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Rice bran oil is known as wonder oil and it is the most important vegetable oil in Asia. Rice bran oil is extracted from bran that is the outer hard layer of rice. It is an emerging category in edible oil with a lot of nutritional properties and health benefits. Rice bran oil is heart-friendly, boosts up immunity, and prevents from other diseases occurring commonly in Pakistan. The current study aimed to stabilize rice bran oil through different probiotic isolates and to assess the nutritional content of rice bran oil after stabilization. The study was aimed to inactivate naturally occurring lipases that can hydrolyze oil into glycerol and free fatty acid which is a serious problem that gives it a rancid taste and smell. Antilipase activity was used to inactivate naturally occurring lipases that are a huge threat to the stabilization process. The fermentation process utilizes antilipase activity without affecting the nutritional value of oil. Lactobacillus strains were used for the stabilization of rice bran oil. Rice bran oil was extracted in the Soxhlet apparatus. The probiotic lab isolates Lactobacillus delbrueckii S2, Lactobacillus casei S5 and Lactobacillus plantarum S13 were applied to it to increase its shelf life and prevent oxidative rancidity. The extraction temperature of rice bran oil was maintained above 40 °C to inhibit lipase activity. Rice bran oil samples were stored at refrigeration temperature to arrest lipase activity. Probiotics maintained acidic pH to keep oil stabilization. Qualitative analysis was done to confirm rice bran oil stabilization. Determination of Free Fatty Acid (FFA) and saponification value confirmed that oxidative rancidity of rice bran oil was controlled by probiotics. FFA count was less than 10% and Saponification Value (SV) was 180. GC analysis was performed to analyze the FFA profile. Gas Chromatography results have shown 3 fatty acids. Statistical analysis has shown non-significant effect on different incubation temperatures of Lactobacillus isolates. Among the biological methods of stabilization, the use of probiotics is a novel concept and recommended for commercial application.
Haleema Noureen; Sadia Alam; Samha Al Ayoubi; Abdul Qayyum; Shahida Sadiqi; Samia Atiq; Alia Naz; Yamin Bibi; Waqas Ahmed; Muhammad Mumtaz Khan; Shehla Sammi; Muhammad Liaquat; Shakil Ahmad. Mechanism of Rice Bran Lipase Inhibition through Fermentation Activity of Probiotic Bacteria. Saudi Journal of Biological Sciences 2021, 1 .
AMA StyleHaleema Noureen, Sadia Alam, Samha Al Ayoubi, Abdul Qayyum, Shahida Sadiqi, Samia Atiq, Alia Naz, Yamin Bibi, Waqas Ahmed, Muhammad Mumtaz Khan, Shehla Sammi, Muhammad Liaquat, Shakil Ahmad. Mechanism of Rice Bran Lipase Inhibition through Fermentation Activity of Probiotic Bacteria. Saudi Journal of Biological Sciences. 2021; ():1.
Chicago/Turabian StyleHaleema Noureen; Sadia Alam; Samha Al Ayoubi; Abdul Qayyum; Shahida Sadiqi; Samia Atiq; Alia Naz; Yamin Bibi; Waqas Ahmed; Muhammad Mumtaz Khan; Shehla Sammi; Muhammad Liaquat; Shakil Ahmad. 2021. "Mechanism of Rice Bran Lipase Inhibition through Fermentation Activity of Probiotic Bacteria." Saudi Journal of Biological Sciences , no. : 1.
Continuous application of phosphate (P) mineral to soil renders apatite addition during each crop growing season which is of great concern from a sustainable agriculture viewpoint. Use of efficient phosphate solubilizing microbes (PSB) is one of the most effective ways to solubilize this apatite mineral in the soil. The current study targeted hydroxyapatite mines to explore, isolate and characterize efficient P solubilizers to solubilize apatite in the soil. Efficiency of isolated microbes to solubilize rock phosphate (hydroxyapatite) and tri-calcium phosphate (TCP) as well as indole-3-acetic acid (IAA) and 1-aminocyclopropane-1-carboxylate deaminase (ACC) activity were tested. Identification and phylogenetic analysis of bacterial and fungal isolates were carried out by 16S rRNA and internal transcribed spacer (ITS) rDNA sequence analyses, respectively. The isolated bacterial strains were identified as Staphylococcus sp., Bacillus firmus, Bacillus safensis, and Bacillus licheniformis whereas fungal isolates were identified as Penicillium sp. and Penicillium oxalicum. Results showed that the impact of identified strains in combination with three phosphate fertilizers sources (compost, rock phosphate and diammonium phosphate (DAP)) was conspicuous on maize crop grown in pot. Both bacterial and fungal strains increased the P uptake by plants as well as recorded with higher available P in post-harvested soil. Penicillium sp. in combination with compost resulted in maximum P-uptake by plants and post-harvest soil P contents, compared to other combinations of P sources and bio-inoculants. Screening and application of efficient P solubilizers can be a better option to utilize the indigenous phosphate reserves of soil as well as organic amendments for sustainable agriculture.
Awais Qarni; Motsim Billah; Khadim Hussain; Sabir Shah; Waqas Ahmed; Sadia Alam; Aftab Sheikh; Laila Jafri; Asia Munir; Kouser Malik; Naeem Khan. Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture. Sustainability 2021, 13, 2151 .
AMA StyleAwais Qarni, Motsim Billah, Khadim Hussain, Sabir Shah, Waqas Ahmed, Sadia Alam, Aftab Sheikh, Laila Jafri, Asia Munir, Kouser Malik, Naeem Khan. Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture. Sustainability. 2021; 13 (4):2151.
