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Begona Miras-Moreno
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, I-29122, Piacenza, Italy

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Journal article
Published: 02 August 2021 in Plant Science
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Agrochemicals are commonly used in agriculture to protect crops and ensure yields. Several of them are mobile within the plant and, being perceived as xenobiotics regardless of their protective/curative roles, they induce a reprogramming of secondary metabolism linked to the detoxification processes even in the absence of phenotype symptoms. Moreover, it is well documented that plants are able to shape the microbial population at the rhizosphere and to significantly affect the processes occurring therein thanks to the root exudation of different metabolites. Here we show that plant metabolic response to foliarly-applied pesticides is much broader than what previously thought and includes diverse and compound-specific hidden processes. Among others, stress-related metabolism and phytohormones profile underwent a considerable reorganization. Moreover, a distinctive microbial rearrangement of the rhizosphere was recorded following foliar application of pesticides. Such effects have unavoidably energetic and metabolic costs for the plant paving the way to both positive and negative aspects. The understanding of these effects is crucial for an increasingly sustainable use of pesticides in agriculture.

ACS Style

Stefano Cesco; Luigi Lucini; Begona Miras-Moreno; Luigimaria Borruso; Tanja Mimmo; Youry Pii; Edoardo Puglisi; Giulia Spini; Eren Taskin; Raphael Tiziani; Maria Simona Zangrillo; Marco Trevisan. The hidden effects of agrochemicals on plant metabolism and root-associated microorganisms. Plant Science 2021, 311, 111012 .

AMA Style

Stefano Cesco, Luigi Lucini, Begona Miras-Moreno, Luigimaria Borruso, Tanja Mimmo, Youry Pii, Edoardo Puglisi, Giulia Spini, Eren Taskin, Raphael Tiziani, Maria Simona Zangrillo, Marco Trevisan. The hidden effects of agrochemicals on plant metabolism and root-associated microorganisms. Plant Science. 2021; 311 ():111012.

Chicago/Turabian Style

Stefano Cesco; Luigi Lucini; Begona Miras-Moreno; Luigimaria Borruso; Tanja Mimmo; Youry Pii; Edoardo Puglisi; Giulia Spini; Eren Taskin; Raphael Tiziani; Maria Simona Zangrillo; Marco Trevisan. 2021. "The hidden effects of agrochemicals on plant metabolism and root-associated microorganisms." Plant Science 311, no. : 111012.

Journal article
Published: 30 July 2021 in Agronomy
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Protein hydrolysates (PHs) are employed in agriculture to increase the sustainability of farming systems, with positive results on crop productivity and response against environmental stressors. Nevertheless, the molecular mechanism(s) triggered by their specific activity is not clearly understood. In this work, five PHs obtained by enzymatic hydrolysis of different vegetal protein sources were tested for their root-promoting activity on tomato cuttings. All the treatments improved both root length and number when compared to negative controls. Distinctive metabolomic signatures were highlighted in response to treatments, indicating the triggering of different molecular processes in leaf tissues of tomato cuttings. PHs differentially modulated the biosynthesis of plant stress-protectants, such as alkaloids and phenylpropanoids. Moreover, metabolites involved in phytohormone biosynthesis were significantly impacted. In this context, a clear modulation of several compounds related to auxin homeostasis was observed. In addition, the differential modulation of SlIAA2 and SlIAA9 genes, which are involved in the IAA signalling pathway, might further suggest the auxin-like activity elicited by the PHs tested. Here we provide evidence that PHs can impact plant molecular level, positively affecting root development, most likely by affecting the signalling cascades activated in leaf tissues. The biostimulant activity was sustained by PH-specific response at the molecular level, likely ascribable to their heterogeneous botanical origins. In fact, our findings did not point out a clear universal response to PHs, and specific effects are to be investigated.

ACS Style

Valentina Buffagni; Angela Ceccarelli; Youry Pii; Begoña Miras-Moreno; Youssef Rouphael; Mariateresa Cardarelli; Giuseppe Colla; Luigi Lucini. The Modulation of Auxin-Responsive Genes, Phytohormone Profile, and Metabolomic Signature in Leaves of Tomato Cuttings Is Specifically Modulated by Different Protein Hydrolysates. Agronomy 2021, 11, 1524 .

AMA Style

Valentina Buffagni, Angela Ceccarelli, Youry Pii, Begoña Miras-Moreno, Youssef Rouphael, Mariateresa Cardarelli, Giuseppe Colla, Luigi Lucini. The Modulation of Auxin-Responsive Genes, Phytohormone Profile, and Metabolomic Signature in Leaves of Tomato Cuttings Is Specifically Modulated by Different Protein Hydrolysates. Agronomy. 2021; 11 (8):1524.

Chicago/Turabian Style

Valentina Buffagni; Angela Ceccarelli; Youry Pii; Begoña Miras-Moreno; Youssef Rouphael; Mariateresa Cardarelli; Giuseppe Colla; Luigi Lucini. 2021. "The Modulation of Auxin-Responsive Genes, Phytohormone Profile, and Metabolomic Signature in Leaves of Tomato Cuttings Is Specifically Modulated by Different Protein Hydrolysates." Agronomy 11, no. 8: 1524.

Journal article
Published: 16 July 2021 in Plants
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The quest for sustainable strategies aimed at increasing the bioactive properties of plant-based foods has grown quickly. In this work, we investigated the impact of exogenously applied phenolics, i.e., chlorogenic acid (CGA), hesperidin (HES), and their combinations (HES + CGA), on Lactuca sativa L. grown under normal- and mild-salinity conditions. To this aim, the phenolic profile, antioxidant properties, and enzyme inhibitory activity were determined. The untargeted metabolomics profiling revealed that lettuce treated with CGA under non-stressed conditions exhibited the highest total phenolic content (35.98 mg Eq./g). Lettuce samples grown under salt stress showed lower phenolic contents, except for lettuce treated with HES or HES + CGA, when comparing the same treatment between the two conditions. Furthermore, the antioxidant capacity was investigated through DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,20-azinobis-(3-ethylbenzothiazoline-6-sulfonate)), and FRAP (ferric reducing antioxidant power) assays, coupled with metal-chelating activity and phosphomolybdenum capacity. An exciting increase in radical scavenging capacity was observed in lettuce treated with exogenous phenolics, in both stress and non-stress conditions. The inhibitory activity of the samples was evaluated against target health-related enzymes, namely cholinesterases (acetylcholinesterase; AChE; butyryl cholinesterase; BChE), tyrosinase, α-amylase, and α-glucosidase. Lettuce treated with HES + CGA under non-stress conditions exhibited the strongest inhibition against AChE and BChE, while the same treatment under salinity conditions resulted in the highest inhibition capacity against α-amylase. Additionally, CGA under non-stress conditions exhibited the best inhibitory effect against tyrosinase. All the functional traits investigated were significantly modulated by exogenous phenolics, salinity, and their combination. In more detail, flavonoids, lignans, and stilbenes were the most affected phenolics, whereas glycosidase enzymes and tyrosinase activity were the most affected among enzyme assays. In conclusion, the exogenous application of phenolics to lettuce represents an effective and green strategy to effectively modulate the phenolic profile, antioxidant activity, and enzyme inhibitory effects in lettuce, deserving future application to produce functional plant-based foods in a sustainable way.

