This page has only limited features, please log in for full access.
Contamination of surface waters with pathogens as well as all diseases associated with such events are a significant concern worldwide. In recent decades, there has been a growing interest in developing analytical methods with good performance for the detection of this category of contaminants. The most important analytical methods applied for the determination of bacteria in waters are traditional ones (such as bacterial culturing methods, enzyme-linked immunoassay, polymerase chain reaction, and loop-mediated isothermal amplification) and advanced alternative methods (such as spectrometry, chromatography, capillary electrophoresis, surface-enhanced Raman scattering, and magnetic field-assisted and hyphenated techniques). In addition, optical and electrochemical sensors have gained much attention as essential alternatives for the conventional detection of bacteria. The large number of available methods have been materialized by many publications in this field aimed to ensure the control of water quality in water resources. This study represents a critical synthesis of the literature regarding the latest analytical methods covering comparative aspects of pathogen contamination of water resources. All these aspects are presented as representative examples, focusing on two important bacteria with essential implications on the health of the population, namely Pseudomonas aeruginosa and Escherichia coli.
Alexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability 2021, 13, 7229 .
AMA StyleAlexandra Canciu, Mihaela Tertis, Oana Hosu, Andreea Cernat, Cecilia Cristea, Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability. 2021; 13 (13):7229.
Chicago/Turabian StyleAlexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. 2021. "Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters." Sustainability 13, no. 13: 7229.
Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.
Mihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules 2021, 26, 3200 .
AMA StyleMihaela Tertis, Oana Hosu, Bogdan Feier, Andreea Cernat, Anca Florea, Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules. 2021; 26 (11):3200.
Chicago/Turabian StyleMihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. 2021. "Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection." Molecules 26, no. 11: 3200.
Aptamers have come in the spotlight as bio-mimetic molecular recognition elements in the field of biomedicine due to various applications in diagnostics, drug delivery, therapeutics, and pharmaceutical analysis. Aptamers are composed of nucleic acid strands (DNA or RNA) that can specifically interact in a three-dimensional tailored design with the target molecule. The basic method to generate aptamers is Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Recent technological advances in aptamer selection allow for faster and cheaper production of a new generation of high-affinity aptamers compared to the traditional SELEX, which can last up to several months. Rigorous characterization performed by multiple research groups endorsed several well-defined aptamer sequences. Binding affinity, nature of the biomolecular interactions and structural characterization are of paramount importance for aptamer screening and development of applications. However, remarkable challenges still need to be dealt with before the aptamers can make great contributions to the biomedical field. Poor specificity and sensitivity, questionable clinical use, low drug loading, in vivo stability and toxicity are only some of the identified challenges. This review accounts for the 30th celebration of the SELEX technology underlining the most important aptamers’ achievements in the biomedical field within mostly the past five years. Aptamers’ advantages over antibodies are discussed. Because of potential clinical translational utility, insights of remarkable developments in aptamer-based methods for diagnosis and monitoring of disease biomarkers and pharmaceuticals are discussed focusing on the recent studies (2015–2020). The current challenges and promising opportunities for aptamers for therapeutic and theragnostic purposes are also presented.
Geanina Ștefan; Oana Hosu; Karolien De Wael; María Jesús Lobo-Castañón; Cecilia Cristea. Aptamers in biomedicine: Selection strategies and recent advances. Electrochimica Acta 2021, 376, 137994 .
AMA StyleGeanina Ștefan, Oana Hosu, Karolien De Wael, María Jesús Lobo-Castañón, Cecilia Cristea. Aptamers in biomedicine: Selection strategies and recent advances. Electrochimica Acta. 2021; 376 ():137994.
Chicago/Turabian StyleGeanina Ștefan; Oana Hosu; Karolien De Wael; María Jesús Lobo-Castañón; Cecilia Cristea. 2021. "Aptamers in biomedicine: Selection strategies and recent advances." Electrochimica Acta 376, no. : 137994.
