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Contamination of surface waters with pathogens as well as all diseases associated with such events are a significant concern worldwide. In recent decades, there has been a growing interest in developing analytical methods with good performance for the detection of this category of contaminants. The most important analytical methods applied for the determination of bacteria in waters are traditional ones (such as bacterial culturing methods, enzyme-linked immunoassay, polymerase chain reaction, and loop-mediated isothermal amplification) and advanced alternative methods (such as spectrometry, chromatography, capillary electrophoresis, surface-enhanced Raman scattering, and magnetic field-assisted and hyphenated techniques). In addition, optical and electrochemical sensors have gained much attention as essential alternatives for the conventional detection of bacteria. The large number of available methods have been materialized by many publications in this field aimed to ensure the control of water quality in water resources. This study represents a critical synthesis of the literature regarding the latest analytical methods covering comparative aspects of pathogen contamination of water resources. All these aspects are presented as representative examples, focusing on two important bacteria with essential implications on the health of the population, namely Pseudomonas aeruginosa and Escherichia coli.
Alexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability 2021, 13, 7229 .
AMA StyleAlexandra Canciu, Mihaela Tertis, Oana Hosu, Andreea Cernat, Cecilia Cristea, Florin Graur. Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters. Sustainability. 2021; 13 (13):7229.
Chicago/Turabian StyleAlexandra Canciu; Mihaela Tertis; Oana Hosu; Andreea Cernat; Cecilia Cristea; Florin Graur. 2021. "Modern Analytical Techniques for Detection of Bacteria in Surface and Wastewaters." Sustainability 13, no. 13: 7229.
Food safety and quality control pose serious issues to food industry and public health domains, in general, with direct effects on consumers. Any physical, chemical, or biological unexpected or unidentified food constituent may exhibit harmful effects on people and animals from mild to severe reactions. According to the World Health Organization (WHO), unsafe foodstuffs are especially dangerous for infants, young children, elderly, and chronic patients. It is imperative to continuously develop new technologies to detect foodborne pathogens and contaminants in order to aid the strengthening of healthcare and economic systems. In recent years, peptide-based sensors gained much attention in the field of food research as an alternative to immuno-, apta-, or DNA-based sensors. This review presents an overview of the electrochemical biosensors using peptides as molecular bio-recognition elements published mainly in the last decade, highlighting their possible application for rapid, non-destructive, and in situ analysis of food samples. Comparison with peptide-based optical and piezoelectrical sensors in terms of analytical performance is presented. Methods of foodstuffs pretreatment are also discussed.
Mihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules 2021, 26, 3200 .
AMA StyleMihaela Tertis, Oana Hosu, Bogdan Feier, Andreea Cernat, Anca Florea, Cecilia Cristea. Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection. Molecules. 2021; 26 (11):3200.
Chicago/Turabian StyleMihaela Tertis; Oana Hosu; Bogdan Feier; Andreea Cernat; Anca Florea; Cecilia Cristea. 2021. "Electrochemical Peptide-Based Sensors for Foodborne Pathogens Detection." Molecules 26, no. 11: 3200.
Illicit drugs use and abuse remains an increasing challenge for worldwide authorities and, therefore, it is important to have accurate methods to detect them in seized samples, biological fluids and wastewaters. They are recently classified as the latest group of emerging pollutants as their consumption increased tremendously in recent years. Nanomaterials have gained much attention over the last decade in the development of sensors for a myriad of applications. The applicability of these nanomaterials, functionalized or not, significantly increases and it is therefore highly suitable for use in the detection of illicit drugs. We have assessed the suitability of various nanoplatforms, such as graphene (GPH), multi-walled carbon nanotubes (MWCNTs), gold nanoparticles (AuNPs) and platinum nanoparticles (PtNPs) for the electrochemical detection of illicit drugs. GPH and MWCNTs were chosen as the most suitable platforms and cocaine, 3,4-methylendioxymethamfetamine (MDMA), 3-methylmethcathinone (MMC) and α-pyrrolidinovalerophenone (PVP) were tested. Due to the hydrophobicity of the nanomaterials-based platforms which led to low signals, two strategies were followed namely, pretreatment of the electrodes in sulfuric acid by cyclic voltammetry and addition of Tween 20 to the detection buffer. Both strategies led to an increase in the oxidation signal of illicit drugs. Binary mixtures of illicit drugs with common adulterants found in street samples were also investigated. The proposed strategies allowed the sensitive detection of illicit drugs in the presence of most adulterants. The suitability of the proposed sensors for the detection of illicit drugs in spiked wastewaters was finally assessed.
Ana-Maria Dragan; Florina Maria Truta; Mihaela Tertis; Anca Florea; Jonas Schram; Andreea Cernat; Bogdan Feier; Karolien De Wael; Cecilia Cristea; Radu Oprean. Electrochemical Fingerprints of Illicit Drugs on Graphene and Multi-Walled Carbon Nanotubes. Frontiers in Chemistry 2021, 9, 1 .