ACS Style

Leilei Zhang; Erika Martinelli; Biancamaria Senizza; Begoña Miras-Moreno; Evren Yildiztugay; Busra Arikan; Fevzi Elbasan; Gunes Ak; Melike Balci; Gokhan Zengin; Youssef Rouphael; Luigi Lucini. The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce. Plants 2021, 10, 1457 .

AMA Style

Leilei Zhang, Erika Martinelli, Biancamaria Senizza, Begoña Miras-Moreno, Evren Yildiztugay, Busra Arikan, Fevzi Elbasan, Gunes Ak, Melike Balci, Gokhan Zengin, Youssef Rouphael, Luigi Lucini. The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce. Plants. 2021; 10 (7):1457.

Chicago/Turabian Style

Leilei Zhang; Erika Martinelli; Biancamaria Senizza; Begoña Miras-Moreno; Evren Yildiztugay; Busra Arikan; Fevzi Elbasan; Gunes Ak; Melike Balci; Gokhan Zengin; Youssef Rouphael; Luigi Lucini. 2021. "The Combination of Mild Salinity Conditions and Exogenously Applied Phenolics Modulates Functional Traits in Lettuce." Plants 10, no. 7: 1457.

Journal article
Published: 07 July 2021 in International Journal of Molecular Sciences
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Coumarin is a phytotoxic natural compound able to affect plant growth and development. Previous studies have demonstrated that this molecule at low concentrations (100 µM) can reduce primary root growth and stimulate lateral root formation, suggesting an auxin-like activity. In the present study, we evaluated coumarin’s effects (used at lateral root-stimulating concentrations) on the root apical meristem and polar auxin transport to identify its potential mode of action through a confocal microscopy approach. To achieve this goal, we used several Arabidopsis thaliana GFP transgenic lines (for polar auxin transport evaluation), immunolabeling techniques (for imaging cortical microtubules), and GC-MS analysis (for auxin quantification). The results highlighted that coumarin induced cyclin B accumulation, which altered the microtubule cortical array organization and, consequently, the root apical meristem architecture. Such alterations reduced the basipetal transport of auxin to the apical root apical meristem, inducing its accumulation in the maturation zone and stimulating lateral root formation.

ACS Style

Leonardo Bruno; Emanuela Talarico; Luz Cabeiras-Freijanes; Maria Madeo; Antonella Muto; Marco Minervino; Luigi Lucini; Begoña Miras-Moreno; Adriano Sofo; Fabrizio Araniti. Coumarin Interferes with Polar Auxin Transport Altering Microtubule Cortical Array Organization in Arabidopsis thaliana (L.) Heynh. Root Apical Meristem. International Journal of Molecular Sciences 2021, 22, 7305 .

AMA Style

Leonardo Bruno, Emanuela Talarico, Luz Cabeiras-Freijanes, Maria Madeo, Antonella Muto, Marco Minervino, Luigi Lucini, Begoña Miras-Moreno, Adriano Sofo, Fabrizio Araniti. Coumarin Interferes with Polar Auxin Transport Altering Microtubule Cortical Array Organization in Arabidopsis thaliana (L.) Heynh. Root Apical Meristem. International Journal of Molecular Sciences. 2021; 22 (14):7305.

Chicago/Turabian Style

Leonardo Bruno; Emanuela Talarico; Luz Cabeiras-Freijanes; Maria Madeo; Antonella Muto; Marco Minervino; Luigi Lucini; Begoña Miras-Moreno; Adriano Sofo; Fabrizio Araniti. 2021. "Coumarin Interferes with Polar Auxin Transport Altering Microtubule Cortical Array Organization in Arabidopsis thaliana (L.) Heynh. Root Apical Meristem." International Journal of Molecular Sciences 22, no. 14: 7305.

Journal article
Published: 14 June 2021 in Agronomy
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Plant biostimulants are “green” solutions to improve crop production. Trichoderma spp. and phosphites, ordinarily used as biocontrol agents, can trigger phytostimulation, also promoting endogenous mechanisms involved in plant growth and development. The present study aimed at assessing the efficacy of a phosphite-based formulation (Phosphit-One) and Trichoderma harzianum-T22 on the morpho-physiological response and modulation of the metabolomics profile in zucchini squash plants (Cucurbita pepo L.) cultivated in controlled growth conditions (Fitotron®). The highest values of fresh biomass production (390.9 g plant−1) and root dry weight (5.6 g plant−1) were obtained for Trichoderma-treated plants. This last treatment resulted in an improved physiological performance (SPAD index, CO2 assimilation rate, and Fv/Fm ratio) measured 30 days after transplanting. Both Trichoderma and phosphite treatments induced a broad metabolic reprogramming in leaves, evident also for the phosphite treatment that did not result in a growth promotion. The microbial and the non-microbial treatments showed distinctive signatures in secondary metabolism yet, common responses could be also highlighted. For instance, both Trichoderma and phosphite triggered ROS-mediated signaling processes, together with the accumulation of phenylpropanoids, glucosinolates, and phytoalexins. Furthermore, a significant alteration of phytohormones was observed, with terpenoid gibberellins and brassinosteroids showing the largest differences. The metabolomic signatures induced by Trichoderma and phosphite in zucchini squash provided molecular insights into the processes underlying elicitation of plant defense due to biostimulation. Interestingly, the modulation of plant secondary metabolism by both treatments did not impair plant growth.

ACS Style

Luigi Formisano; Begoña Miras-Moreno; Michele Ciriello; Christophe El-Nakhel; Giandomenico Corrado; Luigi Lucini; Giuseppe Colla; Youssef Rouphael. Trichoderma and Phosphite Elicited Distinctive Secondary Metabolite Signatures in Zucchini Squash Plants. Agronomy 2021, 11, 1205 .

AMA Style

Luigi Formisano, Begoña Miras-Moreno, Michele Ciriello, Christophe El-Nakhel, Giandomenico Corrado, Luigi Lucini, Giuseppe Colla, Youssef Rouphael. Trichoderma and Phosphite Elicited Distinctive Secondary Metabolite Signatures in Zucchini Squash Plants. Agronomy. 2021; 11 (6):1205.

