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Mushrooms are a good source of protein, dietary fibre, vitamins, minerals and phenolic compounds. However, mushrooms are a very perishable food and the implementation of preservation methods is essential to extend their shelf-life. The preservation methods for mushrooms can be classified into three categories: thermal (drying/freezing), chemical (edible coatings, films and washing solutions) and physical (packing, irradiation, pulsed electric field and ultrasound) processes. These processes can change the nutritional value and bioactive properties of this commodity. The goal of this review is to critically update and discuss the existing information about the effect of postharvest preservation methods on the nutritional value and bioactive properties of edible mushrooms. Drying, especially when high temperatures are applied, can cause the degradation of polysaccharides, proteins and flavour compounds. Freezing is one of the best methods to extend mushrooms’ shelf life but cause the loss of vitamins. Edible coatings and films improve the total sugar, ascorbic acid and bioactive compounds preservation during the storage period. Washing solutions decrease amino acids content. Gamma and electron-beam irradiation decrease the unsaturated fatty acid content, whereas UV-B irradiation significantly increases the vitamin D content. However, there is still limited information about the impact of chemical processes, packaging, pulsed electric field and ultrasound on the nutritional composition and bioactive properties of mushrooms, opening research opportunities for future. This review presents technological and economic alternatives that may support the mushroom processing industries to obtain value-added edible mushrooms and related products.
Sara Marçal; Ana Sofia Sousa; Oludemi Taofiq; Filipa Antunes; Alcina M.M.B. Morais; Ana Cristina Freitas; Lillian Barros; Isabel C.F.R. Ferreira; Manuela Pintado. Impact of postharvest preservation methods on nutritional value and bioactive properties of mushrooms. Trends in Food Science & Technology 2021, 110, 418 -431.
AMA StyleSara Marçal, Ana Sofia Sousa, Oludemi Taofiq, Filipa Antunes, Alcina M.M.B. Morais, Ana Cristina Freitas, Lillian Barros, Isabel C.F.R. Ferreira, Manuela Pintado. Impact of postharvest preservation methods on nutritional value and bioactive properties of mushrooms. Trends in Food Science & Technology. 2021; 110 ():418-431.
Chicago/Turabian StyleSara Marçal; Ana Sofia Sousa; Oludemi Taofiq; Filipa Antunes; Alcina M.M.B. Morais; Ana Cristina Freitas; Lillian Barros; Isabel C.F.R. Ferreira; Manuela Pintado. 2021. "Impact of postharvest preservation methods on nutritional value and bioactive properties of mushrooms." Trends in Food Science & Technology 110, no. : 418-431.
This study aimed to develop new canned chub mackerel products incorporating edible seaweeds (Ascophyllum nodosum, Fucus spiralis, Saccorhiza polyschides, Chondrus crispus, Porphyra sp. and Ulva sp.) harvested in the Portuguese North-Central coast, with simultaneous sensory improvement and minerals enrichment. Two processes were compared, namely the addition of seaweeds in i) the canning step and ii) in the brining step (as the replacement for salt). The concentrations of four macrominerals (Na, K, Ca and Mg), chloride, and twelve trace elements (Co, Cu, Fe, I, Li, Mn, Mo, Rb, Se, Sr, V and Zn) were determined by high-resolution continuum source flame atomic absorption spectrometry (HR-CS-FAAS) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. Results showed that canned chub mackerel incorporating C. crispus and F. spiralis was found to be the preferred sensory option, also exhibiting contents enriched with Cl, Co, Cu, Fe, I, Li, Mg, Mn, Mo, Na, Rb, Se, and Sr. This effect was more pronounced when both seaweed species were added to replace the salt added in the brining step.
Elsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules 2020, 25, 1133 .
AMA StyleElsa F. Vieira, Cristina Soares, Susana Machado, M. Teresa Oliva-Teles, Manuela Correia, Maria João Ramalhosa, Ana Carvalho, Valentina F. Domingues, Filipa Antunes, Simone Morais, Cristina Delerue-Matos. Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition. Molecules. 2020; 25 (5):1133.
