepG2 cells. The hypolipidemic properties of coffee pulp flour were tested by measuring the capacities of the residual fraction of each digestion to bind cholesterol and bile salts and to inhibit the lipase activity after simulated gastric, intestinal, and colonic in vitro digestion. The results exhibited that coffee pulp residual fraction had up to 58% (p < 0.05) more capacity to bind cholesterol, 1.9-fold (p < 0.05) higher bile salts binding capacity, and 1.5-fold (p < 0.05) higher ability to reduce the lipase activity than control residues. Likewise, the digested fractions of coffee pulp flour and extract (50–250 µg/mL) significantly (p < 0.05) alleviated the accumulation of fat (14–35%), triglycerides (5–27%), and cholesterol (9–48%) triggered by the stimulation of HepG2 cells with palmitic acid (500 µM) to simulate NAFLD. In conclusion, simulated gastrointestinal and colonic digestion improves coffee pulp hypolipidemic properties, enhancing its biological activity in cell culture models. Therefore, this coffee by-product could be an interesting potential ingredient to be used to prevent hyperlipidemia and regulate lipid metabolism.

ACS Style

Cheyenne Braojos; Miguel Rebollo-Hernanz; Vanesa Benitez; Silvia Cañas; Yolanda Aguilera; Silvia M. Arribas; Maria A. Martin-Cabrejas. Simulated Gastrointestinal Digestion Influences the In Vitro Hypolipidemic Properties of Coffee Pulp, a Potential Ingredient for the Prevention of Non-Alcoholic Fatty Liver Disease. Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease 2020, 61, 19 .

AMA Style

Cheyenne Braojos, Miguel Rebollo-Hernanz, Vanesa Benitez, Silvia Cañas, Yolanda Aguilera, Silvia M. Arribas, Maria A. Martin-Cabrejas. Simulated Gastrointestinal Digestion Influences the In Vitro Hypolipidemic Properties of Coffee Pulp, a Potential Ingredient for the Prevention of Non-Alcoholic Fatty Liver Disease. Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease. 2020; 61 (1):19.

Chicago/Turabian Style

Cheyenne Braojos; Miguel Rebollo-Hernanz; Vanesa Benitez; Silvia Cañas; Yolanda Aguilera; Silvia M. Arribas; Maria A. Martin-Cabrejas. 2020. "Simulated Gastrointestinal Digestion Influences the In Vitro Hypolipidemic Properties of Coffee Pulp, a Potential Ingredient for the Prevention of Non-Alcoholic Fatty Liver Disease." Proceedings of The 1st International Electronic Conference on Nutrients - Nutritional and Microbiota Effects on Chronic Disease 61, no. 1: 19.

Journal article
Published: 16 October 2020 in Biomedicines
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Fetal undernutrition programs hypertension and cardiovascular diseases, and resistance artery remodeling may be a contributing factor. We aimed to assess if fetal undernutrition induces resistance artery remodeling and the relationship with hypertension. Sprague–Dawley dams were fed ad libitum (Control) or with 50% of control intake between days 11 and 21 of gestation (maternal undernutrition, MUN). In six-month-old male and female offspring we assessed blood pressure (anesthetized and tail-cuff); mesenteric resistance artery (MRA) structure and mechanics (pressure myography), cellular and internal elastic lamina (IEL) organization (confocal microscopy) and plasma MMP-2 and MMP-9 activity (zymography). Systolic blood pressure (SBP, tail-cuff) and plasma MMP activity were assessed in 18-month-old rats. At the age of six months MUN males exhibited significantly higher blood pressure (anesthetized or tail-cuff) and plasma MMP-9 activity, while MUN females did not exhibit significant differences, compared to sex-matched controls. MRA from 6-month-old MUN males and females showed a smaller diameter, reduced adventitial, smooth muscle cell density and IEL fenestra area, and a leftward shift of stress-strain curves. At the age of eighteen months SBP and MMP-9 activity were higher in both MUN males and females, compared to sex-matched controls. These data suggest that fetal undernutrition induces MRA inward eutrophic remodeling and stiffness in both sexes, independent of blood pressure level. Resistance artery structural and mechanical alterations can participate in the development of hypertension in aged females and may contribute to adverse cardiovascular events associated with low birth weight in both sexes.

