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Two rice accessions, Capataz and Beirao, contrasting for cadmium (Cd) tolerance and root retention, were exposed to a broad range of Cd concentrations (0.01, 0.1, and 1 μM) and analyzed for their potential capacity to chelate, compartmentalize, and translocate Cd to gain information about the relative contribution of these processes in determining the different pathways of Cd distribution along the plants. In Capataz, Cd root retention increased with the external Cd concentration, while in Beirao it resulted independent of Cd availability and significantly higher than in Capataz at the lowest Cd concentrations analyzed. Analysis of thiol accumulation in the roots revealed that the different amounts of these compounds in Capataz and Beirao, as well as the expression levels of genes involved in phytochelatin biosynthesis and direct Cd sequestration into the vacuoles of the root cells, were not related to the capacity of the accessions to trap the metal into the roots. Interestingly, the relative transcript abundance of OsHMA2, a gene controlling root-to-shoot Cd/Zn translocation, was not influenced by Cd exposure in Capataz and progressively increased in Beirao with the external Cd concentration, suggesting that activity of the OsHMA2 transporter may differentially limit root-to-shoot Cd/Zn translocation in Capataz and Beirao.
Moez Maghrebi; Elena Baldoni; Giorgio Lucchini; Gianpiero Vigani; Giampiero Valè; Gian Sacchi; Fabio Nocito. Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2. Plants 2021, 10, 806 .
AMA StyleMoez Maghrebi, Elena Baldoni, Giorgio Lucchini, Gianpiero Vigani, Giampiero Valè, Gian Sacchi, Fabio Nocito. Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2. Plants. 2021; 10 (4):806.
Chicago/Turabian StyleMoez Maghrebi; Elena Baldoni; Giorgio Lucchini; Gianpiero Vigani; Giampiero Valè; Gian Sacchi; Fabio Nocito. 2021. "Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2." Plants 10, no. 4: 806.
Formate dehydrogenase (FDH) catalyzes the conversion of formate (HCOO-) into carbon dioxide (CO2), coupled with the reduction of NAD+ to NADH. FDH involvement in the response against abiotic stress is well established whereas FDH role against pathogen attack is less known. Therefore, the in silico correlation analysis of the FDH transcript with a biotic stress dataset was performed, which highlighted correlation with genes involved in the response against pathogen attack. Next, a reduction in the expression of an FDH reporter construct in the hydathodes of Arabidopsis thaliana leaves infected with Xanthomonas campestris pv campestris (Xcc) was observed. We also observed an increased proliferation of Xcc in the hydathodes of an atfdh1-5 mutant during the early stages of infection, which further supports FDH involvement in an early defense response activated in the hydathodes upon Xcc infection, possibly through modulation of formate levels.
Francesca Marzorati; Gianpiero Vigani; Piero Morandini; Irene Murgia. Formate dehydrogenase contributes to the early Arabidopsis thaliana responses against Xanthomonas campestris pv campestris infection. Physiological and Molecular Plant Pathology 2021, 114, 101633 .
AMA StyleFrancesca Marzorati, Gianpiero Vigani, Piero Morandini, Irene Murgia. Formate dehydrogenase contributes to the early Arabidopsis thaliana responses against Xanthomonas campestris pv campestris infection. Physiological and Molecular Plant Pathology. 2021; 114 ():101633.
Chicago/Turabian StyleFrancesca Marzorati; Gianpiero Vigani; Piero Morandini; Irene Murgia. 2021. "Formate dehydrogenase contributes to the early Arabidopsis thaliana responses against Xanthomonas campestris pv campestris infection." Physiological and Molecular Plant Pathology 114, no. : 101633.
In recent decades, agriculture has faced the fundamental challenge of needing to increase food production and quality in order to meet the requirements of a growing global population. Similarly, viticulture has also been undergoing change. Several countries are reducing their vineyard areas, and several others are increasing them. In addition, viticulture is moving towards higher altitudes and latitudes due to climate change. Furthermore, global warming is also exacerbating the incidence of fungal diseases in vineyards, forcing farmers to apply agrochemicals to preserve production yields and quality. The repeated application of copper (Cu)-based fungicides in conventional and organic farming has caused a stepwise accumulation of Cu in vineyard soils, posing environmental and toxicological threats. High Cu concentrations in soils can have multiple impacts on agricultural systems. In fact, it can (i) alter the chemical-physical properties of soils, thus compromising their fertility; (ii) induce toxicity phenomena in plants, producing detrimental effects on growth and productivity; and (iii) affect the microbial biodiversity of soils, thereby influencing some microbial-driven soil processes. However, several indirect (e.g., management of rhizosphere processes through intercropping and/or fertilization strategies) and direct (e.g., exploitation of vine resistant genotypes) strategies have been proposed to restrain Cu accumulation in soils. Furthermore, the application of precision and smart viticulture paradigms and their related technologies could allow a timely, localized and balanced distribution of agrochemicals to achieve the required goals. The present review highlights the necessity of applying multidisciplinary approaches to meet the requisites of sustainability demanded of modern viticulture.
