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Mr. Ilias Tsochantaridis
Department of Molecular Biology & Genetics, Democritus University of Thrace

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0 Cancer
0 Cancer Stem Cells
0 Aldehyde dehydrogenases
0 ALDH3A1
0 ALDH1B1

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Journal article
Published: 23 February 2021 in Plants
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Propolis is a resinous substance produced by bees that exhibits antimicrobial, immunostimulatory and antioxidant activity. Its use is common in functional foods, cosmetics and traditional medicine despite the fact that it demonstrates low extraction yields and inconsistency in non-toxic solvents. In this work, a new encapsulation and delivery system consisting of liposomes and cyclodextrins incorporating propolis polyphenols has been developed and characterized. The antioxidant, antimutagenic and antiaging properties of the system under normal and UVB-induced oxidative stress conditions were investigated in cultured skin cells and/or reconstituted skin model. Furthermore, the transcript accumulation for an array of genes involved in many skin-related processes was studied. The system exhibits significant polyphenol encapsulation efficiency, physicochemical stability as well as controlled release rate in appropriate conditions. The delivery system can retain the anti-mutagenic, anti-oxidative and anti-ageing effects of propolis polyphenols to levels similar and comparable to those of propolis methanolic extracts, making the system ideal for applications where non-toxic solvents are required and controlled release of the polyphenol content is desired.

ACS Style

Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants 2021, 10, 420 .

AMA Style

Eleni Spanidi, Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Sophia Letsiou, Nektarios Aligiannis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Ioannis Mourtzinos, Aglaia Pappa, Konstantinos Gardikis. A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications. Plants. 2021; 10 (2):420.

Chicago/Turabian Style

Eleni Spanidi; Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Sophia Letsiou; Nektarios Aligiannis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Ioannis Mourtzinos; Aglaia Pappa; Konstantinos Gardikis. 2021. "A New Controlled Release System for Propolis Polyphenols and Its Biochemical Activity for Skin Applications." Plants 10, no. 2: 420.

Journal article
Published: 06 January 2021 in Biomedicines
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Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding carboxylic acids. ALDHs participate in a variety of cellular mechanisms, such as metabolism, cell proliferation and apoptosis, as well as differentiation and stemness. Over the last few years, ALDHs have emerged as cancer stem cell markers in a wide spectrum of solid tumors and hematological malignancies. In this study, the pathophysiological role of ALDH1B1 in human colorectal adenocarcinoma was investigated. Human colon cancer HT29 cells were stably transfected either with human green fluorescent protein (GFP)-tagged ALDH1B1 or with an empty lentiviral expression vector. The overexpression of ALDH1B1 was correlated with altered cell morphology, decreased proliferation rate and reduced clonogenic efficiency. Additionally, ALDH1B1 triggered a G2/M arrest at 24 h post-cell synchronization, probably through p53 and p21 upregulation. Furthermore, ALDH1B1-overexpressing HT29 cells exhibited enhanced resistance against doxorubicin, fluorouracil (5-FU) and etoposide. Finally, ALDH1B1 induced increased migratory potential and displayed epithelial–mesenchymal transition (EMT) through the upregulation of ZEB1 and vimentin and the consequent downregulation of E-cadherin. Taken together, ALDH1B1 confers alterations in the cell morphology, cell cycle progression and gene expression, accompanied by significant changes in the chemosensitivity and migratory potential of HT29 cells, underlying its potential significance in cancer progression.

ACS Style

Ilias Tsochantaridis; Angelos Roupas; Georgia-Persephoni Voulgaridou; Alexandra Giatromanolaki; Michael I. Koukourakis; Mihalis I. Panayiotidis; Aglaia Pappa. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells. Biomedicines 2021, 9, 44 .

AMA Style

Ilias Tsochantaridis, Angelos Roupas, Georgia-Persephoni Voulgaridou, Alexandra Giatromanolaki, Michael I. Koukourakis, Mihalis I. Panayiotidis, Aglaia Pappa. Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells. Biomedicines. 2021; 9 (1):44.

Chicago/Turabian Style

Ilias Tsochantaridis; Angelos Roupas; Georgia-Persephoni Voulgaridou; Alexandra Giatromanolaki; Michael I. Koukourakis; Mihalis I. Panayiotidis; Aglaia Pappa. 2021. "Aldehyde Dehydrogenase 1B1 Is Associated with Altered Cell Morphology, Proliferation, Migration and Chemosensitivity in Human Colorectal Adenocarcinoma Cells." Biomedicines 9, no. 1: 44.

Journal article
Published: 01 July 2020 in Foods
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The aim of the present study was to investigate the antimicrobial potential of Sideritis raeseri subps. raeseri essential oil (EO) against common food spoilage and pathogenic microorganisms and evaluate its antioxidant and antiproliferative activity. The EO was isolated by steam distillation and analyzed by GC/MS. The main constituents identified were geranyl-p-cymene (25.08%), geranyl-γ-terpinene (15.17%), and geranyl-linalool (14.04%). Initially, its activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis, Salmonella Typhimurium, Pseudomonas fragi, Saccharomyces cerevisiae, and Aspergillus niger was screened by the disk diffusion method. Subsequently, minimum inhibitory concentration (MIC), non-inhibitory concentration (NIC), and minimum lethal concentration (MLC) values were determined. Growth inhibition of all microorganisms tested was documented, although it was significantly lower compared to gentamycin, ciproxin, and voriconazole, which were used as positive controls. In a next step, its direct antioxidant properties were examined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, and the IC50 values were determined. The potential cytoprotective activity of the oil against H2O2–induced oxidative stress and DNA damage was studied in human immortalized keratinocyte (HaCaT) cells using the comet assay. Finally, the antiproliferative activity of the oil was evaluated against a panel of cancer cell lines including A375, Caco2, PC3, and DU145 and the non-cancerous HaCaT cell line using the sulforhodamine B (SRB) assay, and the EC50 values were determined. The oil demonstrated weak radical scavenging activity, noteworthy cytoprotective activity against H2O2–induced oxidative stress and DNA damage in HaCaT cells, and antiproliferative activity against all cell lines tested, being more sensitive against the in vitro model of skin melanoma.

