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Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV), referred to as small ruminant lentiviruses (SRLVs), belong to the genus Lentivirus of the Retroviridae family. SRLVs infect both sheep and goats, causing significant economic losses and animal welfare damage. Recent findings suggest an association between serological status and allelic variants of different genes such as TMEM154, TLR9, MYD88 and CCR5. The aim of this work was to investigate the role of specific polymorphisms of these genes in SRLVs infection in some sheep flocks in Italy. In addition to those already known, novel variants in the TMEM154 (P7H, I74V, I105V) gene were detected in this study. The risk of infection was determined finding an association between the serological status and polymorphisms P7H, E35K, N70I, I74V, I105V of TMEM154, R447Q, A462S and G520R in TLR9 gene, H176H* and K190K* in MYD88 genes, while no statistical association was observed for the 4-bp deletion of the CCR5 gene. Since no vaccines or treatments have been developed, a genetically based approach could be an innovative strategy to prevent and to control SRLVs infection. Our findings are an important starting point in order to define the genetic resistance profile towards SRLVs infection.
Chiara Arcangeli; Daniele Lucarelli; Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Claudia Pellegrini; Andrea Felici; Silva Costarelli; Monica Giammarioli; Francesco Feliziani; Fabrizio Passamonti; Massimo Biagetti. First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection. Viruses 2021, 13, 1290 .
AMA StyleChiara Arcangeli, Daniele Lucarelli, Martina Torricelli, Carla Sebastiani, Marcella Ciullo, Claudia Pellegrini, Andrea Felici, Silva Costarelli, Monica Giammarioli, Francesco Feliziani, Fabrizio Passamonti, Massimo Biagetti. First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection. Viruses. 2021; 13 (7):1290.
Chicago/Turabian StyleChiara Arcangeli; Daniele Lucarelli; Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Claudia Pellegrini; Andrea Felici; Silva Costarelli; Monica Giammarioli; Francesco Feliziani; Fabrizio Passamonti; Massimo Biagetti. 2021. "First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection." Viruses 13, no. 7: 1290.
In goats, as in sheep, genotypes of the prion protein gene (PRNP) can influence animals’ susceptibility to scrapie. Since the polymorphic codons in sheep are well known, a genetic selection plan has been implemented in Europe, in order to reduce the prevalence of susceptible genotypes to scrapie. In Italy, no breeding plan for scrapie resistance in goats has been adopted, yet. Likewise, according to the most recent modification of Regulation EU 999/2001 (Regulation EU 772/2020) of the European Commission (EU), based on all the available experimental and in field data, K222, D146 and S146 polymorphisms could be used as scrapie resistance alleles in genetic management both in scrapie outbreaks and in disease prevention. In order to collect data on the variability of PRNP, the present study aimed to analyze the sequence of the PRNP gene in eight Italian local goat populations/breeds reared in central and southern Italy (Bianca Monticellana, Capestrina, Facciuta della Valnerina, Fulva del Lazio, Garganica, Grigia Ciociara, Grigia Molisana, and Teramana), some of which were investigated for the first time; moreover, two cosmopolitan breeds (Alpine and Saanen) were included. Blood samples were collected from 219 goats. Genomic DNA was extracted from whole blood. DNA was used as template in PCR amplification of the entire PRNP open reading frame (ORF). Purified amplicons have been sequenced and aligned to Capra hircus PRNP. Particularly, the alleles carrying the resistance-related 222 K polymorphism occurred in all populations with a frequency between 2.5% and 12.5%. An additional resistance allele carrying the S146 variant was observed with a frequency of 3.7% only in the Alpine breed. For three of the estimated alleles, we could not establish if the found double polymorphisms in heterozygosis were in phase, due to technical limitations. In this context, in addition to selective culling in scrapie outbreaks according to the European regulation in force, in the future, selection plans could be adopted to deal with scrapie and to control its diffusion, meanwhile paying attention to preserve a high variability of PRNP.
Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Simone Ceccobelli; Barbara Chiappini; Gabriele Vaccari; Antonio Capocefalo; Michela Conte; Samira Giovannini; Emiliano Lasagna; Francesca Sarti; Massimo Biagetti. PRNP Polymorphisms in Eight Local Goat Populations/Breeds from Central and Southern Italy. Animals 2021, 11, 333 .
AMA StyleMartina Torricelli, Carla Sebastiani, Marcella Ciullo, Simone Ceccobelli, Barbara Chiappini, Gabriele Vaccari, Antonio Capocefalo, Michela Conte, Samira Giovannini, Emiliano Lasagna, Francesca Sarti, Massimo Biagetti. PRNP Polymorphisms in Eight Local Goat Populations/Breeds from Central and Southern Italy. Animals. 2021; 11 (2):333.
Chicago/Turabian StyleMartina Torricelli; Carla Sebastiani; Marcella Ciullo; Simone Ceccobelli; Barbara Chiappini; Gabriele Vaccari; Antonio Capocefalo; Michela Conte; Samira Giovannini; Emiliano Lasagna; Francesca Sarti; Massimo Biagetti. 2021. "PRNP Polymorphisms in Eight Local Goat Populations/Breeds from Central and Southern Italy." Animals 11, no. 2: 333.
