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Lentil (Lens culinaris Medik) is an important component of the human diet due to its high mineral and protein contents. Abiotic stresses, i.e., drought, decreases plant growth and yield. Drought causes the synthesis of reactive oxygen species, which decrease a plant’s starch contents and growth. However, ACC-deaminase (1-aminocyclopropane-1-carboxylate deaminase) producing rhizobacteria can alleviate drought stress by decreasing ethylene levels. On the other hand, caffeic acid (CA) can also positively affect cell expansion and turgor pressure maintenance under drought stress. Therefore, the current study was planned with an aim to assess the effect of CA (0, 20, 50 and 100 ppm) and ACC-deaminase rhizobacteria (Lysinibacillus fusiform, Bacillus amyloliquefaciens) on lentils under drought stress. The combined application of CA and ACC-deaminase containing rhizobacteria significantly improved plant height (55%), number of pods per plant (51%), 1000-grain weight (45%), nitrogen concentration (56%), phosphorus concentration (19%), potassium concentration (21%), chlorophyll (54%), relative water contents RWC (60%) and protein contents (55%). A significant decrease in electrolyte leakage (30%), proline contents (44%), and hydrogen peroxide contents (54%), along with an improvement in cell membrane stability (34% over control) validated the combined use of CA and rhizobacteria. In conclusion, co-application of CA (20 ppm) and ACC-deaminase producing rhizobacteria can significantly improve plant growth and yield for farmers under drought stress. More investigations are suggested at the field level to select the best rhizobacteria and CA level for lentils under drought.
Muhammad Zafar-Ul-Hye; Muhammad Naeem Akbar; Yasir Iftikhar; Mazhar Abbas; Atiqa Zahid; Shah Fahad; Rahul Datta; Muqarrab Ali; Abdallah M. Elgorban; Mohammad Javed Ansari; Subhan Danish. Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants. Sustainability 2021, 13, 9603 .
AMA StyleMuhammad Zafar-Ul-Hye, Muhammad Naeem Akbar, Yasir Iftikhar, Mazhar Abbas, Atiqa Zahid, Shah Fahad, Rahul Datta, Muqarrab Ali, Abdallah M. Elgorban, Mohammad Javed Ansari, Subhan Danish. Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants. Sustainability. 2021; 13 (17):9603.
Chicago/Turabian StyleMuhammad Zafar-Ul-Hye; Muhammad Naeem Akbar; Yasir Iftikhar; Mazhar Abbas; Atiqa Zahid; Shah Fahad; Rahul Datta; Muqarrab Ali; Abdallah M. Elgorban; Mohammad Javed Ansari; Subhan Danish. 2021. "Rhizobacteria Inoculation and Caffeic Acid Alleviated Drought Stress in Lentil Plants." Sustainability 13, no. 17: 9603.
The edible parts of the plants Camellia sinensis, Vitis vinifera and Withania somnifera were extensively used in ancient practices such as Ayurveda, owing to their potent biomedical significance. They are very rich in secondary metabolites such as polyphenols, which are very good antioxidants and exhibit anti-carcinogenic properties. This study aims to evaluate the anti-cancerous properties of these plant crude extracts on human liver cancer HepG2 cells. The leaves of Camellia sinensis, Withania somnifera and the seeds of Vitis vinifera were collected and methanolic extracts were prepared. Then, these extracts were subjected to DPPH, α- amylase assays to determine the antioxidant properties. A MTT assay was performed to investigate the viability of the extracts of HepG2 cells, and the mode of cell death was detected by Ao/EtBr staining and flow cytometry with PI Annexin- V FITC dual staining. Then, the protein expression of BAX and BCl2 was studied using fluorescent dye to determine the regulation of the BAX and BCl2 genes. We observed that all the three extracts showed the presence of bioactive compounds such as polyphenols or phytochemicals. The W. somnifera bioactive compounds were found to have the highest anti-proliferative activity on human liver cancer cells.
Deepthi Venkatachalapathy; Chandan Shivamallu; Shashanka Prasad; Gopenath Thangaraj Saradha; Parthiban Rudrapathy; Raghavendra Amachawadi; Sharanagouda Patil; Asad Syed; Abdallah Elgorban; Ali Bahkali; Shiva Kollur; Kanthesh Basalingappa. Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line. Molecules 2021, 26, 4593 .
AMA StyleDeepthi Venkatachalapathy, Chandan Shivamallu, Shashanka Prasad, Gopenath Thangaraj Saradha, Parthiban Rudrapathy, Raghavendra Amachawadi, Sharanagouda Patil, Asad Syed, Abdallah Elgorban, Ali Bahkali, Shiva Kollur, Kanthesh Basalingappa. Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line. Molecules. 2021; 26 (15):4593.
Chicago/Turabian StyleDeepthi Venkatachalapathy; Chandan Shivamallu; Shashanka Prasad; Gopenath Thangaraj Saradha; Parthiban Rudrapathy; Raghavendra Amachawadi; Sharanagouda Patil; Asad Syed; Abdallah Elgorban; Ali Bahkali; Shiva Kollur; Kanthesh Basalingappa. 2021. "Assessment of Chemopreventive Potential of the Plant Extracts against Liver Cancer Using HepG2 Cell Line." Molecules 26, no. 15: 4593.
Trivalent chromium ions are the abundant toxic metal, released to environment by various industrial effluent discharges. A sensitive and specific SPR technique for Cr(III) detection in trace levels from aqueous sample was developed in the present study. Here, Ag decorated Au nanoparticles (NPs) was functionalized with citrate by chemical-reduction technique. The Ag–Au NPs probe was analyzed via UV–visible spectrophotometer, TEM, EDAX, FTIR, DLS, and zetasizer. The Ag–Au NPs zeta potential was −36.66 mV and the particle size was 19 nm. The developed probe shown drastic variation in colour from dark pink to colourless with response time of 60 s after the interaction with Cr(III) ions. The absorbance at A526 showed good linearity with Cr(III) concentrations (10 nM - 100 µM). The LOD of Ag–Au NPs probe for Cr(III) detection was found to be 0.4 nM. The Ag–Au NPs sensor showed high specificity towards Cr(III) ions even in presence with competitive metal ions. In acidic pH and at temperature range of 25–50 °C the probe worked effectively. At last, the fabricated probe was applied for Cr(III) ion estimation in Cr(III) augmented environmental samples and the detection accuracy was determined by comparing with atomic absorption spectrophotometric analysis. Thus, the reported suggest that Ag–Au NPs could be applied for the selective detection of Cr(III) from environmental samples with excellent accuracy.
Asad Syed; Gajanan Ghodake; Abdallah M. Elgorban; Ali H. Bahkali; Fetoon Alkhelaiwi. Development of Ag decorated Au core-shell nanospheres for the detection of Cr(III) from environmental sample. Optical Materials 2021, 120, 111409 .