Chicago/Turabian Style

Luigi Formisano; Begoña Miras-Moreno; Michele Ciriello; Christophe El-Nakhel; Giandomenico Corrado; Luigi Lucini; Giuseppe Colla; Youssef Rouphael. 2021. "Trichoderma and Phosphite Elicited Distinctive Secondary Metabolite Signatures in Zucchini Squash Plants." Agronomy 11, no. 6: 1205.

Journal article
Published: 26 May 2021 in Plant Science
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Cyclic Nucleotides Monophosphate (cNMP) are key signalling compounds whose role in plant cell signal transduction is still poorly understood. In this work we used sildenafil, a phosphodiesterase (PDE) inhibitor used in human, to amplify the signal cascade triggered by cNMP using tomato as model plant. Metabolomics was then used, together with plant growth and root architecture parameters, to unravel the changes elicited by PDE inhibition either under non-stress and 100 mM NaCl salinity conditions. The PDE inhibitor elicited a significant increase in biomass (+62 %) and root length (+56 %) under no stress conditions, and affected root architecture in terms of distribution over diameter classes. Together with cGMP, others cNMP were modulated by the treatment. Moreover, PDE inhibition triggered a broad metabolic reprogramming involving photosynthesis and secondary metabolism. A complex crosstalk network of phytohormones and other signalling compounds could be observed in treated plants. Nonetheless, metabolites related to redox imbalance processes and NO signalling could be highlighted in tomato following PDE application. Despite salinity damped down the growth-promoting effects of sildenafil, interesting implications in plant mitigation to stress-related detrimental effects could be observed.

ACS Style

Begoña Miras-Moreno; Leilei Zhang; Biancamaria Senizza; Luigi Lucini. A metabolomics insight into the Cyclic Nucleotide Monophosphate signaling cascade in tomato under non-stress and salinity conditions. Plant Science 2021, 309, 110955 .

AMA Style

Begoña Miras-Moreno, Leilei Zhang, Biancamaria Senizza, Luigi Lucini. A metabolomics insight into the Cyclic Nucleotide Monophosphate signaling cascade in tomato under non-stress and salinity conditions. Plant Science. 2021; 309 ():110955.

Chicago/Turabian Style

Begoña Miras-Moreno; Leilei Zhang; Biancamaria Senizza; Luigi Lucini. 2021. "A metabolomics insight into the Cyclic Nucleotide Monophosphate signaling cascade in tomato under non-stress and salinity conditions." Plant Science 309, no. : 110955.

Journal article
Published: 15 February 2021 in Industrial Crops and Products
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In this work, the combination of elicited plant suspension cultured cells (PSCCs) with untargeted metabolomics establishes a powerful, cutting-edge strategy to unravel the effects of elicitors on the biosynthetic potential of medicinal plants, as Bryophyllum sp. The PSCC technology constitutes a successful biotechnological system for the study and production of bioactive compounds throughout the inclusion of elicitors with the ability to modulate secondary metabolism. The use of methyl jasmonate (MJ) and salicylic acid (SA) as abiotic elicitors on bryophyllum PSCCs, resulted in differential effects on cell growth and secondary metabolism, depending on the species, including synergistic and antagonistic effects. This fact suggests that both elicitors play a pleiotropic effect on plant secondary metabolism, showing complex interactions, according to the UHPLC-QTOF mass spectrometry profiling. Thus, the combination of both elicitors induced a strong synergistic on B. daigremontianum PSCCs, with 2272 putatively annotated compounds, whereas it caused a negative effect on the secondary metabolism of B. × houghtonii PSCCs, being MJ the only elicitor driving a positive effect, presenting 2972 annotated compounds. Meanwhile, B. tubiflorum PSCCs did not show a significant modulation of secondary metabolism, with 1521 annotated compounds. The metabolite annotation indicated that three families of secondary metabolites were mainly affected by elicitation: phenolic compounds constituted the most affected family by elicitation, mainly represented by flavonoids and lignans; N-containing compounds included glucosinolates, amines, and alkaloids, reported to Bryophyllum sp. for the first time; and terpenoids included mainly phytoalexins and saponins. The results depict a deep genotype-dependent metabolomic reprogramming of secondary metabolism in response to elicitors, thanks to the application of untargeted metabolomics. This knowledge will allow the consideration of Bryophyllum sp. as a valuable source of bioactive compounds, with the potential associated to PSCCs, for being included in food, cosmetic, and pharmaceutical applications.

ACS Style

Pascual García-Pérez; Begoña Miras-Moreno; Luigi Lucini; Pedro P. Gallego. The metabolomics reveals intraspecies variability of bioactive compounds in elicited suspension cell cultures of three Bryophyllum species. Industrial Crops and Products 2021, 163, 113322 .

AMA Style

Pascual García-Pérez, Begoña Miras-Moreno, Luigi Lucini, Pedro P. Gallego. The metabolomics reveals intraspecies variability of bioactive compounds in elicited suspension cell cultures of three Bryophyllum species. Industrial Crops and Products. 2021; 163 ():113322.

Chicago/Turabian Style

Pascual García-Pérez; Begoña Miras-Moreno; Luigi Lucini; Pedro P. Gallego. 2021. "The metabolomics reveals intraspecies variability of bioactive compounds in elicited suspension cell cultures of three Bryophyllum species." Industrial Crops and Products 163, no. : 113322.

Journal article
Published: 08 February 2021 in Plants
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Despite the scientific evidence supporting their biostimulant activity, the molecular mechanism(s) underlying the activity of protein hydrolysates (PHs) and the specificity among different products are still poorly explored. This work tested five different protein hydrolysates, produced from different plant sources using the same enzymatic approach, for their ability to promote rooting in tomato cuttings following quick dipping. Provided that all the different PHs increased root length (45–93%) and some of them increased root number (37–56%), untargeted metabolomics followed by multivariate statistics and pathway analysis were used to unravel the molecular processes at the basis of the biostimulant activity. Distinct metabolomic signatures could be found in roots following the PHs treatments. In general, PHs shaped the phytohormone profile, modulating the complex interaction between cytokinins and auxins, an interplay playing a pivotal role in root development, and triggered a down accumulation of brassinosteroids. Concerning secondary metabolism, PHs induced the accumulation of aliphatic glucosinolates, alkaloids, and phenylpropanoids, potentially eliciting crop resilience to stress conditions. Here, we confirm that PHs may have a hormone-like activity, and that their application can modulate plant growth, likely interfering with signaling processes. Noteworthy, the heterogenicity of the botanical origin supported the distinctive and peculiar metabolomic responses we observed across the products tested. While supporting their biostimulant activity, these findings suggest that a generalized crop response to PHs cannot be defined and that specific effects are rather to be investigated.

