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The present study explored the plant growth promotion and bioremediation potential of bacteria inhabiting wastewater irrigated agricultural soils. Thirty out of 75 bacterial isolates (40%), 29/75 (39%) and 28/75 (37%) solubilized Zn, K and PO4 during plate essays respectively. Fifty-six percent of the isolates produced siderophores, while 30% released protease in vitro. Seventy-four percent of bacteria resisted Pb, Ni and Cd at various concentrations added to the culture media plates. Sixteen out of 75 (26%) isolates were able to fix N in Nbf medium. Among these 16 N fixers, N fixing nifH, nifD and nifK genes was detected through PCR in 8, 7 and 1 strain respectively using gene specific primers designed in the study with Enterobacter sp. having all three (nifHKD) genes. Isolated bacteria showed resemblance to diverse genera such as Bacillus, Pseudomonas, Enterobacter, Citrobacter, Acinetobacter, Serratia, Klebsiella and Enterococcus based on 16S rRNA gene sequence analysis. In addition to showing the best mineral solubilization and metal resistance potential, Citrobacter sp. and Enterobacter sp. also removed 87%, 79% and 43% and 86%, 78% and 51% of Ni, Cd and Pb, respectively, from aqueous solution. These potent bacteria may be exploited both for bioremediation and biofertilization of wastewater irrigated soils leading to sustainable agriculture.
Abdul Ajmal; Saleha Saroosh; Shah Mulk; Muhammad Hassan; Humaira Yasmin; Zahra Jabeen; Asia Nosheen; Syed Shah; Rabia Naz; Zuhair Hasnain; Tariq Qureshi; Abdul Waheed; Saqib Mumtaz. Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits. Sustainability 2021, 13, 7792 .
AMA StyleAbdul Ajmal, Saleha Saroosh, Shah Mulk, Muhammad Hassan, Humaira Yasmin, Zahra Jabeen, Asia Nosheen, Syed Shah, Rabia Naz, Zuhair Hasnain, Tariq Qureshi, Abdul Waheed, Saqib Mumtaz. Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits. Sustainability. 2021; 13 (14):7792.
Chicago/Turabian StyleAbdul Ajmal; Saleha Saroosh; Shah Mulk; Muhammad Hassan; Humaira Yasmin; Zahra Jabeen; Asia Nosheen; Syed Shah; Rabia Naz; Zuhair Hasnain; Tariq Qureshi; Abdul Waheed; Saqib Mumtaz. 2021. "Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits." Sustainability 13, no. 14: 7792.
The growth and persistence of rhizobacteria in soils are highly impacted by moisture stress. In this study, we report the first transcript analysis of four Pseudomonas strains (PS1, PS2, PS3, and PS4) isolated from the root-soil interface of rice and maize associated with different moisture levels during water deprivation. Filtered Pseudomonas sp. cells incubated at low (RH10%) and high (RH85%) relative humidity showed decreased survival of all Pseudomonas sp. at RH10% when compared with RH85%. RT-PCR showed differential expression of treS (trehalose synthase), rpoS (sigma factor), mucA (alginate regulatory gene), and fliM (flagellar motor switch protein gene) in response to exposure to RH10%. However, molecular fingerprinting and nutrient assimilation profile of Pseudomonas strains demonstrated genetic and physiological variation between the four strains irrespective of water regime and host. In vitro testing of these strains showed ACC deaminase activity and gibberellic acid, abscisic acid, indole acetic acid, and exopolysaccharide production. We determined that 50 μl of 1.2 × 103 CFU ml-1 of these Pseudomonas strains was enough to protect Arabidopsis plants against drought stress in a pot experiment. Inoculated plants increased their root colonization ability and biomass; however, PS2 showed higher survival (95%), relative water content (59%), chlorophyll (30%), glycine betaine (38%), proline (23%), and reduced MDA (43%) in shoots than irrigated control under induced water deprivation. It can be concluded that all Pseudomonas strains were effective in mitigating drought stress, however, PS2 appears to impart more resistance to drought than the other strains by upregulating key defense mechanisms.