A novel hybrid composite of conductive poly(methylene blue) (PMB) and carbon nanotubes (CNT) was prepared for the detection of 5-aminosalicylic acid (5-ASA). Electrosynthesis of PMB with glassy carbon electrode (GCE) or with carbon nanotube modified GCE was done in ethaline deep eutectic solvent of choline chloride mixed with ethylene glycol and a 10% v/v aqueous solution. Different sensor architectures were evaluated in a broad range of pH values in a Britton-Robinson (BR) buffer using electrochemical techniques, chronoamperometry (CA), and differential pulse voltammetry (DPV), to determine the optimum sensor configuration for 5-ASA sensing. Under optimal conditions, the best analytical performance was obtained with CNT/PMBDES/GCE in 0.04 M BR buffer pH 7.0 in the range 5–100 µM 5-ASA using the DPV method, with an excellent sensitivity of 9.84 μA cm−2 μM−1 (4.9 % RSD, n = 5) and a detection limit (LOD) (3σ/slope) of 7.7 nM, outclassing most similar sensors found in the literature. The sensitivity of the same sensor obtained in CA (1.33 μA cm−2 μM−1) under optimal conditions (pH 7.0, E app = +0.40 V) was lower than that obtained by DPV. Simultaneous detection of 5-ASA and its analogue, acetaminophen (APAP), was successfully realized, showing a catalytic effect towards the electro-oxidation of both analytes, lowering their oxidation overpotential, and enhancing the oxidation peak currents and peak-to-peak separation as compared with the unmodified electrode. The proposed method is simple, sensitive, easy to apply, and economical for routine analysis.
Oana Hosu; Madalina Barsan; Robert Săndulescu; Cecilia Cristea; Christopher Brett. Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid. Sensors 2021, 21, 1161 .
AMA StyleOana Hosu, Madalina Barsan, Robert Săndulescu, Cecilia Cristea, Christopher Brett. Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid. Sensors. 2021; 21 (4):1161.
Chicago/Turabian StyleOana Hosu; Madalina Barsan; Robert Săndulescu; Cecilia Cristea; Christopher Brett. 2021. "Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid." Sensors 21, no. 4: 1161.
During the last few decades, magnetic nanoparticles have been evaluated as promising materials in the field of cancer detection, screening, and treatment. Early diagnosis and screening of cancer may be achieved using magnetic nanoparticles either within the magnetic resonance imaging technique and/or sensing systems. These sensors are designed to selectively detect specific biomarkers, compounds that can be related to the onset or evolution of cancer, during and after the treatment of this widespread disease. Some of the particular properties of magnetic nanoparticles are extensively exploited in cancer therapy as drug delivery agents to selectively target the envisaged location by tailored in vivo manipulation using an external magnetic field. Furthermore, individualized treatment with antineoplastic drugs may be combined with magnetic resonance imaging to achieve an efficient therapy. This review summarizes the studies about the implications of magnetic nanoparticles in cancer diagnosis, treatment and drug delivery as well as prospects for future development and challenges of magnetic nanoparticles in the field of oncology.
Oana Hosu; Mihaela Tertis; Cecilia Cristea; Hosu. Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment. Magnetochemistry 2019, 5, 55 .
AMA StyleOana Hosu, Mihaela Tertis, Cecilia Cristea, Hosu. Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment. Magnetochemistry. 2019; 5 (4):55.
Chicago/Turabian StyleOana Hosu; Mihaela Tertis; Cecilia Cristea; Hosu. 2019. "Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment." Magnetochemistry 5, no. 4: 55.
Nowadays, foodborne allergies and illnesses have become a global menace on public health. Product mislabeling, accidental cross-contamination or intentional adulteration with low quality or unsafe ingredients for economic purposes could constitute a serious drawback: thus, safety and quality are of paramount importance in the food industry. Analytical strategies for on-site sensitive detection and screening of food allergens and food contaminants are becoming fundamental, since a huge development is being made in functional foods and new food manufacturing technologies. From the perspective of a quick and accurate analysis, immunosensors represent ideal tools that are able to provide a specific and fast response, since they are portable devices of low-cost and are easy to use. Among the different types of immunosensors, electrochemical immunosensors are widely accepted because of their large applicability such as screening, monitoring or diagnosing. This chapter summarizes a general overview of allergens, the applied nanotechnology and the fundamentals of immunosensors built to be applied in food analysis. The approaches used to develop electrochemical immunosensors for detecting foodborne allergens are emphasized.
