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Many applications of immunoassays involve the possible presence of structurally similar compounds that bind with antibodies, but with different affinities. In this regard, an important characteristic of an immunoassay is its cross-reactivity: the possibility of detecting various compounds in comparison with a certain standard. Based on cross-reactivity, analytical systems are assessed as either high-selective (responding strictly to a specific compound) or low-selective (responding to a number of similar compounds). The present study demonstrates that cross-reactivity is not an intrinsic characteristic of antibodies but can vary for different formats of competitive immunoassays using the same antibodies. Assays with sensitive detection of markers and, accordingly, implementation at low concentrations of antibodies and modified (competing) antigens are characterized by lower cross-reactivities and are, thus, more specific than assays requiring high concentrations of markers and interacting reagents. This effect was confirmed by both mathematical modeling and experimental comparison of an enzyme immunoassay and a fluorescence polarization immunoassay of sulfonamides and fluoroquinolones. Thus, shifting to lower concentrations of reagents decreases cross-reactivities by up to five-fold. Moreover, the cross-reactivities are changed even in the same assay format by varying the ratio of immunoreactants’ concentrations and shifting from the kinetic or equilibrium mode of the antigen-antibody reaction. The described patterns demonstrate the possibility of modulating immunodetection selectivity without searching for new binding reactants.
Dmitriy Sotnikov; Anatoly Zherdev; Elena Zvereva; Sergei Eremin; Boris Dzantiev. Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation. Applied Sciences 2021, 11, 6581 .
AMA StyleDmitriy Sotnikov, Anatoly Zherdev, Elena Zvereva, Sergei Eremin, Boris Dzantiev. Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation. Applied Sciences. 2021; 11 (14):6581.
Chicago/Turabian StyleDmitriy Sotnikov; Anatoly Zherdev; Elena Zvereva; Sergei Eremin; Boris Dzantiev. 2021. "Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation." Applied Sciences 11, no. 14: 6581.
The concentration of glycocholic acid (GCA) in urine and blood is an important biomarker for liver cancer.
Qiyi He; Yanxiong Fang; Huiyi Yang; Ding Shen; Yingshan Chen; Yingying Zhong; Xiang-Guang Li; Sergei A. Eremin; Xiping Cui; Suqing Zhao. Enhanced performance of a surface plasmon resonance-based immunosensor for the detection of glycocholic acid. Analytical Methods 2021, 13, 1919 -1924.
AMA StyleQiyi He, Yanxiong Fang, Huiyi Yang, Ding Shen, Yingshan Chen, Yingying Zhong, Xiang-Guang Li, Sergei A. Eremin, Xiping Cui, Suqing Zhao. Enhanced performance of a surface plasmon resonance-based immunosensor for the detection of glycocholic acid. Analytical Methods. 2021; 13 (16):1919-1924.
Chicago/Turabian StyleQiyi He; Yanxiong Fang; Huiyi Yang; Ding Shen; Yingshan Chen; Yingying Zhong; Xiang-Guang Li; Sergei A. Eremin; Xiping Cui; Suqing Zhao. 2021. "Enhanced performance of a surface plasmon resonance-based immunosensor for the detection of glycocholic acid." Analytical Methods 13, no. 16: 1919-1924.
A hapten-protein conjugate with copper nanoparticles ([email protected]) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive determination of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of the GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the [email protected] conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8–32.3 μg·kg−1, with a limit of detection 0.08 μg·kg−1. The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks, and soil samples.
Elena V. Dorozhko; Anna S. Gashevskay; Elena I. Korotkova; Jiri Barek; Vlastimil Vyskocil; Sergei A. Eremin; Evgeny V. Galunin; Muhammad Saqib. A copper nanoparticle-based electrochemical immunosensor for carbaryl detection. Talanta 2021, 228, 122174 .
AMA StyleElena V. Dorozhko, Anna S. Gashevskay, Elena I. Korotkova, Jiri Barek, Vlastimil Vyskocil, Sergei A. Eremin, Evgeny V. Galunin, Muhammad Saqib. A copper nanoparticle-based electrochemical immunosensor for carbaryl detection. Talanta. 2021; 228 ():122174.
