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Dr. Silvia Sabbadini
Department of Agricultural, Food and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy

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Research Keywords & Expertise

0 Improving techniques for creating genetic variability in perennial fruit crops
0 In vitro regeneration approaches (organogenesis, somatic embryogenesis) to be applied for mutagenesis and NBTs applications
0 RNAi strategies (HIGS, SIGS)
0 Cisgenesis/Intragenesis
0 Gene editing.

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Cisgenesis/Intragenesis
Gene editing.

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Regular article
Published: 15 June 2021 in New Phytologist
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Plant architecture is central in determining crop yield. In the short-day species strawberry, a crop vegetatively propagated via daughter-plants produced by stolons, fruit yield is further dependent on the trade-off between sexual reproduction (fruits) and asexual reproduction (daughter-plants). Both are largely dependent on meristem identity, which establishes the development of branches, stolons, and inflorescences. Floral initiation and plant architecture are modulated by the balance between two related proteins, FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1). We explored in woodland strawberry the role of the uncharacterized FveFT2 and FveFT3 genes and of the floral repressor FveTFL1 through gene expression analyses, grafting and genetic transformation (overexpression and gene editing). We demonstrate the unusual properties of these genes. FveFT2 is a non-photoperiodic florigen permitting SD flowering and FveTFL1 is the long-hypothesized long-day systemic antiflorigen that contributes, together with FveFT2, to the photoperiodic regulation of flowering. We additionally show that FveFT3 is not a florigen but promotes plant branching when overexpressed, likely through changing axillary meristem fate, thus resulting in a 3.5 fold increase in fruit yield at the expense of stolons. We show that our findings can be translated into improvement of cultivated strawberry where FveFT2 overexpression significantly accelerates flowering.

ACS Style

Amèlia Gaston; Aline Potier; Marie Alonso; Silvia Sabbadini; Frédéric Delmas; Tracey Tenreira; Noé Cochetel; Marc Labadie; Pierre Prévost; Kevin M. Folta; Bruno Mezzetti; Michel Hernould; Christophe Rothan; Béatrice Denoyes. The FveFT2 florigen/ FveTFL1 antiflorigen balance is critical for the control of seasonal flowering in strawberry while FveFT3 modulates axillary meristem fate and yield. New Phytologist 2021, 1 .

AMA Style

Amèlia Gaston, Aline Potier, Marie Alonso, Silvia Sabbadini, Frédéric Delmas, Tracey Tenreira, Noé Cochetel, Marc Labadie, Pierre Prévost, Kevin M. Folta, Bruno Mezzetti, Michel Hernould, Christophe Rothan, Béatrice Denoyes. The FveFT2 florigen/ FveTFL1 antiflorigen balance is critical for the control of seasonal flowering in strawberry while FveFT3 modulates axillary meristem fate and yield. New Phytologist. 2021; ():1.

Chicago/Turabian Style

Amèlia Gaston; Aline Potier; Marie Alonso; Silvia Sabbadini; Frédéric Delmas; Tracey Tenreira; Noé Cochetel; Marc Labadie; Pierre Prévost; Kevin M. Folta; Bruno Mezzetti; Michel Hernould; Christophe Rothan; Béatrice Denoyes. 2021. "The FveFT2 florigen/ FveTFL1 antiflorigen balance is critical for the control of seasonal flowering in strawberry while FveFT3 modulates axillary meristem fate and yield." New Phytologist , no. : 1.

Correction
Published: 02 June 2021 in Journal of Pest Science
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ACS Style

Bruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. Correction to: RNAi, what is its position in crop protection? Journal of Pest Science 2021, 94, 1555 -1555.

AMA Style

Bruno Mezzetti, Guy Smagghe, Salvatore Arpaia, Olivier Christiaens, Antje Dietz-Pfeilstetter, Huw Jones, Kaloyan Kostov, Silvia Sabbadini, Hilde-Gunn Opsahl-Sorteberg, Vera Ventura, Clauvis Nji Tizi Taning, Jeremy Sweet. Correction to: RNAi, what is its position in crop protection? Journal of Pest Science. 2021; 94 (4):1555-1555.

Chicago/Turabian Style

Bruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. 2021. "Correction to: RNAi, what is its position in crop protection?" Journal of Pest Science 94, no. 4: 1555-1555.

