Measurement of plant characteristics is still the primary bottleneck in both plant breeding and crop management. Rapid and accurate acquisition of information about large plant populations is critical for monitoring plant health and dissecting the underlying genetic traits. In recent years, high-throughput phenotyping technology has benefitted immensely from both remote sensing and machine learning. Simultaneous use of multiple sensors (e.g., high-resolution RGB, multispectral, hyperspectral, chlorophyll fluorescence, and light detection and ranging (LiDAR)) allows a range of spatial and spectral resolutions depending on the trait in question. Meanwhile, computer vision and machine learning methodology have emerged as powerful tools for extracting useful biological information from image data. Together, these tools allow the evaluation of various morphological, structural, biophysical, and biochemical traits. In this review, we focus on the recent development of phenomics approaches in strawberry farming, particularly those utilizing remote sensing and machine learning, with an eye toward future prospects for strawberries in precision agriculture. The research discussed is broadly categorized according to strawberry traits related to (1) fruit/flower detection, fruit maturity, fruit quality, internal fruit attributes, fruit shape, and yield prediction; (2) leaf and canopy attributes; (3) water stress; and (4) pest and disease detection. Finally, we present a synthesis of the potential research opportunities and directions that could further promote the use of remote sensing and machine learning in strawberry farming.

Biomass is a key biophysical parameter for precision agriculture and plant breeding. Fast, accurate and non-destructive monitoring of biomass enables various applications related to crop growth. In this paper, strawberry dry biomass weight was modeled using 4 canopy geometric parameters (area, average height, volume, standard deviation of height) and 25 spectral variables (5 band original reflectance values and 20 vegetation indices (VIs)) extracted from the Unmanned Aerial Vehicle (UAV) multispectral imagery. Six regression techniques—multiple linear regression (MLR), random forest (RF), support vector machine (SVM), multivariate adaptive regression splines (MARS), eXtreme Gradient Boosting (XGBoost) and artificial neural network (ANN)—were employed and evaluated for biomass prediction. The ANN had the highest accuracy in a five-fold cross-validation, with R² of 0.89~0.93, RMSE of 7.16~8.98 g and MAE of 5.06~6.29 g. As for the other five models, the addition of VIs increased the R² from 0.77~0.80 to 0.83~0.86, and reduced the RMSE from 8.89~9.58 to 7.35~8.09 g and the MAE from 6.30~6.70 to 5.25~5.47 g, respectively. Red-edge-related VIs, including the normalized difference red-edge index (NDRE), simple ratio vegetation index red-edge (SR_RedEdge), modified simple ratio red-edge (MSR_RedEdge) and chlorophyll index red and red-edge (CI_red&RE), were the most influential VIs for biomass modeling. In conclusion, the combination of canopy geometric parameters and VIs obtained from the UAV imagery was effective for strawberry dry biomass estimation using machine learning models.

Modeling plant canopy biophysical parameters at the individual plant level remains a major challenge. This study presents a workflow for automatic strawberry canopy delineation and biomass prediction from high-resolution images using deep neural networks. High-resolution (5 mm) RGB orthoimages, near-infrared (NIR) orthoimages, and Digital Surface Models (DSM), which were generated by Structure from Motion (SfM), were utilized in this study. Mask R-CNN was applied to the orthoimages of two band combinations (RGB and RGB-NIR) to identify and delineate strawberry plant canopies. The average detection precision rate and recall rate were 97.28% and 99.71% for RGB images and 99.13% and 99.54% for RGB-NIR images, and the mean intersection over union (mIoU) rates for instance segmentation were 98.32% and 98.45% for RGB and RGB-NIR images, respectively. Based on the center of the canopy mask, we imported the cropped RGB, NIR, DSM, and mask images of individual plants to vanilla deep regression models to model canopy leaf area and dry biomass. Two networks (VGG-16 and ResNet-50) were used as the backbone architecture for feature map extraction. The values of dry biomass models were about 0.76 and 0.79 for the VGG-16 and ResNet-50 networks, respectively. Similarly, the values of leaf area were 0.82 and 0.84, respectively. The RMSE values were approximately 8.31 and 8.73 g for dry biomass analyzed using the VGG-16 and ResNet-50 networks, respectively. Leaf area RMSE was 0.05 m² for both networks. This work demonstrates the feasibility of deep learning networks in individual strawberry plant extraction and biomass estimation.

Accurately monitoring aboveground biomass (AGB) and tree mortality is crucial for understanding forest health and carbon dynamics. LiDAR (Light Detection and Ranging) has emerged as a powerful tool for capturing forest structure across different spatial scales. However, the effectiveness of LiDAR for predicting AGB and tree mortality depends on the type of instrument, platform, and the resolution of the point cloud data. We evaluated the effectiveness of three distinct LiDAR-based approaches for predicting AGB and tree mortality in a 25.6 ha North American temperate forest. Specifically, we evaluated the following: GEDI-simulated waveforms from airborne laser scanning (ALS), grid-based structural metrics derived from unmanned aerial vehicle (UAV)-borne lidar data, and individual tree detection (ITD) from ALS data. Our results demonstrate varying levels of performance in the approaches, with ITD emerging as the most accurate for AGB modeling with a median R² value of 0.52, followed by UAV (0.38) and GEDI (0.11). Our findings underscore the strengths of the ITD approach for fine-scale analysis, while grid-based forest metrics used to analyze the GEDI and UAV LiDAR showed promise for broader-scale monitoring, if more uncertainty is acceptable. Moreover, the complementary strengths across scales of each LiDAR method may offer valuable insights for forest management and conservation efforts, particularly in monitoring forest dynamics and informing strategic interventions aimed at preserving forest health and mitigating climate change impacts.

These articles compare the performance of different deep learning models in tree detection and health classification. While the topic is interesting, there are several areas that need improvement. The main comments are as follows: * The abstract me...