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The aim of the study was to evaluate the main and interactive effects of chromium(III) propionate complex (Cr3) supplementation and different iron supply on the carbohydrate metabolism, lipid profile and other selected biochemical parameters of rats. The experiment was carried out in a two-factor design, in which rats were fed a diet with different proportions of Fe(III) and Cr(III) for six weeks. Fifty-four healthy female Wistar rats were divided into nine experimental groups with different Fe(III) levels, i.e. adequate—control group (45 mg/kg)—100% recommended daily dietary dose of Fe for rodents, deficient (5 mg/kg) and oversupply (180 mg/kg—400%). At the same time they were supplemented with Cr(III) of doses 1 (adequate), 50 and 500 mg/kg of diet. The activity and concentrations of most biochemical parameters were measured with standard enzymatic, kinetic, and colorimetric methods. HOMA-IR and QUICKI indexes were calculated according to appropriate formulas. It was found that there was an interactive effect of high Cr(III) doses and different Fe(III) levels in the diet on the carbohydrate metabolism and insulin resistance indexes. The presented results suggested that iron deficient diet fed animals led to insulin resistance; however, an effect is attenuated by Cr(III) supplementation at high doses. There were no significant changes in the rats’ lipid profile (except for the high density lipoprotein cholesterol (HDL-C) level) and most of the other biochemical parameters, such as the leptin, aspartate aminotransferase (AST), alanine transaminase (ALT), total protein (TP), creatinine (Crea) and the urea (BUN) concentrations. The study proved that the Cr(III) supplementation, independently and in combination with diversified Fe(III) content in the diet, affected the carbohydrate metabolism and insulin resistance indexes but did not affect lipid profile and most of the other biochemical parameters in healthy rats. The findings proved the role of Fe and Cr(III) and their interactions on disturbances carbohydrates metabolism.
Halina Zofia Staniek; Ewelina Król; Rafał Wojciech Wójciak. The Interactive Effect of High Doses of Chromium(III) and Different Iron(III) Levels on the Carbohydrate Status, Lipid Profile, and Selected Biochemical Parameters in Female Wistar Rats. Nutrients 2020, 12, 3070 .
AMA StyleHalina Zofia Staniek, Ewelina Król, Rafał Wojciech Wójciak. The Interactive Effect of High Doses of Chromium(III) and Different Iron(III) Levels on the Carbohydrate Status, Lipid Profile, and Selected Biochemical Parameters in Female Wistar Rats. Nutrients. 2020; 12 (10):3070.
Chicago/Turabian StyleHalina Zofia Staniek; Ewelina Król; Rafał Wojciech Wójciak. 2020. "The Interactive Effect of High Doses of Chromium(III) and Different Iron(III) Levels on the Carbohydrate Status, Lipid Profile, and Selected Biochemical Parameters in Female Wistar Rats." Nutrients 12, no. 10: 3070.
This study evaluated the effect of adding a new step, termed conditioning, to the traditional processing of leaves from Morus alba var. zolwinska wielkolistna grown in Poland (WML-P). This step, modeled on tea leaves processing, was conducted in a controlled environment on a semi-technical scale. The primary goal was to evaluate the effect of the WML-P conditioning for 1–4 h at 32–35 °C on the content of bioactive compounds (total phenolics, phenolic acids, flavonols, 1-deoxynojirimycin) and antioxidant activity (radical scavenging against DPPH, antioxidant capacity, chelating activity and ferric reducing antioxidant potential) of the lyophilized extracts. For the first time WML-P extracts content was comprehensively characterized by assessing dietary fiber fractions, fatty acids, amino acids, macro- and microelements and chlorophyll content. Compared to the traditional process, adding the conditioning step to WML-P processing resulted in an increased total phenolics content, radical scavenging capacity, ability to quench 2,2-diphenyl-1-picrylhydrazyl (DPPH·) and iron-chelating ability in the lyophilized extracts. The beneficial effect depended on conditioning time. The highest flavonols and phenolic acids content were found after 2-h conditioning. We concluded that adding a 2-h conditioning step to traditional WML-P processing results in getting WML-P lyophilized extract with increased bioactive compounds content and high antioxidant activity.
Monika Przeor; Ewa Flaczyk; Dominik Kmiecik; Maciej S. Buchowski; Halina Staniek; Aneta Tomczak-Graczyk; Joanna Kobus-Cisowska; Anna Gramza-Michałowska; Joanna Foksowicz-Flaczyk. Functional Properties and Antioxidant Activity of Morus alba L. Leaves var. Zolwinska Wielkolistna (WML-P)—The Effect of Controlled Conditioning Process. Antioxidants 2020, 9, 668 .
AMA StyleMonika Przeor, Ewa Flaczyk, Dominik Kmiecik, Maciej S. Buchowski, Halina Staniek, Aneta Tomczak-Graczyk, Joanna Kobus-Cisowska, Anna Gramza-Michałowska, Joanna Foksowicz-Flaczyk. Functional Properties and Antioxidant Activity of Morus alba L. Leaves var. Zolwinska Wielkolistna (WML-P)—The Effect of Controlled Conditioning Process. Antioxidants. 2020; 9 (8):668.
