This page has only limited features, please log in for full access.
Selaginella tamariscina is a medicinal plant that contains a variety of plant secondary metabolites; however, it is currently being collected indiscriminately from its native habitats. Hence, we have developed an efficient propagation method for S. tamariscina. Explants grown in vitro were cultured in Murashige and Skoog medium of various strengths (1/16–2x), and the highest number of sporophytes (65.7) were obtained with 1/4x MS medium. Culturing explants at various lengths (3–12 mm) for 12 weeks indicated 12 mm as the most appropriate size for sporophyte propagation. We then evaluated various concentrations of individual components, sucrose (0–5%), total nitrogen (7.5–30 mM), nitrogen ratio (3:0–0:3), and agar (0.6–0.8%), in the 1/4x MS medium for explant growth for 12 weeks. The maximum number of sporophytes were formed in media containing 3% sucrose, 15 mM nitrogen, and 0.6% agar, with a nitrogen ratio of 1:2. The propagated S. tamariscina was then acclimatized in a controlled environment to improve survival in an external environment. These results demonstrate the effective conditions for in vitro mass propagation of S. tamariscina, finding that methods utilizing sporophytes were more efficient than conventional propagation methods and yielded numerous plants in a short period.
Kyungtae Park; Bo Jang; Ha Lee; Ju Cho; Cheol Lee. Effective Propagation of Selaginella tamariscina through Optimized Medium Composition. Agronomy 2021, 11, 578 .
AMA StyleKyungtae Park, Bo Jang, Ha Lee, Ju Cho, Cheol Lee. Effective Propagation of Selaginella tamariscina through Optimized Medium Composition. Agronomy. 2021; 11 (3):578.
Chicago/Turabian StyleKyungtae Park; Bo Jang; Ha Lee; Ju Cho; Cheol Lee. 2021. "Effective Propagation of Selaginella tamariscina through Optimized Medium Composition." Agronomy 11, no. 3: 578.
Bracken fern (Pteridium aquilinum var. latiusculum (Desv.) Underw. ex A. Heller) has long been grown industrially in South Korea. Conventional propagation methods, including planting rhizomes and in vitro seedling culture, are labor intensive and expensive, and thus not commercially suitable. We aimed to develop a system to produce synthetic seeds using fern spores (SFS). Synthetic seeds were prepared by mixing bracken spores and alginate matrix. Spore germination and gametophyte and sporophyte growth and development from SFS proceeded normally. Spore density affected gametophyte and sporophyte numbers. SFS prepared using cold (4 °C) long-term storage spores (even 7-year-old spores) could effectively form sporophytes. The highest germination was observed at 25 °C. Soaking-treated SFS successfully formed sporophytes, even after 30 days of storage at 4 °C; indeed, sporophytes formed even after five days of storage at 25 °C during transport conditions. SFS were sown in plug trays for commercial use. Young sporophytes grown from plug seedlings were greenhouse cultivated, and transplanting within eight weeks was effective for root growth and growing-point formation. Developing synthetic seeds is a feasible solution for facilitating efficient transport and the handling of small-sized fern spores; furthermore, this SFS technology provides the basis for fern seedling culture and fern spore industrialization.
Bo Kook Jang; Ju Sung Cho; Cheol Hee Lee. Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores. Plants 2020, 9, 1079 .
AMA StyleBo Kook Jang, Ju Sung Cho, Cheol Hee Lee. Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores. Plants. 2020; 9 (9):1079.
Chicago/Turabian StyleBo Kook Jang; Ju Sung Cho; Cheol Hee Lee. 2020. "Synthetic Seed Technology Development and Production Studies for Storage, Transport, and Industrialization of Bracken Spores." Plants 9, no. 9: 1079.
Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation methods for this species have not been developed. We aimed to develop an efficient propagation method for S. martensii through in vitro culture. We investigated culture conditions that are suitable for shoot-tip proliferation and growth. The optimum shoot-tip culture conditions were determined while using Murashige and Skoog (MS) medium (quarter, half, full, or double strength) and macronutrients (sucrose and two nitrogen sources) at various concentrations. In MS medium, the shoot tips formed a maximum of 6.77 nodes per explant, and each node formed two new shoot tips (i.e., 26 or 64 shoot tips). When using branching segments containing an angle meristem, the shoot-to-rhizophore formation ratio could be controlled by medium supplementation with plant-growth regulators. Sporophytes that were grown from shoot tips in vitro were acclimated in ex vitro soil conditions and successfully survived in the greenhouse. Numerous shoot tips could be obtained from in vitro-grown sporophytes and be proliferated ex vitro to produce a large number of plants. This method provides a way of shortening the time that is required for producing a large stock of S. martensii planting material.
Kyungtae Park; Bo Kook Jang; Ha Min Lee; Ju Sung Cho; Cheol Hee Lee. An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte. Plants 2020, 9, 235 .
AMA StyleKyungtae Park, Bo Kook Jang, Ha Min Lee, Ju Sung Cho, Cheol Hee Lee. An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte. Plants. 2020; 9 (2):235.
Chicago/Turabian StyleKyungtae Park; Bo Kook Jang; Ha Min Lee; Ju Sung Cho; Cheol Hee Lee. 2020. "An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte." Plants 9, no. 2: 235.
Information on the optimal conditions to promote the germination of Lamprocapnos spectabilis (L.) Fukuhara seeds is limited; consequently, this study was conducted to establish the requirements to break their dormancy and promote germination. The selected seeds were morphophysiologically dormant and had not begun embryo development. To study the dormancy breaking and embryo development processes, seeds were subjected to constant or changing temperature treatments during moist stratification. High temperature and humidity conditions resulted in vigorous embryo growth, with the longest embryos occurring after a month of incubation at 20 ℃. At 4 ℃, the seeds required incubation period of at least three months to germinate. Embryo growth and germination were greater with changing high and low temperatures than under a constant temperature, and changing temperatures also considerably changed the endogenous hormone levels, embryo development, and germination. Bioactive gibberellin (GA) content was higher in the seeds incubated at 20 ℃ for one month, then at 4 ℃ for two months. The content of endogenous abscisic acid in the seeds subjected to the same treatment decreased by 97.6 %, compared with that of the untreated seeds. Embryo growth and seed germination require changing high and low temperatures; however, exogenous GA3 could substitute for high temperatures, as it also causes accelerated germination. In this study, the seeds of L. spectabilis were identified as an intermediate simple type, a sub-level of morphophysiologically dormant seeds.
Ju Sung Cho; Bo Kook Jang; Seok Min Lee; In Jung Lee; Cheol Hee Lee. Factors affecting the dormancy and germination of bleeding heart [ Lamprocapnos spectabilis (L.) Fukuhara] seeds. Plant Biology 2020, 22, 514 -521.
AMA StyleJu Sung Cho, Bo Kook Jang, Seok Min Lee, In Jung Lee, Cheol Hee Lee. Factors affecting the dormancy and germination of bleeding heart [ Lamprocapnos spectabilis (L.) Fukuhara] seeds. Plant Biology. 2020; 22 (3):514-521.
Chicago/Turabian StyleJu Sung Cho; Bo Kook Jang; Seok Min Lee; In Jung Lee; Cheol Hee Lee. 2020. "Factors affecting the dormancy and germination of bleeding heart [ Lamprocapnos spectabilis (L.) Fukuhara] seeds." Plant Biology 22, no. 3: 514-521.