Biological pairwise sequence alignment can be used as a method for arranging two biological sequence characters to identify regions of similarity. This operation has elicited considerable interest due to its significant influence on various critical aspects of life (e.g., identifying mutations in coronaviruses). Sequence alignment over large databases cannot yield results within a reasonable time, power, and cost. heuristic methods, such as FASTA, the BLAST family have been demonstrated to perform 40 times faster than DP-based (e.g., Needleman–Wunsch) techniques they cannot guarantee an optimum alignment result An optimized software platform of a widely used DNA sequence alignment algorithm called the Needleman–Wunsch (NW) algorithm based on a lookup table, is described in this study. This global alignment algorithm is the best approach for identifying similar regions between sequences. This study presents a new application of classical machine learning (ML) to global sequence alignment. Customized ML models are used to implement NW global alignment. An accuracy of 99.7% is achieved when using a multilayer perceptron with the ADAM optimizer, and up to 2912 Giga cell updates per second are realized on two real DNA sequences with a length of 4.1 M nucleotides. Our implementation is valid for RNA/DNA sequences. This study aims to parallelize the computation steps involved in the algorithm to accelerate its performance by using ML algorithms. All datasets used in this study are available from https://ieee-dataport.org/documents/dna-sequence-alignment-datasets-based-nw-algorithm.

Sound-based early fault detection for vehicles is a critical yet underexplored area, particularly within Intelligent Transportation Systems (ITSs) for smart cities. Despite the clear necessity for sound-based diagnostic systems, the scarcity of specialized publicly available datasets presents a major challenge. This study addresses this gap by contributing in multiple dimensions. Firstly, it emphasizes the significance of sound-based diagnostics for real-time detection of faults through analyzing sounds directly generated by vehicles, such as engine or brake noises, and the classification of external emergency sounds, like sirens, relevant to vehicle safety. Secondly, this paper introduces a novel dataset encompassing vehicle fault sounds, emergency sirens, and environmental noises specifically curated to address the absence of such specialized datasets. A comprehensive framework is proposed, combining audio preprocessing, feature extraction (via Mel Spectrograms, MFCCs, and Chromatograms), and classification using 11 models. Evaluations using both compact (52 features) and expanded (126 features) representations show that several classes (e.g., Engine Misfire, Fuel Pump Cartridge Fault, Radiator Fan Failure) achieve near-perfect accuracy, though acoustically similar classes like Universal Joint Failure, Knocking, and Pre-ignition Problem remain challenging. Logistic Regression yielded the highest accuracy of 86.5% for the vehicle fault dataset (DB1) using compact features, while neural networks performed best for datasets DB2 and DB3, achieving 88.4% and 85.5%, respectively. In the second scenario, a Bayesian-Optimized Weighted Soft Voting with Feature Selection (BOWSVFS) approach is proposed, significantly enhancing accuracy to 91.04% for DB1, 88.85% for DB2, and 86.85% for DB3. These results highlight the effectiveness of the proposed methods in addressing key ITS limitations and enhancing accessibility for individuals with disabilities through auditory-based vehicle diagnostics and emergency recognition systems.

Unrecognized mechanical faults and emergency sounds in vehicles can compromise safety, particularly for individuals with hearing impairments and in sound-insulated or autonomous driving environments. As intelligent transportation systems (ITSs) evolve, there is a growing need for inclusive, non-intrusive, and real-time diagnostic solutions that enhance situational awareness and accessibility. This study introduces an interpretable, sound-based machine learning framework to detect vehicle faults and emergency sound events using acoustic signals as a scalable diagnostic source. Three purpose-built datasets were developed: one for vehicular fault detection, another for emergency and environmental sounds, and a third integrating both to reflect real-world ITS acoustic scenarios. Audio data were preprocessed through normalization, resampling, and segmentation and transformed into numerical vectors using Mel-Frequency Cepstral Coefficients (MFCCs), Mel spectrograms, and Chroma features. To ensure performance and interpretability, feature selection was conducted using SHAP (explainability), Boruta (relevance), and ANOVA (statistical significance). A two-phase experimental workflow was implemented: Phase 1 evaluated 15 classical models, identifying ensemble classifiers and multi-layer perceptrons (MLPs) as top performers; Phase 2 applied advanced feature selection to refine model accuracy and transparency. Ensemble models such as Extra Trees, LightGBM, and XGBoost achieved over 91% accuracy and AUC scores exceeding 0.99. SHAP provided model transparency without performance loss, while ANOVA achieved high accuracy with fewer features. The proposed framework enhances accessibility by translating auditory alarms into visual/haptic alerts for hearing-impaired drivers and can be integrated into smart city ITS platforms via roadside monitoring systems.