This page has only limited features, please log in for full access.
In vitro culture is an important biotechnological tool in plant research and an appropriate culture media is a key for a successful plant development under in vitro conditions. The use of natural compounds to improve culture media has been growing and biopolymers are interesting alternatives to synthetic compounds due to their low toxicity, biodegradability, renewability, and availability. In the present study, different culture media containing one biopolymer (chitosan, gum arabic) or a biopolymer derivative [hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC)], at 100 or 1000 mg L−1, were tested regarding their influence on the growth and physiological responses of Thymus lotocephalus in vitro culture. Cellulose-based biopolymers (HEC and CMC) and gum arabic were used for the first time in plant culture media. The results showed that CMC at 100 mg L−1 significantly improved shoot elongation while chitosan, at the highest concentration, was detrimental to T. lotocephalus. Concerning only the evaluated physiological parameters, all tested biopolymers and biopolymer derivatives are safe to plants as there was no evidence of stress-induced changes on T. lotocephalus. The rheological and microstructural features of the culture media were assessed to understand how the biopolymers and biopolymer derivatives added to the culture medium could influence shoot growth. As expected, all media presented a gel-like behaviour with minor differences in the complex viscosity at the beginning of the culture period. Most media showed increased viscosity overtime. The surface area increased with the addition of biopolymers and biopolymer derivatives to the culture media and the average pore size was considerably lower for CMC at 100 mg L−1. The smaller pores of this medium might be related to a more efficient nutrients and water uptake by T. lotocephalus shoots, leading to a significant improvement in shoot elongation. In short, this study demonstrated that the different types of biopolymers and biopolymer derivatives added to culture medium can modify their microstructure and at the right concentrations, are harmless to T. lotocephalus shoots growing in vitro, and that CMC improves shoot length.
Natacha Coelho; Alexandra Filipe; Bruno Medronho; Solange Magalhães; Carla Vitorino; Luís Alves; Sandra Gonçalves; Anabela Romano. Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus. Polysaccharides 2021, 2, 538 -553.
AMA StyleNatacha Coelho, Alexandra Filipe, Bruno Medronho, Solange Magalhães, Carla Vitorino, Luís Alves, Sandra Gonçalves, Anabela Romano. Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus. Polysaccharides. 2021; 2 (2):538-553.
Chicago/Turabian StyleNatacha Coelho; Alexandra Filipe; Bruno Medronho; Solange Magalhães; Carla Vitorino; Luís Alves; Sandra Gonçalves; Anabela Romano. 2021. "Rheological and Microstructural Features of Plant Culture Media Doped with Biopolymers: Influence on the Growth and Physiological Responses of In Vitro-Grown Shoots of Thymus lotocephalus." Polysaccharides 2, no. 2: 538-553.
Valerianaceae, the sub-family of Caprifoliaceae, contains more than 300 species of annual and perennial herbs, worldwide distributed. Several species are used for their biological properties while some are used as food. Species from the genus Valeriana have been used for their antispasmodic, relaxing, and sedative properties, which have been mainly attributed to the presence of valepotriates, borneol derivatives, and isovalerenic acid. Among this genus, the most common and employed species is Valeriana officinalis. Although valerian has been traditionally used as a mild sedative, research results are still controversial regarding the role of the different active compounds, the herbal preparations, and the dosage used. The present review is designed to summarize and critically describe the current knowledge on the different plant species belonging to Valerianaceae, their phytochemicals, their uses in the treatment of different diseases with particular emphasis on the effects on the central nervous system. The available information on this sub-family was collected from scientific databases up until year 2020. The following electronic databases were used: PubMed, Scopus, Sci Finder, Web of Science, Science Direct, NCBI, and Google Scholar. The search terms used for this review included Valerianaceae, Valeriana, Centranthus, Fedia, Patrinia, Nardostachys, Plectritis, and Valerianella, phytochemical composition, in vivo studies, Central Nervous System, neuroprotective, antidepressant, antinociceptive, anxiolytic, anxiety, preclinical and clinical studies.
Gitishree Das; Han-Seung Shin; Rosa Tundis; Sandra Gonçalves; Ourlad Tantengco; Maria Campos; Rosaria Acquaviva; Giuseppe Malfa; Anabela Romano; Joyce Robles; Mariel Clores; Jayanta-Kumar Patra. Plant Species of Sub-Family Valerianaceae—A Review on Its Effect on the Central Nervous System. Plants 2021, 10, 846 .