Stefano Cesco; Youry Pii; Luigimaria Borruso; Guido Orzes; Paolo Lugli; Fabrizio Mazzetto; Giulio Genova; Marco Signorini; Gustavo Brunetto; Roberto Terzano; Gianpiero Vigani; Tanja Mimmo. A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity. Applied Sciences 2021, 11, 907 .
AMA StyleStefano Cesco, Youry Pii, Luigimaria Borruso, Guido Orzes, Paolo Lugli, Fabrizio Mazzetto, Giulio Genova, Marco Signorini, Gustavo Brunetto, Roberto Terzano, Gianpiero Vigani, Tanja Mimmo. A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity. Applied Sciences. 2021; 11 (3):907.
Chicago/Turabian StyleStefano Cesco; Youry Pii; Luigimaria Borruso; Guido Orzes; Paolo Lugli; Fabrizio Mazzetto; Giulio Genova; Marco Signorini; Gustavo Brunetto; Roberto Terzano; Gianpiero Vigani; Tanja Mimmo. 2021. "A Smart and Sustainable Future for Viticulture Is Rooted in Soil: How to Face Cu Toxicity." Applied Sciences 11, no. 3: 907.
The Geomagnetic field (GMF) is a typical component of our planet. Plant perception of the GMF implies that any magnetic field (MF) variation would induce possible metabolic changes. In this work was we assessed the role of the GMF on Arabidopsis thaliana Col0 mineral nutrition and lipid metabolism during plant development. We reduced the local GMF (about 40 μT) to Near Null Magnetic Field (NNMF, about 30 nT) to evaluate the effects of GMF on Arabidopsis in a time-course (from rosette to seed-set) experiment by studying the lipid content (fatty acids, FA; and surface alkanes, SA) and mineral nutrients. The expression of selected genes involved in lipid metabolism was assessed by Real-Time PCR (qPCR). A progressive increase of SA with carbon numbers between 21 and 28 was found in plants exposed to NNMF from bolting to flowering developmental stages, whereas the content of some FA significantly (p < 0.05) increased in rosette, bolting and seed-set developmental stages. Variations in SA composition were correlated to the differential expression of several Arabidopsis 3-ketoacyl-CoAsynthase (KCS) genes, including KCS1, KCS5, KCS6, KCS8, and KCS12, a lipid transfer protein (LTPG1) and a lipase (LIP1). Ionomic analysis showed a significant variation in some micronutrients (Fe, Co, Mn and Ni) and macronutrients (Mg, K and Ca) during plant development of plants exposed to NNMF. The results of this work show that A. thaliana responds to variations of the GMF which are perceived as is typical of abiotic stress responses.
Monirul Islam; Gianpiero Vigani; Massimo E. Maffei. The Geomagnetic Field (GMF) Modulates Nutrient Status and Lipid Metabolism during Arabidopsis thaliana Plant Development. Plants 2020, 9, 1729 .
AMA StyleMonirul Islam, Gianpiero Vigani, Massimo E. Maffei. The Geomagnetic Field (GMF) Modulates Nutrient Status and Lipid Metabolism during Arabidopsis thaliana Plant Development. Plants. 2020; 9 (12):1729.
Chicago/Turabian StyleMonirul Islam; Gianpiero Vigani; Massimo E. Maffei. 2020. "The Geomagnetic Field (GMF) Modulates Nutrient Status and Lipid Metabolism during Arabidopsis thaliana Plant Development." Plants 9, no. 12: 1729.
Cristiana Ariotti; Elena Giuliano; Paolina Garbeva; Gianpiero Vigani. The Fascinating World of Belowground Communication. Frontiers for Young Minds 2020, 8, 1 .
AMA StyleCristiana Ariotti, Elena Giuliano, Paolina Garbeva, Gianpiero Vigani. The Fascinating World of Belowground Communication. Frontiers for Young Minds. 2020; 8 ():1.
Chicago/Turabian StyleCristiana Ariotti; Elena Giuliano; Paolina Garbeva; Gianpiero Vigani. 2020. "The Fascinating World of Belowground Communication." Frontiers for Young Minds 8, no. : 1.
Parietaria judaica grows in highly calcareous environments, overcoming the low bioavailability of Fe caused by elevated pH. The aim of this work was to investigate the temporal dynamics of root exudation of P. judaica under Fe deficiency conditions. As high concentrations of bicarbonate and Ca2+ in calcareous soils interfere with the general plant mineral nutrition, two different alkaline growing conditions were applied to distinguish the effects due to the high pH from the responses induced by the presence of high calcium carbonate concentrations. Growth parameters and physiological responses were analyzed during a 7 day time course—shoot and root biomass, chlorophyll and flavonoid contents in leaves, root accumulation, and exudation of organic acids and phenolics were determined. Different responses were found in plants grown in the presence of bicarbonate and in the presence of an organic pH buffer, revealing a time- and condition-dependent response of P. judaica and suggesting a stronger stress in the buffer treatment. The high tolerance to alkaline conditions may be related to an earlier and greater exudation rate of phenolics, as well as to the synergistic effect of phenolics and carboxylic acids in root exudates in the late response. The identification of the main functional traits involved in tolerance to low Fe availability in a wild species could offer crucial inputs for breeding programs for application to crop species.
