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Dr. Mihaela Tertiş
Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania

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Research Keywords & Expertise

0 Analytical Chemistry
0 Biosensors
0 Nanomaterials and nanostructures
0 molecularly imprinted polymer (MIP)
0 DNA aptamers

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Biosensors
molecularly imprinted polymer (MIP)

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Review
Published: 28 June 2021 in Sustainability
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Contamination of surface waters with pathogens as well as all diseases associated with such events are a significant concern worldwide. In recent decades, there has been a growing interest in developing analytical methods with good performance for the detection of this category of contaminants. The most important analytical methods applied for the determination of bacteria in waters are traditional ones (such as bacterial culturing methods, enzyme-linked immunoassay, polymerase chain reaction, and loop-mediated isothermal amplification) and advanced alternative methods (such as spectrometry, chromatography, capillary electrophoresis, surface-enhanced Raman scattering, and magnetic field-assisted and hyphenated techniques). In addition, optical and electrochemical sensors have gained much attention as essential alternatives for the conventional detection of bacteria. The large number of available methods have been materialized by many publications in this field aimed to ensure the control of water quality in water resources. This study represents a critical synthesis of the literature regarding the latest analytical methods covering comparative aspects of pathogen contamination of water resources. All these aspects are presented as representative examples, focusing on two important bacteria with essential implications on the health of the population, namely Pseudomonas aeruginosa and Escherichia coli.

ACS Style

Alexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability 2021, 13, 7229 .

AMA Style

Alexandra Canciu, Mihaela Tertis, Oana Hosu, Andreea Cernat, Cecilia Cristea, Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability. 2021; 13 (13):7229.

Chicago/Turabian Style

Alexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. 2021. "Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters." Sustainability 13, no. 13: 7229.

Review
Published: 27 May 2021 in Molecules
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Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.

ACS Style

Mihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules 2021, 26, 3200 .

AMA Style

Mihaela Tertis, Oana Hosu, Bogdan Feier, Andreea Cernat, Anca Florea, Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules. 2021; 26 (11):3200.

Chicago/Turabian Style

Mihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. 2021. "Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection." Molecules 26, no. 11: 3200.

Journal article
Published: 19 February 2021 in Journal of Personalized Medicine
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Methylene blue (MB) is both a dye and a medicine known and used for a long time including as lymphatic tracer in melanoma and breast cancer for revealing sentinel lymph nodes. Proflavine (PRO) is an acriflavine dye, used as bacteriostatic disinfectant against many gram-positive bacteria that was also successfully applied to evaluate morphopathological changes in tissues. This study was performed on a group of twenty-eight Wistar rats and had as its main objective the in vivo evaluation of the use of MB and PRO as perforator tracers. The two dyes proved to be effective functional perforasome tracers with medium inflammatory infiltrate in the skin of the island perforator flap which heals perfectly at 14 days with complete absence of the inflammatory reaction. At the same injected amount, PRO seems to determine a greater inflammatory reaction compared with MB, but in smaller concentration, the inflammatory response is absent in the case of PRO. In conclusion, both substances tested within this in vivo study are good functional perforasome tracers, but PRO has the advantage of the absence of inflammatory reaction when using lower concentrations, while preserving unalerted its efficiency as tracer.

ACS Style

Maria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study. Journal of Personalized Medicine 2021, 11, 147 .

AMA Style

Maria-Eliza Nedu, Mihaela Tertis, Cecilia Cristea, Alexandru Georgescu. Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study. Journal of Personalized Medicine. 2021; 11 (2):147.

Chicago/Turabian Style

Maria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. 2021. "Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study." Journal of Personalized Medicine 11, no. 2: 147.

Journal article
Published: 03 January 2021 in Biosensors
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The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5–65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2–65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1–100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5–100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer.

ACS Style

Iulia Rus; Mihaela Tertiș; Cristina Barbălată; Alina Porfire; Ioan Tomuță; Robert Săndulescu; Cecilia Cristea. An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer. Biosensors 2021, 11, 15 .

AMA Style

Iulia Rus, Mihaela Tertiș, Cristina Barbălată, Alina Porfire, Ioan Tomuță, Robert Săndulescu, Cecilia Cristea. An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer. Biosensors. 2021; 11 (1):15.

Chicago/Turabian Style

Iulia Rus; Mihaela Tertiș; Cristina Barbălată; Alina Porfire; Ioan Tomuță; Robert Săndulescu; Cecilia Cristea. 2021. "An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer." Biosensors 11, no. 1: 15.