Chicago/Turabian StyleMonika Przeor; Ewa Flaczyk; Dominik Kmiecik; Maciej S. Buchowski; Halina Staniek; Aneta Tomczak-Graczyk; Joanna Kobus-Cisowska; Anna Gramza-Michałowska; Joanna Foksowicz-Flaczyk. 2020. "Functional Properties and Antioxidant Activity of Morus alba L. Leaves var. Zolwinska Wielkolistna (WML-P)—The Effect of Controlled Conditioning Process." Antioxidants 9, no. 8: 668.
Trivalent chromium (Cr) and bitter melon (Momordica charantia L., BM) have been shown to independently interact with the insulin signaling pathway leading to improvements in the symptoms of insulin resistance and diabetes in some animal models and human subjects. The aim of this study was to examine whether the combination of the two nutritional supplements could potentially have additive effects on treating these conditions in high-fat-fed streptozotocin (STZ)-induced diabetic rats. The experiment was conducted with 110 male Wistar rats divided into eleven groups and fed either a control or high-fat diet for 7 weeks. Half of the rats on the high-fat diet were injected with STZ (30 mg/kg body mass) to induce diabetes. The high-fat (HF) diets were then supplemented with a combination of Cr (as chromium(III) propionate complex, Cr3: either 10 or 50 mg Cr/kg diet) and bitter melon (lyophilized whole fruit: either 10 or 50 g/kg diet) for 6 weeks. After termination of the experiment, blood and internal organs were harvested for blood biochemical, hematological, and mineral (Cr) analyses using appropriate analytical methods. It was found that neither Cr(III) nor BM was able to significantly affect blood indices in HF and diabetic rats, but BM tended to improve body mass gain, blood glucose, and LDL cholesterol values, but decreased Cr content in the liver and kidneys of the Cr-co-supplemented type 2 diabetic model of rats. Supplementary Cr(III) had no appreciable effect on glucose and lipid metabolism in high-fat-fed STZ-induced diabetic rats. Supplementary BM fruit powder had some observable effects on body mass of high-fat-fed rats; these effects seem to be dampened when BM was co-administered with Cr. Cr(III) and BM appear to act as nutritional antagonists when both administered in food, probably due to binding of Cr by the polyphenol-type compounds present in the plant material.
Pandora E. White; Ewelina Król; Artur Szwengiel; Małgorzata Tubacka; Dawid Szczepankiewicz; Halina Staniek; John B. Vincent; Zbigniew Krejpcio. Effects of Bitter Melon and a Chromium Propionate Complex on Symptoms of Insulin Resistance and Type 2 Diabetes in Rat Models. Biological Trace Element Research 2020, 199, 1013 -1026.
AMA StylePandora E. White, Ewelina Król, Artur Szwengiel, Małgorzata Tubacka, Dawid Szczepankiewicz, Halina Staniek, John B. Vincent, Zbigniew Krejpcio. Effects of Bitter Melon and a Chromium Propionate Complex on Symptoms of Insulin Resistance and Type 2 Diabetes in Rat Models. Biological Trace Element Research. 2020; 199 (3):1013-1026.
Chicago/Turabian StylePandora E. White; Ewelina Król; Artur Szwengiel; Małgorzata Tubacka; Dawid Szczepankiewicz; Halina Staniek; John B. Vincent; Zbigniew Krejpcio. 2020. "Effects of Bitter Melon and a Chromium Propionate Complex on Symptoms of Insulin Resistance and Type 2 Diabetes in Rat Models." Biological Trace Element Research 199, no. 3: 1013-1026.
This study compared the mineral content and bioactive properties of flowers and fruit coming from wild elderberry plants with those of flowers and fruit harvested from elderberry cultivars grown in an orchard. Elderberry fruit and flowers were analyzed for the content of selected minerals, phenolic compounds, and anthocyanins, as well as for antioxidant activity. Mineral content was determined by the atomic absorption spectrometry method, while antioxidant activity and the content of polyphenols and anthocyanins were determined by spectrophotometric methods. Flowers were found to contain more total ash and much higher content of most of minerals, except magnesium which was present in high concentrations in fruit. Fruit showed significantly higher antioxidant activity than flowers, whereas the total phenolic content varied depending on the growing location or cultivar. The material obtained from selected cultivars growing in an orchard had higher antioxidant activity and polyphenol and anthocyanin content than the material obtained from wild plants. Fruit of the ”Haschberg” cultivar and flowers of the ”Sampo” cultivar had the best bioactive properties of the studied samples.
Karolina Młynarczyk; Dorota Walkowiak-Tomczak; Halina Staniek; Marcin Kidoń; Grzegorz P. Łysiak. The Content of Selected Minerals, Bioactive Compounds, and the Antioxidant Properties of the Flowers and Fruit of Selected Cultivars and Wildly Growing Plants of Sambucus nigra L. Molecules 2020, 25, 876 .
AMA StyleKarolina Młynarczyk, Dorota Walkowiak-Tomczak, Halina Staniek, Marcin Kidoń, Grzegorz P. Łysiak. The Content of Selected Minerals, Bioactive Compounds, and the Antioxidant Properties of the Flowers and Fruit of Selected Cultivars and Wildly Growing Plants of Sambucus nigra L. Molecules. 2020; 25 (4):876.
Chicago/Turabian StyleKarolina Młynarczyk; Dorota Walkowiak-Tomczak; Halina Staniek; Marcin Kidoń; Grzegorz P. Łysiak. 2020. "The Content of Selected Minerals, Bioactive Compounds, and the Antioxidant Properties of the Flowers and Fruit of Selected Cultivars and Wildly Growing Plants of Sambucus nigra L." Molecules 25, no. 4: 876.