Liliana Tato; Monirul Islam; Tanja Mimmo; Graziano Zocchi; Gianpiero Vigani. Temporal Responses to Direct and Induced Iron Deficiency in Parietaria judaica. Agronomy 2020, 10, 1037 .
AMA StyleLiliana Tato, Monirul Islam, Tanja Mimmo, Graziano Zocchi, Gianpiero Vigani. Temporal Responses to Direct and Induced Iron Deficiency in Parietaria judaica. Agronomy. 2020; 10 (7):1037.
Chicago/Turabian StyleLiliana Tato; Monirul Islam; Tanja Mimmo; Graziano Zocchi; Gianpiero Vigani. 2020. "Temporal Responses to Direct and Induced Iron Deficiency in Parietaria judaica." Agronomy 10, no. 7: 1037.
WHIRLY2 is a single‐stranded DNA binding protein associated with mitochondrial nucleoids. In the why 2‐1 mutant of Arabidopsis thaliana , a major proportion of leaf mitochondria has an aberrant structure characterized by disorganized nucleoids, reduced abundance of cristae, and a low matrix density despite the fact that the macroscopic phenotype during vegetative growth is not different from wild type. These features coincide with an impairment of the functionality and dynamics of mitochondria that have been characterized in detail in wild‐type and why 2‐1 mutant cell cultures. In contrast to the development of the vegetative parts, seed germination is compromised in the why 2‐1 mutant. In line with that, the expression level of why 2 in seeds of wild‐type plants is higher than that of why 3 , whereas in adult plant no difference is found. Intriguingly, in early stages of shoots development of the why 2‐1 mutant, although not in seeds, the expression level of why 3 is enhanced. These results suggest that WHIRLY3 is a potential candidate to compensate for the lack of WHIRLY2 in the why 2‐1 mutant. Such compensation is possible only if the two proteins are localized in the same organelle. Indeed, in organello protein transport experiments using intact mitochondria and chloroplasts revealed that WHIRLY3 can be dually targeted into both, chloroplasts and mitochondria. Together, these data indicate that the alterations of mitochondria nucleoids are tightly linked to alterations of mitochondria morphology and functionality. This is even more evident in those phases of plant life when mitochondrial activity is particularly high, such as seed germination. Moreover, our results indicate that the differential expression of why 2 and why 3 predetermines the functional replacement of WHIRLY2 by WHIRLY3, which is restricted though to the vegetative parts of the plant.
Serena Golin; Yuri L. Negroni; Bationa Bennewitz; Ralf B. Klösgen; Maria Mulisch; Nicoletta La Rocca; Francesca Cantele; Gianpiero Vigani; Fiorella Lo Schiavo; Karin Krupinska; Michela Zottini. WHIRLY2 plays a key role in mitochondria morphology, dynamics, and functionality in Arabidopsis thaliana. Plant Direct 2020, 4, 1 .
AMA StyleSerena Golin, Yuri L. Negroni, Bationa Bennewitz, Ralf B. Klösgen, Maria Mulisch, Nicoletta La Rocca, Francesca Cantele, Gianpiero Vigani, Fiorella Lo Schiavo, Karin Krupinska, Michela Zottini. WHIRLY2 plays a key role in mitochondria morphology, dynamics, and functionality in Arabidopsis thaliana. Plant Direct. 2020; 4 (5):1.
Chicago/Turabian StyleSerena Golin; Yuri L. Negroni; Bationa Bennewitz; Ralf B. Klösgen; Maria Mulisch; Nicoletta La Rocca; Francesca Cantele; Gianpiero Vigani; Fiorella Lo Schiavo; Karin Krupinska; Michela Zottini. 2020. "WHIRLY2 plays a key role in mitochondria morphology, dynamics, and functionality in Arabidopsis thaliana." Plant Direct 4, no. 5: 1.
Low Fe availability affects plant production mainly by impairing the photosynthetic pathway, since Fe plays an essential role in chlorophyll synthesis as well as in the photosynthetic electron transport chain. Under these conditions, plant cells require the activation of protective mechanisms to prevent photo-inhibition. Among these mechanisms, photorespiration (PR) has been relatively little investigated in Fe-deficient plants. The aim of this work was to investigate the effect of Fe deficiency on photorespiration by performing in vivo analysis in leaves as well as biochemical characterization of some PR-related enzyme activities in a peroxisome-purified fraction from cucumber leaves. Modelling of light response curves at both 21 and 2% pO2 revealed a slowing down of PR under Fe deficiency. The activity of some PR-involving enzymes as well as the contents of glycine and serine were affected under Fe deficiency. Furthermore, nitrate reductase, the glutamine synthetase-glutamate synthase (GS-GOGAT) cycle and hydroxypyruvate dehydrogenase isoform activities were differentially altered under Fe deficiency. The dataset indicates that, in Fe-deficient cucumber leaves, the modulation of PR involves the induction of some PR-related pathways, such as the photorespiratory N recycling and cytosolic photorespiratory bypass processes.