AMA StyleAsad Syed, Gajanan Ghodake, Abdallah M. Elgorban, Ali H. Bahkali, Fetoon Alkhelaiwi. Development of Ag decorated Au core-shell nanospheres for the detection of Cr(III) from environmental sample. Optical Materials. 2021; 120 ():111409.
Chicago/Turabian StyleAsad Syed; Gajanan Ghodake; Abdallah M. Elgorban; Ali H. Bahkali; Fetoon Alkhelaiwi. 2021. "Development of Ag decorated Au core-shell nanospheres for the detection of Cr(III) from environmental sample." Optical Materials 120, no. : 111409.
Salinity significantly impacts the growth, development, and reproductive biology of various crops such as vegetables. The cultivable area is reduced due to the accumulation of salts and chemicals currently in use and is not amenable to a large extent to avoid such abiotic stress factors. The addition of microbes enriches the soil without any adverse effects. The effects of microbial consortia comprising Bacillus sp., Delftia sp., Enterobacter sp., Achromobacter sp., was evaluated on the growth and mineral uptake in tomatoes (Solanum Lycopersicum L.) under salt stress and normal soil conditions. Salinity treatments comprising Ec 0, 2, 5, and 8 dS/m were established by mixing soil with seawater until the desired Ec was achieved. The seedlings were transplanted in the pots of the respective pH and were inoculated with microbial consortia. After sufficient growth, these seedlings were transplanted in soil seedling trays. The measurement of soil minerals such as Na, K, Ca, Mg, Cu, Mn, and pH and the Ec were evaluated and compared with the control 0 days, 15 days, and 35 days after inoculation. The results were found to be non-significant for the soil parameters. In the uninoculated seedlings’ (control) seedling trays, salt treatment significantly affected leaf, shoot, root dry weight, shoot height, number of secondary roots, chlorophyll, and mineral contents. While bacterized seedlings sown under saline soil significantly increased leaf (105.17%), shoot (105.62%), root (109.06%) dry weight, leaf number (75.68%), shoot length (92.95%), root length (146.14%), secondary roots (91.23%), and chlorophyll content (−61.49%) as compared to the control (without consortia). The Na and K intake were higher even in the presence of the microbes, but the beneficial effect of the microbe helps plants sustain in the saline environment. The inoculation of microbial consortia produced more secondary roots, which accumulate more minerals and transport substances to the different parts of the plant; thus, it produced higher biomass and growth. Results of the present study revealed that the treatment with microbial consortia could alleviate the deleterious effects of salinity stress and improve the growth of tomato plants under salinity stress. Microbial consortia appear to be the best alternative and cost-effective and sustainable approach for managing soil salinity and improving plant growth under salt stress conditions.
Chintan Kapadia; R. Sayyed; Hesham El Enshasy; Harihar Vaidya; Deepshika Sharma; Nafisa Patel; Roslinda Malek; Asad Syed; Abdallah Elgorban; Khurshid Ahmad; Ali Zuan. Halotolerant Microbial Consortia for Sustainable Mitigation of Salinity Stress, Growth Promotion, and Mineral Uptake in Tomato Plants and Soil Nutrient Enrichment. Sustainability 2021, 13, 8369 .
AMA StyleChintan Kapadia, R. Sayyed, Hesham El Enshasy, Harihar Vaidya, Deepshika Sharma, Nafisa Patel, Roslinda Malek, Asad Syed, Abdallah Elgorban, Khurshid Ahmad, Ali Zuan. Halotolerant Microbial Consortia for Sustainable Mitigation of Salinity Stress, Growth Promotion, and Mineral Uptake in Tomato Plants and Soil Nutrient Enrichment. Sustainability. 2021; 13 (15):8369.
Chicago/Turabian StyleChintan Kapadia; R. Sayyed; Hesham El Enshasy; Harihar Vaidya; Deepshika Sharma; Nafisa Patel; Roslinda Malek; Asad Syed; Abdallah Elgorban; Khurshid Ahmad; Ali Zuan. 2021. "Halotolerant Microbial Consortia for Sustainable Mitigation of Salinity Stress, Growth Promotion, and Mineral Uptake in Tomato Plants and Soil Nutrient Enrichment." Sustainability 13, no. 15: 8369.
Camellia sinensis is one of the major crops grown in Taiwan and has been widely cultivated around the island. Tea leaves are prone to various fungal infections, and leaf spot is considered one of the major diseases in Taiwan tea fields. As part of a survey on fungal species causing leaf spots on tea leaves in Taiwan, 19 fungal strains morphologically similar to the genus Diaporthe were collected. ITS (internal transcribed spacer), tef1-α (translation elongation factor 1-α), tub2 (beta-tubulin), and cal (calmodulin) gene regions were used to construct phylogenetic trees and determine the evolutionary relationships among the collected strains. In total, six Diaporthe species, including one new species, Diaporthe hsinchuensis, were identified as linked with leaf spot of C. sinensis in Taiwan based on both phenotypic characters and phylogeny. These species were further characterized in terms of their pathogenicity, temperature, and pH requirements under laboratory conditions. Diaporthe tulliensis, D. passiflorae, and D. perseae were isolated from C. sinensis for the first time. Furthermore, pathogenicity tests revealed that, with wound inoculation, only D. hongkongensis was pathogenic on tea leaves. This investigation delivers the first assessment of Diaporthe taxa related to leaf spots on tea in Taiwan.
Hiran Ariyawansa; Ichen Tsai; Jian-Yuan Wang; Patchareeya Withee; Medsaii Tanjira; Shiou-Ruei Lin; Nakarin Suwannarach; Jaturong Kumla; Abdallah Elgorban; Ratchadawan Cheewangkoon. Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan. Plants 2021, 10, 1434 .
AMA StyleHiran Ariyawansa, Ichen Tsai, Jian-Yuan Wang, Patchareeya Withee, Medsaii Tanjira, Shiou-Ruei Lin, Nakarin Suwannarach, Jaturong Kumla, Abdallah Elgorban, Ratchadawan Cheewangkoon. Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan. Plants. 2021; 10 (7):1434.
Chicago/Turabian StyleHiran Ariyawansa; Ichen Tsai; Jian-Yuan Wang; Patchareeya Withee; Medsaii Tanjira; Shiou-Ruei Lin; Nakarin Suwannarach; Jaturong Kumla; Abdallah Elgorban; Ratchadawan Cheewangkoon. 2021. "Molecular Phylogenetic Diversity and Biological Characterization of Diaporthe Species Associated with Leaf Spots of Camellia sinensis in Taiwan." Plants 10, no. 7: 1434.