A 40 years old male Nile crocodile (Crocodylus niloticus) was diagnosed with pulmonary mycobacteriosis caused by a member of Mycobacterium chelonae/abscessus group. Post-mortem examination showed a severe systemic visceral granulomatous involvement, with lesions in lungs, heart, liver, spleen and kidneys. Histopathological examination of lung, spleen, heart and liver revealed multifocal to coalescing granulomas showing eterophils in central zone and outer rim of epithelioid histiocytes, multinucleated giant cells and lymphocytes. The Ziehl–Neelsen histological staining revealed rare vacuoles containing numerous alcohol-acid resistant bacteria. Mycobacterial infection was confirmed by culture and PCR targeting rRNA 16S gene. Sequence analysis of the DNA amplicon revealed a 100% homology with the M. chelonae/ abscessus group. Even if the classification of the memebrr of this group is still on updating, to the best of our knowledge, this is the first report of M. chelonae/abscessus member infection in a Nile crocodile species.
Marco Gobbi; Sara Corneli; Nicoletta D'avino; Elisabetta Manuali; Antonella Di Paolo; Carla Sebastiani; Marcella Ciullo; Michele Tentellini; Maria Lodovica Pacciarini; Martina Sebastianelli; Silvia Pavone; Piera Mazzone. Granulomatous Pneumonia in a Nile Crocodile (Crocodylus niloticus) Caused by a Member of Mycobacterium Chelonae/Abscessus Group. 2020, 1 .
AMA StyleMarco Gobbi, Sara Corneli, Nicoletta D'avino, Elisabetta Manuali, Antonella Di Paolo, Carla Sebastiani, Marcella Ciullo, Michele Tentellini, Maria Lodovica Pacciarini, Martina Sebastianelli, Silvia Pavone, Piera Mazzone. Granulomatous Pneumonia in a Nile Crocodile (Crocodylus niloticus) Caused by a Member of Mycobacterium Chelonae/Abscessus Group. . 2020; ():1.
Chicago/Turabian StyleMarco Gobbi; Sara Corneli; Nicoletta D'avino; Elisabetta Manuali; Antonella Di Paolo; Carla Sebastiani; Marcella Ciullo; Michele Tentellini; Maria Lodovica Pacciarini; Martina Sebastianelli; Silvia Pavone; Piera Mazzone. 2020. "Granulomatous Pneumonia in a Nile Crocodile (Crocodylus niloticus) Caused by a Member of Mycobacterium Chelonae/Abscessus Group." , no. : 1.
Bovine milk contains several β-casein variants, with the A1 and A2 variants occurring most frequently. The presence of some variants, such as A1, B, and C, is considered a risk factor for disease in humans who consume milk. These variants are probably involved in intolerance to milk and some human diseases due to the production of a bioactive peptide with opioid activity during digestion, β-casomorphin 7 (BCM-7). In contrast, the A2 variant is not involved in pathogenetic mechanisms; thus, its presence in milk is a desirable feature. The difference between the A1 and A2 variants is a mutation at position 67 of the β-casein gene (CSN2), which causes an amino acid to change from histidine (in the A1, B, and C variants) to proline (in the A2 variant). To select dairy cows on the basis of the presence of the β-casein variant A2, allele frequencies of CSN2 variants were evaluated in Italian dairy cows reared in central Italy. The results of this study may help with the selection of animals with the β-casein gene variant A2 to produce a more digestible milk that only contains the β-casein variant A2. The majority of proteins in cow’s milk are caseins, which occur in four groups (α-s1, α-s2, β, and k) encoded by different genes (CSN1S1, CSN1S2, CSN2, and CSN3, respectively). In this study, we focused on the β-casein allele variants A1 and A2 due to their influence on milk’s technological characteristics and human health. Digestion of the β-casein variant A1 leads to the formation of β-casomorphin 7 (BCM-7), a bioactive peptide that has been suggested to be a possible cause of various human diseases and associated with low milk digestibility. The potential negative role of the β-casein variant A1 in human health has stimulated the planning of cattle breeding programs based on genetic selection to increase the frequency of the A2 variant, which is associated with increased milk digestibility. The aim of this work was to evaluate the frequencies of the different β-casein variants in Italian Holstein Friesian dairy cows from cattle farms located in central Italy to select a population of A2 homozygous animals. β-casein genotypes were identified by evaluating the presence of single nucleotide polymorphisms (SNPs) of the CSN2 gene using PCR and sequencing analysis. The frequency of the desirable β-casein variant A2 in the studied bovine population was 0.61. The frequency of the undesirable A1 variant in the studied bovine population was 0.30. The frequency of the A2 allele was higher than expected for the breed; therefore, genetic selection for the A2 variant in these animals could be achieved in a fairly short time using A2 homozygous bulls.
Carla Sebastiani; Chiara Arcangeli; Marcella Ciullo; Martina Torricelli; Giulia Cinti; Stefano Fisichella; Massimo Biagetti. Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals 2020, 10, 252 .
AMA StyleCarla Sebastiani, Chiara Arcangeli, Marcella Ciullo, Martina Torricelli, Giulia Cinti, Stefano Fisichella, Massimo Biagetti. Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals. 2020; 10 (2):252.