G. Selvolini; Oana Hosu; G. Marrazza. CHAPTER 7. Immunosensors for Food Allergens: An Overview. Detection Science 2019, 135 -155.
AMA StyleG. Selvolini, Oana Hosu, G. Marrazza. CHAPTER 7. Immunosensors for Food Allergens: An Overview. Detection Science. 2019; ():135-155.
Chicago/Turabian StyleG. Selvolini; Oana Hosu; G. Marrazza. 2019. "CHAPTER 7. Immunosensors for Food Allergens: An Overview." Detection Science , no. : 135-155.
Mariagrazia Lettieri; Oana Hosu; Alina Adumitrachioaie; Cecilia Cristea; Giovanna Marrazza. Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer. Electroanalysis 2019, 32, 217 -225.
AMA StyleMariagrazia Lettieri, Oana Hosu, Alina Adumitrachioaie, Cecilia Cristea, Giovanna Marrazza. Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer. Electroanalysis. 2019; 32 (2):217-225.
Chicago/Turabian StyleMariagrazia Lettieri; Oana Hosu; Alina Adumitrachioaie; Cecilia Cristea; Giovanna Marrazza. 2019. "Beta‐lactoglobulin Electrochemical Detection Based with an Innovative Platform Based on Composite Polymer." Electroanalysis 32, no. 2: 217-225.
In this work, we present a smartphone-based multiplexed enzymatic biosensor utilizing the unique colorimetric properties of the poly(aniline-co-anthranilic acid) (ANI-co-AA) composite film coupled with horseradish peroxidase (HRP), glucose oxidase (GOx), horseradish peroxidase-glucose oxidase (GOx-HRP) and tyrosinase (Tyr) enzymes. The enzymes are immobilized on the composite polymer film by adsorption and they catalyze a reversible redox color change of the host polymer from green to blue in the presence of their substrate. A smartphone was applied as color detector, for image acquisition and data handling. A ColorLab® android application, free of charge software application, was used to enable easy and clear display of the sensors’ response indicating remarkable changes in the optical features. The results were confirmed by the spectrophotometric measurements. The developed colorimetric enzymatic biosensors were studied and optimized in relation to different experimental parameters. Moreover, the colorimetric enzymatic biosensors were applied to food and pharmaceutical analysis. It has been shown by these studies that the colorimetric biosensors are promising as quick and simple tests for handheld analysis in various fields.
Oana Hosu; Mariagrazia Lettieri; Nicoleta Papara; Andrea Ravalli; Robert Sandulescu; Cecilia Cristea; Giovanna Marrazza. Colorimetric multienzymatic smart sensors for hydrogen peroxide, glucose and catechol screening analysis. Talanta 2019, 204, 525 -532.
AMA StyleOana Hosu, Mariagrazia Lettieri, Nicoleta Papara, Andrea Ravalli, Robert Sandulescu, Cecilia Cristea, Giovanna Marrazza. Colorimetric multienzymatic smart sensors for hydrogen peroxide, glucose and catechol screening analysis. Talanta. 2019; 204 ():525-532.
Chicago/Turabian StyleOana Hosu; Mariagrazia Lettieri; Nicoleta Papara; Andrea Ravalli; Robert Sandulescu; Cecilia Cristea; Giovanna Marrazza. 2019. "Colorimetric multienzymatic smart sensors for hydrogen peroxide, glucose and catechol screening analysis." Talanta 204, no. : 525-532.