Chicago/Turabian StyleAwais Qarni; Motsim Billah; Khadim Hussain; Sabir Shah; Waqas Ahmed; Sadia Alam; Aftab Sheikh; Laila Jafri; Asia Munir; Kouser Malik; Naeem Khan. 2021. "Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and their Potential Effect for Sustainable Agriculture." Sustainability 13, no. 4: 2151.
The current study was focused on the usage of bio synthesized zinc oxide nanoparticles to increase the tissue culture efficiency of important forage grass Panicum virgatum. Zinc being a micronutrient enhanced the callogenesis and regeneration efficiency of Panicum virgatum at different concentrations. Here, we synthesized zinc oxide nanoparticles through Cymbopogon citratus leaves extract to evaluate the influence of zinc oxide nanoparticles on the quality of plant regeneration in switchgrass. X-ray diffraction (XRD) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) validate phase purity of green synthesize Zinc oxide nanoparticles whereas, electron microscopy (SEM) has illustrated the average size of particle 50±4 nm with hexagonal rod like shape. Energy dispersive Xray (EDS) spectra depict major peaks of Zn (92.68%) while minor peaks refer to Oxygen (7.32%). ZnO NPs demonstrate the incredibly promising results against callogenesis. Biosynthesized ZnO NPs at optimum concentration showed very promising effect on plant regeneration ability. Both the explants, seeds and nodes used in study showed dose dependent response and upon high doses exceeding 40 mg/L the results were recorded negative, whereas at 30 mg/L both explants demonstrate 70 % and 76 % regeneration frequency. The results conclude that zinc oxide nanoparticles enhance plant growth and development. Being one of the essential plant nutrients, ZnO has greatly tailored the nutritive properties at nano-scale.
Saima Shafique; Nyla Jabeen; Khawaja Shafique Ahmad; Samra Irum; Sadaf Anwaar; Naeem Ahmad; Sadia Alam; Muhammad Ilyas; Talha Farooq Khan; Syed Zaheer Hussain. Green Fabricated Zinc Oxide Nanoformulated Media Enhanced Callus Induction and Regeneration Dynamics of Panicum virgatum L. 2020, 1 .
AMA StyleSaima Shafique, Nyla Jabeen, Khawaja Shafique Ahmad, Samra Irum, Sadaf Anwaar, Naeem Ahmad, Sadia Alam, Muhammad Ilyas, Talha Farooq Khan, Syed Zaheer Hussain. Green Fabricated Zinc Oxide Nanoformulated Media Enhanced Callus Induction and Regeneration Dynamics of Panicum virgatum L. . 2020; ():1.
Chicago/Turabian StyleSaima Shafique; Nyla Jabeen; Khawaja Shafique Ahmad; Samra Irum; Sadaf Anwaar; Naeem Ahmad; Sadia Alam; Muhammad Ilyas; Talha Farooq Khan; Syed Zaheer Hussain. 2020. "Green Fabricated Zinc Oxide Nanoformulated Media Enhanced Callus Induction and Regeneration Dynamics of Panicum virgatum L." , no. : 1.
The impact of heavy metal toxicity on the shoot and root lengths, total protein, fiber characteristics, moisture content and nutrient composition of spinach (Spinacia oleracea) was evaluated. Plants were grown in pots containing soil and treated with different concentrations (mg/kg) of lead (Pb; 300, 400 and 500), cadmium (Cd; 0.5, 1 and 1.5) and zinc (Zn; 250, 500, and 700) as well as mixtures of Cd and Pb (0.5/300, 1/400, 1.5/500), Cd and Zn (0.5/250, 1/500, 1.5/700), and Pb and Zn (300/250, 400/500, 500/700). Soil contaminated by long-term irrigation with wastewater containing heavy metals was simulated. An increase in concentrations of heavy metals both individually and as mixtures significantly (p < 0.05) reduced the growth parameters and nutrient contents of S. oleracea. The uptake patterns of heavy metals in mixtures showed antagonistic impacts on each other. The toxicities of the mixtures Cd and Pb, Cd and Zn as well as Pb and Zn were higher than those observed in separate heavy metal applications but less than their additive sums. The toxicity caused by individual heavy metals was the highest for Cd followed by Pb and Zn. The highest toxicity was observed in plants grown in soil contaminated by Cd and Pb.
Naz Alia; Khan Sardar; Muhammad Said; Khalid Salma; Alam Sadia; Siddique Sadaf; Ahmed Toqeer; Scholz Miklas. Toxicity and Bioaccumulation of Heavy Metals in Spinach (Spinacia oleracea) Grown in a Controlled Environment. International Journal of Environmental Research and Public Health 2015, 12, 7400 -7416.
AMA StyleNaz Alia, Khan Sardar, Muhammad Said, Khalid Salma, Alam Sadia, Siddique Sadaf, Ahmed Toqeer, Scholz Miklas. Toxicity and Bioaccumulation of Heavy Metals in Spinach (Spinacia oleracea) Grown in a Controlled Environment. International Journal of Environmental Research and Public Health. 2015; 12 (7):7400-7416.
Chicago/Turabian StyleNaz Alia; Khan Sardar; Muhammad Said; Khalid Salma; Alam Sadia; Siddique Sadaf; Ahmed Toqeer; Scholz Miklas. 2015. "Toxicity and Bioaccumulation of Heavy Metals in Spinach (Spinacia oleracea) Grown in a Controlled Environment." International Journal of Environmental Research and Public Health 12, no. 7: 7400-7416.