AMA StyleGitishree Das, Han-Seung Shin, Rosa Tundis, Sandra Gonçalves, Ourlad Tantengco, Maria Campos, Rosaria Acquaviva, Giuseppe Malfa, Anabela Romano, Joyce Robles, Mariel Clores, Jayanta-Kumar Patra. Plant Species of Sub-Family Valerianaceae—A Review on Its Effect on the Central Nervous System. Plants. 2021; 10 (5):846.
Chicago/Turabian StyleGitishree Das; Han-Seung Shin; Rosa Tundis; Sandra Gonçalves; Ourlad Tantengco; Maria Campos; Rosaria Acquaviva; Giuseppe Malfa; Anabela Romano; Joyce Robles; Mariel Clores; Jayanta-Kumar Patra. 2021. "Plant Species of Sub-Family Valerianaceae—A Review on Its Effect on the Central Nervous System." Plants 10, no. 5: 846.
The present study aimed at evaluating the effectiveness of different natural deep eutectic solvents (NADES) on the extraction of phenolic compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco, on the antioxidant activity, and acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase (Tyr) inhibitory capacities. Ten different NADES were used in this research and compared with conventional solvents. Ultrasound-assisted extraction (UAE) for 60 min proved to be the best extraction condition, and proline:lactic acid (1:1) and choline chloride:urea (1:2) extracts showed the highest total phenolic contents (56.00 ± 0.77 mgGAE/gdw) and antioxidant activity [64.35 ± 1.74 mgTE/gdw and 72.13 ± 0.97 mgTE/gdw in 2.2-diphenyl-1-picrylhydrazyl (DPPH) and 2.2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, respectively]. These extracts also exhibited enzymes inhibitory capacity particularly against Tyr and AChE. Even so, organic acid-based NADES showed to be the best extractants producing extracts with considerable ability to inhibit enzymes. Twenty-four phenolic compounds were identified by HPLC-HRMS, being rosmarinic acid, ferulic acid and salvianolic acid B the major compounds. The results confirmed that the combination of UAE and NADES provide an excellent alternative to organic solvents for sustainable and green extraction, and have huge potential for use in industrial applications involving the extraction of bioactive compounds from plants.
Inês Mansinhos; Sandra Gonçalves; Raquel Rodríguez-Solana; José Ordóñez-Díaz; José Moreno-Rojas; Anabela Romano. Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco. Antioxidants 2021, 10, 582 .
AMA StyleInês Mansinhos, Sandra Gonçalves, Raquel Rodríguez-Solana, José Ordóñez-Díaz, José Moreno-Rojas, Anabela Romano. Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco. Antioxidants. 2021; 10 (4):582.
Chicago/Turabian StyleInês Mansinhos; Sandra Gonçalves; Raquel Rodríguez-Solana; José Ordóñez-Díaz; José Moreno-Rojas; Anabela Romano. 2021. "Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco." Antioxidants 10, no. 4: 582.
BACKGROUND Mentha pulegium is an aromatic and medicinal plant used in different Mediterranean herb‐based liqueurs, whose phytochemical composition depends on the elaboration technique used. The aim of this work was to investigate differences in the sensory, chemical, and biological characteristics of pennyroyal liqueurs elaborated by hydro‐alcoholic (HI) and aqueous (AI) infusions, percolation (P), maceration (M), and distillation (D) techniques, and by the mixture of spirit with pennyroyal essential oil (EO). The volatile composition determined by gas chromatography–mass spectrometry (GC–MS) and gas chromatography coupled to flame ionization detection (GC–FID), the total phenolic (TPC) and flavonoid (TFC) contents, the antioxidant (AC), and enzyme (α‐glucosidase, tyrosinase, and acetylcholinesterase) inhibitory capacities, and the sensory analysis by napping, were evaluated in the liqueurs obtained. RESULTS Monoterpenes and sesquiterpenes were the main volatiles present in liqueurs. The M, P, HI, and AI liqueurs showed the highest TPC, TFC, and AC values; and pulegone, the main hepatotoxic chemotype, was found in concentrations that gave rise to concern. The D and EO liqueurs stand out for their acceptable pulegone content and greater tyrosinase inhibition. Similar liqueur classifications were achieved by sensory analyses, and physico‐chemical and biological analyses using multiple factor and principal component analyses, respectively. CONCLUSION These results are an important contribution to the identification of the most appropriate elaboration conditions for herbal liqueurs with the healthiest chemical composition and functional properties. © 2020 Society of Chemical Industry
Raquel Rodríguez‐Solana; Eduardo Esteves; Inês Mansinhos; Sandra Gonçalves; Efrén Pérez‐Santín; Ludovina Galego; Anabela Romano. Influence of elaboration process on chemical, biological, and sensory characteristics of E uropean pennyroyal liqueurs. Journal of the Science of Food and Agriculture 2020, 101, 4076 -4089.