Chicago/Turabian StyleElena V. Dorozhko; Anna S. Gashevskay; Elena I. Korotkova; Jiri Barek; Vlastimil Vyskocil; Sergei A. Eremin; Evgeny V. Galunin; Muhammad Saqib. 2021. "A copper nanoparticle-based electrochemical immunosensor for carbaryl detection." Talanta 228, no. : 122174.
Industrialization is serious for changing the environment and natural water composition, especially near cities and manufacturing areas. Logically, the new ultrasensitive technology for precise control of the quality and quantity of water sources is needed. Herein, an innovative method of polarization fluorescence analysis (FPA) was developed to measure the concentration of heavy metals in water. The approach was successfully applied for precise tests with reduced analysis time and increased measurement efficiency among laboratory methods. Based on this work, the investigations established the new type of carbon quantum dots (CQDs) with controllable fluorescence properties and functionalized amino—groups, which is appropriate for FPA. The parameters of one and two-step microwave synthesis routes are adjusted wavelength and fluorescence intensity of CQDs. Finally, under optimized conditions, the FPA is showed the detection of copper (2+) cations in water samples below European Union standard (2 mg/L). Moreover, in comparison with fluorescence quenching, polarization fluorescence is proved as a convenient, simple, and rapid test method for effective water safety analysis.
Anastasia Yakusheva; Dmitry S. Muratov; Dmitry Arkhipov; Gopalu Karunakaran; Sergei A. Eremin; Denis Kuznetsov. Water-Soluble Carbon Quantum Dots Modified by Amino Groups for Polarization Fluorescence Detection of Copper (II) Ion in Aqueous Media. Processes 2020, 8, 1573 .
AMA StyleAnastasia Yakusheva, Dmitry S. Muratov, Dmitry Arkhipov, Gopalu Karunakaran, Sergei A. Eremin, Denis Kuznetsov. Water-Soluble Carbon Quantum Dots Modified by Amino Groups for Polarization Fluorescence Detection of Copper (II) Ion in Aqueous Media. Processes. 2020; 8 (12):1573.
Chicago/Turabian StyleAnastasia Yakusheva; Dmitry S. Muratov; Dmitry Arkhipov; Gopalu Karunakaran; Sergei A. Eremin; Denis Kuznetsov. 2020. "Water-Soluble Carbon Quantum Dots Modified by Amino Groups for Polarization Fluorescence Detection of Copper (II) Ion in Aqueous Media." Processes 8, no. 12: 1573.
Florfenicol amine, florfenicol, and thiamphenicol are equally recognized in the developed ELISA and can be quantified using a universal standard curve. These amphenicols approved for livestock use can be distinguished from banned chloramphenicol.
Inna A. Galvidis; Sergei A. Eremin; Maksim A. Burkin. Three for the price of one! Immunodetection of three amphenicols in foodstuffs using a universal standard curve. Analytical Methods 2020, 12, 1728 -1735.
AMA StyleInna A. Galvidis, Sergei A. Eremin, Maksim A. Burkin. Three for the price of one! Immunodetection of three amphenicols in foodstuffs using a universal standard curve. Analytical Methods. 2020; 12 (13):1728-1735.
Chicago/Turabian StyleInna A. Galvidis; Sergei A. Eremin; Maksim A. Burkin. 2020. "Three for the price of one! Immunodetection of three amphenicols in foodstuffs using a universal standard curve." Analytical Methods 12, no. 13: 1728-1735.
Antibodies against the common 2-desoxystreptamine fragment of aminoglycosides are capable of revealing the wide spectrum of analytes in honey at a 10 μg kg−1level.
Inna A. Galvidis; Konstantin M. Burkin; Sergei A. Eremin; Maksim A. Burkin. Group-specific detection of 2-deoxystreptamine aminoglycosides in honey based on antibodies against ribostamycin. Analytical Methods 2019, 11, 4620 -4628.
AMA StyleInna A. Galvidis, Konstantin M. Burkin, Sergei A. Eremin, Maksim A. Burkin. Group-specific detection of 2-deoxystreptamine aminoglycosides in honey based on antibodies against ribostamycin. Analytical Methods. 2019; 11 (36):4620-4628.