Fabio M. Casiraghi; Marco Landi; Silvia Donnini; Andrea Borlotti; Graziano Zocchi; Lucia Guidi; Gianpiero Vigani. Modulation of photorespiration and nitrogen recycling in Fe-deficient cucumber leaves. Plant Physiology and Biochemistry 2020, 154, 142 -150.
AMA StyleFabio M. Casiraghi, Marco Landi, Silvia Donnini, Andrea Borlotti, Graziano Zocchi, Lucia Guidi, Gianpiero Vigani. Modulation of photorespiration and nitrogen recycling in Fe-deficient cucumber leaves. Plant Physiology and Biochemistry. 2020; 154 ():142-150.
Chicago/Turabian StyleFabio M. Casiraghi; Marco Landi; Silvia Donnini; Andrea Borlotti; Graziano Zocchi; Lucia Guidi; Gianpiero Vigani. 2020. "Modulation of photorespiration and nitrogen recycling in Fe-deficient cucumber leaves." Plant Physiology and Biochemistry 154, no. : 142-150.
The Earth’s magnetic field, defined as the geomagnetic field (GMF), is an unavoidable environmental factor for all living organisms. Variation in the GMF intensity was found to affect the content of some nutrients and their associated channels and transporters in Arabidopsis thaliana. In this work, we observed that reduction of the GMF to near null magnetic field (NNMF) affects the accumulation of metals in plant tissues, mainly iron (Fe) and zinc (Zn) content, while the content of others metals such as copper (Cu) and manganese (Mn) is not affected. Accordingly, Fe uptake genes were induced in the roots of NNMF-exposed plants and the root Fe reductase activity was affected by transferring GMF-exposed plant to NNMF condition. Under Fe deficiency, NNMF-exposed plants displayed a limitation in the activation of Fe-deficiency induced genes. Such an effect was associated with the strong accumulation of Zn and Cu observed under NNMF conditions. Overall, our results provide evidence on the important role of the GMF on the iron uptake efficiency of plants.
Monirul Islam; Massimo Maffei; Gianpiero Vigani. The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana. Frontiers in Plant Science 2020, 11, 325 .
AMA StyleMonirul Islam, Massimo Maffei, Gianpiero Vigani. The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana. Frontiers in Plant Science. 2020; 11 ():325.
Chicago/Turabian StyleMonirul Islam; Massimo Maffei; Gianpiero Vigani. 2020. "The Geomagnetic Field Is a Contributing Factor for an Efficient Iron Uptake in Arabidopsis thaliana." Frontiers in Plant Science 11, no. : 325.
Irene Murgia; Gianpiero Vigani; Dario Di Silvestre; Pierluigi Mauri; Rossana Rossi; Andrea Bergamaschi; Miriam Frisella; Piero Morandini. Formate dehydrogenase takes part in molybdenum and iron homeostasis and affects dark-induced senescence in plants. Journal of Plant Interactions 2020, 15, 386 -397.
AMA StyleIrene Murgia, Gianpiero Vigani, Dario Di Silvestre, Pierluigi Mauri, Rossana Rossi, Andrea Bergamaschi, Miriam Frisella, Piero Morandini. Formate dehydrogenase takes part in molybdenum and iron homeostasis and affects dark-induced senescence in plants. Journal of Plant Interactions. 2020; 15 (1):386-397.
Chicago/Turabian StyleIrene Murgia; Gianpiero Vigani; Dario Di Silvestre; Pierluigi Mauri; Rossana Rossi; Andrea Bergamaschi; Miriam Frisella; Piero Morandini. 2020. "Formate dehydrogenase takes part in molybdenum and iron homeostasis and affects dark-induced senescence in plants." Journal of Plant Interactions 15, no. 1: 386-397.
Volatile organic compounds displayed biological activities on a wide range of organisms, including plants and microbes. Investigating their role in the plant-microbe interaction processes occurring in the soil is challenging. By simulating belowground communication conditions between plant and microbes, in this study, we aimed to investigate the effects of the volatiles emitted by Serratia plymuthica and Fusarium culmorum on the nutrient status of maize plants. Plants were grown in potting soil and exposed to volatiles emitted by microbes inoculated in Petri dishes at the bottom of a jar. Nutrients content of plant tissues as well as soil volatiles were analyzed by ICP-MS and GC-MS, respectively. Our results showed that volatiles emitted belowground by Serratia plymuthica and Fusarium culmorum, in monoculture or interaction, differentially impacted on the content of some nutrient in plants, indicating that microbial volatiles-emitted belowground can affect the nutritional status of plants from a distance.
Lara Martín-Sánchez; Cristiana Ariotti; Paolina Garbeva; Gianpiero Vigani. Investigating the effect of belowground microbial volatiles on plant nutrient status: perspective and limitations. Journal of Plant Interactions 2020, 15, 188 -195.
