Background: The current study was conducted to investigate the individual and combined occurrence of aflatoxin B₁ (AFB₁), deoxynivalenol (DON) and zearalenone (ZEN) in feeds from various Provinces of China during 2018 to 2020. A total of 3,507 feed samples, including 2,090 feed ingredients and 1,417 complete feed samples, were collected from different areas of China for mycotoxins analysis. Results: The individual contamination of AFB₁, DON and ZEN were present in more than 81.9%, 96.4% and 96.9% of feed samples, respectively, with average concentration ranges of AFB₁ between 1.2–27.4 μg/kg, DON between 458.0–1,925.4 μg/kg and ZEN between 48.1–326.8 μg/kg. Notably, 0.9%, 0.5% and 0.1% of feed ingredients, and 1.2–12.8%, 0.9–2.9% and 0–8.9% of complete feeds for pigs, poultry and ruminants with AFB₁, ZEN and DON that exceeded China’s safety standards, respectively. Moreover, more than 81.5% of feed ingredients and 95.7% of complete feeds were co-contaminated with various combinations of these mycotoxins. Conclusion: This study indicates that the feeds in China were universally contaminated with AFB₁, DON and ZEN during the past 3 years. These findings highlight the significance of monitoring mycotoxin contaminant levels in the domestic animal feed, and the importance of carrying out feed administration and remediation strategies for mycotoxin control.

Mycotoxins are secondary metabolites of different species of fungi. Aflatoxin B₁ (AFB₁), deoxynivalenol (DON), zearalenone (ZEN) and fumonisin B₁ (FB₁) are the main mycotoxins contaminating animal feedstuffs. These mycotoxins can primarily induce hepatotoxicity, immunotoxicity, neurotoxicity and nephrotoxicity, consequently cause adverse effects on the health and performance of animals. Therefore, physical, chemical, biological and nutritional regulation approaches have been developed as primary strategies for the decontamination and detoxification of these mycotoxins in the feed industry. Meanwhile, each of these techniques has its drawbacks, including inefficient, costly, or impractically applied on large scale. This review summarized the advantages and disadvantages of the different remediation strategies, as well as updates of the research progress of these strategies for AFB₁, DON, ZEN and FB₁ control in the feed industry.

The objective of this study was to investigate the individual and combined contamination of aflatoxin B₁ (AFB₁), zearalenone (ZEN) and deoxynivalenol (DON) in feedstuffs from different Provinces of China between 2016 and 2017. A total of 1569 samples, including 742 feed ingredients and 827 complete pig feed samples, were collected from various regions of China for mycotoxins analysis. The results showed that individual occurrence rates of AFB₁, ZEN, and DON were more than 83.3%, 88%, and 74.5%, respectively, in all the tested samples. DON was the most prevalent contaminant, followed by ZEN and AFB₁, with the average concentrations ranging from 450.0–4381.5 μg/kg, 2.3–729.2 μg/kg, and 1.3–10.0 μg/kg, respectively. Notable, 38.2%, 10.8%, and 0.6% of complete pig feeds were contaminated with DON, ZEN, and AFB₁ over China’s regulatory limits, respectively. Moreover, over 75.0% analyzed samples were co-contaminated with two or three mycotoxins. In conclusion, the current study revealed that the feedstuffs in China were severely contaminated with DON, followed by ZEN and AFB₁ during the past two years. These findings highlight the importance of monitoring mycotoxins in livestock feed and implementing feed management and bioremediation strategies to reduce mycotoxin exposure.

Mycotoxins are toxic secondary metabolites produced by filamentous fungi that are commonly detected as natural contaminants in agricultural commodities worldwide. Mycotoxin exposure can lead to mycotoxicosis in both animals and humans when found in animal feeds and food products, and at lower concentrations can affect animal performance by disrupting nutrient digestion, absorption, metabolism, and animal physiology. Thus, mycotoxin contamination of animal feeds represents a significant issue to the livestock industry and is a health threat to food animals. Since prevention of mycotoxin formation is difficult to undertake to avoid contamination, mitigation strategies are needed. This review explores how the mycotoxins aflatoxins, deoxynivalenol, zearalenone, fumonisins and ochratoxin A impose nutritional and metabolic effects on food animals and summarizes mitigation strategies to reduce the risk of mycotoxicity.

Aflatoxicosis is a grave threat to the poultry industry. Dietary supplementation with antioxidants showed a great potential in enhancing the immune system; hence, protecting animals against aflatoxin B₁-induced toxicity. Grape seed proanthocyanidin extract (GSPE) one of the most well-known and powerful antioxidants. Therefore, the purpose of this research was to investigate the effectiveness of GSPE in the detoxification of AFB₁ in broilers. A total of 300 one-day-old Cobb chicks were randomly allocated into five treatments of six replicates (10 birds per replicate), fed ad libitum for four weeks with the following dietary treatments: 1. Basal diet (control); 2. Basal diet + 1 mg/kg AFB₁ contaminated corn (AFB₁); 3. Basal diet + GSPE 250 mg/kg; (GSPE 250 mg/kg) 4. Basal diet + AFB₁ (1 mg/kg) + GSPE 250 mg/kg; (AFB₁ + GSPE 250 mg/kg) 5. Basal diet + AFB₁ (1mg/kg) + GSPE 500 mg/kg, (AFB₁ + GSPE 500 mg/kg). When compared with the control group, feeding broilers with AFB₁ alone significantly reduced growth performance, serum immunoglobulin contents, negatively altered serum biochemical contents, and enzyme activities, and induced histopathological lesion in the liver. In addition, AFB₁ significantly increased malondialdehyde content and decreased total superoxide dismutase, catalase, glutathione peroxide, glutathione-S transferase, glutathione reductase activities, and glutathione concentration within the liver and serum. The supplementation of GSPE (250 and 500 mg/kg) to AFB₁ contaminated diet reduced AFB₁ residue in the liver and significantly mitigated AFB₁ negative effects. From these results, it can be concluded that dietary supplementation of GSPE has protective effects against aflatoxicosis caused by AFB₁ in broiler chickens.