Arsenic, one of the most abundant mineral and also one to the most toxic compounds. Due to its high toxicity sensitive analytical methods are highly important, taking into account that the admitted level is in the range of µg L−1. A novel and easy to use platform for As(III) detection from water samples is proposed, based on gold and platinum bi metallic nanoparticles and a conductive polymer (polyaniline). The electrochemical detection was achieved after optimization of cathodic pre-concentration and stripping parameters by square wave anodic stripping voltammetry at modified screen-printed carbon-based electrochemical cells, proving its applicability for disposable and cost-effective in situ analysis of arsenic.
Gheorghe Melinte; Oana Hosu; Mariagrazia Lettieri; Cecilia Cristea; Giovanna Marrazza. Electrochemical Fingerprint of Arsenic (III) by Using Hybrid Nanocomposite-Based Platforms. Sensors 2019, 19, 2279 .
AMA StyleGheorghe Melinte, Oana Hosu, Mariagrazia Lettieri, Cecilia Cristea, Giovanna Marrazza. Electrochemical Fingerprint of Arsenic (III) by Using Hybrid Nanocomposite-Based Platforms. Sensors. 2019; 19 (10):2279.
Chicago/Turabian StyleGheorghe Melinte; Oana Hosu; Mariagrazia Lettieri; Cecilia Cristea; Giovanna Marrazza. 2019. "Electrochemical Fingerprint of Arsenic (III) by Using Hybrid Nanocomposite-Based Platforms." Sensors 19, no. 10: 2279.
Neurotransmitters are endogenous chemical messengers which play an important role in many of the brain functions, abnormal levels being correlated with physical, psychotic and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Therefore, their sensitive and robust detection is of great clinical significance. Electrochemical methods have been intensively used in the last decades for neurotransmitter detection, outclassing more complicated analytical techniques such as conventional spectrophotometry, chromatography, fluorescence, flow injection, and capillary electrophoresis. In this manuscript, the most successful and promising electrochemical enzyme-free and enzymatic sensors for neurotransmitter detection are reviewed. Focusing on the activity of worldwide researchers mainly during the last ten years (2010–2019), without pretending to be exhaustive, we present an overview of the progress made in sensing strategies during this time. Particular emphasis is placed on nanostructured-based sensors, which show a substantial improvement of the analytical performances. This review also examines the progress made in biosensors for neurotransmitter measurements in vitro, in vivo and ex vivo.
Tavakolian- Ardakani; Oana Hosu; Cecilia Cristea; Mazloum- Ardakani; Giovanna Marrazza. Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review. Sensors 2019, 19, 2037 .
AMA StyleTavakolian- Ardakani, Oana Hosu, Cecilia Cristea, Mazloum- Ardakani, Giovanna Marrazza. Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review. Sensors. 2019; 19 (9):2037.
Chicago/Turabian StyleTavakolian- Ardakani; Oana Hosu; Cecilia Cristea; Mazloum- Ardakani; Giovanna Marrazza. 2019. "Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review." Sensors 19, no. 9: 2037.
Saliva is a complex matrix that reflects hormonal, immunological, metabolic and nutritional state of a person. Moreover, it is considered the most appropriate biological fluid for scientific investigations regarding ethics. Saliva chemistry is an emerging field and mostly employed in toxicology and forensic medicine for analysing and detecting user drug addiction and alcohol abuse by mapping the blood and saliva data. The advantages of salivary testing include helping in the diagnosis, prognosis and surveillance of disease, alongside with the non-invasive and ease of collection properties. The need for fast, cost-effective and reliable methods for saliva investigation has led to high technological advances in developing sensing tools. Hence, increasing number of papers are reporting diseases signalling salivary biomarkers for local or systemic pathologies like oral cancer, endocrine and immunologic pathologies, infectious and neurological diseases etc. This review focuses on the recent advances and main challenges employed in the detection and quantification of salivary biomarkers by separation, immunological and electrochemical techniques and highlight the future perspectives of the point-of-care devices in diagnosing local or systemic pathologies.