Marcin Dziedziński; Ewelina Król; Halina Staniek; Oskar Szczepaniak; Joanna Kobus-Cisowska; Aleksandra Telichowska; Ewa Flaczyk; Szymon Byczkiewicz. Effect of metal ions from Ginkgo biloba extracts on the oxidative stability of rapeseed oil and its triacylglycerols. Journal of Elementology 2019, 1 .
AMA StyleMarcin Dziedziński, Ewelina Król, Halina Staniek, Oskar Szczepaniak, Joanna Kobus-Cisowska, Aleksandra Telichowska, Ewa Flaczyk, Szymon Byczkiewicz. Effect of metal ions from Ginkgo biloba extracts on the oxidative stability of rapeseed oil and its triacylglycerols. Journal of Elementology. 2019; (3/2020):1.
Chicago/Turabian StyleMarcin Dziedziński; Ewelina Król; Halina Staniek; Oskar Szczepaniak; Joanna Kobus-Cisowska; Aleksandra Telichowska; Ewa Flaczyk; Szymon Byczkiewicz. 2019. "Effect of metal ions from Ginkgo biloba extracts on the oxidative stability of rapeseed oil and its triacylglycerols." Journal of Elementology , no. 3/2020: 1.
It is suggested that both iron overload and chromium(III) deficiency may be risk factors of diabetes. It seems that both Fe and Cr(III) metabolism as well as copper and zinc metabolism are interrelated. However, the direction of these changes may depend on mutual proportions of these elements in the diet and organism. The aim of the study was to evaluate the combined effects of Cr(III) supplementation with Fe excess on the Cu and Zn status in female rats. Thirty-six healthy rats were divided into 6 experimental groups with different Fe levels in the diet. Groups marked with C (control) contained Fe at the recommended level (45 mg kg-1). The excess groups (E) contained Fe at 180 mg kg-1. At the same time the animals were supplemented with Cr(III) of doses 1, 50 and 500 mg kg-1 of diet. The Cr, Fe, Cu and Zn dietary and tissular contents were measured with the AAS method.The excess Fe in the diet significantly decreased the Cu content in the liver and kidneys, but it increased the spleen Cu level. The Cr(III) supplementary did not affect the tissular Cu levels, regardless of Fe supply with diet. The experimental factors did not have significant interactional effect on the Cu status parameters under study.The Fe excess in the diet reduced the renal and splenic Zn content, but increased the heart Zn content. The Cr(III) supplementation decreased the Zn content in the kidneys. The Zn content in the liver and spleen tended to decrease as the Cr(III) supply in the diet increased. There was no significant interactional effect of Cr(III) supplementation and the Fe excessive supply in diet on the parameters of Zn metabolism in Wistar rats. Iron oversupply disturbed the rat's Cu and Zn status. However, Cr(III) supplementation did not affect the tissular levels of these elements, except the kidney Zn content. Simultaneous supplementation with the Cr(III) propionate complex did not deepen changes in tissular Cu and Zn levels caused by the Fe excess in the diet.
Halina Staniek. The combined effects of Cr(III) propionate complex supplementation and iron excess on copper and zinc status in rats. Journal of Trace Elements in Medicine and Biology 2019, 53, 49 -54.
AMA StyleHalina Staniek. The combined effects of Cr(III) propionate complex supplementation and iron excess on copper and zinc status in rats. Journal of Trace Elements in Medicine and Biology. 2019; 53 ():49-54.
Chicago/Turabian StyleHalina Staniek. 2019. "The combined effects of Cr(III) propionate complex supplementation and iron excess on copper and zinc status in rats." Journal of Trace Elements in Medicine and Biology 53, no. : 49-54.
The aim of the study was to assess the combined effects of chromium(III) supplementation and iron deficiency on the copper (Cu) and zinc (Zn) status in female rats. The Cr, Fe, Cu and Zn dietary and tissular levels were measured by Atomic Absorption Spectrometry (AAS) method. The data show that chromium(III) supplementation compensated for the negative effects of Fe deficiency on the Cu content but it deepened the effect on Zn levels in the female rats. Detailed data on the status of trace elements and their interactions in healthy subjects and patients with metabolic disorders (e.g. anaemia, diabetes mellitus) are strongly required for effective nutritional and therapeutic strategies.
Halina Staniek. The Combined Effects of Cr(III) Supplementation and Iron Deficiency on the Copper and Zinc Status in Wistar Rats. Biological Trace Element Research 2018, 190, 414 -424.
AMA StyleHalina Staniek. The Combined Effects of Cr(III) Supplementation and Iron Deficiency on the Copper and Zinc Status in Wistar Rats. Biological Trace Element Research. 2018; 190 (2):414-424.
Chicago/Turabian StyleHalina Staniek. 2018. "The Combined Effects of Cr(III) Supplementation and Iron Deficiency on the Copper and Zinc Status in Wistar Rats." Biological Trace Element Research 190, no. 2: 414-424.
Halina Staniek; Zbigniew Krejpcio; Rafal W. Wojciak; Katarzyna Prokop; Małgorzata Tubacka. Combined effect of diversified Fe(III) content in the diet and Cr(III) supplementation on the magnesium status in rats. Journal of Elementology 2018, 1 .