ACS Style

Angela Ceccarelli; Begoña Miras-Moreno; Valentina Buffagni; Biancamaria Senizza; Youry Pii; Mariateresa Cardarelli; Youssef Rouphael; Giuseppe Colla; Luigi Lucini. Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism. Plants 2021, 10, 326 .

AMA Style

Angela Ceccarelli, Begoña Miras-Moreno, Valentina Buffagni, Biancamaria Senizza, Youry Pii, Mariateresa Cardarelli, Youssef Rouphael, Giuseppe Colla, Luigi Lucini. Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism. Plants. 2021; 10 (2):326.

Chicago/Turabian Style

Angela Ceccarelli; Begoña Miras-Moreno; Valentina Buffagni; Biancamaria Senizza; Youry Pii; Mariateresa Cardarelli; Youssef Rouphael; Giuseppe Colla; Luigi Lucini. 2021. "Foliar Application of Different Vegetal-Derived Protein Hydrolysates Distinctively Modulates Tomato Root Development and Metabolism." Plants 10, no. 2: 326.

Journal article
Published: 05 January 2021 in Plants
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Mineral elements are essential for plant growth and development and strongly affect crop yield and quality. To cope with an everchanging environment, plants have developed specific responses to combined nutrient variations. In this work, we investigated the effects of multifactorial treatments with three macrocations (K, Ca, and Mg) on lettuce (Lactuca sativa L.) varieties that strongly diverge in leaf pigmentation (full red or green). Specifically, we monitored main leaf parameters and metabolomics profiles of hydroponically grown plants fed with isosmotic nutrient solutions that have different proportions of macroelements. The result revealed a high biochemical plasticity of lettuce, significantly affected by the genotype, the nutrient solution, and their interaction. Our work also provided evidence and insights into the different intraspecific responses to multifactorial variation of macrocations, with two varieties having distinct strategies to metabolically respond to nutrient variation. Overall, plant adaptive mechanisms increased the phytochemical diversity between the varieties both among and within the main classes of plant secondary metabolites. Finally, our work also implies that the interaction of a pre-existing phytochemical diversity with the management of multiple mineral elements can offer added health-related benefits to the edible product specific to the variety.

ACS Style

Giandomenico Corrado; Luigi Lucini; Begoña Miras-Moreno; Leilei Zhang; Christophe El-Nakhel; Giuseppe Colla; Youssef Rouphael. Intraspecific Variability Largely Affects the Leaf Metabolomics Response to Isosmotic Macrocation Variations in Two Divergent Lettuce (Lactuca sativa L.) Varieties. Plants 2021, 10, 91 .

AMA Style

Giandomenico Corrado, Luigi Lucini, Begoña Miras-Moreno, Leilei Zhang, Christophe El-Nakhel, Giuseppe Colla, Youssef Rouphael. Intraspecific Variability Largely Affects the Leaf Metabolomics Response to Isosmotic Macrocation Variations in Two Divergent Lettuce (Lactuca sativa L.) Varieties. Plants. 2021; 10 (1):91.

Chicago/Turabian Style

Giandomenico Corrado; Luigi Lucini; Begoña Miras-Moreno; Leilei Zhang; Christophe El-Nakhel; Giuseppe Colla; Youssef Rouphael. 2021. "Intraspecific Variability Largely Affects the Leaf Metabolomics Response to Isosmotic Macrocation Variations in Two Divergent Lettuce (Lactuca sativa L.) Varieties." Plants 10, no. 1: 91.

Data descriptor
Published: 15 December 2020 in Data
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The study of the relationship between cultivated plants and environmental factors can provide information ranging from a deeper understanding of the plant biological system to the development of more effective management strategies for improving yield, quality, and sustainability of the produce. In this article, we present a comprehensive metabolomics dataset of two phytochemically divergent lettuce (Lactuca sativa L.) butterhead varieties under different growing conditions. Plants were cultivated in hydroponics in a growth chamber with ambient control. The pre-harvest factors that were independently investigated were light intensity (two levels), the ionic strength of the nutrient solutions (three levels), and the molar ratio of three macroelements (K, Mg, and Ca) in the nutrient solution (three levels). We used an untargeted, mass-spectrometry-based approach to characterize the metabolomics profiles of leaves harvested 19 days after transplant. The data revealed the ample impact on both primary and secondary metabolism and its range of variation. Moreover, our dataset is useful for uncovering the complex effects of the genotype, the environmental factor(s), and their interaction, which may deserve further investigation.

ACS Style

Giandomenico Corrado; Luigi Lucini; Begoña Miras-Moreno; Leilei Zhang; Biancamaria Senizza; Boris Basile; Youssef Rouphael. Dataset on the Effects of Different Pre-Harvest Factors on the Metabolomics Profile of Lettuce (Lactuca sativa L.) Leaves. Data 2020, 5, 119 .

AMA Style

Giandomenico Corrado, Luigi Lucini, Begoña Miras-Moreno, Leilei Zhang, Biancamaria Senizza, Boris Basile, Youssef Rouphael. Dataset on the Effects of Different Pre-Harvest Factors on the Metabolomics Profile of Lettuce (Lactuca sativa L.) Leaves. Data. 2020; 5 (4):119.

Chicago/Turabian Style

Giandomenico Corrado; Luigi Lucini; Begoña Miras-Moreno; Leilei Zhang; Biancamaria Senizza; Boris Basile; Youssef Rouphael. 2020. "Dataset on the Effects of Different Pre-Harvest Factors on the Metabolomics Profile of Lettuce (Lactuca sativa L.) Leaves." Data 5, no. 4: 119.

Journal article
Published: 02 September 2020 in International Journal of Molecular Sciences
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Sub-optimal growing conditions have a major effect on plants; therefore, large efforts are devoted to maximizing the availability of agricultural inputs to crops. To increase the sustainable use of non-renewable inputs, attention is currently given to the study of plants under non-optimal conditions. In this work, we investigated the impact of sub-optimal macrocations availability and light intensity in two lettuce varieties that differ for the accumulation of secondary metabolites (i.e., ‘Red Salanova’ and ‘Green Salanova’). Photosynthesis-related measurements and untargeted metabolomics were used to identify responses and pathways involved in stress resilience. The pigmented (‘Red’) and the non-pigmented (‘Green Salanova’) lettuce exhibited distinctive responses to sub-optimal conditions. The cultivar specific metabolomic signatures comprised a broad modulation of metabolism, including secondary metabolites, phytohormones, and membrane lipids signaling cascade. Several stress-related metabolites were altered by either treatment, including polyamines (and other nitrogen-containing compounds), phenylpropanoids, and lipids. The metabolomics and physiological response to macrocations availability and light intensity also implies that the effects of low-input sustainable farming systems should be evaluated considering a range of cultivar-specific positive and disadvantageous metabolic effects in addition to yield and other socio-economic parameters.