Ioana Tiuca Gug; Mihaela Tertis; Oana Hosu; Cecilia Cristea. Salivary biomarkers detection: Analytical and immunological methods overview. TrAC Trends in Analytical Chemistry 2019, 113, 301 -316.
AMA StyleIoana Tiuca Gug, Mihaela Tertis, Oana Hosu, Cecilia Cristea. Salivary biomarkers detection: Analytical and immunological methods overview. TrAC Trends in Analytical Chemistry. 2019; 113 ():301-316.
Chicago/Turabian StyleIoana Tiuca Gug; Mihaela Tertis; Oana Hosu; Cecilia Cristea. 2019. "Salivary biomarkers detection: Analytical and immunological methods overview." TrAC Trends in Analytical Chemistry 113, no. : 301-316.
In this work, we propose an electrochemical DNA-based sensor for sensitive detection of organophosphorus pesticides. To improve the sensitivity of the DNA-based sensor, polyaniline film and gold nanoparticles were progressively electrodeposited on the graphite screen-printed electrode surface by cyclic voltammetry. Gold nanoparticles were then employed as platform for the immobilization of thiol-tethered DNA oligonucleotide sequence complementary to the selected biotinylated DNA aptamer for profenofos detection. Streptavidin-alkaline phosphatase enzyme conjugate was then added to trace the affinity reaction through the hydrolysis of 1-naphthyl phosphate to 1-naphthol, which was then detected by differential pulse voltammetry. A decrease of the signal was obtained when the pesticide concentration was increased, making the sensor work as signal off sensor.
Giulia Selvolini; Ioana Băjan; Oana Hosu; Cecilia Cristea; Robert Săndulescu; Giovanna Marrazza. Electrochemical DNA-Based Sensor for Organophosphorus Pesticides Detection. Lecture Notes in Electrical Engineering 2019, 111 -115.
AMA StyleGiulia Selvolini, Ioana Băjan, Oana Hosu, Cecilia Cristea, Robert Săndulescu, Giovanna Marrazza. Electrochemical DNA-Based Sensor for Organophosphorus Pesticides Detection. Lecture Notes in Electrical Engineering. 2019; ():111-115.
Chicago/Turabian StyleGiulia Selvolini; Ioana Băjan; Oana Hosu; Cecilia Cristea; Robert Săndulescu; Giovanna Marrazza. 2019. "Electrochemical DNA-Based Sensor for Organophosphorus Pesticides Detection." Lecture Notes in Electrical Engineering , no. : 111-115.
Background: The detection of biological molecules referred as biomarkers in biological fluids is fundamental in clinical analysis because it permits to discriminate between healthy and ill individuals and to evaluate the progress of a disease. The development of immunosensors for the detection and monitoring of biomarkers is currently a major area of research and, as more markers are discovered and their role in disease becomes better understood, this will continue to grow. Methods: We report the research progresses of electrochemical immunosensor applied in clinical analysis that have been published in the last three years. Results: The emphasis of this review is on the advances of the electrochemical immunosensors for detection and monitoring of cancerous, cardiovascular and neurological diseases. An immunosensor overview was presented as well as the biomarkers and biosensing systems currently used to detect the onset and monitor the progression of the mentioned diseases. Conclusions: Electrochemical biosensors focusing on a vast repertoire of analytes are now becoming one of the most widely explored scientific fields. This is due to their enormous potential in clinical diagnosis and biological process monitoring. In the near future, with the development of transducer technology, nano-sized material technology, and biomolecules engineering technology, biosensors should be powerful tools in several analytical areas.
Oana Hosu; Giulia Selvolini; Cecilia Cristea; Giovanna Marrazza. Electrochemical Immunosensors for Disease Detection and Diagnosis. Current Medicinal Chemistry 2018, 25, 4119 -4137.
AMA StyleOana Hosu, Giulia Selvolini, Cecilia Cristea, Giovanna Marrazza. Electrochemical Immunosensors for Disease Detection and Diagnosis. Current Medicinal Chemistry. 2018; 25 (33):4119-4137.