AMA StyleHalina Staniek, Zbigniew Krejpcio, Rafal W. Wojciak, Katarzyna Prokop, Małgorzata Tubacka. Combined effect of diversified Fe(III) content in the diet and Cr(III) supplementation on the magnesium status in rats. Journal of Elementology. 2018; (2/2018):1.
Chicago/Turabian StyleHalina Staniek; Zbigniew Krejpcio; Rafal W. Wojciak; Katarzyna Prokop; Małgorzata Tubacka. 2018. "Combined effect of diversified Fe(III) content in the diet and Cr(III) supplementation on the magnesium status in rats." Journal of Elementology , no. 2/2018: 1.
The aim of the study was to evaluate the combined effect of supplementary chromium(III) and iron deficiency on the chromium and iron status in female rats. The study was carried out on female Wistar rats, which were divided into 6 experimental groups with different Fe levels (deficient 10% RDA and recommended (adequate) 100% RDA). Simultaneously, for six weeks their diets were supplemented with Cr(III) at doses of 1, 50 and 500mgkg-1. The tissular chromium and iron levels were measured with the AAS method. The serum iron and TIBC were measured with colorimetric methods The serum ferritin level was measured by means of electrochemiluminescence immunoassay. The serum transferrin level was measured with the ELISA method. The haematology was measured with an automated blood analyser. Supplementary Cr3 increased the Cr content in the tissues. This effect was weaker in the Fe-deficient groups than in those with the recommended Fe level, but it did not affect the Fe status. Fe deficiency significantly reduced the Fe content in the tissues. Simultaneously, Cr3 supplementation mitigated the symptoms of Fe deficiency. Fe deficiency increased TIBC and transferrin levels but reduced ferritin and most haematological parameters. However, simultaneous addition of high doses of Cr3 did not deepen these adverse changes. Our results show that the trend of changes in the Fe-Cr interaction depends on the content of these elements in the body.
Halina Staniek; Rafał W. Wójciak. The combined effect of supplementary Cr(III) propionate complex and iron deficiency on the chromium and iron status in female rats. Journal of Trace Elements in Medicine and Biology 2018, 45, 142 -149.
AMA StyleHalina Staniek, Rafał W. Wójciak. The combined effect of supplementary Cr(III) propionate complex and iron deficiency on the chromium and iron status in female rats. Journal of Trace Elements in Medicine and Biology. 2018; 45 ():142-149.
Chicago/Turabian StyleHalina Staniek; Rafał W. Wójciak. 2018. "The combined effect of supplementary Cr(III) propionate complex and iron deficiency on the chromium and iron status in female rats." Journal of Trace Elements in Medicine and Biology 45, no. : 142-149.
Inadequate iron supply has significant consequences to health. There are some relations between the metabolism of different trace elements, such as iron, zinc, copper and chromium. However, the direction of these interactions can be antagonistic or synergistic, and it depends on many factors. The aim of the study was to evaluate the combined effects of supplementary of chromium(III) propionate complex (Cr3) with iron excess on the Cr and Fe status in healthy female rats. The 36 healthy female Wistar rats were divided into six experimental groups (six animals in each) with different Fe levels—adequate (45 mg kg−1—100% RDA) and high (excessive—180 mg kg−1—400% RDA). At the same time, they were supplemented with Cr(III) at doses of 1, 50 and 500 mg kg−1 of diet: C1—control (Fe 45 mg kg−1, Cr 1 mg kg−1); C50 (Fe 45 mg kg−1, Cr 50 mg kg−1); C500 (Fe 45 mg kg−1, Cr 500 mg kg−1); H1 (Fe 180 mg kg−1, Cr 1 mg kg−1); H50 (Fe 180 mg kg−1, Cr 50 mg kg−1); H500 (Fe 180 mg kg−1, Cr 500 mg kg−1). The serum iron level and total iron binding capacity (TIBC) were measured with colorimetric methods. The serum ferritin level was measured by means of electrochemiluminescence immunoassay. The serum transferrin level was measured with the ELISA method. Haematological measurements were made with an automated blood analyser. The Cr and Fe tissular levels were measured with the AAS method. The exposure to a high level of Fe(III) alone or in combination with Cr caused Fe accumulation in tissues, especially in the liver and kidneys, but there were no significant changes in the TIBC, transferrin, ferritin concentration in the serum and most haematological parameters. Moreover, the serum, hepatic and renal Cr concentrations decreased. The doses of supplementary Cr(III) given separately or in combination with high level of Fe(III) disturbed the Cr content in the liver and kidneys of healthy female rats. However, they did not change most of the parameters of Fe metabolism, except the Fe kidney concentration. Supplementary Cr3 decreased the renal Fe level in groups with adequate Fe content in the diet. However, the renal Fe levels increased along with a higher Cr level in the diet in groups with high Fe content. The findings proved a relationship between Fe(III) and Cr(III) metabolism in healthy female rats. However, the direction of change varied and depended on relative amounts of these elements in the diet.
Halina Staniek; Rafal W. Wojciak. The Combined Effects of Iron Excess in the Diet and Chromium(III) Supplementation on the Iron and Chromium Status in Female Rats. Biological Trace Element Research 2017, 184, 398 -408.