Chicago/Turabian StyleElsa F. Vieira; Cristina Soares; Susana Machado; M. Teresa Oliva-Teles; Manuela Correia; Maria João Ramalhosa; Ana Carvalho; Valentina F. Domingues; Filipa Antunes; Simone Morais; Cristina Delerue-Matos. 2020. "Development of New Canned Chub Mackerel Products Incorporating Edible Seaweeds—Influence on the Minerals and Trace Elements Composition." Molecules 25, no. 5: 1133.
The total protein content and the (total and free) amino acid composition of nine edible species of red, brown and green seaweeds collected in the Portuguese North-Central coast were quantified to assess their potential contribution to the recommended dietary intake. Whenever possible, the protein and amino acid composition was compared with that of commercial European seaweeds. The protein content was the highest (P<0.05) in red species (19.1-28.2 g/100g dw), followed by the green seaweed Ulva spp. (20.5-23.3 g/100g dw), with the lowest content found in brown seaweeds (6.90-19.5 g/100g dw). Brown seaweeds presented the lowest mean contents of essential amino acids (EAAs) (41.0% protein) but significantly (P<0.05) higher concentrations of non-essential amino acids (36.1% protein) and free amino acids (6.47-24.0% protein). Tryptophan, methionine and leucine were the limiting EAAs in all species. In contrast, lysine was found in high concentrations, especially in red (2.71-3.85% protein) and green (2.84-4.24% protein) seaweeds.
Elsa Ferreira Vieira; Cristina Soares; Susana Machado; Manuela Correia; Maria João Ramalhosa; Maria Teresa Oliva-Teles; Ana Carvalho; Valentina Domingues; Filipa Antunes; Teresa Azevedo Cardoso Oliveira; Simone Morais; Cristina Delerue-Matos. Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile. Food Chemistry 2018, 269, 264 -275.
AMA StyleElsa Ferreira Vieira, Cristina Soares, Susana Machado, Manuela Correia, Maria João Ramalhosa, Maria Teresa Oliva-Teles, Ana Carvalho, Valentina Domingues, Filipa Antunes, Teresa Azevedo Cardoso Oliveira, Simone Morais, Cristina Delerue-Matos. Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile. Food Chemistry. 2018; 269 ():264-275.
Chicago/Turabian StyleElsa Ferreira Vieira; Cristina Soares; Susana Machado; Manuela Correia; Maria João Ramalhosa; Maria Teresa Oliva-Teles; Ana Carvalho; Valentina Domingues; Filipa Antunes; Teresa Azevedo Cardoso Oliveira; Simone Morais; Cristina Delerue-Matos. 2018. "Seaweeds from the Portuguese coast as a source of proteinaceous material: Total and free amino acid composition profile." Food Chemistry 269, no. : 264-275.
The persistence of bacteria in Anodonta cygnea largely depends on the hemolymph bactericidal activity against aquatic micro-organisms. The aim of this study was to assess the in vitro bactericidal activity of whole hemolymph against multi-drug-resistant Escherichia coli and enterococci strains. Four mussels were injected with multi-resistant strains of E. coli in order to determine their in vivo bactericidal reactivity. In vitro experiments showed that the hemocytes viability decreased almost 70% in 4 h, the same happened in the control hemolymph. Enterococci tested in in vitro experiments were more susceptible than E. coli to whole hemolymph and hemocytes fractions. None of the enterococci bacteria was detected in the hemolymph fractions from 24 to 72 h, while E. coli was still detectable 72 h after inoculation both in the control and respective plasma fractions. The microbial inhibition on both hemolymph and cell pellets suggest that hemocytes may be the main responsibles for that process. In vivo experiments showed that hemolymph inhibited the E. coli load injected and it was correlated with a high tendency for the increase in hemocytes counts, after 72 h. High adherence of E. coli and an intense pseudopods manifestation, mainly to the granulocytes, suggest a clear phagocytosis process.