ACS Style

Perla Y. Gutiérrez-Arzapalo; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Beatriz Somoza; Ángel Luis López De Pablo; Maria Del Carmen González; Silvia M. Arribas. Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension. Biomedicines 2020, 8, 424 .

AMA Style

Perla Y. Gutiérrez-Arzapalo, Pilar Rodríguez-Rodríguez, David Ramiro-Cortijo, Marta Gil-Ortega, Beatriz Somoza, Ángel Luis López De Pablo, Maria Del Carmen González, Silvia M. Arribas. Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension. Biomedicines. 2020; 8 (10):424.

Chicago/Turabian Style

Perla Y. Gutiérrez-Arzapalo; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Beatriz Somoza; Ángel Luis López De Pablo; Maria Del Carmen González; Silvia M. Arribas. 2020. "Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension." Biomedicines 8, no. 10: 424.

Journal article
Published: 30 September 2020 in International Journal of Environmental Research and Public Health
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Early breastfeeding cessation is a major public health problem. Several factors can affect breastfeeding pattern, and psychological aspects have been poorly explored. We hypothesize that psychological factors and breastfeeding pattern have a relationship. We have assessed in mothers during the first six months of lactation if breastfeeding pattern is associated with maternal stress, postpartum depression, and dispositional optimism, and if these psychological factors play a role on breastfeeding adherence. In total, 711 women participated, answering online the following questionnaires: sociodemographic, perceived stress scale, Edinburgh postpartum depression scale, life orientation test, and breastfeeding adherence score. Women were categorized according to infant feeding practices as exclusive breastfeeding (EBF) or mixed breastfeeding (MBF). The EBF group had a lower score of perceived stress compared to those giving MBF (first month: EBF = 1.5 [1.1; 1.9], MBF = 1.8 [1.5; 2.0]; p-Value = 0.030; third month: EBF = 1.6 [1.2; 2.0], MBF = 1.8 [1.5; 2.4]; p-Value = 0.038) and also had a lower score of postpartum depression (third month: EBF = 8.0 [6.0; 11.0], MBF = 11.0 [9.0; 15.0]; p-Value = 0.001). The breastfeeding adherence score showed a positive correlation with maternal perceived stress (first month: ρ = 0.27; p-Value = 0.018), and postpartum depression (third month: ρ = 0.30; p-Value < 0.001), and a negative correlation with maternal dispositional optimism (second month: ρ = −0.20; p-Value = 0.028). MBF was positively associated with breastfeeding adherence score (odd ratio (OR) = 1.4 [1.2–1.6]; p-Value < 0.001) and with postpartum depression (OR = 1.1 [1.0; 1.1]; p-Value = 0.020). In the third month of breastfeeding, women with MBF exhibited higher perceive stress and postpartum depression compared to those with EBF and no difference in dispositional optimism. The maternal psychological aspects are associated with breastfeeding pattern. Evaluation of maternal psychological concerns and providing support to lactating mothers may help improving breastfeeding adherence.

ACS Style

Andrea Gila-Díaz; Gloria Herranz Carrillo; Ángel Luis López De Pablo; Silvia M. Arribas; David Ramiro-Cortijo. Association between Maternal Postpartum Depression, Stress, Optimism, and Breastfeeding Pattern in the First Six Months. International Journal of Environmental Research and Public Health 2020, 17, 7153 .

AMA Style

Andrea Gila-Díaz, Gloria Herranz Carrillo, Ángel Luis López De Pablo, Silvia M. Arribas, David Ramiro-Cortijo. Association between Maternal Postpartum Depression, Stress, Optimism, and Breastfeeding Pattern in the First Six Months. International Journal of Environmental Research and Public Health. 2020; 17 (19):7153.

