This page has only limited features, please log in for full access.
Adolescent pregnancy remains a health issue worldwide also in developed countries, since it has been associated with adverse maternal and neonatal outcomes. Some data suggest that very young adolescents have higher risk, likely due to immaturity. Therefore, we aimed to assess the influence of maternal age on complications during gestation and labor in pregnant women between 13 and 19 years of age. In particular, we evaluated the possible association between maternal age and obstetric, fetal and labor complications. This is a retrospective, observational and exploratory study conducted at Hospital Universitario La Paz (HULP, Madrid, Spain). The clinical history of 279 women who delivered between 2013 and 2018 was analyzed. Maternal age and the presence of maternal, fetal and labor complications, as well as risk of postpartum depression and breastfeeding intention, were recorded. General regression models were used to analyze the contribution of maternal age on each complication. The percentage of adolescent pregnancies at HULP between 2013 and 2018 was 0.9%. The risk of all the maternal complications analyzed decreased significantly with every year of age of the mother (hyperemesis, lower back pain, anemia, gestational diabetes mellitus, and threat of premature labor and premature rupture of membranes). Every year of maternal age decreased 0.8-fold [0.8; 0.9] the prevalence of fetal complications and also reduced the risk of C-section, postpartum hemorrhage and obstetrical hysterectomy. Furthermore, higher maternal age increased 1.1-fold [1.0; 1.2] the breastfeeding intention. In conclusion, young adolescents are at higher risk of complications during pregnancy and labor.
Maria de la Calle; Jose L. Bartha; Cristina M. Lopez; Miriam Turiel; Nuria Martinez; Silvia M. Arribas; David Ramiro-Cortijo. Younger Age in Adolescent Pregnancies Is Associated with Higher Risk of Adverse Outcomes. International Journal of Environmental Research and Public Health 2021, 18, 8514 .
AMA StyleMaria de la Calle, Jose L. Bartha, Cristina M. Lopez, Miriam Turiel, Nuria Martinez, Silvia M. Arribas, David Ramiro-Cortijo. Younger Age in Adolescent Pregnancies Is Associated with Higher Risk of Adverse Outcomes. International Journal of Environmental Research and Public Health. 2021; 18 (16):8514.
Chicago/Turabian StyleMaria de la Calle; Jose L. Bartha; Cristina M. Lopez; Miriam Turiel; Nuria Martinez; Silvia M. Arribas; David Ramiro-Cortijo. 2021. "Younger Age in Adolescent Pregnancies Is Associated with Higher Risk of Adverse Outcomes." International Journal of Environmental Research and Public Health 18, no. 16: 8514.
Cardiovascular disease remains a leading cause of mortality worldwide. Unresolved inflammation plays a critical role in cardiovascular diseases development. Specialized Pro-Resolving Mediators (SPMs), derived from long chain polyunsaturated fatty acids (LCPUFAs), enhances the host defense, by resolving the inflammation and tissue repair. In addition, SPMs also have anti-inflammatory properties. These physiological effects depend on the availability of LCPUFAs precursors and cellular metabolic balance. Most of the studies have focused on the impact of SPMs in adult cardiovascular health and diseases. In this review, we discuss LCPUFAs metabolism, SPMs, and their potential effect on cardiovascular health and diseases primarily focusing in neonates. A better understanding of the role of these SPMs in cardiovascular health and diseases in neonates could lead to the development of novel therapeutic approaches in cardiovascular dysfunction.
Andrea Gila-Diaz; Gloria Carrillo; Pratibha Singh; David Ramiro-Cortijo. Specialized Pro-Resolving Lipid Mediators in Neonatal Cardiovascular Physiology and Diseases. Antioxidants 2021, 10, 933 .
AMA StyleAndrea Gila-Diaz, Gloria Carrillo, Pratibha Singh, David Ramiro-Cortijo. Specialized Pro-Resolving Lipid Mediators in Neonatal Cardiovascular Physiology and Diseases. Antioxidants. 2021; 10 (6):933.
Chicago/Turabian StyleAndrea Gila-Diaz; Gloria Carrillo; Pratibha Singh; David Ramiro-Cortijo. 2021. "Specialized Pro-Resolving Lipid Mediators in Neonatal Cardiovascular Physiology and Diseases." Antioxidants 10, no. 6: 933.
The first month of lactation is a vulnerable nutritional period for the mother. Our aims were (1) to evaluate the nutritional status of breastfeeding women in the first month of lactation, and (2) to explore different aspects of nutrition and lifestyle through a multidimensional approach. A total of 30 healthy breastfeeding women were enrolled in this study. Dietary pattern was assessed through a 72-hour dietary recall questionnaire (days 7 and 28 postpartum) and data were compared with Dietary Recommendation Values (DRV), and through the Adherence to Healthy Food Pyramid (HFP) questionnaire (day 28). Anthropometric parameters were evaluated by bioimpedance. Using factor analysis, nutritional dimensions were extracted, and linear regression models were used to analyze the association between anthropometric parameters and dimensions. Compared to the DRV, women showed insufficient energy, water, vitamin D, and potassium intake and excessive proteins, vitamins B1, B2, B3, B6, B12, and iron intake. We observed a moderate adherence to the HFP, with women being high in the fruits, vegetables, and oil categories, and low adherence to the physical activity, dairy products, and hydration categories. The nutritional dimension, including the HFP categories of physical activity, hydration, and animal protein intake together, was negatively associated with body weight (β = −3.7 ± 1.7; p-value = 0.047). In conclusion, during the first month postpartum, breastfeeding women exhibited several nutritional imbalances and poor physical activity negatively influencing anthropometric parameters. We propose a multidimensional approach to assess the nutritional status of breastfeeding women as a tool to detect specific deficiencies, allowing for personalized counseling.
Andrea Gila-Díaz; Nuria Díaz-Rullo Alcántara; Gloria Herranz Carrillo; Pratibha Singh; Silvia Arribas; David Ramiro-Cortijo. Multidimensional Approach to Assess Nutrition and Lifestyle in Breastfeeding Women during the First Month of Lactation. Nutrients 2021, 13, 1766 .
AMA StyleAndrea Gila-Díaz, Nuria Díaz-Rullo Alcántara, Gloria Herranz Carrillo, Pratibha Singh, Silvia Arribas, David Ramiro-Cortijo. Multidimensional Approach to Assess Nutrition and Lifestyle in Breastfeeding Women during the First Month of Lactation. Nutrients. 2021; 13 (6):1766.
