Objectives: Mesenchymal stem cells are self-renewing stem cells. The human foreskin has potential to be used as a source of stem cells. The aim of the study was to obtain spheroid formation of human foreskin cells (hnFSSCs) isolated from newborn human foreskin tissue. In addition, the apoptotic and proliferative effects of a traditional plant, Corchorus olitorius L. (C. olitorius), on hnFSSC spheroids were investigated. Materials and Methods: After a routine circumcision procedure the cells were isolated and cultured in suitable medium. The plant leaves was extracted with ethanol and their composition was analyzed by liquid chromatography coupled with mass spectrometry (LC-MS/MS). The foreskin stem cells were characterized immunocytochemically by CD45, CD34, and CD90 antibodies. hnFSSC spheroids were formed using the hanging drop technique. Immunofluorescence staining was used on the obtained spheroids to determine the distribution of caspase-3 and Ki-67 after being treated with C. olitorius extract for 48 h. Results: Immunostaining analysis showed that hnFSSCs were positive for CD45 and CD34 and negative for CD90. According to LC-MS/MS C. olitorius was rich in flavanols and hydrocinnamic acid derivatives. Although the spheroids obtained were loose and floating, the cells interacted with each other. Caspase-3 activity was higher in the control group than in the extract-treated group and Ki-67 was higher in the extract-treated group than in the control group, suggesting that the plant might have the capacity to increase stem cell proliferation due to its rich polyphenolic content. Conclusion: The results suggest that hnFSSCs and spheroids might be used in stem cell generation, tissue repair and renewal as human foreskin tissue has potential to be used as a stem cell source. C. olitorius also increased proliferation of hnFSSCs, showing that polyphenols might increase proliferation of stem cells.

Giriş Hülya YÜCEL Rehabilitasyon Ekibinde Koruyucu Yaklaşımların Yasal Durumu ve Türkiye’deki Gelişimi Reyhan ÖZGÖBEK Sağlığın Korunması ve Geliştirilmesi Nadire ERCAN TOPTANER, Merve KOLCU Yaşam Kalitesi Nurhayat KORKMAZ Rehabilitasyonda Terapist-Danışan Dengesi: Harmoni, Karşılıklılık, Sürdürebilirlik Yeşim ÜNVEREN Koruyucu Egzersizler Hülya YÜCEL, Ayça ARACI Koruyucu Akuaterapi Hülya YÜCEL Spor Yaralanmalarında Koruyucu Yaklaşımlar Serkan UZLAŞIR Gövde ve Üst Ekstremitede Kullanılan Koruyucu Ortezler ve Destek Ekipmanları Mustafa SARI, Hasan Erkan KILINÇ, Nilgün BEK Ayak ve Alt Ekstremitede Kullanılan Koruyucu Ortezler veDestek Ekipmanları Banu ÜNVER, Mustafa SARI, Nilgün BEK Stres Yönetimi Nurhayat KORKMAZ Yeterli ve Düzenli Uyku Büşra KAPLAN, Hülya YÜCEL Müzik Terapi Hüseyin SÜER Ruh Sağlığını Güçlendirme ve Koruma (Psikolojik Sağlamlık) Selma ERCAN DOĞU Manevi/Spirütüel Korunma Abdulhakim BEKİ, Feyza BEKİ, İsmail BARIŞ Mental/Kognitif Sağlığı Koruma Umut BAHÇACI, Songül ATASAVUN UYSAL Sağlık Hizmetlerinde Mobbing (Yıldırma) ve Korunma Yolları Işık KARAKAYA Madde Bağımlılığından Korunma Yolları Hatice ÖNER, Hülya ARSLANTAŞ Teknoloji Bağımlılığında Koruyucu Yaklaşımlar Büşra CANDİRİ, Burcu TALU Koruyucu Beslenme: Sağlıklı Beslenme Modelleri Günsu SOYKUT, Serpil ÖZSOY Koruyucu ve Bütüncül Yaklaşımda Fitoterapi ve Aromaterapi Özlem SU KÜÇÜK, Nazan TAŞLIDERE Deri Sağlığını Koruma Dilek BIYIK ÖZKAYA, Hülya YÜCEL Kanserde Koruyucu Yaklaşımlar İdil Esin ÜNLÜ, Ece UYSAL ALTUNBİLEK, İlke KESER Bulaşıcı Olmayan (Kronik) Hastalıklardan Korunma Zeynep BAHADIR AĞCE Yaşlılık Döneminde Koruyucu Sağlık Hizmetleri Yasemin KAVLAK, Selda YILDIZ Erkek Sağlığında Koruyucu Yaklaşımlar Ümit ERKUT, İbrahim KÜÇÜKCAN Cinsel Hastalıklardan Korunma Yolları Ayşe Nur AKSOY Diş Çürükleri ve Dişeti Hastalıklarından Korunma Yolları Başak BIYIKOĞLU, Meltem MERT EREN Bruksizm ve Bruksizmden Korunma Yolları Ayşe ATAY, Demet Çağıl AYVALIOĞLU Dil ve Konuşma Bozukluklarında Koruyucu Yaklaşımlar Ahsen ERİM, Şaziye SEÇKİN YILMAZ Sağlıklı ve Güvenli Mekan Tasarımı Uygulamaları Timur KAPROL İç Mekan Tasarımlarında Önleyici Yaklaşımlar Ali MÜLAYİM Erişilebilirlik ve Evrensel Tasarım Buse GÖKMEN KARAGEYİK İş Yerlerinde Ergonomik Yaklaşımlar İlknur YAŞAR, Hülya YÜCEL Farklı İş Kollarında Koruyucu Yaklaşımlar Melike ÖZÇELİK, Hatice ABAOĞLU Laboratuvar Güvenliği ve Deney Hayvanları ile Yapılan Laboratuvar Çalışmalarında Korunma Ülkan KILIÇ Çevresel Atık Farkındalığı ve Su Okuryazarlığı Nazihan URSAVAŞ Doğanın Koruyuculuğu Ömer DİLEK Koruyucu İlk Yardım Demet SEMİZ, Ali ARSLANOĞLU