Chicago/Turabian Style

Andrea Gila-Díaz; Gloria Herranz Carrillo; Ángel Luis López De Pablo; Silvia M. Arribas; David Ramiro-Cortijo. 2020. "Association between Maternal Postpartum Depression, Stress, Optimism, and Breastfeeding Pattern in the First Six Months." International Journal of Environmental Research and Public Health 17, no. 19: 7153.

Journal article
Published: 18 September 2020 in Cytokine
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Male fetal sex associates with higher rates of materno-fetal complications. Inflammation and inadequate vasoactive responses are mechanisms implicated in obstetric complications, and alterations in maternal plasma cytokine profile and nitric oxide (NO) metabolites are potential predictive biomarkers. We aimed to assess if these parameters are influenced by fetal sex. A prospective, observational study was carried out in 85 healthy pregnant women with singleton pregnancies in the first trimester of gestation. A blood sample was extracted at the tenth week of gestation. In plasma, we assessed: 1) cytokines (micro-array): pro-inflammatory (IL1α, IL1 β, IL6, TNFα), anti-inflammatory (IL4, IL10, IL13), and chemoattractant (IL8, MCP1, IFNγ), and 2) NO metabolites (liquid chromatography–tandem mass spectrometry and Griess reaction): L-arginine, ADMA, SDMA, nitrates (NOx). Women with a male fetus (n = 50) exhibited, compared with those with a female (n = 35): higher IL1β (OR = 1.09 with 95% CI: 0.97–1.28), and lower IL13 (OR = 0.93 with 95% CI: 0.87–0.99), and higher plasma NOx (OR = 1.14 with 95% CI: 1.03–1.31). Our data suggest that fetal sex influences maternal plasma cytokine profile and NO in early pregnancy. Women with a male fetus may have a worse capacity to counteract an inflammatory response. They may have better vasodilator capacity, but in the presence of an oxidative environment, a higher nitrosative damage may occur. These data reinforce the need to include sex as variable in predictive models.

ACS Style

David Ramiro-Cortijo; María de la Calle; Rainer Böger; Juliane Hannemann; Nicole Lüneburg; María Rosario López-Giménez; Pilar Rodríguez-Rodríguez; María Ángeles Martín-Cabrejas; Vanesa Benítez; Ángel Luis López de Pablo; María Del Carmen González; Silvia M. Arribas. Male fetal sex is associated with low maternal plasma anti-inflammatory cytokine profile in the first trimester of healthy pregnancies. Cytokine 2020, 136, 155290 .

AMA Style

David Ramiro-Cortijo, María de la Calle, Rainer Böger, Juliane Hannemann, Nicole Lüneburg, María Rosario López-Giménez, Pilar Rodríguez-Rodríguez, María Ángeles Martín-Cabrejas, Vanesa Benítez, Ángel Luis López de Pablo, María Del Carmen González, Silvia M. Arribas. Male fetal sex is associated with low maternal plasma anti-inflammatory cytokine profile in the first trimester of healthy pregnancies. Cytokine. 2020; 136 ():155290.

Chicago/Turabian Style

David Ramiro-Cortijo; María de la Calle; Rainer Böger; Juliane Hannemann; Nicole Lüneburg; María Rosario López-Giménez; Pilar Rodríguez-Rodríguez; María Ángeles Martín-Cabrejas; Vanesa Benítez; Ángel Luis López de Pablo; María Del Carmen González; Silvia M. Arribas. 2020. "Male fetal sex is associated with low maternal plasma anti-inflammatory cytokine profile in the first trimester of healthy pregnancies." Cytokine 136, no. : 155290.

