Biostimulants are gaining growing importance among preparations used for plant production. They may cause increasing the effectiveness of nutrient uptake. The aim of the study was to assess P and K accumulation by winter rapeseed after the application of a biostimulant under conditions of varied NPK and S fertilization levels. The field experiment was established on Alfisol, and the factors were: Two levels of NPK fertilization (high 180 N, 70 P, 132 K (kg ha⁻¹) or low 144 N, 35 P, 66 K (kg ha⁻¹)); elementary S fertilization (36 or 0 kg ha⁻¹) and application of seaweed biostimulant or without that treatment. Biostimulant caused an increase in P and K accumulation in the shoots of rapeseed during generative development. Application of biostimulant in rapeseed fertilized with lower NPK rates or not fertilized with S increased P and K accumulation in shoots to the level obtained at higher NPK and S rates without that treatment. Increased level of NPK fertilization caused an increase in P and K accumulation in both shoots and roots at flowering and ripening stages. At the fruit development stage, under higher NPK fertilization there was higher uptake of P and K in rapeseed shoots after presowing fertilization with S.

The present study has aimed at enhancing the insufficient knowledge of functional soil enzymes properties influenced by inorganic fertilization and biostimulant application to increase the uptake of nitrogen affecting the winter oilseed rape yield. Field experiments were conducted in Poland (53° N, 18° E) in Alfisol (USDA). In this experiment, the NPK rates applied were as follows: high 180 N, 70 P and 160 K 132 N (kg ha⁻¹) or low 144 N, 35 P and 66 K (kg ha⁻¹); fertilization with elemental S 36 or 0 (kg ha⁻¹); and the seaweed biostimulant Kelpak was applied or there was no such treatment. Due to low NPK fertilization rates, the activity of dehydrogenases, peroxidases, and catalase increased in subsistent generative development stages from flowering to ripening. At the ripening stage, the activity of these enzymes, as well as nitroreductase activity, were inhibited by high NPK fertilizer rates. The seaweed biostimulant application and S fertilization increased N accumulation in plants of oilseed rape in generative development, by 16% and 13%, respectively, as compared with the lack of these treatments. The application of S increased the uptake of nitrogen in shoots and in whole oilseed rape plants only after application of higher rates of NPK.

In 2019, 71 years after the establishment of a static fertiliser experiment, the chemical and biological properties of Luvisol soil with sandy-loam grain-size composition were determined. Soil samples were taken from six fertilised treatments: half-dose nitrogen, phosphorus and potassium in mineral fertilisers (½ NPK); full-dose nitrogen, phosphorus, potassium (NPK); manure fertilisation + nitrogen, phosphorus, potassium, magnesium and liming (FYM NPK Mg Ca); manure + mineral fertilisers without magnesium and liming (FYM NPK); manure + nitrogen and phosphorus (FYM NP); manure + nitrogen and potassium (FYM NK). The soil was tested in two layers at depths of 0–20 cm and 20–40 cm. Soil samples were tested for: pH in 1 M KCl (pH); electrical conductivity (EC); organic carbon content (OC); content of available phosphorus (Pa), potassium (Ka), magnesium (Mga) and sulphate sulphur (S-SO4); total number of bacteria (Bt), cellulolytic microorganisms (Bc), fungi (Ff) and actinomycetes (Ac); and alkaline phosphatase (AlP), acid phosphatase (AcP) and arylsulphatase (ArS) activity. The fertilisation that most favourably affected the chemical and biological properties of the soil was FYM NPK Mg Ca. This fertilisation increased: pH and EC; OC, Ka and Mga contents; Bt and Bc abundance; and AlP activity relative to all the methods of mineral and organic–mineral fertilisation that did not include all the ingredients of mineral fertilisers. On the other hand, the least favourable soil properties were formed by ½ NPK fertilisation in the 0–20 cm layer, and by the long-term use of mineral fertilisers only in the 20–40 cm layer.

The aim of the study was to investigate the effect of varying rates of FYM (0, 20, 40, 60 Mg ha⁻¹) and nitrogen N₀, N₁, N₂, and N₃ on the content of sulphate sulphur (VI) and the activity of arylsulphatase, which participates in the transformations of this element in Haplic Luvisol. The study report is based on a long-term field experiment with two different crop rotations: A – recognized as exhausting the humus from soil and B – recognized as enriching the soil with humus. During the cultivation of the plants, the soil was sampled four times from corn and a red clover cultivar and grass. The FYM fertilization rate for which the highest arylsulphatase activity and the content of sulphates were identified was 60 Mg ha⁻¹. An inhibitory effect of high rates (90 and 135 kg N ha⁻¹) of ammonium nitrate on the arylsulphatase activity was also observed. A significant correlation between the content of carbon, nitrogen, and sulphates and the arylsulphatase activity was recorded. The investigation on the effect of combined application of farmyard manure and mineral nitrogen fertilization on the activity of arylsulphatase participating in the sulphur cycling was launched to examine the problem in detail.

Besides nitrogen, phosphorus and potassium, sulphur is nowadays considered fundamental for plants, and due to its deficit in many regions, should be provided to crops by fertilization. The enzymes,...

Chronic diseases increasingly reflect a shared biological origin: persistent cellular stress. This review summarizes the biochemical mechanisms that normally preserve cellular homeostasis, namely redox regulation, endoplasmic reticulum proteostasis, mitochondrial quality control, autophagy, and DNA damage response, and explains how they fail under sustained lifestyle-related overload. Repeated exposure to psychological stress, sleep disruption, hypercaloric intake, and physical inactivity shifts adaptive signaling toward maladaptation, promoting oxidative damage, protein misfolding, mitochondrial dysfunction, low-grade inflammation, and genomic instability. These interconnected processes contribute to the development and progression of major chronic non-communicable diseases, including obesity, type 2 diabetes, cardiovascular disease, neurodegeneration, and cancer. Particular emphasis is placed on circadian and neuroendocrine regulation, especially overactivation of the hypothalamic–pituitary–adrenal axis and impaired nocturnal regenerative pathways such as glymphatic clearance and DNA repair. Together, the evidence supports a unifying model in which chronic pathology emerges from cumulative failure of cellular resilience systems rather than isolated organ-specific defects. This perspective highlights sleep optimization, stress reduction, and metabolic regulation as mechanistically grounded strategies for prevention and supportive interventions for chronic disease.