Journal article
Published: 18 April 2020 in Sensors
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The detection of folic acid in biological samples or pharmaceutical products is of great importance due to its implications in the biological functions of the human body, along with the development and growth of the fetus. The deficiency of folic acid can be reversed by the intake of different pharmaceutical formulations or alimentary products fortified with this molecule. The elaboration of sensing platforms represents a continuous work in progress, a task in which the use of conductive polymers modified with different functionalities represents one of the outcoming strategies. The possibility of manipulating their morphology with the use of templates or surfactants represents another advantage. A sensing platform based on carboxylic functionalized polypyrrole was synthesized via the electrochemical approach in the presence of a polymeric surfactant on a graphite-based surface. The sensor was able to detect the folic acid from 2.5 μM to 200 μM with a calculated limited of detection of 0.8 μM. It was employed for the detection of the analyte from commercial human serum and pharmaceutical products with excellent recovery rates. The results were double checked using an optimized spectrophotometric procedure that confirmed furthermore the performances of the sensor related to real samples assessment.

ACS Style

Gheorghe Melinte; Andreea Cernat; Maria-Bianca Irimes; Szabolcs János Györfi; Mihaela Tertiș; Maria Suciu; Liana Anicăi; Robert Săndulescu; Cecilia Cristea. Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole. Sensors 2020, 20, 2315 .

AMA Style

Gheorghe Melinte, Andreea Cernat, Maria-Bianca Irimes, Szabolcs János Györfi, Mihaela Tertiș, Maria Suciu, Liana Anicăi, Robert Săndulescu, Cecilia Cristea. Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole. Sensors. 2020; 20 (8):2315.

Chicago/Turabian Style

Gheorghe Melinte; Andreea Cernat; Maria-Bianca Irimes; Szabolcs János Györfi; Mihaela Tertiș; Maria Suciu; Liana Anicăi; Robert Săndulescu; Cecilia Cristea. 2020. "Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole." Sensors 20, no. 8: 2315.

Review
Published: 15 April 2020 in Diagnostics
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Methylene blue and proflavine are fluorescent dyes used to stain nucleic acid from the molecular level to the tissue level. Already clinically used for sentinel node mapping, detection of neuroendocrine tumors, methemoglobinemia, septic shock, ifosfamide-induced encephalopathy, and photodynamic inactivation of RNA viruses, the antimicrobial, anti-inflammatory, and antioxidant effect of methylene blue has been demonstrated in different in vitro and in vivo studies. Proflavine was used as a disinfectant and bacteriostatic agent against many gram-positive bacteria, as well as a urinary antiseptic involved in highlighting cell nuclei. At the tissue level, the anti-inflammatory effects of methylene blue protect against pulmonary, renal, cardiac, pancreatic, ischemic-reperfusion lesions, and fevers. First used for their antiseptic and antiviral activity, respectively, methylene blue and proflavine turned out to be excellent dyes for diagnostic and treatment purposes. In vitro and in vivo studies demonstrated that both dyes are efficient as perfusion and tissue tracers and permitted to evaluate the minimal efficient concentration in different species, as well as their pharmacokinetics and toxicity. This review aims to identify the optimal concentrations of methylene blue and proflavine that can be used for in vivo experiments to highlight the vascularization of the skin in the case of a perforasome (both as a tissue tracer and in vascular mapping), as well as their effects on tissues. This review is intended to be a comparative and critical presentation of the possible applications of methylene blue (MB) and proflavine (PRO) in the surgical field, and the relevant biomedical findings from specialized literature to date are discussed as well.

ACS Style

Maria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications. Diagnostics 2020, 10, 223 .

AMA Style

Maria-Eliza Nedu, Mihaela Tertis, Cecilia Cristea, Alexandru Georgescu. Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications. Diagnostics. 2020; 10 (4):223.

Chicago/Turabian Style

Maria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. 2020. "Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications." Diagnostics 10, no. 4: 223.

Article
Published: 21 January 2020 in Journal of Applied Polymer Science
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The development of systems for targeted delivery of Sorafenib in unresectable hepatocellular carcinoma to reduce the systemic toxicity is a challenge. In our article, we successfully prepared core‐shell microcapsules based on bovine serum albumin gel with polyelectrolyte complex multilayer shell of polysaccharides with opposite charges, hyaluronic acid, and chitosan, encapsulating Sorafenib, as targeting delivery system for improved hepatocellular carcinoma therapy. A bovine serum albumin gel core was formed by a method based on a sacrificial CaCO3 template, followed by the multilayer shell build‐up of Ca2+ cross‐linked hyaluronic acid hydrogel, and subsequently alternating multilayers of the polyelectrolyte complex formed between hyaluronic acid and chitosan. The following techniques: Fourier‐transform infrared and UV–Vis spectroscopy, X‐ray diffraction, differential scanning calorimetry, confocal laser scanning microscopy, atomic force microscopy, and scanning electron microscopy were used for the physicochemical characterization. These tests revealed the spherical shape of core‐shell type, the micro‐size, as well as the composition of microcapsules after their synthesis and proved the successful encapsulation and release of the drug. The promising results regarding encapsulation efficiency, Sorafenib release profile and cytotoxicity on HepG2 and mesenchymal stem cells, recommend Sorafenib loaded microcapsules as suitable targeted drug carriers for further in vivo studies for hepatocellular carcinoma therapy.