Humaira Yasmin; Asghari Bano; Neil L. Wilson; Asia Nosheen; Rabia Naz; Muhammad Nadeem Hassan; Noshin Ilyas; Muhammad Hamzah Saleem; Ahmed Noureldeen; Parvaiz Ahmad; Ivan Kennedy. Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana. Physiologia Plantarum 2021, 1 .
AMA StyleHumaira Yasmin, Asghari Bano, Neil L. Wilson, Asia Nosheen, Rabia Naz, Muhammad Nadeem Hassan, Noshin Ilyas, Muhammad Hamzah Saleem, Ahmed Noureldeen, Parvaiz Ahmad, Ivan Kennedy. Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana. Physiologia Plantarum. 2021; ():1.
Chicago/Turabian StyleHumaira Yasmin; Asghari Bano; Neil L. Wilson; Asia Nosheen; Rabia Naz; Muhammad Nadeem Hassan; Noshin Ilyas; Muhammad Hamzah Saleem; Ahmed Noureldeen; Parvaiz Ahmad; Ivan Kennedy. 2021. "Drought‐tolerant Pseudomonas sp. showed differential expression of stress‐responsive genes and induced drought tolerance in Arabidopsis thaliana." Physiologia Plantarum , no. : 1.
Tomato wilt caused by F. oxysporum (Fox) is one of the most destructive diseases, which causes qualitative and quantitative losses. In the present study, the bio-efficacy of glucanolytic bacteria was assessed on tomato against Fusarium wilt under net house conditions. A 178 rhizobacteria were isolated from the wheat-maize cropping system of arid and semi-arid regions. Only ten out of 178 rhizobacteria produced glucanases. The glucanolytic bacteria exhibited variable ability to produce β-1, 3/4-glucanases (0.11 – 0.30 U/mL). The glucanase production was higher at pH 7 (SZ =7.89 mm - 14.11 mm) followed by pH 9 (SZ=5.78 mm - 10.56 mm) and pH 5 (SZ=5.55 mm - 10.00 mm). The glucanolytic bacteria MAZ 10SR, 13, 18, 32, 48, 51, 73, 86, 117, 151 & 165 suppressed F. oxysporum (43–68%) causing tomato wilt and other phytopathogens viz F. moniliforme (44–63%), M. phaseolina (42–65%) and P. oryzae (42–67%). The glucanolytic bacteria also produced different antifungal metabolites/compounds viz siderophores, protease, and lipopeptide antibiotic surfactin. In planta assays indicated the ability of glucanolytic bacteria Bacillus subtilis MAZ 10SR, 51, 117 & 165 to colonize the tomato rhizosphere by 7.4 – 8.9 log CFU/g of soil. They decreased the Fusarium wilt incidence with disease severity of 22–31% as compared to that of control (disease severity = 56%). A significant quantity of glucanases (0.33–0.70 U/mg of soil) was recovered from the tomato rhizosphere inoculated with the effective glucanolytic bacteria. The glucanolytic rhizobacteria were identified as Achromobacter mucicolens, Enterobacter cloacae, Serratia marcescens, and Bacillus spp. by 16S rRNA gene analysis. The utilization of glucanolytic bacteria could be a worthy strategy in the biological control of soil-borne pathogens.
Muhammad Atif Zia; Raheela Riaz; Asiya Batool; Humaira Yasmin; Asia Nosheen; Rabia Naz; Muhammad Nadeem Hassan. Glucanolytic rhizobacteria associated with wheat- maize cropping system suppress the Fusarium wilt of tomato (Lycopersicum esculentum L). Scientia Horticulturae 2021, 287, 110275 .
AMA StyleMuhammad Atif Zia, Raheela Riaz, Asiya Batool, Humaira Yasmin, Asia Nosheen, Rabia Naz, Muhammad Nadeem Hassan. Glucanolytic rhizobacteria associated with wheat- maize cropping system suppress the Fusarium wilt of tomato (Lycopersicum esculentum L). Scientia Horticulturae. 2021; 287 ():110275.