F. Antunes; Mariana Hinzmann; M. Lopes-Lima; P. Vaz-Pires; S. Ferreira; B. Domingues; Jorge Machado; Paulo Martins Da Costa. Antibacterial effects of Anodonta cygnea fluids on Escherichia coli and enterococci multi-drug-resistant strains: environmental implications. Toxicological & Environmental Chemistry 2014, 96, 880 -889.
AMA StyleF. Antunes, Mariana Hinzmann, M. Lopes-Lima, P. Vaz-Pires, S. Ferreira, B. Domingues, Jorge Machado, Paulo Martins Da Costa. Antibacterial effects of Anodonta cygnea fluids on Escherichia coli and enterococci multi-drug-resistant strains: environmental implications. Toxicological & Environmental Chemistry. 2014; 96 (6):880-889.
Chicago/Turabian StyleF. Antunes; Mariana Hinzmann; M. Lopes-Lima; P. Vaz-Pires; S. Ferreira; B. Domingues; Jorge Machado; Paulo Martins Da Costa. 2014. "Antibacterial effects of Anodonta cygnea fluids on Escherichia coli and enterococci multi-drug-resistant strains: environmental implications." Toxicological & Environmental Chemistry 96, no. 6: 880-889.
This study reports on the intestinal permeability of salt‐containing mixtures of amino acids extracted from codfish salting wastewater. Permeability was evaluated in vitro using the Caco‐2 cell line model; cell integrity during exposure to mixtures of amino acids was estimated by measuring the transepithelial electrical resistance (TEER). The effect of salt (NaCl) on the permeability and on the intestinal cell's integrity was also examined. Permeation rate (i.e. transport) was ≥95% for all amino acids except for creatine, for which it was 6%. Values for apparent permeability coefficients, Papp > 10−5 cm s−1, for mixture with isotonic concentration of NaCl suggest that amino acids are very likely to be absorbed in humans. Mixture with a hypertonic level of NaCl exerts a cytotoxic effect in intestinal cells resulting in a loss of epithelium integrity. Results show that isotonic mixture of amino acids extracted from codfish salting wastewater could be used in food, feed, cosmetic and pharmaceutical formulations. These applications could contribute to the fish industry sustainability.
Vincenza Ferraro; Ruben Ferreira Jorge; Isabel B. Cruz; Filipa Antunes; Bruno Sarmento; Paula M. L. Castro; Manuela E. Pintado; Maria Manuela Pintado. In vitrointestinal absorption of amino acid mixtures extracted from codfish (Gadus morhuaL.) salting wastewater. International Journal of Food Science & Technology 2013, 49, 27 -33.
AMA StyleVincenza Ferraro, Ruben Ferreira Jorge, Isabel B. Cruz, Filipa Antunes, Bruno Sarmento, Paula M. L. Castro, Manuela E. Pintado, Maria Manuela Pintado. In vitrointestinal absorption of amino acid mixtures extracted from codfish (Gadus morhuaL.) salting wastewater. International Journal of Food Science & Technology. 2013; 49 (1):27-33.
Chicago/Turabian StyleVincenza Ferraro; Ruben Ferreira Jorge; Isabel B. Cruz; Filipa Antunes; Bruno Sarmento; Paula M. L. Castro; Manuela E. Pintado; Maria Manuela Pintado. 2013. "In vitrointestinal absorption of amino acid mixtures extracted from codfish (Gadus morhuaL.) salting wastewater." International Journal of Food Science & Technology 49, no. 1: 27-33.
Prediction of human intestinal absorption is a major goal in the design, optimization, and selection of drugs intended for oral delivery, in particular proteins, which possess intrinsic poor transport across intestinal epithelium. There are various techniques currently employed to evaluate the extension of protein absorption in the different phases of drug discovery and development. Screening protocols to evaluate protein absorption include a range of preclinical methodologies like in silico, in vitro, in situ, ex vivo and in vivo. It is the careful and critical use of these techniques that can help to identify drug candidates, which most probably will be well absorbed from the human intestinal tract. It is well recognized that the human intestinal permeability cannot be accurately predicted based on a single preclinical method. However, the present social and scientific concerns about the animal well care as well as the pharmaceutical industries need for rapid, cheap and reliable models predicting bioavailability give reasons for using methods providing an appropriate correlation between results of in vivo and in vitro drug absorption. The aim of this review is to describe and compare in silico, in vitro, in situ, ex vivo and in vivo methods used to predict human intestinal absorption, giving a special attention to the intestinal absorption of therapeutic peptides and proteins.