Chicago/Turabian StyleInna A. Galvidis; Konstantin M. Burkin; Sergei A. Eremin; Maksim A. Burkin. 2019. "Group-specific detection of 2-deoxystreptamine aminoglycosides in honey based on antibodies against ribostamycin." Analytical Methods 11, no. 36: 4620-4628.
Wheat grains collected in three regions of Russia—Volga, Ural, and West Siberia—were analyzed for triangulation of methods in analysis of toxigenic Fusarium species. The presence of fungi and quantitative content of their biomass were detected by using various analytical methods, including a mycological and immunochemical methods, and quantitative PCR. Additionally, an enzyme-linked immunosorbent assay and high-performance liquid chromatography with tandem mass spectrometry were applied for determination of mycotoxins. Regional differences were found regarding the contamination of wheat grain by Fusarium fungi and their toxins. The most important observation was the detection of F. graminearum in the Ural and West Siberian regions, where this pathogen had not been found previously. A maximum damaged grains by F. graminearum and F. sporotrichioides was found in the grain samples from West Siberia. The DNA of F. graminearum was detected in 19.2% and DNA of F. sporotrichioides was found in 84.1% of the analyzed grain samples. The amount of Fusarium antigens in the grain samples from the West Siberian region was 7–8 times higher than in the grain samples from the other two regions. Significant contamination of the grain with deoxynivalenol and T-2/HT-2 toxins (maximum contents were 2239 ppb and 199 ppb, respectively) was detected in the West Siberian region.
Tatiana Gagkaeva; Olga Gavrilova; Aleksandra Orina; Yuri Lebedin; Ilya Shanin; Pavel Petukhov; Sergei Eremin. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins 2019, 11, 252 .
AMA StyleTatiana Gagkaeva, Olga Gavrilova, Aleksandra Orina, Yuri Lebedin, Ilya Shanin, Pavel Petukhov, Sergei Eremin. Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia. Toxins. 2019; 11 (5):252.
Chicago/Turabian StyleTatiana Gagkaeva; Olga Gavrilova; Aleksandra Orina; Yuri Lebedin; Ilya Shanin; Pavel Petukhov; Sergei Eremin. 2019. "Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia." Toxins 11, no. 5: 252.
Conjugation chemistry does not always provide adequate spatial orientation of hapten in immunogens for the best presentation of generic or individual epitopes. In the present study, the influence of unique and multiple orientations of immunizing hapten on the immune response repertoire was compared to select generic recognition system. The glycopeptides, teicoplanin (TPL) and ristomycin (RSM), were conjugated to BSA to produce immunogens with unique and multiple orientations of haptens. Polyclonal antibodies generated against TPL conjugated through a single site were of uniform specificity and demonstrated selective TPL recognition, regardless of the coating conjugates design. The sensitivity (IC50) of 4 enzyme-linked immunosorbent assays (ELISAs) for TPL varied little within the 3.5–7.4 ng/mL, with a dynamic range of 0.2–100 ng/mL. RSM was coupled to BSA through several glycoside sites that evoked a wider repertoire of response. This first described anti-RSM antibody was selective for RSM in homologous hapten-coated ELISAs with IC50 values in the range 4.2–35 ng/mL. Among the heterologous antigens, periodate-oxidized TPL conjugated to gelatine was selected as the best binder of generic anti-RSM fraction. The developed ELISA showed group recognition of glycopeptides RSM, TPL, eremomycin, and vancomycin with cross-reactivity of 37–100% and a 10–10,000 ng/mL dynamic range. Thus, multiple presentations of immunizing hapten help expand the repertoire of immune responses and opportunities for the selection of the required fine-specificity agent.
Maksim A. Burkin; Inna A. Galvidis; Sergei A. Eremin. Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten. Biosensors 2019, 9, 52 .
AMA StyleMaksim A. Burkin, Inna A. Galvidis, Sergei A. Eremin. Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten. Biosensors. 2019; 9 (2):52.
Chicago/Turabian StyleMaksim A. Burkin; Inna A. Galvidis; Sergei A. Eremin. 2019. "Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten." Biosensors 9, no. 2: 52.