Short communication
Published: 17 September 2020 in Pregnancy Hypertension
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Twin pregnancies are considered a risk factor for preeclampsia, an obstetric complication with high maternal and infant morbi-mortality. We hypothesize that alterations in maternal hematocrit, plasma lipid peroxidation and nitrates in the first trimester of pregnancy are associated with preeclampsia development in twin pregnancies. Blood samples were extracted from 102 healthy women with twin pregnancies at tenth week of gestation to assess hematological parameters and plasma levels of malondialdehyde and nitrates. Logistic regression model showed an association between red blood cells (OR = 38.8; p-value = 0.009), hematocrit (OR = 1.6; p-value = 0.017), malondialdehyde (OR = 1.5; p-value = 0.002), and nitrates (OR = 1.1; p-value = 0.045) and preeclampsia development. These parameters are potential biomarkers for early preeclampsia detection in twin pregnancies. Future research is needed to assess their value in predictive algorithms.

ACS Style

David Ramiro-Cortijo; María de la Calle; Pilar Rodriguez-Rodriguez; Sophida Phuthong; Ángel L. López de Pablo; Maria A. Martín-Cabrejas; Silvia M. Arribas. First trimester elevations of hematocrit, lipid peroxidation and nitrates in women with twin pregnancies who develop preeclampsia. Pregnancy Hypertension 2020, 22, 132 -135.

AMA Style

David Ramiro-Cortijo, María de la Calle, Pilar Rodriguez-Rodriguez, Sophida Phuthong, Ángel L. López de Pablo, Maria A. Martín-Cabrejas, Silvia M. Arribas. First trimester elevations of hematocrit, lipid peroxidation and nitrates in women with twin pregnancies who develop preeclampsia. Pregnancy Hypertension. 2020; 22 ():132-135.

Chicago/Turabian Style

David Ramiro-Cortijo; María de la Calle; Pilar Rodriguez-Rodriguez; Sophida Phuthong; Ángel L. López de Pablo; Maria A. Martín-Cabrejas; Silvia M. Arribas. 2020. "First trimester elevations of hematocrit, lipid peroxidation and nitrates in women with twin pregnancies who develop preeclampsia." Pregnancy Hypertension 22, no. : 132-135.

Journal article
Published: 25 August 2020 in Nutrients
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Breast milk (BM) is beneficial due to its content in a wide range of different antioxidants, particularly relevant for preterm infants, who are at higher risk of oxidative stress. We hypothesize that BM antioxidants are adapted to gestational age and are negatively influenced by maternal age. Fifty breastfeeding women from two hospitals (Madrid, Spain) provided BM samples at days 7, 14 and 28 of lactation to assess total antioxidant capacity (ABTS), thiol groups, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities, lipid peroxidation (malondialdehyde, MDA + 4-Hydroxy-Trans-2-Nonenal, HNE), protein oxidation (carbonyl groups) (spectrophotometry) and melatonin (ELISA). Mixed random-effects linear regression models were used to study the influence of maternal and gestational ages on BM antioxidants, adjusted by days of lactation. Regression models evidenced a negative association between maternal age and BM melatonin levels (β = −7.4 ± 2.5; p-value = 0.005); and a negative association between gestational age and BM total antioxidant capacity (β = −0.008 ± 0.003; p-value = 0.006), SOD activity (β = −0.002 ± 0.001; p-value = 0.043) and protein oxidation (β = −0.22 ± 0.07; p-value = 0.001). In conclusion, BM antioxidants are adapted to gestational age providing higher levels to infants with lower degree of maturation; maternal ageing has a negative influence on melatonin, a key antioxidant hormone.

ACS Style

Andrea Gila-Díaz; Gloria Herranz Carrillo; Silvia Cañas; Miguel Saenz De Pipaón; José Antonio Martínez-Orgado; Pilar Rodríguez-Rodríguez; Ángel Luis López De Pablo; María A. Martin-Cabrejas; David Ramiro-Cortijo; Silvia M. Arribas. Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation. Nutrients 2020, 12, 2569 .

AMA Style

Andrea Gila-Díaz, Gloria Herranz Carrillo, Silvia Cañas, Miguel Saenz De Pipaón, José Antonio Martínez-Orgado, Pilar Rodríguez-Rodríguez, Ángel Luis López De Pablo, María A. Martin-Cabrejas, David Ramiro-Cortijo, Silvia M. Arribas. Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation. Nutrients. 2020; 12 (9):2569.

