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Vaclav Stejskal's main area of research is pest control (chemical, no-chemical, fumigation, modified- atmospheres, rodenticides), agricultural ecology, arthropods as food contaminants and allergens (food safety). He serves as a head of the Team of pest control and food safety at Crop Res. Inst. Visiting lecturer and scientist among others, in Namibian University, Faculty of Agriculture, Czech Agricultural Univ., Charles University Prague. Vaclav Stejskal is the Czech governmental representative for Plant Health Network of EFSA. He is a founding member and a long serving president (2003-2018) of Scientific Phytosanitary Committee of the CZ (www. phytosanitary. org.). He is a lecturer and examiner for the state certified fumigation national courses in the Czech Republic.
The selective application of insecticides is one of the cornerstones of integrated pest management (IPM) and management strategies for pest resistance to insecticides. The present work provides a comprehensive overview of the traditional and new methods for the application of gas, liquid, gel, and solid physical insecticide formulations to control stored-product and food industry urban pests from the taxa Acarina, Blattodea, Coleoptera, Diptera, Hymenoptera, Lepidoptera, Psocoptera, and Zygentoma. Various definitions and concepts historically and currently used for various pesticide application formulations and methods are also described. This review demonstrates that new technological advances have sparked renewed research interest in the optimization of conventional methods such as insecticide aerosols, sprays, fumigants, and inert gases. Insect growth regulators/disruptors (IGRs/IGDs) are increasingly employed in baits, aerosols, residual treatments, and as spray-residual protectants for long-term stored-grain protection. Insecticide-impregnated hypoxic multilayer bags have been proven to be one of the most promising low-cost and safe methods for hermetic grain storage in developing countries. Insecticide-impregnated netting and food baits were originally developed for the control of urban/medical pests and have been recognized as an innovative technology for the protection of stored commodities. New biodegradable acaricide gel coatings and nets have been suggested for the protection of ham meat. Tablets and satchels represent a new approach for the application of botanicals. Many emerging technologies can be found in the form of impregnated protective packaging (insect growth regulators/disruptors (IGRs/IGDs), natural repellents), pheromone-based attracticides, electrostatic dust or sprays, nanoparticles, edible artificial sweeteners, hydrogels, inert baits with synthetic attractants, biodegradable encapsulations of active ingredients, and cyanogenic protective grain coatings. Smart pest control technologies based on RNA-based gene silencing compounds incorporated into food baits stand at the forefront of current strategic research. Inert gases and dust (diatomaceous earth) are positive examples of alternatives to synthetic pesticide products, for which methods of application and their integration with other methods have been proposed and implemented in practice. Although many promising laboratory studies have been conducted on the biological activity of natural botanical insecticides, published studies demonstrating their effective industrial field usage in grain stores and food production facilities are scarce. This review shows that the current problems associated with the application of some natural botanical insecticides (e.g., sorption, stability, field efficacy, and smell) to some extent echo problems that were frequently encountered and addressed almost 100 years ago during the transition from ancient to modern classical chemical pest control methods.
Vaclav Stejskal; Tomas Vendl; Radek Aulicky; Christos Athanassiou. Synthetic and Natural Insecticides: Gas, Liquid, Gel and Solid Formulations for Stored-Product and Food-Industry Pest Control. Insects 2021, 12, 590 .
AMA StyleVaclav Stejskal, Tomas Vendl, Radek Aulicky, Christos Athanassiou. Synthetic and Natural Insecticides: Gas, Liquid, Gel and Solid Formulations for Stored-Product and Food-Industry Pest Control. Insects. 2021; 12 (7):590.
Chicago/Turabian StyleVaclav Stejskal; Tomas Vendl; Radek Aulicky; Christos Athanassiou. 2021. "Synthetic and Natural Insecticides: Gas, Liquid, Gel and Solid Formulations for Stored-Product and Food-Industry Pest Control." Insects 12, no. 7: 590.
With the increasing importance of soilborne plant pest nematodes and the relatively recent phase-out of methyl bromide as a key soil fumigant, there is an urgent need for new fumigants with good nematicidal properties. Ethanedinitrile (EDN) is a promising fumigant and preparation because of its physical, agrochemical, and nematicidal properties. However, its efficacy against nematode pests of different crops under field conditions has not been fully validated and understood. Therefore, the aim of our study was to evaluate the effect of two concentrations of EDN on the survival of northern root-knot nematodes (Meloidogynehapla) and on carrot yield and quality under field conditions. The evaluation was performed using naturally infested carrot plots, and EDN application to the soil was followed by covering the treated area with film. A high biological effect was observed for both EDN concentrations tested, with even the lower dose (30 g·m−2) being sufficient for M. hapla suppression. Positive effects of EDN application—likely due to EDN partial conversion into biologically available nitrogen in soil—on carrot root weight, in comparison with the untreated control, were also observed.
