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Massimo Biagetti
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche-Togo Rosati (IZSUM), Via Salvemini 1, 06126 Perugia, Italy

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Journal article
Published: 01 July 2021 in Viruses
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Maedi-visna virus (MVV) and caprine arthritis encephalitis virus (CAEV), referred to as small ruminant lentiviruses (SRLVs), belong to the genus Lentivirus of the Retroviridae family. SRLVs infect both sheep and goats, causing significant economic losses and animal welfare damage. Recent findings suggest an association between serological status and allelic variants of different genes such as TMEM154, TLR9, MYD88 and CCR5. The aim of this work was to investigate the role of specific polymorphisms of these genes in SRLVs infection in some sheep flocks in Italy. In addition to those already known, novel variants in the TMEM154 (P7H, I74V, I105V) gene were detected in this study. The risk of infection was determined finding an association between the serological status and polymorphisms P7H, E35K, N70I, I74V, I105V of TMEM154, R447Q, A462S and G520R in TLR9 gene, H176H* and K190K* in MYD88 genes, while no statistical association was observed for the 4-bp deletion of the CCR5 gene. Since no vaccines or treatments have been developed, a genetically based approach could be an innovative strategy to prevent and to control SRLVs infection. Our findings are an important starting point in order to define the genetic resistance profile towards SRLVs infection.

ACS Style

Chiara Arcangeli; Daniele Lucarelli; Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Claudia Pellegrini; Andrea Felici; Silva Costarelli; Monica Giammarioli; Francesco Feliziani; Fabrizio Passamonti; Massimo Biagetti. First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection. Viruses 2021, 13, 1290 .

AMA Style

Chiara Arcangeli, Daniele Lucarelli, Martina Torricelli, Carla Sebastiani, Marcella Ciullo, Claudia Pellegrini, Andrea Felici, Silva Costarelli, Monica Giammarioli, Francesco Feliziani, Fabrizio Passamonti, Massimo Biagetti. First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection. Viruses. 2021; 13 (7):1290.

Chicago/Turabian Style

Chiara Arcangeli; Daniele Lucarelli; Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Claudia Pellegrini; Andrea Felici; Silva Costarelli; Monica Giammarioli; Francesco Feliziani; Fabrizio Passamonti; Massimo Biagetti. 2021. "First Survey of SNPs in TMEM154, TLR9, MYD88 and CCR5 Genes in Sheep Reared in Italy and Their Association with Resistance to SRLVs Infection." Viruses 13, no. 7: 1290.

Journal article
Published: 28 January 2021 in Animals
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In goats, as in sheep, genotypes of the prion protein gene (PRNP) can influence animals’ susceptibility to scrapie. Since the polymorphic codons in sheep are well known, a genetic selection plan has been implemented in Europe, in order to reduce the prevalence of susceptible genotypes to scrapie. In Italy, no breeding plan for scrapie resistance in goats has been adopted, yet. Likewise, according to the most recent modification of Regulation EU 999/2001 (Regulation EU 772/2020) of the European Commission (EU), based on all the available experimental and in field data, K222, D146 and S146 polymorphisms could be used as scrapie resistance alleles in genetic management both in scrapie outbreaks and in disease prevention. In order to collect data on the variability of PRNP, the present study aimed to analyze the sequence of the PRNP gene in eight Italian local goat populations/breeds reared in central and southern Italy (Bianca Monticellana, Capestrina, Facciuta della Valnerina, Fulva del Lazio, Garganica, Grigia Ciociara, Grigia Molisana, and Teramana), some of which were investigated for the first time; moreover, two cosmopolitan breeds (Alpine and Saanen) were included. Blood samples were collected from 219 goats. Genomic DNA was extracted from whole blood. DNA was used as template in PCR amplification of the entire PRNP open reading frame (ORF). Purified amplicons have been sequenced and aligned to Capra hircus PRNP. Particularly, the alleles carrying the resistance-related 222 K polymorphism occurred in all populations with a frequency between 2.5% and 12.5%. An additional resistance allele carrying the S146 variant was observed with a frequency of 3.7% only in the Alpine breed. For three of the estimated alleles, we could not establish if the found double polymorphisms in heterozygosis were in phase, due to technical limitations. In this context, in addition to selective culling in scrapie outbreaks according to the European regulation in force, in the future, selection plans could be adopted to deal with scrapie and to control its diffusion, meanwhile paying attention to preserve a high variability of PRNP.

ACS Style

Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Simone Ceccobelli; Barbara Chiappini; Gabriele Vaccari; Antonio Capocefalo; Michela Conte; Samira Giovannini; Emiliano Lasagna; Francesca Sarti; Massimo Biagetti. PRNP Polymorphisms in Eight Local Goat Populations/Breeds from Central and Southern Italy. Animals 2021, 11, 333 .

AMA Style

Martina Torricelli, Carla Sebastiani, Marcella Ciullo, Simone Ceccobelli, Barbara Chiappini, Gabriele Vaccari, Antonio Capocefalo, Michela Conte, Samira Giovannini, Emiliano Lasagna, Francesca Sarti, Massimo Biagetti. PRNP Polymorphisms in Eight Local Goat Populations/Breeds from Central and Southern Italy. Animals. 2021; 11 (2):333.

