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It is well known that S interacts with some macronutrients, such as N, P, and K, as well as with some micronutrients, such as Fe, Mo, Cu, Zn, and B. From our current understanding, such interactions could be related to the fact that: (i) S shares similar chemical properties with other elements (e.g., Mo and Se) determining competition for the acquisition/transport process (SULTR transporter family proteins); (ii) S-requiring metabolic processes need the presence of other nutrients or regulate plant responses to other nutritional deficiencies (S-containing metabolites are the precursor for the synthesis of ethylene and phytosiderophores); (iii) S directly interacts with other elements (e.g., Fe) by forming complexes and chemical bonds, such as Fe-S clusters; and (iv) S is a constituent of organic molecules, which play crucial roles in plants (glutathione, transporters, etc.). This review summarizes the current state of knowledge of the interplay between Fe and S in plants. It has been demonstrated that plant capability to take up and accumulate Fe strongly depends on S availability in the growth medium in both monocots and dicot plants. Moreover, providing S above the average nutritional need enhances the Fe content in wheat grains, this beneficial effect being particularly pronounced under severe Fe limitation. On the other hand, Fe shortage induces a significant increase in the demand for S, resulting in enhanced S uptake and assimilation rate, similar to what happens under S deficiency. The critical evaluation of the recent studies on the modulation of Fe/S interaction by integrating old and new insights gained on this topic will help to identify the main knowledge gaps. Indeed, it remains a challenge to determine how the interplay between S and Fe is regulated and how plants are able to sense environmental nutrient fluctuations and then to adapt their uptake, translocation, assimilation, and signaling. A better knowledge of the mechanisms of Fe/S interaction might considerably help in improving crop performance within a context of limited nutrient resources and a more sustainable agriculture.
Stefania Astolfi; Silvia Celletti; Gianpiero Vigani; Tanja Mimmo; Stefano Cesco. Interaction Between Sulfur and Iron in Plants. 2021, 12, 1 .
AMA StyleStefania Astolfi, Silvia Celletti, Gianpiero Vigani, Tanja Mimmo, Stefano Cesco. Interaction Between Sulfur and Iron in Plants. . 2021; 12 ():1.
Chicago/Turabian StyleStefania Astolfi; Silvia Celletti; Gianpiero Vigani; Tanja Mimmo; Stefano Cesco. 2021. "Interaction Between Sulfur and Iron in Plants." 12, no. : 1.
Improving the agronomic use of recycled nutrients derived from organic waste is one of the priorities within the measures adopted by the European community to reduce environmental issues but remains an unexplored area of research. This study focused on investigating the possibility of using innovative fertilizer solutions in hydroponic systems for the growth of agricultural plants. To this purpose, a liquid fraction [aqueous hydrothermal carbonization (HTC) liquid (AHL)] derived from HTC of cow manure digestate was chemically characterized (pH, electrical conductivity, mineral elements, and organic compounds such as phytotoxins), diluted with distilled water (1:30, 1:60, and 1:90, v/v) to reduce its potential phytotoxicity, and used to grow hydroponic maize (Zea mays L.) plants instead of the classical full-strength nutrient solution. The results indicated that the dilution ratio 1:30 of the AHL solution maintained a high level of toxicity for the plants (phytotoxic substances, especially Na and alkalinity), inducing the arrest of their growth. Differently, the two other dilution ratios (i.e., 1:60 and 1:90) seemed to considerably limit the levels of toxicity, since they allowed the plants to develop. However, these dilution ratios were poor in nutrient elements, inducing alteration in photosynthesis and an onset of deficiency symptoms such as pronounced leaf chlorosis. In view of an eco-friendly approach, future studies are, therefore, needed to identify the correct species-specific dilution ratio to supply both low levels of phytotoxins and adequate content of essential nutrients for appropriate plant growth and development. Furthermore, in order to lower specific Na phytotoxicity, treatments are of utmost importance before using AHL as a fertilizer solution.
Silvia Celletti; Maximilian Lanz; Alex Bergamo; Vittoria Benedetti; Daniele Basso; Marco Baratieri; Stefano Cesco; Tanja Mimmo. Evaluating the Aqueous Phase From Hydrothermal Carbonization of Cow Manure Digestate as Possible Fertilizer Solution for Plant Growth. Frontiers in Plant Science 2021, 12, 1 .
AMA StyleSilvia Celletti, Maximilian Lanz, Alex Bergamo, Vittoria Benedetti, Daniele Basso, Marco Baratieri, Stefano Cesco, Tanja Mimmo. Evaluating the Aqueous Phase From Hydrothermal Carbonization of Cow Manure Digestate as Possible Fertilizer Solution for Plant Growth. Frontiers in Plant Science. 2021; 12 ():1.
Chicago/Turabian StyleSilvia Celletti; Maximilian Lanz; Alex Bergamo; Vittoria Benedetti; Daniele Basso; Marco Baratieri; Stefano Cesco; Tanja Mimmo. 2021. "Evaluating the Aqueous Phase From Hydrothermal Carbonization of Cow Manure Digestate as Possible Fertilizer Solution for Plant Growth." Frontiers in Plant Science 12, no. : 1.
Strawberry is one of the most popular fruits in the market. To meet the demanding consumer and market quality standards, there is a strong need for an on-site, accurate and reliable grading system during the whole harvesting process. In this work, a total of 923 strawberry fruit were measured directly on-plant at different ripening stages by means of bioimpedance data, collected at frequencies between 20 Hz and 300 kHz. The fruit batch was then splitted in 2 classes (i.e. ripe and unripe) based on surface color data. Starting from these data, six of the most commonly used supervised machine learning classification techniques, i.e. Logistic Regression (LR), Binary Decision Trees (DT), Naive Bayes Classifiers (NBC), K-Nearest Neighbors (KNN), Support Vector Machine (SVM) and Multi-Layer Perceptron Networks (MLP), were employed, optimized, tested and compared in view of their performance in predicting the strawberry fruit ripening stage. Such models were trained to develop a complete feature selection and optimization pipeline, not yet available for bioimpedance data analysis of fruit. The classification results highlighted that, among all the tested methods, MLP networks had the best performances on the test set, with 0.72, 0.82 and 0.73 for the F $$_1$$ 1 , F $$_{0.5}$$ 0.5 and F $$_2$$ 2 -score, respectively, and improved the training results, showing good generalization capability, adapting well to new, previously unseen data. Consequently, the MLP models, trained with bioimpedance data, are a promising alternative for real-time estimation of strawberry ripeness directly on-field, which could be a potential application technique for evaluating the harvesting time management for farmers and producers.