Ondřej Douda; Marie Manasova; Miloslav Zouhar; Jonas Hnatek; Vaclav Stejskal. Field Validation of the Effect of Soil Fumigation of Ethanedinitrile (EDN) on the Mortality of Meloidogynehapla and Carrot Yield Parameters. Agronomy 2021, 11, 208 .
AMA StyleOndřej Douda, Marie Manasova, Miloslav Zouhar, Jonas Hnatek, Vaclav Stejskal. Field Validation of the Effect of Soil Fumigation of Ethanedinitrile (EDN) on the Mortality of Meloidogynehapla and Carrot Yield Parameters. Agronomy. 2021; 11 (2):208.
Chicago/Turabian StyleOndřej Douda; Marie Manasova; Miloslav Zouhar; Jonas Hnatek; Vaclav Stejskal. 2021. "Field Validation of the Effect of Soil Fumigation of Ethanedinitrile (EDN) on the Mortality of Meloidogynehapla and Carrot Yield Parameters." Agronomy 11, no. 2: 208.
Pyralid moths, Ephestia kuehniella and Plodia interpunctella, are prevalent stored product pests. The insecticides are the main tool to control these months in the stores. The data describing the response of these moths to insecticides are scare. The lethal effect of the organophosphate, pyrethroid, and halogenated‐pyrrole on moths larvae were compared in laboratory test. The hypothesis was that the very polyphagous P. interpunctella would have generally higher insecticide tolerance than that of the stenophagous E. kuehniella. Different insecticide concentrations were applied onto the inner surface of glass tube vials. Ten larvae of 14 or 21 days old of E. kuehniella and 7 or 14 days old of P. interpunctella were used by treatment. The larval mortality was checked after 24 hours of exposure. The mortality was significantly influenced by age of larvae and the groups of chemicals. No differences among the efficacies of the tested formulations with identical active compounds were found, except significant different mortality of E. kuehniella on deltamethrin formulations. A comparison of analytical standards showed that P. interpunctella was less susceptible to the active ingredient pirimiphos‐methyl than E. kuehniella, while E. kuehniella was less susceptible to deltamethrin than P. interpunctella. No differences between the two species were observed for chlorfenapyr. We therefore rejected the hypothesis that polyphagy/stehophagy can be a general predictor of insecticide tolerance in the two tested storage moths. The most important finding for effective use was that the young larvae of both species were more susceptible to tested insecticides than older larvae. This article is protected by copyright. All rights reserved
Vaclav Stejskal; Jitka Stara; Stano Pekar; Marta Nesvorna; Jan Hubert. Sensitivity of polyphagous ( Plodia interpunctella ) and stenophagous ( Ephestia kuehniella ) storage moths to residual insecticides: effect of formulation and larval age. Insect Science 2020, 1 .
AMA StyleVaclav Stejskal, Jitka Stara, Stano Pekar, Marta Nesvorna, Jan Hubert. Sensitivity of polyphagous ( Plodia interpunctella ) and stenophagous ( Ephestia kuehniella ) storage moths to residual insecticides: effect of formulation and larval age. Insect Science. 2020; ():1.
Chicago/Turabian StyleVaclav Stejskal; Jitka Stara; Stano Pekar; Marta Nesvorna; Jan Hubert. 2020. "Sensitivity of polyphagous ( Plodia interpunctella ) and stenophagous ( Ephestia kuehniella ) storage moths to residual insecticides: effect of formulation and larval age." Insect Science , no. : 1.
Although there is growing evidence that arthropod detection methods are not sensitive enough to meet current quality demands, few adequate validations are available for the detection of insect-damaged kernels (IDK- quality parameter). Therefore the aims of this work were to (i) describe categories of exit holes of Sitophilus granarius (L.) in terms of their location on the grain surface; (ii) analyse spatial distribution of exit holes on kernels of three varieties of malting barley; (iii) analyse the sensitivity of visual laboratory methods for IDK detection, and (iv) test if the turning effect of automatic shaking can increase damage visibility from the upper-view perspective of an inspector. Six topological categorises of exit holes were described and photo-documented. It was found that the relative distribution of exit holes on the kernel surface was in decreasing order from lateral, brush, ventral or dorsal (depending on variety), subapical, to germ area. The ventral and dorsal exit holes were significantly bigger than the apical exit holes. It was found that distribution patterns of exit holes on kernels affected their proportional visibility from various observation perspectives. Only 50% of lateral or dorsal exit holes was visible from top or crease views, respectively. The most effective was the side (brush end) view perspective, with visibility of up to 90% of exit holes. Although the shaking effect was statistically significant, it only increased the efficacy of upper view detection perspective from 54% to 58%. This is the first study showing that the inspector upper view of inspected grain samples (without grain rotation) poses a risk of underestimating actual insect-damaged kernels by 50%. Thus, advanced methods that are independent of observation perspectives (X-ray, NIRS, etc.) should be promoted for routine use in commercial laboratories, despite higher cost.
