This research aimed to determine the effectiveness of several types of liquid organic fertilizer (LOF) derived from plant waste and the dose of P fertilizer on the growth and yield of upland rice on degraded acid soil. The treatments consisted of two factors. The first factor (A) was several types of LOF consisting of A1 (control), A2 (LOF containing isolates of P-solubilizing bacteria), A3 (LOF-banana waste), and A4 (A2+A3). The second factor (B) was fertilizer doses consisting of B1 (50% of the recommended dose of P fertilizer), B2 (75% of the recommended dose of P fertilizer), and B3 (100% of the recommended dose of P fertilizer). The treatment combinations were arranged in a factorial randomized block design with three replications. The research results showed that applying either LOF-banana waste or the mixture of LOF-banana waste and LOF-containing isolates of P-solubilizing bacteria improved plant height, the number of tillers, and N and P uptake by the plant. The rice grain yields among the treatments were not significantly different, but the highest rice grain yield (139.8 g/pot) was obtained at the treatment of the mixture of LOF-P isolate + LOF-banana waste (A2+A3 treatments). The 75% of the recommended dose of P fertilizer was not significantly different from 100% P fertilizer with LOF on growth and yield upland rice and nutrient uptake by the plant.

This study aimed to evaluate the ability of microbes from the rumen contents of cattle as antagonists of pathogenic fungi and as liquid organic fertilizer (LOF) containing P-solubilizing bacteria in rice plants. The mixture consisting of 5 kg rumen contents of cattle, 2 kg of rice bran, 1 kg of molasses, and 10 L of tap water was fermented for 14 days. After fermentation, the mixture was filtered to separate the liquid from the solid components. The solid portion was collected for the isolation and screening of contained bacteria. Subsequent tests included biochemical tests, antagonistic activity tests, DNA extraction, PCR amplification, phosphate solubilization ability testing, inhibitory ability study, and application of LOF in a screen house. One bacterial isolate (PTK2TB code) exhibited the highest antagonistic activity against three tested pathogenic fungi (inhibits more than 50%). Subsequent 16SrDNA sequence analysis showed that this isolate belongs to the same group as the type strain and reference strain of Bacillus amyloliquefaciens. Furthermore, out of the 90 bacterial isolates obtained, 20 demonstrated a high capacity for phosphate solubilization. Additionally, LOF containing phosphate-solubilizing bacterial isolates positively impacted rice growth and yield.