Software applications to solve engineering problems and performance issues of marine diesel engines are becoming easier in current trends due to the rapid development of technology and computer science. However, the cost of software kits for users is very high and burdensome, especially for researchers in developing countries. Alternatively, Diesel-RK software is an open-source program created to help researchers in modelling and making predictions on the performance and exhaust emissions of a diesel engine. Therefore, this work presents the results of the Diesel-RK application for simulating performance, emissions, and the capability of use of another type of diesel by marine diesel engines of 4-cycle-inlined 6-cylinder, and suggests the best type of diesel fuel for testing on an actual marine engine. Different types of diesel fuel, such as Light Fuel Oil 1 (LFO-1), Diesel No. 2, EN590, and Heavy Fuel Oil, were tested in this simulation. The engine performance and emission parameters, such as brake power, torque, specific fuel consumption, thermal efficiency, Nitrogen Oxide (NOx) emissions, and Carbon Dioxide (CO2) emissions, were analysed. The research indicated that utilising EN590 fuel resulted in enhanced marine diesel engine performance, including improved brake power, torque, thermal efficiency, and fuel consumption. Moreover, EN590 fuel exhibited significantly reduced NOx and CO2 emissions compared to Diesel No.2, LFO-1, and Heavy Fuel, offering valuable guidance for both simulation and practical engine studies.