Vaclav Stejskal; Tomas Vendl; Zhihong Li; Radek Aulicky. Efficacy of visual evaluation of insect-damaged kernels of malting barley by Sitophilus granarius from various observation perspectives. Journal of Stored Products Research 2020, 89, 101711 .
AMA StyleVaclav Stejskal, Tomas Vendl, Zhihong Li, Radek Aulicky. Efficacy of visual evaluation of insect-damaged kernels of malting barley by Sitophilus granarius from various observation perspectives. Journal of Stored Products Research. 2020; 89 ():101711.
Chicago/Turabian StyleVaclav Stejskal; Tomas Vendl; Zhihong Li; Radek Aulicky. 2020. "Efficacy of visual evaluation of insect-damaged kernels of malting barley by Sitophilus granarius from various observation perspectives." Journal of Stored Products Research 89, no. : 101711.
Primary pests such as Rhyzoperta dominica may increase the contents of dockage, dust, and frass in grain mass. Although it has been suggested that frass can affect the population growth of stored product pests and ecological interactions among primary and secondary pests in stored grain, this has not been validated experimentally. Therefore, this work experimentally tested the hypothesis that R. dominica wheat frass may support population increases in secondary pests such as Tribolium confusum, T. castaneum, and Oryzaephilus surinamensis for the first time. The effect of frass on secondary pest performance was compared with the effects of various physical qualities of wheat grain (i.e., intact grain kernels, grain fragments, flour, grain + frass) and an artificially enriched control diet (milled wheat kernels, oat flakes, and yeast). The results showed that the clean intact grain kernels did not support the population growth of any tested species, and the nutrient-rich control diet provided the best support. Frass was a significantly better food medium for O. surinamensis and T. castaneum than flour or cracked grain, while T. confusum performed equally well on flour and frass. Our results showed that in terms of food quality and suitability for the tested species, frass occupied an intermediate position between the optimized breeding diet and simple uniform cereal diets such as cracked grain or flour. The results suggest that (i) the wheat frass of primary pest R. dominica is a riskier food source for the development of the tested secondary pests than intact or cracked wheat grain or flour; (ii) frass has the potential to positively influence interspecific interactions between R. dominica and the tested secondary pests; and (iii) wheat grain should be cleaned if increases in R. dominica populations and/or accumulated frass are detected.
J.A. Shah; T. Vendl; R. Aulicky; V. Stejskal. Frass produced by the primary pest Rhyzopertha dominica supports the population growth of the secondary stored product pests Oryzaephilus surinamensis, Tribolium castaneum, and T. confusum. Bulletin of Entomological Research 2020, 111, 153 -159.
AMA StyleJ.A. Shah, T. Vendl, R. Aulicky, V. Stejskal. Frass produced by the primary pest Rhyzopertha dominica supports the population growth of the secondary stored product pests Oryzaephilus surinamensis, Tribolium castaneum, and T. confusum. Bulletin of Entomological Research. 2020; 111 (2):153-159.
Chicago/Turabian StyleJ.A. Shah; T. Vendl; R. Aulicky; V. Stejskal. 2020. "Frass produced by the primary pest Rhyzopertha dominica supports the population growth of the secondary stored product pests Oryzaephilus surinamensis, Tribolium castaneum, and T. confusum." Bulletin of Entomological Research 111, no. 2: 153-159.
At a global scale, the sustainability of forests is endangered by multiple invasive species, including the pine wood nematode (Bursaphelenchus xylophilus), a quarantine pest. International laws and standards require that all exported wood coming from countries in which B. xylophilus is present be chemically or physically treated. Since a major fumigant, methyl bromide, was banned, there has been a need to generate data for alternative fumigants, such as ethanedinitrile (EDN), for this purpose. Since the field screening of fumigants (i.e., the application of various doses to and exposure times of naturally infested wood logs) is prohibitively expensive, the aim of this study was to develop a quick and inexpensive laboratory method. Here, we suggest and describe an innovative method based on sawdust cultures for EDN efficacy screening. In the validation part of this study, we demonstrated (i) the high survival of the nematodes in the sawdust and (ii) the high efficacy of EDN against this pest under in vitro conditions; 100% mortality was observed after 6 h of EDN exposure to a dose of 25 g/m3. In particular, our newly developed model system could be used for the initial screening of various doses of and exposure protocols for EDN and similar types of fumigants developed with the intention of regulating B. xylophilus occurrence in exported wood. It is believed that the validated method may help to develop new and effective EDN fumigation procedures and thereby contribute to the long-term protection of forests worldwide.