Nosocomial infections are a notorious subset of infectious diseases, varying between 10% and 20% prevalence worldwide. The infections are concomitant with various treatment complications, multiple-drug resistance, and a high degree of virulence. Klebsiella pneumoniae is a gram-negative bacteria of nosocomial importance. Our current study is gauged to reason and understand why, despite treatment with cutting-edge medicines and technology, the K. pneumoniae remains elusive. Using various in silico tools, the KPHS_00890 hypothetical protein of K. pneumoniae subsp. pneumoniae HS11286 was identified and annotated. A thorough investigation revealed that KPHS_00890 hypothetical protein is a bifunctional 5′-nucleotidase, an enzyme catalyzing the degradation of nucleotides to nucleosides. Scrutiny and review of the 5′-nucleotidase function across various species ascertained its pertinent role in immune evasion, by suppressing inflammatory responses. Thus, having identified the KPHS_00890 hypothetical protein of K. pneumoniae subsp. pneumoniae HS11286 as a 5′-nucleotidase, we propose that it may be involved in an immune evasion strategy during infection pathogenesis.
Pavan Heggadadevanakote Kendaganna; Chandan Shivamallu; Govindaraju Shruthi; Mekhala Nagabushan Chitagudigi; Sushma Pradeep; Prashantha Karunakar; Amachawadi G. Raghavendra; Sharanagouda S. Patil; Asad Syed; Abdallah M. Elgorban; Ali H. Bahkali; Ravindra Veerapur; Shiva Prasad Kollur. In silico screening and validation of KPHS_00890 protein of Klebsiella pneumoniae proteome: An application to bacterial resistance and pathogenesis. Journal of King Saud University - Science 2021, 33, 101537 .
AMA StylePavan Heggadadevanakote Kendaganna, Chandan Shivamallu, Govindaraju Shruthi, Mekhala Nagabushan Chitagudigi, Sushma Pradeep, Prashantha Karunakar, Amachawadi G. Raghavendra, Sharanagouda S. Patil, Asad Syed, Abdallah M. Elgorban, Ali H. Bahkali, Ravindra Veerapur, Shiva Prasad Kollur. In silico screening and validation of KPHS_00890 protein of Klebsiella pneumoniae proteome: An application to bacterial resistance and pathogenesis. Journal of King Saud University - Science. 2021; 33 (6):101537.
Chicago/Turabian StylePavan Heggadadevanakote Kendaganna; Chandan Shivamallu; Govindaraju Shruthi; Mekhala Nagabushan Chitagudigi; Sushma Pradeep; Prashantha Karunakar; Amachawadi G. Raghavendra; Sharanagouda S. Patil; Asad Syed; Abdallah M. Elgorban; Ali H. Bahkali; Ravindra Veerapur; Shiva Prasad Kollur. 2021. "In silico screening and validation of KPHS_00890 protein of Klebsiella pneumoniae proteome: An application to bacterial resistance and pathogenesis." Journal of King Saud University - Science 33, no. 6: 101537.
Welsh onion (Allium fistulosum L.) is one of the main and oldest vegetable crops grown in Taiwan. A severe epidemic of leaf blight in Welsh onion caused by a Stemphylium-like pathogen was found in Sanxing, Taiwan, from 2018 to 2020. However, correct species identification, biology and control of Stemphylium leaf blight (SLB) of Welsh onion is not well established. Therefore, the main objective of this study was to investigate the causal agent of SLB in Sanxing and evaluate the in vitro sensitivity of Stemphylium-like pathogen to commonly used fungicides. Phylogenetic analysis based on combining the internal transcribed spacer (ITS) region, and glyceraldedyhe-3-phosphate dehydrogenase (gapdh) and calmodulin (cmdA) gene sequences together with morphological features, identified S. vesicarium as associated with SLB in Sanxing. When inoculated onto Welsh onion leaves, the isolates caused symptoms identical to those seen in the field and S. vesicarium was reisolated, confirming Koch’s postulates. We observed a higher incidence of SLB symptoms on the oldest leaves compared with younger leaves. The maximum and minimum temperatures for in vitro mycelial growth and conidial germination (%) of S. vesicarium were 20 to 30°C and 5°C respectively. Sixteen fungicides were tested for their effectiveness to reduce the mycelial growth and conidial germination of S. vesicarium in vitro. Boscalid + pyraclostrobin, fluopyram, fluxapyroxad, fluxapyroxad + pyraclostrobin were highly effective at reducing mycelial growth and conidial germination in S. vesicarium. However, strobilurin fungicides (azoxystrobin and kresoxim-methyl) commonly used in Welsh onion production in Sanxing were ineffective. This study discusses the emergence of SLB caused by S. vesicarium in the foliar disease complex affecting Welsh onion and the management of the disease using fungicides with different modes of action in Taiwan. The research will underpin the sustainable management of SLB in Sanxing, Taiwan, however further field assessments of the fungicides are warranted.
Chun-Hsiang Wang; Yi Chen Tsai; Chia-Lin Chung; Yu-Chen Lin; Wen Tung Hsieh; Ting-Hsuan Hung; Nakarin Suwannarach; Ratchadawan Cheewangkoon; Abdallah Elgorban; Hiran Anjana Ariyawansa. Stemphylium Leaf Blight of Welsh onion (Allium fistulosum): An Emerging Disease in Sanxing, Taiwan. Plant Disease 2021, 1 .
AMA StyleChun-Hsiang Wang, Yi Chen Tsai, Chia-Lin Chung, Yu-Chen Lin, Wen Tung Hsieh, Ting-Hsuan Hung, Nakarin Suwannarach, Ratchadawan Cheewangkoon, Abdallah Elgorban, Hiran Anjana Ariyawansa. Stemphylium Leaf Blight of Welsh onion (Allium fistulosum): An Emerging Disease in Sanxing, Taiwan. Plant Disease. 2021; ():1.
Chicago/Turabian StyleChun-Hsiang Wang; Yi Chen Tsai; Chia-Lin Chung; Yu-Chen Lin; Wen Tung Hsieh; Ting-Hsuan Hung; Nakarin Suwannarach; Ratchadawan Cheewangkoon; Abdallah Elgorban; Hiran Anjana Ariyawansa. 2021. "Stemphylium Leaf Blight of Welsh onion (Allium fistulosum): An Emerging Disease in Sanxing, Taiwan." Plant Disease , no. : 1.
In the present work, CaWO4/Ag2MoO4 nanohybrid was synthesized by sono-chemical method. Fourier-transform infrared spectroscopy (FTIR), UV–visible diffuse reflectance spectroscopy (UV–vis DRS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), high resolution transmission electron microscopy (HRTEM), energy dispersive X-ray spectroscopy (EDAX) and BET surface area analyzer were used to characterize the structure and optical properties of CaWO4/Ag2MoO4 nanohybrid. The HRTEM image represents the formation of CaWO4/Ag2MoO4 nanohybrid with clear facets and narrow size range. The XRD results showed that CaWO4/Ag2MoO4 nanohybrid composed of combined phases of CaWO4 and Ag2MoO4. A red-shift was observed in the optical absorption of nanohybrid after loading Ag2MoO4 on CaWO4. The photodegradation of MB by CaWO4/Ag2MoO4 nanohybrid (91.4%) showed excellent performance when compared with the pure Ag2MoO4 (59.6%) and CaWO4 (14.3%) under visible light. The scavenging test revealed that the key species responsible for the degradation of MB dye was hydroxide radical (•OH). The degradation of MB was well fitted to the pseudo-first-order kinetics with a high regression coefficient value (R2 > 0.95). The photostability and reusability of the nanohybrid was studied and the nanohybrid showed almost same degradation efficiency even at 6th cycle. The nanohybrid showed enhanced antimicrobial activity against Escherichia coli and Bacillus subtilis. Therefore, CaWO4/Ag2MoO4 nanohybrid photocatalyst can be used as an effective candidate for environmental remediation through photocatalysis and bactericidal applications.