AMA StyleHalina Staniek, Rafal W. Wojciak. The Combined Effects of Iron Excess in the Diet and Chromium(III) Supplementation on the Iron and Chromium Status in Female Rats. Biological Trace Element Research. 2017; 184 (2):398-408.
Chicago/Turabian StyleHalina Staniek; Rafal W. Wojciak. 2017. "The Combined Effects of Iron Excess in the Diet and Chromium(III) Supplementation on the Iron and Chromium Status in Female Rats." Biological Trace Element Research 184, no. 2: 398-408.
More and more people use food supplements for various reasons, e.g. to prevent mineral deficiency and diseases (e.g. osteoporosis, diabetes, anaemia). Supplements containing Cr(III) are purchased primarily for weight loss and antidiabetic effects. The aim of this study was to evaluate the effects of supplementary Cr3 {chromium(III) propionate complex, [Cr3O(O2CCH2CH3)6(H2O)3]NO3)} on the mineral status in female Wistar rats. The study was carried out on 30 female Wistar rats, divided into five groups (six animals in each): a control group and test groups fed Cr3 supplemented diets with 100, 200, 500 and 1000 mg Cr · kg-1 diet (equivalent to 10, 20, 50 and 100 mg Cr ·kg-1 body mass (b.m.) per day) given as Cr3 for 4 weeks. Supplementary Cr3 increased the Cr content in tissues in a dose-dependent manner. High dietary doses of Cr3, 20 and 100 mg Cr · kg-1 b.m., increased the Cu content in the liver and spleen as well as the Zn content in the kidneys but decreased the liver Ca content. Doses of 50-100 mg Cr ·kg-1 b.m. decreased the serum Fe concentration and the Fe content in the liver and kidneys. Supplementation with Cr3 at doses of 10 and 100 mg Cr ·kg-1 b.m. did not affect the Mg content in the rats' tissues. In conclusion, high dietary doses of Cr3 (10 and 100 mg Cr· kg-1 b.m.) given for 4 weeks affected the mineral status of Fe, Zn, Cu and Ca in the tissues of healthy female Wistar rats.
Halina Staniek; Zbigniew Krejpcio. The Effects of Supplementary Cr3 (Chromium(III) Propionate Complex) on the Mineral Status in Healthy Female Rats. Biological Trace Element Research 2017, 180, 90 -99.
AMA StyleHalina Staniek, Zbigniew Krejpcio. The Effects of Supplementary Cr3 (Chromium(III) Propionate Complex) on the Mineral Status in Healthy Female Rats. Biological Trace Element Research. 2017; 180 (1):90-99.
Chicago/Turabian StyleHalina Staniek; Zbigniew Krejpcio. 2017. "The Effects of Supplementary Cr3 (Chromium(III) Propionate Complex) on the Mineral Status in Healthy Female Rats." Biological Trace Element Research 180, no. 1: 90-99.
Product may be a valuable source of iron in the nutrition of individuals with a deficiency of this element, first of all patients with absorption and metabolism disorders, but also may add variety to the traditional daily diet.
Julita Regula; Zbigniew Krejpcio; Halina Staniek. Iron bioavailability from cereal products enriched with Pleurotus ostreatus mushrooms in rats with induced anaemia. Annals of Agricultural and Environmental Medicine 2016, 23, 310 -314.
AMA StyleJulita Regula, Zbigniew Krejpcio, Halina Staniek. Iron bioavailability from cereal products enriched with Pleurotus ostreatus mushrooms in rats with induced anaemia. Annals of Agricultural and Environmental Medicine. 2016; 23 (2):310-314.
Chicago/Turabian StyleJulita Regula; Zbigniew Krejpcio; Halina Staniek. 2016. "Iron bioavailability from cereal products enriched with Pleurotus ostreatus mushrooms in rats with induced anaemia." Annals of Agricultural and Environmental Medicine 23, no. 2: 310-314.
People taking dietary supplements are usually determined to lose weight, supplement nutrition or reduce the risk of illness and negative effects of their state of health. Chromium(III) supplementation influence body composition and mass, glucose and lipid metabolism and it enhance insulin action. This fact could be of general interest because diabetes mellitus is an increasing health problem in many countries. The study describes the effects of high dietary doses of chromium(III) complex with propionic acid [Cr3] (from 100 to 1000 mg Cr · kg−1 diet) on the organisms of healthy female rats, with special regard to overall nutritional, carbohydrate, lipid and blood biochemical and morphological and haematological indices. The study was carried out on 30 10-week-old female Wistar rats, which were divided into five equal groups (six animals in each): the control group and four groups of tested animals which had free access to the diet supplemented with 100, 200, 500 and 1000 mg Cr · kg−1 (equivalent of 10, 20, 50 and 100 mg Cr · kg body weight (b.w.) · day-1), given as [Cr3O(O2CCH2CH3)6(H2O)3]⋅NO3, also known as Cr3, for 4 weeks. There were no significant differences in body mass gains, feeding efficiency ratio, internal organ masses or blood serum glucose concentrations, except for some changes in the serum triglycerides concentration, which decreased in the rats that received 500 and 1000 mg Cr · kg−1 diet, as opposed to the group treated with 200 mg Cr · kg−1 diet. The dietary supplementation of Cr3 for 4 weeks at doses of 100 to 1000 mg Cr · kg−1 diet did not affect overall nutritional indices and most blood biochemical, morphological and haematological indices.