Fluorescence polarization (FP) assays play an important role in the detection of chemical contaminants in food and environmental due to a number of key advantages since last century. In recent decades, FP assays have achieved great progress thanks to developments in materials science and labelling techniques. This comprehensive review provides the state of art in methods design, recognition elements, fluorophores, multi-targeted analysis, practical applications with real samples, and the challenges of deploying FP assays for the detection of chemical contaminants. These new information and insightful commentary of the review will be critically important to the development and innovation of next-generation FP assays.
Huiyan Zhang; Shupeng Yang; Karl De Ruyck; Natalia V. Beloglazova; Sergei A. Eremin; Sarah De Saeger; Suxia Zhang; Jianzhong Shen; Zhanhui Wang. Fluorescence polarization assays for chemical contaminants in food and environmental analyses. TrAC Trends in Analytical Chemistry 2019, 114, 293 -313.
AMA StyleHuiyan Zhang, Shupeng Yang, Karl De Ruyck, Natalia V. Beloglazova, Sergei A. Eremin, Sarah De Saeger, Suxia Zhang, Jianzhong Shen, Zhanhui Wang. Fluorescence polarization assays for chemical contaminants in food and environmental analyses. TrAC Trends in Analytical Chemistry. 2019; 114 ():293-313.
Chicago/Turabian StyleHuiyan Zhang; Shupeng Yang; Karl De Ruyck; Natalia V. Beloglazova; Sergei A. Eremin; Sarah De Saeger; Suxia Zhang; Jianzhong Shen; Zhanhui Wang. 2019. "Fluorescence polarization assays for chemical contaminants in food and environmental analyses." TrAC Trends in Analytical Chemistry 114, no. : 293-313.
Detection of antibiotics in the blood is necessary for characterizing their common or individual pharmacokinetics. This has increased the need in rapid detection techniques, such as lateral flow immunoassay, for the on-site control of antibiotics. The present study characterized factors influencing the analytical parameters of lateral flow immunoassay to increase its sensitivity for detecting tetracycline in human serum samples. Assay sensitivity was increased by altering the concentrations of immunoreagents and surfactant and the number of interaction stages in the assay with indirect labeling a specific antibody. The optimal assay conditions reduced the limit of visual detection of tetracycline from 100 to 10 ng/mL. The developed assay allowed us to detect tetracycline in both two-fold diluted and undiluted human serum samples within 15 min. Our results suggest that the developed assay can be used to screen patients under antibiotic treatment.
Anna N. Berlina; Anastasia V. Bartosh; Anatoly V. Zherdev; Sergei A. Eremin; Boris B. Dzantiev. Management of Factors for Improving Antigen–Antibody Interaction in Lateral flow Immunoassay of Tetracycline in Human Serum Samples. Biomedical and Pharmacology Journal 2019, 12, 17 -24.
AMA StyleAnna N. Berlina, Anastasia V. Bartosh, Anatoly V. Zherdev, Sergei A. Eremin, Boris B. Dzantiev. Management of Factors for Improving Antigen–Antibody Interaction in Lateral flow Immunoassay of Tetracycline in Human Serum Samples. Biomedical and Pharmacology Journal. 2019; 12 (1):17-24.
Chicago/Turabian StyleAnna N. Berlina; Anastasia V. Bartosh; Anatoly V. Zherdev; Sergei A. Eremin; Boris B. Dzantiev. 2019. "Management of Factors for Improving Antigen–Antibody Interaction in Lateral flow Immunoassay of Tetracycline in Human Serum Samples." Biomedical and Pharmacology Journal 12, no. 1: 17-24.
A common problem in the immunodetection of structurally close compounds is understanding the regularities of immune recognition, and elucidating the basic structural elements that provide it. Correct identification of these elements would allow for select immunogens to obtain antibodies with either wide specificity to different representatives of a given chemical class (for class-specific immunoassays), or narrow specificity to a unique compound (mono-specific immunoassays). Fluoroquinolones (FQs; antibiotic contaminants of animal-derived foods) are of particular interest for such research. We studied the structural basis of immune recognition of FQs by antibodies against ciprofloxacin (CIP) and clinafloxacin (CLI) as the immunizing hapten. CIP and CLI possess the same cyclopropyl substituents at the N1 position, while their substituents at C7 and C8 are different. Anti-CIP antibodies were specific to 22 of 24 FQs, while anti-CLI antibodies were specific to 11 of 26 FQs. The molecular size was critical for the binding between the FQs and the anti-CIP antibody. The presence of the cyclopropyl ring at the N1 position was important for the recognition between fluoroquinolones and the anti-CLI antibody. The anti-CIP quantitative structure–activity relationship (QSAR) model was well-equipped to predict the test set (pred_R2 = 0.944). The statistical parameters of the anti-CLI model were also high (R2 = 0.885, q2 = 0.864). Thus, the obtained QSAR models yielded sufficient correlation coefficients, internal stability, and predictive ability. This work broadens our knowledge of the molecular mechanisms of FQs’ interaction with antibodies, and it will contribute to the further development of antibiotic immunoassays.