Chicago/Turabian StyleCarla Sebastiani; Chiara Arcangeli; Marcella Ciullo; Martina Torricelli; Giulia Cinti; Stefano Fisichella; Massimo Biagetti. 2020. "Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy." Animals 10, no. 2: 252.
African swine fever (ASF) virus is a DNA virus responsible for a severe haemorrhagic fever in pigs, which (still in the absence of vaccination strategies) results in high mortality rates. Herein, we present a biosensor-based method for the detection of ASF viral DNA in the blood of pigs. The biosensor exploits a single-strand DNA probe with locked nucleic acid nucleotides (LNA) substitutions as the complementary recognition element for the conserved region of vp72 gene of ASF virus. The biosensor was calibrated using qPCR-quantified ASF viral DNA extracted from the blood of pigs experimentally infected with the virulent Italian isolate 49/08, genotype I. Globally, the proposed biosensor showed good sensitivity and specificity, with the limits of detection (LOD) and quantification (LOQ) being 178 and 245 copies/μL of genomic ASF viral DNA, respectively. The reversible nature of the interaction between the DNA/LNA probe and the target DNA sequence granted multiple rapid analyses, with up to 40 analyses per single surface possible, and a single test requiring approximately 5 min. When applied to non-amplified DNA extracts from the blood of field-infected pigs, the assay discriminated between ASFV-infected and ASFV non-infected animals, and allowed the rapid quantification of ASF viral DNA, with values falling in the range 373–1058 copies/μL of genomic ASFV DNA. In this range, excellent correlation was observed between the results of this biosensor and OIE-approved qPCR. This method represents a promising screening assay for preliminary ASF diagnosis, having the major advantages in the relative rapidity, ease-of-use, the reusability of the sensing surface, and low cost per single test.
Massimo Biagetti; Massimiliano Cuccioloni; Laura Bonfili; Valentina Cecarini; Carla Sebastiani; Ludovica Curcio; Monica Giammarioli; Gian Mario De Mia; Anna Maria Eleuteri; Mauro Angeletti. Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus. Talanta 2018, 184, 35 -41.
AMA StyleMassimo Biagetti, Massimiliano Cuccioloni, Laura Bonfili, Valentina Cecarini, Carla Sebastiani, Ludovica Curcio, Monica Giammarioli, Gian Mario De Mia, Anna Maria Eleuteri, Mauro Angeletti. Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus. Talanta. 2018; 184 ():35-41.
Chicago/Turabian StyleMassimo Biagetti; Massimiliano Cuccioloni; Laura Bonfili; Valentina Cecarini; Carla Sebastiani; Ludovica Curcio; Monica Giammarioli; Gian Mario De Mia; Anna Maria Eleuteri; Mauro Angeletti. 2018. "Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus." Talanta 184, no. : 35-41.
Abortion in ruminants represents an important economic concern for farmers. Microbial agents, such as Brucella spp., Chlamydia spp., Coxiella burnetii, Leptospira spp., Neospora caninum, Salmonella spp. and Toxoplasma gondii, are among the main infectious causes of abortion and require rapid and reliable diagnosis. This study describes the development of a multi-screening assay using Fast Real-Time PCR (Fast qPCR) that allows, in a single test, the simultaneous identification of the above-mentioned abortive agents. This multi-screening approach is characterized by a mean diagnostic sensitivity and specificity of 100% and 97%, respectively; it has a limit of detection (LOD) ranging from 5 × 103 to 4 × 104 genomic copies/g of tissue and a very good concordance with traditional end-point PCR assays used in routine diagnostic activity. The proposed method represents a rapid approach to the simultaneous detection of the main abortive agents in ruminants that allows to make an accurate diagnosis and to set up appropriate control measures in a short period of time.
Carla Sebastiani; Ludovica Curcio; Marcella Ciullo; Deborah Cruciani; Silvia Crotti; Cristina Pesca; Martina Torricelli; Martina Sebastianelli; Andrea Felici; Massimo Biagetti. A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants. Journal of Microbiological Methods 2018, 148, 12 -17.
AMA StyleCarla Sebastiani, Ludovica Curcio, Marcella Ciullo, Deborah Cruciani, Silvia Crotti, Cristina Pesca, Martina Torricelli, Martina Sebastianelli, Andrea Felici, Massimo Biagetti. A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants. Journal of Microbiological Methods. 2018; 148 ():12-17.
Chicago/Turabian StyleCarla Sebastiani; Ludovica Curcio; Marcella Ciullo; Deborah Cruciani; Silvia Crotti; Cristina Pesca; Martina Torricelli; Martina Sebastianelli; Andrea Felici; Massimo Biagetti. 2018. "A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants." Journal of Microbiological Methods 148, no. : 12-17.
Aims To evaluate the survival of Mycobacterium avium subsp. paratuberculosis (MAP) during anaerobic digestion (AD), we studied two different biogas plants loaded with manure and slurry from paratuberculosis‐infected dairy herds. Methods and Results Plants operated under mesophilic conditions, the first with a single digester and the second with a double digester. MAP detection was performed by sampling each stage of the process, specifically the pre‐fermenter, fermenter, liquid digestate and solid digestate stages, for 11 months. In both plants, MAP was isolated from the pre‐fermenter stage. Only the final products, the solid and liquid digestates, of the one‐stage plant showed viable MAP, while no viable MAP was detected in the digestates of the two‐stage plant. Conclusions MAP showed a significant decrease during subsequent steps of the AD process, particularly in the two‐stage plant. We suggest that the second digester maintained the digestate under anaerobic conditions for a longer period of time, thus reducing MAP survival and MAP load under the culture detection limit. Significance and Impact of the Study Our data are unable to exclude the presence of MAP in the final products of the biogas plants, particularly those products from the single digester; therefore, the use of digestates as fertilizers is a real concern related to the possible environmental contamination with MAP. This article is protected by copyright. All rights reserved.