Pietro Ibba; Christian Tronstad; Roberto Moscetti; Tanja Mimmo; Giuseppe Cantarella; Luisa Petti; Ørjan G. Martinsen; Stefano Cesco; Paolo Lugli. Supervised binary classification methods for strawberry ripeness discrimination from bioimpedance data. Scientific Reports 2021, 11, 1 -13.
AMA StylePietro Ibba, Christian Tronstad, Roberto Moscetti, Tanja Mimmo, Giuseppe Cantarella, Luisa Petti, Ørjan G. Martinsen, Stefano Cesco, Paolo Lugli. Supervised binary classification methods for strawberry ripeness discrimination from bioimpedance data. Scientific Reports. 2021; 11 (1):1-13.
Chicago/Turabian StylePietro Ibba; Christian Tronstad; Roberto Moscetti; Tanja Mimmo; Giuseppe Cantarella; Luisa Petti; Ørjan G. Martinsen; Stefano Cesco; Paolo Lugli. 2021. "Supervised binary classification methods for strawberry ripeness discrimination from bioimpedance data." Scientific Reports 11, no. 1: 1-13.
A Corrigendum on Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots by Tiziani, R., Mimmo, T., Valentinuzzi, F., Pii, Y., Celletti, S., and Cesco, S. (2020). Front. Plant Sci. 11:584568. doi: 10.3389/fpls.2020.584568 In the original article, the authors neglected to acknowledge the funding provided by “Stiftung Südtiroler Sparkasse” (to Raphael Tiziani). The Funding section in the published article has been updated as presented below. This work was supported by grants from the Free University of Bolzano (NUMICS TN200E). “The doctoral fellowship of RT was funded by Stiftung Südtiroler Sparkasse.” The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated. Keywords: carboxylates, cluster roots, phosphate uptake, root exudates, white lupin Citation: Tiziani R, Mimmo T, Valentinuzzi F, Pii Y, Celletti S and Cesco S (2021) Corrigendum: Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots. Front. Plant Sci. 12:681263. doi: 10.3389/fpls.2021.681263 Received: 16 March 2021; Accepted: 16 March 2021; Published: 22 April 2021. Approved by: Copyright © 2021 Tiziani, Mimmo, Valentinuzzi, Pii, Celletti and Cesco. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. *Correspondence: Raphael Tiziani, [email protected]; Tanja Mimmo, [email protected]
Raphael Tiziani; Tanja Mimmo; Fabio Valentinuzzi; Youry Pii; Silvia Celletti; Stefano Cesco. Corrigendum: Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots. Frontiers in Plant Science 2021, 12, 681263 .
AMA StyleRaphael Tiziani, Tanja Mimmo, Fabio Valentinuzzi, Youry Pii, Silvia Celletti, Stefano Cesco. Corrigendum: Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots. Frontiers in Plant Science. 2021; 12 ():681263.
Chicago/Turabian StyleRaphael Tiziani; Tanja Mimmo; Fabio Valentinuzzi; Youry Pii; Silvia Celletti; Stefano Cesco. 2021. "Corrigendum: Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots." Frontiers in Plant Science 12, no. : 681263.
Plant Growth Promoting Rhizobacteria (PGPR) represent a heterogeneous group of bacteria, which have been characterized for their ability to influence the growth and the fitness of agricultural plants. In the quest of more sustainable practices, PGPR have been suggested as a valid complement for the agronomical practices, since they can influence several biochemical and molecular mechanisms related to the mineral nutrients uptake, the plant pathogens suppression, and the phytohormones production. Within the present work, three bacterial strains, namely Enterobacter asburiae BFD160, Pseudomonas koreensis TFD26, and Pseudomonas lini BFS112, previously characterized on the basis of distinctive PGPR traits, were tested to evaluate: (i) their persistence in soil microcosms; (ii) their effects on seeds germination; (iii) their possible influence on biochemical and physiological parameters related to plant growth, fruit quality, and plant nutrient acquisition and allocation. To these aims, two microcosms experiments featuring different complexities, i.e., namely a growth chamber and a tunnel, were used to compare the effects of the microbial inoculum to those of chemical fertilization on Cucumis sativus L. plants. In the growth experiment, the Pseudomonas spp. induced positive effects on both growth and physiological parameters; TFD26, in particular, induced an enhanced accumulation of mineral nutrients (Fe, Ca, Mn, Ni, Zn) in plant tissues. In the tunnel experiment, only P. koreensis TFD26 was selected as inoculum for cucumber plants used in combination or in alternative to a chemical fertilizer. Interestingly, the inoculation with TFD26 alone or in combination with half-strength chemical fertilizer could induce similar (e.g., Ca accumulation) or enhanced (e.g., micronutrients concentration in plant tissues and fruits) effects as compared to plants treated with full-strength chemical fertilizers. Overall, the results hereby presented show that the use of PGPR can lead to comparable, and in some cases improved, effects on biochemical and physiological parameters of cucumber plants and fruits. Although these data are referred to experiments carried out in controlled condition, though different from an open filed cultivation, our observations suggest that the application of PGPR and fertilizers mixtures might help shrinking the use of chemical fertilization and potentially leading to a more sustainable agricultural practice.
Marina Scagliola; Fabio Valentinuzzi; Tanja Mimmo; Stefano Cesco; Carmine Crecchio; Youry Pii. Bioinoculants as Promising Complement of Chemical Fertilizers for a More Sustainable Agricultural Practice. Frontiers in Sustainable Food Systems 2021, 4, 1 .
AMA StyleMarina Scagliola, Fabio Valentinuzzi, Tanja Mimmo, Stefano Cesco, Carmine Crecchio, Youry Pii. Bioinoculants as Promising Complement of Chemical Fertilizers for a More Sustainable Agricultural Practice. Frontiers in Sustainable Food Systems. 2021; 4 ():1.
Chicago/Turabian StyleMarina Scagliola; Fabio Valentinuzzi; Tanja Mimmo; Stefano Cesco; Carmine Crecchio; Youry Pii. 2021. "Bioinoculants as Promising Complement of Chemical Fertilizers for a More Sustainable Agricultural Practice." Frontiers in Sustainable Food Systems 4, no. : 1.