Asad Syed; Hind A. Al-Shwaiman; Manal M. Al Khulaifi; Raghad R. Al Zahrani; Fahad N. Almajhdi; Abdallah M. Elgorban. Integrating plasmonic effect and nano-heterojunction formation for boosted light harvesting and photocatalytic performance using CaWO4/Ag2MoO4 and its antibacterial applications. Materials Science in Semiconductor Processing 2021, 133, 105921 .
AMA StyleAsad Syed, Hind A. Al-Shwaiman, Manal M. Al Khulaifi, Raghad R. Al Zahrani, Fahad N. Almajhdi, Abdallah M. Elgorban. Integrating plasmonic effect and nano-heterojunction formation for boosted light harvesting and photocatalytic performance using CaWO4/Ag2MoO4 and its antibacterial applications. Materials Science in Semiconductor Processing. 2021; 133 ():105921.
Chicago/Turabian StyleAsad Syed; Hind A. Al-Shwaiman; Manal M. Al Khulaifi; Raghad R. Al Zahrani; Fahad N. Almajhdi; Abdallah M. Elgorban. 2021. "Integrating plasmonic effect and nano-heterojunction formation for boosted light harvesting and photocatalytic performance using CaWO4/Ag2MoO4 and its antibacterial applications." Materials Science in Semiconductor Processing 133, no. : 105921.
The present study was aimed to test cholesterol reduction and vitamin B12 production abilities of the isolated lactic acid bacteria (LAB). Three LAB isolates, namely, Enterococcus faecium (EF), Enterococcus faecium (Chole1), and Lactobacillus pentosus (7MP), having probiotic potential, were isolated from yoghurt. These isolates were screened for bile salt hydrolase (BSH) activity, cholesterol reduction property in MRS broth, and the production of vitamin B12. The present study revealed that the isolate 7MP possesses the highest potential of (48%) cholesterol reduction compared to the other isolates. The isolates EF and Chole1 produced a good amount of (1 ng/mL) vitamin B12. These isolates were identified by 16S rRNA gene sequencing and confirmed by MALD_TOF analysis. Thus, the use of these LAB isolates for yoghurt-making can offer the value addition of lowering cholesterol and vitamin B12 fortification in fermented food.
Rajan Walhe; Sham Diwanay; Milind Patole; R. Sayyed; Hind Al-Shwaiman; Manal Alkhulaifi; Abdallah Elgorban; Subhan Danish; Rahul Datta. Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt. Sustainability 2021, 13, 5853 .
AMA StyleRajan Walhe, Sham Diwanay, Milind Patole, R. Sayyed, Hind Al-Shwaiman, Manal Alkhulaifi, Abdallah Elgorban, Subhan Danish, Rahul Datta. Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt. Sustainability. 2021; 13 (11):5853.
Chicago/Turabian StyleRajan Walhe; Sham Diwanay; Milind Patole; R. Sayyed; Hind Al-Shwaiman; Manal Alkhulaifi; Abdallah Elgorban; Subhan Danish; Rahul Datta. 2021. "Cholesterol Reduction and Vitamin B12 Production Study on Enterococcus faecium and Lactobacillus pentosus Isolated from Yoghurt." Sustainability 13, no. 11: 5853.
The outbreak of novel coronavirus strain (Covid-19) with a high pandemic threat has predict grave public health and economic concerns. This virus, originating from the Wuhan region in China has spread worldwide affecting millions with no registered persuasive targeted therapy. In this paper, we analyze the three important proteins encoded by the virus, envelope protein 5 × 29, RNA binding nucleocapsid protein 1SSK, and spike glycoprotein 6ACD, for an effective virion accumulation, and remdesivir was the first drug approved by the FDA and EMA for the treatment of COVID-19 cases that require hospitalization, there is still much controversy about its efficacy and also an alternative for novel phytochemicals, deoxynojirimycin, trigoneoside IB, and octanoic acid. The in-silico evaluations were conducted using the PyRx virtual screening tools which lead to the target based on high binding affinity. Trigoneoside IB, derived from Trigonella foenum-graecum (Fenugreek), showed the highest binding affinity and stable interaction with the amino acid residues present in active sites of Covid-19 proteins. Meanwhile, the other two compounds derived from Morus alba (Mulberry) and Morinda citrifolia (Noni), as well as the anti-HIV remdesivir drug exhibited good binding affinity and favorable ADME properties. Thereby offering scope for validation of the new therapeutic components for their in vitro and in vivo efficacy against the Covid-19 proteins.
Chandan Dharmashekara; Sushma Pradeep; Shashanka K Prasad; Anisha S Jain; Asad Syed; Kollur Shiva Prasad; Sharanagouda S Patil; Mallikarjun S Beelagi; Chandrashekar Srinivasa; Chandan Shivamallu. Virtual screening of potential phyto-candidates as therapeutic leads against SARS-CoV-2 infection. Environmental Challenges 2021, 4, 100136 -100136.
AMA StyleChandan Dharmashekara, Sushma Pradeep, Shashanka K Prasad, Anisha S Jain, Asad Syed, Kollur Shiva Prasad, Sharanagouda S Patil, Mallikarjun S Beelagi, Chandrashekar Srinivasa, Chandan Shivamallu. Virtual screening of potential phyto-candidates as therapeutic leads against SARS-CoV-2 infection. Environmental Challenges. 2021; 4 ():100136-100136.
Chicago/Turabian StyleChandan Dharmashekara; Sushma Pradeep; Shashanka K Prasad; Anisha S Jain; Asad Syed; Kollur Shiva Prasad; Sharanagouda S Patil; Mallikarjun S Beelagi; Chandrashekar Srinivasa; Chandan Shivamallu. 2021. "Virtual screening of potential phyto-candidates as therapeutic leads against SARS-CoV-2 infection." Environmental Challenges 4, no. : 100136-100136.