ACS Style

Violeta Paşcalău; Mihaela Tertis; Emoke Pall; Maria Suciu; Traian Marinca; Marius Pustan; Violeta Merie; Iulia Alexandra Rus; Cristian Moldovan; Tamara Topala; Codruta Pavel; Catalin Popa. Bovine serum albumin gel/polyelectrolyte complex of hyaluronic acid and chitosan based microcarriers for Sorafenib targeted delivery. Journal of Applied Polymer Science 2020, 137, 1 .

AMA Style

Violeta Paşcalău, Mihaela Tertis, Emoke Pall, Maria Suciu, Traian Marinca, Marius Pustan, Violeta Merie, Iulia Alexandra Rus, Cristian Moldovan, Tamara Topala, Codruta Pavel, Catalin Popa. Bovine serum albumin gel/polyelectrolyte complex of hyaluronic acid and chitosan based microcarriers for Sorafenib targeted delivery. Journal of Applied Polymer Science. 2020; 137 (34):1.

Chicago/Turabian Style

Violeta Paşcalău; Mihaela Tertis; Emoke Pall; Maria Suciu; Traian Marinca; Marius Pustan; Violeta Merie; Iulia Alexandra Rus; Cristian Moldovan; Tamara Topala; Codruta Pavel; Catalin Popa. 2020. "Bovine serum albumin gel/polyelectrolyte complex of hyaluronic acid and chitosan based microcarriers for Sorafenib targeted delivery." Journal of Applied Polymer Science 137, no. 34: 1.

Review
Published: 01 October 2019 in Magnetochemistry
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During the last few decades, magnetic nanoparticles have been evaluated as promising materials in the field of cancer detection, screening, and treatment. Early diagnosis and screening of cancer may be achieved using magnetic nanoparticles either within the magnetic resonance imaging technique and/or sensing systems. These sensors are designed to selectively detect specific biomarkers, compounds that can be related to the onset or evolution of cancer, during and after the treatment of this widespread disease. Some of the particular properties of magnetic nanoparticles are extensively exploited in cancer therapy as drug delivery agents to selectively target the envisaged location by tailored in vivo manipulation using an external magnetic field. Furthermore, individualized treatment with antineoplastic drugs may be combined with magnetic resonance imaging to achieve an efficient therapy. This review summarizes the studies about the implications of magnetic nanoparticles in cancer diagnosis, treatment and drug delivery as well as prospects for future development and challenges of magnetic nanoparticles in the field of oncology.

ACS Style

Oana Hosu; Mihaela Tertis; Cecilia Cristea; Hosu. Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment. Magnetochemistry 2019, 5, 55 .

AMA Style

Oana Hosu, Mihaela Tertis, Cecilia Cristea, Hosu. Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment. Magnetochemistry. 2019; 5 (4):55.

Chicago/Turabian Style

Oana Hosu; Mihaela Tertis; Cecilia Cristea; Hosu. 2019. "Implication of Magnetic Nanoparticles in Cancer Detection, Screening and Treatment." Magnetochemistry 5, no. 4: 55.

Published erratum
Published: 10 June 2019 in Biosensors and Bioelectronics
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ACS Style

Mihaela Tertis; Petrica Ionut Leva; Diana Bogdan; Maria Suciu; Florin Graur; Cecilia Cristea. Corrigendum to “Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening” [Biosens. Bioelectron. 137 (2019) 123–132]. Biosensors and Bioelectronics 2019, 141, 111410 .

AMA Style

Mihaela Tertis, Petrica Ionut Leva, Diana Bogdan, Maria Suciu, Florin Graur, Cecilia Cristea. Corrigendum to “Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening” [Biosens. Bioelectron. 137 (2019) 123–132]. Biosensors and Bioelectronics. 2019; 141 ():111410.

Chicago/Turabian Style

Mihaela Tertis; Petrica Ionut Leva; Diana Bogdan; Maria Suciu; Florin Graur; Cecilia Cristea. 2019. "Corrigendum to “Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening” [Biosens. Bioelectron. 137 (2019) 123–132]." Biosensors and Bioelectronics 141, no. : 111410.

