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Tomas Kiss
Mendeleum-Institute of Genetics, Faculty of Horticulture, Mendel University in Brno, Valticka 334, 69144 Lednice, Czech Republic

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Journal article
Published: 21 May 2021 in Agronomy
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Grapevine Pinot gris virus (GPGV) is considered to be a causal agent of Grapevine Leaf Mottling and Deformation (GLMD) disease that has been reported worldwide through the grapevine-growing regions. Seven grapevines that were collected from a vineyard in the Czech Republic were tested for the presence of GPGV in leaf and phloem tissues. Each of the seven grapevines was infected by GPGV, from which sic symptoms were mostly shown without a typical mottling. The phylogeny based on RNA-dependent RNA polymerase and movement/coat protein sequences indicated the same origin of the GPGV isolates. The GPGV titer was the highest in the grapevines with the highest GLMD-like symptoms; however, some of the grapevines with milder GLMD-like symptoms had a lower GPGV titer than the asymptomatic grapevine. Soil analysis showed uneven boron content in the direct vicinity of the grapevines, while the boron content in the grapevines was more, even showing no boron deficiency. The quantitative analysis of selected gene expressions associated with boron efflux and transport only partially explained the boron content in the soil and grapevines and only in the grapevines growing in soils with the highest or lowest boron contents. The VvBor2 and VvNIP5 genes had a higher expression and VvNIP6 had a lower expression in the grapevine growing in the soil with the lowest boron content, while a low expression of VvBor1 and VvBor2 was observed in the grapevine that was grown in the soil with the highest boron content.

ACS Style

Tomáš Kiss; Mária Kocanová; Aleš Vavřiník; Dorota Tekielska; Jakub Pečenka; Eliška Hakalová; Milan Špetík; Kateřina Štůsková; Alla Mahamedi; Miroslav Baránek; Akila Berraf-Tebbal; Jana Čechová; Aleš Eichmeier. Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes. Agronomy 2021, 11, 1020 .

AMA Style

Tomáš Kiss, Mária Kocanová, Aleš Vavřiník, Dorota Tekielska, Jakub Pečenka, Eliška Hakalová, Milan Špetík, Kateřina Štůsková, Alla Mahamedi, Miroslav Baránek, Akila Berraf-Tebbal, Jana Čechová, Aleš Eichmeier. Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes. Agronomy. 2021; 11 (6):1020.

Chicago/Turabian Style

Tomáš Kiss; Mária Kocanová; Aleš Vavřiník; Dorota Tekielska; Jakub Pečenka; Eliška Hakalová; Milan Špetík; Kateřina Štůsková; Alla Mahamedi; Miroslav Baránek; Akila Berraf-Tebbal; Jana Čechová; Aleš Eichmeier. 2021. "Incidence of GLMD-Like Symptoms on Grapevines Naturally Infected by Grapevine Pinot gris virus, Boron Content and Gene Expression Analysis of Boron Metabolism Genes." Agronomy 11, no. 6: 1020.

Journal article
Published: 23 April 2021 in Sustainability
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Apricot fruits are a favorite for consumption; however, their kernels are a rich source of nutritionally interesting substances, too. Nevertheless, in processing of apricots, the kernels remain often unused. In this study, 32 cultivars of different origin were analyzed for their protein content and content of secondary metabolites (phenolics and flavonoids). The weight and taste of kernels were assessed and these data were summarized for an evaluation of the attractiveness of the studied apricot kernels. Results showed that the protein content of kernels ranged from 14.56% to 28.77% and did not depend on the origin or weight of kernel, or taste. In addition, total phenolic (63.5–1277.3 mg GAE/100 g DW) and total flavonoid (0–153.1 mg CE/100 g DW) contents and antioxidant capacity (483.4–2348.4 mg TE/100 g DW) were measured in kernels. In conclusion, the Czech hybrids LE-5959, LE-5500 and French cultivar Koolgat are prospective for kernel processing and consumption because of their high protein content and sweet taste. Hybrid LI-3-6, originating in China, showed high protein content as well but because of bitter taste could be useful rather in medicine.

