To solve the problem of dye contamination caused by methylene blue (MB), a one-step synthesised nanocellulose (CNF) and polyacrylamide (PAM) gel network was modified by using NaOH in this study, and the prepared samples were analysed for their micromorphology, chemical structure, and adsorption-release properties. The findings demonstrated that the maximum adsorption capacity of the CNF-PAM5% was 172.08 mg/g, which followed the quasi-second-order kinetic model and the Freundlich adsorption model. The adsorption of the gel increased with the increase of the NaOH-modified concentration. However, the adsorption efficiency of the CNF-PAM5% could still reach 85% after four cycles, and the CNF-PAM5% remained intact without signs of fragmentation after 4 h of stirring and water impact, which was attributed to the introduction of CNF into the PAM network to effectively improve the mechanical properties of the gel. Moreover, toxicity tests showed no significant difference in the amount of cellular activity, even when the volume of the CNF-PAM5% sample was increased up to 10-fold. This gel, which exhibits low toxicity and excellent recycling properties, serves to reduce environmental impact during the adsorption process. Furthermore, the potential exists for utilising the gel’s methylene blue-releasing (MB) properties as a fungicide for fish.

The application of hydrogels in flexible sensing has received increasing attention, but the simultaneous preparation of hydrogels with good structural stability, strain sensing sensitivity, freezing resistance, and drying resistance remains a challenge. Based on this, a GG-nanocellulose/sodium alginate/polyacrylamide composite hydrogel with a hierarchical network structure was constructed by one-step synthesis by incorporating graphene oxide (GO) and glycerol into the hydrogel. The hydrogel remained structurally intact after 100 compression cycles. In addition, the hydrogel was dried at 30 °C for 24 h. The mass retention rate was 48%, the melting peak was as low as −13.87 °C, and the hydrogel remained flexible and stable at low temperatures. GO modulated the network structure arrangement of the hydrogel through various mechanisms, thereby conferring to the hydrogel an excellent sensing performance, with a sensitivity (GF) of 2.21. In conclusion, this hierarchical network hydrogel has good drying, freezing, and sensing properties, which provides a new viable strategy for monitoring motion signals. Moreover, the hydrogel is predicted to function as a dressing, thereby facilitating the absorption of heat from the skin’s surface, with the aim of alleviating the discomfort associated with joint and muscle injuries caused by strenuous exercise.

As one of the decay-resistant woods, Korean pine is widely used in the construction industry. However, even the most corrosion-resistant wood is still susceptible to decay under the right humidity and temperature conditions. In this study, Bacillus amyloliquefaciens (B. amyloliquefaciens) bacterial liquid and filter bacterial solution were prepared for the anti-corrosion treatment of Korean pine wood, aiming to improve its decay-resistant property. Through the plate confrontation test, it was discovered that B. amyloliquefaciens AW3 could significantly inhibit the growth of Fomitopsis pinicola (F. Pinicola). The results of mass loss rate, mechanical properties test, XRD, FTIR and SEM analysis showed that the preserved Korean pine wood had significant improvement in various properties compared with the decayed wood, which was manifested in the significant reduction of mass loss, improvement of mechanical properties, and increased wood cellulose diffraction peak intensity. There is no mycelium infection of F. pinicola in Korean pine wood, and the antiseptic liquid can penetrate into the wood evenly, which plays an effective antiseptic role. The B. amyloliquefaciens bacterial liquid exhibited superior preservative performance compared to the B. amyloliquefaciens filter bacterial solution. In conclusion, B. amyloliquefaciens, as an efficient and environmentally friendly biological preservative, holds broad application prospects in improving the anti-corrosion performance of Korean pine wood.

In this study, whole-genome resequencing was performed on the cold-tolerant variety ND-6 and the cold-sensitive variety ‘Sorbonne’. After evaluation, the Lilium davidii var. unicolor reference genome was selected to analyze SSR distribution characteristics. Whole-genome InDel identification and comparative analysis were conducted for the two varieties, yielding 34,812,909 and 24,497,857 InDels, respectively. Short InDels were predominant, with deletions slightly outnumbering insertions, mostly located in intergenic regions. Twenty pairs of SSR primers were screened and synthesized. Among them, 10 pairs amplified clearly, with a polymorphism rate of 82.6%, effectively distinguishing the two cultivars examined in this study. This study provides systematic data and a reliable marker resource for the analysis of lily genomic variation, laying a foundation for the identification of cold-tolerant germplasm; validation across additional cultivars and individuals will be required to extend their utility to broader germplasm.