Filipa Antunes; Fernanda Andrade; Domingos Ferreira; Hanne Mørck Nielsen; Bruno Sarmento. Models to Predict Intestinal Absorption of Therapeutic Peptides and Proteins. Current Drug Metabolism 2012, 14, 4 -20.
AMA StyleFilipa Antunes, Fernanda Andrade, Domingos Ferreira, Hanne Mørck Nielsen, Bruno Sarmento. Models to Predict Intestinal Absorption of Therapeutic Peptides and Proteins. Current Drug Metabolism. 2012; 14 (1):4-20.
Chicago/Turabian StyleFilipa Antunes; Fernanda Andrade; Domingos Ferreira; Hanne Mørck Nielsen; Bruno Sarmento. 2012. "Models to Predict Intestinal Absorption of Therapeutic Peptides and Proteins." Current Drug Metabolism 14, no. 1: 4-20.
In vitro cell culture models for studying oral drug absorption during early stages of drug development have become a useful tool in drug discovery and development, with respect to substance throughput and reproducibility. The aim of this study was to establish an in vitro cellular model based on human colon carcinoma Caco-2, mucus-producing HT29, and Raji B cells in order to design a model that more accurately mimics the small intestinal epithelial layer. Normal oriented model was set up by seeding co-cultures of Caco-2 and HT29 cells into Transwell filters and maintained under identical conditions following addition of Raji B to the basolateral chamber. Inverted model was set up seeding Caco-2 and HT29 cells on the basolateral chamber and then transferred in the Transwell device with the epithelial cells facing the basolateral chamber following Raji B addition to the apical compartment. Morphological differences on size and thickness of cell membranes were detected between the models studied by using fluorescence microscopy. On the triple co-culture models, cell membranes were increasing in size and thickness from the Caco-2 to Caco-2/HT29 and Caco-2/Raji B. Also, the nuclei seem to be larger than in the other studied models. Insulin permeation was higher on the triple co-culture model when compared to the Caco-2/HT29 co-culture model. Also, insulin permeation as mediated by nanoparticles and insulin solution permeation was higher on the normal oriented Caco-2/HT29/Raji B model as compared to the inverted model. Overall, our results suggest that Caco-2/HT29/Raji B triple co-culture normal oriented cellular model may be reliable to obtain a more physiological, functional, and reproducible in vitro model of the intestinal barrier to study protein absorption, both in solution and when delivered by nanocarriers.
Filipa Antunes; Fernanda Andrade; Francisca Araújo; Domingos Ferreira; Bruno Sarmento. Establishment of a triple co-culture in vitro cell models to study intestinal absorption of peptide drugs. European Journal of Pharmaceutics and Biopharmaceutics 2012, 83, 427 -435.
AMA StyleFilipa Antunes, Fernanda Andrade, Francisca Araújo, Domingos Ferreira, Bruno Sarmento. Establishment of a triple co-culture in vitro cell models to study intestinal absorption of peptide drugs. European Journal of Pharmaceutics and Biopharmaceutics. 2012; 83 (3):427-435.
Chicago/Turabian StyleFilipa Antunes; Fernanda Andrade; Francisca Araújo; Domingos Ferreira; Bruno Sarmento. 2012. "Establishment of a triple co-culture in vitro cell models to study intestinal absorption of peptide drugs." European Journal of Pharmaceutics and Biopharmaceutics 83, no. 3: 427-435.