Chicago/Turabian StyleAndrea Gila-Díaz; Nuria Díaz-Rullo Alcántara; Gloria Herranz Carrillo; Pratibha Singh; Silvia Arribas; David Ramiro-Cortijo. 2021. "Multidimensional Approach to Assess Nutrition and Lifestyle in Breastfeeding Women during the First Month of Lactation." Nutrients 13, no. 6: 1766.
Early detection of gestational complications is a priority in obstetrics. In our social context, this is linked to maternity age. Most studies are focused on biological factors. However, pregnancy is also influenced by social and psychological factors, which have not been deeply explored. We aimed to identify biopsychosocial risk and protective factors associated with the development of maternal and fetal complications. We enrolled 182 healthy pregnant women, and plasma melatonin and cortisol levels were measured in the first trimester by chemiluminescent immunoassays. At different time points along gestation, women answered several questionnaires (positive and negative affect schedule, hospital anxiety and depression scale, pregnancy concerns scale, life orientation test, resilience scale, life satisfaction scale and life–work conflicts scale). They were followed up until delivery and categorized as normal pregnancy, maternal or fetal complications. Maternal complications were associated with low melatonin (OR = 0.99 [0.98; 1.00]; p-value = 0.08) and life satisfaction (OR = 0.64 [0.41; 0.93]; p-value = 0.03) and fetal complications were associated with high cortisol (OR = 1.06 [1.02; 1.13]; p-value = 0.04), anxiety (OR = 2.21 [1.10; 4.55]; p-value = 0.03) and life–work conflicts (OR = 1.92 [1.04; 3.75]; p-value = 0.05). We conclude that psychological factors influence pregnancy outcomes in association with melatonin and cortisol alterations. High maternal melatonin and life satisfaction levels could be potential protective factors against the development of maternal complications during pregnancy. Low anxiety and cortisol levels and reduced work–life conflicts could prevent fetal complications.
David Ramiro-Cortijo; Maria de la Calle; Vanesa Benitez; Andrea Gila-Diaz; Bernardo Moreno-Jiménez; Silvia Arribas; Eva Garrosa. Maternal Psychological and Biological Factors Associated to Gestational Complications. Journal of Personalized Medicine 2021, 11, 183 .
AMA StyleDavid Ramiro-Cortijo, Maria de la Calle, Vanesa Benitez, Andrea Gila-Diaz, Bernardo Moreno-Jiménez, Silvia Arribas, Eva Garrosa. Maternal Psychological and Biological Factors Associated to Gestational Complications. Journal of Personalized Medicine. 2021; 11 (3):183.
Chicago/Turabian StyleDavid Ramiro-Cortijo; Maria de la Calle; Vanesa Benitez; Andrea Gila-Diaz; Bernardo Moreno-Jiménez; Silvia Arribas; Eva Garrosa. 2021. "Maternal Psychological and Biological Factors Associated to Gestational Complications." Journal of Personalized Medicine 11, no. 3: 183.
Cognitive maternal adaptation during pregnancy may influence biological variables, maternal psychological, and neonatal health. We hypothesized that pregnant women with numerous general resources and less negative emotions would have a better coping with a positive influence on neonatal birth weight and maternal psychological health. The study included 131 healthy pregnant women. A blood sample was obtained in the first trimester to assess biological variables (polyphenols, hematological and biochemical parameters). Psychological variables (negative affect, anxiety, optimism, resilience, family–work conflicts, pregnancy concerns, general resources, and life satisfaction) were evaluated at several time points along gestation, and birth weight was recorded. Hierarchical linear regression models were used to associate the above parameters with maternal psychological outcome at the end of gestation (depression, resilience, and optimism) and neonatal outcome (birth weight). Maternal depression was associated with leukocytes (β = 0.08 ± 0.03, p-value = 0.003), cholesterol (β = 0.01 ± 0.002, p-value = 0.026), and pregnancy concerns (β = 0.31 ± 0.09, p-value = 0.001). Maternal resilience was associated with leukocytes (β = −0.14 ± 0.09, p-value = 0.010) and life satisfaction (β = 0.82 ± 0.08, p-value = 0.001), and maternal optimism was associated with polyphenol levels (β = 0.002 ± 0.001, p-value = 0.018) and life satisfaction (β = 0.49 ± 0.04, p-value = 0.001). Birth weight was associated with maternal resilience (β = 370.2 ± 97.0, p-value = 0.001), red blood cells (β = 480.3 ± 144.4, p-value = 0.001), and life satisfaction (β = 423.3 ± 32.6, p-value = 0.001). We found associations between maternal psychological, blood variables, and birth weight and maternal depression. This study reveals the relevance of psychological health during pregnancy for maternal and neonatal outcome, and it emphasizes the need to consider it in preventive policies in the obstetric field.
David Ramiro-Cortijo; María de la Calle; Andrea Gila-Díaz; Bernardo Moreno-Jiménez; Maria Martin-Cabrejas; Silvia Arribas; Eva Garrosa. Maternal Resources, Pregnancy Concerns, and Biological Factors Associated to Birth Weight and Psychological Health. Journal of Clinical Medicine 2021, 10, 695 .
AMA StyleDavid Ramiro-Cortijo, María de la Calle, Andrea Gila-Díaz, Bernardo Moreno-Jiménez, Maria Martin-Cabrejas, Silvia Arribas, Eva Garrosa. Maternal Resources, Pregnancy Concerns, and Biological Factors Associated to Birth Weight and Psychological Health. Journal of Clinical Medicine. 2021; 10 (4):695.
Chicago/Turabian StyleDavid Ramiro-Cortijo; María de la Calle; Andrea Gila-Díaz; Bernardo Moreno-Jiménez; Maria Martin-Cabrejas; Silvia Arribas; Eva Garrosa. 2021. "Maternal Resources, Pregnancy Concerns, and Biological Factors Associated to Birth Weight and Psychological Health." Journal of Clinical Medicine 10, no. 4: 695.