Chicago/Turabian Style

Martina Torricelli; Carla Sebastiani; Marcella Ciullo; Simone Ceccobelli; Barbara Chiappini; Gabriele Vaccari; Antonio Capocefalo; Michela Conte; Samira Giovannini; Emiliano Lasagna; Francesca Sarti; Massimo Biagetti. 2021. "PRNP Polymorphisms in Eight Local Goat Populations/Breeds from Central and Southern Italy." Animals 11, no. 2: 333.

Communication
Published: 05 February 2020 in Animals
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Bovine milk contains several β-casein variants, with the A1 and A2 variants occurring most frequently. The presence of some variants, such as A1, B, and C, is considered a risk factor for disease in humans who consume milk. These variants are probably involved in intolerance to milk and some human diseases due to the production of a bioactive peptide with opioid activity during digestion, β-casomorphin 7 (BCM-7). In contrast, the A2 variant is not involved in pathogenetic mechanisms; thus, its presence in milk is a desirable feature. The difference between the A1 and A2 variants is a mutation at position 67 of the β-casein gene (CSN2), which causes an amino acid to change from histidine (in the A1, B, and C variants) to proline (in the A2 variant). To select dairy cows on the basis of the presence of the β-casein variant A2, allele frequencies of CSN2 variants were evaluated in Italian dairy cows reared in central Italy. The results of this study may help with the selection of animals with the β-casein gene variant A2 to produce a more digestible milk that only contains the β-casein variant A2. The majority of proteins in cow’s milk are caseins, which occur in four groups (α-s1, α-s2, β, and k) encoded by different genes (CSN1S1, CSN1S2, CSN2, and CSN3, respectively). In this study, we focused on the β-casein allele variants A1 and A2 due to their influence on milk’s technological characteristics and human health. Digestion of the β-casein variant A1 leads to the formation of β-casomorphin 7 (BCM-7), a bioactive peptide that has been suggested to be a possible cause of various human diseases and associated with low milk digestibility. The potential negative role of the β-casein variant A1 in human health has stimulated the planning of cattle breeding programs based on genetic selection to increase the frequency of the A2 variant, which is associated with increased milk digestibility. The aim of this work was to evaluate the frequencies of the different β-casein variants in Italian Holstein Friesian dairy cows from cattle farms located in central Italy to select a population of A2 homozygous animals. β-casein genotypes were identified by evaluating the presence of single nucleotide polymorphisms (SNPs) of the CSN2 gene using PCR and sequencing analysis. The frequency of the desirable β-casein variant A2 in the studied bovine population was 0.61. The frequency of the undesirable A1 variant in the studied bovine population was 0.30. The frequency of the A2 allele was higher than expected for the breed; therefore, genetic selection for the A2 variant in these animals could be achieved in a fairly short time using A2 homozygous bulls.

ACS Style

Carla Sebastiani; Chiara Arcangeli; Marcella Ciullo; Martina Torricelli; Giulia Cinti; Stefano Fisichella; Massimo Biagetti. Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals 2020, 10, 252 .

AMA Style

Carla Sebastiani, Chiara Arcangeli, Marcella Ciullo, Martina Torricelli, Giulia Cinti, Stefano Fisichella, Massimo Biagetti. Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy. Animals. 2020; 10 (2):252.

Chicago/Turabian Style

Carla Sebastiani; Chiara Arcangeli; Marcella Ciullo; Martina Torricelli; Giulia Cinti; Stefano Fisichella; Massimo Biagetti. 2020. "Frequencies Evaluation of β-Casein Gene Polymorphisms in Dairy Cows Reared in Central Italy." Animals 10, no. 2: 252.

Journal article
Published: 01 July 2018 in Talanta
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African swine fever (ASF) virus is a DNA virus responsible for a severe haemorrhagic fever in pigs, which (still in the absence of vaccination strategies) results in high mortality rates. Herein, we present a biosensor-based method for the detection of ASF viral DNA in the blood of pigs. The biosensor exploits a single-strand DNA probe with locked nucleic acid nucleotides (LNA) substitutions as the complementary recognition element for the conserved region of vp72 gene of ASF virus. The biosensor was calibrated using qPCR-quantified ASF viral DNA extracted from the blood of pigs experimentally infected with the virulent Italian isolate 49/08, genotype I. Globally, the proposed biosensor showed good sensitivity and specificity, with the limits of detection (LOD) and quantification (LOQ) being 178 and 245 copies/μL of genomic ASF viral DNA, respectively. The reversible nature of the interaction between the DNA/LNA probe and the target DNA sequence granted multiple rapid analyses, with up to 40 analyses per single surface possible, and a single test requiring approximately 5 min. When applied to non-amplified DNA extracts from the blood of field-infected pigs, the assay discriminated between ASFV-infected and ASFV non-infected animals, and allowed the rapid quantification of ASF viral DNA, with values falling in the range 373–1058 copies/μL of genomic ASFV DNA. In this range, excellent correlation was observed between the results of this biosensor and OIE-approved qPCR. This method represents a promising screening assay for preliminary ASF diagnosis, having the major advantages in the relative rapidity, ease-of-use, the reusability of the sensing surface, and low cost per single test.

ACS Style

Massimo Biagetti; Massimiliano Cuccioloni; Laura Bonfili; Valentina Cecarini; Carla Sebastiani; Ludovica Curcio; Monica Giammarioli; Gian Mario De Mia; Anna Maria Eleuteri; Mauro Angeletti. Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus. Talanta 2018, 184, 35 -41.