Ondřej Douda; Václav Stejskal; Marie Manasova; Miloslav Zouhar; Jonáš Hnatek. Inexpensive Screening Method to Validate the Efficacy of Ethanedinitrile Fumigant on the Forest Invasive Nematode Pest Bursaphelenchus xylophilus. Sustainability 2020, 12, 4765 .
AMA StyleOndřej Douda, Václav Stejskal, Marie Manasova, Miloslav Zouhar, Jonáš Hnatek. Inexpensive Screening Method to Validate the Efficacy of Ethanedinitrile Fumigant on the Forest Invasive Nematode Pest Bursaphelenchus xylophilus. Sustainability. 2020; 12 (11):4765.
Chicago/Turabian StyleOndřej Douda; Václav Stejskal; Marie Manasova; Miloslav Zouhar; Jonáš Hnatek. 2020. "Inexpensive Screening Method to Validate the Efficacy of Ethanedinitrile Fumigant on the Forest Invasive Nematode Pest Bursaphelenchus xylophilus." Sustainability 12, no. 11: 4765.
The quality and type of food packaging affect the level of food protection against pests. This work first evaluated the effect of package perforations on the infestation of cereal-fruit bars by the eggs of the Indian meal moth (Plodia interpunctella). We measured the differential oviposition of moths on the unpackaged bars, empty packages, bars in packages and bars in perforated packages in choice and no-choice experiments. Almost 100% of the laid eggs were laid directly on the bars when they were placed in the enclosure without packaging. A low proportion of the eggs (0.4–3%) were laid on either the empty or non-perforated polypropylene foil packages. Plodia interpunctella efficiently located and infested the bars with eggs when 5 mm package perforations were present in no-choice test. In choice test P. interpunctella preferred to oviposit on open bars than on the packaged or perforated bars. After deposition on the bars the egg hatchability ranged between 40 and 74%. For the food industry, the most significant practical conclusions of this study are that non-perforated packages provide protection against oviposition of P. interpunctella, but small perforations enable the moth to infest the resource with eggs.
R. Aulicky; T. Vendl; Vaclav Stejskal. Evaluation of contamination of packages containing cereal-fruit bars by eggs of the pest Indian meal moth (Plodia interpunctella, Lepidoptera) due to perforations in their polypropylene foil packaging. Journal of Food Science and Technology 2019, 56, 3293 -3299.
AMA StyleR. Aulicky, T. Vendl, Vaclav Stejskal. Evaluation of contamination of packages containing cereal-fruit bars by eggs of the pest Indian meal moth (Plodia interpunctella, Lepidoptera) due to perforations in their polypropylene foil packaging. Journal of Food Science and Technology. 2019; 56 (7):3293-3299.
Chicago/Turabian StyleR. Aulicky; T. Vendl; Vaclav Stejskal. 2019. "Evaluation of contamination of packages containing cereal-fruit bars by eggs of the pest Indian meal moth (Plodia interpunctella, Lepidoptera) due to perforations in their polypropylene foil packaging." Journal of Food Science and Technology 56, no. 7: 3293-3299.
Low temperatures play an important role in arthropods because they affect both the individual and population development of all physiological and behavioural activities. Manipulation with low temperatures is a primary nonchemical pest control method. For stored product and food industry practitioners, a knowledge of pest thermal requirements, in particular threshold temperatures at which development and other activities of a particular pest species cease, is of crucial importance. This review presents summary data regarding the lower temperature thresholds of 121 species of stored product and food industry pests from six arthropod taxa (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera, and Blattodea). In particular, this review collected and summarized information regarding the lower development thresholds, lower population thresholds, lower acoustic or respiratory thresholds, lower walking and flying thresholds and lower trap capture thresholds for flying and walking arthropods. The average lower development threshold (LDT) differed among orders: the lowest was reported for Acari (6.8 °C) and Diptera (8.1 °C), followed by Lepidoptera (11.3 °C) and Psocoptera (13.8 °C), and the highest was reported for Coleoptera (14 °C) and Blattodea (15 °C). An exclusion-function was established showing the percentage of pest species (n = 112) that were developmentally suppressed (excluded) due to temperatures reaching the LDT in the range of decreasing temperatures from 25 °C to 0 °C. We scaled various temperature thresholds from the lowest to highest temperature as follows: the walking threshold, the trap capture threshold for walking insects, the lower development threshold, lower population threshold, lower flying threshold and the lower trap capture threshold for flying pests. Important pest species were identified for which information regarding the lower temperature threshold is missing, or for which the information is too variable and should be refined in future research.
Vaclav Stejskal; Tomas Vendl; Zhihong Li; Radek Aulicky; Zhiong Li. Minimal Thermal Requirements for Development and Activity of Stored Product and Food Industry Pests (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera and Blattodea): A Review. Insects 2019, 10, 149 .