Andrey A. Buglak; Ilya A. Shanin; Sergei A. Eremin; Hong-Tao Lei; Xiangmei Li; Anatoly V. Zherdev; Boris B. Dzantiev. Ciprofloxacin and Clinafloxacin Antibodies for an Immunoassay of Quinolones: Quantitative Structure–Activity Analysis of Cross-Reactivities. International Journal of Molecular Sciences 2019, 20, 265 .
AMA StyleAndrey A. Buglak, Ilya A. Shanin, Sergei A. Eremin, Hong-Tao Lei, Xiangmei Li, Anatoly V. Zherdev, Boris B. Dzantiev. Ciprofloxacin and Clinafloxacin Antibodies for an Immunoassay of Quinolones: Quantitative Structure–Activity Analysis of Cross-Reactivities. International Journal of Molecular Sciences. 2019; 20 (2):265.
Chicago/Turabian StyleAndrey A. Buglak; Ilya A. Shanin; Sergei A. Eremin; Hong-Tao Lei; Xiangmei Li; Anatoly V. Zherdev; Boris B. Dzantiev. 2019. "Ciprofloxacin and Clinafloxacin Antibodies for an Immunoassay of Quinolones: Quantitative Structure–Activity Analysis of Cross-Reactivities." International Journal of Molecular Sciences 20, no. 2: 265.
Three approaches improving the sensitivity of previously established immunoassays for broad detection of sulfonamides (SAs) in milk are described.
Maksim A. Burkin; Gennady B. Lapa; Inna A. Galvidis; Konstantin M. Burkin; Alexander V. Zubkov; Sergei A. Eremin. Three steps improving the sensitivity of sulfonamide immunodetection in milk. Analytical Methods 2018, 10, 5773 -5782.
AMA StyleMaksim A. Burkin, Gennady B. Lapa, Inna A. Galvidis, Konstantin M. Burkin, Alexander V. Zubkov, Sergei A. Eremin. Three steps improving the sensitivity of sulfonamide immunodetection in milk. Analytical Methods. 2018; 10 (48):5773-5782.
Chicago/Turabian StyleMaksim A. Burkin; Gennady B. Lapa; Inna A. Galvidis; Konstantin M. Burkin; Alexander V. Zubkov; Sergei A. Eremin. 2018. "Three steps improving the sensitivity of sulfonamide immunodetection in milk." Analytical Methods 10, no. 48: 5773-5782.
In this paper, a simple, rapid and high-throughput fluorescence polarization immunoassay (FPIA) based on polyclonal antibodies (PAb) is described for the determination of glycocholic acid (GCA) in human urine. Three fluorescein-labeled GCA (tracers) with different structures and spacer bridges were synthesized and purified by thin-layer chromatography (TLC). The structure effect of tracers on the assay was investigated and the sensitivity of best tracer in the optimized FPIA demonstrated an IC50 value of 306 ng/mL. The working range of FPIA was 36 ∼ 2 600 ng/mL and the limit of detection (LOD) was 9 ng/mL. The developed FPIA was time-saving that could be completed within 10 min. Human urine samples spiked with GCA were analyzed by this method, followed by confirmation with commercial enzyme immunoassay analysis (EIA). Excellent recoveries and correlation between these two methods were observed (R2 = 0.996), suggesting the developed FPIA could be applied to screening of GCA in human urine samples without complicated cleanup.
Qiyi He; Xiping Cui; Ding Shen; Yingshan Chen; Zhengyun Jiang; Rui Lv; Sergei A. Eremin; Suqing Zhao. Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urine. Journal of Pharmaceutical and Biomedical Analysis 2018, 158, 431 -437.