AMA StyleAna-Maria Dragan, Florina Maria Truta, Mihaela Tertis, Anca Florea, Jonas Schram, Andreea Cernat, Bogdan Feier, Karolien De Wael, Cecilia Cristea, Radu Oprean. Electrochemical Fingerprints of Illicit Drugs on Graphene and Multi-Walled Carbon Nanotubes. Frontiers in Chemistry. 2021; 9 ():1.
Chicago/Turabian StyleAna-Maria Dragan; Florina Maria Truta; Mihaela Tertis; Anca Florea; Jonas Schram; Andreea Cernat; Bogdan Feier; Karolien De Wael; Cecilia Cristea; Radu Oprean. 2021. "Electrochemical Fingerprints of Illicit Drugs on Graphene and Multi-Walled Carbon Nanotubes." Frontiers in Chemistry 9, no. : 1.
Background: Neurotransmitters are chemical messengers with crucial implication in the human body. Perturbations in the concentration of neurotransmitters can affect a multitude of mental and physical functions such as heart rate, sleep, appetite and mood. Thus, the sensitive detection of these compounds is a real need for a new generation of treatments. Methods: Simultaneous detection of two important neurotransmitters, namely dopamine and serotonin, was investigated in this study using differential pulse voltammetry. The optimization of several surface parameters was performed in order to choose the best electrode material for electrochemical oxidation of targets. Screen-printed electrodes based on carbon, gold and platinum and modified with different nanomaterials (carbon nanotubes, gold nanoparticles and carbon nanotubes decorated with gold nanoparticles) were tested. Results: Carbon-based electrodes modified with multiwall carbon nanotubes and gold nanoparticles were chosen after the optimization protocol. Linear correlations between the analytic signals obtained and the concentration of dopamine and serotonin, respectively were obtained with good sensitivity and the detection limits were 0.3 μM for dopamine and 0.8 μM for serotonin with no significant reciprocal influences. Selectivity studies were also performed, as well as tests in real samples (e.g., human serum, tears and saliva) complex matrices for which acceptable recoveries were obtained. Conclusion: The results obtained in this study can be considered as an important starting point for the development of a fast and simple method for selective and highly sensitive detection of neurotransmitters, with possible applications in the diagnosis of different pathologies and for monitoring the effectiveness of the applied drug treatment.
Florina Truţă; Mihaela Tertis; Cecilia Cristea; Florin Graur. Simultaneous Detection of Dopamine and Serotonin in Real Complex Matrices. Current Analytical Chemistry 2021, 17, 374 -384.
AMA StyleFlorina Truţă, Mihaela Tertis, Cecilia Cristea, Florin Graur. Simultaneous Detection of Dopamine and Serotonin in Real Complex Matrices. Current Analytical Chemistry. 2021; 17 (3):374-384.
Chicago/Turabian StyleFlorina Truţă; Mihaela Tertis; Cecilia Cristea; Florin Graur. 2021. "Simultaneous Detection of Dopamine and Serotonin in Real Complex Matrices." Current Analytical Chemistry 17, no. 3: 374-384.
Aptamers have come in the spotlight as bio-mimetic molecular recognition elements in the field of biomedicine due to various applications in diagnostics, drug delivery, therapeutics, and pharmaceutical analysis. Aptamers are composed of nucleic acid strands (DNA or RNA) that can specifically interact in a three-dimensional tailored design with the target molecule. The basic method to generate aptamers is Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Recent technological advances in aptamer selection allow for faster and cheaper production of a new generation of high-affinity aptamers compared to the traditional SELEX, which can last up to several months. Rigorous characterization performed by multiple research groups endorsed several well-defined aptamer sequences. Binding affinity, nature of the biomolecular interactions and structural characterization are of paramount importance for aptamer screening and development of applications. However, remarkable challenges still need to be dealt with before the aptamers can make great contributions to the biomedical field. Poor specificity and sensitivity, questionable clinical use, low drug loading, in vivo stability and toxicity are only some of the identified challenges. This review accounts for the 30th celebration of the SELEX technology underlining the most important aptamers’ achievements in the biomedical field within mostly the past five years. Aptamers’ advantages over antibodies are discussed. Because of potential clinical translational utility, insights of remarkable developments in aptamer-based methods for diagnosis and monitoring of disease biomarkers and pharmaceuticals are discussed focusing on the recent studies (2015–2020). The current challenges and promising opportunities for aptamers for therapeutic and theragnostic purposes are also presented.
Geanina Ștefan; Oana Hosu; Karolien De Wael; María Jesús Lobo-Castañón; Cecilia Cristea. Aptamers in biomedicine: Selection strategies and recent advances. Electrochimica Acta 2021, 376, 137994 .
AMA StyleGeanina Ștefan, Oana Hosu, Karolien De Wael, María Jesús Lobo-Castañón, Cecilia Cristea. Aptamers in biomedicine: Selection strategies and recent advances. Electrochimica Acta. 2021; 376 ():137994.
Chicago/Turabian StyleGeanina Ștefan; Oana Hosu; Karolien De Wael; María Jesús Lobo-Castañón; Cecilia Cristea. 2021. "Aptamers in biomedicine: Selection strategies and recent advances." Electrochimica Acta 376, no. : 137994.
