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Although provisional maximum tolerable daily intake and recommended guidelines have been established for fumonisins (FB) in food, few data are available concerning levels of FB in edible animal tissues. Such data are of particular interest in avian species that can tolerate relatively high levels of fumonisins in their feed. Also, even if multiple contamination of animal feed by toxins produced by Fusarium is very frequent, little is known about the consequences of multiple contamination for FB levels in tissues. The aim of this study was to analyze the concentrations of FB in the muscle and liver of chickens and turkeys fed with FB alone and with FB combined with deoxynivalenol (DON), and with zearalenone (ZEN). Experimental diets were formulated by incorporating ground cultured toxigenic Fusarium strains in corn-soybean based feeds. Control diets were free of mycotoxins, FB diets contained 20 mg FB1+FB2/kg, and FBDONZEN diets contained 20, 5, and 0.5 mg/kg of FB1+FB2, DON, and ZEN, respectively. Animals were reared in individual cages with free access to water and feed. The feed was distributed to male Ross chickens from the 1st to the 35th day of age and to male Grade Maker turkeys from the 55th to the 70th day of age. On the last day of the study, the birds were starved for eight hours, killed, and autopsied for tissues sampling. No sign of toxicity was observed. A UHPLC-MS/MS method with isotopic dilution and immunoaffinity clean-up of samples has been developed for analysis of FB in muscle (n = 8 per diet) and liver (n = 8 per diet). Only traces of FB that were below the LOQ of 0.25 µg/kg were found in most of the samples of animals fed with the control diets. Mean concentrations of FB1, FB2, and FB3 in muscle were 17.5, 3.39, and 1.26 µg/kg, respectively, in chickens, and 5.77, 1.52, and 0.54 µg/kg in turkeys, respectively. In the liver, the respective FB1, FB2, and FB3 concentrations were 44.7, 2.61, and 0.79 µg/kg in chickens, and 41.47, 4.23, and 1.41 µg/kg, in turkeys. Cumulated level of FB1+FB2+FB3 in the highly contaminated samples were above 60 and 100 µg/kg in muscle and liver, respectively. The concentrations of FB in the tissues of animals fed the FBDONZEN diet did not greatly differ from the concentrations measured in animals fed the diet containing only FB.
Didier Tardieu; Angelique Travel; Jean-Paul Metayer; Celeste Le Bourhis; Philippe Guerre. Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level. Toxins 2019, 11, 590 .
AMA StyleDidier Tardieu, Angelique Travel, Jean-Paul Metayer, Celeste Le Bourhis, Philippe Guerre. Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level. Toxins. 2019; 11 (10):590.
Chicago/Turabian StyleDidier Tardieu; Angelique Travel; Jean-Paul Metayer; Celeste Le Bourhis; Philippe Guerre. 2019. "Fumonisin B1, B2 and B3 in Muscle and Liver of Broiler Chickens and Turkey Poults Fed with Diets Containing Fusariotoxins at the EU Maximum Tolerable Level." Toxins 11, no. 10: 590.
Fusarium mycotoxins (FUS) occur frequently in poultry diets, and regulatory limits are laid down in several countries. However, the limits were established for exposure to a single mycotoxin, whereas multiple contamination is more realistic, and different studies have demonstrated that it is not possible to predict interactions between mycotoxins. The purpose of this study was thus to compare the toxic effect of deoxynivalenol (DON), fumonisins (FB) and zearalenone (ZON), alone and in combination on broiler chickens, at the maximum tolerated level established by the EU for poultry feed. Experimental corn-soybean diets incorporated ground cultured toxigenic Fusarium strains. One feed was formulated for chickens 0 to 10 days old and another for chickens 11 to 35 days old. The control diets were mycotoxin free, the DON diets contained 5 mg DON/kg, the FB diet contained 20 mg FB1 + FB2/kg, and the ZON diet contained 0.5mg ZON/kg. The DONFBZON diet contained 5, 20, and 0.5 mg/kg of DON, FB1 + FB2, and ZON, respectively. Diets were distributed ad libitum to 70 broilers (male Ross PM3) separated into five groups of 14 chickens each reared in individual cages from one to 35 days of age. On day 35, after a starvation period of 8 h, a blood sample was collected, and all the animals were killed and autopsied. No difference between groups that could be attributed to FUS was observed in performances, the relative weight of organs, biochemistry, histopathology, intestinal morphometry, variables of oxidative damage, and markers of testicle toxicity. A significant increase in sphinganine and in the sphinganine to sphingosine ratio was observed in broilers fed FB. Taken together, these results suggest that the regulatory guidelines established for single contamination of broiler chickens fed with DON, FB, and ZON can also be used in the case of multiple contamination with these toxins.