Journal article
Published: 29 March 2021 in Plants
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Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNA interference (RNAi) technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. Recent studies showed that the exogenous application of double-stranded RNA (dsRNA) molecules on plants targeting fungal growth and virulence-related genes provided disease attenuation of pathogens like Botrytis cinerea, Sclerotinia sclerotiorum and Fusarium graminearum in different hosts. Such results highlight that the exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control. Production of dsRNA can be possible by using either in-vitro or in-vivo synthesis. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss dsRNA production, formulation, and RNAi delivery methods. Potential challenges that are faced while developing a RNAi strategy for fungal pathogens, such as off-target and epigenetic effects, with their possible solutions are also discussed.

ACS Style

Daniel Gebremichael; Zeraye Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. Plants 2021, 10, 650 .

AMA Style

Daniel Gebremichael, Zeraye Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. Plants. 2021; 10 (4):650.

Chicago/Turabian Style

Daniel Gebremichael; Zeraye Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. 2021. "RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges." Plants 10, no. 4: 650.

Preprint
Published: 05 March 2021
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Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNAi technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss small RNA production, formulation, and RNAi delivery methods. We explore some challenges with possible solutions. Furthermore, exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control.

ACS Style

Daniel Gebremichael; Zeraye Mehari Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. 2021, 1 .

AMA Style

Daniel Gebremichael, Zeraye Mehari Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi. RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. . 2021; ():1.

Chicago/Turabian Style

Daniel Gebremichael; Zeraye Mehari Haile; Francesca Negrini; Silvia Sabbadini; Luca Capriotti; Bruno Mezzetti; Elena Baraldi. 2021. "RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges." , no. : 1.

Review article
Published: 03 February 2021 in Trends in Food Science & Technology
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The increasing evidences on the relation between diet and human health are driving the consumers toward the choice of foods with high amount of nutrients, considered the molecules responsible for the regulation of several metabolic and biochemical processes, and for the prevention of many chronic diseases. Among food products, fruits represent a natural source of many different nutrients and possess much importance in a balanced diet. The increase of the micro/macronutrient levels and the decrease of antinutrients amount are considered main objectives for the improvement of fruit trees nutritional value. This review summarizes many of the attempts made to increase the nutritional quality of fruit tree species during the last decades through the application of traditional and molecular breeding technologies, and the most recent New breeding techniques (NBTs). New genotypes with improved nutritional quality have been obtained for different fruit tree species, either by increasing the content of well-known beneficial molecules, such as phenolic compounds, vitamins, and carotenoids, or by decreasing the levels of specific antinutrients. The development of molecular techniques and biotechnological tools allowed the identification and validation of candidate genes involved in the regulation of specific classes of nutritional compounds in fruit trees, and useful to obtain nutritionally improved products. NBTs represent alternative tools to classical breeding techniques, to regulate one or more fruit trees key nutrients in a more quick and precise manner. However, genetic engineering approaches still present public and regulatory concerns that strongly limit their exploitation.

ACS Style

Silvia Sabbadini; Franco Capocasa; Maurizio Battino; Luca Mazzoni; Bruno Mezzetti. Improved nutritional quality in fruit tree species through traditional and biotechnological approaches. Trends in Food Science & Technology 2021, 1 .

AMA Style

Silvia Sabbadini, Franco Capocasa, Maurizio Battino, Luca Mazzoni, Bruno Mezzetti. Improved nutritional quality in fruit tree species through traditional and biotechnological approaches. Trends in Food Science & Technology. 2021; ():1.

Chicago/Turabian Style

Silvia Sabbadini; Franco Capocasa; Maurizio Battino; Luca Mazzoni; Bruno Mezzetti. 2021. "Improved nutritional quality in fruit tree species through traditional and biotechnological approaches." Trends in Food Science & Technology , no. : 1.

Journal article
Published: 12 January 2021 in Biomolecules
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Plant-derived exosome-like nanovesicles (EPDENs) have recently been isolated and evaluated as potential bioactive nutraceutical biomolecules. It has been hypothesized that EPDENs may exert their activity on mammalian cells through their specific cargo. In this study, we isolated and purified EPDENs from the strawberry juice of Fragaria x ananassa (cv. Romina), a new cultivar characterized by a high content of anthocyanins, folic acid, flavonols, and vitamin C and an elevated antioxidant capacity. Fragaria-derived EPDENs were purified by a series of centrifugation and filtration steps. EPDENs showed size and morphology similar to mammalian extracellular nanovesicles. The internalization of Fragaria-derived EPDENs by human mesenchymal stromal cells (MSCs) did not negatively affect their viability, and the pretreatment of MSCs with Fragaria-derived EPDENs prevented oxidative stress in a dose-dependent manner. This is possibly due to the presence of vitamin C inside the nanovesicle membrane. The analysis of EPDEN cargo also revealed the presence of small RNAs and miRNAs. These findings suggest that Fragaria-derived EPDENs may be considered nanoshuttles contained in food, with potential health-promoting activity.

