Herein, the amino-capped TiO₂ nanoparticles were synthesized using tetrabutyl titanate and amino polymers by a two-step sol-gel and hydrothermal method technique for the fabrication of functional cotton fabric. The prepared TiO₂ nanoparticles and the treated cotton fabric were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD), field emission scanning electron microcopy (FE-SEM) photocatalytic and antibacterial measurement. The results indicate the typical characteristic anatase form of the amino-capped TiO₂ NPs with an average crystallite size of 14.9 nm. The treated cotton fabrics exhibit excellent antibacterial property and good photocatalytic degradation of methylene blue.