Chicago/Turabian StyleOana Hosu; Giulia Selvolini; Cecilia Cristea; Giovanna Marrazza. 2018. "Electrochemical Immunosensors for Disease Detection and Diagnosis." Current Medicinal Chemistry 25, no. 33: 4119-4137.
Sensitive detection of allergens in a large variety of food samples has become increasingly important considering the emergence of functional foods and new food manufacturing technologies. Safety and quality are key issues of today's food industry. In this review, we highlight the success achieved in the design of electrochemical immunosensors towards detection of protein allergens. Different methodologies employed for immobilization of bioreceptors on transducer surface and strategies for signal amplification will be discussed.
Oana Hosu; Giulia Selvolini; Giovanna Marrazza. Recent advances of immunosensors for detecting food allergens. Current Opinion in Electrochemistry 2018, 10, 149 -156.
AMA StyleOana Hosu, Giulia Selvolini, Giovanna Marrazza. Recent advances of immunosensors for detecting food allergens. Current Opinion in Electrochemistry. 2018; 10 ():149-156.
Chicago/Turabian StyleOana Hosu; Giulia Selvolini; Giovanna Marrazza. 2018. "Recent advances of immunosensors for detecting food allergens." Current Opinion in Electrochemistry 10, no. : 149-156.
In this work, we propose an electrochemical DNA aptasensor for the detection of profenofos, an organophosphorus pesticide, based on a competitive format and disposable graphite screen-printed electrodes (GSPEs). A thiol-tethered DNA capture probe, which results to be complementary to the chosen aptamer sequence, was immobilised on gold nanoparticles/polyaniline composite film-modified electrodes (AuNPs/PANI/GSPE). Different profenofos solutions containing a fixed amount of the biotinylated DNA aptamer were dropped onto the realized aptasensors. The hybridisation reaction was measured using a streptavidin-alkaline phosphatase enzyme conjugate, which catalyses the hydrolysis of 1-naphthyl -phosphate. The 1-naphtol enzymatic product was detected by means of differential pulse voltammetry (DPV). The aptasensor showed itself to work as a signal off sensor, according to the competitive format used. A dose response curve was obtained between 0.10 μM and 10 μM with a detection limit of 0.27 μM.
Giulia Selvolini; Ioana Băjan; Oana Hosu; Cecilia Cristea; Robert Săndulescu; Giovanna Marrazza. DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos. Sensors 2018, 18, 2035 .
AMA StyleGiulia Selvolini, Ioana Băjan, Oana Hosu, Cecilia Cristea, Robert Săndulescu, Giovanna Marrazza. DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos. Sensors. 2018; 18 (7):2035.
Chicago/Turabian StyleGiulia Selvolini; Ioana Băjan; Oana Hosu; Cecilia Cristea; Robert Săndulescu; Giovanna Marrazza. 2018. "DNA-Based Sensor for the Detection of an Organophosphorus Pesticide: Profenofos." Sensors 18, no. 7: 2035.
Advances in nanotechnologies have led to the development of nanoscale biosensors with exquisite sensitivity and versatility as their ultimate goal is to detect any biochemical and biophysical signals associated with a specific disease at the level of a single molecule or cell. The ability to detect disease-associated biomolecules is essential not only for disease diagnostics in the clinical settings but also for biomedical research involving drug discovery and development. Portable, faster, and low-cost devices are highly preferred for replacing time-consuming centralized laboratory analysis. The reduction in sensor size provides great versatility, but extreme miniaturization while keeping intact the selectivity and sensitivity characteristics to biosensors must be achieved. The sensitivity of biosensors can be increased using different nanomaterials like carbon nanotubes, graphene, and nanoparticles while the selectivity is gained when biomolecules are involved (enzymes, antibodies, aptamers). Several examples of tattoo, microneedle, and smartphone-based sensors as screening, diagnosing, and monitoring tools, representing the latest approaches for minimal invasive devices in this dynamic field of research, are presented.