ACS Style

Francesca Perut; Laura Roncuzzi; Sofia Avnet; Annamaria Massa; Nicoletta Zini; Silvia Sabbadini; Francesca Giampieri; Bruno Mezzetti; Nicola Baldini. Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells. Biomolecules 2021, 11, 87 .

AMA Style

Francesca Perut, Laura Roncuzzi, Sofia Avnet, Annamaria Massa, Nicoletta Zini, Silvia Sabbadini, Francesca Giampieri, Bruno Mezzetti, Nicola Baldini. Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells. Biomolecules. 2021; 11 (1):87.

Chicago/Turabian Style

Francesca Perut; Laura Roncuzzi; Sofia Avnet; Annamaria Massa; Nicoletta Zini; Silvia Sabbadini; Francesca Giampieri; Bruno Mezzetti; Nicola Baldini. 2021. "Strawberry-Derived Exosome-Like Nanoparticles Prevent Oxidative Stress in Human Mesenchymal Stromal Cells." Biomolecules 11, no. 1: 87.

Review
Published: 09 August 2020 in International Journal of Molecular Sciences
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Downy mildew, powdery mildew, and grey mold are some of the phytopathological diseases causing economic losses in agricultural crops, including grapevine, worldwide. In the current scenario of increasing global warming, in which the massive use of agrochemicals should be limited, the management of fungal disease has become a challenge. The knowledge acquired on candidate resistant (R) genes having an active role in plant defense mechanisms has allowed numerous breeding programs to integrate these traits into selected cultivars, even though with some limits in the conservation of the proper qualitative characteristics of the original clones. Given their gene-specific mode of action, biotechnological techniques come to the aid of breeders, allowing them to generate simple and fast modifications in the host, without introducing other undesired genes. The availability of efficient gene transfer procedures in grapevine genotypes provide valid tools that support the application of new breeding techniques (NBTs). The expertise built up over the years has allowed the optimization of these techniques to overexpress genes that directly or indirectly limit fungal and oomycetes pathogens growth or silence plant susceptibility genes. Furthermore, the downregulation of pathogen genes which act as virulence effectors by exploiting the RNA interference mechanism, represents another biotechnological tool that increases plant defense. In this review, we summarize the most recent biotechnological strategies optimized and applied on Vitis species, aimed at reducing their susceptibility to the most harmful fungal and oomycetes diseases. The best strategy for combating pathogenic organisms is to exploit a holistic approach that fully integrates all these available tools.

ACS Style

Luca Capriotti; Elena Baraldi; Bruno Mezzetti; Cecilia Limera; Silvia Sabbadini. Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine. International Journal of Molecular Sciences 2020, 21, 5701 .

AMA Style

Luca Capriotti, Elena Baraldi, Bruno Mezzetti, Cecilia Limera, Silvia Sabbadini. Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine. International Journal of Molecular Sciences. 2020; 21 (16):5701.

Chicago/Turabian Style

Luca Capriotti; Elena Baraldi; Bruno Mezzetti; Cecilia Limera; Silvia Sabbadini. 2020. "Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine." International Journal of Molecular Sciences 21, no. 16: 5701.

Review
Published: 31 July 2020 in Plants
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Almost 30 years have passed since the first publication reporting regeneration of transformed peach plants. Nevertheless, the general applicability of genetic transformation of this species has not yet been established. Many strategies have been tested in order to obtain an efficient peach transformation system. Despite the amount of time and the efforts invested, the lack of success has significantly limited the utility of peach as a model genetic system for trees, despite its relatively short generation time; small, high-quality genome; and well-studied genetic resources. Additionally, the absence of efficient genetic transformation protocols precludes the application of many biotechnological tools in peach breeding programs. In this review, we provide an overview of research on regeneration and genetic transformation in this species and summarize novel strategies and procedures aimed at producing transgenic peaches. Promising future approaches to develop a robust peach transformation system are discussed, focusing on the main bottlenecks to success including the low efficiency of A. tumefaciens-mediated transformation, the low level of correspondence between cells competent for transformation and those that have regenerative competence, and the high rate of chimerism in the few shoots that are produced following transformation.

ACS Style

Angela Ricci; Silvia Sabbadini; Humberto Prieto; Isabel Mg Padilla; Chris Dardick; Zhijian Li; Ralph Scorza; Cecilia Limera; Bruno Mezzetti; Margarita Perez-Jimenez; Lorenzo Burgos; Cesar Petri. Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants 2020, 9, 971 .