AMA StyleVaclav Stejskal, Tomas Vendl, Zhihong Li, Radek Aulicky, Zhiong Li. Minimal Thermal Requirements for Development and Activity of Stored Product and Food Industry Pests (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera and Blattodea): A Review. Insects. 2019; 10 (5):149.
Chicago/Turabian StyleVaclav Stejskal; Tomas Vendl; Zhihong Li; Radek Aulicky; Zhiong Li. 2019. "Minimal Thermal Requirements for Development and Activity of Stored Product and Food Industry Pests (Acari, Coleoptera, Lepidoptera, Psocoptera, Diptera and Blattodea): A Review." Insects 10, no. 5: 149.
Booklice in the genus Liposcelis (Psocodea: Liposcelididae) are essential storage pests worldwide. Fragmented mt genomes have been identified in the Liposcelis species together with the typical mitochondrial (mt) genome, which is a single circular chromosome with 37 genes. Gene rearrangement, pseudogenes, and repeat regions (RRs) are very common among fragmented mt genomes. We sequenced the mt genome of the booklouse L. brunnea, the type species of the genus Liposcelis. We identified 37 genes in the mt genome of L. brunnea, which was fragmented into three chromosomes. The chromosomes I, II, III were 7.3 kb, 5.5 kb, and 5.3 kb in size with 9, 19, and 15 genes, respectively. In addition, 16 pseudogenes and four repeat regions were present in three chromosomes. Gene rearrangement in the mt genome of L. brunnea was obvious compared to that in other mt genomes in the genus Liposcelis. We found a possible correlation among mt genome rearrangement, the morphological classification standard, and phylogenetic relationships. In summary, a three-chromosome mt genome in an insect was identified for the first time, which may aid in understanding mt genome fragmentation, gene rearrangement, and evolution.
Shiqian Feng; Hu Li; Fan Song; Yannan Wang; Vaclav Stejskal; Wanzhi Cai; Zhihong Li. A novel mitochondrial genome fragmentation pattern in Liposcelis brunnea, the type species of the genus Liposcelis (Psocodea: Liposcelididae). International Journal of Biological Macromolecules 2019, 132, 1296 -1303.
AMA StyleShiqian Feng, Hu Li, Fan Song, Yannan Wang, Vaclav Stejskal, Wanzhi Cai, Zhihong Li. A novel mitochondrial genome fragmentation pattern in Liposcelis brunnea, the type species of the genus Liposcelis (Psocodea: Liposcelididae). International Journal of Biological Macromolecules. 2019; 132 ():1296-1303.
Chicago/Turabian StyleShiqian Feng; Hu Li; Fan Song; Yannan Wang; Vaclav Stejskal; Wanzhi Cai; Zhihong Li. 2019. "A novel mitochondrial genome fragmentation pattern in Liposcelis brunnea, the type species of the genus Liposcelis (Psocodea: Liposcelididae)." International Journal of Biological Macromolecules 132, no. : 1296-1303.
This study first estimated the current state of phosphine sensitivity (using a knock-down/KT100/Degesch kit) in Sitophilus granarius (23 strains) and Tribolium castaneum (8 strains) in Czech Republic grain stores. The resistance of S. granarius (21.7% resistant strains; coefficient of resistance KT100 ranged from 0.5 to 2.3 among strains) was substantially lower and less frequent than that of T. castaneum (87.5% resistant strains; coefficient of resistance KT100 ranged from 0.9 to 52.5 among strains). The phosphine efficacy of the laboratory and field (i.e., resistant) pest strains was validated during commercial fumigation when suboptimal tarpaulin sealing resulted in low-concentration phosphine exposure (Ct products ranged from 5.9 to 7.4 g*hr/m3). Although even low-dose fumigation led to 100% adult mortality of both laboratory and field strains of S. granarius and laboratory strains of T. castaneum, the mortality of the field strain of T. castaneum ranged from 47% to 95%. Larval emergence from the fumigated commodity samples with pest eggs was zero or near zero for laboratory strains, while 1.3–6.0 (S. granarius) and 63.7–80.00 (T. castaneum) field-strain larvae emerged per sample (100 g). This study shows that although a high proportion of the tested pest populations were still sensitive, several T. castaneum populations showed an elevated level of resistance that may decrease field fumigation efficacy, especially under suboptimal phosphine dosage conditions.
Radek Aulicky; Vaclav Stejskal; Barbora Frydova. Field validation of phosphine efficacy on the first recorded resistant strains of Sitophilus granarius and Tribolium castaneum from the Czech Republic. Journal of Stored Products Research 2019, 81, 107 -113.
AMA StyleRadek Aulicky, Vaclav Stejskal, Barbora Frydova. Field validation of phosphine efficacy on the first recorded resistant strains of Sitophilus granarius and Tribolium castaneum from the Czech Republic. Journal of Stored Products Research. 2019; 81 ():107-113.