In recent decades, agriculture has faced the fundamental challenge of needing to increase food production and quality in order to meet the requirements of a growing global population. Similarly, viticulture has also been undergoing change. Several countries are reducing their vineyard areas, and several others are increasing them. In addition, viticulture is moving towards higher altitudes and latitudes due to climate change. Furthermore, global warming is also exacerbating the incidence of fungal diseases in vineyards, forcing farmers to apply agrochemicals to preserve production yields and quality. The repeated application of copper (Cu)-based fungicides in conventional and organic farming has caused a stepwise accumulation of Cu in vineyard soils, posing environmental and toxicological threats. High Cu concentrations in soils can have multiple impacts on agricultural systems. In fact, it can (i) alter the chemical-physical properties of soils, thus compromising their fertility; (ii) induce toxicity phenomena in plants, producing detrimental effects on growth and productivity; and (iii) affect the microbial biodiversity of soils, thereby influencing some microbial-driven soil processes. However, several indirect (e.g., management of rhizosphere processes through intercropping and/or fertilization strategies) and direct (e.g., exploitation of vine resistant genotypes) strategies have been proposed to restrain Cu accumulation in soils. Furthermore, the application of precision and smart viticulture paradigms and their related technologies could allow a timely, localized and balanced distribution of agrochemicals to achieve the required goals. The present review highlights the necessity of applying multidisciplinary approaches to meet the requisites of sustainability demanded of modern viticulture.
Stefano Cesco; Youry Pii; Luigimaria Borruso; Guido Orzes; Paolo Lugli; Fabrizio Mazzetto; Giulio Genova; Marco Signorini; Gustavo Brunetto; Roberto Terzano; Gianpiero Vigani; Tanja Mimmo. A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity. Applied Sciences 2021, 11, 907 .
AMA StyleStefano Cesco, Youry Pii, Luigimaria Borruso, Guido Orzes, Paolo Lugli, Fabrizio Mazzetto, Giulio Genova, Marco Signorini, Gustavo Brunetto, Roberto Terzano, Gianpiero Vigani, Tanja Mimmo. A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity. Applied Sciences. 2021; 11 (3):907.
Chicago/Turabian StyleStefano Cesco; Youry Pii; Luigimaria Borruso; Guido Orzes; Paolo Lugli; Fabrizio Mazzetto; Giulio Genova; Marco Signorini; Gustavo Brunetto; Roberto Terzano; Gianpiero Vigani; Tanja Mimmo. 2021. "A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity." Applied Sciences 11, no. 3: 907.
Here, we investigated the possible linkages among geophagy, soil characteristics, and gut mycobiome of indri (Indri indri), an endangered lemur species able to survive only in wild conditions. The soil eaten by indri resulted in enriched secondary oxide-hydroxides and clays, together with a high concentration of specific essential micronutrients. This could partially explain the role of the soil in detoxification and as a nutrient supply. Besides, we found that soil subject to geophagy and indris’ faeces shared about 8.9% of the fungal OTUs. Also, several genera (e.g. Fusarium, Aspergillus and Penicillium) commonly associated with soil and plant material were found in both geophagic soil and indri samples. On the contrary, some taxa with pathogenic potentials, such as Cryptococcus, were only found in indri samples. Further, many saprotrophs and plant-associated fungal taxa were detected in the indri faeces. These fungal species may be involved in the digestion processes of leaves and could have a beneficial role in their health. In conclusion, we found an intimate connection between gut mycobiome and soil, highlighting, once again, the potential consequent impacts on the wider habitat.
Luigimaria Borruso; Alice Checcucci; Valeria Torti; Federico Correa; Camillo Sandri; Daine Luise; Luciano Cavani; Monica Modesto; Caterina Spiezio; Tanja Mimmo; Stefano Cesco; Maura Di Vito; Francesca Bugli; Rose M. Randrianarison; Marco Gamba; Nianja J. Rarojoson; Cesare Avesani Zaborra; Paola Mattarelli; Paolo Trevisi; Cristina Giacoma. I Like the Way You Eat It: Lemur (Indri indri) Gut Mycobiome and Geophagy. Microbial Ecology 2021, 82, 1 -9.
AMA StyleLuigimaria Borruso, Alice Checcucci, Valeria Torti, Federico Correa, Camillo Sandri, Daine Luise, Luciano Cavani, Monica Modesto, Caterina Spiezio, Tanja Mimmo, Stefano Cesco, Maura Di Vito, Francesca Bugli, Rose M. Randrianarison, Marco Gamba, Nianja J. Rarojoson, Cesare Avesani Zaborra, Paola Mattarelli, Paolo Trevisi, Cristina Giacoma. I Like the Way You Eat It: Lemur (Indri indri) Gut Mycobiome and Geophagy. Microbial Ecology. 2021; 82 (1):1-9.
Chicago/Turabian StyleLuigimaria Borruso; Alice Checcucci; Valeria Torti; Federico Correa; Camillo Sandri; Daine Luise; Luciano Cavani; Monica Modesto; Caterina Spiezio; Tanja Mimmo; Stefano Cesco; Maura Di Vito; Francesca Bugli; Rose M. Randrianarison; Marco Gamba; Nianja J. Rarojoson; Cesare Avesani Zaborra; Paola Mattarelli; Paolo Trevisi; Cristina Giacoma. 2021. "I Like the Way You Eat It: Lemur (Indri indri) Gut Mycobiome and Geophagy." Microbial Ecology 82, no. 1: 1-9.
Contaminated soils are lands in Europe deemed less favourable for conventional agriculture. To overcome the problem of their poor fertility, bio-fertilization could be a promising approach. Soil inoculation with a choice of biological species (e.g. earthworm, mycorrhizal fungi, diazotroph bacteria) can be performed in order to improve soil properties and promote nutrients recycling. However, questions arise concerning the dynamics of the contaminants in an inoculated soil. The aim of this study was to highlight the soil-plant-earthworm interactions in the case of a slightly contaminated soil. For this purpose, a pot experiment in controlled conditions was carried out during 2 months with a Cd, Zn, and Cu contaminated sandy soil, including conditions with or without earthworms (Aporrectodea caliginosa) and with or without plants (Lolium perenne). The three components of the trace element bioavailability were studied to understand the belowground-aboveground relationships and were quantified as followed: i) environmental availability in soils by measuring trace element concentrations in soil solution, ii) environmental bioavailability for organisms by measuring trace element concentrations in depurated whole earthworms bodies and in the plant aerial biomass, and iii) toxicological bioavailability, by measuring survival rate and body weight changes for earthworms and biomass for plants. The results showed that earthworm inoculation increased the content of all studied TE in soil solution. Moreover, lower concentrations of Cd and Zn were found in plants in the presence of earthworms while the bioavailability decreased when compared to the condition without plants. The trace element bioaccumulation in earthworms did not produce a direct toxicity, according to the earthworm survival rate and body weight results. Finally, our pot experiment confirmed that even in contaminated soils, the presence of A. caliginosa promotes plant adaptation and improves biomass production, reducing trace element uptake.