AMA StyleRaquel Rodríguez‐Solana, Eduardo Esteves, Inês Mansinhos, Sandra Gonçalves, Efrén Pérez‐Santín, Ludovina Galego, Anabela Romano. Influence of elaboration process on chemical, biological, and sensory characteristics of E uropean pennyroyal liqueurs. Journal of the Science of Food and Agriculture. 2020; 101 (10):4076-4089.
Chicago/Turabian StyleRaquel Rodríguez‐Solana; Eduardo Esteves; Inês Mansinhos; Sandra Gonçalves; Efrén Pérez‐Santín; Ludovina Galego; Anabela Romano. 2020. "Influence of elaboration process on chemical, biological, and sensory characteristics of E uropean pennyroyal liqueurs." Journal of the Science of Food and Agriculture 101, no. 10: 4076-4089.
Natural products are a tremendous source of bioactive molecules with pharmacological applications. The global change context resulting from the environmental and health concerns introduce the Green Chemistry concept. This involves the use of more efficient and ecologically friendly extraction strategies (e.g., reducing the use of hazardous solvents, long extraction periods, energy consumption, and waste production) to obtain a safe and quality extract safeguarding the environment and consumers’ security. The aim of this chapter is to present an overview of the main strategies investigated in the past years to extract compounds with pharmaceutical applications from natural sources, particularly from plants and marine organisms, employing green approaches. The most important advanced extraction techniques and green solvents, employed as alternatives to replace conventional extraction approaches, their characteristics and main advantages/drawbacks, are herein discussed.
Sandra Gonçalves; Anabela Romano. Green approaches for the extraction of bioactives from natural sources for pharmaceutical applications. Green Sustainable Process for Chemical and Environmental Engineering and Science 2020, 249 -267.
AMA StyleSandra Gonçalves, Anabela Romano. Green approaches for the extraction of bioactives from natural sources for pharmaceutical applications. Green Sustainable Process for Chemical and Environmental Engineering and Science. 2020; ():249-267.
Chicago/Turabian StyleSandra Gonçalves; Anabela Romano. 2020. "Green approaches for the extraction of bioactives from natural sources for pharmaceutical applications." Green Sustainable Process for Chemical and Environmental Engineering and Science , no. : 249-267.
Extraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin–Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman’s method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.
María José Jara-Palacios; Sandra Gonçalves; Francisco J. Heredia; Dolores Hernanz; Anabela Romano. Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour. Antioxidants 2020, 9, 675 .
AMA StyleMaría José Jara-Palacios, Sandra Gonçalves, Francisco J. Heredia, Dolores Hernanz, Anabela Romano. Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour. Antioxidants. 2020; 9 (8):675.
Chicago/Turabian StyleMaría José Jara-Palacios; Sandra Gonçalves; Francisco J. Heredia; Dolores Hernanz; Anabela Romano. 2020. "Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour." Antioxidants 9, no. 8: 675.
There are increasing evidences that essential oils and phenolic compounds, the main bioactive compounds produced by aromatic plants, display a multitude of biological properties and health-promoting effects. There has been a resurgence of interest in these plants nowadays because they are considered a healthier way of complementing the human diet. Furthermore, these plants have great potential for food, cosmetic, and pharmaceutical applications. In this chapter, we present an overview of the antioxidant and neuroprotective properties of aromatic plant compounds. Overall, data hitherto reported that these plants, particularly Mediterranean species from the Lamiaceae and Apiaceae families (e.g., Coriandrum sativum L., Lavandula angustifolia Mill., Origanum vulgare L., Mentha sp., Rosmarinus officinalis L., Salvia sp., Thymus sp.), have attractive sources of compounds with preventive and/or therapeutic effects against several neurological conditions, namely anxiety, depression, epilepsy, and Alzheimer's disease, due to their capability to modulate various cellular processes of the central nervous system.
Sandra Gonçalves; Inês Mansinhos; Anabela Romano. Aromatic plants: A source of compounds with antioxidant and neuroprotective effects. Oxidative Stress and Dietary Antioxidants in Neurological Diseases 2020, 155 -173.
AMA StyleSandra Gonçalves, Inês Mansinhos, Anabela Romano. Aromatic plants: A source of compounds with antioxidant and neuroprotective effects. Oxidative Stress and Dietary Antioxidants in Neurological Diseases. 2020; ():155-173.
Chicago/Turabian StyleSandra Gonçalves; Inês Mansinhos; Anabela Romano. 2020. "Aromatic plants: A source of compounds with antioxidant and neuroprotective effects." Oxidative Stress and Dietary Antioxidants in Neurological Diseases , no. : 155-173.