Chicago/Turabian StyleMuhammad Atif Zia; Raheela Riaz; Asiya Batool; Humaira Yasmin; Asia Nosheen; Rabia Naz; Muhammad Nadeem Hassan. 2021. "Glucanolytic rhizobacteria associated with wheat- maize cropping system suppress the Fusarium wilt of tomato (Lycopersicum esculentum L)." Scientia Horticulturae 287, no. : 110275.
F. solani causing root rot is the most devastating pathogen of pea (P. sativum L). Biological control using plant growth promoting rhizobacteria has great potential in plant disease management. In the current study, 41 bacteria were isolated from the rhizosphere of different vegetables grown under variable cropping practices. Only two out of 41 rhizobacteria antagonized the root rot pathogen (45–65%) until the 15th day of dual culture assay. The antagonistic bacteria produced one or more of the antifungal metabolites viz siderophores (discolourization zone = 3.8 mm), protease (solubilization index = 2.2–2.3), glucanases (solubilization index = 4.6–5.2) and solubilized the zinc (solubilization index = 4.0–4.6) from the insoluble ore. The antagonistic bacteria were identified as Bacillus subtilis FZV-1 and B. halotolerans FZV 34 by 16S rRNA, pta, glp F, and pyr C gene analysis. The rhizobacteria also indicated putative production of lipopeptide antibiotics surfactin and fengycin based on the presence of sfp and fen D genes. In pea inoculation assays, rhizobacteria decreased the germination time over control (4–35%) and enhanced the shoot length (29–64%), root length (23–78%) and fresh weight (28–70%). The rhizobacteria also reduced the root rot severity over control (25–89%) in the pea varieties meteor and climax. The versatile plant growth promoting potential of these strains emphasizes their potential use in the biological management of pea root rot.
Raheela Riaz; Asghar Khan; Wajeeha Jahangir Khan; Zahra Jabeen; Humaira Yasmin; Rabia Naz; Asia Nosheen; Muhammad Nadeem Hassan. Vegetable associated Bacillus spp. suppress the pea (Pisum sativum L.) root rot caused by Fusarium solani. Biological Control 2021, 158, 104610 .
AMA StyleRaheela Riaz, Asghar Khan, Wajeeha Jahangir Khan, Zahra Jabeen, Humaira Yasmin, Rabia Naz, Asia Nosheen, Muhammad Nadeem Hassan. Vegetable associated Bacillus spp. suppress the pea (Pisum sativum L.) root rot caused by Fusarium solani. Biological Control. 2021; 158 ():104610.
Chicago/Turabian StyleRaheela Riaz; Asghar Khan; Wajeeha Jahangir Khan; Zahra Jabeen; Humaira Yasmin; Rabia Naz; Asia Nosheen; Muhammad Nadeem Hassan. 2021. "Vegetable associated Bacillus spp. suppress the pea (Pisum sativum L.) root rot caused by Fusarium solani." Biological Control 158, no. : 104610.
Controlling agricultural pests using suitable biocontrol agents has been considered the best strategy for sustainable agriculture. Charcoal rot caused by a necrotrophic fungus Macrophomina phaseolina is responsible for a 30–50% annual reduction in soybean yield worldwide. Little is known about the role of Bacillus clausii in reducing charcoal rot disease severity in the soybean crop. In this study, we investigated plant growth promoting and antagonistic potential of Pseudomonas putida (MT604992) and Bacillus clausii (MT604989) against charcoal rot disease incidence in soybean. Among twenty bacteria isolated from soil and water samples of two different hot springs of Gilgit-Baltistan, Pakistan, 80% were siderophore positive; 65% were hydrogen cyanide (HCN) positive; 55%, 30%, and 75% were phosphate, potassium, and zinc solubilizers, respectively. Based on higher antagonistic activities and plant growth promoting traits five strains were selected for in vitro screening. Out of all tested strains, Pseudomonas putida and Bacillus clausii showed a significant increase in germination, growth, and disease suppression in soybean. These strains produced a pronounced increase in relative water content, photosynthetic pigments, membrane stability, proline, antioxidant enzymes status, phytohormones content (Salicylic acid, and Jasmonic acid), and disease suppression in comparison to control plants. Bacillus clausii mitigated the disease by 97% with a marked increase in the proline content (73% and 89%), superoxide dismutase (356% and 208%), peroxidase (439% and 138.6%), catalase (255.8% and 80.8%), and ascorbate peroxidase (228% and 90%) activities in shoots and roots, respectively. Infected plants showed an increase in salicylic acid and jasmonic acid content which was further increased with the application of the selected strains to increase resistance against pathogens. To our knowledge, this is the first study showing a rise in salicylic acid and jasmonic acid in Macrophomina phaseolina infected plants. These two strains are suggested as a cost-effective, eco-friendly, and sustainable alternative to chemical fungicides. However, there is a need to explore the field testing and molecular mechanisms leading to disease suppression by these strains.