Although wheat and maize are the major economically important cereal crops and staple food sources in the world, their productivity is highly affected by excess salts in soil (salinity). Applications of multifarious halophilic plant growth-promoting rhizobacteria (PGPR) in saline soil protect the plants from osmotic damages and promote plant growth through the secretion of plant growth promoting (PGP) and osmolytes. In this study, Klebsiella variicola SURYA6—a PGPR—was evaluated for plant-growth-promotion and salinity amelioration in wheat and maize, and enrichment of soil nutrients. The results of the present study revealed that K. variicola SURYA6 grows luxuriously under high salinity stress conditions and produces copious amounts of three principal salinity ameliorating traits, such as 1 aminocyclopropane-1-carboxylate deaminase (ACCD), indole-3-acetic acid (IAA), exopolysaccharides (EPS), and osmolytes—such as proline, sugars, proteins, and amino acids. The isolate also exhibited sensitivity to a wide range of antibiotics, lack of hemolytic ability, and absence of catalase and oxidase activities confirming its nonpathogenic nature. Inoculation of wheat and maize seeds with this multifarious strain, improved the physicochemical properties of soil, improved seed germination by 33.9% and 36.0%, root length by 111.0%, 35.1%, shoot height by 64.8% and 78.9%, and chlorophyll content by 68.4% and 66.7% in wheat and maize seedlings, respectively, at 45 days after sowing (DAS) under salinity stress. The improvement in plant growth can be correlated with the secretion of PGP traits and improved, uptake of minerals such as nitrogen (N), phosphorus (P), sodium (Na), potassium (K), and magnesium (Mg). While amelioration of salinity can be the result of secretion of osmolytes and the change in pH from salinity to neutrality. This inoculation also significantly improved the soil nutrients under salinity stress conditions. Inoculation of K. variicola SURYA6, resulted in more improved growth and nutrients contents in plants and enriched soil nutrients under salinity stress as compared to normal (non-saline) conditions. Such multifarious strain can serve as a potent bio-inoculant for growth promotion of wheat and maize in saline soil. However, multi-year field trials under different agro-climatic conditions are required to confirm the bio-efficacy of K. variicola SURYA6.
Supriya Kusale; Yasmin Attar; R. Sayyed; Hesham El Enshasy; Siti Hanapi; Noshin Ilyas; Abdallah Elgorban; Ali Bahkali; Najat Marraiki. Inoculation of Klebsiella variicola Alleviated Salt Stress and Improved Growth and Nutrients in Wheat and Maize. Agronomy 2021, 11, 927 .
AMA StyleSupriya Kusale, Yasmin Attar, R. Sayyed, Hesham El Enshasy, Siti Hanapi, Noshin Ilyas, Abdallah Elgorban, Ali Bahkali, Najat Marraiki. Inoculation of Klebsiella variicola Alleviated Salt Stress and Improved Growth and Nutrients in Wheat and Maize. Agronomy. 2021; 11 (5):927.
Chicago/Turabian StyleSupriya Kusale; Yasmin Attar; R. Sayyed; Hesham El Enshasy; Siti Hanapi; Noshin Ilyas; Abdallah Elgorban; Ali Bahkali; Najat Marraiki. 2021. "Inoculation of Klebsiella variicola Alleviated Salt Stress and Improved Growth and Nutrients in Wheat and Maize." Agronomy 11, no. 5: 927.
Candida albicans, an opportunistic fungal pathogen, frequently colonizes immune-compromised patients and causes mild to severe systemic reactions. Only few antifungal drugs are currently in use for therapeutic treatment. However, evolution of a drug-resistant C. albicans fungal pathogen is of major concern in the treatment of patients, hence the clinical need for novel drug design and development. In this study, in vitro screening of novel putative pyrrolo[1,2-a]quinoline derivatives as the lead drug targets and in silico prediction of the binding potential of these lead molecules against C. albicans pathogenic proteins, such as secreted aspartic protease 3 (SAP3; 2H6T), surface protein β-glucanase (3N9K) and sterol 14-alpha demethylase (5TZ1), were carried out by molecular docking analyses. Further, biological activity-based QSAR and theoretical pharmacokinetic analysis were analyzed. Here, in vitro screening of novel analogue derivatives as drug targets against C. albicans showed inhibitory potential in the concentration of 0.4 µg for BQ-06, 07 and 08, 0.8 µg for BQ-01, 03, and 05, 1.6 µg for BQ-04 and 12.5 µg for BQ-02 in comparison to the standard antifungal drug fluconazole in the concentration of 30 µg. Further, in silico analysis of BQ-01, 03, 05 and 07 analogues docked on chimeric 2H6T, 3N9K and 5TZ1 revealed that these analogues show potential binding affinity, which is different from the therapeutic antifungal drug fluconazole. In addition, these molecules possess good drug-like properties based on the determination of conceptual Density Functional Theory (DFT)-based descriptors, QSAR and pharmacokinetics. Thus, the study offers significant insight into employing pyrrolo[1,2-a]quinoline analogues as novel antifungal agents against C. albicans that warrants further investigation.
Vijayakumar Uppar; Sandeep Chandrashekharappa; Chandan Shivamallu; Sushma P; Shiva Kollur; Joaquín Ortega-Castro; Juan Frau; Norma Flores-Holguín; Atiyaparveen Basarikatti; Mallikarjun Chougala; Mrudula Mohan M; Govindappa Banuprakash; Jayadev; Katharigatta Venugopala; Belakatte Nandeshwarappa; Ravindra Veerapur; Abdulaziz Al-Kheraif; Abdallah Elgorban; Asad Syed; Kiran Mudnakudu-Nagaraju; Basavaraj Padmashali; Daniel Glossman-Mitnik. Investigation of Antifungal Properties of Synthetic Dimethyl-4-Bromo-1-(Substituted Benzoyl) Pyrrolo[1,2-a] Quinoline-2,3-Dicarboxylates Analogues: Molecular Docking Studies and Conceptual DFT-Based Chemical Reactivity Descriptors and Pharmacokinetics Evaluation. Molecules 2021, 26, 2722 .
AMA StyleVijayakumar Uppar, Sandeep Chandrashekharappa, Chandan Shivamallu, Sushma P, Shiva Kollur, Joaquín Ortega-Castro, Juan Frau, Norma Flores-Holguín, Atiyaparveen Basarikatti, Mallikarjun Chougala, Mrudula Mohan M, Govindappa Banuprakash, Jayadev, Katharigatta Venugopala, Belakatte Nandeshwarappa, Ravindra Veerapur, Abdulaziz Al-Kheraif, Abdallah Elgorban, Asad Syed, Kiran Mudnakudu-Nagaraju, Basavaraj Padmashali, Daniel Glossman-Mitnik. Investigation of Antifungal Properties of Synthetic Dimethyl-4-Bromo-1-(Substituted Benzoyl) Pyrrolo[1,2-a] Quinoline-2,3-Dicarboxylates Analogues: Molecular Docking Studies and Conceptual DFT-Based Chemical Reactivity Descriptors and Pharmacokinetics Evaluation. Molecules. 2021; 26 (9):2722.