Central nervous system diseases, particularly neurodegenerative maladies like Parkinson’s and Alzheimer’s diseases are the major public health concerns worldwide, since their prevalence has been increasing, and they are associated with social and financial problems. Owing the limited effectiveness and side effects of pharmacological treatments that are currently available and the fact that several factors are implicated in these diseases, novel treatments acting on multiple molecular targets are required. This chapter focuses on the beneficial effects of plant compounds against neurodegenerative diseases, specifically on the main groups of compounds, their sources, and mode of action. Reviewed results showed that there are different plant compounds with the aptitude to target simultaneously several pathological pathways and to affect the activity of numerous enzymes or genes involved in neurological diseases. Nevertheless, the key ability of the reviewed compounds is their capacity to counteract oxidative stress damages and neuronal inflammations. The available results encourage more investigations and clinical trials aimed to develop new treatments for neurodegenerative disorders based on plant compounds.
Sandra Gonçalves; Inês Mansinhos; Anabela Romano. Neuroprotective Compounds from Plant Sources and their Modes of Action: An Update. Plant-derived Bioactives 2020, 417 -440.
AMA StyleSandra Gonçalves, Inês Mansinhos, Anabela Romano. Neuroprotective Compounds from Plant Sources and their Modes of Action: An Update. Plant-derived Bioactives. 2020; ():417-440.
Chicago/Turabian StyleSandra Gonçalves; Inês Mansinhos; Anabela Romano. 2020. "Neuroprotective Compounds from Plant Sources and their Modes of Action: An Update." Plant-derived Bioactives , no. : 417-440.
Galangal is highly appreciated for its potential applications in food and medicine. In spite of its usage in food and herbal medicine in countries like Indonesia and Thailand, very limited data is accessible about its potential applications in therapeutics and pharmacology. It has been reported to be utilized in herbal medicines for treatment against diseases like hemorrhoids, abnormal menstruation, abdominal discomfort, and inflammation, among others. The widespread use of galangal in food and traditional medicine has fascinated researchers all over the world looking for its enhanced medicinal capabilities, new bioactive compounds with nutraceutical potentials, and probable uses in medicine and pharmacology. A number of literature search methods like searching the related contents with the keywords such as Alpinia sp., galangal, antiviral, cardiovascular, neuroprotective, bioactive compounds, phytochemicals etc. in the web search engines’ such as the Google search, Web of Science, Scopus, PubMed etc. were implemented. Conversely, there is no recent structured and ample review that summarizes the available data on these issues. In the current review, these topics are addressed in detail along with the investigation of scientific evidence supporting the vast applications of galangal in food and its medicinal properties, such as antiviral, cardiovascular and neuroprotective properties, together with the preclinical and clinical studies with galangal bioactive compounds.
Gitishree Das; Jayanta Kumar Patra; Sandra Gonçalves; Anabela Romano; Erick P. Gutiérrez-Grijalva; J. Basilio Heredia; Anupam Das Talukdar; Soumitra Shome; Han-Seung Shin. Galangal, the multipotent super spices: A comprehensive review. Trends in Food Science & Technology 2020, 101, 50 -62.
AMA StyleGitishree Das, Jayanta Kumar Patra, Sandra Gonçalves, Anabela Romano, Erick P. Gutiérrez-Grijalva, J. Basilio Heredia, Anupam Das Talukdar, Soumitra Shome, Han-Seung Shin. Galangal, the multipotent super spices: A comprehensive review. Trends in Food Science & Technology. 2020; 101 ():50-62.
Chicago/Turabian StyleGitishree Das; Jayanta Kumar Patra; Sandra Gonçalves; Anabela Romano; Erick P. Gutiérrez-Grijalva; J. Basilio Heredia; Anupam Das Talukdar; Soumitra Shome; Han-Seung Shin. 2020. "Galangal, the multipotent super spices: A comprehensive review." Trends in Food Science & Technology 101, no. : 50-62.