Methylene blue (MB) is both a dye and a medicine known and used for a long time including as lymphatic tracer in melanoma and breast cancer for revealing sentinel lymph nodes. Proflavine (PRO) is an acriflavine dye, used as bacteriostatic disinfectant against many gram-positive bacteria that was also successfully applied to evaluate morphopathological changes in tissues. This study was performed on a group of twenty-eight Wistar rats and had as its main objective the in vivo evaluation of the use of MB and PRO as perforator tracers. The two dyes proved to be effective functional perforasome tracers with medium inflammatory infiltrate in the skin of the island perforator flap which heals perfectly at 14 days with complete absence of the inflammatory reaction. At the same injected amount, PRO seems to determine a greater inflammatory reaction compared with MB, but in smaller concentration, the inflammatory response is absent in the case of PRO. In conclusion, both substances tested within this in vivo study are good functional perforasome tracers, but PRO has the advantage of the absence of inflammatory reaction when using lower concentrations, while preserving unalerted its efficiency as tracer.
Maria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study. Journal of Personalized Medicine 2021, 11, 147 .
AMA StyleMaria-Eliza Nedu, Mihaela Tertis, Cecilia Cristea, Alexandru Georgescu. Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study. Journal of Personalized Medicine. 2021; 11 (2):147.
Chicago/Turabian StyleMaria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. 2021. "Methylene Blue and Proflavine as Intraarterial Marker for Functional Perforazome—Comparative Study." Journal of Personalized Medicine 11, no. 2: 147.
A novel hybrid composite of conductive poly(methylene blue) (PMB) and carbon nanotubes (CNT) was prepared for the detection of 5-aminosalicylic acid (5-ASA). Electrosynthesis of PMB with glassy carbon electrode (GCE) or with carbon nanotube modified GCE was done in ethaline deep eutectic solvent of choline chloride mixed with ethylene glycol and a 10% v/v aqueous solution. Different sensor architectures were evaluated in a broad range of pH values in a Britton-Robinson (BR) buffer using electrochemical techniques, chronoamperometry (CA), and differential pulse voltammetry (DPV), to determine the optimum sensor configuration for 5-ASA sensing. Under optimal conditions, the best analytical performance was obtained with CNT/PMBDES/GCE in 0.04 M BR buffer pH 7.0 in the range 5–100 µM 5-ASA using the DPV method, with an excellent sensitivity of 9.84 μA cm−2 μM−1 (4.9 % RSD, n = 5) and a detection limit (LOD) (3σ/slope) of 7.7 nM, outclassing most similar sensors found in the literature. The sensitivity of the same sensor obtained in CA (1.33 μA cm−2 μM−1) under optimal conditions (pH 7.0, E app = +0.40 V) was lower than that obtained by DPV. Simultaneous detection of 5-ASA and its analogue, acetaminophen (APAP), was successfully realized, showing a catalytic effect towards the electro-oxidation of both analytes, lowering their oxidation overpotential, and enhancing the oxidation peak currents and peak-to-peak separation as compared with the unmodified electrode. The proposed method is simple, sensitive, easy to apply, and economical for routine analysis.
Oana Hosu; Madalina Barsan; Robert Săndulescu; Cecilia Cristea; Christopher Brett. Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid. Sensors 2021, 21, 1161 .
AMA StyleOana Hosu, Madalina Barsan, Robert Săndulescu, Cecilia Cristea, Christopher Brett. Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid. Sensors. 2021; 21 (4):1161.
Chicago/Turabian StyleOana Hosu; Madalina Barsan; Robert Săndulescu; Cecilia Cristea; Christopher Brett. 2021. "Hybrid Nanocomposite Platform, Based on Carbon Nanotubes and Poly(Methylene Blue) Redox Polymer Synthesized in Ethaline Deep Eutectic Solvent for Electrochemical Determination of 5-Aminosalicylic Acid." Sensors 21, no. 4: 1161.
Lysozyme is an enzyme existing in multiple organisms where it plays various vital roles. The most important role is its antibacterial activity in the human body; in fact, it is also called “the body's own antibiotic”. Despite its proven utility, lysozyme can potentially trigger allergic reactions in sensitive individuals, even in trace amounts. Therefore, lysozyme determination in foods is becoming of paramount importance. Traditional detection methods are expensive, time-consuming and they cannot be applied for fast in-situ quantification. Electrochemical and optical sensors have attracted an increasing attention due to their versatility and ability to reduce the disadvantages of traditional methods. Using an aptamer as the bioreceptor, the sensor selectivity is amplified due to the specific recognition of the analyte. This review is presenting the progresses made in lysozyme determination by means of electrochemical and optical aptasensors in the last five years. A critical overview on the methodologies employed for aptamer immobilization and on the strategies for signal amplification of the assays will be described. Different optical and electrochemical aptasensors will be discussed and compared in terms of analytical performances, versatility and real samples applications.