Multicomponent lipid emulsions are available for critical care of preterm infants. We sought to determine the impact of different lipid emulsions on early priming of the host and its response to an acute stimulus. Pigs delivered 7d preterm (n = 59) were randomized to receive different lipid emulsions for 11 days: 100% soybean oil (SO), mixed oil emulsion (SO, medium chain olive oil and fish oil) including 15% fish oil (MO15), or 100% fish oil (FO100). On day 11, pigs received an 8-h continuous intravenous infusion of either lipopolysaccharide (LPS—lyophilized Escherichia coli) or saline. Plasma was collected for fatty acid, oxylipin, metabolomic, and cytokine analyses. At day 11, plasma omega-3 fatty acid levels in the FO100 groups showed the highest increase in eicosapentaenoic acid, EPA (0.1 ± 0.0 to 9.7 ± 1.9, p < 0.001), docosahexaenoic acid, DHA (day 0 = 2.5 ± 0.7 to 13.6 ± 2.9, p < 0.001), EPA and DHA-derived oxylipins, and sphingomyelin metabolites. In the SO group, levels of cytokine IL1β increased at the first hour of LPS infusion (296.6 ± 308 pg/mL) but was undetectable in MO15, FO100, or in the animals receiving saline instead of LPS. Pigs in the SO group showed a significant increase in arachidonic acid (AA)-derived prostaglandins and thromboxanes in the first hour (p < 0.05). No significant changes in oxylipins were observed with either fish-oil containing group during LPS infusion. Host priming with soybean oil in the early postnatal period preserves a higher AA:DHA ratio and the ability to acutely respond to an external stimulus. In contrast, fish-oil containing lipid emulsions increase DHA, exacerbate a deficit in AA, and limit the initial LPS-induced inflammatory responses in preterm pigs.
William Yakah; David Ramiro-Cortijo; Pratibha Singh; Joanne Brown; Barbara Stoll; Madhulika Kulkarni; Berthe C. Oosterloo; Doug Burrin; Krishna Rao Maddipati; Raina N. Fichorova; Steven D. Freedman; Camilia R. Martin. Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs. Nutrients 2021, 13, 205 .
AMA StyleWilliam Yakah, David Ramiro-Cortijo, Pratibha Singh, Joanne Brown, Barbara Stoll, Madhulika Kulkarni, Berthe C. Oosterloo, Doug Burrin, Krishna Rao Maddipati, Raina N. Fichorova, Steven D. Freedman, Camilia R. Martin. Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs. Nutrients. 2021; 13 (1):205.
Chicago/Turabian StyleWilliam Yakah; David Ramiro-Cortijo; Pratibha Singh; Joanne Brown; Barbara Stoll; Madhulika Kulkarni; Berthe C. Oosterloo; Doug Burrin; Krishna Rao Maddipati; Raina N. Fichorova; Steven D. Freedman; Camilia R. Martin. 2021. "Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs." Nutrients 13, no. 1: 205.
Fetal undernutrition programs cardiometabolic diseases, with higher susceptibility in males. The mechanisms implicated are not fully understood and may be related to sex differences in placental adaptation. To evaluate this hypothesis, we investigated placental oxidative balance, vascularization, glucocorticoid barrier, and fetal growth in rats exposed to 50% global nutrient restriction from gestation day 11 (MUN, n = 8) and controls (n = 8). At gestation day 20 (G20), we analyzed maternal, placental, and fetal weights; oxidative damage, antioxidants, corticosterone, and PlGF (placental growth factor, spectrophotometry); and VEGF (vascular endothelial growth factor), 11β-HSD2, p22phox, XO, SOD1, SOD2, SOD3, catalase, and UCP2 expression (Western blot). Compared with controls, MUN dams exhibited lower weight and plasma proteins and higher corticosterone and catalase without oxidative damage. Control male fetuses were larger than female fetuses. MUN males had higher plasma corticosterone and were smaller than control males, but had similar weight than MUN females. MUN male placenta showed higher XO and lower 11β-HSD2, VEGF, SOD2, catalase, UCP2, and feto-placental ratio than controls. MUN females had similar feto-placental ratio and plasma corticosterone than controls. Female placenta expressed lower XO, 11β-HSD2, and SOD3; similar VEGF, SOD1, SOD2, and UCP2; and higher catalase than controls, being 11β-HSD2 and VEGF higher compared to MUN males. Male placenta has worse adaptation to undernutrition with lower efficiency, associated with oxidative disbalance and reduced vascularization and glucocorticoid barrier. Glucocorticoids and low nutrients may both contribute to programming in MUN males.
Sophida Phuthong; Cynthia Guadalupe Reyes-Hernández; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Raquel González-Blázquez; M. Carmen González; Angel Luis López De Pablo; Silvia M. Arribas. Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition. International Journal of Molecular Sciences 2020, 22, 237 .
AMA StyleSophida Phuthong, Cynthia Guadalupe Reyes-Hernández, Pilar Rodríguez-Rodríguez, David Ramiro-Cortijo, Marta Gil-Ortega, Raquel González-Blázquez, M. Carmen González, Angel Luis López De Pablo, Silvia M. Arribas. Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition. International Journal of Molecular Sciences. 2020; 22 (1):237.
Chicago/Turabian StyleSophida Phuthong; Cynthia Guadalupe Reyes-Hernández; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Raquel González-Blázquez; M. Carmen González; Angel Luis López De Pablo; Silvia M. Arribas. 2020. "Sex Differences in Placental Protein Expression and Efficiency in a Rat Model of Fetal Programming Induced by Maternal Undernutrition." International Journal of Molecular Sciences 22, no. 1: 237.
Fetal undernutrition programs hypertension and cardiovascular diseases, and resistance artery remodeling may be a contributing factor. We aimed to assess if fetal undernutrition induces resistance artery remodeling and the relationship with hypertension. Sprague–Dawley dams were fed ad libitum (Control) or with 50% of control intake between days 11 and 21 of gestation (maternal undernutrition, MUN). In six-month-old male and female offspring we assessed blood pressure (anesthetized and tail-cuff); mesenteric resistance artery (MRA) structure and mechanics (pressure myography), cellular and internal elastic lamina (IEL) organization (confocal microscopy) and plasma MMP-2 and MMP-9 activity (zymography). Systolic blood pressure (SBP, tail-cuff) and plasma MMP activity were assessed in 18-month-old rats. At the age of six months MUN males exhibited significantly higher blood pressure (anesthetized or tail-cuff) and plasma MMP-9 activity, while MUN females did not exhibit significant differences, compared to sex-matched controls. MRA from 6-month-old MUN males and females showed a smaller diameter, reduced adventitial, smooth muscle cell density and IEL fenestra area, and a leftward shift of stress-strain curves. At the age of eighteen months SBP and MMP-9 activity were higher in both MUN males and females, compared to sex-matched controls. These data suggest that fetal undernutrition induces MRA inward eutrophic remodeling and stiffness in both sexes, independent of blood pressure level. Resistance artery structural and mechanical alterations can participate in the development of hypertension in aged females and may contribute to adverse cardiovascular events associated with low birth weight in both sexes.
Perla Y. Gutiérrez-Arzapalo; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Beatriz Somoza; Ángel Luis López De Pablo; Maria Del Carmen González; Silvia M. Arribas. Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension. Biomedicines 2020, 8, 424 .