Endemic plant species are usually more vulnerable to anthropogenic threats and natural changes and, therefore, hold a higher extinction risk. The preservation of these species is a major concern on a worldwide context and in situ protection alone will not guarantee their conservation. Ex situ conservation measures must be undertaken to support the conservation of these species, and seed banking is the more efficient and cost-effective method. However, when seed banking is not an option, alternative approaches should be considered. Biotechnological tools provide new and complementary options for plant conservation including short-, medium-, and long-term strategies, and their application for plant species conservation has increased considerably in the last years. This review provides information about the status of the use biotechnology-based techniques for the conservation of endemic plant species. Particular attention is given to cryopreservation, since is the only long-term ex situ conservation strategy that can complement and support the other conservation measures. The cryopreservation of plant genetic resources is, however, more focused on crop or economically important species and few studies are available for endemic plant species. The plant material used, the cryopreservation methods employed, and the assessment of cryogenic effects are reviewed. The reasons to explain the difficulties in cryopreserving these species are discussed and new strategies are proposed to facilitate and increase the interest on this matter. We expect that further studies on the conservation of endemic plant species will increase in a near future, thus contributing to maintain these valuable genetic resources.
Natacha Coelho; Sandra Gonçalves; Anabela Romano. Endemic Plant Species Conservation: Biotechnological Approaches. Plants 2020, 9, 345 .
AMA StyleNatacha Coelho, Sandra Gonçalves, Anabela Romano. Endemic Plant Species Conservation: Biotechnological Approaches. Plants. 2020; 9 (3):345.
Chicago/Turabian StyleNatacha Coelho; Sandra Gonçalves; Anabela Romano. 2020. "Endemic Plant Species Conservation: Biotechnological Approaches." Plants 9, no. 3: 345.
Carob liqueur is a traditional Mediterranean alcoholic beverage obtained via a wide range of production techniques contributing to the different organoleptic attributes of the final product. The aim of this research was to evaluate the stability of the chemical composition and biological capacities (antioxidant and enzyme inhibition) under in vitro simulated gastrointestinal digestion of liqueurs prepared by flavouring the fig spirit with carob pulp by maceration, distillation, percolation, or aqueous and hydro-alcoholic infusions. For this purpose, the phenolic and furanic compositions, the total phenolic (TPC) and flavonoid (TFC) contents, antioxidant capacity (AC), and enzyme inhibitory potential against acethylcholinesterase, tyrosinase, α-glucosidase and α-amylase enzymes were evaluated. The content of gallic acid decreased after gastrointestinal digestion, while TPC, TFC, and AC significantly increased after each digestion phase. Overall, no significantly different enzyme inhibitions (p < 0.05) were observed among digested liqueurs, with moderate inhibition against acethylcholinesterase and tyrosinase (enzymes related with neurodegenerative diseases), and potent and low inhibitory capacities for α-glucosidase and α-amylase, respectively (ideal conditions employed in antidiabetic therapy). The study indicates that hydro-alcoholic infusion and maceration were the most appropriate methods to obtain liqueurs with higher values of the aforementioned parameters and safe levels of toxic furanics.
Raquel Rodríguez-Solana; Natacha Coelho; Antonio Santos-Rufo; Sandra Gonçalves; Efrén Pérez-Santín; Anabela Romano. The Influence of In Vitro Gastrointestinal Digestion on the Chemical Composition and Antioxidant and Enzyme Inhibitory Capacities of Carob Liqueurs Obtained with Different Elaboration Techniques. Antioxidants 2019, 8, 563 .
AMA StyleRaquel Rodríguez-Solana, Natacha Coelho, Antonio Santos-Rufo, Sandra Gonçalves, Efrén Pérez-Santín, Anabela Romano. The Influence of In Vitro Gastrointestinal Digestion on the Chemical Composition and Antioxidant and Enzyme Inhibitory Capacities of Carob Liqueurs Obtained with Different Elaboration Techniques. Antioxidants. 2019; 8 (11):563.
Chicago/Turabian StyleRaquel Rodríguez-Solana; Natacha Coelho; Antonio Santos-Rufo; Sandra Gonçalves; Efrén Pérez-Santín; Anabela Romano. 2019. "The Influence of In Vitro Gastrointestinal Digestion on the Chemical Composition and Antioxidant and Enzyme Inhibitory Capacities of Carob Liqueurs Obtained with Different Elaboration Techniques." Antioxidants 8, no. 11: 563.
Essential oils (EOs) are natural products, produced by aromatic plants, with important applications in the food, pharmaceutical, and cosmetic industries. In recent years supercritical fluid extraction with CO2 (SFE-CO2) has emerged as an efficient and environmental friendly method for the recovery of volatile oils from aromatic species, overcoming the limitations of traditional distillation methods. This chapter focuses on the main factors influencing oil recovery by SFE-CO2 from aromatic plants, and on the comparison of this technique with other extraction methods. Many factors and operational conditions greatly affect the extraction yields and the composition of the extracted oil, temperature and pressure being two of the most important factors. Furthermore, reviewed literature based on updated data show that in many plant species, comparing with other extractions methods, SFE-CO2 leads to highest extraction yields, increased oil quality, higher selectivity of the obtained compounds, and improved biological activity.