AMA Style

Angela Ricci, Silvia Sabbadini, Humberto Prieto, Isabel Mg Padilla, Chris Dardick, Zhijian Li, Ralph Scorza, Cecilia Limera, Bruno Mezzetti, Margarita Perez-Jimenez, Lorenzo Burgos, Cesar Petri. Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward. Plants. 2020; 9 (8):971.

Chicago/Turabian Style

Angela Ricci; Silvia Sabbadini; Humberto Prieto; Isabel Mg Padilla; Chris Dardick; Zhijian Li; Ralph Scorza; Cecilia Limera; Bruno Mezzetti; Margarita Perez-Jimenez; Lorenzo Burgos; Cesar Petri. 2020. "Genetic Transformation in Peach (Prunus persica L.): Challenges and Ways Forward." Plants 9, no. 8: 971.

Journal article
Published: 16 June 2020 in Plants
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In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP). The influences on leaf regeneration of the ethylene inhibitor silver thiosulphate (STS) and of different combinations of antibiotics added to the optimized regeneration medium were also investigated. The use of 10 μM STS or carbenicillin (238 mM) combined with cefotaxime (210 μM) significantly increased the average number of regenerating shoots per leaf compared to the control. In vitro shoots were finally elongated, rooted and successfully acclimatized in the greenhouse. The results achieved in this study advances the knowledge on factors affecting leaf organogenesis in Prunus spp., and the regeneration protocol described looks promising for the optimization of new genetic transformation procedures in Hansen 536 and other peach rootstocks and cultivars.

ACS Style

Angela Ricci; Luca Capriotti; Bruno Mezzetti; Oriano Navacchi; Silvia Sabbadini. Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536. Plants 2020, 9, 1 .

AMA Style

Angela Ricci, Luca Capriotti, Bruno Mezzetti, Oriano Navacchi, Silvia Sabbadini. Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536. Plants. 2020; 9 (6):1.

Chicago/Turabian Style

Angela Ricci; Luca Capriotti; Bruno Mezzetti; Oriano Navacchi; Silvia Sabbadini. 2020. "Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536." Plants 9, no. 6: 1.

Rapid communication
Published: 26 May 2020 in Journal of Pest Science
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RNA interference (RNAi) is being developed and exploited to improve plants by modifying endogenous gene expression as well as to target pest and pathogen genes both within plants (i.e. host-induced gene silencing) and/or as topical applications (e.g. spray-induced gene silencing). RNAi is a natural mechanism which can be exploited to make a major contribution towards integrated pest management and sustainable agricultural strategies needed worldwide to secure current and future food production. RNAi plants are being assessed and regulated using existing regulatory frameworks for GMO. However, there is an urgent need to develop appropriate science-based risk assessment procedures for topical RNAi applications within existing plant protection products legislation.

ACS Style

Bruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. RNAi: What is its position in agriculture? Journal of Pest Science 2020, 93, 1125 -1130.

AMA Style

Bruno Mezzetti, Guy Smagghe, Salvatore Arpaia, Olivier Christiaens, Antje Dietz-Pfeilstetter, Huw Jones, Kaloyan Kostov, Silvia Sabbadini, Hilde-Gunn Opsahl-Sorteberg, Vera Ventura, Clauvis Nji Tizi Taning, Jeremy Sweet. RNAi: What is its position in agriculture? Journal of Pest Science. 2020; 93 (4):1125-1130.

Chicago/Turabian Style

Bruno Mezzetti; Guy Smagghe; Salvatore Arpaia; Olivier Christiaens; Antje Dietz-Pfeilstetter; Huw Jones; Kaloyan Kostov; Silvia Sabbadini; Hilde-Gunn Opsahl-Sorteberg; Vera Ventura; Clauvis Nji Tizi Taning; Jeremy Sweet. 2020. "RNAi: What is its position in agriculture?" Journal of Pest Science 93, no. 4: 1125-1130.

Chapter
Published: 03 January 2020 in The First Outstanding 50 Years of “Università Politecnica delle Marche”
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The research for sustainable crop production is mandatory to face climate change and reduction of genetic biodiversity and soil fertility in farming systems and to increase food security, food safety and ecosystem services. Evolutionary history of crop species, effects of crop domestication and adaptation to different environments on crop population and identification of genes/genomic regions that control important agronomic and adaptive traits were studied. Yield and quality have been primary aims for our breeders, who also strived to anticipate the future needs of the society, developing resilient varieties to environment, pests and diseases by using traditional and biotechnological tools. Innovation in vineyard and orchard management was introduced by developing new strategies or adapting traditional techniques to the needs of social and climate changes. Cultivated landscape preservation was pursued by improving carbon sequestration, increasing soil fertility and reducing the risks of soil erosion and nitrogen leaching. Studies on social support and consumer appreciation of sustainable farming systems marked the importance of matching food safety and security, conservation of biodiversity and improvement of ecosystem services. These last at farm and landscape scale are the framework approach for describing the benefits of crop production to human well-being.