Chicago/Turabian StyleRadek Aulicky; Vaclav Stejskal; Barbora Frydova. 2019. "Field validation of phosphine efficacy on the first recorded resistant strains of Sitophilus granarius and Tribolium castaneum from the Czech Republic." Journal of Stored Products Research 81, no. : 107-113.
In the laboratory, we evaluated the efficacy of the use of fluorescent non-toxic bait for the monitoring of wild roof rats (Rattus rattus). We described the temporal dynamics of the production of fluorescent faeces after the consumption of fluorescent bait and compared it with those of R. norvegicus and Mus musculus. Roof rats produced, on average, 52 faecal pellets per 24 h, ranging from 31 to 81. The dry weight of the produced faeces per 24 h ranged from 1.1 to 3.4 g. The production peak of highly detectable fluorescent faeces was 8–18 h after consuming the bait, and the last detectable faeces were recorded 28–30 h after consuming the bait. Our data showed that roof rats produced the highest proportion of highly fluorescent faeces, indicating that best physiological potential for the use of this type of fluorescent bait exists in R. rattus since R. norvegicus and M. musculus produced 80% and 65% of the fluorescent faeces produced by R. rattus, respectively.
Marcela Frankova; Barbora Kaftanova; Radek Aulicky; Pavel Rodl; Daniel Frynta; Vaclav Stejskal. Temporal production of coloured faeces in wild roof rats (Rattus rattus) following consumption of fluorescent non-toxic bait and a comparison with wild R. norvegicus and Mus musculus. Journal of Stored Products Research 2018, 81, 7 -10.
AMA StyleMarcela Frankova, Barbora Kaftanova, Radek Aulicky, Pavel Rodl, Daniel Frynta, Vaclav Stejskal. Temporal production of coloured faeces in wild roof rats (Rattus rattus) following consumption of fluorescent non-toxic bait and a comparison with wild R. norvegicus and Mus musculus. Journal of Stored Products Research. 2018; 81 ():7-10.
Chicago/Turabian StyleMarcela Frankova; Barbora Kaftanova; Radek Aulicky; Pavel Rodl; Daniel Frynta; Vaclav Stejskal. 2018. "Temporal production of coloured faeces in wild roof rats (Rattus rattus) following consumption of fluorescent non-toxic bait and a comparison with wild R. norvegicus and Mus musculus." Journal of Stored Products Research 81, no. : 7-10.
Although it is known that separate insect body structures may be asymmetrical within one species, the different functional asymmetries within a single organ as a result of differential selective regimes have not been described. Based on microscopic measurements and SEM photography, we examined the size, shape and asymmetry of the mandibular structures of males and females of the sexually dimorphic broad-horned flour beetle, Gnatocerus cornutus (Tenebrionidae, Coleoptera). It was found that sexual dimorphism only manifests in certain outgrowth parts (horns) of male mandibles, while the remaining cutting parts of the mandibles hold identical morphologies for both sexes. A more interesting finding—since this is the first published case of dual functionally selected asymmetry in an identical arthropod organ—was that the cutting part of the male mandible exhibited directional asymmetry, whereas the outgrowth horn part of the mandible showed a high degree of symmetry. Moreover, there was no relationship between the size and asymmetry of horns. The results indicate different regulatory mechanisms of sexually selected combative horns and the food-functional, more conservative (constrained by hard food and adult long life) cutting parts of mandibles.
Tomas Vendl; Vaclav Stejskal; Radek Aulicky. First Case of Dual Size Asymmetry in an Identical Arthropod Organ: Different Asymmetries of the Combative (Sexual) and Cutting (Non-Sexual) Parts of Mandibles in the Horned Stored-Product Beetle Gnatocerus cornutus (Fabricius, 1798). Insects 2018, 9, 151 .
AMA StyleTomas Vendl, Vaclav Stejskal, Radek Aulicky. First Case of Dual Size Asymmetry in an Identical Arthropod Organ: Different Asymmetries of the Combative (Sexual) and Cutting (Non-Sexual) Parts of Mandibles in the Horned Stored-Product Beetle Gnatocerus cornutus (Fabricius, 1798). Insects. 2018; 9 (4):151.
Chicago/Turabian StyleTomas Vendl; Vaclav Stejskal; Radek Aulicky. 2018. "First Case of Dual Size Asymmetry in an Identical Arthropod Organ: Different Asymmetries of the Combative (Sexual) and Cutting (Non-Sexual) Parts of Mandibles in the Horned Stored-Product Beetle Gnatocerus cornutus (Fabricius, 1798)." Insects 9, no. 4: 151.