AMA StylePerla Y. Gutiérrez-Arzapalo, Pilar Rodríguez-Rodríguez, David Ramiro-Cortijo, Marta Gil-Ortega, Beatriz Somoza, Ángel Luis López De Pablo, Maria Del Carmen González, Silvia M. Arribas. Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension. Biomedicines. 2020; 8 (10):424.
Chicago/Turabian StylePerla Y. Gutiérrez-Arzapalo; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Marta Gil-Ortega; Beatriz Somoza; Ángel Luis López De Pablo; Maria Del Carmen González; Silvia M. Arribas. 2020. "Fetal Undernutrition Induces Resistance Artery Remodeling and Stiffness in Male and Female Rats Independent of Hypertension." Biomedicines 8, no. 10: 424.
Early breastfeeding cessation is a major public health problem. Several factors can affect breastfeeding pattern, and psychological aspects have been poorly explored. We hypothesize that psychological factors and breastfeeding pattern have a relationship. We have assessed in mothers during the first six months of lactation if breastfeeding pattern is associated with maternal stress, postpartum depression, and dispositional optimism, and if these psychological factors play a role on breastfeeding adherence. In total, 711 women participated, answering online the following questionnaires: sociodemographic, perceived stress scale, Edinburgh postpartum depression scale, life orientation test, and breastfeeding adherence score. Women were categorized according to infant feeding practices as exclusive breastfeeding (EBF) or mixed breastfeeding (MBF). The EBF group had a lower score of perceived stress compared to those giving MBF (first month: EBF = 1.5 [1.1; 1.9], MBF = 1.8 [1.5; 2.0]; p-Value = 0.030; third month: EBF = 1.6 [1.2; 2.0], MBF = 1.8 [1.5; 2.4]; p-Value = 0.038) and also had a lower score of postpartum depression (third month: EBF = 8.0 [6.0; 11.0], MBF = 11.0 [9.0; 15.0]; p-Value = 0.001). The breastfeeding adherence score showed a positive correlation with maternal perceived stress (first month: ρ = 0.27; p-Value = 0.018), and postpartum depression (third month: ρ = 0.30; p-Value < 0.001), and a negative correlation with maternal dispositional optimism (second month: ρ = −0.20; p-Value = 0.028). MBF was positively associated with breastfeeding adherence score (odd ratio (OR) = 1.4 [1.2–1.6]; p-Value < 0.001) and with postpartum depression (OR = 1.1 [1.0; 1.1]; p-Value = 0.020). In the third month of breastfeeding, women with MBF exhibited higher perceive stress and postpartum depression compared to those with EBF and no difference in dispositional optimism. The maternal psychological aspects are associated with breastfeeding pattern. Evaluation of maternal psychological concerns and providing support to lactating mothers may help improving breastfeeding adherence.
Andrea Gila-Díaz; Gloria Herranz Carrillo; Ángel Luis López De Pablo; Silvia M. Arribas; David Ramiro-Cortijo. Association between Maternal Postpartum Depression, Stress, Optimism, and Breastfeeding Pattern in the First Six Months. International Journal of Environmental Research and Public Health 2020, 17, 7153 .
AMA StyleAndrea Gila-Díaz, Gloria Herranz Carrillo, Ángel Luis López De Pablo, Silvia M. Arribas, David Ramiro-Cortijo. Association between Maternal Postpartum Depression, Stress, Optimism, and Breastfeeding Pattern in the First Six Months. International Journal of Environmental Research and Public Health. 2020; 17 (19):7153.
Chicago/Turabian StyleAndrea Gila-Díaz; Gloria Herranz Carrillo; Ángel Luis López De Pablo; Silvia M. Arribas; David Ramiro-Cortijo. 2020. "Association between Maternal Postpartum Depression, Stress, Optimism, and Breastfeeding Pattern in the First Six Months." International Journal of Environmental Research and Public Health 17, no. 19: 7153.
Male fetal sex associates with higher rates of materno-fetal complications. Inflammation and inadequate vasoactive responses are mechanisms implicated in obstetric complications, and alterations in maternal plasma cytokine profile and nitric oxide (NO) metabolites are potential predictive biomarkers. We aimed to assess if these parameters are influenced by fetal sex. A prospective, observational study was carried out in 85 healthy pregnant women with singleton pregnancies in the first trimester of gestation. A blood sample was extracted at the tenth week of gestation. In plasma, we assessed: 1) cytokines (micro-array): pro-inflammatory (IL1α, IL1 β, IL6, TNFα), anti-inflammatory (IL4, IL10, IL13), and chemoattractant (IL8, MCP1, IFNγ), and 2) NO metabolites (liquid chromatography–tandem mass spectrometry and Griess reaction): L-arginine, ADMA, SDMA, nitrates (NOx). Women with a male fetus (n = 50) exhibited, compared with those with a female (n = 35): higher IL1β (OR = 1.09 with 95% CI: 0.97–1.28), and lower IL13 (OR = 0.93 with 95% CI: 0.87–0.99), and higher plasma NOx (OR = 1.14 with 95% CI: 1.03–1.31). Our data suggest that fetal sex influences maternal plasma cytokine profile and NO in early pregnancy. Women with a male fetus may have a worse capacity to counteract an inflammatory response. They may have better vasodilator capacity, but in the presence of an oxidative environment, a higher nitrosative damage may occur. These data reinforce the need to include sex as variable in predictive models.
David Ramiro-Cortijo; María de la Calle; Rainer Böger; Juliane Hannemann; Nicole Lüneburg; María Rosario López-Giménez; Pilar Rodríguez-Rodríguez; María Ángeles Martín-Cabrejas; Vanesa Benítez; Ángel Luis López de Pablo; María Del Carmen González; Silvia M. Arribas. Male fetal sex is associated with low maternal plasma anti-inflammatory cytokine profile in the first trimester of healthy pregnancies. Cytokine 2020, 136, 155290 .
AMA StyleDavid Ramiro-Cortijo, María de la Calle, Rainer Böger, Juliane Hannemann, Nicole Lüneburg, María Rosario López-Giménez, Pilar Rodríguez-Rodríguez, María Ángeles Martín-Cabrejas, Vanesa Benítez, Ángel Luis López de Pablo, María Del Carmen González, Silvia M. Arribas. Male fetal sex is associated with low maternal plasma anti-inflammatory cytokine profile in the first trimester of healthy pregnancies. Cytokine. 2020; 136 ():155290.
Chicago/Turabian StyleDavid Ramiro-Cortijo; María de la Calle; Rainer Böger; Juliane Hannemann; Nicole Lüneburg; María Rosario López-Giménez; Pilar Rodríguez-Rodríguez; María Ángeles Martín-Cabrejas; Vanesa Benítez; Ángel Luis López de Pablo; María Del Carmen González; Silvia M. Arribas. 2020. "Male fetal sex is associated with low maternal plasma anti-inflammatory cytokine profile in the first trimester of healthy pregnancies." Cytokine 136, no. : 155290.
