Background: Infectious diseases are considered a global public health problem, with viruses being the predominant infectious agents afflicting the human population. The most used control alternatives are the search and development of vaccines and drugs. Nevertheless, their efficacy has limitations related to the immune response stimulation, resistance mechanisms, costeffort ratio, development, and production. An alternative to these drawbacks is the search for compounds isolated from plants with antiviral and/or virucidal properties. The genus Phyllanthus is a plant group producing compounds that gathers an antiviral and virucidal spectrum on different biological models. However, there is no complete review of their properties against viruses in cell models. Objective: To compile and analyse the more relevant information on the antiviral and virucidal activity in cell models, phytochemical composition, and generalities of the genus Phyllanthus. Method: The information was assembled from a general search for articles in various databanks, and the information was organized, tabulated, and discussed. Results: The taxonomic classification of the genus Phyllanthus showed discrepancies between different authors and publications. The antiviral and virucidal effects of Phyllanthus naturallyoccurring compounds on cell models showed a broad spectrum and a high chemical diversity mainly related to phenols and polyphenols. Conclusion: Antiviral and virucidal properties of Phyllanthus-derived compounds showed promising results as controlling agents against viral infections in different cell models, particularly in the viral replication and translation processes. Further studies are required to elucidate the specific mechanisms involved in these natural alternatives to expand their efficient and effective applications.

Background/Objectives: Phyllanthus species are renowned in traditional medicine for their diverse therapeutic properties, including potential anticancer activities. This study explored the antiproliferative potential of six Phyllanthus species by integrating chemical composition with bioactivity assays to identify key antiproliferative compounds. Methods: The integration of liquid chromatography–mass spectrometry (LC-MS)-based chemical composition data with antiproliferative activity against three cancer cell lines—PC-3 (prostate adenocarcinoma), SiHa (cervical carcinoma), and A549 (lung carcinoma)—as well as a normal mouse fibroblast line (L929) was performed by covariate analysis. These compounds were subsequently isolated and structurally characterized using spectroscopic methods. Results: Through covariate statistics, seven m/z features were found to be plausible active compounds, and after isolation, they were related to butyrolactone and arylnaphthalide lignans. Among the active isolates, an unreported compound, (+)-phyllanlathyrin 6, was discovered in the aerial part of Phyllanthus lathyroides. The isolated compounds exhibited moderate to good antiproliferative activity (IC₅₀ < 20 µM) with selectivity to SiHa, validating the covariate-based identification approach. Conclusions: These findings highlight the potential of Phyllanthus species as sources of novel anticancer agents, with specific arylnaphthalide lignans showing promising cytotoxic effects that could be further developed into therapeutic leads. Additionally, this study underscores the value of combining advanced analytical techniques with bioactivity testing to uncover bioactive compounds from natural sources. The results contribute to the growing body of evidence supporting the therapeutic relevance of Phyllanthus species and provide a foundation for future drug development efforts targeting cancer treatment.

Introducción. El sistema nervioso es uno de los blancos del virus del Zika. Es fundamental disponer de reactivos biológicos para apoyo diagnóstico e investigaciones sobre patogénesis que requieren tejidos fijados en aldehídos.Objetivo. Determinar la utilidad de dos antisueros elaborados para la detección de antígenos del virus del Zika en células y tejidos fijados en aldehídos.Materiales y métodos. Los antisueros se produjeron en conejos Nueva Zelanda a partir de un aislamiento viral del brote de Zika del 2016 en Colombia. En uno de los animales se empleó adyuvante de Freund para los refuerzos y, en el otro, el adyuvante Montanide. Se realizaron titulaciones por inmunorreacciones en células y tejidos positivos para el virus del Zika. Se emplearon los métodos de estreptavidina-fosfatasa alcalina y avidina-biotinaperoxidasa.Los antisueros se evaluaron en muestras infectadas experimentalmente para el virus del Zika y para otros virus relacionados y no relacionados; también, se evaluaron con muestras de otras técnicas utilizadas en el laboratorio, como cortes obtenidos con vibrátomo, muestras para microscopía óptica de alta resolución y microscopía electrónica de transmisión.Resultados. Hubo inmunorreactividad a los antígenos del virus del Zika con ambos antisueros, principalmente en las neuronas de la corteza cerebral. Se demostró inmunotinción en las muestras de cultivos celulares y en los tejidos de animales infectados experimentalmente. Hubo inmunorreactividad cruzada con los virus del dengue y de la fiebre amarilla, pero no con el virus de la rabia.