Olivier Hullot; Isabelle Lamy; Raphael Tiziani; Tanja Mimmo; Lisa Ciadamidaro. The effect of earthworms on plant response in metal contaminated soil focusing on belowground-aboveground relationships. Environmental Pollution 2021, 274, 116499 .
AMA StyleOlivier Hullot, Isabelle Lamy, Raphael Tiziani, Tanja Mimmo, Lisa Ciadamidaro. The effect of earthworms on plant response in metal contaminated soil focusing on belowground-aboveground relationships. Environmental Pollution. 2021; 274 ():116499.
Chicago/Turabian StyleOlivier Hullot; Isabelle Lamy; Raphael Tiziani; Tanja Mimmo; Lisa Ciadamidaro. 2021. "The effect of earthworms on plant response in metal contaminated soil focusing on belowground-aboveground relationships." Environmental Pollution 274, no. : 116499.
The reliable sampling of root exudates in soil grown plants is experimentally challenging. This study aimed at developing a citrate sampling and mapping technique with millimetre-resolution using DGT (diffusive gradients in thin films) ZrOH binding gels. Citrate adsorption kinetics, DGT capacity and stability of ZrOH gels were evaluated. ZrOH gels were applied to generate 2D maps of citrate exuded by white lupin roots grown in rhizotrosn in a phosphorus deficient soil. Citrate was adsorbed quantitatively and rapidly by the ZrOH gels, these gels can be stored after sampling for several weeks prior to analysis. The DGT capacity of the ZrOH gel for citrate depends on the ionic strength and the pH of the soil solution but was suitable for citrate sampling. 2D citrate maps of rhizotron grown plants have been generated for the first time at a millimetre resolution to measure an illustrated plant response to P fertilization. DGT-based citrate sampling is suitable for studying the root exudation in soil environments, at unprecedented spatial resolution. By changing binding material, the technique is also applicable to other exudate classes and might be used for the evaluation of whole root exudation crucial in specific cultivar breeding. Highlight We present a novel, reliable, easy to use, non-destructive citrate sampling- and two-dimensional high-resolution imaging technique for soil grown plant roots.
Raphael Tiziani; Markus Puschenreiter; Erik Smolders; Tanja Mimmo; José Carlos Herrera; Stefano Cesco; Jakob Santner. Millimeter-resolution mapping of citrate exuded from soil grown roots using a novel, low-invasive sampling technique. 2020, 1 .
AMA StyleRaphael Tiziani, Markus Puschenreiter, Erik Smolders, Tanja Mimmo, José Carlos Herrera, Stefano Cesco, Jakob Santner. Millimeter-resolution mapping of citrate exuded from soil grown roots using a novel, low-invasive sampling technique. . 2020; ():1.
Chicago/Turabian StyleRaphael Tiziani; Markus Puschenreiter; Erik Smolders; Tanja Mimmo; José Carlos Herrera; Stefano Cesco; Jakob Santner. 2020. "Millimeter-resolution mapping of citrate exuded from soil grown roots using a novel, low-invasive sampling technique." , no. : 1.
Biostimulants play an important role in the development of management practices able to reach adequate productivity to meet the food demand of a growing world population, while following a sustainable agriculture model. This work aims to evaluate the effect of a protein hydrolysate derived from legume seeds by enzymatic hydrolysis on plant growth and also to verify its ability to mitigate Fe deficiency, a widespread problem significantly limiting plant growth and crop productivity. Experiments were performed with tomato (Solanum lycopersicum L.—cv. AKRAI F1) and cucumber (Cucumis sativus L.—cv. EKRON F1). The plants were grown hydroponically under adequate or limited Fe supply. Changes in shoot and root fresh weight, leaf relative chlorophyll content and the accumulation of macro- and microelements in shoots and roots were measured. Plant ability to cope with Fe deficiency was measured by evaluating the activity of root Fe3+-chelate reductase. Our results indicate that the foliar treatments with the protein hydrolysate did not significantly affect growth parameters when plants were grown in full nutrient solution. However, the biostimulant was able to improve the growth performance of Fe-deficient plants. Therefore, the protein hydrolysate can be a powerful tool to stimulate crop growth under Fe-deficient environments, leading to reduced fertilizer inputs with related environmental and economic benefits.
Silvia Celletti; Stefania Astolfi; Nicoletta Guglielmo; Giuseppe Colla; Stefano Cesco; Tanja Mimmo. Evaluation of a Legume-Derived Protein Hydrolysate to Mitigate Iron Deficiency in Plants. Agronomy 2020, 10, 1942 .
AMA StyleSilvia Celletti, Stefania Astolfi, Nicoletta Guglielmo, Giuseppe Colla, Stefano Cesco, Tanja Mimmo. Evaluation of a Legume-Derived Protein Hydrolysate to Mitigate Iron Deficiency in Plants. Agronomy. 2020; 10 (12):1942.
Chicago/Turabian StyleSilvia Celletti; Stefania Astolfi; Nicoletta Guglielmo; Giuseppe Colla; Stefano Cesco; Tanja Mimmo. 2020. "Evaluation of a Legume-Derived Protein Hydrolysate to Mitigate Iron Deficiency in Plants." Agronomy 10, no. 12: 1942.