ACS Style

Begoña Miras-Moreno; Giandomenico Corrado; Leilei Zhang; Biancamaria Senizza; Laura Righetti; Renato Bruni; Christophe El-Nakhel; Maria Isabella Sifola; Antonio Pannico; Stefania De Pascale; Youssef Rouphael; Luigi Lucini. The Metabolic Reprogramming Induced by Sub-optimal Nutritional and Light Inputs in Soilless Cultivated Green and Red Butterhead Lettuce. International Journal of Molecular Sciences 2020, 21, 6381 .

AMA Style

Begoña Miras-Moreno, Giandomenico Corrado, Leilei Zhang, Biancamaria Senizza, Laura Righetti, Renato Bruni, Christophe El-Nakhel, Maria Isabella Sifola, Antonio Pannico, Stefania De Pascale, Youssef Rouphael, Luigi Lucini. The Metabolic Reprogramming Induced by Sub-optimal Nutritional and Light Inputs in Soilless Cultivated Green and Red Butterhead Lettuce. International Journal of Molecular Sciences. 2020; 21 (17):6381.

Chicago/Turabian Style

Begoña Miras-Moreno; Giandomenico Corrado; Leilei Zhang; Biancamaria Senizza; Laura Righetti; Renato Bruni; Christophe El-Nakhel; Maria Isabella Sifola; Antonio Pannico; Stefania De Pascale; Youssef Rouphael; Luigi Lucini. 2020. "The Metabolic Reprogramming Induced by Sub-optimal Nutritional and Light Inputs in Soilless Cultivated Green and Red Butterhead Lettuce." International Journal of Molecular Sciences 21, no. 17: 6381.

Journal article
Published: 21 August 2020 in Foods
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Considering that functional components of plant foods are mainly secondary-metabolism products, we investigated the shaping of health-promoting compounds in hydroponically grown butterhead lettuce (Lactuca sativa L. var. capitata) as a function of the strength of the nutrient solution utilized. To this aim, untargeted metabolomics profiling, in vitro antioxidant capacity (total phenolics, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP) assays), and the inhibition of selected enzyme activities were investigated in two butterhead lettuce cultivars with different pigmentation, i.e., green and red Salanova. Full-strength nutrition, together with half- and quarter-strength solutions of macronutrients, was tested. Our results indicate that by reducing the nutrients strength, we could elicit a distinctive shaping of the phenolic profile of lettuce. It is noteworthy that only specific classes of phenolics (namely, lignans and phenolic acids, followed by flavones and anthocyanins) were modulated by the induction of nutritional eustress (fold-change values in the range between −5 and +11). This indicates that specific responses, rather than a generalized induction of phenolic compounds, could be observed. Nonetheless, a genotype-dependent response could be observed, with the red cultivar being much more responsive to nutritional deprivation than the green Salanova lettuce. Indeed, analysis of variance (ANOVA) confirmed a genotype x nutrition interaction in red Salanova (p < 0.001). As a consequence of the changes in phenolic composition, also the antioxidant capacity (p < 0.001) and amylase inhibition (p < 0.001) properties were affected by the growing conditions. However, the effect on cholinesterase and tyrosinase inhibition was poorly affected by the nutritional strength. Provided that yields are not compromised, the application of a controlled nutritional eustress in hydroponically cultivated lettuce may represent a valuable strategy to produce food with tailored functional features in a sustainable manner.

ACS Style

Biancamaria Senizza; Leilei Zhang; Begoña Miras-Moreno; Laura Righetti; Gokhan Zengin; Gunes Ak; Renato Bruni; Luigi Lucini; Maria Isabella Sifola; Christophe El-Nakhel; Giandomenico Corrado; Youssef Rouphael. The Strength of the Nutrient Solution Modulates the Functional Profile of Hydroponically Grown Lettuce in a Genotype-Dependent Manner. Foods 2020, 9, 1156 .

AMA Style

Biancamaria Senizza, Leilei Zhang, Begoña Miras-Moreno, Laura Righetti, Gokhan Zengin, Gunes Ak, Renato Bruni, Luigi Lucini, Maria Isabella Sifola, Christophe El-Nakhel, Giandomenico Corrado, Youssef Rouphael. The Strength of the Nutrient Solution Modulates the Functional Profile of Hydroponically Grown Lettuce in a Genotype-Dependent Manner. Foods. 2020; 9 (9):1156.

Chicago/Turabian Style

Biancamaria Senizza; Leilei Zhang; Begoña Miras-Moreno; Laura Righetti; Gokhan Zengin; Gunes Ak; Renato Bruni; Luigi Lucini; Maria Isabella Sifola; Christophe El-Nakhel; Giandomenico Corrado; Youssef Rouphael. 2020. "The Strength of the Nutrient Solution Modulates the Functional Profile of Hydroponically Grown Lettuce in a Genotype-Dependent Manner." Foods 9, no. 9: 1156.

Journal article
Published: 05 August 2020 in Food Chemistry
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UV-B-driven modulation of secondary metabolism in peach fruit by enhancing the biosynthesis of specific phenolic subclasses, is attracting interest among consumers. However, current literature explored the UV-B-induced metabolic changes only in peach skin subjected to direct UV-B irradiation. Accordingly, this study aimed to understand whether UV-B radiation penetrates the fruit skin and is able to induce metabolic changes also within the inner flesh. Peaches were UV-B-irradiated either 10 or 60 min, and the flesh was sampled after 24 and 36 h. Non-targeted metabolomics revealed that UV-B has a strong impact on peach flesh metabolome, determining an initial decrease after 24 h, followed by an overall increase after 36 h, particularly for terpenoids, phenylpropanoids, phytoalexins and fatty acids in the 60 min UV-B-treated samples (+150.02, +99.14, +43.79 and +25.44 log2FC, respectively). Transmittance analysis indicated that UV-B radiation does not penetrate below the skin, suggesting a possible signalling pathway between tissues.

ACS Style

Marco Santin; Annamaria Ranieri; Marie-Theres Hauser; Begoña Miras-Moreno; Gabriele Rocchetti; Luigi Lucini; Åke Strid; Antonella Castagna. The outer influences the inner: Postharvest UV-B irradiation modulates peach flesh metabolome although shielded by the skin. Food Chemistry 2020, 338, 127782 .