Humaira Yasmin; Rabia Naz; Asia Nosheen; Muhammad Hassan; Noshin Ilyas; Muhammad Sajjad; Seemab Anjum; Xiangkuo Gao; Zhide Geng. Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina Phaseolina in Soybean (Glycine Max L.). Sustainability 2020, 12, 6856 .
AMA StyleHumaira Yasmin, Rabia Naz, Asia Nosheen, Muhammad Hassan, Noshin Ilyas, Muhammad Sajjad, Seemab Anjum, Xiangkuo Gao, Zhide Geng. Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina Phaseolina in Soybean (Glycine Max L.). Sustainability. 2020; 12 (17):6856.
Chicago/Turabian StyleHumaira Yasmin; Rabia Naz; Asia Nosheen; Muhammad Hassan; Noshin Ilyas; Muhammad Sajjad; Seemab Anjum; Xiangkuo Gao; Zhide Geng. 2020. "Identification of New Biocontrol Agent against Charcoal Rot Disease Caused by Macrophomina Phaseolina in Soybean (Glycine Max L.)." Sustainability 12, no. 17: 6856.
Fusarium root and crown rot is the most common disease of wheat, especially wheat grown in arid zones where drought is a common issue. The development of environmentally safe approaches to manage diseases of food crops is important for humans. The monocropping system recruits beneficial bacteria that promote plant growth through nutrient solubilization and pathogen suppression. In this study, a field where wheat was monocropped for 5 successive years under rainfed conditions was identified. A total of 29 bacterial isolates were obtained from the rhizosphere, endosphere, and phyllosphere of wheat at its harvesting stage. The Gram-negative bacteria were less prevalent (41%) but the majority (75%) exhibited plant growth-promoting traits. The ability of strains to solubilize nutrients (solubilization index = 2.3 to 4), inhibit pathogenic fungi (25 to 56%), and produce antifungal compounds was highly variable. The rhizobacteria significantly promoted the growth and disease resistance of wheat varieties such as Pirsbak-2015 and Galaxy-2013 by inducing antioxidant enzyme activity (0.2- to 2.1-fold). The bacterial strains were identified as Ochrobactrum spp., Acinetobacter spp., and Pseudomonas mediterranea by 16S rRNA and rpoD sequence analysis. The endophytic bacterium P. mediterranea HU-9 exhibited maximum biocontrol efficacy against wheat root and crown rot diseases with a disease score/disease index from 1.8 to 3.1. The monocropping systems of rainfed agriculture are an ideal source of beneficial bacteria to use as bioinoculants for different crops.
Habib Ullah; Humaira Yasmin; Saqib Mumtaz; Zahra Jabeen; Rabia Naz; Asia Nosheen; Muhammad Nadeem Hassan. Multitrait Pseudomonas spp. Isolated from Monocropped Wheat (Triticum aestivum) Suppress Fusarium Root and Crown Rot. Phytopathology® 2020, 110, 582 -592.
AMA StyleHabib Ullah, Humaira Yasmin, Saqib Mumtaz, Zahra Jabeen, Rabia Naz, Asia Nosheen, Muhammad Nadeem Hassan. Multitrait Pseudomonas spp. Isolated from Monocropped Wheat (Triticum aestivum) Suppress Fusarium Root and Crown Rot. Phytopathology®. 2020; 110 (3):582-592.