Chicago/Turabian Style

Andrea Gila-Díaz; Gloria Herranz Carrillo; Silvia Cañas; Miguel Saenz De Pipaón; José Antonio Martínez-Orgado; Pilar Rodríguez-Rodríguez; Ángel Luis López De Pablo; María A. Martin-Cabrejas; David Ramiro-Cortijo; Silvia M. Arribas. 2020. "Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation." Nutrients 12, no. 9: 2569.

Journal article
Published: 03 June 2020 in Nutrients
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We aimed to design and validate a new questionnaire of adherence to healthy food pyramid (HFP) (AP-Q), to improve previous instruments. The questionnaire was self-administered and included 28 questions from 10 categories (physical activity, health habits, hydration, grains, fruits, vegetables, oil type, dairy products, animal proteins, and snacks). A population of 130 Spanish adults answered it, obtaining scores from each category and a global score of HFP adherence (AP-Q score). Validation was performed through principal components analysis (PCA) and internal consistency by Cronbach’s alpha. AP-Q was also externally validated with Kidmed-test, answered by 45 individuals from the cohort. The global AP-Q score was 5.1 ± 1.3, with an internal consistency of 64%. The PCA analysis extracted seven principal components, which explained 68.5% of the variance. The global AP-Q score was positively associated with Kidmed-test score. Our data suggest that AP-Q is a complete and robust questionnaire to assess HFP adherence, with several advantages: easy to complete, cost-effective, timesaving and has the competency to assess, besides diet, several features affecting health status, lacking in other instruments. We suggest that AP-Q could be useful in epidemiological research, although it requires additional calibration to analyze its reproducibility and validation in other populations.

ACS Style

Andrea Gila-Díaz; Silvia M. Arribas; Ángel Luis López De Pablo; Ma Rosario López-Giménez; Sophida Phuthong; David Ramiro-Cortijo. Development and Validation of a Questionnaire to Assess Adherence to the Healthy Food Pyramid in Spanish Adults. Nutrients 2020, 12, 1656 .

AMA Style

Andrea Gila-Díaz, Silvia M. Arribas, Ángel Luis López De Pablo, Ma Rosario López-Giménez, Sophida Phuthong, David Ramiro-Cortijo. Development and Validation of a Questionnaire to Assess Adherence to the Healthy Food Pyramid in Spanish Adults. Nutrients. 2020; 12 (6):1656.

Chicago/Turabian Style

Andrea Gila-Díaz; Silvia M. Arribas; Ángel Luis López De Pablo; Ma Rosario López-Giménez; Sophida Phuthong; David Ramiro-Cortijo. 2020. "Development and Validation of a Questionnaire to Assess Adherence to the Healthy Food Pyramid in Spanish Adults." Nutrients 12, no. 6: 1656.

Journal article
Published: 25 March 2020 in Antioxidants
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Twin pregnancies are increasing due to the rise in mothers’ childbearing age and have a higher risk of fetal growth restriction (FGR) and prematurity. Therefore, early prediction of these events is important. Our aim was to analyze in the first trimester of pregnancy a possible association between antioxidants, including melatonin, in maternal plasma and the development of fetal complications in twin pregnancies. A single-center, prospective, and observational study was performed in 104 twin-pregnant women. A blood sample was extracted between the 9th and the 11th week of gestation, and plasma was obtained. Antioxidants (thiols, reduced glutathione, phenolic compounds, catalase, superoxide dismutase) and oxidative damage biomarkers (carbonyl groups and malondialdehyde) were assessed by spectrophotometry, and global scores were calculated from these parameters (Antiox-S, Prooxy-S). Melatonin and cortisol were evaluated by a competitive immunoassay. In the first trimester of pregnancy, Antiox-S was significantly lower in women who developed FGR compared to those with normal fetal growth; plasma melatonin was significantly lower in women with preterm compared to those with full-term births and exhibited a positive correlation with birth weight. Maternal cortisol showed a negative correlation with birth weight. We conclude that, for twin gestations, maternal plasma antioxidant status and melatonin could be potential biomarkers to be included in algorithms to predict FGR and preterm labor.