AMA StyleQiyi He, Xiping Cui, Ding Shen, Yingshan Chen, Zhengyun Jiang, Rui Lv, Sergei A. Eremin, Suqing Zhao. Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urine. Journal of Pharmaceutical and Biomedical Analysis. 2018; 158 ():431-437.
Chicago/Turabian StyleQiyi He; Xiping Cui; Ding Shen; Yingshan Chen; Zhengyun Jiang; Rui Lv; Sergei A. Eremin; Suqing Zhao. 2018. "Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urine." Journal of Pharmaceutical and Biomedical Analysis 158, no. : 431-437.
Fluorescence polarization immunoassays (FPIAs) for thiabendazole and tetraconazole were first developed. Tracers for FPIAs of thiabendazole and tetraconazole were synthesized and the tracers’ structures were confirmed by HPLC-MS/MS. The 4-aminomethylfluorescein-labeled tracers allowed achieving the best assay sensitivity and minimum reagent consumption in comparison with aminofluorescein-labeled and alkyldiaminefluoresceinthiocarbamyl-labeled tracers. Measurements of fluorescence polarization were performed using a portable device. The developed FPIA methods were applied for the analysis of wheat. Fast and simple sample preparation technique earlier developed by authors for pesticides was adapted for thiabendazole and tetraconazole. The limits of detection of thiabendazole and tetraconazole in wheat were 20 and 200 μg/kg, and the lower limits of quantification were 40 and 600 μg/kg, respectively. The recovery test was performed by two methods—FPIA and HPLC-MS/MS. The results obtained by FPIA correlated well with those obtained by HPLC-MS/MS (r2 = 0.9985 for thiabendazole, r2 = 0.9952 for tetraconazole). Average recoveries of thiabendazole and tetraconazole were 74 ± 4% and 72 ± 3% by FPIA, and average recoveries of thiabendazole and tetraconazole were 86 ± 2% and 74 ± 1% by HPLC-MS/MS (n = 15).
Anna Yu. Boroduleva; Juan J. Manclús; Ángel Montoya; Sergei A. Eremin. Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat. Analytical and Bioanalytical Chemistry 2018, 410, 6923 -6934.
AMA StyleAnna Yu. Boroduleva, Juan J. Manclús, Ángel Montoya, Sergei A. Eremin. Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat. Analytical and Bioanalytical Chemistry. 2018; 410 (26):6923-6934.
Chicago/Turabian StyleAnna Yu. Boroduleva; Juan J. Manclús; Ángel Montoya; Sergei A. Eremin. 2018. "Fluorescence polarization immunoassay for rapid screening of the pesticides thiabendazole and tetraconazole in wheat." Analytical and Bioanalytical Chemistry 410, no. 26: 6923-6934.
In this study, a fluorescence polarization immunoassay (FPIA) technique was developed to determine colchicine (COL), an alkaloid of noxious plants of the order Liliales that is used in a number of medications to treat gout. An optimal combination of the polyclonal antibody and the antigen labelled with fluorescein isothiocyanate (FITC) was selected. Conditions for the competitive interaction of the antigen in the tested samples and its fluorophore conjugate (COL–FITC) with anti-COL antibodies were optimised, and the analytical characteristics of the assay were determined. The developed FPIA was characterised by a detection limit of 1.8 ng/mL and a detectable analyte concentration range of 4.1–74.3 ng/mL. The duration of the analysis was 10 min. The applicability of the developed FPIA for quality control of ready-made drug formulations and for the estimation of COL content in various matrices (urine, milk), with recovery values ranging from 79–108%, was demonstrated.
Elena A. Zvereva; Anatoly Zherdev; Andey A. Formanovsky; Ramadan A. Abuknesha; Sergei A. Eremin; Boris B. Dzantiev. Fluorescence polarization immunoassay of colchicine. Journal of Pharmaceutical and Biomedical Analysis 2018, 159, 326 -330.
AMA StyleElena A. Zvereva, Anatoly Zherdev, Andey A. Formanovsky, Ramadan A. Abuknesha, Sergei A. Eremin, Boris B. Dzantiev. Fluorescence polarization immunoassay of colchicine. Journal of Pharmaceutical and Biomedical Analysis. 2018; 159 ():326-330.