AMA StyleLara Martín-Sánchez, Cristiana Ariotti, Paolina Garbeva, Gianpiero Vigani. Investigating the effect of belowground microbial volatiles on plant nutrient status: perspective and limitations. Journal of Plant Interactions. 2020; 15 (1):188-195.
Chicago/Turabian StyleLara Martín-Sánchez; Cristiana Ariotti; Paolina Garbeva; Gianpiero Vigani. 2020. "Investigating the effect of belowground microbial volatiles on plant nutrient status: perspective and limitations." Journal of Plant Interactions 15, no. 1: 188-195.
Increasing crop yields by using eco‐friendly practices is of high priority to tackle problems regarding food security and malnutrition worldwide. A sustainable crop production requires a limited use of fertilizer as well as the employment of plant varieties with improved ability to acquire nutrients form soil. To reach these goals, the scientific community aims to understand plant nutrients homeostasis by deciphering the nutrient sensing and signaling mechanisms of plants. Several lines of evidence about the involvement of Ca2+ as the signal of an impaired nutrient availability have been reported. Ca2+ signaling is a tightly regulated process which requires specific protein toolkits to perceive external stimuli and to induce the specific responses in the plant needed to survive. Here we summarize both older and recent findings concerning the involvement of Ca2+ signaling in the homeostasis of nutrients. In this review we present new emerging technologies, based on the use of genetically encoded Ca2+ sensors and advanced microscopy, that offer the chance to perform in planta analyses of Ca2+ dynamics at cellular resolution. The harnessing of these technologies with different genetic backgrounds and subjected to different nutritional stresses will provide important insights to the still little‐known mechanisms of nutrient sensing in plants.
Gianpiero Vigani; Alex Costa. Harnessing the new emerging imaging technologies to uncover the role of Ca2+signalling in plant nutrient homeostasis. Plant, Cell & Environment 2019, 42, 2885 -2901.
AMA StyleGianpiero Vigani, Alex Costa. Harnessing the new emerging imaging technologies to uncover the role of Ca2+signalling in plant nutrient homeostasis. Plant, Cell & Environment. 2019; 42 (10):2885-2901.
Chicago/Turabian StyleGianpiero Vigani; Alex Costa. 2019. "Harnessing the new emerging imaging technologies to uncover the role of Ca2+signalling in plant nutrient homeostasis." Plant, Cell & Environment 42, no. 10: 2885-2901.
Iron (Fe) is a cofactor required for a variety of essential redox reactions in plant metabolism. Thus, plants have developed a complex network of interacting pathways to withstand Fe deficiency, including metabolic reprogramming. This opinion aims at revisiting such reprogramming by focusing on: (i) the functional relationships of Fe-requiring enzymes (FeREs) with respect to oxygen; and (ii) the progression of FeREs engagement, occurring under Fe deficiency stress. In particular, we considered such progression of FeREs engagement as strain responses of increasing severity during the stress phases of alarm, resistance, and exhaustion. This approach can contribute to reconcile the variety of experimental results obtained so far from different plant species and/or different Fe supplies.
Gianpiero Vigani; Irene Murgia. Iron-Requiring Enzymes in the Spotlight of Oxygen. Trends in Plant Science 2018, 23, 874 -882.
AMA StyleGianpiero Vigani, Irene Murgia. Iron-Requiring Enzymes in the Spotlight of Oxygen. Trends in Plant Science. 2018; 23 (10):874-882.
Chicago/Turabian StyleGianpiero Vigani; Irene Murgia. 2018. "Iron-Requiring Enzymes in the Spotlight of Oxygen." Trends in Plant Science 23, no. 10: 874-882.
Multilevel interactions among nutrients occur in the soil-plant system. Among them, Fe and Zn homeostasis in plants are of great relevance because of their importance for plant and human nutrition. However, the mechanisms underlying the interplay between Fe and Zn in plants are still poorly understood. In order to elucidate how Zn interacts with Fe homeostasis, it is crucial to assess Zn distribution either in the plant tissues or within the cells. In this study, we investigated the subcellular Zn distribution in Fe-deficient leaf cells of cucumber plants by using two different approaches: cellular fractionation coupled with inductively coupled plasma mass spectrometry (ICP/MS) and nanoscopic synchrotron X-ray fluorescence imaging. Fe-deficient leaves showed a strong accumulation of Zn as well as a strong alteration of the organelles’ ultrastructure at the cellular level. The cellular fractionation-ICP/MS approach revealed that Zn accumulates in both chloroplasts and mitochondria of Fe deficient leaves. Nano-XRF imaging revealed Zn accumulation in chloroplast and mitochondrial compartments, with a higher concentration in chloroplasts. Such results show that (i) both approaches are suitable to investigate Zn distribution at the subcellular level and (ii) cellular Fe and Zn interactions take place mainly in the organelles, especially in the chloroplasts.
Gianpiero Vigani; Sylvain Bohic; Franco Faoro; Bart Vekemans; Lazlo Vincze; Roberto Terzano. Cellular Fractionation and Nanoscopic X-Ray Fluorescence Imaging Analyses Reveal Changes of Zinc Distribution in Leaf Cells of Iron-Deficient Plants. Frontiers in Plant Science 2018, 9, 1 .
