Ethanol and acetone extracts of Cucurbita pepo leaves were in vitro analyzed to determine the vitamin contents, antioxidant, anticancer, and antimicrobial activity of the leaves. According to HPLC analyses, the leaves contained slight amounts of vitamin C, A, and E. The analysis for antioxidant activity revealed that both ethanol and acetone extracts exhibited high activity values against ABTS+•, OH•, and DPPH• radicals. The total phenols and flavonoid assay revealed high values for the extracts. The MTT test revealed that both ethanol and acetone extracts exhibited cytotoxic activity toward human breast cancer cells (MCF-7). But no significant activities observed against hepatocellular carcinoma cells (HepG-2). The antimicrobial activity assay revealed activity of the extracts towards selected Gram's positive and Gram's negative bacteria. These results have provided scientific motivation to use Cucurbita pepo leaves as a medicinal plant to treat oxidative and inflammatory-related diseases.

2,2'-[(2R,3R)-2,3-dihydroxy-1,4-dioxobutane-1,4-diyl]bis(N-aryl-alkylhydrazine-1-carbothioamide (T1–5) were obtained by the interaction of (2R,3R)-2,3-dihydroxybutanedihydrazide (1) with five different isothiocyanate alkyl(aryl) derivatives. The structures of the final compounds were confirmed by elemental analyses, FT-IR, ¹H-NMR and ¹³C-NMR. The antioxidant, antimicrobial and anticancer properties of the synthesized compounds were also investigated. Three of the triazole derivatives with p-tolyl, benzyl and phenyl substituents (T3–5) displayed good antioxidant and anticancer activity in comparison to the standards.

Three-dimensional (3D)-printed permanent crown resins are increasingly used in restorative dentistry, but their residual monomer elution compared with computer-aided design/computer-aided manufacturing (CAD/CAM) materials remains insufficiently characterized. This in vitro study compared residual monomer elution from three permanent crown materials at different storage times. A CAD/CAM hybrid ceramic (GC Cerasmart 270) and two 3D-printed permanent crown resins (Graphy Tera Harz and Saremco CrownTec) were evaluated. Specimens (12 × 14 × 2 mm; n = 5 per material) were stored in 75% ethanol, and the extraction solutions were analyzed by high-performance liquid chromatography (HPLC) on days 1 and 30. The eluted amounts of Bis-GMA, TEGDMA, UDMA, and HEMA were quantified and analyzed using repeated-measures ANOVA, Kruskal–Wallis, and Wilcoxon signed-rank tests (p < 0.05). Material, time, and their interaction were significant for Bis-GMA (p < 0.001). Material differences were also significant for TEGDMA and UDMA (p = 0.002), whereas the time-dependent increases in TEGDMA, UDMA, and HEMA did not reach statistical significance. Graphy showed the highest Bis-GMA and UDMA elution, Saremco the highest TEGDMA and HEMA elution, and Cerasmart the lowest values for all monomers. Both material characteristics and storage time should therefore be considered when evaluating residual monomer elution.

Purpose: The in vivo and in vitro antitumor activities of hydroxyurea derivative Schiff bases (SBs) metalcomplexes were investigated in immortalized human colon cancer cell lines (HT-29 cells) and ratmodels.Methods: For the in vitro studies, three concentrations (5, 10, and 20 μM) of the 1-hydroxy-3-(E)-pyridine-3-ylmethylidene urea derivative SB (L)-metal complexes (L-Cd, L-Cu, L-Zn) were used todetermine the viability of HT-29 cell line with dimethylsulphoxide (DMSO) as control. On the other hand,colorectal cancer was induced with subcutaneous administration of azoxymethane (AOM; 15 mg/kg) in35 Wistar albino rats, which were assigned to five treatment groups consisting of DMSO (negativecontrol), cisplatin (15 mg/kg, positive control), AOM + L-Cd (25 mg/kg), AOM + L-Cu (25 mg/kg) andAOM + L-Zn (25 mg/kg) groups, respectively. Tumor formation was observed by macroscopic andmicroscopic examinations.Results: Tumour formation was not observed in the positive control group. The rats treated with AOM(excluding L-Cu and cisplatin group) displayed severe dysplasia and adenocarcinoma formations in theoil+DMSO, L-Cd and L-Zn groups. When compared with the cisplatin group in the in vivo studies, the Cucomplex had a more favorable effect against colon cancer.Conclusion: Consequently, hydroxyurea derivative SB-metal complexes exhibit antiproliferative activityin both in vitro (p < 0.0001) and in vivo studies.