ACS Style

Davide Neri; Oriana Silvestroni; Nora Baldoni; Matteo Belletti; Elisa Bellucci; Elena Bitocchi; Franco Capocasa; Paride D’Ottavio; Matteo Francioni; Danilo Gambelli; Vania Lanari; Tania Lattanzi; Francesca Massetani; Laura Nanni; Roberto Papa; Serena Polverigiani; Silvia Sabbadini; Marco Toderi; Laura Trozzo. Sustainable Crop Production. The First Outstanding 50 Years of “Università Politecnica delle Marche” 2020, 583 -600.

AMA Style

Davide Neri, Oriana Silvestroni, Nora Baldoni, Matteo Belletti, Elisa Bellucci, Elena Bitocchi, Franco Capocasa, Paride D’Ottavio, Matteo Francioni, Danilo Gambelli, Vania Lanari, Tania Lattanzi, Francesca Massetani, Laura Nanni, Roberto Papa, Serena Polverigiani, Silvia Sabbadini, Marco Toderi, Laura Trozzo. Sustainable Crop Production. The First Outstanding 50 Years of “Università Politecnica delle Marche”. 2020; ():583-600.

Chicago/Turabian Style

Davide Neri; Oriana Silvestroni; Nora Baldoni; Matteo Belletti; Elisa Bellucci; Elena Bitocchi; Franco Capocasa; Paride D’Ottavio; Matteo Francioni; Danilo Gambelli; Vania Lanari; Tania Lattanzi; Francesca Massetani; Laura Nanni; Roberto Papa; Serena Polverigiani; Silvia Sabbadini; Marco Toderi; Laura Trozzo. 2020. "Sustainable Crop Production." The First Outstanding 50 Years of “Università Politecnica delle Marche” , no. : 583-600.

Perspective
Published: 14 October 2019 in Pest Management Science
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Facing current climate challenges and drastically reduced chemical options for plant protection, the exploitation of RNA interference (RNAi) as an agricultural biotechnology tool has unveiled possible new solutions to the global problems of agricultural losses caused by pests and other biotic and abiotic stresses. While the use of RNAi as a tool in agriculture is still limited to a few transgenic crops, and only adopted in restricted parts of the world, scientists and industry are already seeking innovations in leveraging and exploiting the potential of RNAi in the form of RNA‐based biocontrol compounds for external applications. Here, we highlight the expanding research and development pipeline, commercial landscape and regulatory environment surrounding the pursuit of RNA‐based biocontrol compounds with improved environmental profiles. The commitments of well‐established agrochemical companies to invest in research endeavours and the role of start‐up companies are crucial for the successful development of practical applications for these compounds. Additionally, the availability of standardized guidelines to tackle regulatory ambiguities surrounding RNA‐based biocontrol compounds will help to facilitate the entire commercialization process. Finally, communication to create awareness and public acceptance will be key to the deployment of these compounds. This article is protected by copyright. All rights reserved.

ACS Style

Clauvis Nji Tizi Taning; Salvatore Arpaia; Olivier Christiaens; Antje Dietz‐Pfeilstetter; Huw Jones; Bruno Mezzetti; Silvia Sabbadini; Hilde‐Gunn Sorteberg; Jeremy Sweet; Vera Ventura; Guy Smagghe. RNA‐based biocontrol compounds: current status and perspectives to reach the market. Pest Management Science 2019, 76, 841 -845.

AMA Style

Clauvis Nji Tizi Taning, Salvatore Arpaia, Olivier Christiaens, Antje Dietz‐Pfeilstetter, Huw Jones, Bruno Mezzetti, Silvia Sabbadini, Hilde‐Gunn Sorteberg, Jeremy Sweet, Vera Ventura, Guy Smagghe. RNA‐based biocontrol compounds: current status and perspectives to reach the market. Pest Management Science. 2019; 76 (3):841-845.