ACS Style

Eliška Rampáčková; Martina Göttingerová; Pavel Gála; Tomáš Kiss; Sezai Ercişli; Tomáš Nečas. Evaluation of Protein and Antioxidant Content in Apricot Kernels as a Sustainable Additional Source of Nutrition. Sustainability 2021, 13, 4742 .

AMA Style

Eliška Rampáčková, Martina Göttingerová, Pavel Gála, Tomáš Kiss, Sezai Ercişli, Tomáš Nečas. Evaluation of Protein and Antioxidant Content in Apricot Kernels as a Sustainable Additional Source of Nutrition. Sustainability. 2021; 13 (9):4742.

Chicago/Turabian Style

Eliška Rampáčková; Martina Göttingerová; Pavel Gála; Tomáš Kiss; Sezai Ercişli; Tomáš Nečas. 2021. "Evaluation of Protein and Antioxidant Content in Apricot Kernels as a Sustainable Additional Source of Nutrition." Sustainability 13, no. 9: 4742.

Journal article
Published: 01 July 2020 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Tomáš Kiss; Vladimír Mašán; Pavel Híc. Antioxidant Capacity, Total Phenolic Compounds and Fatty Acids Composition in Walnut Oil and Bagasse Pellets Produced at Different Parameters of the Screw Press. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2020, 68, 519 -527.

AMA Style

Tomáš Kiss, Vladimír Mašán, Pavel Híc. Antioxidant Capacity, Total Phenolic Compounds and Fatty Acids Composition in Walnut Oil and Bagasse Pellets Produced at Different Parameters of the Screw Press. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2020; 68 (3):519-527.

Chicago/Turabian Style

Tomáš Kiss; Vladimír Mašán; Pavel Híc. 2020. "Antioxidant Capacity, Total Phenolic Compounds and Fatty Acids Composition in Walnut Oil and Bagasse Pellets Produced at Different Parameters of the Screw Press." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 68, no. 3: 519-527.

Research article
Published: 07 January 2020 in PLOS ONE
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A multiplex real-time PCR method based on fluorescent TaqMan® probes was developed for the simultaneous detection of the tomato pathogenic bacteria Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and bacterial spot-causing xanthomonads. The specificity of the multiplex assay was validated on 44 bacterial strains, including 32 target pathogen strains as well as closely related species and nontarget tomato pathogenic bacteria. The designed multiplex real-time PCR showed high sensitivity when positive amplification was observed for one pg of bacterial DNA in the cases of Clavibacter michiganensis subsp. michiganensis and Pseudomonas syringae pv. tomato bacteria and 100 pg for bacterial spot-causing xanthomonads. The reliability of the developed multiplex real-time PCR assay for in planta detection was verified by recognition of the target pathogens in 18 tomato plants artificially inoculated by each of the target bacteria and tomato samples from production greenhouses.

ACS Style

Eliška Peňázová; Miloň Dvořák; Lucia Ragasová; Tomas Kiss; Jakub Pečenka; Jana Čechová; Ales Eichmeier. Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants. PLOS ONE 2020, 15, e0227559 .

AMA Style

Eliška Peňázová, Miloň Dvořák, Lucia Ragasová, Tomas Kiss, Jakub Pečenka, Jana Čechová, Ales Eichmeier. Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants. PLOS ONE. 2020; 15 (1):e0227559.

Chicago/Turabian Style

Eliška Peňázová; Miloň Dvořák; Lucia Ragasová; Tomas Kiss; Jakub Pečenka; Jana Čechová; Ales Eichmeier. 2020. "Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants." PLOS ONE 15, no. 1: e0227559.