Chicago/Turabian StyleElena A. Zvereva; Anatoly Zherdev; Andey A. Formanovsky; Ramadan A. Abuknesha; Sergei A. Eremin; Boris B. Dzantiev. 2018. "Fluorescence polarization immunoassay of colchicine." Journal of Pharmaceutical and Biomedical Analysis 159, no. : 326-330.
Dipstick device developed on the principle of lateral flow using gold nanoparticles for analysis of morphine in urine by morphine/scFv/immunoprobe.
Sonu Gandhi; Ivneet Banga; Pawan Kumar Maurya; Sergei A. Eremin. A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick. RSC Advances 2018, 8, 1511 -1518.
AMA StyleSonu Gandhi, Ivneet Banga, Pawan Kumar Maurya, Sergei A. Eremin. A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick. RSC Advances. 2018; 8 (3):1511-1518.
Chicago/Turabian StyleSonu Gandhi; Ivneet Banga; Pawan Kumar Maurya; Sergei A. Eremin. 2018. "A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick." RSC Advances 8, no. 3: 1511-1518.
Fluorescence polarization immunoassays (FPIA) were developed for the determination of pesticides triazophos and carbaryl in wheat grains using the portable FPIA device Sentry 200 (Ellie).
Anna Yu Boroduleva; Jing Wu; Qingqing Yang; Hui Li; Qi Zhang; Peiwu Li; Sergei A. Eremin. Development of fluorescence polarization immunoassays for parallel detection of pesticides carbaryl and triazophos in wheat grains. Analytical Methods 2017, 9, 6814 -6822.
AMA StyleAnna Yu Boroduleva, Jing Wu, Qingqing Yang, Hui Li, Qi Zhang, Peiwu Li, Sergei A. Eremin. Development of fluorescence polarization immunoassays for parallel detection of pesticides carbaryl and triazophos in wheat grains. Analytical Methods. 2017; 9 (48):6814-6822.
Chicago/Turabian StyleAnna Yu Boroduleva; Jing Wu; Qingqing Yang; Hui Li; Qi Zhang; Peiwu Li; Sergei A. Eremin. 2017. "Development of fluorescence polarization immunoassays for parallel detection of pesticides carbaryl and triazophos in wheat grains." Analytical Methods 9, no. 48: 6814-6822.
A homogeneous fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of imidaclothiz was developed. Two fluorescein-labeled imidaclothiz tracers containing two different bridge lengths were synthesized and purified. Under optimal conditions, the 4-aminofluorescein-labeled imidaclothiz conjugate (AMF-labeled imidaclothiz), which contains a shorter bridge length, showed a higher sensitivity in the FPIA for detecting imidaclothiz, and the full analysis was achieved in less than 11 min. The IC and limit of detection (LOD, IC) were 87.94 ± 10.18 and 0.57 ± 0.16 μg/L, respectively. The spiked recoveries were 83 to 117 % measured in tomato, pear, rice, apple, cucumber, cabbage, and paddy water, with RSDs of 5 to 12 %. Furthermore, the results of FPIA for the authentic samples correlated well with those acquired by HPLC. Overall, the developed FPIA provided a simple, rapid, sensitive, and accurate method that was used for the quantitative detection of imidaclothiz in agricultural samples.
Ming Ma; Mo Chen; Lu Feng; Hongjie You; Rui Yang; Anna Boroduleva; Xiude Hua; Sergei A. Eremin; Minghua Wang. Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples. Food Analytical Methods 2016, 9, 2471 -2478.
AMA StyleMing Ma, Mo Chen, Lu Feng, Hongjie You, Rui Yang, Anna Boroduleva, Xiude Hua, Sergei A. Eremin, Minghua Wang. Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples. Food Analytical Methods. 2016; 9 (9):2471-2478.
Chicago/Turabian StyleMing Ma; Mo Chen; Lu Feng; Hongjie You; Rui Yang; Anna Boroduleva; Xiude Hua; Sergei A. Eremin; Minghua Wang. 2016. "Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samples." Food Analytical Methods 9, no. 9: 2471-2478.