Halina Staniek; Zbigniew Krejpcio; Daria Wieczorek. The Effects of High Dietary Doses of Chromium(III) Complex with Propionic Acid on Nutritional and Selected Blood Indices in Healthy Female Rats. Biological Trace Element Research 2015, 171, 192 -200.
AMA StyleHalina Staniek, Zbigniew Krejpcio, Daria Wieczorek. The Effects of High Dietary Doses of Chromium(III) Complex with Propionic Acid on Nutritional and Selected Blood Indices in Healthy Female Rats. Biological Trace Element Research. 2015; 171 (1):192-200.
Chicago/Turabian StyleHalina Staniek; Zbigniew Krejpcio; Daria Wieczorek. 2015. "The Effects of High Dietary Doses of Chromium(III) Complex with Propionic Acid on Nutritional and Selected Blood Indices in Healthy Female Rats." Biological Trace Element Research 171, no. 1: 192-200.
The smallest tolerance was noted for lentil seeds and wheat grains. Other plants developed in 25 mM solution of FeSO4. The highest accumulation of iron was observed in alfalfa sprouts. However, lupine and soybean seeds are the most recommended raw material for the production of the sprouts on an industrial scale.
Magdalena Zielińska-Dawidziak; Halina Staniek; Ewelina Król; Dorota Piasecka-Kwiatkowska; Tomasz Twardowski. Legume seeds and cereal grains’ capacity to accumulate iron while sprouting in order to obtain food fortifi cant. Acta Scientiarum Polonorum Technologia Alimentaria 2015, 15, 333 -338.
AMA StyleMagdalena Zielińska-Dawidziak, Halina Staniek, Ewelina Król, Dorota Piasecka-Kwiatkowska, Tomasz Twardowski. Legume seeds and cereal grains’ capacity to accumulate iron while sprouting in order to obtain food fortifi cant. Acta Scientiarum Polonorum Technologia Alimentaria. 2015; 15 (3):333-338.
Chicago/Turabian StyleMagdalena Zielińska-Dawidziak; Halina Staniek; Ewelina Król; Dorota Piasecka-Kwiatkowska; Tomasz Twardowski. 2015. "Legume seeds and cereal grains’ capacity to accumulate iron while sprouting in order to obtain food fortifi cant." Acta Scientiarum Polonorum Technologia Alimentaria 15, no. 3: 333-338.
The aim of this work was to develop a CdSe/ZnS quantum dot ‐based fluorescence method for the determination of quercetin aglycone in onion. The synthesized nanostructures have been characterized by the use of spectroscopic techniques. The method developed is based on the formation of host–guest complexes between quercetin aglycone and β‐cyclodextrin and the consequent fluorescence quenching of the quantum dots. This allowed us to obtain a fast and selective spectroscopic method for the determination of this flavonoid. The developed technique enables determination of quercetin aglycone in real samples (methanolic extracts obtained from onion) in the presence of all components of the matrix, including quercetin glucosides and other phenolic compounds. The limit of detection was 14 μm and sensitivity was 13.2 nm. The accuracy of the method has been confirmed by HPLC measurements and the average recovery was in the 108–113% range, depending on the kind of sample tested.
Krzysztof Dwiecki; Paulina Kwiatkowska; Aleksander Siger; Halina Staniek; Malgorzata Nogala-Kalucka; Krzysztof Polewski. Determination of quercetin in onion (Allium cepa) using β-cyclodextrin-coated CdSe/ZnS quantum dot-based fluorescence spectroscopic technique. International Journal of Food Science & Technology 2015, 50, 1366 -1373.
AMA StyleKrzysztof Dwiecki, Paulina Kwiatkowska, Aleksander Siger, Halina Staniek, Malgorzata Nogala-Kalucka, Krzysztof Polewski. Determination of quercetin in onion (Allium cepa) using β-cyclodextrin-coated CdSe/ZnS quantum dot-based fluorescence spectroscopic technique. International Journal of Food Science & Technology. 2015; 50 (6):1366-1373.
Chicago/Turabian StyleKrzysztof Dwiecki; Paulina Kwiatkowska; Aleksander Siger; Halina Staniek; Malgorzata Nogala-Kalucka; Krzysztof Polewski. 2015. "Determination of quercetin in onion (Allium cepa) using β-cyclodextrin-coated CdSe/ZnS quantum dot-based fluorescence spectroscopic technique." International Journal of Food Science & Technology 50, no. 6: 1366-1373.
An isolate of lead-ferritin obtained from soybean seeds sprouted in 25 mM of PbNO3 was introduced into the diet of both iron-deficient and iron non-deficient male rats. After a 21-day administration period, statistical differences in the lead accumulation in the femurs of the rats were noted. Iron-deficient rats accumulated more than four times the amount of lead in their bones than rats without iron-deficiency. No further decrease was observed in haemoglobin concentrations in the groups of animals fed with lead isolates, either iron-deficient or iron non-deficient. Also, no differences in the mean corpuscular haemoglobin (MCH) and mean corpuscular volume (MCV) were observed at the end of the experiment in the group of iron non-deficient rats fed with lead-ferritin isolate compared to the control group of iron non-deficient rats. In the iron-deficient group fed with lead-ferritin isolate, a small increase in haemoglobin concentrations, MCH, MCV and mean corpuscular haemoglobin concentrations (MCHC) was recorded. The results presented in this paper confirm that lead from the tested preparation-lead ferritin isolate-was better absorbed by those rats with induced iron deficiency anaemia. Additionally, we may also suspect based on the obtained results that absorption of ferritin-iron depends on iron status in the body.