Journal article
Published: 07 November 2019 in Genes
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Diaporthe species are important pathogens, saprobes, and endophytes on grapevines. Several species are known, either as agents of pre- or post-harvest infections, as causal agents of many relevant diseases, including swelling arm, trunk cankers, leaf spots, root and fruit rots, wilts, and cane bleaching. A growing body of evidence exists that a class of small non-coding endogenous RNAs, known as microRNAs (miRNAs), play an important role in post-transcriptional gene regulation, during plant development and responses to biotic and abiotic stresses. In this study, we explored differentially expressed miRNAs in response to Diaporthe eres and Diaporthe bohemiae infection in Vitis vinifera cv. Chardonnay under in vitro conditions. We used computational methods to predict putative miRNA targets in order to explore the involvement of possible pathogen response pathways. We identified 136 known and 41 new miRNA sequence variants, likely generated through post-transcriptional modifications. In the Diaporthe eres treatment, 61 known and 17 new miRNAs were identified while in the Diaporthe bohemiae treatment, 101 known and 21 new miRNAs were revealed. Our results contribute to further understanding the role miRNAs play during plant pathogenesis, which is possibly crucial in understanding disease symptom development in grapevines infected by D. eres and D. bohemiae.

ACS Style

Ales Eichmeier; Tomas Kiss; Eliska Penazova; Jakub Pecenka; Akila Berraf-Tebbal; Miroslav Baranek; Robert Pokluda; Jana Cechova; David Gramaje; Dariusz Grzebelus. MicroRNAs in Vitis vinifera cv. Chardonnay Are Differentially Expressed in Response to Diaporthe Species. Genes 2019, 10, 905 .

AMA Style

Ales Eichmeier, Tomas Kiss, Eliska Penazova, Jakub Pecenka, Akila Berraf-Tebbal, Miroslav Baranek, Robert Pokluda, Jana Cechova, David Gramaje, Dariusz Grzebelus. MicroRNAs in Vitis vinifera cv. Chardonnay Are Differentially Expressed in Response to Diaporthe Species. Genes. 2019; 10 (11):905.

Chicago/Turabian Style

Ales Eichmeier; Tomas Kiss; Eliska Penazova; Jakub Pecenka; Akila Berraf-Tebbal; Miroslav Baranek; Robert Pokluda; Jana Cechova; David Gramaje; Dariusz Grzebelus. 2019. "MicroRNAs in Vitis vinifera cv. Chardonnay Are Differentially Expressed in Response to Diaporthe Species." Genes 10, no. 11: 905.

Journal article
Published: 25 July 2019 in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Modern high quality nursery trees pruning as knipboom grow and crop significantly better during the first years after planting in the orchard. High quality nursery trees perform better in the first years after plantation in the orchard than average or low quality nursery trees. Nurserymen are focused on production of such trees in the shortest time (one year). However, fruit tree species and varieties differ in their abilities to produce these trees. The goal of this work was to increase the quality and branching of one-year-old nursery trees at two plum and two sweet cherry varieties by the treatments of 12 different preparations of plant growth regulators (PGRs). Before lifting, branching and trunk parameters were recorded. Data showed that Ethephone+Putrescine+GA4+7 and Ethephone+Putrescine+6-BAP significantly increased almost all parameters at both plum varieties. Both sweet cherry varieties were sufficiently branching with Globaryl+epibrassinolide treatment, however the lateral shoots of ‘Celeste’ sweet cherry variety were of low quality. The same was observed after treatment with Progerbalin+4-CPPU+Putrescine. Sweet cherry ‘Kordia’ branched very well after Globaryl and TIBA+PBZ treatments too, however, the lateral shoots after treatment with the latter preparation were of low quality. ‘Kordia’ trees after Globaryl treatment were branched in 94% of cases with more than four lateral shoots.

ACS Style

Jan Wolf; Tomáš Kiss; Ivo Ondrášek; Tomas Necas. Induction of Lateral Branching of Sweet Cherry and Plum in Fruit Nursery. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2019, 47, 962 -969.

AMA Style

Jan Wolf, Tomáš Kiss, Ivo Ondrášek, Tomas Necas. Induction of Lateral Branching of Sweet Cherry and Plum in Fruit Nursery. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2019; 47 (3):962-969.

Chicago/Turabian Style

Jan Wolf; Tomáš Kiss; Ivo Ondrášek; Tomas Necas. 2019. "Induction of Lateral Branching of Sweet Cherry and Plum in Fruit Nursery." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 47, no. 3: 962-969.

Journal article
Published: 29 April 2019 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Jan Wolf; Tomas Kiss; Rostislav Venuta; Tomáš Nečas. The Use of Phytohormones in Production of Fruit Tree Rootstocks in Nursery Without Irrigation. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2019, 67, 463 -470.