Research paper
Published: 06 June 2019 in Analytical and Bioanalytical Chemistry
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The rapid detection of bacterial strains has become a major topic thoroughly discussed across the biomedical field. Paired with the existence of nosocomial pathogen agents that imply extreme medical and financial challenges throughout diagnosis and treatment, the development of rapid and easy-to-use sensing devices has gained an increased amount of attention. Moreover, antibiotic resistance considered by World Health Organization as one of the “biggest threats to global health, food security, and development today” enables this topic as high priority. Pseudomonas aeruginosa, one of the most ubiquitous bacterial strains, has various quorum-sensing systems that are a direct cause of their virulence. One of them is represented by pyocyanin, a blue pigment with electroactive properties that is synthesized from early stages of bacterial colonization. Thus, the sensitive detection of this biomarker could enable a personalized and efficient therapy. It was achieved with the development of an electrochemical sensor based on a thermosensitive polymer, modified with Au/Ag nanoalloy for the rapid and accurate detection of pyocyanin, a virulence biomarker of Pseudomonas aeruginosa. The sensor displayed a linear range from 0.12 to 25 μM, and a limit of detection of 0.04 μM (signal/noise = 3). It was successfully tested in real samples spiked with the target analyte without any pretreatment other than a dilution step. The detection of pyocyanin with high recovery in whole blood in a time frame of 5–10 min from the moment of collection was performed with this electrochemical sensor.

ACS Style

Andreea Cernat; Alexandra Canciu; Mihaela Tertis; Florin Graur; Cecilia Cristea. Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin. Analytical and Bioanalytical Chemistry 2019, 411, 3829 -3838.

AMA Style

Andreea Cernat, Alexandra Canciu, Mihaela Tertis, Florin Graur, Cecilia Cristea. Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin. Analytical and Bioanalytical Chemistry. 2019; 411 (17):3829-3838.

Chicago/Turabian Style

Andreea Cernat; Alexandra Canciu; Mihaela Tertis; Florin Graur; Cecilia Cristea. 2019. "Synergic action of thermosensitive hydrogel and Au/Ag nanoalloy for sensitive and selective detection of pyocyanin." Analytical and Bioanalytical Chemistry 411, no. 17: 3829-3838.

Journal article
Published: 07 May 2019 in Biosensors and Bioelectronics
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Despite the fact that cancer research has experienced important advances and remarkable improvement in the curing processes during the last decades, this disease still occupies a leading position among the causes of death worldwide. It has been demonstrated that there is an interconnection between the overexpression of interleukin-6 cytokine and the tumor growth, metastasis, and therapeutic resistance in several types of malignancies. Herein, a highly sensitive and selective aptasensor for quantitative detection of interleukin-6 was developed by using a glassy carbon electrode modified with p-aminobenzoic acid, p-aminothiophenol and gold nanoparticles. A thio-terminated aptamer specific for interleukin-6 was immobilized on the surface of the modified electrode via the formation of gold-sulfur bonds. This DNA oligonucleotide was then used as a detection probe to capture the target protein at the biosensor surface allowing label-free detection by electrochemical impedance spectroscopy. The developed aptasensor showed a good linear response from 5 pgmL−1 to 100 ngmL−1 with a detection limit of 1.6 pgmL−1, within the range of physiological concentration of the protein. The biosensor exhibited high selectivity and has been successfully used to detect interleukin-6 in blood samples collected from patients suffering of colorectal cancer, with excellent recoveries after the addition of known amount of the target protein.

ACS Style

Mihaela Tertis; Petrica Ionut Leva; Diana Bogdan; Maria Suciu; Florin Graur; Cecilia Cristea. Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening. Biosensors and Bioelectronics 2019, 137, 123 -132.

AMA Style

Mihaela Tertis, Petrica Ionut Leva, Diana Bogdan, Maria Suciu, Florin Graur, Cecilia Cristea. Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening. Biosensors and Bioelectronics. 2019; 137 ():123-132.

Chicago/Turabian Style

Mihaela Tertis; Petrica Ionut Leva; Diana Bogdan; Maria Suciu; Florin Graur; Cecilia Cristea. 2019. "Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening." Biosensors and Bioelectronics 137, no. : 123-132.