Microbial communities proliferating at the root-soil interface, collectively referred to as the rhizosphere microbiota, represent an untapped beneficial resource for plant growth, development and health. Integral to a rational manipulation of the microbiota for sustainable agriculture is the identification of the molecular determinants of these communities. In plants, biosynthesis of allelochemicals is centre stage in defining inter-organismal relationships in the environment. Intriguingly, this process has been moulded by domestication and breeding selection. The indole-alkaloid gramine, whose occurrence in barley (Hordeum vulgare L.) is widespread among wild genotypes but has been counter selected in several modern varieties, is a paradigmatic example of this phenomenon. This prompted us to investigate how exogenous applications of gramine impacted on the rhizosphere microbiota of two, gramine-free, elite barley varieties grown in a reference agricultural soil. High throughput 16S rRNA gene amplicon sequencing revealed that applications of gramine interfere with the proliferation of a subset of soil microbes with a relatively broad phylogenetic assignment. Strikingly, growth of these bacteria appeared to be rescued by barley plants in a genotype- and dosage-independent manner. In parallel, we discovered that host recruitment cues can interfere with the impact of gramine application in a host genotype-dependent manner. Interestingly, this latter effect displayed a bias for members of the phyla Proteobacteria. These initial observations indicate that gramine can act as a determinant of the prokaryotic communities inhabiting the root-soil interface.
Mauro Maver; Carmen Escudero-Martinez; James Abbott; Jenny Morris; Pete E. Hedley; Tanja Mimmo; Davide Bulgarelli. Applications of the indole-alkaloid gramine shape the prokaryotic microbiota thriving at the barley root-soil interface. 2020, 1 .
AMA StyleMauro Maver, Carmen Escudero-Martinez, James Abbott, Jenny Morris, Pete E. Hedley, Tanja Mimmo, Davide Bulgarelli. Applications of the indole-alkaloid gramine shape the prokaryotic microbiota thriving at the barley root-soil interface. . 2020; ():1.
Chicago/Turabian StyleMauro Maver; Carmen Escudero-Martinez; James Abbott; Jenny Morris; Pete E. Hedley; Tanja Mimmo; Davide Bulgarelli. 2020. "Applications of the indole-alkaloid gramine shape the prokaryotic microbiota thriving at the barley root-soil interface." , no. : 1.
The management of digestate, the main by-product of the anaerobic digestion (AD) process, is one of the most serious environmental issues. Although digestate is used on arable land as a fertilizer, it can have a negative impact on the environment due to nitrate leaching into the groundwater and ammonia volatilization into the atmosphere, with high economic and environmental disposal costs. Therefore, hydrothermal carbonization (HTC), a thermochemical biomass conversion process, could represent a sustainable and efficient alternative for digestate management. Hydrochar, the solid product of the HTC process, has been recently proposed as a plant growing medium in soilless culture systems (SCS). Here, using cow manure digestate as feedstock, we investigated the influence of the HTC process reaction temperature (180, 220 and 250 °C) and residence time (1 and 3 h) on the physical-chemical properties (pH, electrical conductivity, and mineral element concentrations) of the resulting hydrochars. Furthermore, in order to fully valorize hydrochar as a growing medium, their possible phytotoxic effects and those of their water extracts (prepared at two different concentrations and at different pHs) were tested in germination tests with cress seeds (Lepidium sativum L.). Concentrations of nutrients, heavy metals, organic acids, sugars and furan compounds were determined in the water extracts. Characterization analysis of these hydrochars revealed that they can be distinguished from each other by their physical-chemical properties, which were significantly affected by the two process parameters. Specifically, the HTC temperature had a greater effect on the composition of hydrochars than the residence time. Germination tests found hydrochar water extracts to show significantly lower phytotoxicity than the hydrochars themselves. Notably, the phytotoxic effect of the extracts decreased with increasing extraction ratio and decreasing pH. The chromatographic characterization of extracts identified the presence of potential phytotoxins, such as furan compounds (i.e., hydroxymethylfurfural and furfural). However, before using hydrochars as potential and innovative growing media for plants, their phytotoxicity should be limited, for example through their dilution with other substrates. Overall, AD-HTC coupling could represent a valuable eco-sustainable expedient in the field of biomasses, green economy and waste conversion and, therefore, further investigations in this direction are necessary.
Silvia Celletti; Alex Bergamo; Vittoria Benedetti; Matteo Pecchi; Francesco Patuzzi; Daniele Basso; Marco Baratieri; Stefano Cesco; Tanja Mimmo. Phytotoxicity of hydrochars obtained by hydrothermal carbonization of manure-based digestate. Journal of Environmental Management 2020, 280, 111635 .
AMA StyleSilvia Celletti, Alex Bergamo, Vittoria Benedetti, Matteo Pecchi, Francesco Patuzzi, Daniele Basso, Marco Baratieri, Stefano Cesco, Tanja Mimmo. Phytotoxicity of hydrochars obtained by hydrothermal carbonization of manure-based digestate. Journal of Environmental Management. 2020; 280 ():111635.
Chicago/Turabian StyleSilvia Celletti; Alex Bergamo; Vittoria Benedetti; Matteo Pecchi; Francesco Patuzzi; Daniele Basso; Marco Baratieri; Stefano Cesco; Tanja Mimmo. 2020. "Phytotoxicity of hydrochars obtained by hydrothermal carbonization of manure-based digestate." Journal of Environmental Management 280, no. : 111635.
Tree responses to fertilizer management are complex and are influenced by the interactions between the environment, other organisms, and the combined genetics of composite trees. Increased consumer awareness of the environmental impact of agriculture has stimulated research toward increasing nutrient-use efficiency, improving environmental sustainability, and maximizing quality. Here, we highlight recent advancements and identify knowledge gaps in nutrient dynamics across the soil–rhizosphere–tree continuum for fruit crops. Beneficial soil management practices can enhance nutrient uptake and there has been significant progress in the understanding of how roots, microorganisms, and soil interact to enhance nutrient acquisition in the rhizosphere. Characterizing root architecture, in situ, still remains one of the greatest research challenges in perennial fruit research. However, the last decade has advanced the characterization of root nutrient uptake and transport in plants but studies in tree fruit crops have been limited. Calcium, and its balance relative to other macronutrients, has been a primary focus for mineral nutrient research because of its important contributions to the development of physiological disorders. However, annual elemental redistribution makes these interactions complex. The development of new approaches for measuring nutrient movement in soil and plant systems will be critical for achieving sustainable production of high-quality fruit in the future.