Sandra Gonçalves; Anabela Romano. Application of supercritical CO2 for enhanced oil recovery. Green Sustainable Process for Chemical and Environmental Engineering and Science 2019, 67 -84.
AMA StyleSandra Gonçalves, Anabela Romano. Application of supercritical CO2 for enhanced oil recovery. Green Sustainable Process for Chemical and Environmental Engineering and Science. 2019; ():67-84.
Chicago/Turabian StyleSandra Gonçalves; Anabela Romano. 2019. "Application of supercritical CO2 for enhanced oil recovery." Green Sustainable Process for Chemical and Environmental Engineering and Science , no. : 67-84.
Thymus lotocephalus G. Lopez & R. Morales is an aromatic species endemic to the south of Portugal with medicinal properties. The aim of this work was to evaluate the effect of elicitors, cytokinin, and different sucrose concentrations on chemical composition and antioxidant activity of extracts from in vitro regenerated shoots. Elicitors (YE: yeast extract, salicylic acid, and AgNO3) were added directly to the medium. Phytochemical analysis include evaluation of total phenolic (TPC), flavonoid (TFC), and rosmarinic acid (RAC) contents. The TPCs and TFCs were determined by Folin-Ciocalteu and aluminum chloride colorimetric assays, respectively. Rosmarinic acid (RA), the main compound found in the studied extracts, was quantified by liquid chromatography-photodiode array detector. Results showed that the cytokinin benzyladenine has an inhibitory effect on TPC, TFC and RAC, as well as on antioxidant activity. TPC was only significantly improved by the presence of YE (from 95.49 ± 3.34 to 118.34 ± 6.24 mgGAE/gextract) and TFCs were not affected by elicitation. The increase in sucrose concentration, from 2 to 4%, and the three elicitors tested, particularly YE, enhanced RACs (from 48.61 ± 0.09 in the control medium to 78.57 ± 0.99 mg/gextract in YE-elicited medium). In accordance with these results, the antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power assays was significantly greater in extracts from elicited shoots. Overall, the results obtained suggest that shoots of T. lotocephalus are a good source of antioxidant compounds and showed that the production of RA can be promoted by altering in vitro culture conditions.
Sandra Gonçalves; Inês Mansinhos; Raquel Rodríguez-Solana; Efrén Pérez Santín; Natacha Coelho; Anabela Romano. Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures. Industrial Crops and Products 2019, 137, 214 -220.
AMA StyleSandra Gonçalves, Inês Mansinhos, Raquel Rodríguez-Solana, Efrén Pérez Santín, Natacha Coelho, Anabela Romano. Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures. Industrial Crops and Products. 2019; 137 ():214-220.
Chicago/Turabian StyleSandra Gonçalves; Inês Mansinhos; Raquel Rodríguez-Solana; Efrén Pérez Santín; Natacha Coelho; Anabela Romano. 2019. "Elicitation improves rosmarinic acid content and antioxidant activity in Thymus lotocephalus shoot cultures." Industrial Crops and Products 137, no. : 214-220.
Sandra Gonçalves; Anabela Romano. Rosmarinic Acid: Sources, Properties, Applications and Biotechnological Production. Medicinal Plants 2019, 161 -171.
AMA StyleSandra Gonçalves, Anabela Romano. Rosmarinic Acid: Sources, Properties, Applications and Biotechnological Production. Medicinal Plants. 2019; ():161-171.
Chicago/Turabian StyleSandra Gonçalves; Anabela Romano. 2019. "Rosmarinic Acid: Sources, Properties, Applications and Biotechnological Production." Medicinal Plants , no. : 161-171.
Sandra Gonçalves; Anabela Romano. Ispaghula (Plantago ovata Forssk.). Nonvitamin and Nonmineral Nutritional Supplements 2019, 301 -306.
AMA StyleSandra Gonçalves, Anabela Romano. Ispaghula (Plantago ovata Forssk.). Nonvitamin and Nonmineral Nutritional Supplements. 2019; ():301-306.
Chicago/Turabian StyleSandra Gonçalves; Anabela Romano. 2019. "Ispaghula (Plantago ovata Forssk.)." Nonvitamin and Nonmineral Nutritional Supplements , no. : 301-306.