AMA StyleGianpiero Vigani, Sylvain Bohic, Franco Faoro, Bart Vekemans, Lazlo Vincze, Roberto Terzano. Cellular Fractionation and Nanoscopic X-Ray Fluorescence Imaging Analyses Reveal Changes of Zinc Distribution in Leaf Cells of Iron-Deficient Plants. Frontiers in Plant Science. 2018; 9 ():1.
Chicago/Turabian StyleGianpiero Vigani; Sylvain Bohic; Franco Faoro; Bart Vekemans; Lazlo Vincze; Roberto Terzano. 2018. "Cellular Fractionation and Nanoscopic X-Ray Fluorescence Imaging Analyses Reveal Changes of Zinc Distribution in Leaf Cells of Iron-Deficient Plants." Frontiers in Plant Science 9, no. : 1.
Within the last years, extensive information has been accumulated on the reciprocal influence between S and Fe nutrition at both physiological and molecular level in several plant species, but the mechanisms regulating S and Fe sensing and signaling are not fully understood. Fe and S interact for the building of Fe-S clusters, and mitochondria is one of the cellular compartments where Fe-S cluster assembly takes place. Therefore, it would be expected that mitochondria might play a central role in the regulation of Fe and S interaction. The Fe deficiency-induced alteration in the synthesis of mitochondria-derived carboxylic acids, such as citric acid, and the evidence that such molecules have already been identified as important players of metabolite signaling in several organisms, further support this hypothesis. Tomato plants were grown under single or combined Fe and S deficiency with the aim of verifying whether mitochondria activities played a role in Fe/S interaction. Both Fe and S deficiencies determined similar alteration of respiratory chain activity: a general decrease of Fe-S containing complexes as well as an increase of alternative NAD(P)H activities was observed in both Fe and S deficient-plants. However, the content of Krebs cycle-related organic acids in roots was substantially different in response to treatments, being the accumulation of citric acid always increased, while the others (i.e. succinic, malic, fumaric acids) always decreased. Interestingly, citric acid levels significantly correlated with the expression of some Fe and S deficiency induced genes. Our results contribute to existing knowledge on the complexity of the S/Fe interaction, suggesting a model in which endogenous alteration of citric acid content in plant tissues might act as signal molecule for the regulation of some nuclear-encoded and nutrient-responsive genes and also provide a basis for further study of the mechanism underlying S and Fe sensing and signalling.
Gianpiero Vigani; Youry Pii; Silvia Celletti; Mauro Maver; Tanja Mimmo; Stefano Cesco; Stefania Astolfi. Mitochondria dysfunctions under Fe and S deficiency: is citric acid involved in the regulation of adaptive responses? Plant Physiology and Biochemistry 2018, 126, 86 -96.
AMA StyleGianpiero Vigani, Youry Pii, Silvia Celletti, Mauro Maver, Tanja Mimmo, Stefano Cesco, Stefania Astolfi. Mitochondria dysfunctions under Fe and S deficiency: is citric acid involved in the regulation of adaptive responses? Plant Physiology and Biochemistry. 2018; 126 ():86-96.
Chicago/Turabian StyleGianpiero Vigani; Youry Pii; Silvia Celletti; Mauro Maver; Tanja Mimmo; Stefano Cesco; Stefania Astolfi. 2018. "Mitochondria dysfunctions under Fe and S deficiency: is citric acid involved in the regulation of adaptive responses?" Plant Physiology and Biochemistry 126, no. : 86-96.
Iron chlorosis is a serious deficiency that affects orchards and vineyards reducing quality and yield production. Chlorotic plants show abnormal photosynthesis and yellowing shoots. In grapevine iron uptake and homeostasis are most likely controlled by a mechanism known as “Strategy I,” characteristic of non-graminaceous plants and based on a system of soil acidification, iron reduction and transporter-mediated uptake. Nowadays, grafting of varieties of economic interest on tolerant rootstocks is widely used practice against many biotic and abiotic stresses. Nevertheless, many interspecific rootstocks, and in particular those obtained by crossing exclusively non-vinifera genotypes, can show limited nutrient uptake and transport, in particular for what concerns iron. In the present study, 101.14, a commonly used rootstock characterized by susceptibility to iron chlorosis was subjected to both Fe-absence and Fe-limiting conditions. Grapevine plantlets were grown in control, Fe-deprived, and bicarbonate-supplemented hydroponic solutions. Whole transcriptome analyses, via mRNA-Seq, were performed on root apices of stressed and unstressed plants. Analysis of differentially expressed genes (DEGs) confirmed that Strategy I is the mechanism responsible for iron uptake in grapevine, since many orthologs genes to the Arabidopsis “ferrome” were differentially regulated in stressed plant. Molecular differences in the plant responses to Fe absence and presence of bicarbonate were also identified indicating the two treatments are able to induce response-mechanisms only partially overlapping. Finally, we measured the expression of a subset of genes differentially expressed in 101.14 (such as IRT1, FERRITIN1, bHLH38/39) or known to be fundamental in the “strategy I” mechanism (AHA2 and FRO2) also in a tolerant rootstock (M1) finding important differences which could be responsible for the different degrees of tolerance observed.