AMA Style

Massimo Biagetti, Massimiliano Cuccioloni, Laura Bonfili, Valentina Cecarini, Carla Sebastiani, Ludovica Curcio, Monica Giammarioli, Gian Mario De Mia, Anna Maria Eleuteri, Mauro Angeletti. Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus. Talanta. 2018; 184 ():35-41.

Chicago/Turabian Style

Massimo Biagetti; Massimiliano Cuccioloni; Laura Bonfili; Valentina Cecarini; Carla Sebastiani; Ludovica Curcio; Monica Giammarioli; Gian Mario De Mia; Anna Maria Eleuteri; Mauro Angeletti. 2018. "Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus." Talanta 184, no. : 35-41.

Journal article
Published: 01 May 2018 in Journal of Microbiological Methods
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Abortion in ruminants represents an important economic concern for farmers. Microbial agents, such as Brucella spp., Chlamydia spp., Coxiella burnetii, Leptospira spp., Neospora caninum, Salmonella spp. and Toxoplasma gondii, are among the main infectious causes of abortion and require rapid and reliable diagnosis. This study describes the development of a multi-screening assay using Fast Real-Time PCR (Fast qPCR) that allows, in a single test, the simultaneous identification of the above-mentioned abortive agents. This multi-screening approach is characterized by a mean diagnostic sensitivity and specificity of 100% and 97%, respectively; it has a limit of detection (LOD) ranging from 5 × 103 to 4 × 104 genomic copies/g of tissue and a very good concordance with traditional end-point PCR assays used in routine diagnostic activity. The proposed method represents a rapid approach to the simultaneous detection of the main abortive agents in ruminants that allows to make an accurate diagnosis and to set up appropriate control measures in a short period of time.

ACS Style

Carla Sebastiani; Ludovica Curcio; Marcella Ciullo; Deborah Cruciani; Silvia Crotti; Cristina Pesca; Martina Torricelli; Martina Sebastianelli; Andrea Felici; Massimo Biagetti. A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants. Journal of Microbiological Methods 2018, 148, 12 -17.

AMA Style

Carla Sebastiani, Ludovica Curcio, Marcella Ciullo, Deborah Cruciani, Silvia Crotti, Cristina Pesca, Martina Torricelli, Martina Sebastianelli, Andrea Felici, Massimo Biagetti. A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants. Journal of Microbiological Methods. 2018; 148 ():12-17.

Chicago/Turabian Style

Carla Sebastiani; Ludovica Curcio; Marcella Ciullo; Deborah Cruciani; Silvia Crotti; Cristina Pesca; Martina Torricelli; Martina Sebastianelli; Andrea Felici; Massimo Biagetti. 2018. "A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants." Journal of Microbiological Methods 148, no. : 12-17.

Original article
Published: 24 March 2018 in Journal of Applied Microbiology
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Aims To evaluate the survival of Mycobacterium avium subsp. paratuberculosis (MAP) during anaerobic digestion (AD), we studied two different biogas plants loaded with manure and slurry from paratuberculosis‐infected dairy herds. Methods and Results Plants operated under mesophilic conditions, the first with a single digester and the second with a double digester. MAP detection was performed by sampling each stage of the process, specifically the pre‐fermenter, fermenter, liquid digestate and solid digestate stages, for 11 months. In both plants, MAP was isolated from the pre‐fermenter stage. Only the final products, the solid and liquid digestates, of the one‐stage plant showed viable MAP, while no viable MAP was detected in the digestates of the two‐stage plant. Conclusions MAP showed a significant decrease during subsequent steps of the AD process, particularly in the two‐stage plant. We suggest that the second digester maintained the digestate under anaerobic conditions for a longer period of time, thus reducing MAP survival and MAP load under the culture detection limit. Significance and Impact of the Study Our data are unable to exclude the presence of MAP in the final products of the biogas plants, particularly those products from the single digester; therefore, the use of digestates as fertilizers is a real concern related to the possible environmental contamination with MAP. This article is protected by copyright. All rights reserved.

ACS Style

P. Mazzone; S. Corneli; A. Di Paolo; C. Maresca; A. Felici; M. Biagetti; M. Ciullo; Carla Sebastiani; G. Pezzotti; Simone Leo; M. Ricchi; N. Arrigoni. Survival of Mycobacterium avium subsp. paratuberculosis in the intermediate and final digestion products of biogas plants. Journal of Applied Microbiology 2018, 125, 36 -44.

AMA Style

P. Mazzone, S. Corneli, A. Di Paolo, C. Maresca, A. Felici, M. Biagetti, M. Ciullo, Carla Sebastiani, G. Pezzotti, Simone Leo, M. Ricchi, N. Arrigoni. Survival of Mycobacterium avium subsp. paratuberculosis in the intermediate and final digestion products of biogas plants. Journal of Applied Microbiology. 2018; 125 (1):36-44.

Chicago/Turabian Style

P. Mazzone; S. Corneli; A. Di Paolo; C. Maresca; A. Felici; M. Biagetti; M. Ciullo; Carla Sebastiani; G. Pezzotti; Simone Leo; M. Ricchi; N. Arrigoni. 2018. "Survival of Mycobacterium avium subsp. paratuberculosis in the intermediate and final digestion products of biogas plants." Journal of Applied Microbiology 125, no. 1: 36-44.