AMA Style

Jan Wolf, Tomas Kiss, Rostislav Venuta, Tomáš Nečas. The Use of Phytohormones in Production of Fruit Tree Rootstocks in Nursery Without Irrigation. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2019; 67 (2):463-470.

Chicago/Turabian Style

Jan Wolf; Tomas Kiss; Rostislav Venuta; Tomáš Nečas. 2019. "The Use of Phytohormones in Production of Fruit Tree Rootstocks in Nursery Without Irrigation." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 67, no. 2: 463-470.

Journal article
Published: 28 February 2019 in Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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ACS Style

Petra Pavelková; Tomas Kiss; Tomáš Nečas. Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2019, 67, 121 -131.

AMA Style

Petra Pavelková, Tomas Kiss, Tomáš Nečas. Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2019; 67 (1):121-131.

Chicago/Turabian Style

Petra Pavelková; Tomas Kiss; Tomáš Nečas. 2019. "Evaluation of Presence and Concentration of PPV in Rootstocks Derived from Prunus davidiana (Carr.) Franch." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 67, no. 1: 121-131.

Journal article
Published: 01 January 2019 in Phytopathogenic Mollicutes
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ACS Style

Tomas Kiss; Tomas Necas. Preliminary results of auxin and brassinosteroid application on ‘Candidatus Phytoplasma prunorum’ infected plants. Phytopathogenic Mollicutes 2019, 9, 169 .

AMA Style

Tomas Kiss, Tomas Necas. Preliminary results of auxin and brassinosteroid application on ‘Candidatus Phytoplasma prunorum’ infected plants. Phytopathogenic Mollicutes. 2019; 9 (1):169.

Chicago/Turabian Style

Tomas Kiss; Tomas Necas. 2019. "Preliminary results of auxin and brassinosteroid application on ‘Candidatus Phytoplasma prunorum’ infected plants." Phytopathogenic Mollicutes 9, no. 1: 169.

Plant microbe interactions
Published: 07 September 2018 in Microbial Ecology
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“Candidatus Phytoplasma prunorum” (CPp) is a highly destructive phytopathogenic agent in many stone fruit-growing regions in Europe and the surrounding countries. In this work, we focused on documenting entire bacterial community in the phloem tissues of 60 stone fruit trees. Nested PCR and two real-time PCR assays were used to select CPp-positive (group A) and CPp-negative samples (group B). Afterwards, high-throughput amplicon sequencing was performed to assess bacterial community compositions in phloem tissues. The bacterial composition in phloem tissue consisted of 118 distinct genera, represented mainly by Pseudomonas, Acinetobacter, Methylobacterium, Sphingomonas, and Rhizobium. Statistics showed that CPp influenced the bacterial composition of infected plants (group A) and that the bacterial community depended on the geographical origin of the sample. This is the first work focusing on an analysis of the influence of CPp on the bacteria coexisting in the phloem tissues of stone fruit trees.

ACS Style

Ales Eichmeier; Tomas Kiss; Tomas Necas; Eliska Penazova; Dorota Tekielska; Marketa Bohunicka; Lucie Valentova; Radek Cmejla; Daniel Morais; Petr Baldrian. High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by “Candidatus Phytoplasma prunorum”. Microbial Ecology 2018, 77, 664 -675.

AMA Style

Ales Eichmeier, Tomas Kiss, Tomas Necas, Eliska Penazova, Dorota Tekielska, Marketa Bohunicka, Lucie Valentova, Radek Cmejla, Daniel Morais, Petr Baldrian. High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by “Candidatus Phytoplasma prunorum”. Microbial Ecology. 2018; 77 (3):664-675.

Chicago/Turabian Style

Ales Eichmeier; Tomas Kiss; Tomas Necas; Eliska Penazova; Dorota Tekielska; Marketa Bohunicka; Lucie Valentova; Radek Cmejla; Daniel Morais; Petr Baldrian. 2018. "High-Throughput Sequencing Analysis of the Bacterial Community in Stone Fruit Phloem Tissues Infected by “Candidatus Phytoplasma prunorum”." Microbial Ecology 77, no. 3: 664-675.