Gheorghe Melinte; Giulia Selvolini; Cecilia Cristea; Giovanna Marrazza. Aptasensors for lysozyme detection: Recent advances. Talanta 2021, 226, 122169 .
AMA StyleGheorghe Melinte, Giulia Selvolini, Cecilia Cristea, Giovanna Marrazza. Aptasensors for lysozyme detection: Recent advances. Talanta. 2021; 226 ():122169.
Chicago/Turabian StyleGheorghe Melinte; Giulia Selvolini; Cecilia Cristea; Giovanna Marrazza. 2021. "Aptasensors for lysozyme detection: Recent advances." Talanta 226, no. : 122169.
The aim of this study was to develop a disposable, simple, fast, and sensitive sensor for the simultaneous electrochemical detection of doxorubicin (DOX) and simvastatin (SMV), which could be used in preclinical studies for the development of new pharmaceutical formulations for drug delivery. Firstly, the electrochemical behavior of each molecule was analyzed regarding the influence of electrode material, electrolyte solution, and scan rate. After this, the proper electrode material, electrolyte solution, and scan rate for both active substances were chosen, and a linear sweep voltammetry procedure was optimized for simultaneous detection. Two chronoamperometry procedures were tested, one for the detection of DOX in the presence of SMV, and the other one for the detection of DOX and SMV together. Finally, calibration curves for DOX and SMV in the presence of each other were obtained using both electrochemical methods and the results were compared. The use of amperometry allowed for a better limit of detection (DOX: 0.1 μg/mL; SMV: 0.7 μg/mL) than the one obtained in voltammetry (1.5 μg/mL for both drugs). The limits of quantification using amperometry were 0.5 μg/mL for DOX (dynamic range: 0.5–65 μg/mL) and 2 μg/mL for SMV (dynamic range: 2–65 μg/mL), while using voltammetry 1 μg/mL was obtained for DOX (dynamic range: 1–100 μg/mL) and 5 μg/mL for SMV (dynamic range: 5–100 μg/mL). This detection strategy represents a promising tool for the analysis of new pharmaceutical formulations for targeted drug delivery containing both drugs, whose association was proven to bring benefits in the treatment of cancer.
Iulia Rus; Mihaela Tertiș; Cristina Barbălată; Alina Porfire; Ioan Tomuță; Robert Săndulescu; Cecilia Cristea. An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer. Biosensors 2021, 11, 15 .
AMA StyleIulia Rus, Mihaela Tertiș, Cristina Barbălată, Alina Porfire, Ioan Tomuță, Robert Săndulescu, Cecilia Cristea. An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer. Biosensors. 2021; 11 (1):15.
Chicago/Turabian StyleIulia Rus; Mihaela Tertiș; Cristina Barbălată; Alina Porfire; Ioan Tomuță; Robert Săndulescu; Cecilia Cristea. 2021. "An Electrochemical Strategy for the Simultaneous Detection of Doxorubicin and Simvastatin for Their Potential Use in the Treatment of Cancer." Biosensors 11, no. 1: 15.
Nanomaterials with different morphologies have been important in the development of electrochemical sensors with applications in the biomedical field. In the last decade, there has been increasing interest in Bi-based nanostructures for sensing applications where green strategies are preferred. The template-free synthesis of Bi nanowires (BiNWs) involving deep eutectic solvents (DESs) as electrolytes has enabled the fabrication of a sensing platform with electrocatalytic activity towards hydrogen peroxide. The target molecule plays an important part in the development of cancers and its detection could shed light on the dynamics related to cellular metabolism, with applications in medical research. The BiNW-based platform is shown to detect the molecule of interest in the presence of common interfering agents and offers avenues for further development.
Andreea Cernat; Aurora Petica; Veronica Anastasoaie; Oana Lazar; Szabolcs János Györfi; Maria-Bianca Irimes; Geanina Stefan; Mihaela Tertis; Marius Enachescu; Liana Anicăi; Cecilia Cristea. Detection of hydrogen peroxide involving bismuth nanowires via template-free electrochemical synthesis using deep eutectic solvents. Electrochemistry Communications 2020, 121, 106869 .
AMA StyleAndreea Cernat, Aurora Petica, Veronica Anastasoaie, Oana Lazar, Szabolcs János Györfi, Maria-Bianca Irimes, Geanina Stefan, Mihaela Tertis, Marius Enachescu, Liana Anicăi, Cecilia Cristea. Detection of hydrogen peroxide involving bismuth nanowires via template-free electrochemical synthesis using deep eutectic solvents. Electrochemistry Communications. 2020; 121 ():106869.
Chicago/Turabian StyleAndreea Cernat; Aurora Petica; Veronica Anastasoaie; Oana Lazar; Szabolcs János Györfi; Maria-Bianca Irimes; Geanina Stefan; Mihaela Tertis; Marius Enachescu; Liana Anicăi; Cecilia Cristea. 2020. "Detection of hydrogen peroxide involving bismuth nanowires via template-free electrochemical synthesis using deep eutectic solvents." Electrochemistry Communications 121, no. : 106869.