P. Mazzone; S. Corneli; A. Di Paolo; C. Maresca; A. Felici; M. Biagetti; M. Ciullo; Carla Sebastiani; G. Pezzotti; Simone Leo; M. Ricchi; N. Arrigoni. Survival of Mycobacterium avium subsp. paratuberculosis in the intermediate and final digestion products of biogas plants. Journal of Applied Microbiology 2018, 125, 36 -44.
AMA StyleP. Mazzone, S. Corneli, A. Di Paolo, C. Maresca, A. Felici, M. Biagetti, M. Ciullo, Carla Sebastiani, G. Pezzotti, Simone Leo, M. Ricchi, N. Arrigoni. Survival of Mycobacterium avium subsp. paratuberculosis in the intermediate and final digestion products of biogas plants. Journal of Applied Microbiology. 2018; 125 (1):36-44.
Chicago/Turabian StyleP. Mazzone; S. Corneli; A. Di Paolo; C. Maresca; A. Felici; M. Biagetti; M. Ciullo; Carla Sebastiani; G. Pezzotti; Simone Leo; M. Ricchi; N. Arrigoni. 2018. "Survival of Mycobacterium avium subsp. paratuberculosis in the intermediate and final digestion products of biogas plants." Journal of Applied Microbiology 125, no. 1: 36-44.
Lucilla Cucco; Francesca Romana Massacci; Carla Sebastiani; Piermario Mangili; Luca Bano; Monia Cocchi; Andrea Luppi; Roberta Ortenzi; Giovanni Pezzotti; Chiara Francesca Magistrali. Molecular characterization and antimicrobial susceptibility of Pasteurella multocida strains isolated from hosts affected by various diseases in Italy. Vet. Ital. 2017, 53, 21 -27.
AMA StyleLucilla Cucco, Francesca Romana Massacci, Carla Sebastiani, Piermario Mangili, Luca Bano, Monia Cocchi, Andrea Luppi, Roberta Ortenzi, Giovanni Pezzotti, Chiara Francesca Magistrali. Molecular characterization and antimicrobial susceptibility of Pasteurella multocida strains isolated from hosts affected by various diseases in Italy. Vet. Ital.. 2017; 53 (1):21-27.
Chicago/Turabian StyleLucilla Cucco; Francesca Romana Massacci; Carla Sebastiani; Piermario Mangili; Luca Bano; Monia Cocchi; Andrea Luppi; Roberta Ortenzi; Giovanni Pezzotti; Chiara Francesca Magistrali. 2017. "Molecular characterization and antimicrobial susceptibility of Pasteurella multocida strains isolated from hosts affected by various diseases in Italy." Vet. Ital. 53, no. 1: 21-27.
Scrapie is a naturally occurring transmissible spongiform encephalopathy in sheep and goat. It has been known for ~250 years and is characterised by the accumulation of an abnormal isoform of a host-encoded prion protein that leads to progressive neurodegeneration and death. Scrapie is recognised in two forms, classical and atypical scrapie. The susceptibility to both types of scrapie is influenced by polymorphisms of the prion protein gene (PRNP). Sheep susceptibility or resistance to classical scrapie is strongly regulated by the polymorphisms at codons 136, 154 and 171 of the PRNP. The genetic role in atypical scrapie in sheep has been defined by polymorphisms at codons 141, 154 and 171, which are associated with different degrees of risk in the occurrence of the ovine disease. Progress has been achieved in the prevention of scrapie in sheep due to efficient genetic breeding programmes based on eradication and control of the disease. In Europe, the success of these programmes has been verified by applying eradication and genetic selection plans. In general terms, the ovine selection plans aim to eliminate and reduce the susceptible allele and to enrich the resistant allele ARR. During outbreaks all susceptible animals are slaughtered, only ARR/ARR resistant rams and sheep and semi-resistant females are preserved. In the occurrence of scrapie positive goats a complete cull of the flock (stamping out) is performed with great economic loss and severe risk of extinction for the endangered breeds. The ability to select scrapie-resistant animals allows to define new breeding strategies aimed to boost genetic progress while reducing costs during scrapie outbreaks. Allelic variants of PRNP can be protective for caprine scrapie, and the knowledge of their distribution in goats has become very important. Over the past few years, the integration of genetic information on goat populations could be used to make selection decisions, commonly referred to as genetic selection. The objective of this review was to summarise the main findings of polymorphisms of the caprine prion protein (PrP) gene and to discuss the possible application of goat breeding schemes integrating genetic selection, with their relative advantages and limitations.
L. Curcio; Carla Sebastiani; P. Di Lorenzo; E. Lasagna; Massimo Biagetti. Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease. Animal 2016, 10, 1585 -1593.
