The ability to manipulate dense micrometre-scale objects in fluids is of interest to biosciences with a view to improving analysis techniques and enabling tissue engineering. A method of trapping micrometre-scale particles and manipulating them on a two-dimensional plane is proposed and demonstrated. Phase-controlled counter-propagating waves are used to generate ultrasonic standing waves with arbitrary nodal positions. The acoustic radiation force drives dense particles to pressure nodes. It is shown analytically that a series of point-like traps can be produced in a two-dimensional plane using two orthogonal pairs of counter-propagating waves. These traps can be manipulated by appropriate adjustment of the relative phases. Four 5 MHz transducers (designed to minimize reflection) are used as sources of counter-propagating waves in a water-filled cavity. Polystyrene beads of 10 μm diameter are trapped and manipulated. The relationship between trapped particle positions and the relative phases of the four transducers is measured and shown to agree with analytically derived expressions. The force available is measured by determining the response to a sudden change in field and found to be 30 pN, for a 30 V _pp input, which is in agreement with the predictions of models of the system. A scalable fabrication approach to producing devices is demonstrated.

We demonstrate that acoustic levitation can levitate spherical objects much larger than the acoustic wavelength in air. The acoustic levitation of an expanded polystyrene sphere of 50 mm in diameter, corresponding to 3.6 times the wavelength, is achieved by using three 25 kHz ultrasonic transducers arranged in a tripod fashion. In this configuration, a standing wave is created between the transducers and the sphere. The axial acoustic radiation force generated by each transducer on the sphere was modeled numerically as a function of the distance between the sphere and the transducer. The theoretical acoustic radiation force was verified experimentally in a setup consisting of an electronic scale and an ultrasonic transducer mounted on a motorized linear stage. The comparison between the numerical and experimental acoustic radiation forces presents a good agreement.

Accurate control over positioning of cells is a highly desirable feature in tissue engineering applications since it allows, for example, population of substrates in a controlled fashion, rather than relying on random seeding. Current methods to achieve a differential distribution of cells mostly use passive patterning methods to change chemical, mechanical or topographic properties of surfaces, making areas differentially permissive to the adhesion of cells. However, these methods have no ad hoc control over the actual deposition of cells. Direct patterning methods like bioprinting offer good control over cell position, but require sophisticated instrumentation and are often cost- and time-intensive. Here, we present a novel electronically controlled method of generating dynamic cell patterns by acoustic trapping of cells at a user-determined position, with a heptagonal acoustic tweezer device. We demonstrate the capability of the device to create complex patterns of cells using the device's ability to re-position acoustic traps by using a phase shift in the acoustic wave, and by switching the configuration of active piezoelectric transducers. Furthermore, we show that by arranging Schwann cells from neonatal rats in a linear pattern we are able to create Bands of Büngner-like structures on a non-structured surface and demonstrate that these features are able to guide neurite outgrowth from neonatal rat dorsal root ganglia.

Levitation and manipulation of objects by sound waves have a wide range of applications in chemistry, biology, material sciences, and engineering. However, the current acoustic levitation techniques are mainly restricted to particles that are much smaller than the acoustic wavelength. In this work, it is shown that acoustic standing waves can be employed to stably levitate an object much larger than the acoustic wavelength in air. The levitation of a large slightly curved object weighting 2.3 g is demonstrated by using a device formed by two 25 kHz ultrasonic Langevin transducers connected to an aluminum plate. The sound wave emitted by the device provides a vertical acoustic radiation force to counteract gravity and a lateral restoring force that ensure horizontal stability to the levitated object. In order to understand the levitation stability, a numerical model based on the finite element method is used to determine the acoustic radiation force that acts on the object.

An ultrasonic device for micro-patterning and precision manipulation of micrometre-scale particles is demonstrated. The device is formed using eight piezoelectric transducers shaped into an octagonal cavity. By exciting combinations of transducers simultaneously, with a controlled phase delay between them, different acoustic landscapes can be created, patterning micro-particles into lines, squares, and more complex shapes. When operated with all eight transducers the device can, with appropriate phase control, manipulate the two dimensional acoustic pressure gradient; it thus has the ability to position and translate a single tweezing zone to different locations on a surface in a precise and programmable manner.