We summarize herein the literature in the last decade, involving the use of nanomaterials and various (bio)recognition elements, such as antibodies, aptamers and molecularly imprinted polymers, for the development of sensitive and selective (bio)sensors for illicit drugs with a focus on electrochemical transduction systems. The use and abuse of illicit drugs remains an increasing challenge for worldwide authorities and, therefore, it is important to have accurate methods to detect them in seized samples, biological fluids and wastewaters. They are recently classified as the latest group of “emerging pollutants,” as their consumption has increased tremendously in recent years. Nanomaterials, antibodies, aptamers and molecularly imprinted polymers have gained much attention over the last decade in the development of (bio)sensors for a myriad of applications. The applicability of these (nano)materials, functionalized or not, has significantly increased, and are therefore highly suitable for use in the detection of drugs. Lately, such functionalized nanoscale materials have assisted in the detection of illicit drugs fingerprints, providing large surface area, functional groups and unique properties that facilitate sensitive and selective sensing. The review discusses the types of commonly abused drugs and their toxicological implications, classification of functionalized nanomaterials (graphene, carbon nanotubes), their fabrication, and their application on real samples in different fields of forensic science. Biosensors for drugs of abuse from the last decade's literature are then exemplified. It also offers insights into the prospects and challenges of bringing the functionalized nanobased technology to the end user in the laboratories or in-field.
Florina Truta; Anca Florea; Andreea Cernat; Mihaela Tertis; Oana Hosu; Karolien De Wael; Cecilia Cristea. Tackling the Problem of Sensing Commonly Abused Drugs Through Nanomaterials and (Bio)Recognition Approaches. Frontiers in Chemistry 2020, 8, 1 .
AMA StyleFlorina Truta, Anca Florea, Andreea Cernat, Mihaela Tertis, Oana Hosu, Karolien De Wael, Cecilia Cristea. Tackling the Problem of Sensing Commonly Abused Drugs Through Nanomaterials and (Bio)Recognition Approaches. Frontiers in Chemistry. 2020; 8 ():1.
Chicago/Turabian StyleFlorina Truta; Anca Florea; Andreea Cernat; Mihaela Tertis; Oana Hosu; Karolien De Wael; Cecilia Cristea. 2020. "Tackling the Problem of Sensing Commonly Abused Drugs Through Nanomaterials and (Bio)Recognition Approaches." Frontiers in Chemistry 8, no. : 1.
Lactate is a metabolite biomarker of tissue oxygenation and it can be used in medicine to evaluate a pathology or in sport activities to evaluate physical performance. Lactate level assessment is also important for food industry. This acid is found in food and beverages and the concentration level can be correlated with the freshness, stability and quality of several products. In this work, we present a smartphone-based enzymatic biosensor utilizing the unique colorimetric properties of the poly(aniline-co-anthranilic acid) (p(ANI-co-AA)) composite film coupled with lactate oxidase–horseradish peroxidase (LOx–HRP) enzymes. The enzymes are immobilized on the composite polymer film by adsorption and they catalyze a reversible redox color change of the host polymer from green to blue in the presence of l-lactate as the substrate. A smartphone was applied as color detector, for image acquisition and data handling. The free-of-charge Color Grab® application for Android OS was used to enable an easy and clear display of the sensor’s response, indicating remarkable changes in the optical features. The results were confirmed by spectrophotometric measurements. The developed colorimetric enzymatic biosensors were studied and optimized in relation to different experimental parameters. Moreover, the colorimetric enzymatic biosensor was applied to real matrices analysis. It has been shown by these studies that the colorimetric biosensors are promising as quick and simple tests for handheld analysis in various fields.
Giulia Selvolini; Ana-Maria Drăgan; Gheorghe Melinte; Cecila Cristea; Giovanna Marrazza. A Smart Colorimetric Sensor for the Enzymatic Detection of L-Lactate in Screening Analysis. Proceedings 2020, 60, 35 .
AMA StyleGiulia Selvolini, Ana-Maria Drăgan, Gheorghe Melinte, Cecila Cristea, Giovanna Marrazza. A Smart Colorimetric Sensor for the Enzymatic Detection of L-Lactate in Screening Analysis. Proceedings. 2020; 60 (1):35.
Chicago/Turabian StyleGiulia Selvolini; Ana-Maria Drăgan; Gheorghe Melinte; Cecila Cristea; Giovanna Marrazza. 2020. "A Smart Colorimetric Sensor for the Enzymatic Detection of L-Lactate in Screening Analysis." Proceedings 60, no. 1: 35.