AMA StyleL. Curcio, Carla Sebastiani, P. Di Lorenzo, E. Lasagna, Massimo Biagetti. Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease. Animal. 2016; 10 (10):1585-1593.
Chicago/Turabian StyleL. Curcio; Carla Sebastiani; P. Di Lorenzo; E. Lasagna; Massimo Biagetti. 2016. "Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease." Animal 10, no. 10: 1585-1593.
Ludovica Curcio; Carla Sebastiani; Simone Ceccobelli; Gabriele Vaccari; Giovanni Pezzotti; Emiliano Lasagna; Massimo Biagetti. PRNP polymorphisms in four Italian sheep breeds. Livestock Science 2015, 181, 38 -42.
AMA StyleLudovica Curcio, Carla Sebastiani, Simone Ceccobelli, Gabriele Vaccari, Giovanni Pezzotti, Emiliano Lasagna, Massimo Biagetti. PRNP polymorphisms in four Italian sheep breeds. Livestock Science. 2015; 181 ():38-42.
Chicago/Turabian StyleLudovica Curcio; Carla Sebastiani; Simone Ceccobelli; Gabriele Vaccari; Giovanni Pezzotti; Emiliano Lasagna; Massimo Biagetti. 2015. "PRNP polymorphisms in four Italian sheep breeds." Livestock Science 181, no. : 38-42.
Yersinia pseudotuberculosis is a pathogen that infects both animals and humans worldwide. The epidemiology of infection caused by Y. pseudotuberculosis is poorly understood; however, its outbreaks have been traced back to a probable source in wildlife. This study aimed to characterise Y. pseudotuberculosis isolates collected from animals with yersiniosis. This study included 90 isolates of Y. pseudotuberculosis collected from different animals with yersiniosis between 1996 and 2013 in Italy. The isolates were tested for antimicrobial susceptibility and were biotyped. Genes associated with virulence plasmid pYV and those encoding O-antigen, high pathogenicity island (HPI), and superantigenic toxin (YPM) were determined by performing PCR. Pulsed-field gel electrophoresis (PFGE) was performed using NotI and SpeI enzymes, and 3 dendrograms were generated. No antibiotic resistance was found. The presence of pYV was shown in 57 out of 90 isolates. Virulence profiles of majority of the isolates indicated that they belonged to O:1a and O:1b serotypes, biotype 1, and genetic type 2. Isolates belonging to O:2a serotype were detected in sheep and cattle and were found to be associated (for the first time) with septicemia in hares. Y. pseudotuberculosis isolates belonging to O:5a and O:12-O13 serotypes were also detected in hares. To our knowledge, this is the first study to detect Y. pseudotuberculosis isolates belonging to the O:12-O13 serotype from a clinical case in Europe. Results of PFGE indicated that it was a reliable method for investigating the genetic relatedness of Y. pseudotuberculosis isolates. Thus, characterisation of Y. pseudotuberculosis infection in animals should be considered a possible tool for the surveillance of pseudotuberculosis.
C.F. Magistrali; L. Cucco; Giovanni Pezzotti; S. Farneti; V. Cambiotti; S. Catania; P. Prati; M. Fabbi; Stefano Antonio Lollai; P. Mangili; Carla Sebastiani; L. Bano; Anna Maria Dionisi; I. Luzzi. Characterisation of Yersinia pseudotuberculosis isolated from animals with yersiniosis during 1996–2013 indicates the presence of pathogenic and Far Eastern strains in Italy. Veterinary Microbiology 2015, 180, 161 -166.
AMA StyleC.F. Magistrali, L. Cucco, Giovanni Pezzotti, S. Farneti, V. Cambiotti, S. Catania, P. Prati, M. Fabbi, Stefano Antonio Lollai, P. Mangili, Carla Sebastiani, L. Bano, Anna Maria Dionisi, I. Luzzi. Characterisation of Yersinia pseudotuberculosis isolated from animals with yersiniosis during 1996–2013 indicates the presence of pathogenic and Far Eastern strains in Italy. Veterinary Microbiology. 2015; 180 (1-2):161-166.
Chicago/Turabian StyleC.F. Magistrali; L. Cucco; Giovanni Pezzotti; S. Farneti; V. Cambiotti; S. Catania; P. Prati; M. Fabbi; Stefano Antonio Lollai; P. Mangili; Carla Sebastiani; L. Bano; Anna Maria Dionisi; I. Luzzi. 2015. "Characterisation of Yersinia pseudotuberculosis isolated from animals with yersiniosis during 1996–2013 indicates the presence of pathogenic and Far Eastern strains in Italy." Veterinary Microbiology 180, no. 1-2: 161-166.
Ludovica Curcio; Emiliano Lasagna; Francesca Maria Sarti; Carla Sebastiani; Giovanni Pezzotti; Massimo Biagetti. Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed. Italian Journal of Animal Science 2015, 14, 4251 .
AMA StyleLudovica Curcio, Emiliano Lasagna, Francesca Maria Sarti, Carla Sebastiani, Giovanni Pezzotti, Massimo Biagetti. Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed. Italian Journal of Animal Science. 2015; 14 (4):4251.