Oana Hosu; Simona Mirel; Robert Săndulescu; Cecilia Cristea. Minireview: Smart tattoo, Microneedle, Point-Of-care, and Phone-Based Biosensors for Medical Screening, Diagnosis, and Monitoring. Analytical Letters 2018, 52, 78 -92.
AMA StyleOana Hosu, Simona Mirel, Robert Săndulescu, Cecilia Cristea. Minireview: Smart tattoo, Microneedle, Point-Of-care, and Phone-Based Biosensors for Medical Screening, Diagnosis, and Monitoring. Analytical Letters. 2018; 52 (1):78-92.
Chicago/Turabian StyleOana Hosu; Simona Mirel; Robert Săndulescu; Cecilia Cristea. 2018. "Minireview: Smart tattoo, Microneedle, Point-Of-care, and Phone-Based Biosensors for Medical Screening, Diagnosis, and Monitoring." Analytical Letters 52, no. 1: 78-92.
The redox polymer poly(methylene blue) was synthesized by electropolymerization in ethaline deep eutectic solvent (PMBDES), and employed in a new nanostructured composite together with carbon nanotubes (CNT). The polymer was formed either before or after CNT deposition on glassy carbon electrodes (GCE) to obtain GCE/PMBDES/CNT and GCE/CNT/PMBDES modified electrodes, respectively. The morphology of the hybrid films was investigated by scanning electron microscopy, and their electrochemical performance by cyclic voltammetry and electrochemical impedance spectroscopy. The composite with the highest faradaic response and electronic conductivity was identified and applied as a platform for the determination of ascorbic acid by fixed potential amperometry at +0.2 V vs. Ag/AgCl, and acetaminophen by square wave voltammetry and differential pulse voltammetry at +0.67 V vs. Ag/AgCl. The limits of detection of the best sensor configurations are 1.7 μM for AA, and 1.6 μM for APAP, and respective electrochemical sensitivities are 2.2 μA cm-2 μM−1 and 68.7 μA cm-2μM-1. The applicability of the electrochemical sensors was demonstrated by the successful quantification of both these key analytes in complex pharmaceutical formulations.
Oana Hosu; Madalina M. Barsan; Cecilia Cristea; Robert Săndulescu; Christopher M. A. Brett. Nanocomposites based on carbon nanotubes and redox-active polymers synthesized in a deep eutectic solvent as a new electrochemical sensing platform. Mikrochimica Acta 2017, 184, 3919 -3927.
AMA StyleOana Hosu, Madalina M. Barsan, Cecilia Cristea, Robert Săndulescu, Christopher M. A. Brett. Nanocomposites based on carbon nanotubes and redox-active polymers synthesized in a deep eutectic solvent as a new electrochemical sensing platform. Mikrochimica Acta. 2017; 184 (10):3919-3927.
Chicago/Turabian StyleOana Hosu; Madalina M. Barsan; Cecilia Cristea; Robert Săndulescu; Christopher M. A. Brett. 2017. "Nanocomposites based on carbon nanotubes and redox-active polymers synthesized in a deep eutectic solvent as a new electrochemical sensing platform." Mikrochimica Acta 184, no. 10: 3919-3927.
Oana Hosu; Mihaela Tertiș; Gheorghe Melinte; Bogdan Feier; Robert Săndulescu; Cecilia Cristea. Mucin 4 detection with a label-free electrochemical immunosensor. Electrochemistry Communications 2017, 80, 39 -43.
AMA StyleOana Hosu, Mihaela Tertiș, Gheorghe Melinte, Bogdan Feier, Robert Săndulescu, Cecilia Cristea. Mucin 4 detection with a label-free electrochemical immunosensor. Electrochemistry Communications. 2017; 80 ():39-43.
Chicago/Turabian StyleOana Hosu; Mihaela Tertiș; Gheorghe Melinte; Bogdan Feier; Robert Săndulescu; Cecilia Cristea. 2017. "Mucin 4 detection with a label-free electrochemical immunosensor." Electrochemistry Communications 80, no. : 39-43.