The use of a photocatalyst (photosensitizer) which produces singlet oxygen instead of enzymes for oxidizing analytes creates opportunities for designing cost-efficient and sensitive photoelectrochemical sensors. We report that perfluoroisopropyl-substituted zinc phthalocyanine (F64PcZn) interacts specifically with a complex phenolic compound, the antibiotic rifampicin (RIF), but not with hydroquinone or another complex phenolic compound, the antibiotic doxycycline. The specificity is imparted by the selective preconcentration of RIF in the photocatalytic layer, as revealed by electrochemical and optical measurements, complemented by molecular modeling that confirms the important role of a hydrophobic cavity formed by the iso-perfluoropropyl groups of the photocatalyst. The preconcentration effect favorably enhances the RIF photoelectrochemical detection limit as well as sensitivity to nanomolar (ppb) concentrations, LOD = 7 nM (6 ppb) and 2.8 A·M-1·cm-2, respectively. The selectivity to RIF, retained in the photosensitizer layer, is further enhanced by the selective removal of all unretained phenols via simple washing of the electrodes with pure buffer. The utility of the sensor for analyzing municipal wastewater was demonstrated. This first demonstration of enhanced selectivity and sensitivity due to intrinsic interactions of a molecular photocatalyst (photosensitizer) with an analyte, without use of a biorecognition element, may allow the design of related, robust, simple, and viable sensors.
Adrian Blidar; Stanislav Trashin; Erik N. Carrión; Sergiu M. Gorun; Cecilia Cristea; Karolien De Wael. Enhanced Photoelectrochemical Detection of an Analyte Triggered by Its Concentration by a Singlet Oxygen-Generating Fluoro Photosensitizer. ACS Sensors 2020, 1 .
AMA StyleAdrian Blidar, Stanislav Trashin, Erik N. Carrión, Sergiu M. Gorun, Cecilia Cristea, Karolien De Wael. Enhanced Photoelectrochemical Detection of an Analyte Triggered by Its Concentration by a Singlet Oxygen-Generating Fluoro Photosensitizer. ACS Sensors. 2020; ():1.
Chicago/Turabian StyleAdrian Blidar; Stanislav Trashin; Erik N. Carrión; Sergiu M. Gorun; Cecilia Cristea; Karolien De Wael. 2020. "Enhanced Photoelectrochemical Detection of an Analyte Triggered by Its Concentration by a Singlet Oxygen-Generating Fluoro Photosensitizer." ACS Sensors , no. : 1.
The development of biosensors and bioassays has focused recently on small, easy to use in a decentralized and portable manner and low-cost analytical devices for sensing and quantitative determination of biomarkers. Biosensors provide numerous advantages over other analytical techniques, including high selectivity and sensitivity, portability, miniaturization and possibility of on-site monitoring. Immunosensors were heavily used during the last decades, since they combine the specificity of the immune reaction between antibody and its specific antigen with the sensitivity provided by the detection platform. More recently, aptamers started to be used for the design of biosensors due to their improved characteristic compared to the antibody-based sensors. In this chapter an overview of the latest trends in electrochemical sensors design for diagnosis purposes of several diseases, including cancer, cardiovascular and neurological disorders, is presented.
Mihaela Tertis; Oana Hosu; Anca Florea; Cecilia Cristea. Biosensors for Clinical Samples: Consideration and Approaches. Immunodiagnostic Technologies from Laboratory to Point-Of-Care Testing 2020, 1 -32.
AMA StyleMihaela Tertis, Oana Hosu, Anca Florea, Cecilia Cristea. Biosensors for Clinical Samples: Consideration and Approaches. Immunodiagnostic Technologies from Laboratory to Point-Of-Care Testing. 2020; ():1-32.
Chicago/Turabian StyleMihaela Tertis; Oana Hosu; Anca Florea; Cecilia Cristea. 2020. "Biosensors for Clinical Samples: Consideration and Approaches." Immunodiagnostic Technologies from Laboratory to Point-Of-Care Testing , no. : 1-32.
The development of rapid and sensitive devices for the simultaneous detection of neurotransmitters has critical implications for the clinical field and for the management of several diseases. Parkinson’s, Alzheimer’s disease, autism, schizophrenia, depression and anxiety are major healthcare challenges for which early diagnostics and personalized therapy are of great concern. Carbon-based nanomaterials and especially graphene-based nanomaterials associated with different architectures have been extensively studied and continue to represent the first line of approach in the development of nanoplatforms for electrochemical sensors. The simultaneous detection of analytes represents a critical point that could be addressed by designing new materials with the capacity to resolve their electrochemical signals. The results can be presented as a matrix that offers a broader viewpoint toward the balance of the neurotransmitter levels that are correlated with clinical symptoms for personalized diagnosis. The goal is to describe and evaluate, in a critical manner, the elaboration of graphene-based sensors that can be included in clinical applications. A major check point discussed throughout the paper is represented by the interference between different neurotransmitters that appear due to their overlapping signals and the strategies to address them to achieve simultaneous detection of as many molecules as possible and to be similar to in vivo experiments.
Andreea Cernat; Geanina Ştefan; Mihaela Tertis; Cecilia Cristea; Ioan Simon. An overview of the detection of serotonin and dopamine with graphene-based sensors. Bioelectrochemistry 2020, 136, 107620 .
AMA StyleAndreea Cernat, Geanina Ştefan, Mihaela Tertis, Cecilia Cristea, Ioan Simon. An overview of the detection of serotonin and dopamine with graphene-based sensors. Bioelectrochemistry. 2020; 136 ():107620.