Journal article
Published: 01 August 2016 in Journal of Biotechnology
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ACS Style

Massimo Biagetti; Ludovica Curcio; Marcella Ciullo; Carla Sebastiani. Simultaneous identification in fast real time PCR of the main abortive agents of ruminants. Journal of Biotechnology 2016, 231, S73 .

AMA Style

Massimo Biagetti, Ludovica Curcio, Marcella Ciullo, Carla Sebastiani. Simultaneous identification in fast real time PCR of the main abortive agents of ruminants. Journal of Biotechnology. 2016; 231 ():S73.

Chicago/Turabian Style

Massimo Biagetti; Ludovica Curcio; Marcella Ciullo; Carla Sebastiani. 2016. "Simultaneous identification in fast real time PCR of the main abortive agents of ruminants." Journal of Biotechnology 231, no. : S73.

Review
Published: 01 January 2016 in Animal
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Scrapie is a naturally occurring transmissible spongiform encephalopathy in sheep and goat. It has been known for ~250 years and is characterised by the accumulation of an abnormal isoform of a host-encoded prion protein that leads to progressive neurodegeneration and death. Scrapie is recognised in two forms, classical and atypical scrapie. The susceptibility to both types of scrapie is influenced by polymorphisms of the prion protein gene (PRNP). Sheep susceptibility or resistance to classical scrapie is strongly regulated by the polymorphisms at codons 136, 154 and 171 of the PRNP. The genetic role in atypical scrapie in sheep has been defined by polymorphisms at codons 141, 154 and 171, which are associated with different degrees of risk in the occurrence of the ovine disease. Progress has been achieved in the prevention of scrapie in sheep due to efficient genetic breeding programmes based on eradication and control of the disease. In Europe, the success of these programmes has been verified by applying eradication and genetic selection plans. In general terms, the ovine selection plans aim to eliminate and reduce the susceptible allele and to enrich the resistant allele ARR. During outbreaks all susceptible animals are slaughtered, only ARR/ARR resistant rams and sheep and semi-resistant females are preserved. In the occurrence of scrapie positive goats a complete cull of the flock (stamping out) is performed with great economic loss and severe risk of extinction for the endangered breeds. The ability to select scrapie-resistant animals allows to define new breeding strategies aimed to boost genetic progress while reducing costs during scrapie outbreaks. Allelic variants of PRNP can be protective for caprine scrapie, and the knowledge of their distribution in goats has become very important. Over the past few years, the integration of genetic information on goat populations could be used to make selection decisions, commonly referred to as genetic selection. The objective of this review was to summarise the main findings of polymorphisms of the caprine prion protein (PrP) gene and to discuss the possible application of goat breeding schemes integrating genetic selection, with their relative advantages and limitations.

ACS Style

L. Curcio; Carla Sebastiani; P. Di Lorenzo; E. Lasagna; Massimo Biagetti. Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease. Animal 2016, 10, 1585 -1593.

AMA Style

L. Curcio, Carla Sebastiani, P. Di Lorenzo, E. Lasagna, Massimo Biagetti. Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease. Animal. 2016; 10 (10):1585-1593.

Chicago/Turabian Style

L. Curcio; Carla Sebastiani; P. Di Lorenzo; E. Lasagna; Massimo Biagetti. 2016. "Review: A review on classical and atypical scrapie in caprine: Prion protein gene polymorphisms and their role in the disease." Animal 10, no. 10: 1585-1593.

Journal article
Published: 01 November 2015 in Livestock Science
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Ludovica Curcio; Carla Sebastiani; Simone Ceccobelli; Gabriele Vaccari; Giovanni Pezzotti; Emiliano Lasagna; Massimo Biagetti. PRNP polymorphisms in four Italian sheep breeds. Livestock Science 2015, 181, 38 -42.

AMA Style

Ludovica Curcio, Carla Sebastiani, Simone Ceccobelli, Gabriele Vaccari, Giovanni Pezzotti, Emiliano Lasagna, Massimo Biagetti. PRNP polymorphisms in four Italian sheep breeds. Livestock Science. 2015; 181 ():38-42.

Chicago/Turabian Style

Ludovica Curcio; Carla Sebastiani; Simone Ceccobelli; Gabriele Vaccari; Giovanni Pezzotti; Emiliano Lasagna; Massimo Biagetti. 2015. "PRNP polymorphisms in four Italian sheep breeds." Livestock Science 181, no. : 38-42.

Journal article
Published: 01 January 2015 in Italian Journal of Animal Science
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ACS Style

Ludovica Curcio; Emiliano Lasagna; Francesca Maria Sarti; Carla Sebastiani; Giovanni Pezzotti; Massimo Biagetti. Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed. Italian Journal of Animal Science 2015, 14, 4251 .

AMA Style

Ludovica Curcio, Emiliano Lasagna, Francesca Maria Sarti, Carla Sebastiani, Giovanni Pezzotti, Massimo Biagetti. Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed. Italian Journal of Animal Science. 2015; 14 (4):4251.

Chicago/Turabian Style

Ludovica Curcio; Emiliano Lasagna; Francesca Maria Sarti; Carla Sebastiani; Giovanni Pezzotti; Massimo Biagetti. 2015. "Biodiversity and Genetic Polymorphisms Against Scrapie inSopravissanaSheep Breed." Italian Journal of Animal Science 14, no. 4: 4251.