The order Psocodea which has incorporated the two former orders Psocoptera (barklice and booklice) and Phthiraptera (parasitic lice) attracts much attention for its unusual mitochondrial (mt) genome rearrangements. Available phylogenetic analysis for Psocodea is subjected to partial taxa and a complete one is needed. To further explore the genome rearrangement and phylogeny in Psocodea, we sequenced the mt genomes of two barklice, Lepinotus reticulatus (collected from China) and Dorypteryx domestica (collected from Czech Republic). Both of newly sequenced barklice had typical one-chromosome mt genomes and the same mt gene arrangement with the reported Lepidopsocidae sp. The mt genomes of L. reticulatus and D. domestica contained 37 genes typical of bilateral animals. In contrast with the recent report mt genome of D. domestica, our strain was found with many single nucleotide polymorphisms in intra-specific difference. Phylogenetic relationships were inferred from all available mt genomes of Psocodea data using Maximum Likelihood and Bayesian methods. The mt genome of L. reticulatus is the first representative with complete sequences of the family Trogiidae and our D. domestica data enriched the family Psyllipsocidae, which will contribute to the further study of mt gene rearrangement and phylogeny of Psocodea.
Shiqian Feng; Václav Stejskal; Yannan Wang; Zhihong Li. The mitochondrial genomes of the barklice, Lepinotus reticulatus and Dorypteryx domestica (Psocodea: Trogiomorpha): Insight into phylogeny of the order Psocodea. International Journal of Biological Macromolecules 2018, 116, 247 -254.
AMA StyleShiqian Feng, Václav Stejskal, Yannan Wang, Zhihong Li. The mitochondrial genomes of the barklice, Lepinotus reticulatus and Dorypteryx domestica (Psocodea: Trogiomorpha): Insight into phylogeny of the order Psocodea. International Journal of Biological Macromolecules. 2018; 116 ():247-254.
Chicago/Turabian StyleShiqian Feng; Václav Stejskal; Yannan Wang; Zhihong Li. 2018. "The mitochondrial genomes of the barklice, Lepinotus reticulatus and Dorypteryx domestica (Psocodea: Trogiomorpha): Insight into phylogeny of the order Psocodea." International Journal of Biological Macromolecules 116, no. : 247-254.
Vaclav Stejskal; Jan Hubert; Zhihong Li. Erratum to: Human Health Problems and Accidents Associated with Occurrence and Control of Storage Arthropods and Rodents. Recent Advances in Stored Product Protection 2018, E1 -E1.
AMA StyleVaclav Stejskal, Jan Hubert, Zhihong Li. Erratum to: Human Health Problems and Accidents Associated with Occurrence and Control of Storage Arthropods and Rodents. Recent Advances in Stored Product Protection. 2018; ():E1-E1.
Chicago/Turabian StyleVaclav Stejskal; Jan Hubert; Zhihong Li. 2018. "Erratum to: Human Health Problems and Accidents Associated with Occurrence and Control of Storage Arthropods and Rodents." Recent Advances in Stored Product Protection , no. : E1-E1.
The UN Food and Agriculture Organization (FAO) estimates that the global human population will reach 9.8 billion in the year 2050, that is over 70% higher than the previous estimate cited by Hagstrum and Phillips (Ann Rev Entomol 62:379–397, 2017).
Vaclav Stejskal; Jan Hubert; Zhihong Li. Human Health Problems and Accidents Associated with Occurrence and Control of Storage Arthropods and Rodents. Recent Advances in Stored Product Protection 2018, 19 -43.
AMA StyleVaclav Stejskal, Jan Hubert, Zhihong Li. Human Health Problems and Accidents Associated with Occurrence and Control of Storage Arthropods and Rodents. Recent Advances in Stored Product Protection. 2018; ():19-43.
Chicago/Turabian StyleVaclav Stejskal; Jan Hubert; Zhihong Li. 2018. "Human Health Problems and Accidents Associated with Occurrence and Control of Storage Arthropods and Rodents." Recent Advances in Stored Product Protection , no. : 19-43.
Insects and mites are common inhabitants and accidental invaders of food, including durable commodities, and their presence can have both direct and indirect effects on human health. The most common direct effect is contamination of food with arthropod fragments and related contaminants, which may be allergenic or even carcinogenic. The most important indirect effect is that their presence can change the storage microenvironment, making durable products suitable for the rapid development of fungi and other microorganisms. Some of these fungi can produce toxins (e.g., aflatoxins) that endanger human health. Insects may actively or passively contribute to the spread of microorganisms, increasing product contamination, and they may host bacteria that have developed antibiotic resistance, contributing to their spread in food. Several species also may host, attract, or transmit tapeworms, predators, or parasitoids that may affect health. This review synthesizes research on these topics and suggests directions for future research.
Jan Hubert; Vaclav Stejskal; Christos G. Athanassiou; James E. Throne. Health Hazards Associated with Arthropod Infestation of Stored Products. Annual Review of Entomology 2018, 63, 553 -573.