Chicago/Turabian StyleHabib Ullah; Humaira Yasmin; Saqib Mumtaz; Zahra Jabeen; Rabia Naz; Asia Nosheen; Muhammad Nadeem Hassan. 2020. "Multitrait Pseudomonas spp. Isolated from Monocropped Wheat (Triticum aestivum) Suppress Fusarium Root and Crown Rot." Phytopathology® 110, no. 3: 582-592.
Plant growth-promoting bacteria have broad range usage in agriculture as an alternative to chemical fertilizers and fungicides. Their survival and persistency in the plant vicinity depend upon the production of secondary metabolites. In present study, 45 bacteria were isolated from the rhizosphere and endosphere of different sugarcane varieties growing at the different farmer’s fields of Punjab, Pakistan. Out of 45 isolates, 3 were able to produce hydrolytic enzyme glucanase. The glucanase-producing ability of bacterial strains was variable with solubilization zone 5.5–13.8 mm of different substrates. Dinitrosalicylic acid (DNS) quantification depicted the production of glucanase (0.1–0.3 U/mL of culture filtrate) by the antagonistic strains. All the glucanase-producing bacteria significantly inhibited the economically important pathogens of sugarcane, i.e., Fusarium moniliforme (45–56%) and Colletotrichum falcatum (52–63%), and pathogens of other crops, i.e., Fusarium oxysporum (58–63%), Rhizoctonia solani (42–53%) and Macrophomina phaseolina (53–61%). The glucanase-producing bacteria significantly induced the activities of enzymes involved in ROS scavenging, viz. SOD, POD, CAT and PPO by 1.4–2.0-fold. The glucanase-producing bacteria MAZ-3SR was identified as Bacillus amyloliquefaciens, while MAZ-10SR and MAZ-29SR were identified as Bacillus subtilis by 16S rDNA sequence comparision. These glucanolytic bacteria could be effectively used in sugarcane disease management and provide an effective way of enumerating the bioantagonists from sugarcane rhizosphere.
Muhammad Atif Zia; Humaira Yasmin; Faluk Shair; Zahra Jabeen; Saqib Mumtaz; Zafar Hayat; Syed Zia Ul Husnain Shah; Shahid Afghan; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. Glucanolytic Rhizobacteria Produce Antifungal Metabolites and Elicit ROS Scavenging System in Sugarcane. Sugar Tech 2018, 21, 244 -255.
AMA StyleMuhammad Atif Zia, Humaira Yasmin, Faluk Shair, Zahra Jabeen, Saqib Mumtaz, Zafar Hayat, Syed Zia Ul Husnain Shah, Shahid Afghan, Fauzia Yusuf Hafeez, Muhammad Nadeem Hassan. Glucanolytic Rhizobacteria Produce Antifungal Metabolites and Elicit ROS Scavenging System in Sugarcane. Sugar Tech. 2018; 21 (2):244-255.
Chicago/Turabian StyleMuhammad Atif Zia; Humaira Yasmin; Faluk Shair; Zahra Jabeen; Saqib Mumtaz; Zafar Hayat; Syed Zia Ul Husnain Shah; Shahid Afghan; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. 2018. "Glucanolytic Rhizobacteria Produce Antifungal Metabolites and Elicit ROS Scavenging System in Sugarcane." Sugar Tech 21, no. 2: 244-255.
