Blue light and ultra-violet (UV) light have been shown to influence plant growth, morphology, and quality. In this study, we investigated the effects of pre-harvest supplemental lighting using UV-A and blue (UV-A/Blue) light and red and blue (RB) light on growth and nutritional quality of lettuce grown hydroponically in two greenhouse experiments. The RB spectrum was applied pre-harvest for two days or nights, while the UV-A/Blue spectrum was applied pre-harvest for two or four days or nights. All pre-harvest supplemental lighting treatments had a same duration of 12 h with a photon flux density (PFD) of 171 μmol m⁻² s⁻¹. Results of both experiments showed that pre-harvest supplemental lighting using UV A/Blue or RB light can increase the growth and nutritional quality of lettuce grown hydroponically. The enhancement of lettuce growth and nutritional quality by the pre-harvest supplemental lighting was more effective under low daily light integral (DLI) compared to a high DLI and tended to be more effective when applied during the night, regardless of spectrum.

Winter–spring greenhouse vegetable production is limited by low-level natural light, resulting in decreased growth and quality. To investigate whether short-term pre-harvest supplemental lighting (SL) with light emitting diodes (LEDs) can address this issue, a study was conducted in a greenhouse in Dallas, Texas. Red leaf lettuce (Lactuca sativa L. ‘Red Mist’) plants grown in a hydroponic system were treated with daytime or nighttime SL with red (R) and blue (B) LEDs (RB-LED), blue and UVA LEDs (B/UVA-LED), or white LEDs (W-LED) for three days before harvest and compared to those without SL (control). All SL treatments provided a photon flux density of 167 μmol·m⁻²·s⁻¹ for 12 h daily. Compared with the control, SL treatments increased leaf thickness and greenness, antioxidant capacity, and concentrations of phytonutrients such as anthocyanins, carotenoids, and total phenolics; however, shoot fresh biomass and total leaf area were generally not affected by SL. There were no differences in all of the above traits among W-LED, RB-LED and B/UVA-LED. Compared with daytime SL, nighttime SL increased leaf greenness and carotenoid concentration. In summary, all three LEDs with different spectra were effective in improving lettuce quality as short-term pre-harvest SL sources and nighttime SL was more effective than daytime SL; however, plant fresh weight and total leaf area were not affected.

White light emitting diodes (LED) have commonly been used as a sole light source for the indoor production of microgreens. However, the response of microgreens to the inclusion of ultraviolet A (UVA) and/or far-red (FR) light to white LED light remains unknown. To investigate the effects of adding UVA and FR light to white LEDs on plant biomass, height, and the concentrations of phytochemicals, four species of microgreens including basil, cabbage, kale, and kohlrabi were grown under six light treatments. The first three treatments were white LED (control) and two UVA treatments (adding UVA to white LED for the whole growth period or for the last 5 days). Another three treatments consisted of adding FR to the first three treatments. The total photon flux density (TPFD) for all six light treatments was the same. The percentages of UVA and FR photons in the TPFD were 23% and 32%, respectively. Compared to white LEDs, adding UVA throughout the growth period did not affect plant height in all the species except for basil, where 9% reduction was observed regardless of the FR light. On the contrary, the addition of FR light increased plant heights by 9–18% for basil, cabbage, and kohlrabi, regardless of the UVA treatment, compared to white LED. Furthermore, regardless of UVA, adding FR to white LEDs reduced the plant biomass, total phenolic contents, and antioxidant concentrations for at least one species. There was no interaction between FR and UVA on all the above growth and quality traits for all the species. In summary, microgreens were more sensitive to the addition of FR light compared to UVA; however, the addition of FR to white LEDs may reduce yields and phytochemicals in some species.

Hydroponics has become a popular production technology for leafy greens in greenhouses. However, year-round production of cool-season leafy greens remains challenging due to costly heating and cooling during winter and summer seasons, depending on location. Therefore, the objective of this study is to investigate the effect of nutrient solution cooling and heating in deep-water hydroponic systems on the performance of several leafy green vegetables. Two experiments of nutrient solution cooling during the summer season and another two experiments of nutrient solution heating during the winter season were conducted in Texas, USA in 2020–2021. Lettuce (Lactuca sativa L.) ‘Bergams Green’ and ‘Red Mist’, Pak Choi (Brassica rapa subsp. chinensis) ‘Purple Magic’ and ‘White Stem’, and spinach (Spinacia oleracea L.) ‘Mandolin’ and ‘Seaside’ were grown in the summer experiments, and only the two lettuce cultivars were grown for the winter experiments. For both cooling and heating studies, six deep-water culture systems were used with two treatments: cooling (23 °C) vs. no cooling, and heating (22 °C) vs. no heating, with three replications in each experiment. In the nutrient solution cooling study, spinach was the most heat-sensitive species, and ‘Mandolin’ was more heat-tolerant than ‘Seaside,’ as evidenced by its lower mortality rate in both experiments. Lettuce and pak choi grew well and solution cooling increased shoot fresh weight in both lettuce cultivars and in ‘White Stem’ pak choi but not in ‘Purple Magic’ pak choi. Conversely, during the winter season, solution heating increased shoot fresh weight of both lettuce cultivars; however, ‘Red Mist’ was more responsive than ‘Bergams Green’ lettuce. These results indicate the potential to increase crop yield by controlling nutrient solution temperature throughout the year, depending on the season. Also, there were genotypic differences in both cooling and heating experiments, indicating that more research is needed to determine the species-dependent and even cultivar-dependent nutrient solution temperature control strategies to achieve optimum year-round production.

