A reduction in crop productivity in cultivable land and challenging environmental factors have directed advancement in indoor cultivation systems, such that the yield parameters are higher in outdoor cultivation systems. In wake of this situation, light emitting diode (LED) lighting has proved to be promising in the field of agricultural lighting. Properties such as energy efficiency, long lifetime, photon flux efficacy and flexibility in application make LEDs better suited for future agricultural lighting systems over traditional lighting systems. Different LED spectrums have varied effects on the morphogenesis and photosynthetic responses in plants. LEDs have a profound effect on plant growth and development and also control key physiological processes such as phototropism, the immigration of chloroplasts, day/night period control and the opening/closing of stomata. Moreover, the synthesis of bioactive compounds and antioxidants on exposure to LED spectrum also provides information on the possible regulation of antioxidative defense genes to protect the cells from oxidative damage. Similarly, LEDs are also seen to escalate the nutrient metabolism in plants and flower initiation, thus improving the quality of the crops as well. However, the complete management of the irradiance and wavelength is the key to maximize the economic efficacy of crop production, quality, and the nutrition potential of plants grown in controlled environments. This review aims to summarize the various advancements made in the area of LED technology in agriculture, focusing on key processes such as morphological changes, photosynthetic activity, nutrient metabolism, antioxidant capacity and flowering in plants. Emphasis is also made on the variation in activities of different LED spectra between different plant species. In addition, research gaps and future perspectives are also discussed of this emerging multidisciplinary field of research and its development.

Silicon- (Si-) induced salinity stress resistance was demonstrated at physiological and proteomic levels inCapsicum annuumfor the first time. Seedlings ofC. annuumwere hydroponically treated with NaCl (50 mM) with or without Si (1.8 mM) for 15 days. The results illustrated that saline conditions significantly reduced plant growth and biomass and photosynthetic parameters and increased the electrolyte leakage potential, lipid peroxidation, and hydrogen peroxide level. However, supplementation of Si allowed the plants to recover from salinity stress by improving their physiology and photosynthesis. During salinity stress, Si prevented oxidative damage by increasing the activities of antioxidant enzymes. Furthermore, Si supplementation recovered the nutrient imbalance that had occurred during salinity stress. Additionally, proteomic analysis by two-dimensional gel electrophoresis (2DE) followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) revealed that Si treatment upregulated the accumulation of proteins involved in several metabolic processes, particularly those associated with nucleotide binding and transferase activity. Moreover, Si modulated the expression of vital proteins involved in ubiquitin-mediated nucleosome pathway and carbohydrate metabolism. Overall, the results illustrate that Si application induced resistance against salinity stress inC. annuumby regulating the physiology, antioxidant metabolism, and protein expression.

Tomato plants often grow in saline environments in Mediterranean countries where salt accumulation in the soil is a major abiotic stress that limits its productivity. However, silicon (Si) supplementation has been reported to improve tolerance against several forms of abiotic stress. The primary aim of our study was to investigate, using comparative physiological and proteomic approaches, salinity stress in chloroplasts of tomato under silicon supplementation. Tomato seedlings (Solanum lycopersicum L.) were grown in nutrient media in the presence or absence of NaCl and supplemented with silicon for 5 days. Salinity stress caused oxidative damage, followed by a decrease in silicon concentrations in the leaves of the tomato plants. However, supplementation with silicon had an overall protective effect against this stress. The major physiological parameters measured in our studies including total chlorophyll and carotenoid content were largely decreased under salinity stress, but were recovered in the presence of silicon. Insufficient levels of net-photosynthesis, transpiration and stomatal conductance were also largely improved by silicon supplementation. Proteomics analysis of chloroplasts analyzed by 2D-BN-PAGE (second-dimensional blue native polyacrylamide-gel electrophoresis) revealed a high sensitivity of multiprotein complex proteins (MCPs) such as photosystems I (PSI) and II (PSII) to the presence of saline. A significant reduction in cytochrome b6/f and the ATP-synthase complex was also alleviated by silicon during salinity stress, while the complex forms of light harvesting complex trimers and monomers (LHCs) were rapidly up-regulated. Our results suggest that silicon plays an important role in moderating damage to chloroplasts and their metabolism in saline environments. We therefore hypothesize that tomato plants have a greater capacity for tolerating saline stress through the improvement of photosynthetic metabolism and chloroplast proteome expression after silicon supplementation.

Ammonium sensitivity is considered a globally stressful condition that affects overall crop productivity. The major toxic symptom associated with ammonium nutrition is growth retardation, which has been associated with a high energy cost for maintaining ion, pH, and hormone homeostasis and, eventually, the NH₃/NH₄⁺ level in plant tissues. While certain species/genotypes exhibit extreme sensitivity to ammonium, other species/genotypes prefer ammonium to nitrate as a form of nitrogen. Some of the key tolerance mechanisms used by the plant to deal with NH₄⁺ toxicity include an enhanced activity of an alternative oxidase pathway in mitochondria, greater NH₄⁺ assimilation plus the retention of the minimum level of NH₄⁺ in leaves, and/or poor response to extrinsic acidification or pH drop. Except for toxicity, ammonium can be considered as an energy-efficient nutrition in comparison to nitrate since it is already in a reduced form for use in amino acid metabolism. Through effective manipulation of the NH₄⁺/NO₃ ⁻ ratio, ammonium nutrition can be used to increase productivity, quality, and resistance to various biotic and abiotic stresses of crops. This review highlights recent advancements in ammonium toxicity and tolerance mechanisms, possible strategies to improve ammonium tolerance, and omics-based understanding of nitrogen use efficiency (NUE) in plants.