AMA StyleJan Hubert, Vaclav Stejskal, Christos G. Athanassiou, James E. Throne. Health Hazards Associated with Arthropod Infestation of Stored Products. Annual Review of Entomology. 2018; 63 (1):553-573.
Chicago/Turabian StyleJan Hubert; Vaclav Stejskal; Christos G. Athanassiou; James E. Throne. 2018. "Health Hazards Associated with Arthropod Infestation of Stored Products." Annual Review of Entomology 63, no. 1: 553-573.
Marcela Frankova; Vaclav Stejskal; Radek Aulicky. Suppression of food intake by house mouse ( Mus musculus ) following ingestion of brodifacoum-based rodenticide bait. Crop Protection 2017, 100, 134 -137.
AMA StyleMarcela Frankova, Vaclav Stejskal, Radek Aulicky. Suppression of food intake by house mouse ( Mus musculus ) following ingestion of brodifacoum-based rodenticide bait. Crop Protection. 2017; 100 ():134-137.
Chicago/Turabian StyleMarcela Frankova; Vaclav Stejskal; Radek Aulicky. 2017. "Suppression of food intake by house mouse ( Mus musculus ) following ingestion of brodifacoum-based rodenticide bait." Crop Protection 100, no. : 134-137.
Vaclav Stejskal; Marie Bostlova; Marta Nesvorna; Vlado Volek; Vaclav Dolezal; Jan Hubert. Comparison of the resistance of mono- and multilayer packaging films to stored-product insects in a laboratory test. Food Control 2017, 73, 566 -573.
AMA StyleVaclav Stejskal, Marie Bostlova, Marta Nesvorna, Vlado Volek, Vaclav Dolezal, Jan Hubert. Comparison of the resistance of mono- and multilayer packaging films to stored-product insects in a laboratory test. Food Control. 2017; 73 ():566-573.
Chicago/Turabian StyleVaclav Stejskal; Marie Bostlova; Marta Nesvorna; Vlado Volek; Vaclav Dolezal; Jan Hubert. 2017. "Comparison of the resistance of mono- and multilayer packaging films to stored-product insects in a laboratory test." Food Control 73, no. : 566-573.
Cheyletus malaccensis Oudemans and Cheyletus eruditus (Schrank) are two sibling species of the Cheyletus genus. Both species are beneficial predators of stored product pests. It is difficult to distinguish them based only on morphological characteristics. In this study, we describe species-specific PCR for molecular identification of C. malaccensis and C. eruditus. Species-specific primer pairs were designed on the basis of variations in the mtDNA COI barcode sequences. A 403 bp-specific fragment of C. malaccensis and a 421 bp absolute product of C. eruditus were generated by their respective primer pairs, while no such diagnostic bands were present in any of the nine pest mite species tested. The results of the sensitivity test demonstrated that the detection limit of DNA template concentration was 1 ng/μL for both C. malaccensis and C. eruditus. This study exhibited high specificity, reliability, rapid and low cost for mite specie diagnosis. Our data show that species-specific PCR is a powerful tool that can be used for identification of C. malaccensis and C. eruditus.
Yi Wu; Fujun Li; Zhihong Li; Václav Stejskal; Radek Aulicky; Zuzana Kučerová; Tao Zhang; Peihuan He; Yang Cao. Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR. Journal of Stored Products Research 2016, 69, 213 -216.
AMA StyleYi Wu, Fujun Li, Zhihong Li, Václav Stejskal, Radek Aulicky, Zuzana Kučerová, Tao Zhang, Peihuan He, Yang Cao. Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR. Journal of Stored Products Research. 2016; 69 ():213-216.
Chicago/Turabian StyleYi Wu; Fujun Li; Zhihong Li; Václav Stejskal; Radek Aulicky; Zuzana Kučerová; Tao Zhang; Peihuan He; Yang Cao. 2016. "Rapid diagnosis of two common stored-product predatory mite species based on species-specific PCR." Journal of Stored Products Research 69, no. : 213-216.
Radek Aulický; Vaclav Stejskal; Milan Dlouhy; Jana Liskova. Validation of hydrogen cyanide fumigation in flourmills to control the confused flour beetle. Czech Journal of Food Sciences 2016, 33, 174 -179.
AMA StyleRadek Aulický, Vaclav Stejskal, Milan Dlouhy, Jana Liskova. Validation of hydrogen cyanide fumigation in flourmills to control the confused flour beetle. Czech Journal of Food Sciences. 2016; 33 (2):174-179.
Chicago/Turabian StyleRadek Aulický; Vaclav Stejskal; Milan Dlouhy; Jana Liskova. 2016. "Validation of hydrogen cyanide fumigation in flourmills to control the confused flour beetle." Czech Journal of Food Sciences 33, no. 2: 174-179.