ACS Style

David Ramiro-Cortijo; María De La Calle; Pilar Rodríguez-Rodríguez; Ángel L. López De Pablo; María R. López-Giménez; Yolanda Aguilera; María A. Martín-Cabrejas; María Del Carmen González; Silvia M. Arribas. Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study. Antioxidants 2020, 9, 269 .

AMA Style

David Ramiro-Cortijo, María De La Calle, Pilar Rodríguez-Rodríguez, Ángel L. López De Pablo, María R. López-Giménez, Yolanda Aguilera, María A. Martín-Cabrejas, María Del Carmen González, Silvia M. Arribas. Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study. Antioxidants. 2020; 9 (4):269.

Chicago/Turabian Style

David Ramiro-Cortijo; María De La Calle; Pilar Rodríguez-Rodríguez; Ángel L. López De Pablo; María R. López-Giménez; Yolanda Aguilera; María A. Martín-Cabrejas; María Del Carmen González; Silvia M. Arribas. 2020. "Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study." Antioxidants 9, no. 4: 269.

Journal article
Published: 12 March 2020 in Foods
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Melatonin is a multifunctional antioxidant neurohormone found in plant foods such as lentil sprouts. We aim to evaluate the effect of lentil sprout intake on the plasmatic levels of melatonin and metabolically related compounds (plasmatic serotonin and urinary 6-sulfatoxymelatonin), total phenolic compounds, and plasmatic antioxidant status, and compare it with synthetic melatonin. The germination of lentils increases the content of melatonin. However, the phenolic content diminished due to the loss of phenolic acids and flavan-3-ols. The flavonol content remained unaltered, being the main phenolic family in lentil sprouts, primarily composed of kaempferol glycosides. Sprague Dawley rats were used to investigate the pharmacokinetic profile of melatonin after oral administration of a lentil sprout extract and to evaluate plasma and urine melatonin and related biomarkers and antioxidant capacity. Melatonin showed maximum concentration (45.4 pg/mL) 90 min after lentil sprout administration. The plasmatic melatonin levels increased after lentil sprout intake (70%, p < 0.05) with respect to the control, 1.2-fold more than after synthetic melatonin ingestion. These increments correlated with urinary 6-sulfatoxymelatonin content (p < 0.05), a key biomarker of plasmatic melatonin. Nonetheless, the phenolic compound content did not exhibit any significant variation. Plasmatic antioxidant status increased in the antioxidant capacity upon both lentil sprout and synthetic melatonin administration. For the first time, we investigated the bioavailability of melatonin from lentil sprouts and its role in plasmatic antioxidant status. We concluded that their intake could increase melatonin plasmatic concentration and attenuate plasmatic oxidative stress.

ACS Style

Miguel Rebollo-Hernanz; Yolanda Aguilera; Teresa Herrera; L. Tábata Cayuelas; Montserrat Dueñas; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Silvia M. Arribas; María A. Martín-Cabrejas. Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats. Foods 2020, 9, 330 .

AMA Style

Miguel Rebollo-Hernanz, Yolanda Aguilera, Teresa Herrera, L. Tábata Cayuelas, Montserrat Dueñas, Pilar Rodríguez-Rodríguez, David Ramiro-Cortijo, Silvia M. Arribas, María A. Martín-Cabrejas. Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats. Foods. 2020; 9 (3):330.

Chicago/Turabian Style

Miguel Rebollo-Hernanz; Yolanda Aguilera; Teresa Herrera; L. Tábata Cayuelas; Montserrat Dueñas; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Silvia M. Arribas; María A. Martín-Cabrejas. 2020. "Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats." Foods 9, no. 3: 330.