Alessandro Vannozzi; Silvia Donnini; Gianpiero Vigani; Massimiliano Corso; Giorgio Valle; Nicola Vitulo; Claudio Bonghi; Graziano Zocchi; Margherita Lucchin. Transcriptional Characterization of a Widely-Used Grapevine Rootstock Genotype under Different Iron-Limited Conditions. Frontiers in Plant Science 2017, 7, 471 .
AMA StyleAlessandro Vannozzi, Silvia Donnini, Gianpiero Vigani, Massimiliano Corso, Giorgio Valle, Nicola Vitulo, Claudio Bonghi, Graziano Zocchi, Margherita Lucchin. Transcriptional Characterization of a Widely-Used Grapevine Rootstock Genotype under Different Iron-Limited Conditions. Frontiers in Plant Science. 2017; 7 ():471.
Chicago/Turabian StyleAlessandro Vannozzi; Silvia Donnini; Gianpiero Vigani; Massimiliano Corso; Giorgio Valle; Nicola Vitulo; Claudio Bonghi; Graziano Zocchi; Margherita Lucchin. 2017. "Transcriptional Characterization of a Widely-Used Grapevine Rootstock Genotype under Different Iron-Limited Conditions." Frontiers in Plant Science 7, no. : 471.
Molybdenum (Mo) and iron (Fe) are essential micronutrients required for crucial enzyme activities in plant metabolism. Here we investigated the existence of a mutual control of Mo and Fe homeostasis in cucumber (Cucumis sativus). Plants were grown under single or combined Mo and Fe starvation. Physiological parameters were measured, the ionomes of tissues and the ionomes and proteomes of root mitochondria were profiled, and the activities of molybdo-enzymes and the synthesis of molybdenum cofactor (Moco) were evaluated. Fe and Mo were found to affect each other's total uptake and distribution within tissues and at the mitochondrial level, with Fe nutritional status dominating over Mo homeostasis and affecting Mo availability for molybdo-enzymes in the form of Moco. Fe starvation triggered Moco biosynthesis and affected the molybdo-enzymes, with its main impact on nitrate reductase and xanthine dehydrogenase, both being involved in nitrogen assimilation and mobilization, and on the mitochondrial amidoxime reducing component. These results, together with the identification of > 100 proteins differentially expressed in root mitochondria, highlight the central role of mitochondria in the coordination of Fe and Mo homeostasis and allow us to propose the first model of the molecular interactions connecting Mo and Fe homeostasis.
Gianpiero Vigani; Dario Di Silvestre; Anna Maria Agresta; Silvia Donnini; Pierluigi Mauri; Christian Gehl; Florian Bittner; Irene Murgia. Molybdenum and iron mutually impact their homeostasis in cucumber ( Cucumis sativus ) plants. New Phytologist 2016, 213, 1222 -1241.
AMA StyleGianpiero Vigani, Dario Di Silvestre, Anna Maria Agresta, Silvia Donnini, Pierluigi Mauri, Christian Gehl, Florian Bittner, Irene Murgia. Molybdenum and iron mutually impact their homeostasis in cucumber ( Cucumis sativus ) plants. New Phytologist. 2016; 213 (3):1222-1241.
Chicago/Turabian StyleGianpiero Vigani; Dario Di Silvestre; Anna Maria Agresta; Silvia Donnini; Pierluigi Mauri; Christian Gehl; Florian Bittner; Irene Murgia. 2016. "Molybdenum and iron mutually impact their homeostasis in cucumber ( Cucumis sativus ) plants." New Phytologist 213, no. 3: 1222-1241.
Plant production and plant product quality strongly depends on the availability of mineral nutrients. Among them, sulfur (S) and iron (Fe) play a central role, as they are needed for many proteins of the respiratory chain. Plant mitochondria play essential bioenergetic and biosynthetic functions as well as they have an important role in signalling processes into the cell. Here, by comparing several transcriptomic data sets from plants impaired in their respiratory function with the genes regulated under Fe or S deficiencies obtained from other data sets, nutrient-responsive genes potentially regulated by hypothetical mitochondrial retrograde signalling pathway are evidenced. It leads us to hypothesize that plant mitochondria could be therefore required for regulating the expression of key genes involved both in Fe and S metabolisms.
Gianpiero Vigani; Jean-François Briat. Impairment of Respiratory Chain under Nutrient Deficiency in Plants: Does it Play a Role in the Regulation of Iron and Sulfur Responsive Genes? Frontiers in Plant Science 2016, 6, 1185 .
AMA StyleGianpiero Vigani, Jean-François Briat. Impairment of Respiratory Chain under Nutrient Deficiency in Plants: Does it Play a Role in the Regulation of Iron and Sulfur Responsive Genes? Frontiers in Plant Science. 2016; 6 ():1185.