Journal article
Published: 25 April 2019 in Electrochimica Acta
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A novel, simple and versatile nanocomposite platform based on graphene-oxide/chitosan modified screen printed electrode was optimized and used for the development of an immunosensor for the sensitive and highly selective electrochemical detection of serotonin. Graphene oxide has been used for providing carboxylic groups which form covalent bonds with the terminal amine groups from the antibody units, thus ensuring the immobilization of the biocomponent at the electrode surface. The presence of chitosan is necessary to ensure the stability of the nanomaterial film deposited on the electrode’ surface during the modification steps. The platform development protocol was optimized throughout the study by using electrochemical methods (electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry) as well as scanning electron microscopy. The following steps, namely immobilization of the antibody, blocking of the active free sites from graphene template with bovine serum albumin and testing of the immunosensor after immobilization of the analyte molecules at the surface, were optimized based on the differential pulse voltammetry tests following the signal obtained for the electrochemical oxidation of serotonin. The newly customized sensor was found to have a detection range from 10 nM to 100 μM and a limit of detection of 3.2 nM, being used for serotonin detection from human serum, saliva, artificial tears and urine samples as well as in the presence of different interferences, when good recovery rates were obtained.

ACS Style

Alina Adumitrăchioaie; Mihaela Tertiș; Maria Suciu; Florin Graur; Cecilia Cristea. A novel immunosensing platform for serotonin detection in complex real samples based on graphene oxide and chitosan. Electrochimica Acta 2019, 311, 50 -61.

AMA Style

Alina Adumitrăchioaie, Mihaela Tertiș, Maria Suciu, Florin Graur, Cecilia Cristea. A novel immunosensing platform for serotonin detection in complex real samples based on graphene oxide and chitosan. Electrochimica Acta. 2019; 311 ():50-61.

Chicago/Turabian Style

Alina Adumitrăchioaie; Mihaela Tertiș; Maria Suciu; Florin Graur; Cecilia Cristea. 2019. "A novel immunosensing platform for serotonin detection in complex real samples based on graphene oxide and chitosan." Electrochimica Acta 311, no. : 50-61.

Journal article
Published: 11 April 2019 in Materials
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Pyoverdine is a fluorescent siderophore produced by Pseudomonas aeruginosa that can be considered as a detectable marker in nosocomial infections. The presence of pyoverdine in water can be directly linked to the presence of the P. aeruginosa, thus being a nontoxic and low-cost marker for the detection of biological contamination. A novel platform was developed and applied for the electrochemical selective and sensitive detection of pyoverdine, based on a graphene/graphite-modified screen-printed electrode (SPE) that was electrochemically reduced and decorated with gold nanoparticles (NPs). The optimized sensor presenting higher sensitivity towards pyoverdine was successfully applied for its detection in real samples (serum, saliva, and tap water), in the presence of various interfering species. The excellent analytical performances underline the premises for an early diagnosis kit of bacterial infections based on electrochemical sensors.

ACS Style

Islem Gandouzi; Mihaela Tertis; Andreea Cernat; Dalila Saidane-Mosbahi; Aranka Ilea; Cecilia Cristea. A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors. Materials 2019, 12, 1180 .

AMA Style

Islem Gandouzi, Mihaela Tertis, Andreea Cernat, Dalila Saidane-Mosbahi, Aranka Ilea, Cecilia Cristea. A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors. Materials. 2019; 12 (7):1180.

Chicago/Turabian Style

Islem Gandouzi; Mihaela Tertis; Andreea Cernat; Dalila Saidane-Mosbahi; Aranka Ilea; Cecilia Cristea. 2019. "A Nanocomposite Based on Reduced Graphene and Gold Nanoparticles for Highly Sensitive Electrochemical Detection of Pseudomonas aeruginosa through Its Virulence Factors." Materials 12, no. 7: 1180.

Review article
Published: 23 February 2019 in TrAC Trends in Analytical Chemistry
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Saliva is a complex matrix that reflects hormonal, immunological, metabolic and nutritional state of a person. Moreover, it is considered the most appropriate biological fluid for scientific investigations regarding ethics. Saliva chemistry is an emerging field and mostly employed in toxicology and forensic medicine for analysing and detecting user drug addiction and alcohol abuse by mapping the blood and saliva data. The advantages of salivary testing include helping in the diagnosis, prognosis and surveillance of disease, alongside with the non-invasive and ease of collection properties. The need for fast, cost-effective and reliable methods for saliva investigation has led to high technological advances in developing sensing tools. Hence, increasing number of papers are reporting diseases signalling salivary biomarkers for local or systemic pathologies like oral cancer, endocrine and immunologic pathologies, infectious and neurological diseases etc. This review focuses on the recent advances and main challenges employed in the detection and quantification of salivary biomarkers by separation, immunological and electrochemical techniques and highlight the future perspectives of the point-of-care devices in diagnosing local or systemic pathologies.

ACS Style

Ioana Tiuca Gug; Mihaela Tertis; Oana Hosu; Cecilia Cristea. Salivary biomarkers detection: Analytical and immunological methods overview. TrAC Trends in Analytical Chemistry 2019, 113, 301 -316.