Chicago/Turabian Style

Clauvis Nji Tizi Taning; Salvatore Arpaia; Olivier Christiaens; Antje Dietz‐Pfeilstetter; Huw Jones; Bruno Mezzetti; Silvia Sabbadini; Hilde‐Gunn Sorteberg; Jeremy Sweet; Vera Ventura; Guy Smagghe. 2019. "RNA‐based biocontrol compounds: current status and perspectives to reach the market." Pest Management Science 76, no. 3: 841-845.

Journal article
Published: 17 June 2019 in Plants
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Prunus spp. is one of the most recalcitrant fruit tree species in terms of in vitro regeneration and transformation, mostly when mature tissues are used as explants. The present study describes the in vitro regeneration via indirect organogenesis, and Agrobacterium tumefaciens-mediated transformation of the peach rootstock Hansen 536 (Prunus persica × Prunus amygdalus) through the use of meristematic bulks (MBs) as starting explants. Efficient adventitious shoot regeneration was obtained when Hansen 536 MBs were cultured on an optimized medium consisting of modified McCown Woody Plant medium (WPM) enriched with 4.4 M 6-Benzyladenine (BA), 0.1 M 1-Naphthaleneacetic acid (NAA) and 6.0 g L−1 plant agar S1000 (B&V). MB slices were used later as starting explants for Agrobacterium-mediated transformation to introduce an RNAi construct “ihp35S-PPV194” against PPV virus. Transgenic events were identified by both green fluorescent protein (GFP) screening and kanamycin selection at different concentrations (0, 17 or 42 M). GFP-fluorescent proliferating callus lines were selected and confirmed to stably express the ihp35S-PPV194::eGFP gene construct by molecular analysis. Although shoot regeneration from these transgenic calli has not been obtained yet, this represents one of the few examples of successful attempts in peach genetic transformation from somatic tissues, and also serves as a useful in vitro system for future gene functional analysis in peach.

ACS Style

Silvia Sabbadini; Angela Ricci; Cecilia Limera; Dania Baldoni; Luca Capriotti; Bruno Mezzetti. Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus. Plants 2019, 8, 178 .

AMA Style

Silvia Sabbadini, Angela Ricci, Cecilia Limera, Dania Baldoni, Luca Capriotti, Bruno Mezzetti. Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus. Plants. 2019; 8 (6):178.

Chicago/Turabian Style

Silvia Sabbadini; Angela Ricci; Cecilia Limera; Dania Baldoni; Luca Capriotti; Bruno Mezzetti. 2019. "Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus." Plants 8, no. 6: 178.

Journal article
Published: 24 January 2019 in Scientific Reports
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The success of in vitro plant regeneration and the competence of genetic transformation greatly depends on the genotype of the species of interest. In previous work, we developed a method for the efficient Agrobacterium-mediated genetic transformation via organogenesis of V. vinifera cultivar Thompson Seedless, by using meristematic bulk (MB) as starting tissue. In this study, we applied this method for the regeneration and transformation of MBs obtained from the Italian cultivar Ciliegiolo and two of the commonly used Vitis rootstocks, 110 Richter and Kober 5BB, in comparison with Thompson Seedless. The A. tumefaciens strain EHA105, harbouring pK7WG2 binary vector, was used for the transformation trials, which allowed selection through the enhanced-green fluorescent protein (eGFP) and the neomycin phosphotransferase (nptII) gene. Putative transformed tissues and/or shoots were identified by either a screening based on the eGFP expression alone or its use in combination with kanamycin in the medium. MBs obtained from Thompson Seedless showed the highest regeneration and transformation cell competence, which subsequently allowed the recovery of stably transformed plants. Ciliegiolo, 110 Richter, and Kober 5BB, produced actively growing transgenic calli showing eGFP fluorescence, more consistently on selective media, but had no regenerative competence.

ACS Style

S. Sabbadini; L. Capriotti; B. Molesini; T. Pandolfini; O. Navacchi; C. Limera; A. Ricci; B. Mezzetti. Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis. Scientific Reports 2019, 9, 582 .

AMA Style

S. Sabbadini, L. Capriotti, B. Molesini, T. Pandolfini, O. Navacchi, C. Limera, A. Ricci, B. Mezzetti. Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis. Scientific Reports. 2019; 9 (1):582.

Chicago/Turabian Style

S. Sabbadini; L. Capriotti; B. Molesini; T. Pandolfini; O. Navacchi; C. Limera; A. Ricci; B. Mezzetti. 2019. "Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis." Scientific Reports 9, no. 1: 582.

Research article
Published: 08 January 2018 in Journal of Agricultural and Food Chemistry
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Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.