Journal article
Published: 08 February 2012 in Journal of Clinical Microbiology
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Bovine tuberculosis (bTB) is an emerging disease among wild animals in many parts of the world. Wildlife reservoir hosts may thus represent a potential source of infection for livestock and humans. We investigated the role played by the Sicilian black pig, an autochthonous free- or semi-free-ranging domestic pig breed, as a potential source of bTB infection in an area where bTB prevalence in cattle is high. We initially performed a preliminary field study to assess the occurrence of bTB in such animals. We sampled 119 pigs at abattoir and found 6.7% and 3.4% of them to be affected by gross tuberculous-like lesions (TBL) and Mycobacterium bovis culture positive, respectively. We then proceeded to investigate the dissemination and characteristics of lesions in a second field study performed on 100 animals sampled from infected herds. Here, tissues collected at the abattoir were examined macroscopically, microscopically, and by culture tests. Most pigs with TBL showed generalized lesions in both gross and histological examinations (53% and 65.5%, respectively). Head lymph nodes were the most frequently affected in both localized and generalized TB cases observed macroscopically and microscopically. M. bovis was the most frequently isolated etiologic agent. The molecular characterization of isolates from both field studies by spoligotyping and analysis of 12 mycobacterial interspersed repetitive-unit–variable number tandem repeat (MIRU-VNTR) loci, followed by their comparison to isolates of cattle origin, suggested a potential transmission of mycobacteria from domestic animals to black pigs and vice versa. Our findings, along with ethological, ecological, and management considerations, suggest that the black pig might act as a bTB reservoir in the ecosystem under study. However, additional studies will be necessary to establish the true epidemiological significance of the Sicilian black pig.

ACS Style

Vincenzo Di Marco; Piera Mazzone; Maria Teresa Capucchio; Maria Beatrice Boniotti; Vincenzo Aronica; Miriam Russo; Michele Fiasconaro; Noemi Cifani; Sara Corneli; Elena Biasibetti; Massimo Biagetti; Maria Lodovica Pacciarini; Monica Cagiola; Paolo Pasquali; Cinzia Marianelli. Epidemiological Significance of the Domestic Black Pig (Sus scrofa) in Maintenance of Bovine Tuberculosis in Sicily. Journal of Clinical Microbiology 2012, 50, 1209 -1218.

AMA Style

Vincenzo Di Marco, Piera Mazzone, Maria Teresa Capucchio, Maria Beatrice Boniotti, Vincenzo Aronica, Miriam Russo, Michele Fiasconaro, Noemi Cifani, Sara Corneli, Elena Biasibetti, Massimo Biagetti, Maria Lodovica Pacciarini, Monica Cagiola, Paolo Pasquali, Cinzia Marianelli. Epidemiological Significance of the Domestic Black Pig (Sus scrofa) in Maintenance of Bovine Tuberculosis in Sicily. Journal of Clinical Microbiology. 2012; 50 (4):1209-1218.

Chicago/Turabian Style

Vincenzo Di Marco; Piera Mazzone; Maria Teresa Capucchio; Maria Beatrice Boniotti; Vincenzo Aronica; Miriam Russo; Michele Fiasconaro; Noemi Cifani; Sara Corneli; Elena Biasibetti; Massimo Biagetti; Maria Lodovica Pacciarini; Monica Cagiola; Paolo Pasquali; Cinzia Marianelli. 2012. "Epidemiological Significance of the Domestic Black Pig (Sus scrofa) in Maintenance of Bovine Tuberculosis in Sicily." Journal of Clinical Microbiology 50, no. 4: 1209-1218.

Journal article
Published: 01 June 2007 in Parassitologia
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ACS Style

Fabio Macchioni; Franco Cecchi; Roberta Ciampolini; Massimo Biagetti; Elena Ciani; G Filippini; P Papa; Carla Sebastiani; D Cianci. The genetic resistance to gastro-intestinal strongylids in Appenninica sheep: preliminary results. Parassitologia 2007, 49, 1 .

AMA Style

Fabio Macchioni, Franco Cecchi, Roberta Ciampolini, Massimo Biagetti, Elena Ciani, G Filippini, P Papa, Carla Sebastiani, D Cianci. The genetic resistance to gastro-intestinal strongylids in Appenninica sheep: preliminary results. Parassitologia. 2007; 49 (1):1.

Chicago/Turabian Style

Fabio Macchioni; Franco Cecchi; Roberta Ciampolini; Massimo Biagetti; Elena Ciani; G Filippini; P Papa; Carla Sebastiani; D Cianci. 2007. "The genetic resistance to gastro-intestinal strongylids in Appenninica sheep: preliminary results." Parassitologia 49, no. 1: 1.

Journal article
Published: 01 January 2007 in Biological Chemistry
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Aflatoxins (AF) are contaminants of improperly stored foods; they are potent genotoxic and carcinogenic compounds, exerting their effects through damage to DNA. They can also induce mutations that increase oxidative damage. The goal of this study was to evaluate the possibility that a third mechanism could be involved in the carcinogenic action of aflatoxins, namely, direct binding to key enzymes involved in the regulatory pathways of the cell cycle, thereby modulating enzyme functionality. The 20S constitutive and immunoproteasome peptidase and proteolytic activities were assayed in the presence of aflatoxins B1, G1 and M1. All three toxins activated multiple peptidase activities of the proteasome. Aflatoxin (AF) M1 was the most potent activator of proteasome activity, while the constitutive 20S proteasome was specifically stimulated by AFG1. Furthermore, the effects of AFB1 on cultured hepatoma cells were investigated and the various proteasomal activities determined with cell lysates were differently affected. Taking into account the key role of the proteasome in cellular defense against oxidative stress, the carbonyl group content and the activities of antioxidant enzymes in cell lysates were analyzed. The proapoptotic effect of AFB1 was also investigated by measuring caspase-3 activity and cellular levels of p27 and IkappaBalpha.