Breast milk (BM) is beneficial due to its content in a wide range of different antioxidants, particularly relevant for preterm infants, who are at higher risk of oxidative stress. We hypothesize that BM antioxidants are adapted to gestational age and are negatively influenced by maternal age. Fifty breastfeeding women from two hospitals (Madrid, Spain) provided BM samples at days 7, 14 and 28 of lactation to assess total antioxidant capacity (ABTS), thiol groups, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase activities, lipid peroxidation (malondialdehyde, MDA + 4-Hydroxy-Trans-2-Nonenal, HNE), protein oxidation (carbonyl groups) (spectrophotometry) and melatonin (ELISA). Mixed random-effects linear regression models were used to study the influence of maternal and gestational ages on BM antioxidants, adjusted by days of lactation. Regression models evidenced a negative association between maternal age and BM melatonin levels (β = −7.4 ± 2.5; p-value = 0.005); and a negative association between gestational age and BM total antioxidant capacity (β = −0.008 ± 0.003; p-value = 0.006), SOD activity (β = −0.002 ± 0.001; p-value = 0.043) and protein oxidation (β = −0.22 ± 0.07; p-value = 0.001). In conclusion, BM antioxidants are adapted to gestational age providing higher levels to infants with lower degree of maturation; maternal ageing has a negative influence on melatonin, a key antioxidant hormone.
Andrea Gila-Díaz; Gloria Herranz Carrillo; Silvia Cañas; Miguel Saenz De Pipaón; José Antonio Martínez-Orgado; Pilar Rodríguez-Rodríguez; Ángel Luis López De Pablo; María A. Martin-Cabrejas; David Ramiro-Cortijo; Silvia M. Arribas. Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation. Nutrients 2020, 12, 2569 .
AMA StyleAndrea Gila-Díaz, Gloria Herranz Carrillo, Silvia Cañas, Miguel Saenz De Pipaón, José Antonio Martínez-Orgado, Pilar Rodríguez-Rodríguez, Ángel Luis López De Pablo, María A. Martin-Cabrejas, David Ramiro-Cortijo, Silvia M. Arribas. Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation. Nutrients. 2020; 12 (9):2569.
Chicago/Turabian StyleAndrea Gila-Díaz; Gloria Herranz Carrillo; Silvia Cañas; Miguel Saenz De Pipaón; José Antonio Martínez-Orgado; Pilar Rodríguez-Rodríguez; Ángel Luis López De Pablo; María A. Martin-Cabrejas; David Ramiro-Cortijo; Silvia M. Arribas. 2020. "Influence of Maternal Age and Gestational Age on Breast Milk Antioxidants During the First Month of Lactation." Nutrients 12, no. 9: 2569.
We aimed to design and validate a new questionnaire of adherence to healthy food pyramid (HFP) (AP-Q), to improve previous instruments. The questionnaire was self-administered and included 28 questions from 10 categories (physical activity, health habits, hydration, grains, fruits, vegetables, oil type, dairy products, animal proteins, and snacks). A population of 130 Spanish adults answered it, obtaining scores from each category and a global score of HFP adherence (AP-Q score). Validation was performed through principal components analysis (PCA) and internal consistency by Cronbach’s alpha. AP-Q was also externally validated with Kidmed-test, answered by 45 individuals from the cohort. The global AP-Q score was 5.1 ± 1.3, with an internal consistency of 64%. The PCA analysis extracted seven principal components, which explained 68.5% of the variance. The global AP-Q score was positively associated with Kidmed-test score. Our data suggest that AP-Q is a complete and robust questionnaire to assess HFP adherence, with several advantages: easy to complete, cost-effective, timesaving and has the competency to assess, besides diet, several features affecting health status, lacking in other instruments. We suggest that AP-Q could be useful in epidemiological research, although it requires additional calibration to analyze its reproducibility and validation in other populations.
Andrea Gila-Díaz; Silvia M. Arribas; Ángel Luis López De Pablo; Ma Rosario López-Giménez; Sophida Phuthong; David Ramiro-Cortijo. Development and Validation of a Questionnaire to Assess Adherence to the Healthy Food Pyramid in Spanish Adults. Nutrients 2020, 12, 1656 .
AMA StyleAndrea Gila-Díaz, Silvia M. Arribas, Ángel Luis López De Pablo, Ma Rosario López-Giménez, Sophida Phuthong, David Ramiro-Cortijo. Development and Validation of a Questionnaire to Assess Adherence to the Healthy Food Pyramid in Spanish Adults. Nutrients. 2020; 12 (6):1656.
Chicago/Turabian StyleAndrea Gila-Díaz; Silvia M. Arribas; Ángel Luis López De Pablo; Ma Rosario López-Giménez; Sophida Phuthong; David Ramiro-Cortijo. 2020. "Development and Validation of a Questionnaire to Assess Adherence to the Healthy Food Pyramid in Spanish Adults." Nutrients 12, no. 6: 1656.
Twin pregnancies are increasing due to the rise in mothers’ childbearing age and have a higher risk of fetal growth restriction (FGR) and prematurity. Therefore, early prediction of these events is important. Our aim was to analyze in the first trimester of pregnancy a possible association between antioxidants, including melatonin, in maternal plasma and the development of fetal complications in twin pregnancies. A single-center, prospective, and observational study was performed in 104 twin-pregnant women. A blood sample was extracted between the 9th and the 11th week of gestation, and plasma was obtained. Antioxidants (thiols, reduced glutathione, phenolic compounds, catalase, superoxide dismutase) and oxidative damage biomarkers (carbonyl groups and malondialdehyde) were assessed by spectrophotometry, and global scores were calculated from these parameters (Antiox-S, Prooxy-S). Melatonin and cortisol were evaluated by a competitive immunoassay. In the first trimester of pregnancy, Antiox-S was significantly lower in women who developed FGR compared to those with normal fetal growth; plasma melatonin was significantly lower in women with preterm compared to those with full-term births and exhibited a positive correlation with birth weight. Maternal cortisol showed a negative correlation with birth weight. We conclude that, for twin gestations, maternal plasma antioxidant status and melatonin could be potential biomarkers to be included in algorithms to predict FGR and preterm labor.
David Ramiro-Cortijo; María De La Calle; Pilar Rodríguez-Rodríguez; Ángel L. López De Pablo; María R. López-Giménez; Yolanda Aguilera; María A. Martín-Cabrejas; María Del Carmen González; Silvia M. Arribas. Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study. Antioxidants 2020, 9, 269 .