Chicago/Turabian StyleVijayakumar Uppar; Sandeep Chandrashekharappa; Chandan Shivamallu; Sushma P; Shiva Kollur; Joaquín Ortega-Castro; Juan Frau; Norma Flores-Holguín; Atiyaparveen Basarikatti; Mallikarjun Chougala; Mrudula Mohan M; Govindappa Banuprakash; Jayadev; Katharigatta Venugopala; Belakatte Nandeshwarappa; Ravindra Veerapur; Abdulaziz Al-Kheraif; Abdallah Elgorban; Asad Syed; Kiran Mudnakudu-Nagaraju; Basavaraj Padmashali; Daniel Glossman-Mitnik. 2021. "Investigation of Antifungal Properties of Synthetic Dimethyl-4-Bromo-1-(Substituted Benzoyl) Pyrrolo[1,2-a] Quinoline-2,3-Dicarboxylates Analogues: Molecular Docking Studies and Conceptual DFT-Based Chemical Reactivity Descriptors and Pharmacokinetics Evaluation." Molecules 26, no. 9: 2722.
Nanoworld is an attractive sphere with the potential to explore novel nanomaterials with valuable applications in medicinal science. Herein, we report an efficient and ecofriendly approach for the synthesis of Nickel oxide nanoparticles (NiO NPs) via a solution combustion method using Areca catechu leaf extract. As-prepared NiO NPs were characterized using various analytical tools such as powder X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and UV-Visible spectroscopy (UV-Vis). XRD analysis illustrates that synthesized NiO NPs are hexagonal structured crystallites with an average size of 5.46 nm and a hexagonal-shaped morphology with slight agglomeration. The morphology, size, and shape of the obtained material was further confirmed using SEM and TEM analysis. In addition, as-prepared NiO NPs have shown potential antidiabetic and anticancer properties. Our results suggest that the inhibition of α-amylase enzyme with IC 50 value 268.13 µg/mL may be one of the feasible ways through which the NiO NPs exert their hypoglycemic effect. Furthermore, cytotoxic activity performed using NiO NPs exhibited against human lung cancer cell line (A549) proved that the prepared NiO NPs have significant anticancer activity with 93.349 μg/mL at 50% inhibition concentration. The biological assay results revealed that NiO NPs exhibited significant cytotoxicity against human lung cancer cell line (A549) in a dose-dependent manner from 0–100 μg/mL, showing considerable cell viability. Further, the systematic approach deliberates the NiO NPs as a function of phenolic extracts of A. catechu with vast potential for many biological and biomedical applications.
Shwetha U R; Rajith C R; Kiran Ms; Virupaxappa Betageri; Latha M S; Ravindra Veerapur; Ghada Lamraoui; Abdulaziz Al-Kheraif; Abdallah Elgorban; Asad Syed; Chandan Shivamallu; Shiva Kollur. Biogenic Synthesis of NiO Nanoparticles Using Areca catechu Leaf Extract and Their Antidiabetic and Cytotoxic Effects. Molecules 2021, 26, 2448 .
AMA StyleShwetha U R, Rajith C R, Kiran Ms, Virupaxappa Betageri, Latha M S, Ravindra Veerapur, Ghada Lamraoui, Abdulaziz Al-Kheraif, Abdallah Elgorban, Asad Syed, Chandan Shivamallu, Shiva Kollur. Biogenic Synthesis of NiO Nanoparticles Using Areca catechu Leaf Extract and Their Antidiabetic and Cytotoxic Effects. Molecules. 2021; 26 (9):2448.
Chicago/Turabian StyleShwetha U R; Rajith C R; Kiran Ms; Virupaxappa Betageri; Latha M S; Ravindra Veerapur; Ghada Lamraoui; Abdulaziz Al-Kheraif; Abdallah Elgorban; Asad Syed; Chandan Shivamallu; Shiva Kollur. 2021. "Biogenic Synthesis of NiO Nanoparticles Using Areca catechu Leaf Extract and Their Antidiabetic and Cytotoxic Effects." Molecules 26, no. 9: 2448.
The present work describes a facile and convenient procedure for synthesizing zinc oxide nanoparticles using luteolin isolated from Eclipta alba plant (L-ZnONPs) at room temperature. The formation of as-grown L-ZnONPs was confirmed by X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), and selected area electron diffraction (SAED). The Wurtzite structure of ZnO was observed by its hexagonal phases in diffraction patterns. The SEM images revealed the different sizes and morphologies of L-ZnONPs, with diameters between 12 and 25 nm. The HR-TEM result showed that the inter-planar distance between two lattice fringes was 0.262 nm, which coincides with the d-spacing of (002) and (101) lattice planes of the as-obtained material. The anticancer activity of L-ZnONPs against the breast cancer cell line MCF-7 was greater as compared to that of luteolin or ZnO alone. The mechanistic evaluation of such an activity carried out using in silico methods suggested that the anti-breast cancer activity of L-ZnONPs was mediated by polo-like kinase 1 (PLK1) proteins.
Shiva Kollur; Shashanka Prasad; Sushma Pradeep; Ravindra Veerapur; Sharanagouda Patil; Raghavendra Amachawadi; Rajendra S; Ghada Lamraoui; Abdulaziz Al-Kheraif; Abdallah Elgorban; Asad Syed; Chandan Shivamallu. Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity. Biomolecules 2021, 11, 385 .
AMA StyleShiva Kollur, Shashanka Prasad, Sushma Pradeep, Ravindra Veerapur, Sharanagouda Patil, Raghavendra Amachawadi, Rajendra S, Ghada Lamraoui, Abdulaziz Al-Kheraif, Abdallah Elgorban, Asad Syed, Chandan Shivamallu. Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity. Biomolecules. 2021; 11 (3):385.
Chicago/Turabian StyleShiva Kollur; Shashanka Prasad; Sushma Pradeep; Ravindra Veerapur; Sharanagouda Patil; Raghavendra Amachawadi; Rajendra S; Ghada Lamraoui; Abdulaziz Al-Kheraif; Abdallah Elgorban; Asad Syed; Chandan Shivamallu. 2021. "Luteolin-Fabricated ZnO Nanostructures Showed PLK-1 Mediated Anti-Breast Cancer Activity." Biomolecules 11, no. 3: 385.
CaFe2O4 doped CdO heterojunction nanohybrid particles (CdO/CaFe2O4 NCs) was fabricated by sono-chemical assisted co-precipitation technique. X-ray powder diffraction (XRD), selected area electron diffraction (SAED) and high-resolution transmission electron microscopy (HRTEM) analysis revealed the crystallinity of CdO and CaFe2O4. Chemical states were determined using X-ray photoelectron spectroscopy (XPS). Fourier-transform infrared spectroscopy (FTIR) also indicates the successful formation of CdO/CaFe2O4 NCs. BET analyzer indicates the high surface area of NCs (44.099 m2/g) compared to individual particles. The optical, electrical and magnetic properties were studied using UV–vis diffuse reflectance spectroscopy (DRS), photoluminescence (PL) and vibrating-sample magnetometer (VSM). The resulting CdO/CaFe2O4 NCs showed enhanced performance in photocatalytic activity under visible light irradiation. The intimate NPs contact resulted in improvement in photocatalytic performance owing to the prevention of e−/h+ recombination. The h+ and OH− radicals played major role in dye degradation that was confirmed by scavengers study. The photocatalyst showed outstanding photostability and recyclability even after six photocatalytic runs. The CdO/CaFe2O4 NCs showed potential bacterial growth inhibition. The result indicates the multifunctionality of CdO/CaFe2O4 NCs that show superior photocatalytic and antimicrobial properties compared with pristine parts of CdO and CaFe2O4 NPs.