ACS Style

Manila Amici; Valentina Cecarini; Assuntina Pettinari; Laura Bonfili; Mauro Angeletti; Simone Barocci; Massimo Biagetti; Evandro Fioretti; Anna Maria Eleuteri. Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis. Biological Chemistry 2007, 388, 1 .

AMA Style

Manila Amici, Valentina Cecarini, Assuntina Pettinari, Laura Bonfili, Mauro Angeletti, Simone Barocci, Massimo Biagetti, Evandro Fioretti, Anna Maria Eleuteri. Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis. Biological Chemistry. 2007; 388 (1):1.

Chicago/Turabian Style

Manila Amici; Valentina Cecarini; Assuntina Pettinari; Laura Bonfili; Mauro Angeletti; Simone Barocci; Massimo Biagetti; Evandro Fioretti; Anna Maria Eleuteri. 2007. "Binding of aflatoxins to the 20S proteasome: effects on enzyme functionality and implications for oxidative stress and apoptosis." Biological Chemistry 388, no. 1: 1.

Journal article
Published: 01 August 2006 in Veterinary Research Communications
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ACS Style

G. Filippini; V. Cetica; Roberta Ciampolini; M. Biagetti; Francesca Cecchi; E. Mazzanti; Elena Ciani; Carla Sebastiani; G. Venditti. Beef Traceability Using Molecular Methodologies. Veterinary Research Communications 2006, 30, 375 -377.

AMA Style

G. Filippini, V. Cetica, Roberta Ciampolini, M. Biagetti, Francesca Cecchi, E. Mazzanti, Elena Ciani, Carla Sebastiani, G. Venditti. Beef Traceability Using Molecular Methodologies. Veterinary Research Communications. 2006; 30 (1):375-377.

Chicago/Turabian Style

G. Filippini; V. Cetica; Roberta Ciampolini; M. Biagetti; Francesca Cecchi; E. Mazzanti; Elena Ciani; Carla Sebastiani; G. Venditti. 2006. "Beef Traceability Using Molecular Methodologies." Veterinary Research Communications 30, no. 1: 375-377.

Molecular genetics
Published: 01 January 2006 in Journal of Animal Science
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Assignment tests based on multilocus genotypes are becoming increasingly important to certify quality and origin of livestock products and assure food safety and authenticity. The purpose of this study was to determine the potential of microsatellites (STR) for determining the breed origin of beef products among cattle breeds present in the market. We typed 19 STR in 269 animals from 4 cattle breeds. Based on Wright's F-statistics, 4 loci were discarded, and the remaining 15 loci (FIT = 0.101, FST = 0.089, and FIS = 0.013) were used to compute the likelihood that each multilocus genotype of the total sample was drawn from its true breed instead of another breed. To avoid occurrence of zero likelihood when one or more alleles were missing from a tested breed, sample allele frequencies were estimated assuming uniform prior distributions. Log-likelihood ratio [log(LR)] distributions of the individual assignments were determined for all possible breed contrasts, and their means and SD were used to infer the true-positive and false-positive rates at several values of the log(LR). The posterior probability that the animals of a presumed breed were actually drawn from that breed instead of any another breed was then calculated. Given an observed value of log(LR) > 0 and assuming equal priors, these probabilities were >99.5% in 10 of 12 possible breed contrasts. For the 2 most closely related breeds (FST = 0.041), this probability was 96.3%, and the probability of excluding the origin of an animal from an alleged breed when it was actually derived from another breed was similar.

ACS Style

Roberta Ciampolini; V. Cetica; Elena Ciani; E. Mazzanti; X. Fosella; F. Marroni; M. Biagetti; Carla Sebastiani; P. Papa; G. Filippini; D. Cianci; S. Presciuttini. Statistical analysis of individual assignment tests among four cattle breeds using fifteen STR loci1. Journal of Animal Science 2006, 84, 11 -19.

AMA Style

Roberta Ciampolini, V. Cetica, Elena Ciani, E. Mazzanti, X. Fosella, F. Marroni, M. Biagetti, Carla Sebastiani, P. Papa, G. Filippini, D. Cianci, S. Presciuttini. Statistical analysis of individual assignment tests among four cattle breeds using fifteen STR loci1. Journal of Animal Science. 2006; 84 (1):11-19.

Chicago/Turabian Style

Roberta Ciampolini; V. Cetica; Elena Ciani; E. Mazzanti; X. Fosella; F. Marroni; M. Biagetti; Carla Sebastiani; P. Papa; G. Filippini; D. Cianci; S. Presciuttini. 2006. "Statistical analysis of individual assignment tests among four cattle breeds using fifteen STR loci1." Journal of Animal Science 84, no. 1: 11-19.