Magdalena Zielińska-Dawidziak; Iwona Hertig; Halina Staniek; Dorota Piasecka-Kwiatkowska; Krzysztof W. Nowak. Effect of iron status in rats on the absorption of metal ions from plant ferritin. Plant Foods for Human Nutrition 2014, 69, 101 -7.
AMA StyleMagdalena Zielińska-Dawidziak, Iwona Hertig, Halina Staniek, Dorota Piasecka-Kwiatkowska, Krzysztof W. Nowak. Effect of iron status in rats on the absorption of metal ions from plant ferritin. Plant Foods for Human Nutrition. 2014; 69 (2):101-7.
Chicago/Turabian StyleMagdalena Zielińska-Dawidziak; Iwona Hertig; Halina Staniek; Dorota Piasecka-Kwiatkowska; Krzysztof W. Nowak. 2014. "Effect of iron status in rats on the absorption of metal ions from plant ferritin." Plant Foods for Human Nutrition 69, no. 2: 101-7.
The long-term use of hypotensive drugs may cause side effects, including impaired glucose metabolism and mineral status. This study tested the hypothesis that some hypotensive drugs can affect tissular chromium levels and indices of glucose metabolic and antioxidant potential in rats. The experiment was performed on 40 male spontaneously hypertensive rats (SHRs), which were assigned to five groups: control (C), with perindopril (PR), with metoprolol (MT), with indapamide (ID), and with amlodipine (AM). All rats were provided ad libitum standard diet (with or without drugs) and distilled water for 45 days. Glucose and insulin levels, along with total antioxidant status (TAS) and concentrations of TNF-alpha and C-reactive protein, were assayed in serum. Chromium concentrations in the liver and kidney were determined using the flame atomic absorption spectrometry method. Detailed statistical analysis was performed using Statistica for Windows 10.0 (StatSoft, Poland). One-way analysis of variance (ANOVA), followed by a post hoc Tukey test, was used to compare the data between groups. Treatment with indapamide and amlodipine resulted in significantly higher chromium concentrations in the liver and kidney (AM) of the rats, compared with the control group. A markedly higher concentration of glucose was found in the ID group. Treatment with amlodipine significantly increased TAS levels in serum and decreased TNF-alpha concentration in serum of the rats. A significant positive correlation between chromium concentration in tissues and serum TAS level was observed, as was a significant negative correlation between chromium concentration in the kidneys, and TNF-alpha and glucose levels in serum. In conclusion, the administration of amlodipine may lead to an increase in chromium accumulation in the internal organs, which is associated with increased antioxidant status and suppression of the inflammatory response of cells in SHRs.
Joanna Suliburska; Zbigniew Krejpcio; Halina Staniek; Ewelina Król; Pawel Bogdanski; Justyna Kupsz; Iwona Hertig. The Effects of Antihypertensive Drugs on Chromium Status, Glucose Metabolism, and Antioxidant and Inflammatory Indices in Spontaneously Hypertensive Rats. Biological Trace Element Research 2013, 157, 60 -66.
AMA StyleJoanna Suliburska, Zbigniew Krejpcio, Halina Staniek, Ewelina Król, Pawel Bogdanski, Justyna Kupsz, Iwona Hertig. The Effects of Antihypertensive Drugs on Chromium Status, Glucose Metabolism, and Antioxidant and Inflammatory Indices in Spontaneously Hypertensive Rats. Biological Trace Element Research. 2013; 157 (1):60-66.
Chicago/Turabian StyleJoanna Suliburska; Zbigniew Krejpcio; Halina Staniek; Ewelina Król; Pawel Bogdanski; Justyna Kupsz; Iwona Hertig. 2013. "The Effects of Antihypertensive Drugs on Chromium Status, Glucose Metabolism, and Antioxidant and Inflammatory Indices in Spontaneously Hypertensive Rats." Biological Trace Element Research 157, no. 1: 60-66.
Diabetes results in several metabolic changes, including alterations in the transport, distribution, excretion, and accumulation of metals. While changes have been examined in several rat models of insulin resistance and diabetes, the metal ion concentrations in the tissues of Zucker lean, Zucker obese (an insulin resistance and early stage diabetes model), and Zucker diabetic fatty (ZDF, a type 2 diabetes model) have not previously been examined in detail. The concentration of Cu, Zn, Fe, Mg, and Ca were examined in the liver, kidney, heart and spleen, and Cr concentration in the liver and kidney of these rats were examined. Zucker obese rats have a reduction in the concentration of Cu, Zn, Fe, Mg in the liver compared to ZDF and/or lean Zucker rats, presumably as a result of the increased fat content of the liver of the obese rats. ZDF rats have increased concentrations of kidney Cu compared to the lean rats, while kidney Ca concentrations are increased in the Zucker obese rats. Spleen Fe concentrations are decreased in Zucker obese rats compared to the lean rats. No effects on metal concentrations in the heart were observed between the lean, obese, and ZDF rats, and no effects on Cr concentrations were identified. Cr(III) complexes have previously been shown to have beneficial effects on the signs of insulin resistance in Zucker obese and ZDF rats. The effects of daily gavage administration of chromium picolinate ([Cr(pic)3]) (1 mg Cr/kg body mass), CrCl3 (1 mg Cr/kg body mass), and Cr3 ([Cr3O(propionate)6(H2O)3]+) (33 μg and 1 mg Cr/kg body mass) on metal concentrations in these tissues were examined. Treatment with CrCl3 and Cr3, but not [Cr(pic)3], at 1 mg Cr/kg resulted in a statistically significant accumulation of Cr in the kidney of lean and obese but not ZDF rats but resulted in lowering the elevated levels of kidney Cu in ZDF rats, suggesting a beneficial effect on this symptom of type 2 diabetes.