Lee Kalcsits; Elmi Lotze; Massimo Tagliavini; Kirsten Hannam; Tanja Mimmo; Denise Neilsen; Gerry Neilsen; David Atkinson; Erica Casagrande Biasuz; Luigimaria Borruso; Stefano Cesco; Esmaeil Fallahi; Youry Pii; Nadia Valverdi. Recent Achievements and New Research Opportunities for Optimizing Macronutrient Availability, Acquisition, and Distribution for Perennial Fruit Crops. Agronomy 2020, 10, 1738 .
AMA StyleLee Kalcsits, Elmi Lotze, Massimo Tagliavini, Kirsten Hannam, Tanja Mimmo, Denise Neilsen, Gerry Neilsen, David Atkinson, Erica Casagrande Biasuz, Luigimaria Borruso, Stefano Cesco, Esmaeil Fallahi, Youry Pii, Nadia Valverdi. Recent Achievements and New Research Opportunities for Optimizing Macronutrient Availability, Acquisition, and Distribution for Perennial Fruit Crops. Agronomy. 2020; 10 (11):1738.
Chicago/Turabian StyleLee Kalcsits; Elmi Lotze; Massimo Tagliavini; Kirsten Hannam; Tanja Mimmo; Denise Neilsen; Gerry Neilsen; David Atkinson; Erica Casagrande Biasuz; Luigimaria Borruso; Stefano Cesco; Esmaeil Fallahi; Youry Pii; Nadia Valverdi. 2020. "Recent Achievements and New Research Opportunities for Optimizing Macronutrient Availability, Acquisition, and Distribution for Perennial Fruit Crops." Agronomy 10, no. 11: 1738.
Sweet basil (Ocimum basilicum L.) is one of the most produced aromatic herbs in the world, exploiting hydroponic systems. It has been widely assessed that macronutrients, like nitrogen (N) and sulfur (S), can strongly affect the organoleptic qualities of agricultural products, thus influencing their nutraceutical value. In addition, plant-growth-promoting rhizobacteria (PGPR) have been shown to affect plant growth and quality. Azospirillum brasilense is a PGPR able to colonize the root system of different crops, promoting their growth and development and influencing the acquisition of mineral nutrients. On the bases of these observations, we aimed at investigating the impact of both mineral nutrients supply and rhizobacteria inoculation on the nutraceutical value on two different sweet basil varieties, i.e., Genovese and Red Rubin. To these objectives, basil plants have been grown in hydroponics, with nutrient solutions fortified for the concentration of either S or N, supplied as SO42– or NO3–, respectively. In addition, plants were either non-inoculated or inoculated with A. brasilense. At harvest, basil plants were assessed for the yield and the nutraceutical properties of the edible parts. The cultivation of basil plants in the fortified nutrient solutions showed a general increasing trend in the accumulation of the fresh biomass, albeit the inoculation with A. brasilense did not further promote the growth. The metabolomic analyses disclosed a strong effect of treatments on the differential accumulation of metabolites in basil leaves, producing the modulation of more than 400 compounds belonging to the secondary metabolism, as phenylpropanoids, isoprenoids, alkaloids, several flavonoids, and terpenoids. The primary metabolism that resulted was also influenced by the treatments showing changes in the fatty acid, carbohydrates, and amino acids metabolism. The amino acid analysis revealed that the treatments induced an increase in arginine (Arg) content in the leaves, which has been shown to have beneficial effects on human health. In conclusion, between the two cultivars studied, Red Rubin displayed the most positive effect in terms of nutritional value, which was further enhanced following A. brasilense inoculation.
Simun Kolega; Begona Miras-Moreno; Valentina Buffagni; Luigi Lucini; Fabio Valentinuzzi; Mauro Maver; Tanja Mimmo; Marco Trevisan; Youry Pii; Stefano Cesco. Nutraceutical Profiles of Two Hydroponically Grown Sweet Basil Cultivars as Affected by the Composition of the Nutrient Solution and the Inoculation With Azospirillum brasilense. Frontiers in Plant Science 2020, 11, 1 .
AMA StyleSimun Kolega, Begona Miras-Moreno, Valentina Buffagni, Luigi Lucini, Fabio Valentinuzzi, Mauro Maver, Tanja Mimmo, Marco Trevisan, Youry Pii, Stefano Cesco. Nutraceutical Profiles of Two Hydroponically Grown Sweet Basil Cultivars as Affected by the Composition of the Nutrient Solution and the Inoculation With Azospirillum brasilense. Frontiers in Plant Science. 2020; 11 ():1.
Chicago/Turabian StyleSimun Kolega; Begona Miras-Moreno; Valentina Buffagni; Luigi Lucini; Fabio Valentinuzzi; Mauro Maver; Tanja Mimmo; Marco Trevisan; Youry Pii; Stefano Cesco. 2020. "Nutraceutical Profiles of Two Hydroponically Grown Sweet Basil Cultivars as Affected by the Composition of the Nutrient Solution and the Inoculation With Azospirillum brasilense." Frontiers in Plant Science 11, no. : 1.
Plasmopara viticola is one of the most important pathogens infecting Vitis vinifera plants. The interactions among P. viticola and both susceptible and resistant grapevine plants have been extensively characterised, at transcriptomic, proteomic and metabolomic levels. However, the involvement of plants ionome in the response against the pathogen has been completely neglected so far. Therefore, this study was aimed at investigating the possible role of leaf ionomic modulation during compatible and incompatible interactions between P. viticola and grapevine plants. In susceptible cultivars, a dramatic redistribution of mineral elements has been observed, thus uncovering a possible role for mineral nutrients in the response against pathogens. On the contrary, the resistant cultivars did not present substantial rearrangement of mineral elements at leaf level, except for manganese (Mn) and iron (Fe). This might demonstrate that, resistant cultivars, albeit expressing the resistance gene, still exploit a pathogen response mechanism based on the local increase in the concentration of microelements, which are involved in the synthesis of secondary metabolites and reactive oxygen species. Moreover, these data also highlight the link between the mineral nutrition and plants’ response to pathogens, further stressing that appropriate fertilization strategies can be fundamental for the expression of response mechanisms against pathogens.
Stefano Cesco; Anna Tolotti; Stefano Nadalini; Stefano Rizzi; Fabio Valentinuzzi; Tanja Mimmo; Carlo Porfido; Ignazio Allegretta; Oscar Giovannini; Michele Perazzolli; Guido Cipriani; Roberto Terzano; Ilaria Pertot; Youry Pii. Plasmopara viticola infection affects mineral elements allocation and distribution in Vitis vinifera leaves. Scientific Reports 2020, 10, 1 -18.