Asad Syed; Ali H. Bahkali; Abdallah M. Elgorban. Enhanced antibacterial and visible light driven photocatalytic activity of CaFe2O4 doped CdO heterojunction nanohybrid particles prepared by sono-chemical method. Optical Materials 2021, 113, 110595 .
AMA StyleAsad Syed, Ali H. Bahkali, Abdallah M. Elgorban. Enhanced antibacterial and visible light driven photocatalytic activity of CaFe2O4 doped CdO heterojunction nanohybrid particles prepared by sono-chemical method. Optical Materials. 2021; 113 ():110595.
Chicago/Turabian StyleAsad Syed; Ali H. Bahkali; Abdallah M. Elgorban. 2021. "Enhanced antibacterial and visible light driven photocatalytic activity of CaFe2O4 doped CdO heterojunction nanohybrid particles prepared by sono-chemical method." Optical Materials 113, no. : 110595.
The present study describes the biosynthesis of silver nanoparticles, using the fungus Penicillium verrucosum. The silver nanoparticles were synthesised by reacting silver nitrate (AgNO3) with the cell free filtrates of the fungal culture, and were then characterized by UV–visible spectroscopy, transmission electron microscopy, scanning electron microscopy, energy-dispersive, and X-ray diffraction analysis to further evaluate their successful biosynthesis, optical and morphological features (size and shape), and crystallinity. The bioactivity of the synthesized nanoparticles against two phytopathogenic fungi i.e: Fusarium chlamydosporum and Aspergillus flavus was evaluated using nanomaterial seeding media. These biogenic silver nanoparticles were polydisperse in nature, with a size of 10–12 nm. With regard to the antifungal activity, 150 ppm of the nanoparticles suppressed the growth of F. chlamydosporum and A. flavus by about 50%. To the best of our knowledge, this is the first report on the use of P. verrucosum to synthesise silver nanoparticles. The present study demonstrates a novel, simple, and eco-friendly process for the generation of biofunctionally useful biogenic nanoparticles.
Mohamed A. Yassin; Abdallah M. Elgorban; Abd El-Rahim M.A. El-Samawaty; Bandar M.A. Almunqedhi. Biosynthesis of silver nanoparticles using Penicillium verrucosum and analysis of their antifungal activity. Saudi Journal of Biological Sciences 2021, 28, 2123 -2127.
AMA StyleMohamed A. Yassin, Abdallah M. Elgorban, Abd El-Rahim M.A. El-Samawaty, Bandar M.A. Almunqedhi. Biosynthesis of silver nanoparticles using Penicillium verrucosum and analysis of their antifungal activity. Saudi Journal of Biological Sciences. 2021; 28 (4):2123-2127.
Chicago/Turabian StyleMohamed A. Yassin; Abdallah M. Elgorban; Abd El-Rahim M.A. El-Samawaty; Bandar M.A. Almunqedhi. 2021. "Biosynthesis of silver nanoparticles using Penicillium verrucosum and analysis of their antifungal activity." Saudi Journal of Biological Sciences 28, no. 4: 2123-2127.
The marine bacterium able to consume DDT as the nutrient source was isolated from sea water which was identified as Paracoccus sp. DDT-21 based on 16 S rDNA gene sequence and Gram negative rod, obligate aerobic, non-motile biochemical characteristics. The isolate can degrade over 80% of the DDT, at a concentration of 50 mg/L in MSM in 72 h. Time and pollutant (DDT) dependent growth studies indicated that the isolate Paracoccus sp., DDT-21 significantly degrade the DDT and tolerates under DDT stress up to 50 mg/L. The DDT degradation capability of the strain Paracoccus sp. DDT-21 was found to be 5 ˃ 10 ˃ 15 ˃ 25 ˃ 50 mg/L DDT. The high concentrations (75 and 100 mg/L) of DDT showed significant decrease in DDT degradation. The optimal DDT degradation (∼90.0%) was observed at 6 g/L of yeast extract, 6% of glucose in pH 7.0 at 35 °C with 72 h of incubation as constant. Furthermore, four metabolites were observed by GC-MS analysis such as, DDE, DDD, DDMU, and DDA. The obtained results indicate that the isolate Paracoccus sp. DDT-21 is a promising candidate for the removal and/or detoxification of DDT in the environment.
Sarah Al-Rashed; Najat Marraiki; Asad Syed; Abdallah M. Elgorban; Kollur Shiva Prasad; Chandan Shivamallu; Ali H. Bahkali. Bioremediation characteristics, influencing factors of dichlorodiphenyltrichloroethane (DDT) removal by using non-indigenous Paracoccus sp. Chemosphere 2020, 270, 129474 .
AMA StyleSarah Al-Rashed, Najat Marraiki, Asad Syed, Abdallah M. Elgorban, Kollur Shiva Prasad, Chandan Shivamallu, Ali H. Bahkali. Bioremediation characteristics, influencing factors of dichlorodiphenyltrichloroethane (DDT) removal by using non-indigenous Paracoccus sp. Chemosphere. 2020; 270 ():129474.
Chicago/Turabian StyleSarah Al-Rashed; Najat Marraiki; Asad Syed; Abdallah M. Elgorban; Kollur Shiva Prasad; Chandan Shivamallu; Ali H. Bahkali. 2020. "Bioremediation characteristics, influencing factors of dichlorodiphenyltrichloroethane (DDT) removal by using non-indigenous Paracoccus sp." Chemosphere 270, no. : 129474.
In this investigation, an alternate green-route based on myco-synthesised silver nanoparticles (Ag NPs) was evaluated to control plant disease to reduce the usage of synthetic chemicals. Here, we described biologically synthesised Ag NPs using the corn grain contaminant, Nigrospora oryzae, and were well-characterised by UV–visible spectrophotometer, X-ray powder diffraction (XRD), transmission electron microscopy (TEM), Energy Dispersive Spectroscopy (EDS) and particle size analyzer. The pathogenic behaviour of the Fusarium spp. were checked on Giza 86 and Giza 90 cultivars under greenhouse conditions. F. moniliforme and F. oxysporum exhibited high pathogenecity against Giza 90 and Giza 86 cultivars respectively. The antifungal activity of biosynthesised Ag NPs was evaluated against eight species of Fusaria causing damping-off of cotton seedlings. In vitro treatments with different concentrations of Ag NPs were achieved on Czapek Dox agar and Potato dextrose agar plates. Fungal growth was drastically retarded from 25 to 200 ppm of Ag NPs interaction. The antifungal activity of Ag NPs against the Fusarium spp. was clearly proven.