AMA Style

Ioana Tiuca Gug, Mihaela Tertis, Oana Hosu, Cecilia Cristea. Salivary biomarkers detection: Analytical and immunological methods overview. TrAC Trends in Analytical Chemistry. 2019; 113 ():301-316.

Chicago/Turabian Style

Ioana Tiuca Gug; Mihaela Tertis; Oana Hosu; Cecilia Cristea. 2019. "Salivary biomarkers detection: Analytical and immunological methods overview." TrAC Trends in Analytical Chemistry 113, no. : 301-316.

Review
Published: 14 February 2019 in Biosensors
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Background: Saliva has been recently proposed as an alternative to classic biofluid analyses due to both availability and reliability regarding the evaluation of various biomarkers. Biosensors have been designed for the assessment of a wide spectrum of compounds, aiding in the screening, diagnosis, and monitoring of pathologies and treatment efficiency. This literature review aims to present the development in the biosensors research and their utility using salivary assessment. Methods: a comprehensive literature search has been conducted in the PubMed database, using the keywords “saliva” and “sensor”. A two-step paper selection algorithm was devised and applied. Results: The 49 papers selected for the present review focused on assessing the salivary biomarkers used in general diseases, oral pathologies, and pharmacology. The biosensors proved to be reliable tools for measuring the salivary levels of biochemical metabolic compounds such as glucose, proteinases and proteins, heavy metals and various chemical compounds, microorganisms, oncology markers, drugs, and neurotransmitters. Conclusions: Saliva is a biofluid with a significant clinical applicability for the evaluation and monitoring of a patient’s general health. Biosensors designed for assessing a wide range of salivary biomarkers are emerging as promising diagnostic or screening tools for improving the patients’ quality of life.

ACS Style

Aranka Ilea; Vlad Andrei; Claudia Nicoleta Feurdean; Anida-Maria Băbțan; Nausica Bianca Petrescu; Radu Septimiu Câmpian; Adina Bianca Boșca; Bianca Ciui; Mihaela Tertiș; Robert Săndulescu; Cecilia Cristea. Saliva, a Magic Biofluid Available for Multilevel Assessment and a Mirror of General Health—A Systematic Review. Biosensors 2019, 9, 27 .

AMA Style

Aranka Ilea, Vlad Andrei, Claudia Nicoleta Feurdean, Anida-Maria Băbțan, Nausica Bianca Petrescu, Radu Septimiu Câmpian, Adina Bianca Boșca, Bianca Ciui, Mihaela Tertiș, Robert Săndulescu, Cecilia Cristea. Saliva, a Magic Biofluid Available for Multilevel Assessment and a Mirror of General Health—A Systematic Review. Biosensors. 2019; 9 (1):27.

Chicago/Turabian Style

Aranka Ilea; Vlad Andrei; Claudia Nicoleta Feurdean; Anida-Maria Băbțan; Nausica Bianca Petrescu; Radu Septimiu Câmpian; Adina Bianca Boșca; Bianca Ciui; Mihaela Tertiș; Robert Săndulescu; Cecilia Cristea. 2019. "Saliva, a Magic Biofluid Available for Multilevel Assessment and a Mirror of General Health—A Systematic Review." Biosensors 9, no. 1: 27.

Journal article
Published: 21 January 2019 in International Journal of Polymeric Materials and Polymeric Biomaterials
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ACS Style

Violeta Paşcalău; Emoke Pall; Mihaela Tertis; Maria Suciu; Cecilia Cristea; Gheorghe Borodi; Andreea Bodoki; Tamara Topală; Rares Stiufiuc; Alin Moldovan; Codruta Pavel; Traian Marinca; Catalin Popa. In vitro study of BSA gel/polyelectrolite complexes core shell microcapsules encapsulating doxorubicin for antitumoral targeted treatment. International Journal of Polymeric Materials and Polymeric Biomaterials 2019, 68, 60 -72.

AMA Style

Violeta Paşcalău, Emoke Pall, Mihaela Tertis, Maria Suciu, Cecilia Cristea, Gheorghe Borodi, Andreea Bodoki, Tamara Topală, Rares Stiufiuc, Alin Moldovan, Codruta Pavel, Traian Marinca, Catalin Popa. In vitro study of BSA gel/polyelectrolite complexes core shell microcapsules encapsulating doxorubicin for antitumoral targeted treatment. International Journal of Polymeric Materials and Polymeric Biomaterials. 2019; 68 (1-3):60-72.

Chicago/Turabian Style

Violeta Paşcalău; Emoke Pall; Mihaela Tertis; Maria Suciu; Cecilia Cristea; Gheorghe Borodi; Andreea Bodoki; Tamara Topală; Rares Stiufiuc; Alin Moldovan; Codruta Pavel; Traian Marinca; Catalin Popa. 2019. "In vitro study of BSA gel/polyelectrolite complexes core shell microcapsules encapsulating doxorubicin for antitumoral targeted treatment." International Journal of Polymeric Materials and Polymeric Biomaterials 68, no. 1-3: 60-72.