AMA StyleStefano Cesco, Anna Tolotti, Stefano Nadalini, Stefano Rizzi, Fabio Valentinuzzi, Tanja Mimmo, Carlo Porfido, Ignazio Allegretta, Oscar Giovannini, Michele Perazzolli, Guido Cipriani, Roberto Terzano, Ilaria Pertot, Youry Pii. Plasmopara viticola infection affects mineral elements allocation and distribution in Vitis vinifera leaves. Scientific Reports. 2020; 10 (1):1-18.
Chicago/Turabian StyleStefano Cesco; Anna Tolotti; Stefano Nadalini; Stefano Rizzi; Fabio Valentinuzzi; Tanja Mimmo; Carlo Porfido; Ignazio Allegretta; Oscar Giovannini; Michele Perazzolli; Guido Cipriani; Roberto Terzano; Ilaria Pertot; Youry Pii. 2020. "Plasmopara viticola infection affects mineral elements allocation and distribution in Vitis vinifera leaves." Scientific Reports 10, no. 1: 1-18.
Due to the deliberate use of cupric fungicides in the last century for crop-defence programs, copper (Cu) has considerably accumulated in the soil. The concentrations of Cu often exceed the safety limits of risk assessment for Cu in soil and this may cause toxicity in plants. Copper toxicity induces nutritional imbalances in plants and constraints to plants growth. These aspects might be of paramount importance in the case of phosphorus (P), which is an essential plant macronutrient. In this work, hydroponically grown cucumber plants were used to investigate the influence of the exposure to different Cu concentrations (0.2, 5, 25 and 50 μM) on i) the phenotypic traits of plants, particularly at root level, ii) the nutrient content in both roots and shoots, and iii) the P uptake mechanisms, considering both the biochemical and molecular aspects. At high Cu concentrations (i.e. above 25 μM), the shoot and root growth resulted stunted and the P influx rate diminished. Furthermore, two P transporter genes (i.e. CsPT1.4 and CsPT1.9) were upregulated at the highest Cu concentration, albeit with different induction kinetics. Overall, these results confirm that high Cu concentrations can limit the root acquisition of P, most likely via a direct action on the uptake mechanisms (e.g. transporters). However, the alteration of root plasma membrane permeability induced by Cu toxicity might also play a pivotal role in the observed phenomenon.
Sebastian B. Feil; Youry Pii; Fabio Valentinuzzi; Raphael Tiziani; Tanja Mimmo; Stefano Cesco. Copper toxicity affects phosphorus uptake mechanisms at molecular and physiological levels in Cucumis sativus plants. Plant Physiology and Biochemistry 2020, 157, 138 -147.
AMA StyleSebastian B. Feil, Youry Pii, Fabio Valentinuzzi, Raphael Tiziani, Tanja Mimmo, Stefano Cesco. Copper toxicity affects phosphorus uptake mechanisms at molecular and physiological levels in Cucumis sativus plants. Plant Physiology and Biochemistry. 2020; 157 ():138-147.
Chicago/Turabian StyleSebastian B. Feil; Youry Pii; Fabio Valentinuzzi; Raphael Tiziani; Tanja Mimmo; Stefano Cesco. 2020. "Copper toxicity affects phosphorus uptake mechanisms at molecular and physiological levels in Cucumis sativus plants." Plant Physiology and Biochemistry 157, no. : 138-147.
Inoculation with plant growth-promoting bacteria (PGPB) represents an efficient method in sustainable agriculture to improve nutrients availability and crop production in diverse environmental conditions. In the present work, untargeted metabolomics and community-level physiological profiles (CLPP) approaches were employed to investigate the shaping of tomato rhizosphere functioning and potential metabolic activity imposed by rhizosphere-associated microbiome, following inoculation with two different PGPB (Enterobacter sp. 15S and Pseudomonas sp. 16S). Significant increases in root and shoot dry biomass were observed in both inoculated treatments, when compared to uninoculated plants (p < 0.05). The untargeted metabolomics allowed discriminating the metabolic profiles of tomato rhizosphere, with distinct modulations imposed by either Enterobacter 15S or Pseudomonas 16S. Flavonoids and other phenolics were among the most frequently identified differential metabolites in the rhizosphere from both Enterobacter 15S and Pseudomonas 16S inoculated plants. Nevertheless, other metabolites like phytohormones and amino acids were also decisive to this specific modulation. The metabolic activity profile rhizosphere-associated microbiome of tomato plants unveiled by the CLPP analysis was congruent with the metabolomic data and reinforced the influence exerted by the bacterial inoculation on modulating the rhizosphere microbiome functioning. In particular, the microbiome associated with control and 16S-treated plants showed a higher functional diversity than those treated with Enterobacter 15S. Carbohydrates, carboxylic acids, amino acids, and polymers were the main classes of substrates which contributed to such differences.
Mónica Yorlady Alzate Zuluaga; Karina Maria Lima Milani; Begoña Miras-Moreno; Luigi Lucini; Fabio Valentinuzzi; Tanja Mimmo; Youry Pii; Stefano Cesco; Elisete Pains Rodrigues; André Luiz Martinez de Oliveira. Inoculation with plant growth-promoting bacteria alters the rhizosphere functioning of tomato plants. Applied Soil Ecology 2020, 158, 103784 .
AMA StyleMónica Yorlady Alzate Zuluaga, Karina Maria Lima Milani, Begoña Miras-Moreno, Luigi Lucini, Fabio Valentinuzzi, Tanja Mimmo, Youry Pii, Stefano Cesco, Elisete Pains Rodrigues, André Luiz Martinez de Oliveira. Inoculation with plant growth-promoting bacteria alters the rhizosphere functioning of tomato plants. Applied Soil Ecology. 2020; 158 ():103784.
Chicago/Turabian StyleMónica Yorlady Alzate Zuluaga; Karina Maria Lima Milani; Begoña Miras-Moreno; Luigi Lucini; Fabio Valentinuzzi; Tanja Mimmo; Youry Pii; Stefano Cesco; Elisete Pains Rodrigues; André Luiz Martinez de Oliveira. 2020. "Inoculation with plant growth-promoting bacteria alters the rhizosphere functioning of tomato plants." Applied Soil Ecology 158, no. : 103784.