Chicago/Turabian StyleGianpiero Vigani; Jean-François Briat. 2016. "Impairment of Respiratory Chain under Nutrient Deficiency in Plants: Does it Play a Role in the Regulation of Iron and Sulfur Responsive Genes?" Frontiers in Plant Science 6, no. : 1185.
Iron (Fe) is an essential micronutrient for plant growth and development, and its reduced bioavailability strongly impairs mitochondrial functionality. In this work, the metabolic adjustment in the rice (Oryza sativa) mitochondrial Fe transporter knockdown mutant (mit-2) was analysed. Biochemical characterization of purified mitochondria from rice roots showed alteration in the respiratory chain of mit-2 compared with wild-type (WT) plants. In particular, proteins belonging to the type II alternative NAD(P)H dehydrogenases accumulated strongly in mit-2 plants, indicating that alternative pathways were activated to keep the respiratory chain working. Additionally, large-scale changes in the transcriptome and metabolome were observed in mit-2 rice plants. In particular, a strong alteration (up-/down-regulation) in the expression of genes encoding enzymes of both primary and secondary metabolism was found in mutant plants. This was reflected by changes in the metabolic profiles in both roots and shoots of mit-2 plants. Significant alterations in the levels of amino acids belonging to the aspartic acid-related pathways (aspartic acid, lysine, and threonine in roots, and aspartic acid and ornithine in shoots) were found that are strictly connected to the Krebs cycle. Furthermore, some metabolites (e.g. pyruvic acid, fumaric acid, ornithine, and oligosaccharides of the raffinose family) accumulated only in the shoot of mit-2 plants, indicating possible hypoxic responses. These findings suggest that the induction of local Fe deficiency in the mitochondrial compartment of mit-2 plants differentially affects the transcript as well as the metabolic profiles in root and shoot tissues.
Gianpiero Vigani; Khurram Bashir; Yasuhiro Ishimaru; Martin Lehmann; Fabio Marco Casiraghi; Hiromi Nakanishi; Motoaki Seki; Peter Geigenberger; Graziano Zocchi; Naoko K. Nishizawa. Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants. Journal of Experimental Botany 2015, 67, 1357 -68.
AMA StyleGianpiero Vigani, Khurram Bashir, Yasuhiro Ishimaru, Martin Lehmann, Fabio Marco Casiraghi, Hiromi Nakanishi, Motoaki Seki, Peter Geigenberger, Graziano Zocchi, Naoko K. Nishizawa. Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants. Journal of Experimental Botany. 2015; 67 (5):1357-68.
Chicago/Turabian StyleGianpiero Vigani; Khurram Bashir; Yasuhiro Ishimaru; Martin Lehmann; Fabio Marco Casiraghi; Hiromi Nakanishi; Motoaki Seki; Peter Geigenberger; Graziano Zocchi; Naoko K. Nishizawa. 2015. "Knocking down mitochondrial iron transporter (MIT) reprograms primary and secondary metabolism in rice plants." Journal of Experimental Botany 67, no. 5: 1357-68.
Ferritins are iron-storage proteins involved in the environmental and developmental control of the free iron pool within cells. Plant ferritins are targeted to mitochondria as well as to chloroplasts. AtFer4 is the Arabidopsis thaliana ferritin isoform that can be also targeted to mitochondria. Frataxin is a mitochondrial protein whose role is essential for plants; lack of AtFH frataxin causes early embryo-lethality in Arabidopsis. Because of that, the Arabidopsis atfh KO mutant is propagated in heterozygosis. For exploring the functional interaction between frataxin and ferritin, Arabidopsis double mutant atfer4-1/atfh was isolated and its physiological parameters were measured, as well as its ionome profile, together with those of both atfer4 and atfh single mutants, in different conditions of Fe supply. Impairment of both ferritin and frataxin did not lead to any effect on mitochondrial respiration. However, ionomics revealed that the content of macro- and microelements, occurring when the nutritional Fe supply changes, were altered in the mutants analysed. These results suggest that both ferritin and frataxin can contribute to the composition of the leaf ionome and also confirm ionomics as an excellent tool for detecting alterations in the plant's physiology.
Irene Murgia; Gianpiero Vigani. Analysis of Arabidopsis thaliana atfer4-1, atfh and atfer4-1/atfh mutants uncovers frataxin and ferritin contributions to leaf ionome homeostasis. Plant Physiology and Biochemistry 2015, 94, 65 -72.
AMA StyleIrene Murgia, Gianpiero Vigani. Analysis of Arabidopsis thaliana atfer4-1, atfh and atfer4-1/atfh mutants uncovers frataxin and ferritin contributions to leaf ionome homeostasis. Plant Physiology and Biochemistry. 2015; 94 ():65-72.
Chicago/Turabian StyleIrene Murgia; Gianpiero Vigani. 2015. "Analysis of Arabidopsis thaliana atfer4-1, atfh and atfer4-1/atfh mutants uncovers frataxin and ferritin contributions to leaf ionome homeostasis." Plant Physiology and Biochemistry 94, no. : 65-72.