AMA StyleDavid Ramiro-Cortijo, María De La Calle, Pilar Rodríguez-Rodríguez, Ángel L. López De Pablo, María R. López-Giménez, Yolanda Aguilera, María A. Martín-Cabrejas, María Del Carmen González, Silvia M. Arribas. Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study. Antioxidants. 2020; 9 (4):269.
Chicago/Turabian StyleDavid Ramiro-Cortijo; María De La Calle; Pilar Rodríguez-Rodríguez; Ángel L. López De Pablo; María R. López-Giménez; Yolanda Aguilera; María A. Martín-Cabrejas; María Del Carmen González; Silvia M. Arribas. 2020. "Maternal Antioxidant Status in Early Pregnancy and Development of Fetal Complications in Twin Pregnancies: A Pilot Study." Antioxidants 9, no. 4: 269.
Melatonin is a multifunctional antioxidant neurohormone found in plant foods such as lentil sprouts. We aim to evaluate the effect of lentil sprout intake on the plasmatic levels of melatonin and metabolically related compounds (plasmatic serotonin and urinary 6-sulfatoxymelatonin), total phenolic compounds, and plasmatic antioxidant status, and compare it with synthetic melatonin. The germination of lentils increases the content of melatonin. However, the phenolic content diminished due to the loss of phenolic acids and flavan-3-ols. The flavonol content remained unaltered, being the main phenolic family in lentil sprouts, primarily composed of kaempferol glycosides. Sprague Dawley rats were used to investigate the pharmacokinetic profile of melatonin after oral administration of a lentil sprout extract and to evaluate plasma and urine melatonin and related biomarkers and antioxidant capacity. Melatonin showed maximum concentration (45.4 pg/mL) 90 min after lentil sprout administration. The plasmatic melatonin levels increased after lentil sprout intake (70%, p < 0.05) with respect to the control, 1.2-fold more than after synthetic melatonin ingestion. These increments correlated with urinary 6-sulfatoxymelatonin content (p < 0.05), a key biomarker of plasmatic melatonin. Nonetheless, the phenolic compound content did not exhibit any significant variation. Plasmatic antioxidant status increased in the antioxidant capacity upon both lentil sprout and synthetic melatonin administration. For the first time, we investigated the bioavailability of melatonin from lentil sprouts and its role in plasmatic antioxidant status. We concluded that their intake could increase melatonin plasmatic concentration and attenuate plasmatic oxidative stress.
Miguel Rebollo-Hernanz; Yolanda Aguilera; Teresa Herrera; L. Tábata Cayuelas; Montserrat Dueñas; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Silvia M. Arribas; María A. Martín-Cabrejas. Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats. Foods 2020, 9, 330 .
AMA StyleMiguel Rebollo-Hernanz, Yolanda Aguilera, Teresa Herrera, L. Tábata Cayuelas, Montserrat Dueñas, Pilar Rodríguez-Rodríguez, David Ramiro-Cortijo, Silvia M. Arribas, María A. Martín-Cabrejas. Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats. Foods. 2020; 9 (3):330.
Chicago/Turabian StyleMiguel Rebollo-Hernanz; Yolanda Aguilera; Teresa Herrera; L. Tábata Cayuelas; Montserrat Dueñas; Pilar Rodríguez-Rodríguez; David Ramiro-Cortijo; Silvia M. Arribas; María A. Martín-Cabrejas. 2020. "Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats." Foods 9, no. 3: 330.
Human breast milk is the optimal source of nutrition for infant growth and development. Breast milk fats and their downstream derivatives of fatty acids and fatty acid-derived terminal mediators not only provide an energy source but also are important regulators of development, immune function, and metabolism. The composition of the lipids and fatty acids determines the nutritional and physicochemical properties of human milk fat. Essential fatty acids, including long-chain polyunsaturated fatty acids (LCPUFAs) and specialized pro-resolving mediators, are critical for growth, organogenesis, and regulation of inflammation. Combined data including in vitro, in vivo, and human cohort studies support the beneficial effects of human breast milk in intestinal development and in reducing the risk of intestinal injury. Human milk has been shown to reduce the occurrence of necrotizing enterocolitis (NEC), a common gastrointestinal disease in preterm infants. Preterm infants fed human breast milk are less likely to develop NEC compared to preterm infants receiving infant formula. Intestinal development and its physiological functions are highly adaptive to changes in nutritional status influencing the susceptibility towards intestinal injury in response to pathological challenges. In this review, we focus on lipids and fatty acids present in breast milk and their impact on neonatal gut development and the risk of disease.
David Ramiro-Cortijo; Pratibha Singh; Yan Liu; Esli Medina-Morales; William Yakah; Steven D. Freedman; Camilia R. Martin. Breast Milk Lipids and Fatty Acids in Regulating Neonatal Intestinal Development and Protecting against Intestinal Injury. Nutrients 2020, 12, 534 .
AMA StyleDavid Ramiro-Cortijo, Pratibha Singh, Yan Liu, Esli Medina-Morales, William Yakah, Steven D. Freedman, Camilia R. Martin. Breast Milk Lipids and Fatty Acids in Regulating Neonatal Intestinal Development and Protecting against Intestinal Injury. Nutrients. 2020; 12 (2):534.
Chicago/Turabian StyleDavid Ramiro-Cortijo; Pratibha Singh; Yan Liu; Esli Medina-Morales; William Yakah; Steven D. Freedman; Camilia R. Martin. 2020. "Breast Milk Lipids and Fatty Acids in Regulating Neonatal Intestinal Development and Protecting against Intestinal Injury." Nutrients 12, no. 2: 534.
Prematurity and enteral feedings are major risk factors for intestinal injury leading to necrotizing enterocolitis (NEC). An immature digestive system can lead to maldigestion of macronutrients and increased vulnerability to intestinal injury. The aim of this study was to test in neonatal mice the effect of maltodextrin, a complex carbohydrate, on the risk of intestinal injury. The goal was to develop a robust and highly reproducible murine model of intestinal injury that allows insights into the pathogenesis and therapeutic interventions of nutrient driven intestinal injury. Five to 6-day old C57BL/6 mice were assigned to the following groups: dam fed (D); D+hypoxia+Klebsiella pneumoniae; maltodextrin-dominant human infant formula (M) only; M+hypoxia; and M+hypoxia+Klebsiella pneumoniae. The mice in all M groups were gavage fed five times a day for 4 days. Mice were exposed to hypoxia twice a day for 10 min prior to the first and last feedings and Klebsiella pneumoniae was added to feedings per group assignment. Mice in all M groups demonstrated reduced body weight, increased small intestinal dilatation, and increased intestinal injury scores. Maltodextrin-dominant infant formula with hypoxia led to intestinal injury in neonatal mice accompanied by loss of villi, increased MUC2 production, altered expression of tight junction proteins, enhanced intestinal permeability, increased cell death, and higher levels of intestinal inflammatory mediators. This robust and highly reproducible model allows for further interrogation of the role of nutrients on pathogenic factors leading to intestinal injury and NEC in preterm infants.