Journal article
Published: 01 June 2018 in Acta Horticulturae
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ACS Style

Tomas Necas; J. Wolf; Tomas Kiss; J. Láčík; T. Letocha. Use of different plant growth regulators for control of shoot branching in apple and pear trees. Acta Horticulturae 2018, 225 -232.

AMA Style

Tomas Necas, J. Wolf, Tomas Kiss, J. Láčík, T. Letocha. Use of different plant growth regulators for control of shoot branching in apple and pear trees. Acta Horticulturae. 2018; (1206):225-232.

Chicago/Turabian Style

Tomas Necas; J. Wolf; Tomas Kiss; J. Láčík; T. Letocha. 2018. "Use of different plant growth regulators for control of shoot branching in apple and pear trees." Acta Horticulturae , no. 1206: 225-232.

Journal article
Published: 29 January 2018 in Plant Pathology
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Phytoplasmas of the apple proliferation group cause diseases in various fruit trees, mainly apples, pears and apricots. Immunodominant membrane protein, or ‘imp’, shows high inter- and intraspecies gene variability, and is thus frequently used for population studies in these organisms. We used this gene to carry out a survey of ‘Candidatus Phytoplasma pyri’ imp gene diversity in the Czech Republic. In total, twelve imp genotypes that could be clustered into nine groups were identified. Using all publicly available data, 17 distinct imp genotypes (labeled A-K) were defined. Interestingly, genotypes C, H, and I were more related to ‘Ca. P. mali’ than to other ‘Ca. P. pyri’ imp genotypes, indicating that some strains may represent a transitional type between the two species. As imp likely plays an important role in host-pathogen interactions, description of its diversity in ‘Ca. P. pyri’ may help in understanding the epidemiology, symptomatology, and other aspects of pear decline disease. A simple RFLP PCR system is presented for rapid and cost effective discrimination of ‘Ca. P. pyri’ imp genotypes. This article is protected by copyright. All rights reserved.

ACS Style

M. Bohunická; L. Valentová; J. Suchá; T. Nečas; Ales Eichmeier; Tomas Kiss; R. Cmejla; Tomas Necas. Identification of 17 ‘CandidatusPhytoplasma pyri’ genotypes based on the diversity of theimpgene sequence. Plant Pathology 2018, 67, 971 -977.

AMA Style

M. Bohunická, L. Valentová, J. Suchá, T. Nečas, Ales Eichmeier, Tomas Kiss, R. Cmejla, Tomas Necas. Identification of 17 ‘CandidatusPhytoplasma pyri’ genotypes based on the diversity of theimpgene sequence. Plant Pathology. 2018; 67 (4):971-977.

Chicago/Turabian Style

M. Bohunická; L. Valentová; J. Suchá; T. Nečas; Ales Eichmeier; Tomas Kiss; R. Cmejla; Tomas Necas. 2018. "Identification of 17 ‘CandidatusPhytoplasma pyri’ genotypes based on the diversity of theimpgene sequence." Plant Pathology 67, no. 4: 971-977.

Journal article
Published: 19 September 2017 in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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‘Candidatus Phytoplasma prunorum’, the causal agent of European stone fruit yellows (ESFY), is one of the most important pathogens causing considerable economic losses in stone fruit orchards. This study evaluated trees infected and noninfected by phytoplasma ESFY of 16 apricot varieties grown in an orchard in Lednice (Czech Republic) between the years 2008-2014. Pomological traits, phenophases, pollen germination and seed viability were analysed as well as the presence of ‘Ca. P. prunorum’ in pollen, flower organs, fruit flesh, immature seeds and seedlings by nested PCR. One of the most detrimental impacts was the decreased fruit set of infected trees which occurred in 12 out of 16 studied varieties reaching an average fruit set decrease of 16.1%. Pollen germination rates also suffered, showing an average decrease by 11.78% in infected trees. In the analysis of some traits, overall significant differences were observed between the infected and noninfected trees. However, for all analysed traits in different varieties, both positive and negative influences of phytoplasma ESFY were observed. The presence of ‘Ca. P. prunorum’ in infected trees was confirmed in flower parts (only in peduncle in 70.2% of cases) and fruit parts (immature fruit flesh in 42.0% and milky kernels in 26.2% of cases), however, neither in seedlings nor in pollen.