Jean-Paul Metayer; Angelique Travel; Amandine Mika; Jean-Denis Bailly; Didier Cleva; Cyril Boissieu; Jean Le Guennec; Pascal Froment; Olivier Albaric; Sophie Labrut; Gurvan Lepivert; Eric Marengue; Didier Tardieu; Philippe Guerre. Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union. Toxins 2019, 11, 455 .
AMA StyleJean-Paul Metayer, Angelique Travel, Amandine Mika, Jean-Denis Bailly, Didier Cleva, Cyril Boissieu, Jean Le Guennec, Pascal Froment, Olivier Albaric, Sophie Labrut, Gurvan Lepivert, Eric Marengue, Didier Tardieu, Philippe Guerre. Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union. Toxins. 2019; 11 (8):455.
Chicago/Turabian StyleJean-Paul Metayer; Angelique Travel; Amandine Mika; Jean-Denis Bailly; Didier Cleva; Cyril Boissieu; Jean Le Guennec; Pascal Froment; Olivier Albaric; Sophie Labrut; Gurvan Lepivert; Eric Marengue; Didier Tardieu; Philippe Guerre. 2019. "Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union." Toxins 11, no. 8: 455.
Céline Repussard; Didier Tardieu; Mélanie Alberich; Philippe Guerre. A new method for the determination of lolitrem B in plant materials. Animal Feed Science and Technology 2014, 193, 141 -147.
AMA StyleCéline Repussard, Didier Tardieu, Mélanie Alberich, Philippe Guerre. A new method for the determination of lolitrem B in plant materials. Animal Feed Science and Technology. 2014; 193 ():141-147.
Chicago/Turabian StyleCéline Repussard; Didier Tardieu; Mélanie Alberich; Philippe Guerre. 2014. "A new method for the determination of lolitrem B in plant materials." Animal Feed Science and Technology 193, no. : 141-147.
Toxicity and persistence of fumonisin B1 (FB1) in liver, kidney and muscle were investigated in ducks fed 5, 10 and 20 mg FB1 + FB2/kg feed during force-feeding. Mortality and signs of toxicity were only obtained with 20 mg/kg, whereas an increased Sa/So ratio was observed from 5 mg/kg on. Persistence of FB1 was only found in liver (16 and 20 μg FB1/kg liver in ducks fed 10 and 20 mg FB1 + FB2/kg feed, respectively). Toxicokinetic studies were conducted by the intravenous route (IV, single dose: 10 mg FB1/kg body weight) and the oral route (single dose: 100 mg FB1/kg body weight), in growing ducks and in ducks during force-feeding. After IV administration, serum concentration–time curves were described by a two-compartment open model. Elimination half-life and mean residence time of FB1 were 26 and 24 min, respectively, clearance was 19.3 ml/min/kg. After oral administration, bioavailability, elimination half-life, mean residence time and clearance varied during force-feeding and growth from 2–2.3%, 71–80 min, 200–188 min, 16.7–17 ml/min/kg, respectively. Taken together these results demonstrate that the risk of persistence of FB1 in ducks after force-feeding is very low, Sa/So being a good biomarker which increases before signs of toxicity and risk of persistence of FB1 in tissue (limit of detection 13 μg/kg).
Didier Tardieu; Jean-Denis Bailly; Imad Benlashehr; Alienor Auby; Jean-Yves Jouglar; Philippe Guerre. Tissue persistence of fumonisin B1 in ducks and after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds. Chemico-Biological Interactions 2009, 182, 239 -244.