Chicago/Turabian StyleLudovica Curcio; Emiliano Lasagna; Francesca Maria Sarti; Carla Sebastiani; Giovanni Pezzotti; Massimo Biagetti. 2015. "Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed." Italian Journal of Animal Science 14, no. 4: 4251.
Atypical Yersinia pseudotuberculosis serotype O:3 was isolated from rectal contents of two wild boars hunted in Italy within a regional wildlife management program. No outbreak of yersiniosis was reported in this area in the same period and no lesions were found by the veterinarian at post-mortem inspection. Nevertheless, after histological examination, granulomatous lesions were detected in submandibular lymph nodes of one of the two wild boars. Microbiological and bio molecular characterization of the isolates revealed a melibiose-negative, biotype 2, wbyK+O:3 genotype, carrying inv, yop (yopH and yopB), virF, and R-HPI. Strains showing the same profile, matching to the criteria of genetic group 5, have been recently reported in fatal cases of yersiniosis in cynomolgus macaques and in farmed deer and atypical O:3 serotype has been suggested as a pathogenic subtype of O:3. This is the third report of an atypical O:3 Y. pseudotuberculosis strain, the first outside the American continent and the first one not associated to fatal yersiniosis. Wild boars could be a possible reservoir of this emerging pathogen.
C.F. Magistrali; L. Cucco; E. Manuali; Carla Sebastiani; S. Farneti; L. Ercoli; Giovanni Pezzotti. Atypical Yersinia pseudotuberculosis serotype O:3 isolated from hunted wild boars in Italy. Veterinary Microbiology 2014, 171, 227 -231.
AMA StyleC.F. Magistrali, L. Cucco, E. Manuali, Carla Sebastiani, S. Farneti, L. Ercoli, Giovanni Pezzotti. Atypical Yersinia pseudotuberculosis serotype O:3 isolated from hunted wild boars in Italy. Veterinary Microbiology. 2014; 171 (1-2):227-231.
Chicago/Turabian StyleC.F. Magistrali; L. Cucco; E. Manuali; Carla Sebastiani; S. Farneti; L. Ercoli; Giovanni Pezzotti. 2014. "Atypical Yersinia pseudotuberculosis serotype O:3 isolated from hunted wild boars in Italy." Veterinary Microbiology 171, no. 1-2: 227-231.
Fabio Macchioni; Franco Cecchi; Roberta Ciampolini; Massimo Biagetti; Elena Ciani; G Filippini; P Papa; Carla Sebastiani; D Cianci. The genetic resistance to gastro-intestinal strongylids in Appenninica sheep: preliminary results. Parassitologia 2007, 49, 1 .
AMA StyleFabio Macchioni, Franco Cecchi, Roberta Ciampolini, Massimo Biagetti, Elena Ciani, G Filippini, P Papa, Carla Sebastiani, D Cianci. The genetic resistance to gastro-intestinal strongylids in Appenninica sheep: preliminary results. Parassitologia. 2007; 49 (1):1.
Chicago/Turabian StyleFabio Macchioni; Franco Cecchi; Roberta Ciampolini; Massimo Biagetti; Elena Ciani; G Filippini; P Papa; Carla Sebastiani; D Cianci. 2007. "The genetic resistance to gastro-intestinal strongylids in Appenninica sheep: preliminary results." Parassitologia 49, no. 1: 1.
G. Filippini; V. Cetica; Roberta Ciampolini; M. Biagetti; Francesca Cecchi; E. Mazzanti; Elena Ciani; Carla Sebastiani; G. Venditti. Beef Traceability Using Molecular Methodologies. Veterinary Research Communications 2006, 30, 375 -377.
AMA StyleG. Filippini, V. Cetica, Roberta Ciampolini, M. Biagetti, Francesca Cecchi, E. Mazzanti, Elena Ciani, Carla Sebastiani, G. Venditti. Beef Traceability Using Molecular Methodologies. Veterinary Research Communications. 2006; 30 (1):375-377.
Chicago/Turabian StyleG. Filippini; V. Cetica; Roberta Ciampolini; M. Biagetti; Francesca Cecchi; E. Mazzanti; Elena Ciani; Carla Sebastiani; G. Venditti. 2006. "Beef Traceability Using Molecular Methodologies." Veterinary Research Communications 30, no. 1: 375-377.
Assignment tests based on multilocus genotypes are becoming increasingly important to certify quality and origin of livestock products and assure food safety and authenticity. The purpose of this study was to determine the potential of microsatellites (STR) for determining the breed origin of beef products among cattle breeds present in the market. We typed 19 STR in 269 animals from 4 cattle breeds. Based on Wright's F-statistics, 4 loci were discarded, and the remaining 15 loci (FIT = 0.101, FST = 0.089, and FIS = 0.013) were used to compute the likelihood that each multilocus genotype of the total sample was drawn from its true breed instead of another breed. To avoid occurrence of zero likelihood when one or more alleles were missing from a tested breed, sample allele frequencies were estimated assuming uniform prior distributions. Log-likelihood ratio [log(LR)] distributions of the individual assignments were determined for all possible breed contrasts, and their means and SD were used to infer the true-positive and false-positive rates at several values of the log(LR). The posterior probability that the animals of a presumed breed were actually drawn from that breed instead of any another breed was then calculated. Given an observed value of log(LR) > 0 and assuming equal priors, these probabilities were >99.5% in 10 of 12 possible breed contrasts. For the 2 most closely related breeds (FST = 0.041), this probability was 96.3%, and the probability of excluding the origin of an animal from an alleged breed when it was actually derived from another breed was similar.