Chicago/Turabian StyleAndreea Cernat; Geanina Ştefan; Mihaela Tertis; Cecilia Cristea; Ioan Simon. 2020. "An overview of the detection of serotonin and dopamine with graphene-based sensors." Bioelectrochemistry 136, no. : 107620.
Despite the fact that the electrochemical biosensors based on glucose oxidase represent the golden standard for the management of diabetes, the elaboration of nonenzymatic sensors became extensively studied as an out-of-the-box concept that aims to simplify the existing approach. An important point of view is represented by the low price of the sensing device that has positive effects for both end-users and healthcare systems. The enzyme-free sensors based on low-cost materials such as transition metals have similar analytical properties to the commercial ones while eliminating the issues associated with the presence of the enzyme, such as the stability issues and limited shelf-life. The development of nanoporous nanomaterials for biomedical applications and electrocatalysis was referred to as an alternative to the conventional methods due to their enlarged area, electrical properties, ease of functionalization and not least to their low cost. Herein, we report the development of an electrochemical nonenzymatic sensor for glucose based on 3D copper nanostructures with Ni foams as promotor of the enhanced nanoporous morphology. The sensors were successfully tested in the presence of the designated target, even in the presence of common interference agents found in biological samples.
Gheorghe Melinte; Andreea Cernat; Aurora Petica; Oana Lazar; Marius Enachescu; Liana Anicai; Cecilia Cristea. Electrochemical Non-Enzymatic Detection of Glucose Based on 3D Electroformed Copper on Ni Foam Nanostructures. Materials 2020, 13, 1 .
AMA StyleGheorghe Melinte, Andreea Cernat, Aurora Petica, Oana Lazar, Marius Enachescu, Liana Anicai, Cecilia Cristea. Electrochemical Non-Enzymatic Detection of Glucose Based on 3D Electroformed Copper on Ni Foam Nanostructures. Materials. 2020; 13 (12):1.
Chicago/Turabian StyleGheorghe Melinte; Andreea Cernat; Aurora Petica; Oana Lazar; Marius Enachescu; Liana Anicai; Cecilia Cristea. 2020. "Electrochemical Non-Enzymatic Detection of Glucose Based on 3D Electroformed Copper on Ni Foam Nanostructures." Materials 13, no. 12: 1.
: Recently, different strategies such as personalized therapy, dose adjustment, therapy monitoring and targeted drug delivery (disease-specific localization), were adopted for improving efficacy ratio of medicines that are currently in use. From a therapeutical point of view, to reach the optimal concentrations in the organ or tissue to reach the optimal concentration from a therapeutic point of view, in the target organ or tissue, it is necessary to increase the administered dose, which is accompanied by an increase in the frequency and intensity of the side effects, many of them undesirable and distressing for the patient. Therefore, designing targeted delivery systems for a specific organ or tissue is the ideal solution, ensuring the necessary concentrations at the site of action and avoiding or at least significantly reducing the side effects. An overview of the modern analytical techniques currently used for the drug delivery systems characterization was intended in this review with a focus on those commonly used.
Iulia Rus; Mihaela Tertis; Cecilia Cristea; Robert Sandulescu. Modern Analytical Techniques for Drug Delivery Systems Characterization. Current Analytical Chemistry 2020, 16, 1 .
AMA StyleIulia Rus, Mihaela Tertis, Cecilia Cristea, Robert Sandulescu. Modern Analytical Techniques for Drug Delivery Systems Characterization. Current Analytical Chemistry. 2020; 16 ():1.
Chicago/Turabian StyleIulia Rus; Mihaela Tertis; Cecilia Cristea; Robert Sandulescu. 2020. "Modern Analytical Techniques for Drug Delivery Systems Characterization." Current Analytical Chemistry 16, no. : 1.
The development of new biosensors with applications in various domains represents a contemporary challenge that it is extensively studied. The elaboration of cell-based biosensors has been a major outbreak in the sensing field due to their high sensitivity and specificity doubled by high stability and catalytic activity of the enzymatic systems included in the immobilized living cells. The major drawback of the conventional enzymatic biosensors represented by the loss of the enzymatic activity was eliminated by the immobilization of whole-cells on sensing platforms while maintaining their stability. The selection of the whole-cell, either mammalian or microorganism, orientates the detection towards heterogeneous compounds such as heavy metals, pollutants, foodborne pathogens, and biomedical biomarkers. By keeping the enzymes in a cellular environment, the enzymatic turnover was facilitated and their catalytic activity loss was greatly reduced. A key point is represented by the selection of suitable platforms that can ensure the stability of the cell and allow the monitoring of the metabolic transfer with the extracellular environment. Depending on the end-application, the cells could be coated with a protective polymeric layer or they can be immobilized in different biocompatible polymers. Moreover, adding carbon-based and/or metallic nanoparticles, their stability and catalytic properties are highly improved. The future trends in sensing strategies involve the association between different nanomaterials, miniaturization and the development of out-of the box sensing devices with improved analytical performances. The present chapter discusses multiple approaches for the elaboration of nanohybrid platforms, along with their advantages and limitations and it also underlines the materials used for designing wearable sensing devices.