ACS Style

Gregoria Mitropoulou; Marianthi Sidira; Myria Skitsa; Ilias Tsochantaridis; Aglaia Pappa; Christos Dimtsoudis; Charalampos Proestos; Yiannis Kourkoutas. Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritisraeseri subps. raeseri Essential Oil. Foods 2020, 9, 860 .

AMA Style

Gregoria Mitropoulou, Marianthi Sidira, Myria Skitsa, Ilias Tsochantaridis, Aglaia Pappa, Christos Dimtsoudis, Charalampos Proestos, Yiannis Kourkoutas. Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritisraeseri subps. raeseri Essential Oil. Foods. 2020; 9 (7):860.

Chicago/Turabian Style

Gregoria Mitropoulou; Marianthi Sidira; Myria Skitsa; Ilias Tsochantaridis; Aglaia Pappa; Christos Dimtsoudis; Charalampos Proestos; Yiannis Kourkoutas. 2020. "Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritisraeseri subps. raeseri Essential Oil." Foods 9, no. 7: 860.

Journal article
Published: 30 June 2020 in Antioxidants
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In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. Finally, the extracts alleviated the UVB-induced up-regulation of MMPs-3, -7 and -9 in a model of reconstituted skin tissue. In conclusion, honey extracts exhibited significant photoprotective and antiaging properties under UVB exposure conditions and thus could be further exploited as promising agents for developing novel and naturally-based, antiaging cosmeceutical products.

ACS Style

Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants 2020, 9, 566 .

AMA Style

Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Dimitra Iliadi, Ilias Tsochantaridis, Panagiota Michail, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Sofia Karabournioti, Nektarios Aligiannis, Konstantinos Gardikis, Alex Galanis, Mihalis I. Panayiotidis, Aglaia Pappa. Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models. Antioxidants. 2020; 9 (7):566.

Chicago/Turabian Style

Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Dimitra Iliadi; Ilias Tsochantaridis; Panagiota Michail; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Sofia Karabournioti; Nektarios Aligiannis; Konstantinos Gardikis; Alex Galanis; Mihalis I. Panayiotidis; Aglaia Pappa. 2020. "Honey Extracts Exhibit Cytoprotective Properties against UVB-Induced Photodamage in Human Experimental Skin Models." Antioxidants 9, no. 7: 566.

Journal article
Published: 09 May 2019 in Antioxidants
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The aim of this study was to assess the antioxidant, photoprotective, and antiaging effects of Greek propolis. Propolis was subjected to n-heptane or methanol extraction. Total phenolic/flavonoid content and antioxidant potential were determined in the extracts. Promising extracts were evaluated for their cytoprotective properties using human immortalized keratinocyte (HaCaT) or reconstituted human skin tissue following exposure to UVB. Assessment of cytotoxicity, DNA damage, oxidative status, and gene/protein expression levels of various matrix metalloproteinases (MMPs) were performed. The propolis methanolic fractions exhibited higher total phenolic and flavonoid contents and significant in vitro antioxidant activity. Incubation of HaCaT cells with certain methanolic extracts significantly decreased the formation of DNA strand breaks following exposure to UVB and attenuated UVB-induced decrease in cell viability. The extracts had no remarkable effect on the total antioxidant status, but significantly lowered total protein carbonyl content used as a marker for protein oxidation in HaCaT cells. MMP-1, -3, -7, and -9, monitored as endpoints of antiaging efficacy, were significantly reduced by propolis following UVB exposure in a model of reconstituted skin tissue. In conclusion, propolis protects against the oxidative and photodamaging effects of UVB and could be further explored as a promising agent for developing natural antiaging strategies.

ACS Style

Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants 2019, 8, 125 .

AMA Style

Athanasios Karapetsas, Georgia-Persephoni Voulgaridou, Manolis Konialis, Ilias Tsochantaridis, Spyridon Kynigopoulos, Maria Lambropoulou, Maria-Ioanna Stavropoulou, Konstantina Stathopoulou, Nektarios Aligiannis, Petros Bozidis, Anna Goussia, Konstantinos Gardikis, Mihalis I. Panayiotidis, Aglaia Pappa. Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models. Antioxidants. 2019; 8 (5):125.

Chicago/Turabian Style

Athanasios Karapetsas; Georgia-Persephoni Voulgaridou; Manolis Konialis; Ilias Tsochantaridis; Spyridon Kynigopoulos; Maria Lambropoulou; Maria-Ioanna Stavropoulou; Konstantina Stathopoulou; Nektarios Aligiannis; Petros Bozidis; Anna Goussia; Konstantinos Gardikis; Mihalis I. Panayiotidis; Aglaia Pappa. 2019. "Propolis Extracts Inhibit UV-Induced Photodamage in Human Experimental In Vitro Skin Models." Antioxidants 8, no. 5: 125.