Rice blast is a severe threat for agricultural production. Plant growth promoting rhizobacteria could be suitable biocontrol agents to reduce the disease incidence. In this study, Bacillus spp. KFP-5, KFP-7, KFP-17 significantly reduced disease severity by 40–52% with grain yield of 3.2–3.9 ton ha−1 in two rice varieties i.e., basmati super and basmati 385. Bacillus spp. significantly colonized the rice rhizosphere with a cell population of 2.40E+06–5.6E+07CFU. Rice plants treated with antagonistic bacterial suspension followed by challenge inoculation with P. oryzae were found to have higher activities of antioxidant enzymes such as superoxide dismutase (308–266 Ug−1 FW), peroxidase (change in absorbance (ΔA) = 0.20–0.71 min−1 g−1 FW), polyphenol oxidase (ΔA = 0.29–0.58 min−1 g−1 FW) and phenylalanine ammonia lyase (ΔA = 0.32–0.59 min−1 g−1 FW). A consistency in the performance of strains was observed in the consecutive years 2013–2014. These findings suggest that indigenous Bacillus spp. could be a potential bio-inoculum for rice to control blast diseases and enhance yield.
Afroz Rais; Muhammad Shakeel; Kamran Malik; Fauzia Yusuf Hafeez; Humaira Yasmin; Saqib Mumtaz; Muhammad Nadeem Hassan. Antagonistic Bacillus spp. reduce blast incidence on rice and increase grain yield under field conditions. Microbiological Research 2018, 208, 54 -62.
AMA StyleAfroz Rais, Muhammad Shakeel, Kamran Malik, Fauzia Yusuf Hafeez, Humaira Yasmin, Saqib Mumtaz, Muhammad Nadeem Hassan. Antagonistic Bacillus spp. reduce blast incidence on rice and increase grain yield under field conditions. Microbiological Research. 2018; 208 ():54-62.
Chicago/Turabian StyleAfroz Rais; Muhammad Shakeel; Kamran Malik; Fauzia Yusuf Hafeez; Humaira Yasmin; Saqib Mumtaz; Muhammad Nadeem Hassan. 2018. "Antagonistic Bacillus spp. reduce blast incidence on rice and increase grain yield under field conditions." Microbiological Research 208, no. : 54-62.
Plant growth promoting rhizobacteria (PGPR) are found to control the plant diseases by adopting various mechanisms. Induced systemic resistance (ISR) is an important defensive strategy manifested by plants against numerous pathogens especially infecting at aerial parts. Rhizobacteria elicit ISR by inducing different pathways in plants through production of various metabolites. In the present study, potential of Bacillus spp. KFP-5, KFP-7, KFP-17 was assessed to induce antioxidant enzymes against Pyricularia oryzae infection in rice. The antagonistic Bacillus spp. significantly induced antioxidant defense enzymes i-e superoxide dismutase (1.7–1.9-fold), peroxidase (3.5–4.1-fold), polyphenol oxidase (3.0–3.8-fold), phenylalanine ammonia-lyase (3.9–4.4-fold), in rice leaves and roots under hydroponic and soil conditions respectively. Furthermore, the antagonistic Bacillus spp significantly colonized the rice plants (2.0E+00–9.1E+08) and secreted multiple biocontrol determinants like protease (1.1–5.5 U/mg of soil or U/mL of hydroponic solution), glucanase, (1.0–1.3 U/mg of soil or U/mL of hydroponic solution), siderophores (6.5–42.8 μg/mL or mg) in the rhizosphere of different rice varieties. The results showed that treatment with Bacillus spp. enhanced the antioxidant defense activities in infected rice, thus alleviating P. oryzae induced oxidative damage and suppressing blast disease incidence.
Afroz Rais; Zahra Jabeen; Faluk Shair; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLOS ONE 2017, 12, e0187412 -e0187412.
AMA StyleAfroz Rais, Zahra Jabeen, Faluk Shair, Fauzia Yusuf Hafeez, Muhammad Nadeem Hassan. Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLOS ONE. 2017; 12 (11):e0187412-e0187412.
Chicago/Turabian StyleAfroz Rais; Zahra Jabeen; Faluk Shair; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. 2017. "Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae." PLOS ONE 12, no. 11: e0187412-e0187412.