This chapter contains sections titled: Introduction Challenges on the Oral Delivery of Therapeutic Proteins Challenges on the Oral Delivery of Genetic Material Role of Chitosan in the Protection of Biopharmaceuticals in the Gastrointestinal Tract Chitosan‐Based Nanoparticles for Oral Delivery of Therapeutic Proteins Chitosan‐Based Nanoparticles for Oral Delivery of Genetic Material Conclusions and Future Perspectives Acknowledgments References
Filipa Antunes; Fernanda Andrade; Bruno Sarmento. Chitosan-Based Nanoparticulates for Oral Delivery of Biopharmaceuticals. Chitosan-Based Systems for Biopharmaceuticals 2012, 225 -241.
AMA StyleFilipa Antunes, Fernanda Andrade, Bruno Sarmento. Chitosan-Based Nanoparticulates for Oral Delivery of Biopharmaceuticals. Chitosan-Based Systems for Biopharmaceuticals. 2012; ():225-241.
Chicago/Turabian StyleFilipa Antunes; Fernanda Andrade; Bruno Sarmento. 2012. "Chitosan-Based Nanoparticulates for Oral Delivery of Biopharmaceuticals." Chitosan-Based Systems for Biopharmaceuticals , no. : 225-241.
Protein drugs represent a significant part of the new pharmaceuticals coming on the market every year and are now widely spread in therapy to treat or relief symptomatology related to many metabolic and oncologic diseases. The delivery of therapeutic proteins is still a major drawback against their maximum pharmacodynamic due to their physicochemical properties, poor stability, permeability and biodistribution. Despite the fact that the parenteral route remains the primary route of protein administration, research continues on non-parenteral delivery routes. However, the high molecular weight of proteins, combined with their hydrophilic and charged nature, renders transport through membranes very difficult. In this regard, the biopolymer chitosan exhibits several favorable biological properties, such as biocompatibility, biodegradability, low-toxicity and mucoadhesiveness, which made it a promising candidate for the formulation of protein drugs. The success of a protein formulation depends not only on the stability of the delivery system but also on their ability to maintain the native structure and activity of the protein during preparation and the delivery, as well as during longterm storage of the formulation. Chitosan-based delivery systems have been proposed as valid approaches to provide such protective conditions. The development of novel protein delivery systems based on chitosan is a rising subject irrespective of the intended route of administration. In this review, the different approaches recently exploited to formulate and deliver therapeutic proteins are underlined.
Fernanda Andrade; Filipa Antunes; Ana Vanessa Nascimento; Sara Baptista Da Silva; José Das Neves; Domingos Ferreira; Bruno Sarmento. Chitosan Formulations as Carriers for Therapeutic Proteins. Current Drug Discovery Technologies 2011, 8, 157 -172.
AMA StyleFernanda Andrade, Filipa Antunes, Ana Vanessa Nascimento, Sara Baptista Da Silva, José Das Neves, Domingos Ferreira, Bruno Sarmento. Chitosan Formulations as Carriers for Therapeutic Proteins. Current Drug Discovery Technologies. 2011; 8 (3):157-172.
Chicago/Turabian StyleFernanda Andrade; Filipa Antunes; Ana Vanessa Nascimento; Sara Baptista Da Silva; José Das Neves; Domingos Ferreira; Bruno Sarmento. 2011. "Chitosan Formulations as Carriers for Therapeutic Proteins." Current Drug Discovery Technologies 8, no. 3: 157-172.