Research paper
Published: 09 January 2019 in Analytical and Bioanalytical Chemistry
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Surface plasmon resonance technique is highly sensitive to various processes taking place on a metal film and it has emerged as a powerful label-free method to study molecular binding processes taking place on a surface. Another important but less explored area of applications is the use of hybrid methods which combine electrochemistry with optical methods for better monitoring and understanding of biochemical processes. A detection method based on surface plasmon resonance was developed for ampicillin, applying electrochemical techniques for the elaboration and characterization of the aptasensing platform used in this study. Ampicillin is a broad-spectrum β-lactam antibiotic, used both in human and veterinary medicine for the treatment and prevention of primary respiratory, gastrointestinal, urogenital, and skin bacterial infections. It is widely used because of its broad spectrum and low cost. This widespread use can result in the presence of residues in the environment and in food leading to health problems for individuals who are hypersensitive to penicillins. The gold chip was functionalized through potential-assisted immobilization, using multipulse amperometry, first with a thiol-terminated aptamer, as a specific ligand and secondly, using the same procedure, with mercaptohexanol, used to cover the unoccupied binding sites on the gold surface in order to prevent the nonspecific adsorption of ampicillin molecules. After establishing the optimal conditions for the chip functionalization, different concentrations of ampicillin were detected in real time, in the range of 2.5–1000 μmol L−1, with a limit of detection of 1 μmol L−1, monitoring the surface plasmon resonance response. The selectivity of the aptasensor was proven in the presence of other antibiotics and drugs, and the method was successfully applied for the detection of ampicillin from river water.

ACS Style

Adrian Blidar; Bogdan Feier; Mihaela Tertis; Ramona Galatus; Cecilia Cristea. Electrochemical surface plasmon resonance (EC-SPR) aptasensor for ampicillin detection. Analytical and Bioanalytical Chemistry 2019, 411, 1053 -1065.

AMA Style

Adrian Blidar, Bogdan Feier, Mihaela Tertis, Ramona Galatus, Cecilia Cristea. Electrochemical surface plasmon resonance (EC-SPR) aptasensor for ampicillin detection. Analytical and Bioanalytical Chemistry. 2019; 411 (5):1053-1065.

Chicago/Turabian Style

Adrian Blidar; Bogdan Feier; Mihaela Tertis; Ramona Galatus; Cecilia Cristea. 2019. "Electrochemical surface plasmon resonance (EC-SPR) aptasensor for ampicillin detection." Analytical and Bioanalytical Chemistry 411, no. 5: 1053-1065.

Short communication
Published: 16 November 2018 in Electrochemistry Communications
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Chemical functionalization of graphene oxide represents a major challenge in chemical engineering with the aim of both improving the properties of the material while generating versatile platforms with a broad range of applications. The development of electrochemical (bio)sensors requires the controlled and rational immobilization of molecules as a key step in the enhancement of analytical performance. Click chemistry reactions represent an important strategy for the covalent linking of different compounds on a substrate via complementary azide or alkyne groups. The mild reaction conditions allow the preservation of the properties of biomolecules, while the orientation towards green chemistry enables a new range of biomedical applications. The azide group was inserted in the graphene oxide backbone by chemical functionalization and the resulting product was characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy and electrochemical impedance spectroscopy. The successful synthesis of the graphene-azide platform was also validated by electrochemical methods performed after clicking ethynylferrocene, an electroactive model molecule. The results show that this new approach is a versatile method for the covalent immobilization of biomolecules.

ACS Style

Andreea Cernat; Szabolcs János Györfi; Maria-Bianca Irimes; Mihaela Tertis; Andreea Bodoki; Ioana-Ecaterina Pralea; Maria Suciu; Cecilia Cristea. Click chemistry on azide-functionalized graphene oxide. Electrochemistry Communications 2018, 98, 23 -27.

AMA Style

Andreea Cernat, Szabolcs János Györfi, Maria-Bianca Irimes, Mihaela Tertis, Andreea Bodoki, Ioana-Ecaterina Pralea, Maria Suciu, Cecilia Cristea. Click chemistry on azide-functionalized graphene oxide. Electrochemistry Communications. 2018; 98 ():23-27.

Chicago/Turabian Style

Andreea Cernat; Szabolcs János Györfi; Maria-Bianca Irimes; Mihaela Tertis; Andreea Bodoki; Ioana-Ecaterina Pralea; Maria Suciu; Cecilia Cristea. 2018. "Click chemistry on azide-functionalized graphene oxide." Electrochemistry Communications 98, no. : 23-27.