Macrophomina phaseolina is the most devastating pathogen which cause charcoal rot and root rot disease in various economically important crops. Three strains M. Phaseolina1156, M. Phaseolina1160 and M. Phaseolina PCMC/F1 were tested for their virulence on sunflower (Helianthus annuus L.) and chickpea (Cicer arietinum L).The strains showed high virulence on both hosts with a disease score of 2 on chickpea and sunflower. The strains also increased the hydrogen per oxide (H2O2) content by 1.4-1.6 fold in root as well as shoot of chickpea and sunflower. A significant increase in antioxidant enzymes was observed in fungal infected plants which indicated prevelance of oxidative stress during pathogen propagation. The M. phaseolina strains also produced hydrolytic enzymes such as lipase, amylase and protease with solubilization zone of 5-43mm, 5-45mm and 12-35 mm respectively. The M. Phaseolina strains were identified by 18S rRNA and analyzed for genetic diversity by using random amplified polymorphic DNA (RAPD) markers. The findings based on RAPD markers and 18S rRNA sequence analysis clearly indicate genetic variation among the strains collected from different hosts. The genetically diverse strains were found to be pathogenic to sunflower and chickpea.
Ali N. Khan; Faluk Shair; Kamran Malik; Zafar Hayat; Muhammad Ayub Khan; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. Molecular Identification and Genetic Characterization of Macrophomina phaseolina Strains Causing Pathogenicity on Sunflower and Chickpea. Frontiers in Microbiology 2017, 8, 1309 -1309.
AMA StyleAli N. Khan, Faluk Shair, Kamran Malik, Zafar Hayat, Muhammad Ayub Khan, Fauzia Yusuf Hafeez, Muhammad Nadeem Hassan. Molecular Identification and Genetic Characterization of Macrophomina phaseolina Strains Causing Pathogenicity on Sunflower and Chickpea. Frontiers in Microbiology. 2017; 8 ():1309-1309.
Chicago/Turabian StyleAli N. Khan; Faluk Shair; Kamran Malik; Zafar Hayat; Muhammad Ayub Khan; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. 2017. "Molecular Identification and Genetic Characterization of Macrophomina phaseolina Strains Causing Pathogenicity on Sunflower and Chickpea." Frontiers in Microbiology 8, no. : 1309-1309.
Plant growth promoting rhizobacteria are being widely utilized in agriculture due to their biopesticide and biofertilizer potential. The indigenous PGPR inhabiting certain hosts can act as potent bioinoculants due to their native habitat and acclimatization to the environment. Four hundred and fifty indigenous isolates were obtained from rice rhizosphere to assess their antagonism towards rice blast pathogen P. oryzae. Only 100 isolates showed antagonistic activity. Twenty out of 100 isolates inhibited the mycelium growth of P. oryzae by 30–65 % in vitro. Five strains, KFP-5, KFP-7, KFP-12, KFP-17 and KFP-18, showing 63–65 % antagonism towards P. oryzae, were tested for the production of biocontrol determinants and suppression of blast disease in rice plants. The antagonistic strains produced various biocontrol determinants such as the hydrolytic enzymes, protease, glucanase and cellulase with solubilization zone diameters of 10–19, 4.2–14.5 and 2–13.5 mm respectively and siderophores with zone diameter of 7–12 mm. The antagonistic strains also solubilized potassium, phosphorus and zinc from inorganic sources with solubilization zones of 2.1–3.2, 2.3–3.6 and 1.7-2.9 mm respectively. Three antagonistic strains, KFP-5, KFP-7 and KFP-17 significantly suppressed blast disease with a disease score 1.1–1.9 and increased yield of rice plants. The potent antagonistic strains were identified as members of the genus Bacillus by 16S rRNA gene sequencing.
Afroz Rais; Muhammad Shakeel; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. Plant growth promoting rhizobacteria suppress blast disease caused by Pyricularia oryzae and increase grain yield of rice. BioControl 2016, 61, 769 -780.
AMA StyleAfroz Rais, Muhammad Shakeel, Fauzia Yusuf Hafeez, Muhammad Nadeem Hassan. Plant growth promoting rhizobacteria suppress blast disease caused by Pyricularia oryzae and increase grain yield of rice. BioControl. 2016; 61 (6):769-780.