Journal article
Published: 17 December 2004 in Proteins: Structure, Function, and Bioinformatics
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Transmissible spongiform encephalopathies are a class of sporadic, genetic and transmissible neurodegenerative diseases that affect both humans and animals. Propagation of these diseases is thought to be due to the misfolding of a neuronal glyco-protein, PrP(c), into a pathological insoluble conformer, PrP(Sc). In earlier works, some serum components were identified as exclusive PrP(Sc)-interacting proteins (Fisher et al., Nature 2000;408:479), and thus those macromolecules were thought to represent a potential diagnostic endogenous factor discriminating between normal and pathological prion proteins. In contrast, in agreement with a recent work (Kornblatt et al., Biochem Biophys Res Commun 2003;305:518), in this paper we present a detailed thermodynamic and kinetic characterization of the interaction between recombinant bovine PrP(c 25-242) and the human serum component plasminogen, measured using a resonant mirror technique: our results reveal a high-affinity interaction between the two binding partners. For comparison, the complex obtained from the purified full-length PrP(c) and human plasminogen was also studied: both prion proteins (the recombinant bovine PrP(c 25-242) and the purified full-length PrP(c)) are able to bind human plasminogen. Both kinetic and thermodynamic parameters are affected by the modulation exerted by the H(+) ions in solution. Moreover, the analysis of binding, according to canonical linkage relationships, suggests the involvement of a His residue, consistent with the interaction between other serine (pro)enzymes and their ligands.

ACS Style

Massimiliano Cuccioloni; Manila Amici; Anna Maria Eleuteri; Massimo Biagetti; Simone Barocci; Mauro Angeletti. Binding of recombinant PrPc to human plasminogen: Kinetic and thermodynamic study using a resonant mirror biosensor. Proteins: Structure, Function, and Bioinformatics 2004, 58, 728 -734.

AMA Style

Massimiliano Cuccioloni, Manila Amici, Anna Maria Eleuteri, Massimo Biagetti, Simone Barocci, Mauro Angeletti. Binding of recombinant PrPc to human plasminogen: Kinetic and thermodynamic study using a resonant mirror biosensor. Proteins: Structure, Function, and Bioinformatics. 2004; 58 (3):728-734.

Chicago/Turabian Style

Massimiliano Cuccioloni; Manila Amici; Anna Maria Eleuteri; Massimo Biagetti; Simone Barocci; Mauro Angeletti. 2004. "Binding of recombinant PrPc to human plasminogen: Kinetic and thermodynamic study using a resonant mirror biosensor." Proteins: Structure, Function, and Bioinformatics 58, no. 3: 728-734.

Comparative study
Published: 20 September 2001 in Veterinary Microbiology
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To gain an insight into the molecular epidemiology of classical swine fever (CSF) in Italy, virus isolates originating from outbreaks that occurred between 1985 and 2000 in wild boar or in domestic pigs in mainland Italy and in Sardinia were analysed by genetic typing. For this, a fragment (190 nucleotides) of the E2 glycoprotein gene was sequenced and phylogenetic analyses were performed, including older Italian isolates and isolates from recent outbreaks in Europe for comparison. The results show that in mainland Italy, several independent epidemiological events occurred in the last decade. In the north of the country, viruses of genotype 2.2 have persisted in wild boar, causing sporadic outbreaks in domestic pigs. In contrast, viruses of subgroups 2.1 and 2.3 appeared only intermittently in different regions of the mainland. In 1997, classical swine fever virus (CSFV) isolates belonging to the subgroup 2.1 and genetically and epidemiologically related to the Dutch isolate in Venhorst, affected domestic pigs exclusively. The isolates of subgroup 2.3, derived from wild boar as well as from domestic pigs were closely related to isolates from Germany and Poland. In Sardinia, CSF is an endemic in wild boar and affects domestic pigs also. Genetic typing showed that viruses of subgroups 1.1 and 2.3 have been present, the last ones being unrelated to the mainland viruses of the same subgroup. Due to the large quantities of pig and wild boar meat imported in some parts of Italy, it cannot be established if these viruses were always present in either the mainland or Sardinia, or if they represent recent introductions.

ACS Style

Massimo Biagetti; Irene Greiser-Wilke; Domenico Rutili. Molecular epidemiology of classical swine fever in Italy. Veterinary Microbiology 2001, 83, 205 -215.

AMA Style

Massimo Biagetti, Irene Greiser-Wilke, Domenico Rutili. Molecular epidemiology of classical swine fever in Italy. Veterinary Microbiology. 2001; 83 (3):205-215.

Chicago/Turabian Style

Massimo Biagetti; Irene Greiser-Wilke; Domenico Rutili. 2001. "Molecular epidemiology of classical swine fever in Italy." Veterinary Microbiology 83, no. 3: 205-215.