Turki M. Dawoud; Mohamed A. Yassin; Abdel Rahim M. El-Samawaty; Abdallah M. Elgorban. Silver nanoparticles synthesized by Nigrospora oryzae showed antifungal activity. Saudi Journal of Biological Sciences 2020, 28, 1847 -1852.
AMA StyleTurki M. Dawoud, Mohamed A. Yassin, Abdel Rahim M. El-Samawaty, Abdallah M. Elgorban. Silver nanoparticles synthesized by Nigrospora oryzae showed antifungal activity. Saudi Journal of Biological Sciences. 2020; 28 (3):1847-1852.
Chicago/Turabian StyleTurki M. Dawoud; Mohamed A. Yassin; Abdel Rahim M. El-Samawaty; Abdallah M. Elgorban. 2020. "Silver nanoparticles synthesized by Nigrospora oryzae showed antifungal activity." Saudi Journal of Biological Sciences 28, no. 3: 1847-1852.
Rice is a crop that is consumed as a staple food by the majority of the people in the world and therefore failure in rice crops, due to any reason, poses a severe threat of starvation. Rice blast, caused by a fungus Pyricularia oryzae, has been ranked among the most threatening plant diseases of rice and it is found wherever rice is grown. All of the rice blast disease management strategies employed so far have had limited success and rice blast has never been eliminated from rice fields. Hence, there is a need to look for the best remedy in terms of effectiveness, sustainability, and organic nature of the method. This study was aimed at determining the plant growth-promoting and fungicidal effects of a mixture of Piper caninum and Piper betle var. Nigra leaves extracts and rhizobacteria. Gas chromatography–mass spectrophotometry (GC-MS) analysis of a mixture of leaves extracts of these plants revealed the presence of new bioactive compounds such as alpha.-gurjunene, gamma.-terpinene, and ethyl 5-formyl 3-(2-ethoxycarbonyl) in a mixture of leaves extracts of P. caninum and P. betle var. Nigra. The mixture of these extracts reduced the intensity of blast disease, inhibited P. oryzae, and improved the growth, yield, and quality of Bali rice. All treatments comprising of different concentrations of a mixture of leaves extracts of P. caninum and P. betle var. Nigra plus rhizobacteria exhibited biocontrol and bioefficacy. However, a 2% concentration of a mixture of these leaves extracts with plant growth-promoting rhizobacteria (PGPR) exhibited potent inhibition of growth of P. oryzae, a significant reduction in the intensity of blast disease, and a maximum increase in growth, yield, and quality of Bali rice. In the 15th week, the intensity of blast disease decreased from 80.18% to 7.90%. The mixture of leaves extract + PGPR also improved the height of the plant, the number of tillers, number of leaves, number of grains per panicle, number of heads per panicle, and the full-grain weight per clump. Applications of various concentrations of a mixture of leaves extracts + PGPR resulted in improvement in the potential yield of rice, however, the application of 2% extracts + PGPR gave the highest potential yield of 5.61 tha−1 compared to the low yields in the control and other treatments. The high grain yield observed with the treatment was caused by the low intensity of blast disease. This treatment also strengthened the stem and prevented the drooping of the plant and improved the quality of rice grain.
Ni Luh Suriani; Dewa Ngurah Suprapta; Novizar Nazir; Ni Made Susun Parwanayoni; Anak Agung Ketut Darmadi; Desy Andya Dewi; Ni Sudatri; Ahmad Fudholi; R. Sayyed; Asad Syed; Abdallah Elgorban; Ali Bahkali; Hesham El Enshasy; Daniel Dailin. A Mixture of Piper Leaves Extracts and Rhizobacteria for Sustainable Plant Growth Promotion and Bio-Control of Blast Pathogen of Organic Bali Rice. Sustainability 2020, 12, 8490 .
AMA StyleNi Luh Suriani, Dewa Ngurah Suprapta, Novizar Nazir, Ni Made Susun Parwanayoni, Anak Agung Ketut Darmadi, Desy Andya Dewi, Ni Sudatri, Ahmad Fudholi, R. Sayyed, Asad Syed, Abdallah Elgorban, Ali Bahkali, Hesham El Enshasy, Daniel Dailin. A Mixture of Piper Leaves Extracts and Rhizobacteria for Sustainable Plant Growth Promotion and Bio-Control of Blast Pathogen of Organic Bali Rice. Sustainability. 2020; 12 (20):8490.
Chicago/Turabian StyleNi Luh Suriani; Dewa Ngurah Suprapta; Novizar Nazir; Ni Made Susun Parwanayoni; Anak Agung Ketut Darmadi; Desy Andya Dewi; Ni Sudatri; Ahmad Fudholi; R. Sayyed; Asad Syed; Abdallah Elgorban; Ali Bahkali; Hesham El Enshasy; Daniel Dailin. 2020. "A Mixture of Piper Leaves Extracts and Rhizobacteria for Sustainable Plant Growth Promotion and Bio-Control of Blast Pathogen of Organic Bali Rice." Sustainability 12, no. 20: 8490.
Diaporthe taiwanensis sp. nov. (Diaporthales, Sordariomycetes, Ascomycota) isolated on living leaves of Ixora chinensis from Taiwan, is characterized using both phenotypic and genetic characters. Phylogenetic reconstructions based on concatenated DNA sequence data of four nuclear genetic markers (ITS, tef1-α, β-tubulin and cal) show that the new taxon is separated from other representative species of Diaporthe with high statistical support. The new taxon differs from its phylogenetically related congeners mainly by having larger alpha conidia and producing beta conidia in culture and further by host and geographical distribution. Artificial inoculation on leaves confirmed the pathogenicity of the novel fungus to Ixora. The present study is the first report of Diaporthe species causing leaf spots and necrosis on Ixora chinensis in Taiwan.
Hiran A. Ariyawansa; Ichen Tsai; Patchareeya Withee; Medsaii Tanjira; Chia-Yun Yen; Sarah Al-Rashed; Abdallah M. Elgorban; Ratchadawan Cheewangkoon. Diaporthe taiwanensis: A new taxon causing leaf spots and necrosis on Ixora chinensis in Taiwan. Phytotaxa 2020, 461, 155 -165.
AMA StyleHiran A. Ariyawansa, Ichen Tsai, Patchareeya Withee, Medsaii Tanjira, Chia-Yun Yen, Sarah Al-Rashed, Abdallah M. Elgorban, Ratchadawan Cheewangkoon. Diaporthe taiwanensis: A new taxon causing leaf spots and necrosis on Ixora chinensis in Taiwan. Phytotaxa. 2020; 461 (3):155-165.
Chicago/Turabian StyleHiran A. Ariyawansa; Ichen Tsai; Patchareeya Withee; Medsaii Tanjira; Chia-Yun Yen; Sarah Al-Rashed; Abdallah M. Elgorban; Ratchadawan Cheewangkoon. 2020. "Diaporthe taiwanensis: A new taxon causing leaf spots and necrosis on Ixora chinensis in Taiwan." Phytotaxa 461, no. 3: 155-165.