AMA StyleDidier Tardieu, Jean-Denis Bailly, Imad Benlashehr, Alienor Auby, Jean-Yves Jouglar, Philippe Guerre. Tissue persistence of fumonisin B1 in ducks and after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds. Chemico-Biological Interactions. 2009; 182 (2-3):239-244.
Chicago/Turabian StyleDidier Tardieu; Jean-Denis Bailly; Imad Benlashehr; Alienor Auby; Jean-Yves Jouglar; Philippe Guerre. 2009. "Tissue persistence of fumonisin B1 in ducks and after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds." Chemico-Biological Interactions 182, no. 2-3: 239-244.
The kinetic of fumonisin B1 (FB1) after a single IV and oral dose, and FB1 persistence in tissue were investigated in turkey poults by HPLC after purification of samples on columns. After IV administration (single-dose: 10 mg FB1/kg bw), serum concentration–time curves were best described by a three-compartment open model. Elimination half-life and mean residence time of FB1 were 85 and 52 min, respectively. After oral administration (single-dose: 100 mg FB1/kg bw) bioavailability was 3.2%; elimination half-life and mean residence time were 214 and 408 min, respectively. Clearance of FB1 was 7.6 and 7.5 ml/min/kg for IV and oral administration respectively. Twenty four hours after the administration of FB1 by the intravenous route, liver and kidney contained the highest levels of FB1 in tissues, level in muscle was low or below the limit of detection (LD, 13 µg/kg). The persistence of FB1 in tissue was also studied after administration for nine weeks of a feed that contained 5, 10 and 20 mg FB1+FB2/kg diet. Eight hours after the last intake of 20 mg FB1+FB2/kg feed (maximum recommended concentration of fumonisins established by the EU for avian feed), hepatic and renal FB1 concentrations were 119 and 22 µg/kg, level in muscles was below the LD
Didier Tardieu; Jean-Denis Bailly; Fabien Skiba; François Grosjean; Philippe Guerre. Toxicokinetics of fumonisin B1 in turkey poults and tissue persistence after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds. Food and Chemical Toxicology 2008, 46, 3213 -3218.
AMA StyleDidier Tardieu, Jean-Denis Bailly, Fabien Skiba, François Grosjean, Philippe Guerre. Toxicokinetics of fumonisin B1 in turkey poults and tissue persistence after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds. Food and Chemical Toxicology. 2008; 46 (9):3213-3218.
Chicago/Turabian StyleDidier Tardieu; Jean-Denis Bailly; Fabien Skiba; François Grosjean; Philippe Guerre. 2008. "Toxicokinetics of fumonisin B1 in turkey poults and tissue persistence after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds." Food and Chemical Toxicology 46, no. 9: 3213-3218.
Immunoaffinity extraction combined with high-performance liquid chromatography (HPLC) with fluorescence detection was developed to determine Fumonisin B1 (FB1) in duck tissues. The method was linear over a concentration range of 0.013–0.250 μg of FB1/g of liver, kidney and muscle. The limit of quantification was 0.013 μg FB1/g in tissue. The mean percentage of extraction was 75% for liver and kidney and 53% for muscle. This method can be used in duck for the detection of FB1 contamination after exposure, the liver being the most contaminated tissue.
Didier Tardieu; Aliénor Auby; Caroline Bluteau; Jean-Denis Bailly; Philippe Guerre. Determination of Fumonisin B1 in animal tissues with immunoaffinity purification. Journal of Chromatography B 2008, 870, 140 -144.
AMA StyleDidier Tardieu, Aliénor Auby, Caroline Bluteau, Jean-Denis Bailly, Philippe Guerre. Determination of Fumonisin B1 in animal tissues with immunoaffinity purification. Journal of Chromatography B. 2008; 870 (1):140-144.
Chicago/Turabian StyleDidier Tardieu; Aliénor Auby; Caroline Bluteau; Jean-Denis Bailly; Philippe Guerre. 2008. "Determination of Fumonisin B1 in animal tissues with immunoaffinity purification." Journal of Chromatography B 870, no. 1: 140-144.