ACS Style

Tomas Necas; Tomas Kiss; Ales Eichmeier; Jana Nečasová; Ivo Ondrášek. The Effect of Phytoplasma Disease Caused by ‘Candidatus Phytoplasma prunorum’ on the Phenological and Pomological Traits in Apricot Trees. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2017, 46, 107 -114.

AMA Style

Tomas Necas, Tomas Kiss, Ales Eichmeier, Jana Nečasová, Ivo Ondrášek. The Effect of Phytoplasma Disease Caused by ‘Candidatus Phytoplasma prunorum’ on the Phenological and Pomological Traits in Apricot Trees. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2017; 46 (1):107-114.

Chicago/Turabian Style

Tomas Necas; Tomas Kiss; Ales Eichmeier; Jana Nečasová; Ivo Ondrášek. 2017. "The Effect of Phytoplasma Disease Caused by ‘Candidatus Phytoplasma prunorum’ on the Phenological and Pomological Traits in Apricot Trees." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 46, no. 1: 107-114.

Short communication
Published: 13 September 2016 in Journal of Plant Diseases and Protection
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Twenty-four stone fruit trees showing typical symptoms of plum pox virus (PPV) were tested for PPV using ELISA, RT-PCR, real-time RT-PCR and RT-LAMP. The isolates were obtained from various regions in the Czech Republic. PPV was detected in all tested plants. Part of the coding region of the coat protein gene and adjacent non-coding region were sequenced. PPV strains of individual isolates were classified according to the obtained sequences. This study provides comprehensive evaluation of the sensitivity of four techniques used for PPV detection. Five PPV strains (D, M, W, Rec and An) were observed according to analyses of obtained sequences. The results showed that the degree of sensitivity for each detection technique varied across the four chosen methods, with the RT-LAMP technique providing very sensitive results.

ACS Style

Ales Eichmeier; Tomas Kiss; Eliška Peňázová; Jaroslav Salava; Tomáš Nečas. Comparison of four techniques for plum pox virus detection. Journal of Plant Diseases and Protection 2016, 123, 311 -315.

AMA Style

Ales Eichmeier, Tomas Kiss, Eliška Peňázová, Jaroslav Salava, Tomáš Nečas. Comparison of four techniques for plum pox virus detection. Journal of Plant Diseases and Protection. 2016; 123 (6):311-315.

Chicago/Turabian Style

Ales Eichmeier; Tomas Kiss; Eliška Peňázová; Jaroslav Salava; Tomáš Nečas. 2016. "Comparison of four techniques for plum pox virus detection." Journal of Plant Diseases and Protection 123, no. 6: 311-315.

Journal article
Published: 01 January 2016 in Genetika
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In this work, two real-time PCR protocols based on intercalating dye and two on hydrolysis probes were tested using field collected fruit tree samples infected by 16SrX group (AP, PD and ESFY) phytoplasmas. Specificity and sensitivity of protocols and amplification efficiency were the main testing parameters. Results of real-time PCR protocols were compared to nested PCR. All real-time PCR protocols confirmed their specificity of detection. All real-time PCR protocols were 10-100 times more sensitive than nested PCR. Afterall real-time PCR protocols based on hydrolysis probes were 10 times more sensitive than protocols based on intercalating dyes. Among protocols based on hydrolysis probes, slightly better detection characteristics were shown by protocol by CHRISTENSEN et al. (2004).

ACS Style

Tomas Kiss; Tomas Necas; Jana Necasova. Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas. Genetika 2016, 48, 629 -642.

AMA Style

Tomas Kiss, Tomas Necas, Jana Necasova. Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas. Genetika. 2016; 48 (2):629-642.

Chicago/Turabian Style

Tomas Kiss; Tomas Necas; Jana Necasova. 2016. "Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas." Genetika 48, no. 2: 629-642.