Pratibha Singh; Lady Leidy Sanchez-Fernandez; David Ramiro-Cortijo; Pedro Ochoa-Allemant; George Perides; Yan Liu; Esli Medina-Morales; William Yakah; Steven D. Freedman; Camilia R. Martin. Maltodextrin-induced intestinal injury in a neonatal mouse model. Disease Models & Mechanisms 2020, 13, 1 .
AMA StylePratibha Singh, Lady Leidy Sanchez-Fernandez, David Ramiro-Cortijo, Pedro Ochoa-Allemant, George Perides, Yan Liu, Esli Medina-Morales, William Yakah, Steven D. Freedman, Camilia R. Martin. Maltodextrin-induced intestinal injury in a neonatal mouse model. Disease Models & Mechanisms. 2020; 13 (8):1.
Chicago/Turabian StylePratibha Singh; Lady Leidy Sanchez-Fernandez; David Ramiro-Cortijo; Pedro Ochoa-Allemant; George Perides; Yan Liu; Esli Medina-Morales; William Yakah; Steven D. Freedman; Camilia R. Martin. 2020. "Maltodextrin-induced intestinal injury in a neonatal mouse model." Disease Models & Mechanisms 13, no. 8: 1.
After birth, preterm infants are deficient in arachidonic acid (ARA), docosahexaenoic acid (DHA), and antioxidants, increasing their risk of oxidative stress-related pathologies. The principal aim was to evaluate if supplementation with long-chain polyunsaturated fatty acids (LCPUFAs) improves antioxidant defenses. In total, 21 preterm infants were supplemented with ARA and DHA in a 2:1 ratio (ARA:DHA-S) or with medium-chain triglycerides (MCT-S). Plasma n-3 and n-6 LCPUFAs were measured at birth, postnatal day 28, and 36 weeks of postmenstrual age (36 WPA) by gas chromatography–mass spectroscopy. Plasma antioxidants (glutathione (GSH), catalase, and thiols) and oxidative damage biomarkers (malondialdehyde (MDA), carbonyls) were analyzed at the same time points by spectrophotometry, and scores of antioxidant status (Antiox-S) and oxidative damage (Proxy-S) were calculated. At 36 WPA, linoleic acid (LA) and dihomo-γ-linolenic acid (DGLA) were decreased in ARA:DHA-S compared to the MCT-S group (LA: ARA:DHA-S = 18.54 ± 1.68, MCT-S = 22.80 ± 1.41; p = 0.018; DGLA: ARA:DHA-S = 1.68 ± 0.38, MCT-S = 2.32 ± 0.58; p = 0.018). Furthermore, α-linolenic acid (ALA) was increased in ARA:DHA-S (ARA:DHA-S = 0.52 ± 0.33, MCT-S = 0.22 ± 0.10; p = 0.018). Additionally, LA:DHA ratio was decreased in the ARA:DHA-S compared to control group (ARA:DHA-S = 6.26 ± 2.35, MCT-S = 8.21 ± 2.65; p = 0.045). By the end of supplementation (36 WPA), catalase, thiol groups, and Antiox-S were significantly higher in neonates receiving ARA:DHA-S compared to those receiving MCT-S, with no differences in oxidative stress biomarkers. In conclusion, ARA:DHA supplementation in preterm neonates resulted in an overall improvement in antioxidant to oxidant balance and a decrease in early fatty acid precursors of the n-6 relative to the n-3 pathway. These effects may reduce oxidative stress and inflammation.
David Ramiro-Cortijo; Ángel Luis López De Pablo; Ma Rosario López-Giménez; Camilia R. Martin; Joanne Brown; Miguel Saenz De Pipaón; Silvia M. Arribas. Plasma Oxidative Status in Preterm Infants Receiving LCPUFA Supplementation: A Pilot Study. Nutrients 2020, 12, 122 .
AMA StyleDavid Ramiro-Cortijo, Ángel Luis López De Pablo, Ma Rosario López-Giménez, Camilia R. Martin, Joanne Brown, Miguel Saenz De Pipaón, Silvia M. Arribas. Plasma Oxidative Status in Preterm Infants Receiving LCPUFA Supplementation: A Pilot Study. Nutrients. 2020; 12 (1):122.
Chicago/Turabian StyleDavid Ramiro-Cortijo; Ángel Luis López De Pablo; Ma Rosario López-Giménez; Camilia R. Martin; Joanne Brown; Miguel Saenz De Pipaón; Silvia M. Arribas. 2020. "Plasma Oxidative Status in Preterm Infants Receiving LCPUFA Supplementation: A Pilot Study." Nutrients 12, no. 1: 122.
Preterm birth is an increasing worldwide problem. Prematurity is the second most common cause of death in children under 5 years of age. It is associated with a higher risk of several pathologies in the perinatal period and adulthood. Maternal milk, a complex fluid with several bioactive factors, is the best option for the newborn. Its dynamic composition is influenced by diverse factors such as maternal age, lactation period, and health status. The aim of the present review is to summarize the current knowledge regarding some bioactive factors present in breastmilk, namely antioxidants, growth factors, adipokines, and cytokines, paying specific attention to prematurity. The revised literature reveals that the highest levels of these bioactive factors are found in the colostrum and they decrease along the lactation period; bioactive factors are found in higher levels in preterm as compared to full-term milk, they are lacking in formula milk, and decreased in donated milk. However, there are still some gaps and inconclusive data, and further research in this field is needed. Given the fact that many preterm mothers are unable to complete breastfeeding, new information could be important to develop infant supplements that best match preterm human milk.
Andrea Gila-Diaz; Silvia M. Arribas; Alba Algara; María A. Martín-Cabrejas; Ángel Luis López De Pablo; Miguel Sáenz De Pipaón; David Ramiro-Cortijo. A Review of Bioactive Factors in Human Breastmilk: A Focus on Prematurity. Nutrients 2019, 11, 1307 .
AMA StyleAndrea Gila-Diaz, Silvia M. Arribas, Alba Algara, María A. Martín-Cabrejas, Ángel Luis López De Pablo, Miguel Sáenz De Pipaón, David Ramiro-Cortijo. A Review of Bioactive Factors in Human Breastmilk: A Focus on Prematurity. Nutrients. 2019; 11 (6):1307.