AMA Style

Marco Santin, Annamaria Ranieri, Marie-Theres Hauser, Begoña Miras-Moreno, Gabriele Rocchetti, Luigi Lucini, Åke Strid, Antonella Castagna. The outer influences the inner: Postharvest UV-B irradiation modulates peach flesh metabolome although shielded by the skin. Food Chemistry. 2020; 338 ():127782.

Chicago/Turabian Style

Marco Santin; Annamaria Ranieri; Marie-Theres Hauser; Begoña Miras-Moreno; Gabriele Rocchetti; Luigi Lucini; Åke Strid; Antonella Castagna. 2020. "The outer influences the inner: Postharvest UV-B irradiation modulates peach flesh metabolome although shielded by the skin." Food Chemistry 338, no. : 127782.

Journal article
Published: 11 June 2020 in Agronomy
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Sweet basil (Ocimum basilicum L.) is an aromatic, tender leafy green cultivated for the fresh market and industrial processing. Like many other leafy vegetables, this species can be harvested more than once to increase productivity. Although the cut-and-come-again (CC) harvest strategy is widespread in basil, not much is known about the effect of the cut. In this work, we examined differences in biomass, physiology, nutrient use efficiency and antioxidant capacity of basil leaves from two successive harvests. Moreover, we also performed an untargeted metabolomics analysis to reveal variations in the leaf metabolite profile. The data indicated that the cut affected many of the parameters under investigation, including a modest reduction of yield (−20%), of nitrogen use efficiency (−21%) and of some quality attributes such as the antioxidant capacity (−16%) and the nitrate content in leaves (+48%). Moreover, leaves of successive cuts displayed a significant variation of the profile of bioactive compounds, characterized by an overall decrease of phenylpropanoids and an increase in terpenoids. Our data revealed the impact of CC harvesting strategy in basil, and that this strategy offers the possibility to yield leaves with different metabolomics profiles and quality attributes.

ACS Style

Giandomenico Corrado; Pasquale Chiaiese; Luigi Lucini; Begoña Miras-Moreno; Giuseppe Colla; Youssef Rouphael. Successive Harvests Affect Yield, Quality and Metabolic Profile of Sweet Basil (Ocimum basilicum L.). Agronomy 2020, 10, 830 .

AMA Style

Giandomenico Corrado, Pasquale Chiaiese, Luigi Lucini, Begoña Miras-Moreno, Giuseppe Colla, Youssef Rouphael. Successive Harvests Affect Yield, Quality and Metabolic Profile of Sweet Basil (Ocimum basilicum L.). Agronomy. 2020; 10 (6):830.

Chicago/Turabian Style

Giandomenico Corrado; Pasquale Chiaiese; Luigi Lucini; Begoña Miras-Moreno; Giuseppe Colla; Youssef Rouphael. 2020. "Successive Harvests Affect Yield, Quality and Metabolic Profile of Sweet Basil (Ocimum basilicum L.)." Agronomy 10, no. 6: 830.

Journal article
Published: 05 June 2020 in International Journal of Molecular Sciences
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Fe chlorosis is considered as one of the major constraints on crop growth and yield worldwide, being particularly worse when associated with S shortage, due to the tight link between Fe and S. Plant adaptation to inadequate nutrient availabilities often relies on the release of root exudates that enhance nutrients, mobilization from soil colloids and favour their uptake by roots. This work aims at characterizing the exudomic profile of hydroponically grown tomato plants subjected to either single or combined Fe and S deficiency, as well as at shedding light on the regulation mechanisms underlying Fe and S acquisition processes by plants. Root exudates have been analysed by untargeted metabolomics, through liquid chromatography–mass spectrometry as well as gas chromatography–mass spectrometry following derivatization. More than 200 metabolites could be putatively annotated. Venn diagrams show that 23%, 10% and 21% of differential metabolites are distinctively modulated by single Fe deficiency, single S deficiency or combined Fe–S deficiency, respectively. Interestingly, for the first time, a mugineic acid derivative is detected in dicot plants root exudates. The results seem to support the hypothesis of the co-existence of the two Fe acquisition strategies in tomato plants.

ACS Style

Stefania Astolfi; Youry Pii; Tanja Mimmo; Luigi Lucini; Maria B. Miras-Moreno; Eleonora Coppa; Simona Violino; Silvia Celletti; Stefano Cesco. Single and Combined Fe and S Deficiency Differentially Modulate Root Exudate Composition in Tomato: A Double Strategy for Fe Acquisition? International Journal of Molecular Sciences 2020, 21, 4038 .

AMA Style

Stefania Astolfi, Youry Pii, Tanja Mimmo, Luigi Lucini, Maria B. Miras-Moreno, Eleonora Coppa, Simona Violino, Silvia Celletti, Stefano Cesco. Single and Combined Fe and S Deficiency Differentially Modulate Root Exudate Composition in Tomato: A Double Strategy for Fe Acquisition? International Journal of Molecular Sciences. 2020; 21 (11):4038.

Chicago/Turabian Style

Stefania Astolfi; Youry Pii; Tanja Mimmo; Luigi Lucini; Maria B. Miras-Moreno; Eleonora Coppa; Simona Violino; Silvia Celletti; Stefano Cesco. 2020. "Single and Combined Fe and S Deficiency Differentially Modulate Root Exudate Composition in Tomato: A Double Strategy for Fe Acquisition?" International Journal of Molecular Sciences 21, no. 11: 4038.

Journal article
Published: 03 April 2020 in International Journal of Molecular Sciences
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Sweet basil (Ocimum basilicum L.) is a highly versatile and globally popular culinary herb, and a rich source of aromatic and bioactive compounds. Particularly for leafy vegetables, nutrient management allows a more efficient and sustainable improvement of crop yield and quality. In this work, we investigated the effects of balanced modulation of the concentration of two antagonist anions (nitrate and chlorine) in basil. Specifically, we evaluated the changes in yield and leaf metabolic profiles in response to four different NO3−:Cl− ratios in two consecutive harvests, using a full factorial design. Our work indicated that the variation of the nitrate-chloride ratio exerts a large effect on both metabolomic profile and yield in basil, which cannot be fully explained only by an anion-anion antagonist outcome. The metabolomic reprogramming involved different biochemical classes of compounds, with distinctive traits as a function of the different nutrient ratios. Such changes involved not only a response to nutrients availability, but also to redox imbalance and oxidative stress. A network of signaling compounds, including NO and phytohormones, underlined the modeling of metabolomic signatures. Our work highlighted the potential and the magnitude of the effect of nutrient solution management in basil and provided an advancement towards understanding the metabolic response to anion antagonism in plants.