ACS Style

Francesca Giampieri; Massimiliano Gasparrini; Tamara Y. Forbes-Hernandez; Luca Mazzoni; Franco Capocasa; Silvia Sabbadini; Josè M. Alvarez-Suarez; Sadia Afrin; Carlo Rosati; Tiziana Pandolfini; Barbara Molesini; José Federico Sánchez Sevilla; Iraida Amaya; Bruno Mezzetti; Maurizio Battino. Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. Journal of Agricultural and Food Chemistry 2018, 66, 581 -592.

AMA Style

Francesca Giampieri, Massimiliano Gasparrini, Tamara Y. Forbes-Hernandez, Luca Mazzoni, Franco Capocasa, Silvia Sabbadini, Josè M. Alvarez-Suarez, Sadia Afrin, Carlo Rosati, Tiziana Pandolfini, Barbara Molesini, José Federico Sánchez Sevilla, Iraida Amaya, Bruno Mezzetti, Maurizio Battino. Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. Journal of Agricultural and Food Chemistry. 2018; 66 (3):581-592.

Chicago/Turabian Style

Francesca Giampieri; Massimiliano Gasparrini; Tamara Y. Forbes-Hernandez; Luca Mazzoni; Franco Capocasa; Silvia Sabbadini; Josè M. Alvarez-Suarez; Sadia Afrin; Carlo Rosati; Tiziana Pandolfini; Barbara Molesini; José Federico Sánchez Sevilla; Iraida Amaya; Bruno Mezzetti; Maurizio Battino. 2018. "Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells." Journal of Agricultural and Food Chemistry 66, no. 3: 581-592.

Review article
Published: 15 August 2017 in Frontiers in Plant Science
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The improvement of woody fruit species by traditional plant breeding techniques has several limitations mainly caused by their high degree of heterozygosity, the length of their juvenile phase and auto-incompatibility. The development of new biotechnological tools (NBTs), such as RNA interference (RNAi), trans-grafting, cisgenesis/intragenesis and genome editing tools, like zinc-finger and CRISPR/Cas9, has introduced the possibility of more precise and faster genetic modifications of plants. This aspect is of particular importance for the introduction or modification of specific traits in woody fruit species while maintaining unchanged the general characteristics of a selected cultivar. Moreover, some of these new tools give the possibility to obtain transgene-free modified fruit tree genomes, which should increase consumer’s acceptance. Over the decades biotechnological tools have undergone rapid development and there is a continuous addition of new and valuable techniques for plant breeders. This makes it possible to create desirable woody fruit varieties in a fast and more efficient way to meet the demand for sustainable agricultural productivity. Although NBTs have a common goal i.e. precise, fast and efficient crop improvement, individually they are markedly different in approach and characteristics from each other. In this review we describe in detail their mechanisms and applications for the improvement of fruit trees and consider the relationship between these biotechnological tools and the EU biosafety regulations applied to the plants and products obtained through these techniques.

ACS Style

Cecilia Limera; Silvia Sabbadini; Jeremy B. Sweet; Bruno Mezzetti. New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species. Frontiers in Plant Science 2017, 8, 1 .

AMA Style

Cecilia Limera, Silvia Sabbadini, Jeremy B. Sweet, Bruno Mezzetti. New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species. Frontiers in Plant Science. 2017; 8 ():1.

Chicago/Turabian Style

Cecilia Limera; Silvia Sabbadini; Jeremy B. Sweet; Bruno Mezzetti. 2017. "New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species." Frontiers in Plant Science 8, no. : 1.

Journal article
Published: 01 August 2016 in Scientia Horticulturae
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Application of in vitro plant biotechnology in berry crops depends on the availability of efficient regeneration protocols that are specific to the genotype background and to the correct combination of exogenous hormones (auxin and cytokinin) added to the medium. Strawberry regeneration protocols are available for different cultivars, while more limited information are available for blueberry cultivars. In this study, we show that the best regeneration efficiency for the leaves of strawberry cultivar Calypso were obtained culturing in a medium supplemented with thidiazuron (TDZ) 0.5 mg L−1 and 2,4-dichlorophenoxyacetic acid (2,4-D) 0.02 mg L−1. The best regeneration efficiency for cultivar Sveva leaves was obtained culturing in a medium supplemented with N6-benzyladenine (BA) 3 mg L−1 and indole-3-butyric acid (IBA) 0.2 mg L−1. In blueberry cultivar Duke, shoot proliferation trials were carried out comparing the effects of 2-isopentenyladenine (2iP) and TDZ; furthermore, experiments of blueberry direct and indirect organogenesis were made, using TDZ as alternatives to zeatin, the most common and expensive hormone used for blueberry in vitro regeneration. Different regeneration responses were observed by using TDZ alone or combined with 2iP. The addition of both 0.2 mg L−1 or 0.5 mg L−1 of TDZ in the medium led to improved callus formation. The addition of 15 mg L−1 of 2iP in the same medium promoted blueberry stem elongation, while inhibiting callus growth. Zeatin was most efficient in direct regeneration of shoots while 0.5 mg L−1 TDZ induced a highest number of shoots by indirect organogenesis.