The reliable quantification of root exudation and nutrient uptake is a very challenging task, especially when considering single root segments. Most methods used necessitate root handling e.g. root dissecting/cutting. However, there is a knowledge gap on how much these techniques affect root physiology. Thus, this study aimed at assessing the effect of different root handling techniques on the phosphate (Pi) uptake and carboxylate exudation of white lupin roots. White lupin plants were grown hydroponically in a full and Pi-deficient nutrient solution for 60 days. Phosphate uptake and carboxylate exudation of cluster and non-cluster roots were measured using custom made cells 1, 4, and 8 h after the onset of light. Three different experimental set-ups were used: i) without cutting the root apparatus from the shoots, nor dissecting the root into smaller root sections — named intact plant (IP); ii) separating the roots from the shoots, without dissecting the root into smaller sections — named intact root (IR); iii) separating the roots form the shoots and dissecting the roots in different sections—named dissected roots (DR). The sampling at 8 h led to the most significant alterations of the root Pi uptake induced by the sampling method. Generally, roots were mainly affected by the DR sampling method, indicating that results of studies in which roots are cut/dissected should be interpreted carefully. Additionally, the study revealed that the root tip showed a very high Pi uptake rate, suggesting that the tip could act as a Pi sensor. Citrate, malate and lactate could be detected in juvenile, mature and senescent cluster root exudation. We observed a significant effect of the handling method on carboxylate exudation only at sampling hours 1 and 8, although no clear and distinctive trend could be observed. Results here presented reveal that the root handling as well as the sampling time point can greatly influence root physiology and therefore should not be neglected when interpreting rhizosphere dynamics.
Raphael Tiziani; Tanja Mimmo; Fabio Valentinuzzi; Youry Pii; Silvia Celletti; Stefano Cesco. Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots. Frontiers in Plant Science 2020, 11, 584568 .
AMA StyleRaphael Tiziani, Tanja Mimmo, Fabio Valentinuzzi, Youry Pii, Silvia Celletti, Stefano Cesco. Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots. Frontiers in Plant Science. 2020; 11 ():584568.
Chicago/Turabian StyleRaphael Tiziani; Tanja Mimmo; Fabio Valentinuzzi; Youry Pii; Silvia Celletti; Stefano Cesco. 2020. "Root Handling Affects Carboxylates Exudation and Phosphate Uptake of White Lupin Roots." Frontiers in Plant Science 11, no. : 584568.
Plant roots are able to exude vast amounts of metabolites into the rhizosphere in response to phosphorus (P) deficiency. Causing noteworthy costs in terms of energy and carbon (C) for the plants. Therefore, it is suggested that exudates reacquisition by roots could represent an energy saving strategy of plants. This study aimed at investigating the effect of P deficiency on the ability of hydroponically grown tomato plants to re-acquire specific compounds generally present in root exudates by using 13C-labelled molecules. Results showed that P deficient tomato plants were able to take up citrate (+ 37%) and malate (+ 37%), particularly when compared to controls. While glycine (+ 42%) and fructose (+ 49%) uptake was enhanced in P shortage, glucose acquisition was not affected by the nutritional status. Unexpectedly, results also showed that P deficiency leads to a 13C enrichment in both tomato roots and shoots over time (shoots—+ 2.66‰, roots—+ 2.64‰, compared to control plants), probably due to stomata closure triggered by P deficiency. These findings highlight that tomato plants are able to take up a wide range of metabolites belonging to root exudates, thus maximizing C trade off. This trait is particularly evident when plants grew in P deficiency.
Raphael Tiziani; Youry Pii; Silvia Celletti; Stefano Cesco; Tanja Mimmo. Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants. Scientific Reports 2020, 10, 1 -12.
AMA StyleRaphael Tiziani, Youry Pii, Silvia Celletti, Stefano Cesco, Tanja Mimmo. Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants. Scientific Reports. 2020; 10 (1):1-12.
Chicago/Turabian StyleRaphael Tiziani; Youry Pii; Silvia Celletti; Stefano Cesco; Tanja Mimmo. 2020. "Phosphorus deficiency changes carbon isotope fractionation and triggers exudate reacquisition in tomato plants." Scientific Reports 10, no. 1: 1-12.
The aim of this work was to study the biological catalysts and possible substrate conversion routes in mesophilic dark fermentation reactors aimed at producing H2 from olive mill wastewater. Bacillus and Clostridium were the most abundant phylotypes during the rapid stage of H2 production. Chemical analyses combined with predictive functional profiling of the bacterial communities indicated that the lactate fermentation was the main H2-producing route. In fact, during the fermentation process, lactate and acetate were consumed, while H2 and butyrate were being produced. The fermentation process was rich in genes that encode enzymes for lactate generation from pyruvate. Lactate conversion to butyrate through the generation of pyruvate produced H2 through the recycling of electron carriers via the pyruvate ferredoxin oxydoreductase pathway. Overall, these findings showed the synergy among lactate-, acetate- and H2-producing bacteria, which complex interactions determine the H2 production routes in the bioreactors.
Gianmarco Mugnai; Luigimaria Borruso; Tanja Mimmo; Stefano Cesco; Vincenzo Luongo; Luigi Frunzo; Massimiliano Fabbricino; Francesco Pirozzi; Francesca Cappitelli; Federica Villa. Dynamics of bacterial communities and substrate conversion during olive-mill waste dark fermentation: Prediction of the metabolic routes for hydrogen production. Bioresource Technology 2020, 319, 124157 .
AMA StyleGianmarco Mugnai, Luigimaria Borruso, Tanja Mimmo, Stefano Cesco, Vincenzo Luongo, Luigi Frunzo, Massimiliano Fabbricino, Francesco Pirozzi, Francesca Cappitelli, Federica Villa. Dynamics of bacterial communities and substrate conversion during olive-mill waste dark fermentation: Prediction of the metabolic routes for hydrogen production. Bioresource Technology. 2020; 319 ():124157.
Chicago/Turabian StyleGianmarco Mugnai; Luigimaria Borruso; Tanja Mimmo; Stefano Cesco; Vincenzo Luongo; Luigi Frunzo; Massimiliano Fabbricino; Francesco Pirozzi; Francesca Cappitelli; Federica Villa. 2020. "Dynamics of bacterial communities and substrate conversion during olive-mill waste dark fermentation: Prediction of the metabolic routes for hydrogen production." Bioresource Technology 319, no. : 124157.