An original versatile methodology for molecular grafting on different surfaces via the photoinduced formation of a covalent bond based on a diazirine group is reported. The synthesis and electrochemical behavior of a new diazirine derivative which acts as a molecular linking bridge bearing both a photoactivatable covalent binding group (diazirine) and a non-covalent binding group (pyrene) is described. The resulting pyrene-diazirine was electropolymerized onto a platinum electrode and under UV irradiation was successfully used to graft ferrocenemethanol and glucose oxidase (model small molecule and protein, respectively), conferring specific properties on the resulting materials. Furthermore, the immobilization of the diazirine derivative onto multi-walled carbon nanotubes (MWCNT) by π-stacking interaction or by electropolymerization onto both bare and MWCNT-coated platinum electrodes enabled photografting of glucose oxidase.
Oana Hosu; Kamal Elouarzaki; Karine Gorgy; Cecilia Cristea; Robert Sandulescu; Robert S. Marks; Serge Cosnier. Nanostructured photoactivatable electrode surface based on pyrene diazirine. Electrochemistry Communications 2017, 80, 5 -8.
AMA StyleOana Hosu, Kamal Elouarzaki, Karine Gorgy, Cecilia Cristea, Robert Sandulescu, Robert S. Marks, Serge Cosnier. Nanostructured photoactivatable electrode surface based on pyrene diazirine. Electrochemistry Communications. 2017; 80 ():5-8.
Chicago/Turabian StyleOana Hosu; Kamal Elouarzaki; Karine Gorgy; Cecilia Cristea; Robert Sandulescu; Robert S. Marks; Serge Cosnier. 2017. "Nanostructured photoactivatable electrode surface based on pyrene diazirine." Electrochemistry Communications 80, no. : 5-8.
In this work, we report the design, the development and the characterization of the analytical performances of a colorimetric smartphone-based immunosensor for the detection of cancer antigen 125 (CA125). The immunosensor was based on a sandwich strategy in which the primary antibody was immobilized by spotting onto the 3D nitrocellulose membrane. The immunospots were subsequently incubated with CA125 solutions, followed by the affinity reaction with a secondary antibody labeled with gold nanoparticles (AuNPs). The antibody-AuNPs captured onto immunospots induced the silver deposition from a silver enhancer solution leading to the formation of gold-silver nanoparticles of different grey color spots depending on CA125 concentration. The 8 megapixels smartphone camera was integrated in a home-made dark box and used as transducer of color image acquisition and data handling. The pixel intensity of the captured images was determined by an image processing algorithm. The experimental parameters involved in each step of the immunosensor design were studied and optimized, obtaining a limit of detection of 30U/mL CA125. The selectivity of the immunoassay was proven against different concentration solutions of Vascular Endothelial Growth Factor (VEGF) antigen as an unspecific protein when a blank signal was obtained for all tested solutions. Finally, preliminary experiments in human serum samples spiked with CA125 protein were also performed. Therefore, the proposed system could represent a powerful point-of-care tool for the next generation technology for detecting and monitoring cancer biomarkers at early stages by taking advantage of nowadays gadgets with enhanced features such as smartphones.
Oana Hosu; Andrea Ravalli; Giuseppe Mattia Lo Piccolo; Cecilia Cristea; Robert Sandulescu; Giovanna Marrazza. Smartphone-based immunosensor for CA125 detection. Talanta 2017, 166, 234 -240.
AMA StyleOana Hosu, Andrea Ravalli, Giuseppe Mattia Lo Piccolo, Cecilia Cristea, Robert Sandulescu, Giovanna Marrazza. Smartphone-based immunosensor for CA125 detection. Talanta. 2017; 166 ():234-240.
Chicago/Turabian StyleOana Hosu; Andrea Ravalli; Giuseppe Mattia Lo Piccolo; Cecilia Cristea; Robert Sandulescu; Giovanna Marrazza. 2017. "Smartphone-based immunosensor for CA125 detection." Talanta 166, no. : 234-240.