ACS Style

Roberto Cappelletti; Silvia Sabbadini; Bruno Mezzetti. The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Scientia Horticulturae 2016, 207, 117 -124.

AMA Style

Roberto Cappelletti, Silvia Sabbadini, Bruno Mezzetti. The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars. Scientia Horticulturae. 2016; 207 ():117-124.

Chicago/Turabian Style

Roberto Cappelletti; Silvia Sabbadini; Bruno Mezzetti. 2016. "The use of TDZ for the efficient in vitro regeneration and organogenesis of strawberry and blueberry cultivars." Scientia Horticulturae 207, no. : 117-124.

Journal article
Published: 01 February 2016 in Acta Horticulturae
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ACS Style

Bruno Mezzetti; S. Sabbadini; I. Pertry; S. Burssens; Y. Lakko. Biosafety capacity building: experiences and challenges of a distance learning approach. Acta Horticulturae 2016, 211 -214.

AMA Style

Bruno Mezzetti, S. Sabbadini, I. Pertry, S. Burssens, Y. Lakko. Biosafety capacity building: experiences and challenges of a distance learning approach. Acta Horticulturae. 2016; (1110):211-214.

Chicago/Turabian Style

Bruno Mezzetti; S. Sabbadini; I. Pertry; S. Burssens; Y. Lakko. 2016. "Biosafety capacity building: experiences and challenges of a distance learning approach." Acta Horticulturae , no. 1110: 211-214.

Journal article
Published: 01 February 2016 in Acta Horticulturae
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ISHS XXIX International Horticultural Congress on Horticulture: Sustaining Lives, Livelihoods and Landscapes (IHC2014): International Symposium on Molecular Biology in Horticulture Genetic transformation of peach rootstock and cultivar to induce resistance against PPV virus through post-transcriptional gene silencing

ACS Style

S. Sabbadini; R. Cappelletti; Bruno Mezzetti; T. Pandolfini; B. Molisani; O. Navacchi; C. Crosatti; G. Tacconi. Genetic transformation of peach rootstock and cultivar to induce resistance against PPV virus through post-transcriptional gene silencing. Acta Horticulturae 2016, 223 -228.

AMA Style

S. Sabbadini, R. Cappelletti, Bruno Mezzetti, T. Pandolfini, B. Molisani, O. Navacchi, C. Crosatti, G. Tacconi. Genetic transformation of peach rootstock and cultivar to induce resistance against PPV virus through post-transcriptional gene silencing. Acta Horticulturae. 2016; (1110):223-228.

Chicago/Turabian Style

S. Sabbadini; R. Cappelletti; Bruno Mezzetti; T. Pandolfini; B. Molisani; O. Navacchi; C. Crosatti; G. Tacconi. 2016. "Genetic transformation of peach rootstock and cultivar to induce resistance against PPV virus through post-transcriptional gene silencing." Acta Horticulturae , no. 1110: 223-228.

Book chapter
Published: 20 September 2014 in Advanced Structural Safety Studies
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Until now, the application of genetic transformation techniques in peach has been limited by the difficulties in developing efficient regeneration and transformation protocols. Here we describe an efficient regeneration protocol for the commercial micropropagation of GF677 rootstock (Prunus persica × Prunus amygdalus). The method is based on the production, via organogenesis, of meristematic bulk tissues characterized by a high competence for shoot regeneration.

ACS Style

Silvia Sabbadini; Tiziana Pandolfini; Luca Girolomini; Barbara Molesini; Oriano Navacchi. Peach (Prunus persica L.). Advanced Structural Safety Studies 2014, 1224, 205 -215.

AMA Style

Silvia Sabbadini, Tiziana Pandolfini, Luca Girolomini, Barbara Molesini, Oriano Navacchi. Peach (Prunus persica L.). Advanced Structural Safety Studies. 2014; 1224 ():205-215.

Chicago/Turabian Style

Silvia Sabbadini; Tiziana Pandolfini; Luca Girolomini; Barbara Molesini; Oriano Navacchi. 2014. "Peach (Prunus persica L.)." Advanced Structural Safety Studies 1224, no. : 205-215.