Objective: To examine the relationship between physical activity level and healthy lifestyle behaviours of white-collar workers. Materials and Methods: The International Physical Activity Questionnaire Short Form (IPAQ-SF) was used to determine the level of physical activity, and the Healthy Lifestyle Behaviours Scale-II (HLBS-II) was used to evaluate the lifestyle behaviours. The researchers recorded anthropometric measurements of the participants via face-to-face method and questionnaires were implemented via online forms. Statistical analysis of the study was performed with the IBM SPSS Statistics version 20.0 program. Descriptive analysis was done, and all the data were presented as means and (±) standard deviation (SD). Statistical significance was set at p<0.05. Results: In total, 229 (mean age: 35.75, 158 female) white-collar workers participated in the study. 90.4% of the participants had either undergraduate or postgraduate degrees. 55.5% of the participants were found to be inactive as stated by the IPAQ-SF scores. According to HLBS-II results, active participants had significantly higher exercise, nutrition, and stress management sub-scale scores (p0.05). Overall, active participants with normal BMI had significantly higher HLBS-II scores than overweight and obese participants (p<0.05). Conclusions: Our study shows a positive relationship between physical activity levels and healthy lifestyle behaviours in office work. In order to increase the quality of life of white-collar workers, changing their behaviours such as physical inactivity and unhealthy diet through education intervention on healthy lifestyle holds a vital place.

The conducted study aimed to determine the nutritional status and activity level of white-collar workers. 211 volunteers' demographic information, anthropometric measurements, and 3-day food recording were determined. The scales “Diet Quality Index-International (DQI-I)”, “Healthy Eating Index-2015 (HEI-2015)”, and “Dietary Approaches to Prevent Hypertension (DASH)” were used to determine the quality of the diets. The International Physical Activity Questionnaire-Short Form (IPAQ) was used to calculate the activity level of the participants. The average age of participants were 35.70 ± 9.36, while 70.6% were women. The average diet quality scores for DQI-I, HEI-2015, and DASH were 47.8 ± 9.79, 56.40±14.04, and, 2.70 ±1.29 respectively. The IPAQ average score was calculated as 851.44±1065.01 MET. The IPAQ results showed that only 13.3% of participants had sufficient activity levels. The physical activity level of the men was found to be significantly higher than that of the women (p<0.05). The activity levels of participants with good healthy eating index scores were also found to be statistically higher (p<0.001). In addition, participants with high DQI-I scores had significantly higher healthy eating habits with HEI-2015(p<0.001). The current study showed that white-collar workers had insufficient physical activity levels, however, sufficient levels of physical activity were correlated with better diet quality.

Colchicum pusillum Sieber is a plant from Colchicaceae family that particularly rich in tropolonic alkaloids, colchicine. The aim of the study is to determine the apoptosis induction effect of Colchicum pusillum extract in Colo-741, metastatic colon cancer cells and if so, to determine by which apoptotic pathway. Colchicum pusillum was collected and extracted with ethanol. Apoptotic activities of Colchicum pusillum were investigated by immunocytochemistry using antibodies directed against to caspase-3, cytochrome-c and FasLigand (FasL) in Colo-741 cells. Immunocytochemical staining results stated that Colchicum pusillum extract at 20 µg/mL dose, increased caspase-3 and FasL activity significantly but was not able to activate cytochrome-c activity. The results suggested that Colchicum pusillum extract induced apoptosis in metastatic colon cancer cells via extrinsic apoptotic pathway.