Chicago/Turabian StyleAfroz Rais; Muhammad Shakeel; Fauzia Yusuf Hafeez; Muhammad Nadeem Hassan. 2016. "Plant growth promoting rhizobacteria suppress blast disease caused by Pyricularia oryzae and increase grain yield of rice." BioControl 61, no. 6: 769-780.
Red rot disease affects sugarcane production adversely throughout the world. Intensive cropping and uninterrupted use of chemicals results in evolution of new pathotypes which can infect the resistant cultivars. Bacillus sp. inhabiting plant rhizosphere can protect the plants from multiple pathogens. In the present study, two antagonistic strains Bacillus subtilis NH-100 and Bacillus sp. NH-217 were able to maintain their population at 9.0 log CFU g−1 in sugarcane filter cake until nine months. These strains were compatibile to the indigenous bacteria of sugarcane filter cake which maintained their population at 7.8–8.0 log CFU g−1. The developed bioformulation induced the activity of various defense-related enzymes (0.6–6.9 change in absorbance min−1 g−1 of tissue), suppressed the red rot disease and enhanced crop yield under field conditions. This formulation could be used as potential biopesticide to control red rot disease of sugarcane.
Muhammad Nadeem Hassan; Namood- E- Sahar; Syed Zia-Ul-Husnain Shah; Shahid Afghan; Fauzia Yusuf Hafeez. Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake. BioControl 2015, 60, 691 -702.
AMA StyleMuhammad Nadeem Hassan, Namood- E- Sahar, Syed Zia-Ul-Husnain Shah, Shahid Afghan, Fauzia Yusuf Hafeez. Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake. BioControl. 2015; 60 (5):691-702.
Chicago/Turabian StyleMuhammad Nadeem Hassan; Namood- E- Sahar; Syed Zia-Ul-Husnain Shah; Shahid Afghan; Fauzia Yusuf Hafeez. 2015. "Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake." BioControl 60, no. 5: 691-702.
Muhammad Nadeem Hassan; Shahid Afghan; Fauzia Yusuf Hafeez. Erratum to: Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria. BioControl 2010, 55, 695 -695.
AMA StyleMuhammad Nadeem Hassan, Shahid Afghan, Fauzia Yusuf Hafeez. Erratum to: Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria. BioControl. 2010; 55 (5):695-695.
Chicago/Turabian StyleMuhammad Nadeem Hassan; Shahid Afghan; Fauzia Yusuf Hafeez. 2010. "Erratum to: Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria." BioControl 55, no. 5: 695-695.
Bacterial strains with ability to suppress Colletotrichum falcatum were isolated from the rhizosphere of sugarcane. Thirty nine candidates, chosen on the basis of in vitro antagonism, inhibited C. falcatum growth by 15–65% on test plates. Twenty two isolates causing 50% or more in vitro inhibition were screened for their root colonization ability and biocontrol activity on micropropagated sugarcane plants under greenhouse conditions. Twelve strains suppressed red rot infection in plantlets, but no significant correlation was observed between in vitro pathogen inhibition and in vivo disease suppression. However, isolates showing root colonization over 5.2 log10 CFU g−1 of soil showed highest suppression of C. falcatum and reduction of red rot disease. Six strains with the capability to maintain a significant population in the sugarcane rhizosphere and with a high potential to control red rot were identified by 16S rDNA as Ochrobacterum intermedium NH-5, Pseudomonas putida NH-50, Bacillus subtilis NH-100, Bacillus subtilis NH-160, Bacillus sp NH-217 and Stenotrophomonas maltophilia NH-300.
Muhammad Nadeem Hassan; Shahid Afghan; Fauzia Yusuf Hafeez. Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria. BioControl 2010, 55, 531 -542.
AMA StyleMuhammad Nadeem Hassan, Shahid Afghan, Fauzia Yusuf Hafeez. Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria. BioControl. 2010; 55 (4):531-542.
Chicago/Turabian StyleMuhammad Nadeem Hassan; Shahid Afghan; Fauzia Yusuf Hafeez. 2010. "Suppression of red rot caused by Colletotrichum falcatum on sugarcane plants using plant growth-promoting rhizobacteria." BioControl 55, no. 4: 531-542.