Andreea Cernat; Bianca Ciui; Luminita Fritea; Mihaela Tertis; Cecilia Cristea. New Materials for the Construction of Electrochemical Cell-Based Biosensors. Handbook of Cell Biosensors 2020, 1 -26.
AMA StyleAndreea Cernat, Bianca Ciui, Luminita Fritea, Mihaela Tertis, Cecilia Cristea. New Materials for the Construction of Electrochemical Cell-Based Biosensors. Handbook of Cell Biosensors. 2020; ():1-26.
Chicago/Turabian StyleAndreea Cernat; Bianca Ciui; Luminita Fritea; Mihaela Tertis; Cecilia Cristea. 2020. "New Materials for the Construction of Electrochemical Cell-Based Biosensors." Handbook of Cell Biosensors , no. : 1-26.
The detection of folic acid in biological samples or pharmaceutical products is of great importance due to its implications in the biological functions of the human body, along with the development and growth of the fetus. The deficiency of folic acid can be reversed by the intake of different pharmaceutical formulations or alimentary products fortified with this molecule. The elaboration of sensing platforms represents a continuous work in progress, a task in which the use of conductive polymers modified with different functionalities represents one of the outcoming strategies. The possibility of manipulating their morphology with the use of templates or surfactants represents another advantage. A sensing platform based on carboxylic functionalized polypyrrole was synthesized via the electrochemical approach in the presence of a polymeric surfactant on a graphite-based surface. The sensor was able to detect the folic acid from 2.5 μM to 200 μM with a calculated limited of detection of 0.8 μM. It was employed for the detection of the analyte from commercial human serum and pharmaceutical products with excellent recovery rates. The results were double checked using an optimized spectrophotometric procedure that confirmed furthermore the performances of the sensor related to real samples assessment.
Gheorghe Melinte; Andreea Cernat; Maria-Bianca Irimes; Szabolcs János Györfi; Mihaela Tertiș; Maria Suciu; Liana Anicăi; Robert Săndulescu; Cecilia Cristea. Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole. Sensors 2020, 20, 2315 .
AMA StyleGheorghe Melinte, Andreea Cernat, Maria-Bianca Irimes, Szabolcs János Györfi, Mihaela Tertiș, Maria Suciu, Liana Anicăi, Robert Săndulescu, Cecilia Cristea. Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole. Sensors. 2020; 20 (8):2315.
Chicago/Turabian StyleGheorghe Melinte; Andreea Cernat; Maria-Bianca Irimes; Szabolcs János Györfi; Mihaela Tertiș; Maria Suciu; Liana Anicăi; Robert Săndulescu; Cecilia Cristea. 2020. "Selective Detection of Folic Acid Using 3D Polymeric Structures of 3-Carboxylic Polypyrrole." Sensors 20, no. 8: 2315.
Methylene blue and proflavine are fluorescent dyes used to stain nucleic acid from the molecular level to the tissue level. Already clinically used for sentinel node mapping, detection of neuroendocrine tumors, methemoglobinemia, septic shock, ifosfamide-induced encephalopathy, and photodynamic inactivation of RNA viruses, the antimicrobial, anti-inflammatory, and antioxidant effect of methylene blue has been demonstrated in different in vitro and in vivo studies. Proflavine was used as a disinfectant and bacteriostatic agent against many gram-positive bacteria, as well as a urinary antiseptic involved in highlighting cell nuclei. At the tissue level, the anti-inflammatory effects of methylene blue protect against pulmonary, renal, cardiac, pancreatic, ischemic-reperfusion lesions, and fevers. First used for their antiseptic and antiviral activity, respectively, methylene blue and proflavine turned out to be excellent dyes for diagnostic and treatment purposes. In vitro and in vivo studies demonstrated that both dyes are efficient as perfusion and tissue tracers and permitted to evaluate the minimal efficient concentration in different species, as well as their pharmacokinetics and toxicity. This review aims to identify the optimal concentrations of methylene blue and proflavine that can be used for in vivo experiments to highlight the vascularization of the skin in the case of a perforasome (both as a tissue tracer and in vascular mapping), as well as their effects on tissues. This review is intended to be a comparative and critical presentation of the possible applications of methylene blue (MB) and proflavine (PRO) in the surgical field, and the relevant biomedical findings from specialized literature to date are discussed as well.
Maria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications. Diagnostics 2020, 10, 223 .
AMA StyleMaria-Eliza Nedu, Mihaela Tertis, Cecilia Cristea, Alexandru Georgescu. Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications. Diagnostics. 2020; 10 (4):223.
Chicago/Turabian StyleMaria-Eliza Nedu; Mihaela Tertis; Cecilia Cristea; Alexandru Georgescu. 2020. "Comparative Study Regarding the Properties of Methylene Blue and Proflavine and Their Optimal Concentrations for In Vitro and In Vivo Applications." Diagnostics 10, no. 4: 223.