The recent interest in wild edible plants is associated with their health benefits, which are mainly due to their richness in antioxidant compounds, particularly phenolics. Nevertheless, some of these compounds are metabolized after ingestion, being transformed into metabolites frequently with lower antioxidant activity. The aim of the present study was to evaluate the influence of the digestive process on the total phenolic contents and antioxidant activity of extracts from four wild edible plants used in the Mediterranean diet (Beta maritima L., Plantago major L., Oxalis pes-caprae L. and Scolymus hispanicus L.). HPLC-DAD analysis revealed that S. hispanicus is characterized by the presence of caffeoylquinic acids, dicaffeoylquinic acids and flavonol derivatives, P. major by high amounts of verbascoside, B. maritima possesses 2,4-dihydroxybenzoic acid, 5-O-caffeoylquinic acid, quercetin derivatives and kaempferol-3-O-rutinoside, and O. pes-caprae extract contains hydroxycinnamic acids and flavone derivatives. Total phenolic contents were determined by Folin–Ciocalteu assay, and antioxidant activity by the ABTS, DPPH, ORAC and FRAP assays. Phenolic contents of P. major and S. hispanicus extracts were not affected by digestion, but they significantly decreased in B. maritima after both phases of digestion process and in O. pes-caprae after the gastric phase. The antioxidant activity results varied with the extract and the method used to evaluate the activity. Results showed that P. major extract has the highest total phenolic contents and antioxidant activity, with considerable values even after digestion, reinforcing the health benefits of this species.
Sandra Gonçalves; Elsa Moreira; Paula B. Andrade; Patrícia Valentão; Anabela Romano. Effect of in vitro gastrointestinal digestion on the total phenolic contents and antioxidant activity of wild Mediterranean edible plant extracts. European Food Research and Technology 2018, 245, 753 -762.
AMA StyleSandra Gonçalves, Elsa Moreira, Paula B. Andrade, Patrícia Valentão, Anabela Romano. Effect of in vitro gastrointestinal digestion on the total phenolic contents and antioxidant activity of wild Mediterranean edible plant extracts. European Food Research and Technology. 2018; 245 (3):753-762.
Chicago/Turabian StyleSandra Gonçalves; Elsa Moreira; Paula B. Andrade; Patrícia Valentão; Anabela Romano. 2018. "Effect of in vitro gastrointestinal digestion on the total phenolic contents and antioxidant activity of wild Mediterranean edible plant extracts." European Food Research and Technology 245, no. 3: 753-762.
Sandra Gonçalves; Anabela Romano. Production of Plant Secondary Metabolites by Using Biotechnological Tools. Secondary Metabolites - Sources and Applications 2018, 1 .
AMA StyleSandra Gonçalves, Anabela Romano. Production of Plant Secondary Metabolites by Using Biotechnological Tools. Secondary Metabolites - Sources and Applications. 2018; ():1.
Chicago/Turabian StyleSandra Gonçalves; Anabela Romano. 2018. "Production of Plant Secondary Metabolites by Using Biotechnological Tools." Secondary Metabolites - Sources and Applications , no. : 1.
The present work reports investigation on phenolic compounds, antioxidant activity and enzyme inhibitory activities (acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase and α-glucosidase) of different extracts from two Moroccan Asteraceae species; Bubonium imbricatum Cav. and Cladanthus arabicus (L.) Cass. B. imbricatum extracts contained the highest amounts of phenolics and flavonoids, and also exhibited higher antioxidant activity. In this species, the highest total phenolic (1611.13 ± 14.23 μmolGAE/gextract) and flavonoid (376.11 ± 8.22 μmolQE/gextract) contents were observed in aqueous-methanol extract obtained by maceration. Further, UHPLC–MS analysis of C. arabicus and B. imbricatum extracts revealed the presence of several flavonoids (diosmetin, luteolin, apigenin 7-glucoside, and apigenin) and phenolic acids (benzoic, protocatechuic, p-coumaric, gallic, vanillic, caffeic, ferulic and isochlorogenic acids). The antioxidant activity of the extracts was dependent of the extraction process and solvent used. Aqueous-methanol extract of B. imbricatum prepared by maceration showed the highest activity with DPPH, ABTS and FRAP tests (respectively: IC50 = 8.53 ± 0.38 μg/ml, 3461.8 ± 9.38 μmolTE/gextract and 3281.6 ± 47.43 μmolAAE/gextract). The results indicated that most of the tested extracts or essential oils exhibited activity towards the tested enzymes. Overall, the results obtained in this work indicated the two Moroccan species studied, particularly B. imbricatum, as valuable sources of natural agents beneficial for human health.
A. Aghraz; Sandra Gonçalves; R. Rodríguez-Solana; L. Ait Dra; V. Di Stefano; G. Dugo; N. Cicero; M. Larhsini; M. Markouk; A. Romano. Antioxidant activity and enzymes inhibitory properties of several extracts from two Moroccan Asteraceae species. South African Journal of Botany 2018, 118, 58 -64.