Journal article
Published: 25 October 2018 in Sensors and Actuators B: Chemical
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Advanced glycated end markers are involved in oxidative stress by producing long-term damage to proteins in ageing processes, atherosclerosis, and diabetes. The present work describes a cavitas printed electrochemical sensor for the direct salivary detection of N€(Carboxymethyl)lysine, a major advanced glycated end compound. The highly flexible and bendable sensor is integrated on a customized mouthguard placed on a phantom jaw that imitated the structure of the human oral cavity. The disposable sensor is readily attached on the mouthguard before usage and detached after the electrochemical investigation. Thus, it can be replaced whenever required. The sensor exhibits high selectivity and sensitivity in the phosphate buffer with a limit of detection of 166 ng/ml (equivalent to 0.81 µM) (over a range of 0.5-2500 µg/ml (equivalent to 2.45 µM-12.24 mM) N€(Carboxymethyl)lysine by employing a differential pulse voltammetry analytical procedure. Moreover, the cavitas sensor was tested in raw, untreated human saliva, and promising recoveries were obtained. The cost advantages of the printing technique, short timescale of the measurements, long storage stability and ease of use are attractive properties of the new mouthguard sensor. This non-invasive oral sensor for salivary N€(Carboxymethyl)lysine monitoring could potentially provide useful real-time information regarding a wearer's health condition, and thus, it holds considerable promise for the improved management of chronic diseases.

ACS Style

Bianca Ciui; Mihaela Tertis; Claudia N. Feurdean; Aranka Ilea; Robert Sandulescu; Joseph Wang; Cecilia Cristea. Cavitas electrochemical sensor toward detection of N-epsilon (carboxymethyl)lysine in oral cavity. Sensors and Actuators B: Chemical 2018, 281, 399 -407.

AMA Style

Bianca Ciui, Mihaela Tertis, Claudia N. Feurdean, Aranka Ilea, Robert Sandulescu, Joseph Wang, Cecilia Cristea. Cavitas electrochemical sensor toward detection of N-epsilon (carboxymethyl)lysine in oral cavity. Sensors and Actuators B: Chemical. 2018; 281 ():399-407.

Chicago/Turabian Style

Bianca Ciui; Mihaela Tertis; Claudia N. Feurdean; Aranka Ilea; Robert Sandulescu; Joseph Wang; Cecilia Cristea. 2018. "Cavitas electrochemical sensor toward detection of N-epsilon (carboxymethyl)lysine in oral cavity." Sensors and Actuators B: Chemical 281, no. : 399-407.

Full paper
Published: 22 October 2018 in Electroanalysis
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A novel magnetoimmunosensor, designed for sensitive and selective quantification of interleukin 6, is herein reported. The experimental design involves the covalent immobilization of anti‐interleukin 6 antibody through an amidic bond formed with the carboxyl functionalities provided at the surface of protein G‐functionalized magnetic microparticles, assuring a sandwich‐type immunoassay with electrochemical label free detection. All the experimental parameters involved in the elaboration and testing protocol were optimized. A linear calibration plot between the charge transfer resistance and the logarithmic concentration of interleukin‐6 was achieved in the 1 pg mL−1 to 1 μg mL−1 range. A limit of quantification of 1 pg mL−1 and a detection limit of 0.3 pg mL−1 were obtained. The optimized magnetoimmunosensor showed an excellent selectivity against some potentially interfering proteins and has been successfully applied for the determination of target protein in human serum, proving its clinical relevance.

ACS Style

Mihaela Tertiş; Gheorghe Melinte; Bianca Ciui; Ioan Şimon; Rareş Ştiufiuc; Robert Săndulescu; Cecilia Cristea. A Novel Label Free Electrochemical Magnetoimmunosensor for Human Interleukin-6 Quantification in Serum. Electroanalysis 2018, 31, 282 -292.

AMA Style

Mihaela Tertiş, Gheorghe Melinte, Bianca Ciui, Ioan Şimon, Rareş Ştiufiuc, Robert Săndulescu, Cecilia Cristea. A Novel Label Free Electrochemical Magnetoimmunosensor for Human Interleukin-6 Quantification in Serum. Electroanalysis. 2018; 31 (2):282-292.

Chicago/Turabian Style

Mihaela Tertiş; Gheorghe Melinte; Bianca Ciui; Ioan Şimon; Rareş Ştiufiuc; Robert Săndulescu; Cecilia Cristea. 2018. "A Novel Label Free Electrochemical Magnetoimmunosensor for Human Interleukin-6 Quantification in Serum." Electroanalysis 31, no. 2: 282-292.