Salt tolerant ornamental plants can be irrigated with alternative water sources that are typically saline as a sustainable practice for urban landscaping, especially in arid and semi-arid regions. However, the salt tolerance of many ornamentals is not known. An eight-week greenhouse experiment was conducted to assess the relative salt tolerance of four perennial ornamentals, ‘Angelina’ (Sedum rupestre), ‘Autumn Joy’ (S. telephium), ‘Blue Spruce’ (S. reflexum), and ‘Blue Daze’ (Evolvulus glomeratus). The plants were grown in pots with potting mix substrate and irrigated with control or saline solutions. The electrical conductivities (EC) of the saline solutions were 5.0 and 10.0 mS/cm. Data collected included relative shoot, root, and total dry weight (DW), visual score, shoot tissue concentrations of Na⁺, Cl⁻, K⁺, and Ca²⁺, and the K⁺/Na⁺ ratio. There were significant differences in treatment and varieties for all response variables, and some interactions were also significant, indicating different responses to salinity for the four varieties. Shoot, root, and total DW decreased with increasing salinity for all varieties. Visual score was highest in Autumn Joy and Blue Spruce when treated with EC5 and EC10 and lowest in Angelina and Blue Daze, the latter of which showed symptoms of moderate foliar damage including leaf necrosis, or “burn”, due to salt stress. The concentrations of Na⁺ and Cl⁻ in the shoot tissue increased with increasing salinity while K⁺ and Ca²⁺ and the K⁺/Na⁺ ratio tended to decrease. Of the four varieties of herbaceous perennial ornamentals evaluated in this study, Autumn Joy and Blue Spruce were considered the most relatively salt tolerant while Angelina and Blue Daze were least tolerant.

A greenhouse study was conducted to assess the relative salt tolerance of 11 cultivars of hydrangea: Hydrangea macrophylla ‘Ayesha’, ‘Emotion’, ‘Mathilda Gutges’, ‘Merritt’s Supreme’ and ‘Passion’; H. paniculata ‘Interhydia’ and ‘Bulk’; H. quercifolia ‘Snowflake’; H. serrata ‘Preciosa’; and H. serrata × macrophylla ‘Sabrina’ and ‘Selina’. Plants were treated with a nutrient solution at an electrical conductivity (EC) of 1.0 dS·m⁻¹, and nutrient solution-based saline solutions at an EC of 5.0 dS·m⁻¹ (EC 5) or 10 dS·m⁻¹ (EC 10). The study was repeated in time (Experiments 1 and 2). In both experiments, by the fourth week after treatment, ‘Bulk’ plants in EC 10 exhibited severe salt damage with most of them dead. ‘Interhydia’ was also sensitive, showing severe salt damage in EC 10 with a high mortality rate by the end of the experiment. The leaf area and total shoot dry weight (DW) of all cultivars in EC 5 and EC 10 treatments were significantly reduced compared to the control. Leaf sodium (Na⁺) and chloride (Cl⁻) concentrations were negatively correlated with visual quality, leaf area and shoot DW. The salt-sensitive cultivars ‘Bulk’, ‘Interhydia’ and ‘Snowflake’ had inherently low leaf Na⁺ and Cl⁻ concentrations in both control and salt-treated plants compared to other cultivars. Salt tolerance varied among species and cultivars within H. macrophylla. Among the 11 cultivars, H. macrophylla ‘Ayesha’ and two hybrids, ‘Sabrina’ and ‘Selina’, were relatively salt-tolerant. H. macrophylla ‘Merritt’s Supreme’ and ‘Mathilda’ were moderately tolerant. H. paniculata ‘Bulk’ was the most sensitive, followed by H. paniculata ‘Interhydia’, and then by H. serrata ‘Preciosa’ and H. macrophylla ‘Passion’, as evidenced by high mortality and severe salt damage symptoms. H. quercifolia ‘Snowflake’ and H. macrophylla ‘Emotion’ were moderately salt-sensitive.