ACS Style

Giandomenico Corrado; Luigi Lucini; Begoña Miras-Moreno; Pasquale Chiaiese; Giuseppe Colla; Stefania De Pascale; Youssef Rouphael. Metabolic Insights into the Anion-Anion Antagonism in Sweet Basil: Effects of Different Nitrate/Chloride Ratios in the Nutrient Solution. International Journal of Molecular Sciences 2020, 21, 2482 .

AMA Style

Giandomenico Corrado, Luigi Lucini, Begoña Miras-Moreno, Pasquale Chiaiese, Giuseppe Colla, Stefania De Pascale, Youssef Rouphael. Metabolic Insights into the Anion-Anion Antagonism in Sweet Basil: Effects of Different Nitrate/Chloride Ratios in the Nutrient Solution. International Journal of Molecular Sciences. 2020; 21 (7):2482.

Chicago/Turabian Style

Giandomenico Corrado; Luigi Lucini; Begoña Miras-Moreno; Pasquale Chiaiese; Giuseppe Colla; Stefania De Pascale; Youssef Rouphael. 2020. "Metabolic Insights into the Anion-Anion Antagonism in Sweet Basil: Effects of Different Nitrate/Chloride Ratios in the Nutrient Solution." International Journal of Molecular Sciences 21, no. 7: 2482.

Journal article
Published: 10 February 2020 in Plants
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Plant resistance to salinity stress is one of the main challenges of agriculture. The comprehension of the molecular and cellular mechanisms involved in plant tolerance to salinity can help to contrast crop losses due to high salt conditions in soil. In this study, Salicornia brachiata and Suaeda maritima, two plants with capacity to adapt to high salinity levels, were investigated at proteome level to highlight the key processes involved in their tolerance to NaCl. With this purpose, plants were treated with 200 mM NaCl as optimal concentration and 500 mM NaCl as a moderate stressing concentration for 14 days. Indeed, 200 mM NaCl did not result in an evident stress condition for both species, although photosynthesis was affected (with a general up accumulation of photosynthesis-related proteins in S. brachiata under salinity). Our findings indicate a coordinated response to salinity in both the halophytes considered, under NaCl conditions. In addition to photosynthesis, heat shock proteins and peroxidase, expansins, signaling processes, and modulation of transcription/translation were affected by salinity. Interestingly, our results suggested distinct mechanisms of tolerance to salinity between the two species considered, with S. brachiata likely having a more efficient mechanism of response to NaCl.

ACS Style

Jenifer Joseph Benjamin; Begoña Miras-Moreno; Fabrizio Araniti; Hajar Salehi; Letizia Bernardo; Ajay Parida; Luigi Lucini. Proteomics Revealed Distinct Responses to Salinity between the Halophytes Suaeda maritima (L.) Dumort and Salicornia brachiata (Roxb). Plants 2020, 9, 227 .

AMA Style

Jenifer Joseph Benjamin, Begoña Miras-Moreno, Fabrizio Araniti, Hajar Salehi, Letizia Bernardo, Ajay Parida, Luigi Lucini. Proteomics Revealed Distinct Responses to Salinity between the Halophytes Suaeda maritima (L.) Dumort and Salicornia brachiata (Roxb). Plants. 2020; 9 (2):227.

Chicago/Turabian Style

Jenifer Joseph Benjamin; Begoña Miras-Moreno; Fabrizio Araniti; Hajar Salehi; Letizia Bernardo; Ajay Parida; Luigi Lucini. 2020. "Proteomics Revealed Distinct Responses to Salinity between the Halophytes Suaeda maritima (L.) Dumort and Salicornia brachiata (Roxb)." Plants 9, no. 2: 227.

Journal article
Published: 09 December 2019 in Antioxidants
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Wild edible plants have been used in cooking since ancient times. Recently, their value has improved as a result of the scientific evidence for their nutraceutical properties. Sanguisorba minor Scop. (salad burnet) plants were hydroponically grown and two consecutive cuts took place at 15 (C1) and 30 (C2) days after sowing. An untargeted metabolomics approach was utilized to fingerprint phenolics and other health-related compounds in this species; this approach revealed the different effects of the two cuts on the plant. S. minor showed a different and complex secondary metabolite profile, which was influenced by the cut. In fact, flavonoids increased in leaves obtained from C2, especially flavones. However, other secondary metabolites were downregulated in leaves from C2 compared to those detected in leaves from C1, as evidenced by the combination of the variable important in projections (VIP score > 1.3) and the fold-change (FC > 2). The storage of S. minor leaves for 15 days as fresh-cut products did not induce significant changes in the phenolic content and antioxidant capacity, which indicates that the nutraceutical value was maintained. The only difference evidenced during storage was that leaves obtained from C2 showed a lower constitutive content of nutraceutical compounds than leaves obtained from C1; except for chlorophylls and carotenoids. In conclusion, the cut was the main influence on the modulation of secondary metabolites in leaves, and the effects were independent of storage.

ACS Style

Costanza Ceccanti; Marco Landi; Gabriele Rocchetti; Maria Begoña Miras Moreno; Luigi Lucini; Luca Incrocci; Alberto Pardossi; Lucia Guidi. Hydroponically Grown Sanguisorba minor Scop.: Effects of Cut and Storage on Fresh-Cut Produce. Antioxidants 2019, 8, 631 .

AMA Style

Costanza Ceccanti, Marco Landi, Gabriele Rocchetti, Maria Begoña Miras Moreno, Luigi Lucini, Luca Incrocci, Alberto Pardossi, Lucia Guidi. Hydroponically Grown Sanguisorba minor Scop.: Effects of Cut and Storage on Fresh-Cut Produce. Antioxidants. 2019; 8 (12):631.

Chicago/Turabian Style

Costanza Ceccanti; Marco Landi; Gabriele Rocchetti; Maria Begoña Miras Moreno; Luigi Lucini; Luca Incrocci; Alberto Pardossi; Lucia Guidi. 2019. "Hydroponically Grown Sanguisorba minor Scop.: Effects of Cut and Storage on Fresh-Cut Produce." Antioxidants 8, no. 12: 631.