AMA StyleA. Aghraz, Sandra Gonçalves, R. Rodríguez-Solana, L. Ait Dra, V. Di Stefano, G. Dugo, N. Cicero, M. Larhsini, M. Markouk, A. Romano. Antioxidant activity and enzymes inhibitory properties of several extracts from two Moroccan Asteraceae species. South African Journal of Botany. 2018; 118 ():58-64.
Chicago/Turabian StyleA. Aghraz; Sandra Gonçalves; R. Rodríguez-Solana; L. Ait Dra; V. Di Stefano; G. Dugo; N. Cicero; M. Larhsini; M. Markouk; A. Romano. 2018. "Antioxidant activity and enzymes inhibitory properties of several extracts from two Moroccan Asteraceae species." South African Journal of Botany 118, no. : 58-64.
Plants are a tremendous source of bioactive compounds with significance in many fields from medical to agrochemical. Among plant bioactive compounds, there are some important anticancer compounds clinically used in cancer therapy or used as model compounds for chemical synthesis of potent analogs. Although these compounds have great pharmacological value, their exploitation is limited by the slow-growing nature of the plant species, the low production yields, and unpredictable variability. Moreover, due to the over collection, some anticancer species are in risk of extinction. Plant cell and tissue culture techniques have emerged as sustainable options for the propagation and conservation of medicinal plants also providing an environmentally friendly alternative method for the production of metabolites, when natural supply is limited. The aim of this chapter is to summarize the most relevant work focusing on the use of biotechnological approaches to the propagation of plants with anticancer properties and the production of valuable compounds. Our literature survey demonstrated that efforts have been mainly concentrated in species producing the compounds camptothecin, podophyllotoxin, taxol, vinblastine, and vincristine. An intensive research has been conducted on the optimization of growth conditions and application of several strategies to improve the production of anticancer compounds (particularly by elicitation) and on the elucidation of biosynthetic pathways and their regulation. In spite of all these investigations and the advantages of the production of plant compounds in vitro, there are only few examples of the production of anticancer compounds on an industrial level, and further in-depth studies are still required.
Sandra Gonçalves; Anabela Romano. Biotechnological Approaches for the Propagation of Anticancer Plants and the Production of Vital Compounds. Anticancer Plants: Natural Products and Biotechnological Implements 2018, 507 -527.
AMA StyleSandra Gonçalves, Anabela Romano. Biotechnological Approaches for the Propagation of Anticancer Plants and the Production of Vital Compounds. Anticancer Plants: Natural Products and Biotechnological Implements. 2018; ():507-527.
Chicago/Turabian StyleSandra Gonçalves; Anabela Romano. 2018. "Biotechnological Approaches for the Propagation of Anticancer Plants and the Production of Vital Compounds." Anticancer Plants: Natural Products and Biotechnological Implements , no. : 507-527.
The effect of in vitro gastrointestinal digestion on phenolic composition and antioxidant activity of different white winemaking byproducts extracts (grape pomace and its parts: seeds, skins and stems) was evaluated. Fourteen individual phenolic compounds were evaluated by UHPLC. The antioxidant activity was measured by DPPH and ORAC assays. Differences on phenolic profile and antioxidant activity were observed depending on the digestion phase, the type of byproduct, the phenolic group and the antioxidant activity assay. In general, digestion had a reducing effect on TPC and antioxidant activity; however, ORAC values of seed and stem extracts increased after digestion and some recovery indexes of the phenolic groups were very high. Results indicate that extracts from white winemaking byproducts are a reliable source of bioaccessible antioxidant compounds, which could be used as functional food ingredients.
María José Jara; Sandra Gonçalves; Dolores Hernanz; Francisco J. Heredia; Anabela Romano. Effects of in vitro gastrointestinal digestion on phenolic compounds and antioxidant activity of different white winemaking byproducts extracts. Food Research International 2018, 109, 433 -439.
AMA StyleMaría José Jara, Sandra Gonçalves, Dolores Hernanz, Francisco J. Heredia, Anabela Romano. Effects of in vitro gastrointestinal digestion on phenolic compounds and antioxidant activity of different white winemaking byproducts extracts. Food Research International. 2018; 109 ():433-439.
Chicago/Turabian StyleMaría José Jara; Sandra Gonçalves; Dolores Hernanz; Francisco J. Heredia; Anabela Romano. 2018. "Effects of in vitro gastrointestinal digestion on phenolic compounds and antioxidant activity of different white winemaking byproducts extracts." Food Research International 109, no. : 433-439.