Roberta Ciampolini; V. Cetica; Elena Ciani; E. Mazzanti; X. Fosella; F. Marroni; M. Biagetti; Carla Sebastiani; P. Papa; G. Filippini; D. Cianci; S. Presciuttini. Statistical analysis of individual assignment tests among four cattle breeds using fifteen STR loci1. Journal of Animal Science 2006, 84, 11 -19.
AMA StyleRoberta Ciampolini, V. Cetica, Elena Ciani, E. Mazzanti, X. Fosella, F. Marroni, M. Biagetti, Carla Sebastiani, P. Papa, G. Filippini, D. Cianci, S. Presciuttini. Statistical analysis of individual assignment tests among four cattle breeds using fifteen STR loci1. Journal of Animal Science. 2006; 84 (1):11-19.
Chicago/Turabian StyleRoberta Ciampolini; V. Cetica; Elena Ciani; E. Mazzanti; X. Fosella; F. Marroni; M. Biagetti; Carla Sebastiani; P. Papa; G. Filippini; D. Cianci; S. Presciuttini. 2006. "Statistical analysis of individual assignment tests among four cattle breeds using fifteen STR loci1." Journal of Animal Science 84, no. 1: 11-19.
Acute myelogenous leukemias (AMLs) are genetically heterogeneous and characterized by chromosomal rearrangements that produce fusion proteins with aberrant transcriptional regulatory activities. Expression of AML fusion proteins in transgenic mice increases the risk of myeloid leukemias, suggesting that they induce a preleukemic state. The underlying molecular and biological mechanisms are, however, unknown. To address this issue, we performed a systematic analysis of fusion protein transcriptional targets. We expressed AML1/ETO, PML/RAR, and PLZF/RAR in U937 hemopoietic precursor cells and measured global gene expression using oligonucleotide chips. We identified 1,555 genes regulated concordantly by at least two fusion proteins that were further validated in patient samples and finally classified according to available functional information. Strikingly, we found that AML fusion proteins induce genes involved in the maintenance of the stem cell phenotype and repress DNA repair genes, mainly of the base excision repair pathway. Functional studies confirmed that ectopic expression of fusion proteins constitutively activates pathways leading to increased stem cell renewal (e.g., the Jagged1/Notch pathway) and provokes accumulation of DNA damage. We propose that expansion of the stem cell compartment and induction of a mutator phenotype are relevant features underlying the leukemic potential of AML-associated fusion proteins.
Myriam Alcalay; Natalia Meani; Vania Gelmetti; Anna Fantozzi; Marta Fagioli; Annette Orleth; Daniela Riganelli; Carla Sebastiani; Enrico Cappelli; Cristina Casciari; Maria Teresa Sciurpi; Angela Rosa Mariano; Simone Paolo Minardi; Lucilla Luzi; Heiko Muller; Pier Paolo Di Fiore; Guido Frosina; Pier Giuseppe Pelicci. Acute myeloid leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair. Journal of Clinical Investigation 2003, 112, 1751 -1761.
AMA StyleMyriam Alcalay, Natalia Meani, Vania Gelmetti, Anna Fantozzi, Marta Fagioli, Annette Orleth, Daniela Riganelli, Carla Sebastiani, Enrico Cappelli, Cristina Casciari, Maria Teresa Sciurpi, Angela Rosa Mariano, Simone Paolo Minardi, Lucilla Luzi, Heiko Muller, Pier Paolo Di Fiore, Guido Frosina, Pier Giuseppe Pelicci. Acute myeloid leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair. Journal of Clinical Investigation. 2003; 112 (11):1751-1761.
Chicago/Turabian StyleMyriam Alcalay; Natalia Meani; Vania Gelmetti; Anna Fantozzi; Marta Fagioli; Annette Orleth; Daniela Riganelli; Carla Sebastiani; Enrico Cappelli; Cristina Casciari; Maria Teresa Sciurpi; Angela Rosa Mariano; Simone Paolo Minardi; Lucilla Luzi; Heiko Muller; Pier Paolo Di Fiore; Guido Frosina; Pier Giuseppe Pelicci. 2003. "Acute myeloid leukemia fusion proteins deregulate genes involved in stem cell maintenance and DNA repair." Journal of Clinical Investigation 112, no. 11: 1751-1761.
Oncogene is one of the world’s leading cancer journals. It is published weekly and covers all aspects of the structure and function of Oncogenes. Oncogene also publishes 8 Reviews issues a year, on a broad range of topics.
Myriam Alcalay; Annette Orleth; Carla Sebastiani; Natalia Meani; Ferdinando Chiaradonna; Cristina Casciari; Maria Teresa Sciurpi; Vania Gelmetti; Daniela Riganelli; Saverio Minucci; Marta Fagioli; Pier Giuseppe Pelicci. Common themes in the pathogenesis of acute myeloid leukemia. Oncogene 2001, 20, 5680 -5694.