Original research article
Published: 03 May 2019 in Frontiers in Plant Science
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Plant-derived protein hydrolysates (PHs) are an important category of biostimulants able to increase plant growth and crop yield especially under environmental stress conditions. PHs can be applied as foliar spray or soil drench. Foliar spray is generally applied to achieve a relatively short-term response, whereas soil drench is used when a long-term effect is desired. The aim of the study was to elucidate the biostimulant action of PH application method (foliar spray or substrate drench) on morpho-physiological traits and metabolic profile of tomato grown under limited water availability. An untreated control was also included. A high-throughput image-based phenotyping (HTP) approach was used to non-destructively monitor the crop response under limited water availability (40% of container capacity) in a controlled environment. Moreover, metabolic profile of leaves was determined at the end of the trial. Dry biomass of shoots at the end of the trial was significantly correlated with number of green pixels (R2 = 0.90) and projected shoot area, respectively. Both drench and foliar treatments had a positive impact on the digital biomass compared to control while the photosynthetic performance of the plants was slightly influenced by treatments. Overall drench application under limited water availability more positively influenced biomass accumulation and metabolic profile than foliar application. Significantly higher transpiration use efficiency was observed with PH-drench applications indicating better stomatal conductance. The mass-spectrometry based metabolomic analysis allowed the identification of distinct biochemical signatures in PH-treated plants. Metabolomic changes involved a wide and organized range of biochemical processes that included, among others, phytohormones (notably a decrease in cytokinins and an accumulation of salicylates) and lipids (including membrane lipids, sterols, and terpenes). From a general perspective, treated tomato plants exhibited an improved tolerance to reactive oxygen species (ROS)-mediated oxidative imbalance. Such capability to cope with oxidative stress might have resulted from a coordinated action of signaling compounds (salicylic acid and hydroxycinnamic amides), radical scavengers such as carotenoids and prenyl quinones, as well as a reduced biosynthesis of tetrapyrrole coproporphyrins.

ACS Style

Kenny Paul; Mirella Sorrentino; Luigi Lucini; Youssef Rouphael; Mariateresa Cardarelli; Paolo Bonini; Begoña Miras-Moreno; Hélène Reynaud; Renaud Canaguier; Martin Trtílek; Klara Panzarova; Giuseppe Colla. A Combined Phenotypic and Metabolomic Approach for Elucidating the Biostimulant Action of a Plant-Derived Protein Hydrolysate on Tomato Grown Under Limited Water Availability. Frontiers in Plant Science 2019, 10, 1 .

AMA Style

Kenny Paul, Mirella Sorrentino, Luigi Lucini, Youssef Rouphael, Mariateresa Cardarelli, Paolo Bonini, Begoña Miras-Moreno, Hélène Reynaud, Renaud Canaguier, Martin Trtílek, Klara Panzarova, Giuseppe Colla. A Combined Phenotypic and Metabolomic Approach for Elucidating the Biostimulant Action of a Plant-Derived Protein Hydrolysate on Tomato Grown Under Limited Water Availability. Frontiers in Plant Science. 2019; 10 ():1.

Chicago/Turabian Style

Kenny Paul; Mirella Sorrentino; Luigi Lucini; Youssef Rouphael; Mariateresa Cardarelli; Paolo Bonini; Begoña Miras-Moreno; Hélène Reynaud; Renaud Canaguier; Martin Trtílek; Klara Panzarova; Giuseppe Colla. 2019. "A Combined Phenotypic and Metabolomic Approach for Elucidating the Biostimulant Action of a Plant-Derived Protein Hydrolysate on Tomato Grown Under Limited Water Availability." Frontiers in Plant Science 10, no. : 1.

Article
Published: 19 January 2019 in Euphytica
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A better understanding of the plant metabolites produced in response to disease infection may be useful for the development of disease-resistant crop varieties. In the present study, ultra high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (QTOF-MS) was used to identify differentially accumulated metabolites in a subset of sugar beet genotypes harbouring different levels of resistance to Cercospora leaf spot (CLS), a disease caused by the fungal pathogen Cercospora beticola. Leaves of three susceptible (S1, S2 and S3) and two resistant (R1 and R2) genotypes were subjected to QTOF-MS for metabolite profiling. A wide range of metabolites was identified in sugar beet genotypes using metabolomics. Results of Partial Least Squares-Discriminant Analysis indicated that 15 metabolites could better discriminate resistant and susceptible genotypes. A Volcano Plot analysis indicated that the flavonoid quercetin 3-O-(6″-O-p-coumaroyl)-glucoside and gibberellin A51 with the highest absolute fold change (FC = 16), were repressed in resistant samples. Among the 3 metabolites (isovitexin-7-O-xyloside, 3-demethylubiquinol-8 and apigenin 7-O-d-glucoside) showing significant up accumulation in CLS-resistant samples, the flavonoid isovitexin-7-O-xyloside (FC = 4825.634) is associated with resistance to infection with fungal species causing the disease in other crops. Although further studies are still necessary to better elucidate the mechanism of resistance, our results suggest that breeders might exclude susceptible plants based on discriminating metabolites without the need for field inoculation tests. The results also create a solid basis for metabolite-associated reverse genetics and single nucleotide polymorphism discovery based on significantly differentially accumulated metabolites, whose identification is a next strategic priority. The results obtained also underline the role of metabolic signature in CLS resistance mechanisms and provide a platform for the metabolic engineering of sugar beet with higher resistance against C. beticola pathogen.

ACS Style

Bahram Heidari; Begoña Miras-Moreno; Luigi Lucini; Melvin Bolton; Mitchell J. McGrath; Chiara Broccanello; Ilaria Alberti; Luca Sella; Giuseppe Concheri; Andrea Squartini; Massimo Cagnin; Mahdi Hassani; Alessandro Romano; Piergiorgio Stevanato. Mass spectrometry-based metabolomic discrimination of Cercospora leaf spot resistant and susceptible sugar beet germplasms. Euphytica 2019, 215, 25 .

AMA Style

Bahram Heidari, Begoña Miras-Moreno, Luigi Lucini, Melvin Bolton, Mitchell J. McGrath, Chiara Broccanello, Ilaria Alberti, Luca Sella, Giuseppe Concheri, Andrea Squartini, Massimo Cagnin, Mahdi Hassani, Alessandro Romano, Piergiorgio Stevanato. Mass spectrometry-based metabolomic discrimination of Cercospora leaf spot resistant and susceptible sugar beet germplasms. Euphytica. 2019; 215 (2):25.

Chicago/Turabian Style

Bahram Heidari; Begoña Miras-Moreno; Luigi Lucini; Melvin Bolton; Mitchell J. McGrath; Chiara Broccanello; Ilaria Alberti; Luca Sella; Giuseppe Concheri; Andrea Squartini; Massimo Cagnin; Mahdi Hassani; Alessandro Romano; Piergiorgio Stevanato. 2019. "Mass spectrometry-based metabolomic discrimination of Cercospora leaf spot resistant and susceptible sugar beet germplasms." Euphytica 215, no. 2: 25.