The nosocomial prevalence of methicillin resistant Staphylococcus aureus (MRSA) in Portugal remains one of the highest in Europe and is currently around 50%. Transmission of S. aureus, including MRSA, occurs principally by direct human-to-human skin contact. However, S. aureus can survive for long periods on inanimate objects, which may represent an important reservoir for dissemination as well. Between May 2009 and February 2010, handrails of 85 public urban buses circulating in Oporto, Portugal, were screened for the occurrence of MRSA. Twenty-two (26%) buses showed MRSA contamination. The molecular characterization of a total of 55 MRSA, by pulsed-field gel electrophoresis (PFGE), staphylococcal cassette chromosome (SCC) mec typing, spa typing, and multilocus sequence typing (MLST), clustered the isolates into three clonal types. However, the overwhelming majority (n = 50; 91%) of the isolates belonged to a single clone (PFGE A, spa types t747, t032, t025 or t020, ST22, SCCmec type IVh) that exhibits the characteristics of the pandemic EMRSA-15, currently the major lineage circulating in Portuguese hospitals, namely in the Oporto region. Two additional clones were found but in much lower numbers: (i) PFGE B, ST5, spa type t002, SCCmec IVa (n = 3), and (ii) PFGE C, spa type t008, ST8, SCCmec IVa (n = 2). None of the 55 isolates was PVL positive. Public buses in Oporto seem to be an important reservoir of MRSA of nosocomial origin, providing evidence that the major hospital-associated MRSA clone in Portugal is escaping from the primary ecological niche of hospitals to the community environment. Infection control measures are urgently warranted to limit the spread of EMRSA-15 to the general population and future studies are required to assess the eventual increase of MRSA in the Portuguese community, which so far remains low.
Roméo Rocha Simões; Marta Aires-De-Sousa; Teresa Conceição; Filipa Antunes; Paulo Martins da Costa; Hermínia De Lencastre. High Prevalence of EMRSA-15 in Portuguese Public Buses: A Worrisome Finding. PLoS ONE 2011, 6, e17630 .
AMA StyleRoméo Rocha Simões, Marta Aires-De-Sousa, Teresa Conceição, Filipa Antunes, Paulo Martins da Costa, Hermínia De Lencastre. High Prevalence of EMRSA-15 in Portuguese Public Buses: A Worrisome Finding. PLoS ONE. 2011; 6 (3):e17630.
Chicago/Turabian StyleRoméo Rocha Simões; Marta Aires-De-Sousa; Teresa Conceição; Filipa Antunes; Paulo Martins da Costa; Hermínia De Lencastre. 2011. "High Prevalence of EMRSA-15 in Portuguese Public Buses: A Worrisome Finding." PLoS ONE 6, no. 3: e17630.
Bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. A complete understanding of the balance between Anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. Therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of A. cygnea collected from Mira Lagoon in northern Portugal. The results showed Vibrio metschnikovii and Aeromonas sobria as the dominant groups. The median for total bacteria from mucus was 3.1 × 10(3) CFU g(-1), whereas the range in means from fluids was 1.5 × 10(2) to 6.5 × 10(2) CFU ml(-1). During the experimental work, Escherichia coli and enterococci were not detected in healthy A. cygnea. However, the periodic detection of E. coli and enterococci in Mira lagoon revealed its presence in the water. Our observations suggest that A. cygnea has the ability to filter and eliminate E. coli, present in the surrounding environment, through an active phagocytic process conducted by hemolymph circulating cells, the hemocytes.
Filipa Antunes; Mariana Hinzmann; Manuel Lopes-Lima; Jorge Machado; Paulo Martins Da Costa. Association Between Environmental Microbiota and Indigenous Bacteria Found in Hemolymph, Extrapallial Fluid and Mucus of Anodonta cygnea (Linnaeus, 1758). Microbial Ecology 2010, 60, 304 -309.
AMA StyleFilipa Antunes, Mariana Hinzmann, Manuel Lopes-Lima, Jorge Machado, Paulo Martins Da Costa. Association Between Environmental Microbiota and Indigenous Bacteria Found in Hemolymph, Extrapallial Fluid and Mucus of Anodonta cygnea (Linnaeus, 1758). Microbial Ecology. 2010; 60 (2):304-309.
Chicago/Turabian StyleFilipa Antunes; Mariana Hinzmann; Manuel Lopes-Lima; Jorge Machado; Paulo Martins Da Costa. 2010. "Association Between Environmental Microbiota and Indigenous Bacteria Found in Hemolymph, Extrapallial Fluid and Mucus of Anodonta cygnea (Linnaeus, 1758)." Microbial Ecology 60, no. 2: 304-309.