Robert Pokluda

Prof. Robert Pokluda

Mendel University in Brno, Brno, Jihomoravský Kraj, Czech Republic
orcid

Research Focus & Metrics

Academic Career Length

26Years (2001-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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31 March 2017 in Horticultural Science
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Journal Article
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Agnieszka SękaraAgnieszka Sękara
Agnieszka Sękara
RPRobert Pokluda
Robert Pokluda
LVLaura Del Vacchio
Laura Del Vacchio
SSSilvano Somma
Silvano Somma
GCGianluca Caruso
Gianluca Caruso
» Articles | Agricultural Journals

8 March 2018 in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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TKTomáš Kopta
Tomáš Kopta
MPMarcela Pavlíková
Marcela Pavlíková
Agnieszka SękaraAgnieszka Sękara
Agnieszka Sękara
RPRobert Pokluda
Robert Pokluda
BMBlahoslav Maršálek
Blahoslav Maršálek
Plant biostimulants can enhance crop nutrition status, stress tolerance, yield and quality in environment-friendly manner. The aim of this study was to determine the effect of an algae and bacteria preparations on the yield and nutritional parameters of leaf and romaine lettuce cultivated for spring and summer crop. The application of a combined biostimulant consisting of plant growth-promoting bacteria (Bacillus licheniformis, Bacillus megatherium, Azotobacter sp., Azospirillum sp., and Herbaspirillum sp.) and fresh water algae (Chlorella vulgaris) was done by watering the lettuce every 14 days, and a determination of the fresh weight, total antioxidant capacity, and total carotenoids content was performed. The result revealed that the application of bacterial-algal preparation significantly affected the plant weight of both romaine and leaf lettuce in spring and summer seasons. The highest increase in the weight of romaine lettuce reached 18.9% in the spring crop, while in the case of leaf lettuce, biostimulant treatment led to a 22.7% higher weight in the summer crop. Total antioxidant capacity and total carotenoids content showed increased values in the summer crop of romaine lettuce, while for the leaf lettuce there were no differences between treatments. Therefore, the positive effect of bacterial-algal treatment on lettuce yield, total antioxidant capacity and total carotenoids confirm that it could be applied for improving romaine lettuce yield quality and quantity, especially in stress, summer conditions.

16 March 2020 in Plants
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TKTomáš Kopta
Tomáš Kopta
Agnieszka SękaraAgnieszka Sękara
Agnieszka Sękara
RPRobert Pokluda
Robert Pokluda
VFVojtech Ferby
Vojtech Ferby
Gianluca CarusoGianluca Caruso
Gianluca Caruso
In many regions of the world, the production of vegetable crops is limited by a deepening water crisis. Drought stress affects productivity and the chemical composition of crops. The variability of drought tolerance between species and cultivars of economically important crops, such as pepper (Capsicum species), requires specific investigations to understand the physiological and biochemical responses to the aftermath of drought. The fruits and leaves of four chilli pepper cultivars were investigated to elucidate the fruits’ pungency (Scoville Heat Units, SHU), ascorbic acid content, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, polyphenol content, membrane lipid peroxidation and key protective antioxidant enzyme activity under drought stress (18–28% volumetric water content) as compared to the control (35–60%). Drought increased the chilli pepper fruits’ pungency expressed in Scoville Heat Units (SHU) as well as ascorbic acid content, but this relationship was also dependent on genotype and stress duration. ‘Jolokia’ was marked as most sensitive to drought by increasing content of capsaicinoids and DPPH˙ scavenging activity under stress conditions. Capsaicinoids and Ascorbic acid (AsA) greatly influenced the antioxidant activity of highly pungent chilli pepper fruits, although total phenols played a significant role in the mildly pungent genotypes. Generally, the activities of antioxidant enzymes increased under drought in chilli pepper leaves and fruits, although the intensity of the reaction varied among the cultivars used in the current research. All the investigated biochemical parameters were involved in the drought response of chilli pepper plants, but their significance and effectiveness were highly cultivar-dependent.

1 August 2016 in Horticulture, Environment, and Biotechnology
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AKAndrzej Kalisz
Andrzej Kalisz
AJAleš Jezdinský
Aleš Jezdinský
RPRobert Pokluda
Robert Pokluda
Agnieszka SękaraAgnieszka Sękara
Agnieszka Sękara
AGAneta Grabowska
Aneta Grabowska
JGJoanna Gil
Joanna Gil

7 December 2022 in Plants
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SSSana Saleem
Sana Saleem
Agnieszka SękaraAgnieszka Sękara
Agnieszka Sękara
RPRobert Pokluda
Robert Pokluda
Fulfilling the food demand of a fast-growing population is a global concern, resulting in increased dependence of the agricultural sector on various chemical formulations for enhancing crop production. This leads to an overuse of chemicals, which is not only harmful to human and animal health, but also to the environment and the global economy. Environmental safety and sustainable production are major responsibilities of the agricultural sector, which is inherently linked to the conservation of the biodiversity, the economy, and human and animal health. Scientists, therefore, across the globe are seeking to develop eco-friendly and cost-effective strategies to mitigate these issues by putting more emphasis on the use of beneficial microorganisms. Here, we review the literature on Serendipita indica, a beneficial endophytic fungus, to bring to the fore its properties of cultivation, the ability to enhance plant growth, improve the quality of produced crops, mitigate various plant stresses, as well as protect the environment. The major points in this review are as follows: (1) Although various plant growth promoting microorganisms are available, the distinguishing character of S. indica being axenically cultivable with a wide range of hosts makes it more interesting for research. (2) S. indica has numerous functions, ranging from promoting plant growth and quality to alleviating abiotic and biotic stresses, suggesting the use of this fungus as a biofertiliser. It also improves the soil quality by limiting the movement of heavy metals in the soil, thus, protecting the environment. (3) S. indica’s modes of action are due to interactions with phytohormones, metabolites, photosynthates, and gene regulation, in addition to enhancing nutrient and water absorption. (4) Combined application of S. indica and nanoparticles showed synergistic promotion in crop growth, but the beneficial effects of these interactions require further investigation. This review concluded that S. indica has a great potential to be used as a plant growth promoter or biofertiliser, ensuring sustainable crop production and a healthy environment.

20 May 2020 in PLOS ONE
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ABAkila Berraf-Tebbal
Akila Berraf-Tebbal
AMAlla Eddine Mahamedi
Alla Eddine Mahamedi
WAWassila Aigoun-Mouhous
Wassila Aigoun-Mouhous
MSMilan Spetik
Milan Spetik
JČJana Čechová
Jana Čechová
Artur AlvesArtur Alves
Artur Alves
Several Botryosphaeriaceae species are known to occur worldwide, causing dieback, canker and fruit rot on various hosts. Surveys conducted in ten commercial citrus orchards in the northern region of Algeria revealed five species of Botryosphaeriaceae belonging to three genera associated with diseased trees. Morphological and cultural characteristics as well as phylogenetic analyses of the internal transcribed spacer (ITS) region and the translation elongation factor 1-alpha (tef1-α) identified Diplodia mutila, Diplodia seriata, Dothiorella viticola, Lasiodiplodia mediterranea and a novel species which is here described as Lasiodiplodia mithidjana sp. nov.. Of these, L. mithidjana (14.1% of the samples) and L. mediterranea (13% of the samples) were the most widespread and abundant species. Pathogenicity tests revealed that L. mediterranea and D. seriata were the most aggressive species on citrus shoots. This study highlights the importance of Botryosphaeriaceae species as agents of canker and dieback of citrus trees in Algeria.