Chicago/Turabian StyleAndrea Gila-Diaz; Silvia M. Arribas; Alba Algara; María A. Martín-Cabrejas; Ángel Luis López De Pablo; Miguel Sáenz De Pipaón; David Ramiro-Cortijo. 2019. "A Review of Bioactive Factors in Human Breastmilk: A Focus on Prematurity." Nutrients 11, no. 6: 1307.
Bariatric surgery (BS) results in sustained weight loss and may reverse inflammation, metabolic alterations, extracellular matrix remodeling and arterial stiffness. We hypothesize that increased stiffening in omental arteries from obese patients might be associated with an increase in MMP activity and a decrease in p-AMPK, together with systemic oxidative stress and inflammation. Moreover, BS could contribute to reversing these alterations. This study was conducted with 38 patients of Caucasian origin: 31 adult patients with morbid obesity (9 men and 22 women; mean age 46 years and BMI = 42.7 ± 1.0 kg/m2) and 7 non-obese subjects (7 women; mean age 45 years and BMI = 22.7 ± 0.6 kg/m2). Seventeen obese patients were studied before and 12 months after BS. The stiffness index β, an index of intrinsic arterial stiffness, was determined in omental arteries and was significantly higher in obese patients. Levels of phosphorylated AMPK (p-AMPKThr-172) and SIRT-1 were significantly lower in peripheral blood mononuclear cells (PBMCs) from obese patients than those from non-obese patients (p < 0.05) and were normalized after BS. Total and active MMP-9 activities, LDH, protein carbonyls and uric acid were higher in obese patients and reduced by BS. Moreover, there was a correlation between plasmatic LDH levels and the stiffness index β. BS has a beneficial effect on abnormal MMP-9, LDH and AMPK activities that might be associated with the development of arterial stiffness in obese patients. Since these parameters are easily measured in blood samples, they could constitute potential biomarkers of cardiovascular risk in morbid obesity.
Concha F. García-Prieto; Marta Gil-Ortega; Elena Vega-Martín; David Ramiro-Cortijo; Miriam Martín-Ramos; Elena Bordiú; Andrés Sanchez-Pernaute; Antonio Torres; I. Aránguez; Maria S. Fernandez-Alfonso; Miguel A. Rubio; Beatriz Somoza. Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk. Frontiers in Physiology 2019, 10, 1 .
AMA StyleConcha F. García-Prieto, Marta Gil-Ortega, Elena Vega-Martín, David Ramiro-Cortijo, Miriam Martín-Ramos, Elena Bordiú, Andrés Sanchez-Pernaute, Antonio Torres, I. Aránguez, Maria S. Fernandez-Alfonso, Miguel A. Rubio, Beatriz Somoza. Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk. Frontiers in Physiology. 2019; 10 ():1.
Chicago/Turabian StyleConcha F. García-Prieto; Marta Gil-Ortega; Elena Vega-Martín; David Ramiro-Cortijo; Miriam Martín-Ramos; Elena Bordiú; Andrés Sanchez-Pernaute; Antonio Torres; I. Aránguez; Maria S. Fernandez-Alfonso; Miguel A. Rubio; Beatriz Somoza. 2019. "Beneficial Effect of Bariatric Surgery on Abnormal MMP-9 and AMPK Activities: Potential Markers of Obesity-Related CV Risk." Frontiers in Physiology 10, no. : 1.
Arachidonic and docosahexaenoic acids (ARA and DHA) are important during pregnancy. However, the effects of dietary supplementation on fetal growth and oxidative stress are inconclusive. We aimed to assess the effect of high ARA and DHA diet during rat gestation on: (1) ARA and DHA availability in plasma and placenta, (2) fetal growth, and (3) placental oxidative stress, analyzing the influence of sex. Experimental diet (ED) was prepared by substituting soybean oil in the control diet (CD) by a fungi/algae-based oil containing ARA and DHA (2:1). Rats were fed with CD or ED during gestation; plasma, placenta, and fetuses were obtained at gestational day 20. DHA, ARA, and their precursors were analyzed in maternal plasma and placenta by gas chromatography/mass spectrophotometry. Fetuses and placentas were weighed, the proportion of fetuses with intrauterine growth restriction (IUGR) determined, and placental lipid and protein oxidation analyzed. ED fetuses exhibited lower body weight compared to CD, being >40% IUGR; fetal weight negatively correlated with maternal plasma ARA, but not DHA. Only ED female placenta exhibited higher lipid and protein oxidation compared to its CD counterparts; lipid peroxidation is negatively associated with fetal weight. In conclusion, high ARA during gestation associates with IUGR, through placental oxidative stress, with females being more susceptible.
Cynthia Guadalupe Reyes-Hernández; David Ramiro-Cortijo; Pilar Rodríguez-Rodríguez; Sonia Giambelluca; Manuela Simonato; M Carmen Gonzalez-Garcia; Angel Luis López De Pablo; Mª Del Rosario López-Giménez; Paola Cogo; Miguel Sáenz De Pipaón; Virgilio P. Carnielli; Silvia M. Arribas; Angel López De Pablo. Effects of Arachidonic and Docosohexahenoic Acid Supplementation during Gestation in Rats. Implication of Placental Oxidative Stress. International Journal of Molecular Sciences 2018, 19, 3863 .
AMA StyleCynthia Guadalupe Reyes-Hernández, David Ramiro-Cortijo, Pilar Rodríguez-Rodríguez, Sonia Giambelluca, Manuela Simonato, M Carmen Gonzalez-Garcia, Angel Luis López De Pablo, Mª Del Rosario López-Giménez, Paola Cogo, Miguel Sáenz De Pipaón, Virgilio P. Carnielli, Silvia M. Arribas, Angel López De Pablo. Effects of Arachidonic and Docosohexahenoic Acid Supplementation during Gestation in Rats. Implication of Placental Oxidative Stress. International Journal of Molecular Sciences. 2018; 19 (12):3863.
Chicago/Turabian StyleCynthia Guadalupe Reyes-Hernández; David Ramiro-Cortijo; Pilar Rodríguez-Rodríguez; Sonia Giambelluca; Manuela Simonato; M Carmen Gonzalez-Garcia; Angel Luis López De Pablo; Mª Del Rosario López-Giménez; Paola Cogo; Miguel Sáenz De Pipaón; Virgilio P. Carnielli; Silvia M. Arribas; Angel López De Pablo. 2018. "Effects of Arachidonic and Docosohexahenoic Acid Supplementation during Gestation in Rats. Implication of Placental Oxidative Stress." International Journal of Molecular Sciences 19, no. 12: 3863.