Halina Staniek; Nicholas R. Rhodes; Kristin Di Bona; Ge Deng; Sharifa T. Love; Leigh Ann Pledger; Jeremy Blount; Emmalea Gomberg; Frances Grappe; Chelsea Cernosek; Brittany Peoples; Jane F. Rasco; Zbigniew Krejpcio; John B. Vincent. Comparison of Tissue Metal Concentrations in Zucker Lean, Zucker Obese, and Zucker Diabetic Fatty Rats and the Effects of Chromium Supplementation on Tissue Metal Concentrations. Biological Trace Element Research 2012, 151, 373 -383.
AMA StyleHalina Staniek, Nicholas R. Rhodes, Kristin Di Bona, Ge Deng, Sharifa T. Love, Leigh Ann Pledger, Jeremy Blount, Emmalea Gomberg, Frances Grappe, Chelsea Cernosek, Brittany Peoples, Jane F. Rasco, Zbigniew Krejpcio, John B. Vincent. Comparison of Tissue Metal Concentrations in Zucker Lean, Zucker Obese, and Zucker Diabetic Fatty Rats and the Effects of Chromium Supplementation on Tissue Metal Concentrations. Biological Trace Element Research. 2012; 151 (3):373-383.
Chicago/Turabian StyleHalina Staniek; Nicholas R. Rhodes; Kristin Di Bona; Ge Deng; Sharifa T. Love; Leigh Ann Pledger; Jeremy Blount; Emmalea Gomberg; Frances Grappe; Chelsea Cernosek; Brittany Peoples; Jane F. Rasco; Zbigniew Krejpcio; John B. Vincent. 2012. "Comparison of Tissue Metal Concentrations in Zucker Lean, Zucker Obese, and Zucker Diabetic Fatty Rats and the Effects of Chromium Supplementation on Tissue Metal Concentrations." Biological Trace Element Research 151, no. 3: 373-383.
During soya seeds germination in FeSO(4) solutions their phytoferritin content is multiplied. Prepared soybean sprouts have been proposed as a safe and easily available source of iron supplementation. The preparation was compared with FeSO(4) and ferritin isolates, using rats with induced iron deficiency anaemia. After the end of the 2-week supplementation experiment, it was observed that no statistically significant differences in haemoglobin concentration, mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration existed between those animals supplemented with sprouts enriched in ferritin, ferritin isolate and FeSO(4) and healthy animals forming the control group. Moreover, the examined preparation had a beneficial influence on the recreation of ferritin reserves in both the liver and the blood serum, and also did not induce negative alterations in general growth parameters of animals. Use of an easily obtainable ferritin iron source may be a profitable alternative in supplementation due to its wide availability and food preservative properties.
Magdalena Zielińska-Dawidziak; Iwona Hertig; Dorota Piasecka-Kwiatkowska; Halina Staniek; Krzysztof W. Nowak; Tomasz Twardowski. Study on iron availability from prepared soybean sprouts using an iron-deficient rat model. Food Chemistry 2012, 135, 2622 -2627.
AMA StyleMagdalena Zielińska-Dawidziak, Iwona Hertig, Dorota Piasecka-Kwiatkowska, Halina Staniek, Krzysztof W. Nowak, Tomasz Twardowski. Study on iron availability from prepared soybean sprouts using an iron-deficient rat model. Food Chemistry. 2012; 135 (4):2622-2627.
Chicago/Turabian StyleMagdalena Zielińska-Dawidziak; Iwona Hertig; Dorota Piasecka-Kwiatkowska; Halina Staniek; Krzysztof W. Nowak; Tomasz Twardowski. 2012. "Study on iron availability from prepared soybean sprouts using an iron-deficient rat model." Food Chemistry 135, no. 4: 2622-2627.
Rafal W. Wojciak; Ewa Mojs; Ewelina Król; Zbigniew Krejpcio; Halina Staniek; Katarzyna Marcinek. The effect of different forms of food deprivation on calcium and magnesium concentrations in the serum, brain and femoral bone of female Wistar rats. Journal of Elementology 2012, 1 .
AMA StyleRafal W. Wojciak, Ewa Mojs, Ewelina Król, Zbigniew Krejpcio, Halina Staniek, Katarzyna Marcinek. The effect of different forms of food deprivation on calcium and magnesium concentrations in the serum, brain and femoral bone of female Wistar rats. Journal of Elementology. 2012; (3/2017):1.
Chicago/Turabian StyleRafal W. Wojciak; Ewa Mojs; Ewelina Król; Zbigniew Krejpcio; Halina Staniek; Katarzyna Marcinek. 2012. "The effect of different forms of food deprivation on calcium and magnesium concentrations in the serum, brain and femoral bone of female Wistar rats." Journal of Elementology , no. 3/2017: 1.