Journal article
Published: 24 April 2000 in Veterinary Microbiology
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Six laboratories participated in an exercise to compare the sensitivity and specificity of RT-PCR tests for the detection of classical swine fever virus (CSFV). Two sets of coded samples were prepared by serial dilution of positive samples and then distributed to each of the laboratories. One set comprised 34 samples of random primed cDNA. These had been synthesised from viral RNA representative of seven different genetic subtypes of CSFV. The other set comprised 40 clinical samples containing tonsil, spleen, whole blood or serum from a pig that had been experimentally infected with CSFV. Each laboratory tested the samples using one or more PCR/RT-PCR tests that they were accustomed to using. The methods and results of the laboratories were compared with one another. The RT-PCR results obtained from testing the clinical samples were also compared with those obtained by virus isolation and antigen ELISA. The cDNA from three CSFV isolates was detected poorly or not at all by some of the PCR tests. For clinical samples, the order of sensitivity was RT-nested PCR>RT-PCR>virus isolation>ELISA. Both RT-PCR and RT-nested PCR appeared to give some false positive results. Several of the PCR tests appear suitable in terms of specificity and sensitivity. Further trials are necessary to compare results when the same test is performed by different laboratories, and to show that improved control procedures can eliminate problems due to false positive reactions. A limited comparison of extraction and reverse transcription procedures showed similar results in each of three participating laboratories, even though the methods were not standardised.

ACS Style

D.J Paton; A McGoldrick; S Belak; C Mittelholzer; F Koenen; H Vanderhallen; Massimo Biagetti; G.-M De Mia; Tomasz Stadejek; M.A Hofmann; B Thuer. Classical swine fever virus: a ring test to evaluate RT-PCR detection methods. Veterinary Microbiology 2000, 73, 159 -174.

AMA Style

D.J Paton, A McGoldrick, S Belak, C Mittelholzer, F Koenen, H Vanderhallen, Massimo Biagetti, G.-M De Mia, Tomasz Stadejek, M.A Hofmann, B Thuer. Classical swine fever virus: a ring test to evaluate RT-PCR detection methods. Veterinary Microbiology. 2000; 73 (2-3):159-174.

Chicago/Turabian Style

D.J Paton; A McGoldrick; S Belak; C Mittelholzer; F Koenen; H Vanderhallen; Massimo Biagetti; G.-M De Mia; Tomasz Stadejek; M.A Hofmann; B Thuer. 2000. "Classical swine fever virus: a ring test to evaluate RT-PCR detection methods." Veterinary Microbiology 73, no. 2-3: 159-174.

Journal article
Published: 01 April 1994 in Cell Proliferation
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The expression of two oncogenes, c-myc and c-fos, was studied in an ascitic tumour (ATPC+) at different times after implantation. The specific mRNA synthesis was analysed by Northern blot analysis. The presence of the oncogene proteins was shown by immunofluorescence using flow cytometry and referred to the distribution of the cells in the different cell phases. The results show that both oncogenes are expressed by ATPC+ tumour cells. c-myc is expressed 5, 8 and 12 days after implantation, although with a different intensity, and the protein is mainly present in S or S+G2 phase cells. The c-fos oncogene is expressed only 12 days after tumour implantation and the cells labelled with the specific antibody are mainly in G1 phase. We conclude that c-myc is principally correlated with proliferative activity, whereas c-fos is expressed by non-cycling cells.

ACS Style

M. Biagetti; Maria Agnese Della Fazia; Giuseppe Servillo; M. P. Viola‐Magni. Changes in oncogene expression in ascite tumour cells during ageing. Cell Proliferation 1994, 27, 191 -200.

AMA Style

M. Biagetti, Maria Agnese Della Fazia, Giuseppe Servillo, M. P. Viola‐Magni. Changes in oncogene expression in ascite tumour cells during ageing. Cell Proliferation. 1994; 27 (4):191-200.

Chicago/Turabian Style

M. Biagetti; Maria Agnese Della Fazia; Giuseppe Servillo; M. P. Viola‐Magni. 1994. "Changes in oncogene expression in ascite tumour cells during ageing." Cell Proliferation 27, no. 4: 191-200.

Journal article
Published: 01 November 1991 in Cell Proliferation
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The growth of ATPC+, an ascites tumour derived from a spontaneous mammary carcinoma in BALB/c+ mice, was studied at different ages. It was observed that the number of cells increases rapidly during the first 5 days after implantation. Thereafter, the cell number increases more slowly, reaching a plateau after 8 days. This slowing-down is not due to a reduction in the growth fraction but to a lengthening of the cell cycle. Between 5 and 8 days the duration of all phases increases, including the S phase, which increases from 5.2 h in 5-day tumours to 8.2 h in 8-day tumours. In 12-day tumours both the cell cycle and S phase are only slightly longer than in 8-day tumours whereas the growth fraction is reduced. The slowing-down of cell growth can be attributed to growth fraction reduction rather than cell loss, which is maximal in the 5-day tumour. At this age the time course of the percent labelled cells and of the number of grains/nucleus suggests reutilization of [3H]-thymidine. Incorporation of [3H]-thymidine/cell decreases sharply in 12-day tumours due to a reduced availability of thymidine, which is degraded to thymine in the in vivo ascitic fluid faster than in 8-day tumours. This indicates an age-related change in the ascitic fluid composition.

ACS Style

M. P. Viola‐Magni; M. Biagetti. Age-related changes in the cell proliferation of ATPC+mouse ascites tumour. Cell Proliferation 1991, 24, 557 -567.

AMA Style

M. P. Viola‐Magni, M. Biagetti. Age-related changes in the cell proliferation of ATPC+mouse ascites tumour. Cell Proliferation. 1991; 24 (6):557-567.

Chicago/Turabian Style

M. P. Viola‐Magni; M. Biagetti. 1991. "Age-related changes in the cell proliferation of ATPC+mouse ascites tumour." Cell Proliferation 24, no. 6: 557-567.