AMA StyleMyriam Alcalay, Annette Orleth, Carla Sebastiani, Natalia Meani, Ferdinando Chiaradonna, Cristina Casciari, Maria Teresa Sciurpi, Vania Gelmetti, Daniela Riganelli, Saverio Minucci, Marta Fagioli, Pier Giuseppe Pelicci. Common themes in the pathogenesis of acute myeloid leukemia. Oncogene. 2001; 20 (40):5680-5694.
Chicago/Turabian StyleMyriam Alcalay; Annette Orleth; Carla Sebastiani; Natalia Meani; Ferdinando Chiaradonna; Cristina Casciari; Maria Teresa Sciurpi; Vania Gelmetti; Daniela Riganelli; Saverio Minucci; Marta Fagioli; Pier Giuseppe Pelicci. 2001. "Common themes in the pathogenesis of acute myeloid leukemia." Oncogene 20, no. 40: 5680-5694.
The EGFP‐tk retroviral vector, encoding enhanced green fluorescent protein (EGFP) and the herpes simplex virus thymidine kinase (HSV‐tk) packaged in a Phoenix amphotropic cell line, was used to transduce healthy donor T lymphocytes. Infection yielded a mean of 41·8 ± 9·3% SD (range 31·1–48·4%) EGFP‐positive cells and a mean of 92 ± 2% SD (range 90–94%) after cell sorting. EGFP expression remained stable for 30 d after infection. The entire gene transfer procedure had no significant effect on lymphocyte subsets and slightly reduced clonogenicity. Ganciclovir (gcv) treatment (1 µg/ml × 10 d) killed all EGFP‐positive cells in the transduced and transduced/sorted populations, but had no effect on untransduced controls. Our results show that primary T lymphocytes can be transduced using an EGFP‐tk vector that yields a homogeneous infected population without affecting lymphocyte subsets, function and clonogenicity.
Sabrina Di Florio; Carla Sebastiani; Marta Fagioli; Mauro Di Ianni; Diego Alfonsi; Gigliola Venditti; Pier Giuseppe Pelicci; Antonio Tabilio. Retrovirus-mediated transfer of the herpes simplex virus thymidine kinase and enhanced green fluorescence protein genes in primary T lymphocytes. British Journal of Haematology 2000, 110, 903 -906.
AMA StyleSabrina Di Florio, Carla Sebastiani, Marta Fagioli, Mauro Di Ianni, Diego Alfonsi, Gigliola Venditti, Pier Giuseppe Pelicci, Antonio Tabilio. Retrovirus-mediated transfer of the herpes simplex virus thymidine kinase and enhanced green fluorescence protein genes in primary T lymphocytes. British Journal of Haematology. 2000; 110 (4):903-906.
Chicago/Turabian StyleSabrina Di Florio; Carla Sebastiani; Marta Fagioli; Mauro Di Ianni; Diego Alfonsi; Gigliola Venditti; Pier Giuseppe Pelicci; Antonio Tabilio. 2000. "Retrovirus-mediated transfer of the herpes simplex virus thymidine kinase and enhanced green fluorescence protein genes in primary T lymphocytes." British Journal of Haematology 110, no. 4: 903-906.
The tumour suppressor p53 induces cellular senescence in response to oncogenic signals1. p53 activity is modulated by protein stability and post-translational modification, including phosphorylation and acetylation2. The mechanism of p53 activation by oncogenes remains largely unknown. Here we report that the tumour suppressor PML regulates the p53 response to oncogenic signals. We found that oncogenic Ras upregulates PML expression, and overexpression of PML induces senescence in a p53-dependent manner. p53 is acetylated at lysine 382 upon Ras expression, an event that is essential for its biological function. Ras induces re-localization of p53 and the CBP acetyltransferase within the PML nuclear bodies and induces the formation of a trimeric p53–PML–CBP complex. Lastly, Ras-induced p53 acetylation, p53–CBP complex stabilization and senescence are lost in PML-/-fibroblasts. Our data establish a link between PML and p53 and indicate that integrity of the PML bodies is required for p53 acetylation and senescence upon oncogene expression.
Mark Pearson; Roberta Carbone; Carla Sebastiani; Mario Cioce; Marta Fagioli; Shin’Ichi Saito; Yuichiro Higashimoto; Ettore Appella; Saverio Minucci; Pier Paolo Pandolfi; Pier Giuseppe Pelicci. PML regulates p53 acetylation and premature senescence induced by oncogenic Ras. Nature 2000, 406, 207 -210.
AMA StyleMark Pearson, Roberta Carbone, Carla Sebastiani, Mario Cioce, Marta Fagioli, Shin’Ichi Saito, Yuichiro Higashimoto, Ettore Appella, Saverio Minucci, Pier Paolo Pandolfi, Pier Giuseppe Pelicci. PML regulates p53 acetylation and premature senescence induced by oncogenic Ras. Nature. 2000; 406 (6792):207-210.
Chicago/Turabian StyleMark Pearson; Roberta Carbone; Carla Sebastiani; Mario Cioce; Marta Fagioli; Shin’Ichi Saito; Yuichiro Higashimoto; Ettore Appella; Saverio Minucci; Pier Paolo Pandolfi; Pier Giuseppe Pelicci. 2000. "PML regulates p53 acetylation and premature senescence induced by oncogenic Ras." Nature 406, no. 6792: 207-210.