In the present study, we evaluated for the first time the photoprotective effect of fish bone bioactive peptides (FBBP) preparation isolated from silver carp (Hypophthalmichthys molitrix) discarded tissue using in vitro experimental models of skin cells exposed to ultraviolet B (UVB) irradiation and stressing agents. FBBP preparation was obtained by papain treatment of minced bones and centrifugal ultrafiltration, and the molecular weight (MW) distribution was characterized by size exclusion and reversed-phase high performance liquid chromatography (RP-HPLC). In vitro assessment of the effect of FBBP pretreatment in UVB-irradiated L929 fibroblasts and HaCaT keratinocytes revealed their cytoprotective activity. Their capacity to efficiently reduce reactive oxygen species (ROS) production and lipid peroxidation varied in a dose-dependent manner, and it was greater in fibroblasts. A decrease of proinflammatory cytokines secretion, in particular of tumor necrosis factor alpha (TNF-α), was found after FBBP pretreatment of THP-1-derived inflamed macrophages. Melanin production and tyrosinase activity investigated in UVB-irradiated Mel-Juso cells were lowered in direct relation to FBBP concentrations. FBBP fractions with high radical scavenging activity were separated by ion exchange chromatography, and two collagenic sequences were identified. All these results offer new scientific data on aquaculture fish bone-derived peptides confirming their ability to control the antioxidant, anti-inflammatory and pigmentation processes developed during UV irradiation of skin cells and recommend their use as valuable natural ingredients of photoprotective cosmeceutical products.

The aim of this study was to evaluate the photoprotective effect of fish bone-derived peptides (FBDPs) in a culture of UV-B-irradiated mouse fibroblasts and their anti-inflammatory activity in a model of stimulated human macrophages. The results showed that cell viability of FBDPs-treated and irradiated fibroblasts was higher than that of irradiated cells, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase (LDH) assay, after 25 and 60 s of treatment. Moreover, secretion of interleukin 1-beta (IL-1β) was decreased in FBDPs-treated inflamed macrophages, as determined by ELISA assay.

The association of natural or synthetic polymers with metal nanoparticles resulted in hybrid

Introduction: Nutraceuticals are food or parts of a food that provide medicinal or health benefits, including the prevention and treatment of disease.

Freshwater fish skin and bones are usually by-products of the fish processing industry.